FastQCFastQC Report
Mon 6 Jun 2016
ERR1211222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5413560
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164680.3041990852599768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129750.23967592489969633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84700.15645896600388653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.048.5054631
GTATCAA145950.040.276211
TACCTGG42600.035.9060862
CAACGCA170200.032.9759185
ATCAACG170550.032.9627573
TATCAAC184850.031.2660642
GTACCTG50550.031.1898961
AACGCAG187850.030.3783116
GTACTAG32900.030.3270571
TCAACGC189400.029.8067874
GTACATG179750.028.7490651
ACCTGGG52300.028.2209763
TACATGG180200.028.1812952
GCATCGT31250.027.376988
CTGTGCG40100.027.0784459
ACGCAGA206450.027.0499027
ACATGGG181400.026.793373
CGCAGAG209700.026.541018
TAGTACT42400.025.3876064
CATGGGG143150.024.824624
Produced by FastQC (version 0.11.5)