FastQCFastQC Report
Mon 6 Jun 2016
ERR1211223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6512649
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220330.33831087780103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180550.27722974169189835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116220.1784527309855022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126400.047.1683921
GTATCAA224800.040.2845841
TACCTGG40950.040.0553252
GTACTAG49400.035.0267451
CAACGCA270450.032.3676535
ATCAACG272650.032.081623
ACCTGGG52350.030.7958983
TATCAAC292100.030.683712
TAGGCAT63500.030.0426125
AACGCAG302450.029.5489656
TCAACGC307150.028.7535654
GTACCTG58500.028.4528851
CTAGGCA69000.027.9967844
GTACATG309900.027.5077551
TACATGG313950.026.6020872
CTGTGCG72700.026.5635499
GTGGTAT47700.026.5162941
GGGCACC66100.026.38383594
ACGCAGA332650.026.3154437
CGCAGAG339000.025.7389558
Produced by FastQC (version 0.11.5)