FastQCFastQC Report
Mon 6 Jun 2016
ERR1211224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9787466
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274080.2800316241200736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231680.23671091169052338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145180.1483325714745778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169450.049.199081
GTATCAA289400.039.1731531
CAACGCA342700.031.9175975
ATCAACG345600.031.6881313
TATCAAC366900.030.7694532
GTGGTAT61500.030.2003381
GTACTAG61500.029.9709681
AACGCAG381800.029.1780956
TCAACGC386050.028.7209514
CTGTGCG88850.026.6521459
TAGTACT95900.026.2210224
TACCTGG61200.026.1877042
ACGCAGA418650.026.0262137
CGCAGAG426750.025.4218568
GGGCACC81750.025.12909994
GTACATG385950.024.6464651
CTAGTAC103000.024.2766553
TACATGG387350.024.0124632
CTAACGC30800.023.3482953
TAGGCAT84050.023.3109345
Produced by FastQC (version 0.11.5)