FastQCFastQC Report
Mon 6 Jun 2016
ERR1211223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6512649
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228920.351500595226305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173450.2663278797920785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115810.1778231868476253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122900.046.5226331
GTATCAA214200.040.0064771
TACCTGG44950.038.5257722
GTACTAG53900.034.748351
CAACGCA250200.033.12365
ATCAACG253450.032.6988533
TATCAAC271900.031.0835292
ACCTGGG55500.030.6611253
GCATCGT55400.030.375668
GTACATG297000.030.247491
AACGCAG282100.030.0611746
TACATGG304150.029.2110612
GTACCTG60100.029.1278421
TCAACGC287450.029.1087464
ACATGGG306650.028.237043
TAGGCAT70300.028.2182225
CTAGGCA74400.027.3577774
ACGCAGA304800.027.251737
CGCAGAG310450.026.7999578
GTGGTAT46950.026.6615831
Produced by FastQC (version 0.11.5)