##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5413560 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.970225323077607 31.0 31.0 34.0 27.0 34.0 2 30.971159458840393 31.0 31.0 34.0 27.0 34.0 3 31.024779812175353 31.0 31.0 34.0 27.0 34.0 4 34.60703751320757 37.0 35.0 37.0 30.0 37.0 5 34.658560725289824 37.0 35.0 37.0 30.0 37.0 6 34.72567257036035 37.0 35.0 37.0 31.0 37.0 7 34.62501034439445 37.0 35.0 37.0 30.0 37.0 8 34.62521335313546 37.0 35.0 37.0 30.0 37.0 9 36.11994620914888 39.0 35.0 39.0 30.0 39.0 10-11 36.0545140351266 39.0 35.0 39.0 30.0 39.0 12-13 35.93881373809471 39.0 35.0 39.0 30.0 39.0 14-15 37.05073842351429 40.0 36.0 41.0 30.0 41.0 16-17 37.016068169559404 40.0 36.0 41.0 30.0 41.0 18-19 37.02577287404222 40.0 36.0 41.0 30.0 41.0 20-21 36.98507950406018 40.0 36.0 41.0 30.0 41.0 22-23 36.872582274880116 40.0 36.0 41.0 30.0 41.0 24-25 36.76886217202728 39.5 36.0 41.0 30.0 41.0 26-27 36.615895085673756 39.0 36.0 41.0 29.0 41.0 28-29 36.481934623427094 39.0 35.0 41.0 29.0 41.0 30-31 36.32065978764436 39.0 35.0 41.0 28.5 41.0 32-33 36.12377252676612 39.0 35.0 40.0 28.0 41.0 34-35 35.9601644758717 38.5 35.0 40.0 27.0 41.0 36-37 35.842743684377744 38.0 35.0 40.0 27.0 41.0 38-39 35.69547894546288 38.0 34.5 40.0 26.5 41.0 40-41 35.53010320380673 38.0 34.0 40.0 26.0 41.0 42-43 35.31915772615433 38.0 34.0 40.0 25.0 41.0 44-45 35.103221262902785 38.0 33.5 40.0 24.5 41.0 46-47 34.88011465652916 38.0 33.0 40.0 24.0 41.0 48-49 34.68545790570346 38.0 33.0 40.0 24.0 41.0 50-51 34.04109301457821 37.5 32.5 39.5 23.0 40.5 52-53 34.18329454185415 37.5 32.5 39.5 23.0 40.5 54-55 34.676125968863374 38.0 33.0 40.0 24.0 41.0 56-57 34.65165852045604 38.0 33.0 40.0 24.0 41.0 58-59 34.44644993313088 37.5 33.0 40.0 23.0 41.0 60-61 34.22859209097156 37.0 33.0 40.0 23.0 41.0 62-63 33.93407914570079 36.5 32.0 40.0 23.0 41.0 64-65 33.69069928106458 36.0 32.0 39.0 22.0 41.0 66-67 33.51343348923814 36.0 32.0 39.0 22.0 41.0 68-69 33.21267077486903 35.0 32.0 39.0 22.0 41.0 70-71 32.89384591655029 35.0 31.0 38.5 21.5 40.0 72-73 32.55022905444846 35.0 31.0 37.5 20.5 40.0 74-75 32.21903119204369 35.0 31.0 37.0 20.0 39.5 76-77 31.87967132533859 35.0 30.5 36.5 20.0 39.0 78-79 31.534021789728016 35.0 30.0 36.0 20.0 39.0 80-81 31.232678773302595 34.0 30.0 36.0 19.0 37.5 82-83 30.93989953006894 34.0 29.5 35.0 18.5 37.0 84-85 30.69009311802215 34.0 29.0 35.0 18.0 37.0 86-87 30.460986947590865 34.0 29.0 35.0 18.0 36.0 88-89 30.23374914104582 34.0 29.0 35.0 18.0 36.0 90-91 30.001232645431102 34.0 29.0 35.0 17.0 36.0 92-93 29.80070157160907 34.0 29.0 35.0 16.5 35.0 94-95 29.61718933566821 34.0 28.5 35.0 15.0 35.0 96-97 29.43171462032378 34.0 27.5 35.0 14.5 35.0 98-99 29.183753204915064 34.0 27.0 35.0 11.5 35.0 100 28.042779797397646 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5806344520688462 1101 2 -0.5113006566774771 1101 3 -0.5194342556609115 1101 4 -0.47642936191699903 1101 5 -0.34159931668208543 1101 6 -0.33692046650285334 1101 7 -0.462816953354519 1101 8 -0.5414838736564107 1101 9 -0.6660373298907771 1101 10-11 -0.6008698046215883 1101 12-13 -0.7045483563153496 1101 14-15 -0.8773476803980245 1101 16-17 -0.7070011646868437 1101 18-19 -0.808344068159812 1101 20-21 -0.9024986381313624 1101 22-23 -0.8831932971397976 1101 24-25 -1.017823218642178 1101 26-27 -0.9839873946629254 1101 28-29 -0.9246119011556502 1101 30-31 -1.0487847232729521 1101 32-33 -1.016755865052417 1101 34-35 -1.0530461731199665 1101 36-37 -1.044277634353051 1101 38-39 -1.0724037588273845 1101 40-41 -1.0475623202430953 1101 42-43 -1.178659712416291 1101 44-45 -1.1027630307531595 1101 46-47 -1.3669602552960285 1101 48-49 -1.1731659840405655 1101 50-51 -1.3290504650383497 1101 52-53 -1.1364819289640167 1101 54-55 -1.0410105239024645 1101 56-57 -0.9382520295108492 1101 58-59 -0.9575010870925382 1101 60-61 -0.8444979340206942 1101 62-63 -1.024732015019282 1101 64-65 -0.8975472581747468 1101 66-67 -0.8712422676394667 1101 68-69 -1.0278591383666225 1101 70-71 -1.0152396254054068 1101 72-73 -1.1700733455683654 1101 74-75 -1.1109007124951198 1101 76-77 -1.1107568159030734 1101 78-79 -1.1650930670190291 1101 80-81 -1.2079726655636307 1101 82-83 -1.1824261095255189 1101 84-85 -1.4704034201001654 1101 86-87 -1.351638044340966 1101 88-89 -1.4476267709406727 1101 90-91 -1.394860512523863 1101 92-93 -1.5548865615139924 1101 94-95 -1.5468829434457554 1101 96-97 -1.6723469042369885 1101 98-99 -1.7364692409627018 1101 100 -1.7960276507577895 1102 1 -0.41005553902583003 1102 2 -0.3529900010320617 1102 3 -0.41908271432166444 1102 4 -0.15434377940314192 1102 5 -0.2482523873578728 1102 6 -0.2818230108650823 1102 7 -0.2785872405910652 1102 8 -0.2903787957662516 1102 9 -0.46383605399200434 1102 10-11 -0.49239274679401035 1102 12-13 -0.5291515339533461 1102 14-15 -0.7459173821572165 1102 16-17 -0.7389005671005577 1102 18-19 -0.7293130161912629 1102 20-21 -0.7403668717359793 1102 22-23 -0.7398095099340551 1102 24-25 -0.7054737676865273 1102 26-27 -0.8275234144790886 1102 28-29 -0.8655523825811855 1102 30-31 -0.7588637712136901 1102 32-33 -0.7707388105813564 1102 34-35 -0.8627691760494827 1102 36-37 -0.844044994202811 1102 38-39 -0.9423828762351434 1102 40-41 -1.005653591829379 1102 42-43 -1.0428018585009653 1102 44-45 -1.2001767971188073 1102 46-47 -1.2013641879363917 1102 48-49 -1.130459638324254 1102 50-51 -1.0770507709481407 1102 52-53 -0.8838285013934666 1102 54-55 -0.7683926158580974 1102 56-57 -0.8710063860699577 1102 58-59 -0.8830554529988888 1102 60-61 -0.820207336710574 1102 62-63 -0.9163836942303689 1102 64-65 -0.8813534983096858 1102 66-67 -0.8036421299800622 1102 68-69 -0.7946408186186531 1102 70-71 -0.9294923663784118 1102 72-73 -0.9728872483187914 1102 74-75 -0.9039895091861965 1102 76-77 -0.9935602667355106 1102 78-79 -1.0242746520991304 1102 80-81 -1.0335906564585677 1102 82-83 -1.0386649910853158 1102 84-85 -0.9673988973088257 1102 86-87 -1.0010372502501248 1102 88-89 -1.1537826319811515 1102 90-91 -1.1153797094256745 1102 92-93 -1.218351499790316 1102 94-95 -1.141912907735037 1102 96-97 -1.1995394808259654 1102 98-99 -1.3800368289701908 1102 100 -1.4412856877941174 1103 1 -0.5020366738473214 1103 2 -0.47643849312347086 1103 3 -0.5096058812166184 1103 4 -0.42057948435654424 1103 5 -0.3888710694067612 1103 6 -0.41686854793302786 1103 7 -0.39741216717704475 1103 8 -0.40988101918605224 1103 9 -0.6256627594801571 1103 10-11 -0.5798400512873414 1103 12-13 -0.5779112923420016 1103 14-15 -0.8558857627740863 1103 16-17 -0.8767782511512223 1103 18-19 -0.7796138660557403 1103 20-21 -0.762388915485829 1103 22-23 -0.8182358094100266 1103 24-25 -0.8334561732712373 1103 26-27 -0.8285134537912455 1103 28-29 -0.9661665154973633 1103 30-31 -0.9289806265404579 1103 32-33 -1.0265668982739982 1103 34-35 -1.0591046708445901 1103 36-37 -0.9672687325253904 1103 38-39 -0.9415744279883853 1103 40-41 -1.0417384019675424 1103 42-43 -1.0514905356514248 1103 44-45 -1.074671090743287 1103 46-47 -1.0802590813477977 1103 48-49 -1.142938025785611 1103 50-51 -1.2538802583099553 1103 52-53 -1.1734368102554669 1103 54-55 -0.9841783974481686 1103 56-57 -0.8984646434111454 1103 58-59 -0.9036115033869834 1103 60-61 -0.850836098373442 1103 62-63 -0.8200975995235851 1103 64-65 -0.7958890168900226 1103 66-67 -1.0609907673609555 1103 68-69 -1.0211956947150895 1103 70-71 -0.9162906086001854 1103 72-73 -0.9322427850790262 1103 74-75 -1.0218316734090536 1103 76-77 -0.9425972976737711 1103 78-79 -1.0297825540341279 1103 80-81 -1.0833918502108695 1103 82-83 -1.0951705419420428 1103 84-85 -1.1340113403095238 1103 86-87 -1.222539262430491 1103 88-89 -1.2548466050955263 1103 90-91 -1.2793230922672123 1103 92-93 -1.3426438684746493 1103 94-95 -1.4725762245101919 1103 96-97 -1.6537172812246617 1103 98-99 -1.5302477357445703 1103 100 -1.6518113163769748 1104 1 -0.657167275976196 1104 2 -0.527052453637225 1104 3 -0.6592940938457055 1104 4 -0.5179184296716315 1104 5 -0.5635160180095085 1104 6 -0.49550237839695654 1104 7 -0.4901200820187128 1104 8 -0.5634249873011825 1104 9 -0.6158842303834149 1104 10-11 -0.6706175927778517 1104 12-13 -0.6827372257198974 1104 14-15 -0.837158923858631 1104 16-17 -0.9195602569164905 1104 18-19 -0.8850933214731356 1104 20-21 -0.7920296624663479 1104 22-23 -0.8631507009901753 1104 24-25 -0.8899939710975175 1104 26-27 -0.863612995826692 1104 28-29 -0.8883058735287577 1104 30-31 -1.1177634816307247 1104 32-33 -1.1145910066668492 1104 34-35 -1.1722407338550198 1104 36-37 -1.156420651238932 1104 38-39 -1.1984924583071574 1104 40-41 -1.103451436487937 1104 42-43 -1.063486433035294 1104 44-45 -1.1234653028961574 1104 46-47 -1.2453883988521568 1104 48-49 -1.203750186679521 1104 50-51 -1.3194001589438429 1104 52-53 -1.0334372030069119 1104 54-55 -0.9931960115322269 1104 56-57 -1.1390872526390083 1104 58-59 -1.0213529452920938 1104 60-61 -1.0424432863794664 1104 62-63 -1.138909698231842 1104 64-65 -1.0411760404641086 1104 66-67 -1.1560108835990235 1104 68-69 -1.2578286856727985 1104 70-71 -1.1939803218670875 1104 72-73 -1.0581721945526823 1104 74-75 -1.0932444412675864 1104 76-77 -1.2055261842970637 1104 78-79 -1.2389328444656442 1104 80-81 -1.240822134783837 1104 82-83 -1.2093828257344192 1104 84-85 -1.1114704348374325 1104 86-87 -1.0766120540013908 1104 88-89 -1.1467296600799095 1104 90-91 -1.226339735927919 1104 92-93 -1.2892583101089237 1104 94-95 -1.3680028794052248 1104 96-97 -1.4600497845978246 1104 98-99 -1.4724949449591769 1104 100 -1.2977387702757284 1105 1 -0.5248301840995424 1105 2 -0.5614939698571391 1105 3 -0.5544531048499621 1105 4 -0.3949578634295534 1105 5 -0.41628182453207074 1105 6 -0.3409772838570291 1105 7 -0.4530598950384572 1105 8 -0.4345210498991463 1105 9 -0.6424464542318233 1105 10-11 -0.5640728845021385 1105 12-13 -0.6334233854225957 1105 14-15 -0.7525323409023272 1105 16-17 -0.8002212421762351 1105 18-19 -0.7728119876094013 1105 20-21 -0.7846512514239663 1105 22-23 -0.871063056680569 1105 24-25 -0.8812192937024719 1105 26-27 -0.8883449373646854 1105 28-29 -0.8251159412933902 1105 30-31 -0.9172024274571129 1105 32-33 -0.8848145370642158 1105 34-35 -0.8765196144843728 1105 36-37 -0.832763123811354 1105 38-39 -0.9207705625075704 1105 40-41 -0.8603087009423405 1105 42-43 -1.0016351658995717 1105 44-45 -1.0336291907399513 1105 46-47 -1.0809240833175622 1105 48-49 -1.0675520338968028 1105 50-51 -1.0415041216784502 1105 52-53 -0.9947643805998183 1105 54-55 -0.8120436646235802 1105 56-57 -0.7573669433995462 1105 58-59 -0.8425324408592516 1105 60-61 -0.8460569953259522 1105 62-63 -0.8120271794714071 1105 64-65 -0.9367812066156702 1105 66-67 -0.9154805295954915 1105 68-69 -0.9473729062246292 1105 70-71 -0.9756745877531898 1105 72-73 -0.848969454993167 1105 74-75 -0.8596700425308299 1105 76-77 -0.8520867326178703 1105 78-79 -1.0200604617687468 1105 80-81 -0.8913489835963837 1105 82-83 -0.9074991110242188 1105 84-85 -0.9648178642722023 1105 86-87 -1.0611047584682645 1105 88-89 -1.0835983273861736 1105 90-91 -1.137596070967632 1105 92-93 -1.1230341914400377 1105 94-95 -1.1399233407199851 1105 96-97 -1.2110459768055968 1105 98-99 -1.1103030377894747 1105 100 -1.158730075661083 1106 1 -0.49738815893415733 1106 2 -0.49425282340865095 1106 3 -0.5046102438588989 1106 4 -0.4239616875893617 1106 5 -0.4601233667769975 1106 6 -0.4215628796329014 1106 7 -0.4351401207622345 1106 8 -0.42728673607609124 1106 9 -0.5866552314605826 1106 10-11 -0.5786072180134241 1106 12-13 -0.586191008647674 1106 14-15 -0.8912697456273477 1106 16-17 -0.7632754814460867 1106 18-19 -0.8276774637747053 1106 20-21 -0.8180562309557047 1106 22-23 -0.8261648464416496 1106 24-25 -0.7397214580928946 1106 26-27 -0.8797302511898195 1106 28-29 -0.8922444659276181 1106 30-31 -0.9337096825418385 1106 32-33 -0.8878887085515075 1106 34-35 -0.7751003560286094 1106 36-37 -0.9270601214612739 1106 38-39 -1.0576586119130411 1106 40-41 -1.0069997123839087 1106 42-43 -0.9008292667916891 1106 44-45 -0.9374880353986157 1106 46-47 -0.8818612019415966 1106 48-49 -0.9008301481518046 1106 50-51 -0.9028136917435958 1106 52-53 -0.8779089707299619 1106 54-55 -0.811052153311401 1106 56-57 -0.7831967819980861 1106 58-59 -0.8484773506297927 1106 60-61 -0.7898673988748968 1106 62-63 -0.7185941256867778 1106 64-65 -0.7877623246329364 1106 66-67 -0.7850159247958786 1106 68-69 -0.750104559901601 1106 70-71 -0.7158703802477078 1106 72-73 -0.7747049791967129 1106 74-75 -0.8541333721267073 1106 76-77 -0.8468039418312685 1106 78-79 -0.7164928725773478 1106 80-81 -0.708979922734823 1106 82-83 -0.7110169206676211 1106 84-85 -0.7904715876156772 1106 86-87 -0.9230733093267851 1106 88-89 -0.9137732222773849 1106 90-91 -1.0043549128293847 1106 92-93 -0.9262422224463016 1106 94-95 -1.0885253493894709 1106 96-97 -1.01629969617926 1106 98-99 -0.8300822527452105 1106 100 -0.9574134634962874 1107 1 -0.6686195204868852 1107 2 -0.5565218187065923 1107 3 -0.57127050143459 1107 4 -0.46812910239856365 1107 5 -0.3875695437331501 1107 6 -0.42782052133350845 1107 7 -0.5031687115944052 1107 8 -0.4643317802177478 1107 9 -0.7334814492271562 1107 10-11 -0.6590688128582158 1107 12-13 -0.7093594010349165 1107 14-15 -0.8156728693232296 1107 16-17 -0.8576259635826062 1107 18-19 -0.8849267647257335 1107 20-21 -0.8584468627506325 1107 22-23 -0.9626160415397038 1107 24-25 -0.8685703290061682 1107 26-27 -0.893686487601407 1107 28-29 -1.0239878540513914 1107 30-31 -1.020035193142654 1107 32-33 -1.124957188806924 1107 34-35 -1.1173705380816727 1107 36-37 -1.1264756522296508 1107 38-39 -1.0547360165976514 1107 40-41 -1.0168068098618548 1107 42-43 -1.0493499071503436 1107 44-45 -1.0434705768354817 1107 46-47 -0.910731722702856 1107 48-49 -0.9160503975980703 1107 50-51 -0.9859581159705257 1107 52-53 -0.8343741661652615 1107 54-55 -0.7846926584290372 1107 56-57 -0.9028565420642565 1107 58-59 -0.9561681908006818 1107 60-61 -0.9255931439058429 1107 62-63 -0.8323735447847085 1107 64-65 -0.8854462815474378 1107 66-67 -0.8831083845961771 1107 68-69 -0.768826503597964 1107 70-71 -0.8503774588625355 1107 72-73 -0.8497873841222372 1107 74-75 -0.8064364341197532 1107 76-77 -0.7976344653416874 1107 78-79 -0.6921180876431734 1107 80-81 -0.8432818874393639 1107 82-83 -0.8490827122097144 1107 84-85 -0.678313108888549 1107 86-87 -0.8131398431274768 1107 88-89 -0.8057235956740136 1107 90-91 -0.8641504704937049 1107 92-93 -0.9458563024065363 1107 94-95 -1.0814917361988847 1107 96-97 -1.0750123359607855 1107 98-99 -0.980653572224103 1107 100 -0.9901918239825847 1108 1 -0.6061236892549147 1108 2 -0.6075736837643646 1108 3 -0.5751182177344205 1108 4 -0.49107539749935825 1108 5 -0.4295591107215131 1108 6 -0.4241922972724552 1108 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1.1850516869562355 2311 74-75 1.2541050561773446 2311 76-77 1.2703787968437226 2311 78-79 1.2415404963639958 2311 80-81 1.1761944326728049 2311 82-83 1.305350395621108 2311 84-85 1.3140412604643004 2311 86-87 1.2733343421659455 2311 88-89 1.3874761574031282 2311 90-91 1.3916466671516687 2311 92-93 1.4110867879721845 2311 94-95 1.4279247828104467 2311 96-97 1.465400243010155 2311 98-99 1.6436024595783572 2311 100 1.6117638972794808 2312 1 0.6848611245396654 2312 2 0.6555195161873009 2312 3 0.7398272566883897 2312 4 0.4349353440232875 2312 5 0.5509504264665637 2312 6 0.6721291383506909 2312 7 0.5671892268362058 2312 8 0.6417653308389575 2312 9 0.8334043190136526 2312 10-11 0.7800477362042031 2312 12-13 0.9332311906183932 2312 14-15 1.2215724523324454 2312 16-17 1.172408606580447 2312 18-19 1.124481116158428 2312 20-21 1.1422774592218161 2312 22-23 1.188940149018265 2312 24-25 1.172156861938312 2312 26-27 1.1738425622727107 2312 28-29 1.2473793759934892 2312 30-31 1.2273758599138134 2312 32-33 1.2591884524961259 2312 34-35 1.340449229491142 2312 36-37 1.3332509459512707 2312 38-39 1.3321982918797204 2312 40-41 1.4185647684438791 2312 42-43 1.3963363028790639 2312 44-45 1.456620821467233 2312 46-47 1.5089698962376517 2312 48-49 1.4832541999863054 2312 50-51 1.3846048182039752 2312 52-53 1.2872806827698113 2312 54-55 1.158998356530951 2312 56-57 1.05149384400125 2312 58-59 1.1830891744698704 2312 60-61 1.1103748385868641 2312 62-63 1.136830038445531 2312 64-65 1.108084496022741 2312 66-67 1.0846369322022937 2312 68-69 1.1358664414674067 2312 70-71 1.2013974808536574 2312 72-73 1.2010556759670905 2312 74-75 1.1646134867598121 2312 76-77 1.2080370866370345 2312 78-79 1.2538071216696665 2312 80-81 1.1972454836785396 2312 82-83 1.249448228237906 2312 84-85 1.2700474629776828 2312 86-87 1.3130976232386402 2312 88-89 1.2986518103895683 2312 90-91 1.30760534253794 2312 92-93 1.390673902993413 2312 94-95 1.4586848584992396 2312 96-97 1.4080253188834924 2312 98-99 1.0495587841093368 2312 100 1.3724470075876418 2313 1 0.6291252840430097 2313 2 0.6138850395745408 2313 3 0.6040720157598116 2313 4 0.5894847289328595 2313 5 0.4007841030070338 2313 6 0.5570001767804627 2313 7 0.6121008014536784 2313 8 0.5918806602182158 2313 9 0.7269035343173158 2313 10-11 0.7981141684587598 2313 12-13 0.8761823087694367 2313 14-15 1.0905854169858813 2313 16-17 1.0612463631606062 2313 18-19 1.076079511966391 2313 20-21 1.0862310324161797 2313 22-23 1.1503143573592354 2313 24-25 1.0533743592918867 2313 26-27 1.116901197179466 2313 28-29 1.1011554394885508 2313 30-31 1.1139396212238708 2313 32-33 1.2067971432228646 2313 34-35 1.2143580926773012 2313 36-37 1.209137601733623 2313 38-39 1.1945973996006387 2313 40-41 1.250431720528141 2313 42-43 1.375492472567636 2313 44-45 1.3170147013661548 2313 46-47 1.3331085468542128 2313 48-49 1.333394540026191 2313 50-51 1.2486147708484552 2313 52-53 1.217200105328331 2313 54-55 1.0661408224403388 2313 56-57 1.0399008174319562 2313 58-59 1.0319337775136859 2313 60-61 1.0722766953662841 2313 62-63 1.0483736033966053 2313 64-65 1.1118391747063825 2313 66-67 1.0940299469567236 2313 68-69 1.0975629923254928 2313 70-71 1.1199308569851283 2313 72-73 1.156457278423808 2313 74-75 1.1161318010378594 2313 76-77 0.9127253915538773 2313 78-79 1.0959694569150287 2313 80-81 1.1542145968446995 2313 82-83 1.1818686924184654 2313 84-85 1.2038022812204723 2313 86-87 1.197614765586497 2313 88-89 1.2915965946085208 2313 90-91 1.27744717363408 2313 92-93 1.3649704937443836 2313 94-95 1.3953298219673727 2313 96-97 1.438398399092872 2313 98-99 1.4270657313317585 2313 100 1.6044906278613382 2314 1 0.6015278968085305 2314 2 0.6201267536648984 2314 3 0.6197680200680331 2314 4 0.5017545251093125 2314 5 0.5465095932031119 2314 6 0.5858981863468884 2314 7 0.6245864805579089 2314 8 0.6278579936604629 2314 9 0.8741939004251833 2314 10-11 0.7785047762988668 2314 12-13 0.8777330619514387 2314 14-15 1.0056233650149622 2314 16-17 1.038916136948842 2314 18-19 0.9823104071017923 2314 20-21 1.01714049627887 2314 22-23 1.0490123312255335 2314 24-25 1.0070630965056395 2314 26-27 1.038349291306794 2314 28-29 0.9666529571192726 2314 30-31 1.0643364796123436 2314 32-33 1.0819429056475727 2314 34-35 1.0771035779721743 2314 36-37 1.1394736609837324 2314 38-39 1.1279806768121574 2314 40-41 1.247486750162274 2314 42-43 1.3167961445378893 2314 44-45 1.2550327632120926 2314 46-47 1.2697598503628171 2314 48-49 1.2472066665740869 2314 50-51 1.334375319783348 2314 52-53 1.208667314152919 2314 54-55 1.0833483097864516 2314 56-57 1.0648632808620135 2314 58-59 1.0197907954635923 2314 60-61 0.926425564912563 2314 62-63 1.0362485803276655 2314 64-65 1.075369941865894 2314 66-67 1.1536082860535117 2314 68-69 1.0845460226615273 2314 70-71 1.0914254157405878 2314 72-73 1.0994164441298935 2314 74-75 1.0991811731088674 2314 76-77 1.1020774825831552 2314 78-79 1.0391613269665498 2314 80-81 1.0002727880582363 2314 82-83 1.0821168143797983 2314 84-85 1.1736303094538307 2314 86-87 1.2442436990299583 2314 88-89 1.273183144735441 2314 90-91 1.3107255892743694 2314 92-93 1.2739538326747741 2314 94-95 1.3495238431030607 2314 96-97 1.3839716978152303 2314 98-99 1.4689449620241888 2314 100 1.5027796234472284 2315 1 0.46498810585334027 2315 2 0.47528816377380423 2315 3 0.5081953383408866 2315 4 0.31960205754047877 2315 5 0.3214609114516165 2315 6 0.49390563744903204 2315 7 0.4885121643365409 2315 8 0.43028761300897855 2315 9 0.6050148487362037 2315 10-11 0.518646018514481 2315 12-13 0.6202015926111457 2315 14-15 0.8102542615213437 2315 16-17 0.776728118369789 2315 18-19 0.7042846813194004 2315 20-21 0.8118463421068043 2315 22-23 0.6085487679672568 2315 24-25 0.7815736796274351 2315 26-27 0.848020140087165 2315 28-29 0.8684012267469043 2315 30-31 0.8677887992548889 2315 32-33 0.8789949477000292 2315 34-35 0.9114565800236676 2315 36-37 0.9849166910986114 2315 38-39 0.9824898232835366 2315 40-41 1.0193367054191356 2315 42-43 1.142830234547688 2315 44-45 1.0893661635079397 2315 46-47 1.1580361011922378 2315 48-49 1.163975749370195 2315 50-51 1.1270147996672009 2315 52-53 1.135609269823732 2315 54-55 1.0776341058186176 2315 56-57 1.0024399803403128 2315 58-59 1.0047008648002347 2315 60-61 0.9490124487812892 2315 62-63 0.9880053481077411 2315 64-65 1.017028493892731 2315 66-67 0.974879185968895 2315 68-69 0.9328210803406023 2315 70-71 0.9381144965886961 2315 72-73 0.7187456257870366 2315 74-75 0.8912414150261796 2315 76-77 0.9959347306671162 2315 78-79 0.9971926776005589 2315 80-81 0.756933367223148 2315 82-83 0.7691133505913008 2315 84-85 1.018956946535237 2315 86-87 1.1422361748777945 2315 88-89 1.133133202871079 2315 90-91 1.2853058986412087 2315 92-93 1.2814670151598158 2315 94-95 1.310275503469164 2315 96-97 1.1493696804171485 2315 98-99 0.5314210290110601 2315 100 1.0368802255011538 2316 1 0.1364344748535764 2316 2 -0.013787522917709794 2316 3 0.06343841964105934 2316 4 -0.08142893963597686 2316 5 -0.07444932000293392 2316 6 -0.05219518285709768 2316 7 -0.12639886398345368 2316 8 -0.06186263103505496 2316 9 -0.023886796135762722 2316 10-11 -0.05893510156713688 2316 12-13 -0.24104047195967127 2316 14-15 -0.1819776515271201 2316 16-17 -0.094173710352635 2316 18-19 -0.0515395629542752 2316 20-21 -0.014815304620370284 2316 22-23 -0.060982610888046906 2316 24-25 -0.006040577754056642 2316 26-27 0.10221879163105996 2316 28-29 0.08893049544905551 2316 30-31 -0.020630042144325955 2316 32-33 0.17237234192595707 2316 34-35 0.11880081847623103 2316 36-37 0.10277608225292312 2316 38-39 0.23480047021456585 2316 40-41 0.3257654428072314 2316 42-43 0.2777987355410545 2316 44-45 0.19921804941709098 2316 46-47 0.03095994097035515 2316 48-49 -0.009738816857456811 2316 50-51 -0.00964467593451701 2316 52-53 0.26629695895276484 2316 54-55 -0.051451475486096854 2316 56-57 -0.21509238350533622 2316 58-59 -0.1627167742995539 2316 60-61 -0.06534472636584354 2316 62-63 0.09524464882944272 2316 64-65 0.04048414149415436 2316 66-67 -0.01695081829719669 2316 68-69 -0.0591674961690245 2316 70-71 -0.22302255164070317 2316 72-73 -0.30737435486100395 2316 74-75 -0.11336737484331394 2316 76-77 0.024170318071526253 2316 78-79 0.05028573545758874 2316 80-81 0.19646900922750277 2316 82-83 0.2686110536643689 2316 84-85 0.28452005683877957 2316 86-87 0.29696179478226625 2316 88-89 0.2644763935431129 2316 90-91 0.19728355172188827 2316 92-93 0.2951056406364465 2316 94-95 0.46442941577682006 2316 96-97 0.357654825885259 2316 98-99 0.22630817102040268 2316 100 0.07101411078544828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 24.0 1 328.0 2 130.0 3 112.0 4 90.0 5 65.0 6 93.0 7 381.0 8 1261.0 9 2559.0 10 4792.0 11 9153.0 12 14478.0 13 18896.0 14 22260.0 15 24810.0 16 28866.0 17 33485.0 18 39436.0 19 44901.0 20 51590.0 21 59434.0 22 64493.0 23 66667.0 24 67278.0 25 70345.0 26 77045.0 27 86641.0 28 100691.0 29 117959.0 30 141091.0 31 170401.0 32 211170.0 33 269414.0 34 369870.0 35 496664.0 36 716927.0 37 972391.0 38 844159.0 39 212969.0 40 241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24620318590273 19.301738482582923 12.752149838379765 23.699908493134583 2 15.769322184848924 19.594592719944732 41.14959728396729 23.48648781123906 3 19.436925002900622 25.457466565274295 28.939171228214867 26.166437203610222 4 12.684000911194222 15.946120561045857 38.20460744286053 33.165271084899395 5 13.249808407032884 37.46728878030107 34.55067846450708 14.73222434815897 6 30.96658498391449 36.620843868646446 18.12895056915179 14.28362057828727 7 27.723148152426543 31.98536172886854 22.281278445977478 18.01021167272744 8 26.140604618859626 35.54755490075118 19.981515247056613 18.33032523333258 9 26.53928366371937 15.792151268886759 20.919902938187683 36.748662129206195 10-11 25.631844037711065 26.018052053215936 27.661101565528874 20.68900234354413 12-13 25.591483054265396 23.692289752893743 28.11156274193821 22.60466445090265 14-15 22.9001527470685 25.084505110121537 26.00092285649489 26.01441928631507 16-17 22.147715174511383 27.829158285393607 26.933315473812325 23.089811066282685 18-19 21.73303195050296 27.34990988168085 29.056222727441945 21.860835440374245 20-21 22.839520257879542 26.67684936367536 28.732064844207784 21.751565534237315 22-23 22.80667783093642 26.54143000641236 28.476269336729853 22.17562282592137 24-25 22.34924172458083 26.358416469605935 29.106451782470817 22.185890023342424 26-27 22.32055576217485 26.679588532610715 28.48651582780773 22.513339877406708 28-29 21.962785389625296 27.10464153083415 28.69702490227162 22.235548177268928 30-31 22.6795061378052 26.739460496862556 28.670748190731253 21.910285174600993 32-33 22.670602865291713 26.850753792937738 28.240885600347433 22.237757741423124 34-35 22.686970885051565 26.879473573352747 28.52049206073528 21.913063480860405 36-37 22.4801931953888 26.74581228153529 28.323880713801568 22.450113809274335 38-39 22.510047441568716 26.920565973157245 28.53029983200761 22.039086753266428 40-41 22.53990367226954 26.300944015453826 28.834593360158273 22.324558952118366 42-43 22.35758011052378 26.541579828181288 28.37627938750158 22.724560673793352 44-45 22.322550553060385 27.078254496479985 28.383235292378384 22.215959658081246 46-47 22.34421095176343 26.822101345421263 27.922633426347236 22.911054276468075 48-49 22.693349368628724 26.82860393140698 27.92435630288377 22.55369039708053 50-51 22.556354092343724 26.918831056947628 27.860340944649174 22.664473906059467 52-53 22.291809477769423 27.21074820960036 27.700526191637092 22.796916120993124 54-55 22.209958472640228 27.054410579618317 27.89141610828417 22.844214839457294 56-57 22.71448600177363 26.902145742396712 27.536904230189485 22.846464025640177 58-59 22.239980544180817 27.09539754745428 27.958250561863473 22.70637134650143 60-61 22.433053386384987 27.425501193107966 27.62487909562139 22.516566324885652 62-63 22.532322243251954 27.085858310105625 27.493845592860623 22.887973853781798 64-65 22.140637298557706 26.995244095768467 27.667953261323447 23.196165344350376 66-67 22.330025597488774 26.82350925994192 27.559494805758984 23.286970336810327 68-69 22.057741405665592 26.891287620078234 27.79295541028393 23.258015563972243 70-71 22.15554504687052 26.597133790036104 27.84554524460135 23.401775918492028 72-73 22.403502812872723 26.829051037534413 27.7540964156894 23.01334973390346 74-75 22.47943770225902 26.987636430287736 27.502690473962243 23.030235393490997 76-77 22.335233211262988 26.799308927024402 27.444284504641423 23.421173357071183 78-79 22.19143329939018 26.784419823775806 27.57814092817517 23.446005948658847 80-81 22.02953830474346 27.049732731822495 27.69345636633713 23.227272597096906 82-83 22.52393284088588 26.624430403052052 28.195731372781417 22.65590538328065 84-85 22.588870186538852 26.794912936157495 27.55969248828763 23.056524389016026 86-87 21.965077853666035 27.141204441412114 27.807322037480535 23.086395667441316 88-89 22.348620510665505 26.442419300499793 27.783511863923284 23.425448324911414 90-91 22.414391980239564 26.402849292674585 27.8817503631917 23.301008363894155 92-93 22.460764867077962 26.572435342215496 27.786930466091796 23.179869324614742 94-95 21.904676913145128 26.55169518089218 28.025071048834427 23.51855685712826 96-97 22.02514930052965 26.462906841864676 27.87879986061219 23.633143996993482 98-99 21.812835841606606 26.625202453078607 27.71079718033247 23.851164524982323 100 22.235487111110455 26.321372636987643 27.418038754252628 24.025101497649278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1248.0 1 913.5 2 1211.5 3 1744.5 4 1278.5 5 978.0 6 1388.0 7 2478.5 8 3810.5 9 4380.0 10 3899.0 11 3378.0 12 3535.0 13 3951.0 14 4454.0 15 5117.5 16 5681.5 17 6097.0 18 6671.0 19 7365.0 20 7997.0 21 8774.0 22 10054.0 23 11838.5 24 14145.5 25 17133.5 26 21276.0 27 26165.5 28 31952.0 29 40536.0 30 52327.0 31 63993.5 32 78096.0 33 93599.0 34 106711.0 35 120014.5 36 133532.5 37 150584.0 38 159828.0 39 159914.0 40 159242.5 41 157025.5 42 158915.5 43 166632.0 44 176526.0 45 192046.5 46 207324.0 47 218623.0 48 228195.5 49 230395.0 50 226788.5 51 227232.5 52 223635.0 53 213484.0 54 202013.5 55 180930.5 56 159681.0 57 137085.5 58 115621.5 59 102210.0 60 85373.0 61 66444.0 62 52241.5 63 41745.0 64 30942.5 65 20434.0 66 14233.5 67 10433.5 68 7774.5 69 5859.0 70 4447.0 71 3266.5 72 2461.0 73 1923.0 74 1466.5 75 1061.5 76 717.5 77 453.0 78 286.0 79 217.5 80 164.0 81 124.0 82 101.0 83 69.0 84 43.5 85 38.0 86 44.0 87 37.0 88 23.0 89 18.5 90 17.0 91 14.0 92 9.5 93 9.5 94 11.5 95 8.0 96 4.5 97 3.0 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05609986773952815 2 0.13198338985806013 3 0.017068250836787622 4 0.016310893386237525 5 0.01928490678961718 6 0.05151877877034706 7 0.056986530120659976 8 0.048286155505803945 9 0.039955223549752845 10-11 0.03875453490863683 12-13 0.040333902275027894 14-15 0.018795395266700654 16-17 0.01750234596088341 18-19 0.01761317875852489 20-21 0.020513303630143566 22-23 0.025002031934623428 24-25 0.02422620235113308 26-27 0.019007824795513488 28-29 0.018130398480851788 30-31 0.016181588455655798 32-33 0.022748431715913373 34-35 0.022536002187100543 36-37 0.022452877588869432 38-39 0.02055024789602406 40-41 0.024152313819372096 42-43 0.03127332106783706 44-45 0.0409342465955859 46-47 0.046937689801165965 48-49 0.039973695682693086 50-51 0.02786521254036161 52-53 0.039317934963314344 54-55 0.035965242834659635 56-57 0.037923288926325745 58-59 0.04295694515254287 60-61 0.02262836285180177 62-63 0.025519251656950327 64-65 0.024752658139930103 66-67 0.03142109813135903 68-69 0.03856981357923436 70-71 0.039955223549752845 72-73 0.04370506653662285 74-75 0.026036471379277226 76-77 0.020448651164852703 78-79 0.019940667508995927 80-81 0.024004536755850125 82-83 0.019774418312533713 84-85 0.012801188127590716 86-87 0.014352847294571409 88-89 0.018389008342015234 90-91 0.020855038089538123 92-93 0.01965434944842211 94-95 0.023302595704120764 96-97 0.024420159747005666 98-99 0.02777285187566038 100 0.029777078299677108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5413560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.56797650973098 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40344820549278 56.63073172570824 2 11.897980329571416 16.55436832161731 3 3.3308355495390427 6.951584678043271 4 1.3042439935350205 3.629344620208081 5 0.6827547194087515 2.3748932140867995 6 0.3624841340106009 1.5130372631999793 7 0.22223586253412017 1.0822349485075433 8 0.15336341325790592 0.8535345864782532 9 0.10825667140367125 0.677807781590904 >10 0.5003131210865697 5.945660083701341 >50 0.022470211671961914 1.0734097335686985 >100 0.010832368753884696 1.3271251072149235 >500 4.6885218009874594E-4 0.21816481201919294 >1k 2.1879728795797356E-4 0.34369660602069485 >5k 3.1256755422544966E-5 0.18417754352740942 >10k+ 6.251351084508993E-5 0.6402289745073808 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16468 0.3041990852599768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12975 0.23967592489969633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8470 0.15645896600388653 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.8472132940246344E-5 0.0 0.0 0.0 3 0.0 1.8472132940246344E-5 0.0 0.0 0.0 4 0.0 1.8472132940246344E-5 0.0 0.0 3.694426588049269E-5 5 0.0 1.8472132940246344E-5 0.0 0.0 3.694426588049269E-5 6 0.0 1.8472132940246344E-5 0.0 0.0 5.5416398820739033E-5 7 0.0 1.8472132940246344E-5 0.0 0.0 5.5416398820739033E-5 8 0.0 1.8472132940246344E-5 0.0 0.0 7.388853176098538E-5 9 0.0 1.8472132940246344E-5 0.0 0.0 7.388853176098538E-5 10-11 0.0 1.8472132940246344E-5 0.0 0.0 8.312459823110854E-5 12-13 0.0 1.8472132940246344E-5 0.0 9.236066470123172E-6 1.015967311713549E-4 14-15 9.236066470123172E-6 1.8472132940246344E-5 0.0 1.8472132940246344E-5 1.1083279764147807E-4 16-17 1.8472132940246344E-5 1.8472132940246344E-5 0.0 1.8472132940246344E-5 1.293049305817244E-4 18-19 1.8472132940246344E-5 1.8472132940246344E-5 0.0 1.8472132940246344E-5 1.4777706352197076E-4 20-21 1.8472132940246344E-5 1.8472132940246344E-5 0.0 5.5416398820739033E-5 1.4777706352197076E-4 22-23 1.8472132940246344E-5 1.8472132940246344E-5 0.0 1.662491964622171E-4 1.7548526293234025E-4 24-25 1.8472132940246344E-5 1.8472132940246344E-5 0.0 5.35691855267144E-4 2.0319346234270978E-4 26-27 1.8472132940246344E-5 1.8472132940246344E-5 0.0 0.00133922963816786 2.0319346234270978E-4 28-29 1.8472132940246344E-5 1.8472132940246344E-5 0.0 0.004405603706248753 2.0319346234270978E-4 30-31 1.8472132940246344E-5 1.8472132940246344E-5 0.0 0.01656950324740097 2.0319346234270978E-4 32-33 3.694426588049269E-5 1.8472132940246344E-5 0.0 0.04524748963713342 2.2166559528295613E-4 34-35 3.694426588049269E-5 1.8472132940246344E-5 0.0 0.08592312637155587 2.955541270439415E-4 36-37 3.694426588049269E-5 1.8472132940246344E-5 0.0 0.15300467714406046 2.955541270439415E-4 38-39 3.694426588049269E-5 1.8472132940246344E-5 0.0 0.2511840637214698 3.047901935140647E-4 40-41 3.694426588049269E-5 1.8472132940246344E-5 0.0 0.3587750020319346 3.602065923348037E-4 42-43 3.694426588049269E-5 3.694426588049269E-5 0.0 0.4619788087690909 3.7867872527505006E-4 44-45 3.694426588049269E-5 3.694426588049269E-5 0.0 0.5809485809707475 3.879147917451732E-4 46-47 3.694426588049269E-5 3.694426588049269E-5 0.0 0.7247079555782148 4.1562299115554277E-4 48-49 3.694426588049269E-5 3.694426588049269E-5 0.0 0.8814901839085556 4.618033235061586E-4 50-51 3.694426588049269E-5 3.694426588049269E-5 0.0 1.0417451732316627 4.618033235061586E-4 52-53 6.465246529086221E-5 3.694426588049269E-5 0.0 1.1988044835561071 4.987475893866514E-4 54-55 7.388853176098538E-5 3.694426588049269E-5 0.0 1.3705399035015775 5.449279217372672E-4 56-57 7.388853176098538E-5 3.694426588049269E-5 0.0 1.5633335549989287 5.541639882073904E-4 58-59 7.388853176098538E-5 3.694426588049269E-5 0.0 1.786800183243559 5.541639882073904E-4 60-61 7.388853176098538E-5 3.694426588049269E-5 0.0 2.010119034424667 5.541639882073904E-4 62-63 7.388853176098538E-5 3.694426588049269E-5 0.0 2.2382591123031794 5.726361211476367E-4 64-65 7.388853176098538E-5 3.694426588049269E-5 0.0 2.470998751283813 5.726361211476367E-4 66-67 7.388853176098538E-5 5.5416398820739033E-5 0.0 2.729765625577254 5.818721876177599E-4 68-69 9.236066470123172E-5 5.5416398820739033E-5 0.0 3.0179586076445073 5.91108254087883E-4 70-71 9.236066470123172E-5 5.5416398820739033E-5 0.0 3.319959509084595 6.188164534982525E-4 72-73 9.236066470123172E-5 5.5416398820739033E-5 0.0 3.6200670168983073 7.758295834903464E-4 74-75 9.236066470123172E-5 5.5416398820739033E-5 0.0 3.9164708620575 7.758295834903464E-4 76-77 9.236066470123172E-5 5.5416398820739033E-5 0.0 4.247851690939049 7.758295834903464E-4 78-79 9.236066470123172E-5 5.5416398820739033E-5 0.0 4.606469679840992 7.758295834903464E-4 80-81 9.236066470123172E-5 5.5416398820739033E-5 0.0 4.987854572591788 7.943017164305928E-4 82-83 9.236066470123172E-5 5.5416398820739033E-5 0.0 5.363891782856383 8.497181152513319E-4 84-85 9.236066470123172E-5 5.5416398820739033E-5 0.0 5.74384508530431 8.681902481915782E-4 86-87 9.236066470123172E-5 5.5416398820739033E-5 0.0 6.1448381471711775 8.958984476019477E-4 88 9.236066470123172E-5 7.388853176098538E-5 0.0 6.464710837230953 9.051345140720709E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8490 0.0 48.505463 1 GTATCAA 14595 0.0 40.27621 1 TACCTGG 4260 0.0 35.906086 2 CAACGCA 17020 0.0 32.975918 5 ATCAACG 17055 0.0 32.962757 3 TATCAAC 18485 0.0 31.266064 2 GTACCTG 5055 0.0 31.189896 1 AACGCAG 18785 0.0 30.378311 6 GTACTAG 3290 0.0 30.327057 1 TCAACGC 18940 0.0 29.806787 4 GTACATG 17975 0.0 28.749065 1 ACCTGGG 5230 0.0 28.220976 3 TACATGG 18020 0.0 28.181295 2 GCATCGT 3125 0.0 27.37698 8 CTGTGCG 4010 0.0 27.078445 9 ACGCAGA 20645 0.0 27.049902 7 ACATGGG 18140 0.0 26.79337 3 CGCAGAG 20970 0.0 26.54101 8 TAGTACT 4240 0.0 25.387606 4 CATGGGG 14315 0.0 24.82462 4 >>END_MODULE