FastQCFastQC Report
Mon 6 Jun 2016
ERR1211222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5413560
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158650.2930603890970082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134820.2490412963004012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84330.15577549708509741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88400.047.7595061
GTATCAA153100.040.074771
TACCTGG42600.038.2820362
CAACGCA180700.032.816195
ATCAACG181500.032.7092173
GTACCTG51600.031.9810811
ACCTGGG52150.031.1863983
TATCAAC197300.030.752072
AACGCAG199900.030.4634516
TCAACGC200600.029.6182634
GTACATG193250.027.4669341
ACGCAGA219750.026.9633057
TACATGG195350.026.4879932
CGCAGAG224250.026.3591338
GTACTAG31300.026.1360911
ACATGGG194350.025.6368543
GTGGTAT38200.024.2459141
CTGTGCG41000.023.8375919
GCAGAGT246800.023.6841689
CATGGGG148250.023.399424
Produced by FastQC (version 0.11.5)