##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9787466 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.314604822126586 31.0 31.0 34.0 28.0 34.0 2 31.329347044475046 31.0 31.0 34.0 28.0 34.0 3 31.389980409638206 31.0 31.0 34.0 28.0 34.0 4 34.98718401678228 37.0 35.0 37.0 32.0 37.0 5 35.03966634469024 37.0 35.0 37.0 32.0 37.0 6 35.1018587446434 37.0 35.0 37.0 32.0 37.0 7 35.010319524992475 37.0 35.0 37.0 32.0 37.0 8 35.01535136878125 37.0 35.0 37.0 32.0 37.0 9 36.558817675586305 39.0 37.0 39.0 32.0 39.0 10-11 36.50489437204686 39.0 37.0 39.0 32.0 39.0 12-13 36.396613689386 39.0 37.0 39.0 32.0 39.0 14-15 37.56812892121413 40.0 37.0 41.0 32.0 41.0 16-17 37.54075268307446 40.0 37.0 41.0 32.0 41.0 18-19 37.551332081255765 40.0 37.0 41.0 32.0 41.0 20-21 37.51751096759877 40.0 37.0 41.0 31.5 41.0 22-23 37.41073940895427 40.0 37.0 41.0 31.0 41.0 24-25 37.31418239409465 40.0 37.0 41.0 31.0 41.0 26-27 37.162835099503795 40.0 36.0 41.0 30.5 41.0 28-29 37.041471101917494 40.0 36.0 41.0 30.0 41.0 30-31 36.88841565324467 39.0 36.0 41.0 30.0 41.0 32-33 36.703441064316344 39.0 35.5 41.0 30.0 41.0 34-35 36.556307168780975 39.0 35.0 41.0 30.0 41.0 36-37 36.44905775407036 39.0 35.0 41.0 29.5 41.0 38-39 36.30962907048668 39.0 35.0 40.5 29.0 41.0 40-41 36.14004605482154 39.0 35.0 40.0 28.0 41.0 42-43 35.931560783965935 38.0 35.0 40.0 27.5 41.0 44-45 35.71058668300866 38.0 34.5 40.0 27.0 41.0 46-47 35.471634026621395 38.0 34.0 40.0 26.0 41.0 48-49 35.2693565423369 38.0 34.0 40.0 26.0 41.0 50-51 34.5894223285169 37.5 33.5 39.5 24.5 40.5 52-53 34.699936735412415 38.0 33.0 39.5 25.0 40.5 54-55 35.18691288429508 38.0 34.0 40.0 26.0 41.0 56-57 35.141139340867184 38.0 34.0 40.0 26.0 41.0 58-59 34.92323702580423 37.5 34.0 40.0 26.0 41.0 60-61 34.69390846415201 37.0 33.0 40.0 26.0 41.0 62-63 34.39201888415245 36.5 33.0 40.0 25.0 41.0 64-65 34.13967440602093 36.0 33.0 39.5 25.0 41.0 66-67 33.961378869668614 36.0 33.0 39.0 25.0 41.0 68-69 33.6647800360175 35.0 32.5 39.0 24.0 41.0 70-71 33.361908230383634 35.0 32.0 38.5 24.0 40.5 72-73 33.02876454436725 35.0 32.0 37.5 23.5 40.0 74-75 32.70242328300297 35.0 32.0 37.0 23.0 39.5 76-77 32.3764314481399 35.0 31.0 36.5 22.5 39.0 78-79 32.05127787927948 35.0 31.0 36.0 22.0 39.0 80-81 31.762101753405837 35.0 31.0 36.0 20.5 37.5 82-83 31.478494586852204 35.0 31.0 35.0 20.0 37.0 84-85 31.23531591323025 34.0 30.5 35.0 20.0 37.0 86-87 31.015926185592882 34.0 30.0 35.0 20.0 36.0 88-89 30.801297445120117 34.0 30.0 35.0 19.0 36.0 90-91 30.576882463755176 34.0 30.0 35.0 19.0 36.0 92-93 30.383169351495066 34.0 29.0 35.0 18.0 35.0 94-95 30.213347101282395 34.0 29.0 35.0 18.0 35.0 96-97 30.036032002563278 34.0 29.0 35.0 17.5 35.0 98-99 29.800619690530723 34.0 29.0 35.0 17.0 35.0 100 28.653458515207102 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4441901055380164 1101 2 -0.390784353603852 1101 3 -0.44732824076168853 1101 4 -0.40715094048788814 1101 5 -0.31170572387085116 1101 6 -0.3204774057322268 1101 7 -0.3421615365938777 1101 8 -0.3346168693691425 1101 9 -0.5327495607237651 1101 10-11 -0.4861789103163332 1101 12-13 -0.5584898726986864 1101 14-15 -0.6230499583192852 1101 16-17 -0.6740759277512467 1101 18-19 -0.6486962706707757 1101 20-21 -0.800988800959793 1101 22-23 -0.7791145459029707 1101 24-25 -0.8590130157825726 1101 26-27 -0.8851221110399266 1101 28-29 -0.8024981251965997 1101 30-31 -0.8754952442458332 1101 32-33 -0.9023748069458151 1101 34-35 -0.910400759922176 1101 36-37 -1.0627100295875138 1101 38-39 -1.0393225712822058 1101 40-41 -1.020027530879247 1101 42-43 -1.1617752931149 1101 44-45 -1.1457681066348826 1101 46-47 -1.1449907681576406 1101 48-49 -1.193725187256149 1101 50-51 -1.2228400546263956 1101 52-53 -1.0216271609869807 1101 54-55 -0.9976404944415549 1101 56-57 -0.889501812601921 1101 58-59 -0.882768058817355 1101 60-61 -0.9083375630902353 1101 62-63 -1.0281487456091227 1101 64-65 -0.9206077119746041 1101 66-67 -1.0156239234630462 1101 68-69 -1.0195249307546916 1101 70-71 -1.0604183899078592 1101 72-73 -1.0375144351054537 1101 74-75 -1.037283110968513 1101 76-77 -1.0673432291795741 1101 78-79 -1.141977660147905 1101 80-81 -1.109479375493482 1101 82-83 -1.0939293939371986 1101 84-85 -1.2262378638364986 1101 86-87 -1.3177452835976915 1101 88-89 -1.2990537342322206 1101 90-91 -1.4486219337287274 1101 92-93 -1.4788598204089958 1101 94-95 -1.5559630183017497 1101 96-97 -1.6234751723545635 1101 98-99 -1.6831905653213255 1101 100 -1.733455499557568 1102 1 -0.4363762170255576 1102 2 -0.37287160053454826 1102 3 -0.426300443719537 1102 4 -0.2494421901430499 1102 5 -0.4084762655345955 1102 6 -0.4070182963137867 1102 7 -0.3809021187683328 1102 8 -0.389730362926187 1102 9 -0.5273464747586019 1102 10-11 -0.5326521742625019 1102 12-13 -0.565607014324975 1102 14-15 -0.683043126024657 1102 16-17 -0.758945835833444 1102 18-19 -0.732124147618336 1102 20-21 -0.7170604727387584 1102 22-23 -0.721591959916239 1102 24-25 -0.7415237246276902 1102 26-27 -0.7786098128074102 1102 28-29 -0.8411637142847681 1102 30-31 -0.8379567861873554 1102 32-33 -0.9113748289525105 1102 34-35 -0.9899622721376318 1102 36-37 -0.9478542718769845 1102 38-39 -1.0835363406178686 1102 40-41 -1.134323255728276 1102 42-43 -1.0558281649099754 1102 44-45 -1.2073647514794956 1102 46-47 -1.1808800937056532 1102 48-49 -1.1308817440276613 1102 50-51 -1.1284755053783115 1102 52-53 -1.092544076319058 1102 54-55 -0.8976488951388717 1102 56-57 -0.8741805963035887 1102 58-59 -0.8481518511128741 1102 60-61 -0.7724371183710801 1102 62-63 -0.8760643132186274 1102 64-65 -0.9998464480055489 1102 66-67 -0.871598069482431 1102 68-69 -0.9956579637341463 1102 70-71 -1.0705793773829129 1102 72-73 -1.0600898340891831 1102 74-75 -1.0716017309540078 1102 76-77 -1.168332958049625 1102 78-79 -1.2008753305929787 1102 80-81 -1.1792617947076245 1102 82-83 -1.249954421065727 1102 84-85 -1.2265971656806371 1102 86-87 -1.2971725958657814 1102 88-89 -1.3582455994139053 1102 90-91 -1.3225920290177093 1102 92-93 -1.2981988728934617 1102 94-95 -1.43201074298344 1102 96-97 -1.4831377763676983 1102 98-99 -1.4997542467971314 1102 100 -1.6430004972148424 1103 1 -0.5668719079215414 1103 2 -0.504155299791158 1103 3 -0.6053910876709949 1103 4 -0.46296473381495673 1103 5 -0.4726118570759894 1103 6 -0.5463180473910043 1103 7 -0.5078798292875888 1103 8 -0.4888467745893621 1103 9 -0.6947935390360769 1103 10-11 -0.6239439267176934 1103 12-13 -0.6835009400317489 1103 14-15 -0.8524963129729173 1103 16-17 -0.8186652010669349 1103 18-19 -0.7266623896250408 1103 20-21 -0.7635367783845766 1103 22-23 -0.8239929030498274 1103 24-25 -0.8890347846639699 1103 26-27 -0.9790811627115161 1103 28-29 -0.9979685645643599 1103 30-31 -1.0784489091007217 1103 32-33 -1.0281395047258926 1103 34-35 -0.9916209226736115 1103 36-37 -0.9601453962284339 1103 38-39 -1.0358574576330284 1103 40-41 -1.1554276283682512 1103 42-43 -1.216352680436458 1103 44-45 -1.262332701941638 1103 46-47 -1.2430120469142878 1103 48-49 -1.338746937379355 1103 50-51 -1.3634427365654531 1103 52-53 -1.2343874543436613 1103 54-55 -1.1024350078312892 1103 56-57 -1.0072575932736356 1103 58-59 -0.9440490141881313 1103 60-61 -0.9553265517637755 1103 62-63 -0.9216874262061552 1103 64-65 -0.9524417097498841 1103 66-67 -1.0226893169308582 1103 68-69 -0.9370111227158517 1103 70-71 -1.0032620192106165 1103 72-73 -0.995585515521018 1103 74-75 -0.9967949611881046 1103 76-77 -1.0222878348998066 1103 78-79 -1.050792054005079 1103 80-81 -1.1343323246697672 1103 82-83 -1.0362896983344108 1103 84-85 -1.1175815345412694 1103 86-87 -1.2476412051112717 1103 88-89 -1.1918473595555632 1103 90-91 -1.2602885776175903 1103 92-93 -1.342860251311457 1103 94-95 -1.379405378264483 1103 96-97 -1.4829896822177524 1103 98-99 -1.490917633901006 1103 100 -1.4580410976075733 1104 1 -0.45042667313391505 1104 2 -0.4484348584737212 1104 3 -0.48385150635889573 1104 4 -0.2813057426497849 1104 5 -0.30065568288013367 1104 6 -0.3312369028122433 1104 7 -0.36587525765664 1104 8 -0.36430043559365544 1104 9 -0.5437656806859721 1104 10-11 -0.5193228456296524 1104 12-13 -0.448790552519732 1104 14-15 -0.6207130027928685 1104 16-17 -0.7603367935225407 1104 18-19 -0.7323291252943207 1104 20-21 -0.6623969518637907 1104 22-23 -0.6520436395072338 1104 24-25 -0.7357025092321408 1104 26-27 -0.6922490955345566 1104 28-29 -0.8182591829940478 1104 30-31 -0.7964408935743066 1104 32-33 -0.8528182580731354 1104 34-35 -0.9364213263804615 1104 36-37 -0.9534437512643379 1104 38-39 -0.9155568577259672 1104 40-41 -0.8560306426185349 1104 42-43 -0.8401812727499021 1104 44-45 -0.9260905031858471 1104 46-47 -1.0298547918299192 1104 48-49 -1.0117512446388446 1104 50-51 -0.9620136446481524 1104 52-53 -0.8783597175794853 1104 54-55 -0.7053303740979828 1104 56-57 -0.7210040724776619 1104 58-59 -0.7442449338059092 1104 60-61 -0.8596137963397936 1104 62-63 -0.9273672557447625 1104 64-65 -0.7886860648373357 1104 66-67 -0.8465350767618602 1104 68-69 -0.9430022953695314 1104 70-71 -0.8956174493837139 1104 72-73 -0.8515057214813453 1104 74-75 -0.7585537234807518 1104 76-77 -0.7697021074667774 1104 78-79 -0.9128877859856281 1104 80-81 -1.0367011355302829 1104 82-83 -0.9706739030127913 1104 84-85 -0.9284492953981633 1104 86-87 -0.9350082659657595 1104 88-89 -0.9644655899212005 1104 90-91 -1.02872786321608 1104 92-93 -1.081789774495089 1104 94-95 -1.0898678942577078 1104 96-97 -1.2325302017159778 1104 98-99 -1.1361585475870513 1104 100 -1.031169890153297 1105 1 -0.5582336132032388 1105 2 -0.5303068762004735 1105 3 -0.5535483176672749 1105 4 -0.4263549838549352 1105 5 -0.25389516111967936 1105 6 -0.3018088572409283 1105 7 -0.34653899428399626 1105 8 -0.4125364546637442 1105 9 -0.6166296075367796 1105 10-11 -0.594428776263527 1105 12-13 -0.5944499070522937 1105 14-15 -0.8160414572087902 1105 16-17 -0.7773527793714265 1105 18-19 -0.7614225718453156 1105 20-21 -0.7605371749874266 1105 22-23 -0.7600914970445984 1105 24-25 -0.7978515506656194 1105 26-27 -0.7533054427503956 1105 28-29 -0.7501784942139338 1105 30-31 -0.7635308883639453 1105 32-33 -0.8558998718363924 1105 34-35 -0.9134707350909039 1105 36-37 -0.848349537769586 1105 38-39 -0.8775100579001389 1105 40-41 -0.8818059307729129 1105 42-43 -0.9042752738792217 1105 44-45 -0.9879724415883473 1105 46-47 -1.0135100875487808 1105 48-49 -1.0861841960162053 1105 50-51 -0.9649865034407767 1105 52-53 -0.8432809149830263 1105 54-55 -0.7242179331878447 1105 56-57 -0.728563494670837 1105 58-59 -0.7438993769763442 1105 60-61 -0.7519672621353806 1105 62-63 -0.7879729128964144 1105 64-65 -0.7241525166554865 1105 66-67 -0.7400918180472615 1105 68-69 -0.7213571958664531 1105 70-71 -0.7296477182482235 1105 72-73 -0.5988579958080891 1105 74-75 -0.6592977330059213 1105 76-77 -0.647879975038002 1105 78-79 -0.7788285586665431 1105 80-81 -0.8061509125465811 1105 82-83 -0.9470887619667501 1105 84-85 -0.8684337085575784 1105 86-87 -0.9029008816987449 1105 88-89 -0.9903717287225895 1105 90-91 -0.9680484543492476 1105 92-93 -0.9448725910451046 1105 94-95 -1.0304706776853045 1105 96-97 -1.0644893160204383 1105 98-99 -0.9663457121134655 1105 100 -1.0328895459731378 1106 1 -0.6003086120206618 1106 2 -0.5559035238087588 1106 3 -0.6533698218537438 1106 4 -0.5011630379418293 1106 5 -0.5337410301013392 1106 6 -0.4605434479486519 1106 7 -0.5115067798126489 1106 8 -0.47886273127164714 1106 9 -0.6456780999169922 1106 10-11 -0.6336201625810176 1106 12-13 -0.7077714942052395 1106 14-15 -0.8881370435261644 1106 16-17 -0.8824882517425578 1106 18-19 -0.9376126221121339 1106 20-21 -0.8062937523330547 1106 22-23 -0.8849426016254966 1106 24-25 -0.8697866825937339 1106 26-27 -0.9258994930587008 1106 28-29 -0.9919442201587074 1106 30-31 -0.9666738511462754 1106 32-33 -0.9725582228281269 1106 34-35 -1.0285289721979254 1106 36-37 -0.9906497485089076 1106 38-39 -0.9898942578662826 1106 40-41 -1.119325227366076 1106 42-43 -1.0746043616683778 1106 44-45 -1.1123435355809121 1106 46-47 -1.0081511554045335 1106 48-49 -1.0810205999199596 1106 50-51 -1.1509712701203298 1106 52-53 -1.1318317494600123 1106 54-55 -0.9864195150754114 1106 56-57 -0.8788475599299943 1106 58-59 -0.9302035943785967 1106 60-61 -0.9346273699416727 1106 62-63 -0.825274497558631 1106 64-65 -0.8589772379955818 1106 66-67 -0.9446948220840099 1106 68-69 -0.967537840370305 1106 70-71 -0.9368451930097095 1106 72-73 -0.9112276425158328 1106 74-75 -0.8669631568216722 1106 76-77 -0.8067315886851247 1106 78-79 -0.9461303254926712 1106 80-81 -0.8359281846554332 1106 82-83 -0.9321593934168497 1106 84-85 -0.8677643678703113 1106 86-87 -0.9694355796300336 1106 88-89 -0.9755986327337745 1106 90-91 -0.9176164441992931 1106 92-93 -1.0388342433581315 1106 94-95 -1.1146275561027075 1106 96-97 -1.0621990950109428 1106 98-99 -1.110950929168709 1106 100 -1.323640816243774 1107 1 -0.5841631528226188 1107 2 -0.6088903701414132 1107 3 -0.6237107346197241 1107 4 -0.4903894051421034 1107 5 -0.47752347253678096 1107 6 -0.5366632305723797 1107 7 -0.46377885093749427 1107 8 -0.49312962459028853 1107 9 -0.6933163248458598 1107 10-11 -0.6226254862741669 1107 12-13 -0.7038941886662826 1107 14-15 -0.8801552985152981 1107 16-17 -0.8710807189244818 1107 18-19 -0.8794611359892102 1107 20-21 -0.8146843702917366 1107 22-23 -0.8912052236199202 1107 24-25 -0.9068965146892012 1107 26-27 -0.9052216947617993 1107 28-29 -0.9873694310279006 1107 30-31 -0.9653259254201956 1107 32-33 -1.0630087951275087 1107 34-35 -1.0843699384321823 1107 36-37 -1.10223007945509 1107 38-39 -1.0839977696105336 1107 40-41 -1.0697535873159154 1107 42-43 -1.056422699610053 1107 44-45 -1.0742041677529457 1107 46-47 -1.0086399651913354 1107 48-49 -0.9432370793847227 1107 50-51 -0.9880807363181106 1107 52-53 -0.8401432095876729 1107 54-55 -0.8008633376249534 1107 56-57 -0.8386431364395506 1107 58-59 -0.8383715990110971 1107 60-61 -0.8377746033893416 1107 62-63 -0.812221375353765 1107 64-65 -0.7038117601816083 1107 66-67 -0.8007354504176547 1107 68-69 -0.740917828411348 1107 70-71 -0.8278855097401774 1107 72-73 -0.7160268685596307 1107 74-75 -0.6556705158737586 1107 76-77 -0.7056703437879221 1107 78-79 -0.6483595924910368 1107 80-81 -0.7811365952502278 1107 82-83 -0.7775408559074961 1107 84-85 -0.7695847324129872 1107 86-87 -0.7832673852028655 1107 88-89 -0.7165519897505241 1107 90-91 -0.817561642170002 1107 92-93 -0.8443287989187489 1107 94-95 -0.8389913249620733 1107 96-97 -0.9695373440418429 1107 98-99 -0.9287053230686553 1107 100 -0.9532410765740664 1108 1 -0.623784985766136 1108 2 -0.6095150231246826 1108 3 -0.6141912024371514 1108 4 -0.5402324872635234 1108 5 -0.414559567765032 1108 6 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1.1224286787117421 2311 66-67 1.0872105563028 2311 68-69 1.1262024161788489 2311 70-71 1.041122281750198 2311 72-73 1.0665374669645615 2311 74-75 1.0779540883033718 2311 76-77 1.1075777980305617 2311 78-79 1.1192040225023128 2311 80-81 1.1433324823426965 2311 82-83 1.2148198658542633 2311 84-85 1.2372035125765102 2311 86-87 1.2327628500729766 2311 88-89 1.267238036301169 2311 90-91 1.250365521918141 2311 92-93 1.3241785613789503 2311 94-95 1.3849258897958983 2311 96-97 1.4033900602232485 2311 98-99 1.5183039485121839 2311 100 1.5635642922814377 2312 1 0.6543671855236255 2312 2 0.6241997899768563 2312 3 0.6705033172388326 2312 4 0.41417184368226856 2312 5 0.5262876936047007 2312 6 0.5523007294267046 2312 7 0.47685520976803986 2312 8 0.5906689115761239 2312 9 0.7688965651448783 2312 10-11 0.6904253287749853 2312 12-13 0.830725267787841 2312 14-15 1.0712137821670922 2312 16-17 1.06408439777357 2312 18-19 1.0897788537629793 2312 20-21 1.0763950449838617 2312 22-23 1.1406299919118084 2312 24-25 1.1060949147565466 2312 26-27 1.1450153981864943 2312 28-29 1.217083936759444 2312 30-31 1.1983016659813828 2312 32-33 1.2657613198494673 2312 34-35 1.1769574112694272 2312 36-37 1.224270544739852 2312 38-39 1.2771199794372592 2312 40-41 1.304813474593061 2312 42-43 1.3249352680426796 2312 44-45 1.3465678525823606 2312 46-47 1.3632580125518956 2312 48-49 1.424778697971142 2312 50-51 1.4257208543396018 2312 52-53 1.2783325112329464 2312 54-55 1.2092573288272206 2312 56-57 1.1464622388283914 2312 58-59 1.2052130515989177 2312 60-61 1.1971678875249054 2312 62-63 1.1433252793321529 2312 64-65 1.1660055677546453 2312 66-67 1.1493481501290432 2312 68-69 1.1621108186344102 2312 70-71 1.1777954414332825 2312 72-73 1.1417421298706145 2312 74-75 1.1584431441634209 2312 76-77 1.172631830987413 2312 78-79 1.1759025540279104 2312 80-81 1.156465007318321 2312 82-83 1.189705371299464 2312 84-85 1.1856731689061597 2312 86-87 1.169058054645756 2312 88-89 1.132650621343256 2312 90-91 1.2269665297534722 2312 92-93 1.2398363136592003 2312 94-95 1.345671795173633 2312 96-97 1.3740249175776746 2312 98-99 1.0409881678122588 2312 100 1.2685263595896856 2313 1 0.6517323807117101 2313 2 0.6557154281804998 2313 3 0.6845172398404564 2313 4 0.6181445697639134 2313 5 0.4363346624441675 2313 6 0.5745899602159312 2313 7 0.6599586251480147 2313 8 0.6577267563483673 2313 9 0.8001442947656372 2313 10-11 0.8379663360009033 2313 12-13 0.9182564308026144 2313 14-15 1.0986941000290784 2313 16-17 1.102238586573847 2313 18-19 1.0699457019986696 2313 20-21 1.0975189830288201 2313 22-23 1.10914310801342 2313 24-25 1.098745185583475 2313 26-27 1.1625984029451857 2313 28-29 1.1967666749982158 2313 30-31 1.216848422780842 2313 32-33 1.2316894300398928 2313 34-35 1.2728662928612238 2313 36-37 1.297626095832051 2313 38-39 1.3285194649620422 2313 40-41 1.3051890892010078 2313 42-43 1.3829693036983883 2313 44-45 1.389662110727727 2313 46-47 1.3645166954784145 2313 48-49 1.3421665980246118 2313 50-51 1.3444919115802278 2313 52-53 1.227356101779975 2313 54-55 1.1502481375043487 2313 56-57 1.1334739145477641 2313 58-59 1.0985600916620015 2313 60-61 1.0848031745385427 2313 62-63 1.0458248714685752 2313 64-65 1.06682930962657 2313 66-67 1.0734531080027594 2313 68-69 1.064074831180605 2313 70-71 1.0742014966117424 2313 72-73 1.0370509615316905 2313 74-75 1.0110946421063858 2313 76-77 0.8737011769299698 2313 78-79 1.0408216180158085 2313 80-81 1.1396083323251638 2313 82-83 1.1107189194820215 2313 84-85 1.0896395448232674 2313 86-87 1.214317354103816 2313 88-89 1.207739263079997 2313 90-91 1.2968954903723962 2313 92-93 1.359667520369488 2313 94-95 1.351408539638168 2313 96-97 1.390289814867483 2313 98-99 1.4636684770786061 2313 100 1.5887132582821373 2314 1 0.6801095708982245 2314 2 0.6685867991433803 2314 3 0.7072077190223247 2314 4 0.6085368702063789 2314 5 0.622141221448544 2314 6 0.6369020682169122 2314 7 0.6511767551799394 2314 8 0.648864973567342 2314 9 0.8227250872384388 2314 10-11 0.8264192660867806 2314 12-13 0.9147197288649664 2314 14-15 1.0575870772474616 2314 16-17 1.0718056639567095 2314 18-19 1.0676829337864149 2314 20-21 1.0547533273012348 2314 22-23 1.1158912167852577 2314 24-25 1.1102863611355858 2314 26-27 1.1995712565198886 2314 28-29 1.1918227493902478 2314 30-31 1.1911281752141036 2314 32-33 1.175961508603244 2314 34-35 1.1406045530677744 2314 36-37 1.1900838993469733 2314 38-39 1.190384251303648 2314 40-41 1.2885414510084985 2314 42-43 1.3511670986823958 2314 44-45 1.3796301644645013 2314 46-47 1.3905444334913568 2314 48-49 1.3385843805603201 2314 50-51 1.362566725858457 2314 52-53 1.3090837482741051 2314 54-55 1.1936236755011862 2314 56-57 1.1365623944622385 2314 58-59 1.130481248587337 2314 60-61 1.1211854609315068 2314 62-63 1.1439144969523127 2314 64-65 1.179537022891445 2314 66-67 1.1519926672323848 2314 68-69 1.1385029292329634 2314 70-71 1.1685320516670217 2314 72-73 1.1462564217208921 2314 74-75 1.1392449428264229 2314 76-77 1.173742302630508 2314 78-79 1.2034666351623535 2314 80-81 1.1833050283201167 2314 82-83 1.1797819066049975 2314 84-85 1.2451294364492291 2314 86-87 1.232859532345845 2314 88-89 1.3069436929347198 2314 90-91 1.3804603800333304 2314 92-93 1.4029811638812433 2314 94-95 1.3743892719413289 2314 96-97 1.464286994533147 2314 98-99 1.536869589998993 2314 100 1.599394954805664 2315 1 0.4354323573504004 2315 2 0.44632129380035224 2315 3 0.5101076885884162 2315 4 0.189838426057662 2315 5 0.3229762427102898 2315 6 0.38560929980457104 2315 7 0.40295226835613107 2315 8 0.47459324281944504 2315 9 0.5645861551195424 2315 10-11 0.4657529783250425 2315 12-13 0.5506500912261956 2315 14-15 0.7278494779040585 2315 16-17 0.7155656128842764 2315 18-19 0.61645213645496 2315 20-21 0.6987777618963733 2315 22-23 0.534016077558519 2315 24-25 0.7339374435136179 2315 26-27 0.8548313821748721 2315 28-29 0.7875077050248507 2315 30-31 0.867653883270556 2315 32-33 0.89466212245501 2315 34-35 0.9687088737495415 2315 36-37 0.9973317725245963 2315 38-39 1.0576978322100103 2315 40-41 1.0274654639717156 2315 42-43 1.0892364051440353 2315 44-45 1.117088866638504 2315 46-47 1.126372229231265 2315 48-49 1.1490405888079636 2315 50-51 1.0411574296638193 2315 52-53 1.0728714781813693 2315 54-55 0.9533838091650892 2315 56-57 0.9038275019083528 2315 58-59 0.9079224651075393 2315 60-61 0.9075958892097873 2315 62-63 0.8943353088741688 2315 64-65 0.9304900847793434 2315 66-67 0.8858130663243386 2315 68-69 0.8935605218899383 2315 70-71 0.8826785445212124 2315 72-73 0.6214635600268963 2315 74-75 0.8703196903566166 2315 76-77 0.9051930977948075 2315 78-79 0.9240811657344317 2315 80-81 0.6252091121218619 2315 82-83 0.6313735339079081 2315 84-85 0.9447446158219996 2315 86-87 1.0154465919281748 2315 88-89 1.0866061889704426 2315 90-91 1.15781662477929 2315 92-93 1.2170744289063187 2315 94-95 1.270561928525023 2315 96-97 1.0420980052124058 2315 98-99 0.5001380931153392 2315 100 0.9866114398014787 2316 1 0.31102855856204314 2316 2 0.3073168590840929 2316 3 0.30842740204823116 2316 4 0.16541879805325976 2316 5 0.11910941408104492 2316 6 0.21758795223929894 2316 7 0.17477171231526967 2316 8 0.18732965439590998 2316 9 0.35669916549302627 2316 10-11 0.2529243979899647 2316 12-13 0.11612963330756543 2316 14-15 0.1381756689214768 2316 16-17 0.27586446057494385 2316 18-19 0.31529635870300154 2316 20-21 0.33598723743787673 2316 22-23 0.3570679633046723 2316 24-25 0.3731182753516009 2316 26-27 0.48222923656181393 2316 28-29 0.4129303994617146 2316 30-31 0.3175545864221263 2316 32-33 0.4889072031597266 2316 34-35 0.4864135703763637 2316 36-37 0.5260921429067693 2316 38-39 0.6535595086722665 2316 40-41 0.7069586415280256 2316 42-43 0.631159544436457 2316 44-45 0.610558702569513 2316 46-47 0.5167909139295688 2316 48-49 0.44502960815428594 2316 50-51 0.4875681731929902 2316 52-53 0.6193437441957457 2316 54-55 0.29786745631274414 2316 56-57 0.22851567860143263 2316 58-59 0.22295844100879236 2316 60-61 0.26059575558765147 2316 62-63 0.46647285646658077 2316 64-65 0.3939112829405431 2316 66-67 0.34653587311296263 2316 68-69 0.35888372951058756 2316 70-71 0.08677095989410333 2316 72-73 0.013613831162928136 2316 74-75 0.2732268980137107 2316 76-77 0.44393097805433257 2316 78-79 0.48232385929193455 2316 80-81 0.6525349893486059 2316 82-83 0.634326619644451 2316 84-85 0.6794882149714034 2316 86-87 0.709344624700023 2316 88-89 0.6804676271563643 2316 90-91 0.5297224761437676 2316 92-93 0.616331805297353 2316 94-95 0.776066932736331 2316 96-97 0.7563826327399497 2316 98-99 0.6184923097962916 2316 100 0.4797303510814963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 37.0 1 610.0 2 231.0 3 227.0 4 161.0 5 125.0 6 152.0 7 578.0 8 2099.0 9 4133.0 10 6946.0 11 11072.0 12 16684.0 13 21442.0 14 25954.0 15 30883.0 16 36317.0 17 44504.0 18 53397.0 19 63758.0 20 77381.0 21 94159.0 22 104330.0 23 107035.0 24 107932.0 25 111376.0 26 122657.0 27 140471.0 28 163598.0 29 193853.0 30 234694.0 31 288755.0 32 364389.0 33 473237.0 34 661644.0 35 914536.0 36 1358424.0 37 1885321.0 38 1624214.0 39 439737.0 40 413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00233246261272 19.499595989996255 13.019610841861978 23.47846070552905 2 15.779604297299812 19.468341635539787 40.88523107552194 23.866822991638458 3 19.458106483310466 25.27408496812842 28.749496466014037 26.51831208254708 4 12.832667137275354 15.987509794696619 38.22284043545065 32.95698263257737 5 13.612426283120033 37.58122282161887 34.266733881207465 14.539617014053638 6 31.19210554077626 36.80281311768742 17.65834673828331 14.346734603253008 7 28.019598654493976 31.952200268741354 21.94997779571568 18.07822328104899 8 26.463337432070055 35.2891827594642 19.551556836775656 18.695922971690095 9 26.447506353499904 15.864513454374185 20.90719515465907 36.78078503746684 10-11 25.866854121933315 25.991413751540517 27.081015375880263 21.06071675064591 12-13 25.921595327000784 23.494182668739814 27.798654145479684 22.78556785877972 14-15 23.219341142848645 24.886822524853162 25.597916495177902 26.29591983712029 16-17 22.30495488535558 27.74547565967445 26.582722035419714 23.36684741955025 18-19 21.89007902213619 27.368316281478588 28.80112069206015 21.94048400432507 20-21 23.045450868839804 26.516998299871098 28.496118389019927 21.94143244226917 22-23 23.16413047094033 26.236568832010164 28.25141201669346 22.34788868035605 24-25 22.645632948170046 26.242985007635067 28.816123143787593 22.29525890040729 26-27 22.63147629916609 26.523917376711246 28.176341253461914 22.668265070660755 28-29 22.122626511092978 26.905986593564563 28.4684442005959 22.50294269474656 30-31 22.880354314982142 26.536391402746144 28.40636840734328 22.176885874928427 32-33 22.944715714688655 26.715399928872465 27.876097060470666 22.463787295968213 34-35 22.94786792627525 26.66862675726005 28.35812674203307 22.02537857443163 36-37 22.72628863737943 26.501892469665634 28.119268945097303 22.652549947857636 38-39 22.80452870587534 26.641901653011267 28.38074448227671 22.17282515883668 40-41 22.728625664792272 26.003526482162098 28.63691658715381 22.630931265891817 42-43 22.61135241393788 26.264555588873943 28.1744352686004 22.949656728587772 44-45 22.598630057736113 26.812627370348252 28.163036703307355 22.425705868608283 46-47 22.59001730410551 26.59563381697636 27.724762861383624 23.089586017534508 48-49 23.075427248576048 26.56204587678415 27.675795241219266 22.686731633420536 50-51 22.86239316143231 26.578302190789145 27.632581717304877 22.92672293047367 52-53 22.569861472678728 26.982294389070844 27.358930029135 23.08891410911543 54-55 22.435501900930436 26.86098979210014 27.50808906025444 23.195419246714984 56-57 22.973071523404688 26.666122224449847 27.19909469957394 23.161711552571525 58-59 22.488658662908946 26.88267422299243 27.704983769177442 22.92368334492118 60-61 22.630724539929027 27.167199360256717 27.334089579389232 22.86798652042503 62-63 22.803108120440893 26.79170325772776 27.129201184390432 23.27598743744091 64-65 22.358637349212465 26.77533059141585 27.35459468248421 23.51143737688747 66-67 22.61367611298585 26.576071056529464 27.2748981853368 23.535354645147887 68-69 22.276384455837007 26.643405122156327 27.457531923722918 23.622678498283747 70-71 22.39009762598044 26.3300629502952 27.509179346374836 23.770660077349522 72-73 22.678949776431605 26.486808524383182 27.490290434478272 23.34395126470694 74-75 22.78311569190047 26.692163630928505 27.26751372661238 23.257206950558647 76-77 22.585638750101488 26.55710128010969 27.101133463400583 23.756126506388235 78-79 22.423210071035513 26.574048942479138 27.210202658456446 23.7925383280289 80-81 22.25343136646231 26.835728489949396 27.393987742230898 23.516852401357397 82-83 22.77902163119513 26.304479987687845 27.992608618652326 22.923889762464704 84-85 22.907561258873404 26.531789246575876 27.215384897571777 23.345264596978936 86-87 22.135651376679284 26.933095933256574 27.521655399564192 23.40959729049995 88-89 22.615689818306787 26.17015225629392 27.500776390295567 23.713381535103725 90-91 22.64149130918355 26.12675976814679 27.52308780347651 23.70866111919315 92-93 22.730125723974623 26.274786001093037 27.486116283128904 23.50897199180344 94-95 22.0453496212008 26.256511429636447 27.79900456786897 23.899134381293784 96-97 22.21687736197729 26.198588742283356 27.5442876090759 24.040246286663454 98-99 21.932747331788995 26.415737328555917 27.40934102139071 24.24217431826438 100 22.439362651839293 26.154024613155052 26.900074147091274 24.50653858791438 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1398.0 1 1021.0 2 1270.0 3 1850.0 4 1407.5 5 1163.0 6 1750.5 7 3169.5 8 5102.0 9 6151.0 10 5636.5 11 5101.0 12 5494.0 13 6211.0 14 7311.5 15 8494.0 16 9515.5 17 10663.0 18 11699.0 19 12512.0 20 13412.0 21 14750.5 22 16569.0 23 19090.5 24 22564.0 25 26743.0 26 32586.5 27 39907.5 28 49916.0 29 65926.0 30 86685.5 31 107023.5 32 133795.5 33 161613.0 34 183202.5 35 206010.0 36 229637.0 37 263648.5 38 282970.5 39 279476.0 40 273814.0 41 266557.5 42 269311.0 43 285351.0 44 307590.5 45 343043.5 46 375877.0 47 400545.0 48 422899.0 49 428511.0 50 421552.5 51 425499.5 52 424103.0 53 406856.5 54 386311.0 55 346143.5 56 307752.0 57 266138.5 58 223033.0 59 199137.0 60 165649.5 61 126522.5 62 98818.0 63 79153.5 64 58795.0 65 37951.0 66 25790.5 67 18612.0 68 13989.5 69 10404.0 70 7798.5 71 5824.0 72 4382.0 73 3379.5 74 2509.5 75 1762.5 76 1237.5 77 822.0 78 531.5 79 403.0 80 307.5 81 235.5 82 169.0 83 127.0 84 102.5 85 76.5 86 56.0 87 51.0 88 44.0 89 34.5 90 28.5 91 22.5 92 23.5 93 19.5 94 14.5 95 14.0 96 12.0 97 7.5 98 6.5 99 4.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05650083484325769 2 0.13237338448991803 3 0.01667438742571366 4 0.015918318388028117 5 0.019259326162665596 6 0.051187917281143046 7 0.056909520809574204 8 0.048807321527349366 9 0.03966297303101743 10-11 0.03880984107633171 12-13 0.04027600198049219 14-15 0.018794445875980566 16-17 0.017675668043189114 18-19 0.017645016595715376 20-21 0.020756138514299822 22-23 0.024894083923254495 24-25 0.024030734819410867 26-27 0.019121394649033775 28-29 0.018314239865558662 30-31 0.016316787205186718 32-33 0.02274337402551386 34-35 0.022518596744039773 36-37 0.022850654091671942 38-39 0.020653967022720692 40-41 0.024117580587253124 42-43 0.031468819406371376 44-45 0.04074599084175618 46-47 0.04625814281245013 48-49 0.03930026423591152 50-51 0.02786216575362816 52-53 0.03956591011401726 54-55 0.03650587394122237 56-57 0.03833985221506772 58-59 0.04319810663965525 60-61 0.023407488720778188 62-63 0.025716564430466476 64-65 0.025180164099676057 66-67 0.032117608377898836 68-69 0.03875875533054214 70-71 0.04039349919580819 72-73 0.04424025585376235 74-75 0.026983490926047664 76-77 0.020592664127773216 78-79 0.019800835068034976 80-81 0.024587569448517112 82-83 0.020587555553194258 84-85 0.012991105154286104 86-87 0.014855734875605188 88-89 0.018339782738453447 90-91 0.020950264348300163 92-93 0.019433017698350114 94-95 0.02317760286472515 96-97 0.024158449183884777 98-99 0.027376851168627304 100 0.029231263740788476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9787466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.76975013896331 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97864980646824 47.69160864247804 2 14.300032472575399 17.952189305652393 3 4.636075838939985 8.730159661066427 4 1.9767943835793678 4.9633155813353165 5 1.0450694871966455 3.279937529459397 6 0.5662918482778102 2.1327598693277916 7 0.35413439352172454 1.5560249177880368 8 0.22495719998077285 1.129640578780313 9 0.16845619905864276 0.9516558171843424 >10 0.708441841641159 7.419152170966966 >50 0.02799976817948849 1.2001300815627245 >100 0.011901938409311854 1.3050189987681478 >500 7.337384900548197E-4 0.3026588269338282 >1k 3.7725029157924E-4 0.4085573049016529 >5k 2.095834746928016E-5 0.07602564802125862 >10k+ 6.287504240784049E-5 0.9011650657733582 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30070 0.3072296751784374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23728 0.24243251521895454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14703 0.15022274406879166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0217149157912784E-5 1.0217149157912784E-5 3 0.0 0.0 0.0 1.0217149157912784E-5 1.0217149157912784E-5 4 0.0 0.0 0.0 1.0217149157912784E-5 1.0217149157912784E-5 5 0.0 0.0 0.0 2.043429831582557E-5 1.0217149157912784E-5 6 0.0 0.0 0.0 2.043429831582557E-5 1.0217149157912784E-5 7 0.0 0.0 0.0 2.043429831582557E-5 1.0217149157912784E-5 8 0.0 0.0 0.0 2.043429831582557E-5 2.043429831582557E-5 9 0.0 0.0 0.0 2.043429831582557E-5 2.043429831582557E-5 10-11 0.0 0.0 0.0 3.065144747373835E-5 2.043429831582557E-5 12-13 0.0 0.0 0.0 3.065144747373835E-5 2.554287289478196E-5 14-15 0.0 0.0 0.0 4.086859663165114E-5 3.576002205269474E-5 16-17 0.0 0.0 0.0 5.619432036852031E-5 5.108574578956392E-5 18-19 0.0 0.0 0.0 8.173719326330227E-5 6.13028949474767E-5 20-21 0.0 0.0 0.0 1.4304008821077897E-4 6.641146952643309E-5 22-23 0.0 0.0 0.0 3.269487730532091E-4 9.195434242121505E-5 24-25 0.0 0.0 0.0 7.407433139486768E-4 1.1238864073704062E-4 26-27 0.0 0.0 0.0 0.001696046760213522 1.1238864073704062E-4 28-29 0.0 0.0 0.0 0.004975751639903526 1.1238864073704062E-4 30-31 0.0 0.0 0.0 0.016878730408871917 1.1238864073704062E-4 32-33 0.0 0.0 0.0 0.04369874694839297 1.1238864073704062E-4 34-35 0.0 0.0 0.0 0.08333106853193667 1.226057898949534E-4 36-37 0.0 0.0 0.0 0.14822529140841972 1.226057898949534E-4 38-39 0.0 0.0 0.0 0.24433801353690526 1.226057898949534E-4 40-41 0.0 0.0 0.0 0.3473626370707188 1.226057898949534E-4 42-43 0.0 0.0 0.0 0.44781764759131726 1.226057898949534E-4 44-45 0.0 0.0 0.0 0.5664234235909479 1.226057898949534E-4 46-47 0.0 0.0 0.0 0.7051774177299824 1.2771436447390978E-4 48-49 0.0 0.0 0.0 0.855757762019301 1.4304008821077897E-4 50-51 0.0 0.0 0.0 1.0121005784336825 1.4304008821077897E-4 52-53 0.0 0.0 0.0 1.1666298508725341 1.7369153568451732E-4 54-55 0.0 0.0 2.043429831582557E-5 1.332776021903933 2.0434298315825567E-4 56-57 0.0 0.0 2.043429831582557E-5 1.529788200541386 2.0945155773721206E-4 58-59 0.0 0.0 2.043429831582557E-5 1.753686807187887 2.1456013231616844E-4 60-61 0.0 0.0 2.043429831582557E-5 1.977529219514019 2.1456013231616844E-4 62-63 1.0217149157912784E-5 0.0 2.043429831582557E-5 2.2044061251400517 2.1456013231616844E-4 64-65 1.0217149157912784E-5 0.0 2.043429831582557E-5 2.435579341986986 2.1456013231616844E-4 66-67 1.0217149157912784E-5 0.0 2.043429831582557E-5 2.6942775586653376 2.1456013231616844E-4 68-69 1.0217149157912784E-5 0.0 2.043429831582557E-5 2.978099745122997 2.1456013231616844E-4 70-71 1.0217149157912784E-5 0.0 2.043429831582557E-5 3.2799858512918463 2.2477728147408125E-4 72-73 1.0217149157912784E-5 0.0 2.043429831582557E-5 3.584952427931806 2.452115797899068E-4 74-75 1.5325723736869175E-5 0.0 2.043429831582557E-5 3.88069291888217 2.452115797899068E-4 76-77 2.043429831582557E-5 0.0 2.043429831582557E-5 4.209061875668329 2.452115797899068E-4 78-79 2.043429831582557E-5 0.0 2.043429831582557E-5 4.567540771022857 2.5542872894781957E-4 80-81 2.043429831582557E-5 0.0 2.043429831582557E-5 4.948344137287425 2.6564587810573234E-4 82-83 2.043429831582557E-5 0.0 2.043429831582557E-5 5.335788650504636 2.8608017642155794E-4 84-85 2.043429831582557E-5 0.0 2.043429831582557E-5 5.711815499537878 2.8608017642155794E-4 86-87 2.043429831582557E-5 0.0 2.043429831582557E-5 6.108567835637947 2.8608017642155794E-4 88 2.043429831582557E-5 0.0 2.043429831582557E-5 6.433677521842732 3.065144747373835E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16165 0.0 48.50282 1 GTATCAA 28380 0.0 40.295963 1 CAACGCA 33190 0.0 32.788086 5 ATCAACG 33600 0.0 32.570038 3 GTACTAG 6305 0.0 32.394577 1 TATCAAC 35520 0.0 31.85096 2 AACGCAG 37145 0.0 29.765537 6 GTGGTAT 5740 0.0 29.59804 1 TCAACGC 37825 0.0 29.192842 4 GCATCGT 6420 0.0 28.042318 8 TACCTGG 6630 0.0 27.399004 2 ACGCAGA 40385 0.0 26.923689 7 GTACATG 37830 0.0 26.63471 1 CGCAGAG 41045 0.0 26.420284 8 TAGGCAT 8425 0.0 26.168287 5 TACATGG 37950 0.0 25.942446 2 CTGTGCG 8300 0.0 25.93793 9 TAGTACT 8510 0.0 25.133572 4 ACATGGG 38065 0.0 24.945953 3 CTAACGC 3200 0.0 24.385555 3 >>END_MODULE