##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211222_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5413560 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.170147001233936 33.0 31.0 34.0 30.0 34.0 2 32.30902770081056 34.0 31.0 34.0 31.0 34.0 3 32.38651257213368 34.0 31.0 34.0 30.0 34.0 4 35.99634270239916 37.0 35.0 37.0 35.0 37.0 5 35.885534287973165 37.0 35.0 37.0 35.0 37.0 6 35.91203237795462 37.0 35.0 37.0 35.0 37.0 7 35.84785944923488 37.0 35.0 37.0 35.0 37.0 8 35.833273114179946 37.0 35.0 37.0 35.0 37.0 9 37.44167867355308 39.0 37.0 39.0 34.0 39.0 10-11 37.41962054544514 39.0 37.0 39.0 34.0 39.0 12-13 37.32705003731371 39.0 37.0 39.0 34.0 39.0 14-15 38.56587772186879 40.0 38.0 41.0 34.0 41.0 16-17 38.51797126105557 40.0 38.0 41.0 34.0 41.0 18-19 38.53582374629634 40.0 38.0 41.0 34.0 41.0 20-21 38.49384240684503 40.0 38.0 41.0 34.0 41.0 22-23 38.36842733801787 40.0 38.0 41.0 34.0 41.0 24-25 38.31340291785812 40.0 38.0 41.0 34.0 41.0 26-27 38.181093033050345 40.0 38.0 41.0 33.5 41.0 28-29 38.15442777026578 40.0 38.0 41.0 33.5 41.0 30-31 38.16205158897287 40.0 37.5 41.0 34.0 41.0 32-33 38.03075406941089 40.0 37.0 41.0 33.0 41.0 34-35 37.89261973636572 40.0 37.0 41.0 33.0 41.0 36-37 37.74975792269782 40.0 37.0 41.0 33.0 41.0 38-39 37.66345223845307 40.0 37.0 41.0 33.0 41.0 40-41 37.56572735870665 40.0 37.0 41.0 32.5 41.0 42-43 37.42558870687681 40.0 36.0 41.0 32.0 41.0 44-45 37.292442219168166 40.0 36.0 41.0 31.5 41.0 46-47 37.06625141311817 39.5 36.0 41.0 31.0 41.0 48-49 36.84430282475857 39.0 35.5 41.0 31.0 41.0 50-51 36.65061632271555 39.0 35.0 41.0 30.0 41.0 52-53 36.45550857476411 39.0 35.0 40.5 30.0 41.0 54-55 36.226538266870605 38.5 35.0 40.0 29.5 41.0 56-57 35.97719485883596 38.0 35.0 40.0 29.0 41.0 58-59 35.71084314203593 38.0 34.5 40.0 28.5 41.0 60-61 35.45402563193167 37.5 34.0 40.0 28.0 41.0 62-63 35.14960312622378 37.0 34.0 40.0 28.0 41.0 64-65 34.77505837194009 36.5 34.0 39.5 27.0 41.0 66-67 34.46942501791797 36.0 33.5 39.0 26.5 40.5 68-69 34.13917200511308 35.5 33.0 39.0 26.0 40.0 70-71 33.8121022949778 35.0 33.0 38.0 26.0 40.0 72-73 33.46840489437635 35.0 33.0 37.5 26.0 40.0 74-75 33.1250361130199 35.0 32.0 37.0 26.0 39.0 76-77 32.12826448769387 34.0 31.0 36.0 24.5 38.5 78-79 32.61646421208964 35.0 32.0 36.0 25.5 38.5 80-81 32.54049470219228 35.0 32.0 36.0 25.5 37.5 82-83 32.28336676789395 35.0 32.0 35.0 25.0 37.0 84-85 32.02963105608878 34.0 32.0 35.0 25.0 36.5 86-87 31.788856685803797 34.0 31.0 35.0 24.5 36.0 88-89 31.57171085200866 34.0 31.0 35.0 24.0 36.0 90-91 31.362039582086467 34.0 31.0 35.0 24.0 35.5 92-93 31.183185186827153 34.0 31.0 35.0 23.0 35.0 94-95 30.994463994118473 34.0 31.0 35.0 23.0 35.0 96-97 30.820184222581815 34.0 30.0 35.0 21.0 35.0 98-99 30.649815278670598 34.0 30.0 35.0 20.0 35.0 100 28.887292096143756 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.40686675826754737 1101 2 -0.3412130545758352 1101 3 -0.4460609150867505 1101 4 -0.2635987615195674 1101 5 -0.26175743387203454 1101 6 -0.3955104300430179 1101 7 -0.38908275015216276 1101 8 -0.3895950676039419 1101 9 -0.4866457977332672 1101 10-11 -0.49909357625965356 1101 12-13 -0.5648983978332751 1101 14-15 -0.8170979900527016 1101 16-17 -0.7641576500719651 1101 18-19 -0.7353826251562623 1101 20-21 -0.7916329940894826 1101 22-23 -0.795444966061666 1101 24-25 -0.7823269098403216 1101 26-27 -0.8806102099323638 1101 28-29 -1.001545500948282 1101 30-31 -0.8329788285807851 1101 32-33 -0.781108597345181 1101 34-35 -0.8126380567151514 1101 36-37 -0.7480469118491513 1101 38-39 -0.826811934848287 1101 40-41 -0.9116274628631515 1101 42-43 -0.8137826339958494 1101 44-45 -0.9421739582342923 1101 46-47 -0.9334376631144039 1101 48-49 -0.9729074241053084 1101 50-51 -1.0137287053946196 1101 52-53 -0.9570818559031338 1101 54-55 -0.8769912187453528 1101 56-57 -0.8606639770017708 1101 58-59 -0.9555031950772772 1101 60-61 -1.0581904019041062 1101 62-63 -1.0505476163058631 1101 64-65 -1.0371879961498038 1101 66-67 -1.1435382427818368 1101 68-69 -1.1018379172321815 1101 70-71 -1.1382430150024376 1101 72-73 -1.188055694662495 1101 74-75 -1.2630559857727448 1101 76-77 -1.1957637384093545 1101 78-79 -1.0085744429492287 1101 80-81 -0.9519766117204682 1101 82-83 -0.8661009144353571 1101 84-85 -0.8729207563734391 1101 86-87 -1.01203556683258 1101 88-89 -0.9119652910082472 1101 90-91 -1.0661542527256174 1101 92-93 -1.1502346618749293 1101 94-95 -1.0957810915757875 1101 96-97 -1.1268013107961181 1101 98-99 -1.199067490222653 1101 100 -1.131735202471262 1102 1 -0.4200444599927522 1102 2 -0.36219115865739226 1102 3 -0.34579056730555635 1102 4 -0.28174388597599176 1102 5 -0.2946654676155802 1102 6 -0.35584374425698684 1102 7 -0.3548973305777565 1102 8 -0.32569569493146133 1102 9 -0.5006945351118333 1102 10-11 -0.48080845558460794 1102 12-13 -0.5074022695041123 1102 14-15 -0.6278746610318464 1102 16-17 -0.6996082190854267 1102 18-19 -0.7045400561088115 1102 20-21 -0.7332350054463888 1102 22-23 -0.6823694467054437 1102 24-25 -0.7501868796317268 1102 26-27 -0.8968359203759988 1102 28-29 -0.9446503366598478 1102 30-31 -0.7994283205368404 1102 32-33 -0.6979760810459226 1102 34-35 -0.7210978858730286 1102 36-37 -0.8329372410633269 1102 38-39 -0.8273232928116059 1102 40-41 -0.8451726812168872 1102 42-43 -0.805082304505504 1102 44-45 -0.9021703467992168 1102 46-47 -0.9386965711438933 1102 48-49 -0.8659040867144299 1102 50-51 -0.9472796808007402 1102 52-53 -0.9395805016149836 1102 54-55 -0.927024040317086 1102 56-57 -0.9748578728267461 1102 58-59 -1.0285698982831732 1102 60-61 -1.0711559636840846 1102 62-63 -0.9846540516707378 1102 64-65 -0.9875610573933642 1102 66-67 -1.0732507937933562 1102 68-69 -1.0552869439504278 1102 70-71 -1.0438335589907766 1102 72-73 -1.0657756682352826 1102 74-75 -1.1271294721034621 1102 76-77 -0.9878721154516725 1102 78-79 -0.7590980797918121 1102 80-81 -0.8122383982761576 1102 82-83 -0.7928754143047101 1102 84-85 -0.8229945358677071 1102 86-87 -0.8779746355496911 1102 88-89 -0.8813397054777816 1102 90-91 -0.9701481812659409 1102 92-93 -1.001103849323279 1102 94-95 -1.022459930903043 1102 96-97 -1.1666331666276264 1102 98-99 -1.3007322980624387 1102 100 -1.3748377226156343 1103 1 -0.30095182484733485 1103 2 -0.3271626704414743 1103 3 -0.32797959000713917 1103 4 -0.2297886321435314 1103 5 -0.2330466749570661 1103 6 -0.3047458397688132 1103 7 -0.37936010461726255 1103 8 -0.42330619436302186 1103 9 -0.5278552691938216 1103 10-11 -0.5110096237220532 1103 12-13 -0.5927603827201295 1103 14-15 -0.7291179797530702 1103 16-17 -0.7278643627016308 1103 18-19 -0.7084805032211889 1103 20-21 -0.6917636313005957 1103 22-23 -0.7098134828090892 1103 24-25 -0.8314129748541461 1103 26-27 -0.7881311507725215 1103 28-29 -0.8357962024795711 1103 30-31 -0.7099422228361121 1103 32-33 -0.7588530823108641 1103 34-35 -0.8674929684851094 1103 36-37 -0.9122991898552257 1103 38-39 -0.8092123804651621 1103 40-41 -0.8379144443343165 1103 42-43 -0.7052816513265583 1103 44-45 -0.771172581446308 1103 46-47 -0.8268991813647233 1103 48-49 -0.8289503672786509 1103 50-51 -0.8607223679659484 1103 52-53 -0.8752053254088636 1103 54-55 -0.832032785130707 1103 56-57 -0.8951561944712196 1103 58-59 -0.9436716466637378 1103 60-61 -0.9075180879544078 1103 62-63 -0.7992558528611582 1103 64-65 -0.7989912731153979 1103 66-67 -1.001762711772571 1103 68-69 -1.0321435431452244 1103 70-71 -0.9576866244991109 1103 72-73 -0.8426918968296278 1103 74-75 -0.8218079432289471 1103 76-77 -0.8493076537421445 1103 78-79 -0.7811057359738527 1103 80-81 -0.6697671297500847 1103 82-83 -0.8099722110333261 1103 84-85 -0.86741265854792 1103 86-87 -0.7773321017409849 1103 88-89 -0.8589528578770533 1103 90-91 -0.7460048029153228 1103 92-93 -0.8922519190189853 1103 94-95 -0.9907852429594115 1103 96-97 -0.9812617885730788 1103 98-99 -0.9965308371837338 1103 100 -1.2100454316906948 1104 1 -0.36432202499510424 1104 2 -0.36315604930670276 1104 3 -0.4212837506359328 1104 4 -0.27996295650876846 1104 5 -0.29808475957373304 1104 6 -0.39551151689276054 1104 7 -0.32611757323601154 1104 8 -0.4098548664949391 1104 9 -0.6212308614916324 1104 10-11 -0.5473882672076584 1104 12-13 -0.564028855334314 1104 14-15 -0.7429271205520038 1104 16-17 -0.6961182225073657 1104 18-19 -0.7702229940999459 1104 20-21 -0.789646128250638 1104 22-23 -0.7523415502183255 1104 24-25 -0.826402366473971 1104 26-27 -0.9162561226457981 1104 28-29 -0.8966545934914478 1104 30-31 -0.875834296375551 1104 32-33 -0.8370360668454637 1104 34-35 -0.8348077432217025 1104 36-37 -0.7926618221735993 1104 38-39 -0.7951183721339206 1104 40-41 -0.823007072307874 1104 42-43 -0.7376257555955235 1104 44-45 -0.8350313071151518 1104 46-47 -0.7649025066602917 1104 48-49 -0.8743265781599945 1104 50-51 -0.8201882505479929 1104 52-53 -0.8308767380948936 1104 54-55 -0.8639381533065418 1104 56-57 -0.7979086456902635 1104 58-59 -0.7727445405136635 1104 60-61 -0.8759129291641798 1104 62-63 -0.8432933540782841 1104 64-65 -0.8531166361036497 1104 66-67 -0.9305408783924634 1104 68-69 -0.9436656431872024 1104 70-71 -1.0787873595170794 1104 72-73 -0.9868424777335534 1104 74-75 -1.076842403077066 1104 76-77 -0.8632567940665083 1104 78-79 -0.7359646031968623 1104 80-81 -0.7330165179169761 1104 82-83 -0.7561630947813534 1104 84-85 -0.8459818895815054 1104 86-87 -0.829547015331574 1104 88-89 -0.9174221336679373 1104 90-91 -0.8585719081817089 1104 92-93 -0.916131615978518 1104 94-95 -0.9396702788838951 1104 96-97 -1.040285416663643 1104 98-99 -1.0570195099865956 1104 100 -1.0647635629793228 1105 1 -0.4201780299287279 1105 2 -0.3986978393052709 1105 3 -0.43758197027204915 1105 4 -0.29605896280325794 1105 5 -0.31608905445093427 1105 6 -0.3661619913713494 1105 7 -0.4001399152546412 1105 8 -0.4638024630512092 1105 9 -0.5669613767547119 1105 10-11 -0.5576602377630948 1105 12-13 -0.5401607528996806 1105 14-15 -0.8368714099777534 1105 16-17 -0.7152672749038587 1105 18-19 -0.656365162857206 1105 20-21 -0.7638743293609025 1105 22-23 -0.7816157648466984 1105 24-25 -0.7928495068123453 1105 26-27 -0.8679737341101088 1105 28-29 -0.8202762504125616 1105 30-31 -0.8025663211308611 1105 32-33 -0.784656881530438 1105 34-35 -0.801028371873592 1105 36-37 -0.8936316675482772 1105 38-39 -0.8861154739822794 1105 40-41 -1.0136561587659827 1105 42-43 -0.8281661603688093 1105 44-45 -0.8784462400273654 1105 46-47 -0.872990329543768 1105 48-49 -0.9793661666925786 1105 50-51 -0.9427666120113756 1105 52-53 -0.9610863571407791 1105 54-55 -0.9979483369684843 1105 56-57 -0.9850071789327544 1105 58-59 -1.155569236480865 1105 60-61 -0.9900406177850272 1105 62-63 -1.092605752846822 1105 64-65 -1.0322921718991225 1105 66-67 -1.107655746831199 1105 68-69 -1.0766716084009218 1105 70-71 -1.0889584430251986 1105 72-73 -1.0676015907708987 1105 74-75 -1.1232529405644414 1105 76-77 -1.0641755749820092 1105 78-79 -0.9950380683740399 1105 80-81 -0.959702266005312 1105 82-83 -0.7987470123198364 1105 84-85 -0.7959826442876974 1105 86-87 -0.9254899384596023 1105 88-89 -0.987373474685004 1105 90-91 -0.9724205951007505 1105 92-93 -0.996848321715536 1105 94-95 -0.9003096156560773 1105 96-97 -1.0397356191924416 1105 98-99 -1.0295069353435586 1105 100 -1.0342018355598732 1106 1 -0.4033688612969968 1106 2 -0.3752198980194841 1106 3 -0.4004019436154742 1106 4 -0.3010715946087359 1106 5 -0.32041599161576784 1106 6 -0.35089700863944273 1106 7 -0.38693711600434 1106 8 -0.4064791756412376 1106 9 -0.5373862057733376 1106 10-11 -0.4551790156167428 1106 12-13 -0.599120194807 1106 14-15 -0.7132067541982323 1106 16-17 -0.6947754592460385 1106 18-19 -0.7248760604397262 1106 20-21 -0.7418589221789134 1106 22-23 -0.6929395018809217 1106 24-25 -0.7060161070343653 1106 26-27 -0.6623209271182802 1106 28-29 -0.7248682156169224 1106 30-31 -0.743495885345645 1106 32-33 -0.8034400701022975 1106 34-35 -0.8007224571336025 1106 36-37 -0.760062627336012 1106 38-39 -0.6479975363085515 1106 40-41 -0.6652548883793656 1106 42-43 -0.6694460917930769 1106 44-45 -0.7543524955277761 1106 46-47 -0.8365519946159026 1106 48-49 -0.834902309488406 1106 50-51 -0.8675809399853165 1106 52-53 -0.887752433304577 1106 54-55 -0.8667659887569883 1106 56-57 -0.873035970419096 1106 58-59 -0.9331850683698804 1106 60-61 -0.9557630839775939 1106 62-63 -1.0052519037756653 1106 64-65 -0.8966438058392399 1106 66-67 -0.9863837848419124 1106 68-69 -0.9062993989927506 1106 70-71 -0.9392587109835233 1106 72-73 -0.9110333010176745 1106 74-75 -0.9328280660420489 1106 76-77 -0.9605739011182308 1106 78-79 -0.7998386526423609 1106 80-81 -0.6959820281634208 1106 82-83 -0.7922925780820549 1106 84-85 -0.7116540109308573 1106 86-87 -0.7022672850543508 1106 88-89 -0.8043992922258205 1106 90-91 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0.9816351216669226 2203 90-91 0.9757005456837504 2203 92-93 1.0218949167704885 2203 94-95 1.0867589478421529 2203 96-97 1.1014139624272552 2203 98-99 1.0175563789294557 2203 100 1.3742602448664165 2204 1 0.7084335903639456 2204 2 0.6561701904994166 2204 3 0.6529852843359123 2204 4 0.4527620469511646 2204 5 0.502128499830917 2204 6 0.5230487755575766 2204 7 0.521657195283133 2204 8 0.49803496223373855 2204 9 0.679693384170946 2204 10-11 0.7026613357136569 2204 12-13 0.7339839541559172 2204 14-15 0.912518106449987 2204 16-17 0.9337667617135352 2204 18-19 0.8755797698305301 2204 20-21 0.9358899165278061 2204 22-23 0.9070793542432014 2204 24-25 0.8553458705831645 2204 26-27 0.9512985070475111 2204 28-29 1.0071472714855503 2204 30-31 1.0494556635356105 2204 32-33 1.0614023330571314 2204 34-35 1.11458242268084 2204 36-37 1.1242509470440964 2204 38-39 1.1702616900249012 2204 40-41 1.1695013945062769 2204 42-43 -0.5822394817981404 2204 44-45 0.7160480637261699 2204 46-47 0.9961855201560539 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1.2873719290593684 2206 100 1.4727863759083988 2207 1 0.6573812719606309 2207 2 0.6152534791147275 2207 3 0.6082154582737402 2207 4 0.369664037149775 2207 5 0.45435785405271645 2207 6 0.49405632975189206 2207 7 0.5021620714513446 2207 8 0.568123116344843 2207 9 0.6770938096443757 2207 10-11 0.6780667656114616 2207 12-13 0.6828213541769657 2207 14-15 0.8901912363534521 2207 16-17 0.9423229455890549 2207 18-19 0.9434590158116194 2207 20-21 0.931949082125044 2207 22-23 0.9965422399057289 2207 24-25 0.9752010665679407 2207 26-27 1.0954968161632124 2207 28-29 0.9467403043861609 2207 30-31 0.9962582842302723 2207 32-33 1.0019577552595678 2207 34-35 1.0471604052357577 2207 36-37 1.12704739945994 2207 38-39 1.090477089227356 2207 40-41 1.1117129126959995 2207 42-43 1.1859119480989122 2207 44-45 1.250794904786268 2207 46-47 1.181570253066809 2207 48-49 1.1409376649116822 2207 50-51 1.1492450641408212 2207 52-53 1.1092088188522524 2207 54-55 1.1340010606595925 2207 56-57 1.1662104887192228 2207 58-59 1.1772154356847082 2207 60-61 1.1311320606605264 2207 62-63 1.22479623596589 2207 64-65 1.2588749556162924 2207 66-67 1.3067498252821466 2207 68-69 1.2398736134149928 2207 70-71 1.2130538561248372 2207 72-73 1.332244917893128 2207 74-75 1.238124208093602 2207 76-77 1.2449675852199888 2207 78-79 1.114311953527526 2207 80-81 1.076595154068471 2207 82-83 1.0546045614712938 2207 84-85 1.1198878103003693 2207 86-87 1.1093424219684245 2207 88-89 1.1603683385144699 2207 90-91 1.0800785217820845 2207 92-93 1.1286038429700476 2207 94-95 1.1976209104846092 2207 96-97 1.2770292947285213 2207 98-99 1.1919782212978802 2207 100 1.417272720454303 2208 1 0.6469534652227438 2208 2 0.6099711007148372 2208 3 0.6099283907607926 2208 4 0.27331179609568323 2208 5 0.4235018480078878 2208 6 0.5286690049133682 2208 7 0.5333291210973812 2208 8 0.5408987966345009 2208 9 0.7045679364120545 2208 10-11 0.694296946224398 2208 12-13 0.7044276568808812 2208 14-15 0.9350009056195816 2208 16-17 0.937606952384364 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1.0786671889813988 2208 86-87 1.065578304607083 2208 88-89 1.158042406560579 2208 90-91 1.2137818448341307 2208 92-93 1.1694747317669645 2208 94-95 1.235886538338562 2208 96-97 1.2660417336722745 2208 98-99 1.3788426576968575 2208 100 1.5641405663391978 2209 1 0.6556635755686173 2209 2 0.600305849744835 2209 3 0.5814599501924107 2209 4 0.396358748278999 2209 5 0.4976940622928083 2209 6 0.348530456190673 2209 7 0.4416687651905562 2209 8 0.506568809415235 2209 9 0.734175994974656 2209 10-11 0.714850316417305 2209 12-13 0.7131352306954355 2209 14-15 0.9464859812286406 2209 16-17 0.9314475779330493 2209 18-19 0.944966960111806 2209 20-21 0.9398592687586529 2209 22-23 0.9999726010380598 2209 24-25 0.97696619866079 2209 26-27 1.0194496316799686 2209 28-29 0.979476256392644 2209 30-31 1.0039990371624654 2209 32-33 1.0317794163167662 2209 34-35 1.0182993594854253 2209 36-37 1.040341025848484 2209 38-39 1.0538114042107338 2209 40-41 1.0786110318645967 2209 42-43 1.1825844620836463 2209 44-45 1.203978735842064 2209 46-47 1.1860911921158817 2209 48-49 1.2173245119551552 2209 50-51 1.2154659151698795 2209 52-53 1.230172528233858 2209 54-55 1.2594222529232795 2209 56-57 1.2571719450679737 2209 58-59 1.1921816534880705 2209 60-61 1.2265325027054743 2209 62-63 1.2467988797468976 2209 64-65 1.2621882330814316 2209 66-67 1.2142111546924994 2209 68-69 1.2408430667007408 2209 70-71 1.2171578980636824 2209 72-73 1.2835365089915953 2209 74-75 1.2453792241419634 2209 76-77 1.2647620508515374 2209 78-79 1.0704952225504414 2209 80-81 1.01989683422822 2209 82-83 1.0363403045469184 2209 84-85 1.0604558696986714 2209 86-87 1.1021245958223709 2209 88-89 1.1496585851973258 2209 90-91 1.1826198161304262 2209 92-93 1.2368737379513952 2209 94-95 1.2470263334997327 2209 96-97 1.2639288681226688 2209 98-99 1.3134061983137144 2209 100 1.510072333022336 2210 1 0.6891505791591612 2210 2 0.6255838673408434 2210 3 0.6086611430590239 2210 4 0.3349555218615947 2210 5 0.45228708118690264 2210 6 0.46366145808852366 2210 7 0.5411567813824192 2210 8 0.5773362226558305 2210 9 0.700758804932903 2210 10-11 0.7007928070745919 2210 12-13 0.7384895147665134 2210 14-15 0.8873948713209998 2210 16-17 0.9382813538803774 2210 18-19 0.850086879489858 2210 20-21 0.9334314174410849 2210 22-23 0.9617777431412833 2210 24-25 0.9666359909911364 2210 26-27 1.004321952785098 2210 28-29 1.0266959381632859 2210 30-31 0.9794464148570938 2210 32-33 1.0353539563927114 2210 34-35 1.0909486645116147 2210 36-37 1.1475757165159095 2210 38-39 1.1564869246309826 2210 40-41 1.1588980737763706 2210 42-43 1.1946817172349142 2210 44-45 1.167922184631884 2210 46-47 1.1607907417092491 2210 48-49 1.2031685700815942 2210 50-51 1.1878667749130685 2210 52-53 1.2669107142405736 2210 54-55 1.208286673817483 2210 56-57 1.2426337816363358 2210 58-59 1.2779031771499731 2210 60-61 1.237389868722083 2210 62-63 1.3145274093051214 2210 64-65 1.3515141860624098 2210 66-67 1.3334078431117078 2210 68-69 1.3823070126138362 2210 70-71 1.3144270095036106 2210 72-73 1.3348322553165985 2210 74-75 1.3067703195660911 2210 76-77 1.31182228945179 2210 78-79 1.20500384123374 2210 80-81 1.105739832956921 2210 82-83 1.1321684318210785 2210 84-85 1.1918917268366869 2210 86-87 1.147859393204424 2210 88-89 1.161778349374881 2210 90-91 1.2625751547671626 2210 92-93 1.3249123702490913 2210 94-95 1.3737153393164405 2210 96-97 1.3855380998923934 2210 98-99 1.4681132637878207 2210 100 1.5340905310873865 2211 1 0.6820115746719679 2211 2 0.6209732286987517 2211 3 0.5978630723338014 2211 4 0.3765370691889913 2211 5 0.4593618540398623 2211 6 0.49998950377930385 2211 7 0.5270689079844857 2211 8 0.511156792070139 2211 9 0.6801930283477233 2211 10-11 0.6871200290598978 2211 12-13 0.7384336444262516 2211 14-15 0.9294959867225785 2211 16-17 0.8703573324741782 2211 18-19 0.8517873654164632 2211 20-21 0.9123660380111573 2211 22-23 0.9516038746920898 2211 24-25 0.9152266433328435 2211 26-27 0.9882195169202603 2211 28-29 0.9062404536302751 2211 30-31 1.008948687774847 2211 32-33 1.0406581600214508 2211 34-35 1.0525033727065534 2211 36-37 1.0342198402395013 2211 38-39 1.0785045771737174 2211 40-41 1.065406234163234 2211 42-43 1.024441286922226 2211 44-45 1.054773439845981 2211 46-47 1.150294124827937 2211 48-49 1.1279412255045642 2211 50-51 1.2020940943192358 2211 52-53 1.212174217624728 2211 54-55 1.2185606665878126 2211 56-57 1.1769244595240806 2211 58-59 1.1980214120587007 2211 60-61 1.1725009721800532 2211 62-63 1.1788412997848994 2211 64-65 1.2921470358793883 2211 66-67 1.305082947230936 2211 68-69 1.2775377876819363 2211 70-71 1.2224759588293708 2211 72-73 1.3027082881134433 2211 74-75 1.3338119990883968 2211 76-77 1.300450979344859 2211 78-79 1.1431836016991852 2211 80-81 1.036977967653371 2211 82-83 1.0302980289953112 2211 84-85 1.0478194755164125 2211 86-87 1.1032166432178236 2211 88-89 1.1716521678824172 2211 90-91 1.215601169175379 2211 92-93 1.201055640536783 2211 94-95 1.257502174077672 2211 96-97 1.2495859074107898 2211 98-99 1.3158501124725248 2211 100 1.4675587831547823 2212 1 0.20904267204398508 2212 2 0.3765059839531304 2212 3 0.47288781684670766 2212 4 0.32828460622622657 2212 5 0.40329233433085676 2212 6 0.49636634739280083 2212 7 0.525657955238259 2212 8 0.5166536825096841 2212 9 0.6923811738407437 2212 10-11 0.67448666644556 2212 12-13 0.721824221538995 2212 14-15 0.9130837466725268 2212 16-17 0.5360922135767936 2212 18-19 0.7302423180307258 2212 20-21 0.873912475802868 2212 22-23 0.9252510420934286 2212 24-25 0.9754654661774396 2212 26-27 0.9818338860589009 2212 28-29 0.9749359715152579 2212 30-31 1.0266051865087533 2212 32-33 1.0164567376243667 2212 34-35 0.8945156598300983 2212 36-37 0.9073987619207671 2212 38-39 0.9590685208245731 2212 40-41 1.006642434678632 2212 42-43 0.993566762812236 2212 44-45 0.9566726401268895 2212 46-47 1.0513165150771897 2212 48-49 1.0825693994302128 2212 50-51 1.0779115837998745 2212 52-53 1.080167322742959 2212 54-55 1.077178694379434 2212 56-57 1.0569056919893498 2212 58-59 1.1341953712868147 2212 60-61 1.153059922741697 2212 62-63 1.1810780638271083 2212 64-65 1.227620673841237 2212 66-67 1.2143823034022816 2212 68-69 1.160375501677784 2212 70-71 1.186239239857521 2212 72-73 1.2025342517060196 2212 74-75 1.1597750393288706 2212 76-77 1.173805911103024 2212 78-79 0.9993798339346895 2212 80-81 0.9805890154303114 2212 82-83 0.8810704628420325 2212 84-85 0.994798428246142 2212 86-87 1.0118296337336083 2212 88-89 0.9989618325000045 2212 90-91 1.0433815686932135 2212 92-93 1.1165697357772295 2212 94-95 1.167748928422391 2212 96-97 1.2352598654605913 2212 98-99 1.2190319033389727 2212 100 1.3026599499174836 2213 1 0.4390905310532105 2213 2 0.4623562479108969 2213 3 0.5094859611436249 2213 4 0.2746778499738909 2213 5 0.3674360713329392 2213 6 0.41686306791751804 2213 7 0.472991048609984 2213 8 0.4998770334401996 2213 9 0.6496973365880407 2213 10-11 0.6548188133020574 2213 12-13 0.6024469885629316 2213 14-15 0.8139756039753578 2213 16-17 0.8096554434600094 2213 18-19 0.8176549986684805 2213 20-21 0.8630488806447545 2213 22-23 0.9418997116396426 2213 24-25 0.7888013324388368 2213 26-27 0.9185180165980213 2213 28-29 0.9628455726457474 2213 30-31 0.9356000806590217 2213 32-33 0.9249097184807624 2213 34-35 0.9881356806267689 2213 36-37 1.0522275893083801 2213 38-39 1.0311146601089547 2213 40-41 1.0030470099465845 2213 42-43 1.1066353385067913 2213 44-45 1.110557351906344 2213 46-47 0.9972171709722488 2213 48-49 1.0294562308705153 2213 50-51 0.9167117091131516 2213 52-53 1.038862598165558 2213 54-55 1.1088636247905654 2213 56-57 1.1341046734856448 2213 58-59 1.170127622126678 2213 60-61 1.1609649912640734 2213 62-63 1.1832040570015394 2213 64-65 1.1313150827358172 2213 66-67 1.1538899821736734 2213 68-69 1.1745822286014729 2213 70-71 1.1513408236041158 2213 72-73 1.1697448313466126 2213 74-75 1.2305780441814278 2213 76-77 1.0925522450229366 2213 78-79 0.997281503000977 2213 80-81 0.9712268467106355 2213 82-83 0.7684648130793263 2213 84-85 0.6310876219751407 2213 86-87 0.8333575226047181 2213 88-89 0.8146326585011785 2213 90-91 0.9705892154697295 2213 92-93 1.13191961313019 2213 94-95 1.187190151160081 2213 96-97 1.197811190581902 2213 98-99 1.205875341722166 2213 100 1.3501770138539193 2214 1 0.4460320758891001 2214 2 0.46752512079803665 2214 3 0.4785715859152333 2214 4 0.32685848150155294 2214 5 0.43155687382498087 2214 6 0.4312609581041116 2214 7 0.4924086215125172 2214 8 0.4957406415195109 2214 9 0.6101982000969741 2214 10-11 0.6255517855574055 2214 12-13 0.643581740335982 2214 14-15 0.8902385979199394 2214 16-17 0.7978158814370246 2214 18-19 0.8038799302345794 2214 20-21 0.8557081812827789 2214 22-23 0.9169668472166776 2214 24-25 0.9188346358397155 2214 26-27 0.9840176714777158 2214 28-29 0.8778141624492548 2214 30-31 0.7931460581426322 2214 32-33 0.9120412400033331 2214 34-35 0.9403912849366662 2214 36-37 1.00557903319541 2214 38-39 0.8897633701333021 2214 40-41 0.8159011359518473 2214 42-43 0.8599560001179896 2214 44-45 0.8747898109663836 2214 46-47 0.961013579266087 2214 48-49 0.9994974517371205 2214 50-51 0.9622149709957242 2214 52-53 0.9670812816850471 2214 54-55 0.8633156058566342 2214 56-57 0.978369562523099 2214 58-59 0.9587633441079717 2214 60-61 0.9569648534047985 2214 62-63 0.9521886951351135 2214 64-65 0.96288490326382 2214 66-67 0.9731281019620539 2214 68-69 1.066708858952616 2214 70-71 1.0465099656001158 2214 72-73 1.0452976139793648 2214 74-75 1.0737177041222594 2214 76-77 0.9924298241008032 2214 78-79 0.8057617603256872 2214 80-81 0.7825892834582504 2214 82-83 0.8444939183820921 2214 84-85 0.851059464690934 2214 86-87 0.9577710541532447 2214 88-89 0.9390260540978055 2214 90-91 0.95448767739461 2214 92-93 1.0414094299567687 2214 94-95 1.1410514618996075 2214 96-97 1.1475058228517376 2214 98-99 1.2335789886664585 2214 100 1.354081169555581 2215 1 0.6367485107147317 2215 2 0.5503862865615048 2215 3 0.5665687785101241 2215 4 0.36119094615010994 2215 5 0.423207863895513 2215 6 0.4389867873816655 2215 7 0.47826516771389294 2215 8 0.47057668445900447 2215 9 0.6113197239196495 2215 10-11 0.6302533980894793 2215 12-13 0.6779181600890709 2215 14-15 0.8279374318113 2215 16-17 0.9296472818029073 2215 18-19 0.899374059421234 2215 20-21 0.8847288686367989 2215 22-23 0.9345816603056321 2215 24-25 0.9431021504807973 2215 26-27 0.9401063032129002 2215 28-29 0.9008735005229624 2215 30-31 0.684678610810245 2215 32-33 0.7523769780402176 2215 34-35 0.8980145691404147 2215 36-37 0.9656781409238278 2215 38-39 0.9212001229780995 2215 40-41 0.901766239561212 2215 42-43 0.906015709252415 2215 44-45 0.8084629956453213 2215 46-47 0.8605856369559604 2215 48-49 0.8355687960699569 2215 50-51 0.8403999807180966 2215 52-53 0.8662395635301081 2215 54-55 0.907183683704595 2215 56-57 0.9654130715493636 2215 58-59 0.9399412173835415 2215 60-61 0.9661452982174268 2215 62-63 0.9820393791524609 2215 64-65 1.0213294829009314 2215 66-67 0.9242826329298097 2215 68-69 0.9436160103568838 2215 70-71 0.9352750237617187 2215 72-73 0.8223279489119975 2215 74-75 0.9389486678446062 2215 76-77 0.8830790940604274 2215 78-79 0.894352218600055 2215 80-81 0.8341888715521009 2215 82-83 0.8632347856861813 2215 84-85 0.7137839708062472 2215 86-87 0.8234814484555919 2215 88-89 0.8594579802768543 2215 90-91 0.939853071524368 2215 92-93 1.020528707280068 2215 94-95 1.016839246810953 2215 96-97 1.0617624645509096 2215 98-99 1.0130128046663671 2215 100 1.027656468992948 2216 1 0.45223426748055573 2216 2 0.09164610698799436 2216 3 0.3567049628043861 2216 4 0.2280981337188095 2216 5 0.35085908978556546 2216 6 0.3675447033107133 2216 7 0.4316217576684167 2216 8 0.4495580791620455 2216 9 0.6284379277216985 2216 10-11 0.6080729018325286 2216 12-13 0.631319947146352 2216 14-15 0.8133713095346948 2216 16-17 0.824021869605339 2216 18-19 0.8113148447082423 2216 20-21 0.8448226472013687 2216 22-23 0.8794927557966261 2216 24-25 0.8799177578720077 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40-41 1.0332377660556773 2311 42-43 1.0469859255260587 2311 44-45 1.080140521844747 2311 46-47 0.7384385568196876 2311 48-49 0.9338997098056936 2311 50-51 1.0594252566668487 2311 52-53 0.9724745126861691 2311 54-55 0.9851257125384265 2311 56-57 1.08400044020334 2311 58-59 1.084829287945091 2311 60-61 1.0547869944375705 2311 62-63 1.0473462618734928 2311 64-65 1.0752905585279322 2311 66-67 1.128657257904642 2311 68-69 1.1614248840753234 2311 70-71 1.1739006973119004 2311 72-73 1.1958982931676516 2311 74-75 1.2044598707558904 2311 76-77 1.1412872861052037 2311 78-79 0.8513289077333468 2311 80-81 0.8350063516620594 2311 82-83 0.9012669227266059 2311 84-85 0.979540061414319 2311 86-87 1.0596705797527797 2311 88-89 1.056444621905296 2311 90-91 1.1157081039883394 2311 92-93 1.1262565127673447 2311 94-95 1.1385575274065332 2311 96-97 1.255452796925372 2311 98-99 1.3179729423566684 2311 100 1.4214992783774996 2312 1 0.21728145468885884 2312 2 0.3093498352255395 2312 3 0.45805939262601214 2312 4 0.1650144545679737 2312 5 0.33445828679319334 2312 6 0.4435375087033364 2312 7 0.32888059384809054 2312 8 0.4246017415135199 2312 9 0.6222691561122815 2312 10-11 0.604977060119424 2312 12-13 0.6916703405788667 2312 14-15 0.8943964752705185 2312 16-17 0.8100056399234248 2312 18-19 0.7828641704599875 2312 20-21 0.8106527900765883 2312 22-23 0.9102120834184291 2312 24-25 0.9334221202472719 2312 26-27 0.9677896623601043 2312 28-29 0.9929372876719569 2312 30-31 0.952615302782327 2312 32-33 0.8927162348745412 2312 34-35 0.9927516441160122 2312 36-37 1.0572174616083672 2312 38-39 0.817040154228529 2312 40-41 1.0066951365267087 2312 42-43 1.0946932226335804 2312 44-45 0.8227249878503144 2312 46-47 0.935571495550704 2312 48-49 1.0725240525170747 2312 50-51 0.8768455598972551 2312 52-53 1.0626825417662857 2312 54-55 0.8786330420734672 2312 56-57 0.9671239004679464 2312 58-59 1.0348475492130191 2312 60-61 1.057980292829761 2312 62-63 1.128442328778256 2312 64-65 1.1423855953125113 2312 66-67 1.1451081805456411 2312 68-69 1.168453585867347 2312 70-71 1.0634018021320415 2312 72-73 1.0551372069001204 2312 74-75 1.1359416086311072 2312 76-77 1.157129994319149 2312 78-79 1.0545274546894134 2312 80-81 0.9429191909205201 2312 82-83 0.959041742915332 2312 84-85 0.969519294545421 2312 86-87 1.0252398472525108 2312 88-89 0.9516799873834891 2312 90-91 1.1172113494654354 2312 92-93 1.1644562998628807 2312 94-95 1.2403133561835418 2312 96-97 0.9133805493181804 2312 98-99 0.6675289974920666 2312 100 0.8729425042758159 2313 1 0.11032266669965196 2313 2 0.2702100210865481 2313 3 0.2962530788676787 2313 4 0.18724671505484736 2313 5 0.28722888164730875 2313 6 0.2690568783027061 2313 7 0.23674579770354853 2313 8 0.3426238936802619 2313 9 0.5946564384565391 2313 10-11 0.35290521174802336 2313 12-13 0.392398795317213 2313 14-15 0.43759278001812163 2313 16-17 0.6996865561490111 2313 18-19 0.6671780843868973 2313 20-21 0.6253859364951779 2313 22-23 0.5889978812249055 2313 24-25 0.46807160059232444 2313 26-27 0.6646795929429956 2313 28-29 0.5516253539939839 2313 30-31 0.7390268028664195 2313 32-33 0.8402936042276892 2313 34-35 0.933547661122482 2313 36-37 0.933254125139797 2313 38-39 0.8916518089128687 2313 40-41 0.8632867263211779 2313 42-43 0.986828974428299 2313 44-45 0.8721086998023893 2313 46-47 0.8014381836213289 2313 48-49 0.90151661171722 2313 50-51 0.8475132829443552 2313 52-53 0.9300828895105226 2313 54-55 0.9246887817023506 2313 56-57 0.622201361536618 2313 58-59 0.6995363825481888 2313 60-61 0.5194810698271297 2313 62-63 0.8683928177888518 2313 64-65 0.9483148327650142 2313 66-67 0.8772533417062718 2313 68-69 0.9983162999974482 2313 70-71 0.9658786543171303 2313 72-73 0.9947257317949152 2313 74-75 1.09356126049434 2313 76-77 1.0594536247398452 2313 78-79 0.8823274920013233 2313 80-81 0.7537548488342196 2313 82-83 0.8688580129354406 2313 84-85 0.8651245942049357 2313 86-87 0.8084335925196307 2313 88-89 0.9249818953867326 2313 90-91 0.7555130681717372 2313 92-93 0.9385034830253112 2313 94-95 1.0720898392359146 2313 96-97 1.1216609379613125 2313 98-99 1.1231717855239935 2313 100 1.2217934620267172 2314 1 -0.44193086473915244 2314 2 0.03812739041045177 2314 3 0.24738214089211397 2314 4 0.18596255108838022 2314 5 0.28491410935665584 2314 6 0.3958734976213165 2314 7 0.4442477385199908 2314 8 0.5001474905592218 2314 9 0.42942938259793095 2314 10-11 0.5735988955477183 2314 12-13 0.4570327207281437 2314 14-15 0.7657393562847901 2314 16-17 0.762968632565439 2314 18-19 0.847220646204093 2314 20-21 0.8979307567994681 2314 22-23 0.9213251243579137 2314 24-25 0.8388926370457881 2314 26-27 0.744538220424225 2314 28-29 0.8115574148294229 2314 30-31 0.8555466209772788 2314 32-33 0.9022273774592477 2314 34-35 0.8530817441585725 2314 36-37 0.8013965505098852 2314 38-39 0.4667335289330765 2314 40-41 0.5296879897427544 2314 42-43 0.7297284906612731 2314 44-45 0.7416049709539649 2314 46-47 0.6413131242471763 2314 48-49 0.689401221632771 2314 50-51 0.6811626895053138 2314 52-53 0.6838548503855932 2314 54-55 0.5851747875535409 2314 56-57 0.634869498564619 2314 58-59 0.7400497867372096 2314 60-61 0.8288497281375413 2314 62-63 0.8173418482882653 2314 64-65 0.7379309716445661 2314 66-67 0.4546618994512315 2314 68-69 0.4565068963409118 2314 70-71 0.5906595553521399 2314 72-73 0.6846725385997203 2314 74-75 0.7379067288467809 2314 76-77 0.6271967411192421 2314 78-79 0.5030403086508883 2314 80-81 0.5591699899253655 2314 82-83 0.6477627450180634 2314 84-85 0.6439720608570809 2314 86-87 0.7018565392326472 2314 88-89 0.5784616113589145 2314 90-91 0.7424874215606749 2314 92-93 0.6676777631987285 2314 94-95 0.7060464000426165 2314 96-97 0.6978862844492362 2314 98-99 0.8295246056830692 2314 100 0.7719396933938185 2315 1 0.4012787827336197 2315 2 0.4236011745841637 2315 3 0.46594938648019735 2315 4 0.31270248692611347 2315 5 0.37859932464115786 2315 6 0.42641276505135295 2315 7 0.39908283418508006 2315 8 0.29857379830678354 2315 9 0.5383300650068819 2315 10-11 0.5613705411820717 2315 12-13 0.3830777560660863 2315 14-15 0.628356580262242 2315 16-17 0.771663362615179 2315 18-19 0.7690201847714633 2315 20-21 0.49912791271865586 2315 22-23 0.8140728034978721 2315 24-25 0.8630142531557539 2315 26-27 0.8274921075984736 2315 28-29 0.7895160076425185 2315 30-31 0.8601266016190223 2315 32-33 0.8112979863920486 2315 34-35 0.8988654186963743 2315 36-37 0.8703384574806776 2315 38-39 0.9260277683301794 2315 40-41 0.8688816636109422 2315 42-43 0.9828506485588946 2315 44-45 0.9954333886296283 2315 46-47 0.9211594513529917 2315 48-49 0.8192701090200813 2315 50-51 0.9382966206313199 2315 52-53 0.9910134480686921 2315 54-55 0.8082050241747325 2315 56-57 0.7974518046320256 2315 58-59 0.7711926786657912 2315 60-61 0.909885260885261 2315 62-63 0.9505503756607183 2315 64-65 1.039631563958487 2315 66-67 1.0330655860422908 2315 68-69 1.0549202742347603 2315 70-71 0.8683701920439475 2315 72-73 1.0424798935193351 2315 74-75 0.48386017163339545 2315 76-77 0.4856611079277471 2315 78-79 0.6803543324286707 2315 80-81 0.7468914867203011 2315 82-83 0.7038313146377746 2315 84-85 0.7385641556273086 2315 86-87 0.7788455999919996 2315 88-89 0.813261338638835 2315 90-91 0.908789325122477 2315 92-93 0.9116352237365 2315 94-95 0.972597489401057 2315 96-97 1.1330638873127974 2315 98-99 1.1341345855334382 2315 100 1.0298808759519105 2316 1 0.28832456783425897 2316 2 0.12142642320603159 2316 3 0.18699668650463508 2316 4 -0.011408762938103223 2316 5 0.23346980661577987 2316 6 0.34875641220509124 2316 7 0.3936335745762065 2316 8 0.4297771850070262 2316 9 0.5917209340658047 2316 10-11 0.562595367439215 2316 12-13 0.35027319995512585 2316 14-15 0.45424124756195994 2316 16-17 0.6908356877274358 2316 18-19 0.6372756871791765 2316 20-21 0.5975075451346825 2316 22-23 0.603007681476349 2316 24-25 0.373368332815474 2316 26-27 0.615928742007128 2316 28-29 0.7648290729783582 2316 30-31 0.44197693850704667 2316 32-33 0.5515837598602786 2316 34-35 0.6854516587330366 2316 36-37 0.7967506925465031 2316 38-39 0.6676667120813207 2316 40-41 0.6997725340209868 2316 42-43 0.7797498082561987 2316 44-45 0.5859535493155121 2316 46-47 0.7417975146167066 2316 48-49 0.8050806323114372 2316 50-51 0.8219933992540049 2316 52-53 0.9097205091985714 2316 54-55 0.8164198033633099 2316 56-57 0.8861552629415783 2316 58-59 0.7429577176893005 2316 60-61 0.8948949636395582 2316 62-63 0.9010109942501785 2316 64-65 0.8753379577338265 2316 66-67 0.8308011744047619 2316 68-69 0.8902808429507374 2316 70-71 0.8366703599904994 2316 72-73 0.8990940560277139 2316 74-75 0.8258582600210431 2316 76-77 0.7546700630956096 2316 78-79 0.44524519959603737 2316 80-81 0.47735347018564767 2316 82-83 0.38119556046105885 2316 84-85 0.5069456388307572 2316 86-87 0.5906202118167307 2316 88-89 0.5835694684596611 2316 90-91 0.4090598191539918 2316 92-93 0.2975475427927954 2316 94-95 0.3075128643938285 2316 96-97 0.6003725304114624 2316 98-99 0.8016823496795453 2316 100 0.4564429624020079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 2.0 12 20.0 13 107.0 14 447.0 15 1357.0 16 3177.0 17 6661.0 18 11111.0 19 16581.0 20 23065.0 21 31718.0 22 39215.0 23 41562.0 24 42473.0 25 45423.0 26 53524.0 27 64885.0 28 80216.0 29 99643.0 30 123034.0 31 152824.0 32 194838.0 33 256970.0 34 364660.0 35 511451.0 36 795890.0 37 1217257.0 38 1065116.0 39 170314.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54615444320832 20.06804430826548 13.642421800790217 23.743379447735986 2 15.530224103916831 20.049376011349278 40.90565173379439 23.514748150939493 3 18.98019048463488 25.422162126216392 29.67603573249396 25.92161165665477 4 12.630129630242164 16.31617723555392 38.20053614229964 32.85315699190428 5 13.121328663578128 37.62644174997599 34.638389525561735 14.61384006088415 6 31.050935059369433 36.96779937785856 17.80815212170919 14.173113441062812 7 27.693012481735508 32.15414767867797 22.130650908560252 18.022188931026264 8 26.140765041857854 36.129940371954866 19.633974685789017 18.09531990039826 9 26.355705813889042 15.961739548116535 20.799903933196624 36.8826507047978 10-11 25.635423585090294 26.284029408143606 27.54109168418526 20.53945532258084 12-13 25.54882102513337 23.771579842392036 28.154186552691122 22.525412579783467 14-15 22.81824974531523 25.281316990406488 25.9377710210758 25.962662243202477 16-17 22.10825195645775 27.960349098322148 26.902946155537776 23.028452789682326 18-19 21.688879406527313 27.417337205092394 29.102596073563426 21.791187314816867 20-21 22.77357859899362 26.70878129422634 28.7841998936559 21.73344021312414 22-23 22.714175145375687 26.514179209244933 28.598897952548786 22.172747692830598 24-25 22.258329294839772 26.4024840730034 29.155734563880102 22.183452068276726 26-27 22.22799890016321 26.70493867172264 28.597308170703016 22.46975425741113 28-29 21.909964634115816 27.126808921427813 28.83414498488561 22.129081459570756 30-31 22.67259341523899 26.683207031557753 28.76975171072428 21.87444784247898 32-33 22.685375195477096 26.81521309486167 28.357820566504614 22.14159114315662 34-35 22.608045100202297 26.841861512290965 28.670763700737595 21.879329686769147 36-37 22.48165320360306 26.736349015233994 28.471025772361507 22.310972008801436 38-39 22.45541556174614 26.842333056342977 28.73918271062853 21.96306867128235 40-41 22.432776492870264 26.263023138772134 29.05686803308661 22.247332335270993 42-43 22.342807357787805 26.459915953588 28.553179411723146 22.644097276901054 44-45 22.19979635671955 27.07235396058284 28.526676983385883 22.201172699311726 46-47 22.246327623909668 26.79836868682763 28.104725442913274 22.850578246349425 48-49 22.645129397994893 26.811863798117848 28.092118485765628 22.45088831812163 50-51 22.502239774007982 26.883735615127062 27.964692718893975 22.64933189197098 52-53 22.235816211603638 27.150777725526364 27.784251472607775 22.829154590262227 54-55 22.19522243190243 27.02595877890377 27.92719570737511 22.851623081818694 56-57 22.69149750814463 26.878353918921576 27.579743851362228 22.850404721571564 58-59 22.125410210993103 27.109700081358667 28.03617098473546 22.728718722912774 60-61 22.393634560835867 27.432561868510767 27.614022513090458 22.55978105756291 62-63 22.515987623128876 27.098184173243684 27.483776853891428 22.902051349736016 64-65 22.07715903946189 26.998161340096644 27.61620977353993 23.308469846901534 66-67 22.227704641037473 26.883103812624377 27.56814944890909 23.32104209742906 68-69 21.948934581443037 26.972154116719636 27.780785407134335 23.298125894702988 70-71 22.05419648596489 26.67297028857552 27.81326850804876 23.45956471741082 72-73 22.250406368239158 26.965737920460953 27.67543506650893 23.10842064479096 74-75 22.400095646592494 27.171642854958396 27.314364349765324 23.113897148683794 76-77 22.22688075638991 26.995486909005706 27.297853288325612 23.47977904627877 78-79 22.05941527321958 26.994174458811436 27.4354593885953 23.51095087937368 80-81 21.878103931709948 27.33652572449934 27.483731865003342 23.30163847878737 82-83 22.440065546414928 26.843169530433947 27.924010878819978 22.792754044331147 84-85 22.50585738927423 27.11505951092717 27.229739957236482 23.149343142562117 86-87 21.83708036396012 27.431888707118713 27.51234526713777 23.218685661783393 88-89 22.14266687690548 26.849351380598 27.448707613571937 23.559274128924578 90-91 22.197426458459873 26.78870248326956 27.5418622567293 23.472008801541268 92-93 22.24688834080539 26.964362058006813 27.42247058352844 23.366279017659352 94-95 21.66792340657527 26.947200943243114 27.679389345413558 23.705486304768066 96-97 21.75985654739869 26.919677922530866 27.497471069702417 23.822994460368026 98-99 21.444327735944402 27.158810090635388 27.3810150683299 24.01584710509031 100 21.66913104102786 26.956775452687893 27.201833184091168 24.17226032219308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1255.0 1 898.5 2 1049.0 3 1485.5 4 1089.0 5 849.0 6 1201.5 7 2160.5 8 3368.0 9 3978.0 10 3576.5 11 2969.5 12 3102.0 13 3581.5 14 4090.5 15 4738.5 16 5443.0 17 6119.5 18 6786.0 19 7418.0 20 8158.0 21 9125.0 22 10370.0 23 12240.5 24 14675.5 25 17683.0 26 21728.5 27 26703.5 28 32613.5 29 41216.0 30 52917.5 31 64454.5 32 78889.5 33 94637.0 34 107677.5 35 120906.0 36 134495.0 37 151399.5 38 161174.0 39 161113.0 40 159146.5 41 156885.0 42 159587.5 43 167052.0 44 177240.5 45 194741.0 46 210574.5 47 221050.0 48 231109.0 49 233636.5 50 228993.5 51 227894.0 52 224228.5 53 213280.0 54 202033.5 55 181557.0 56 159391.0 57 136253.5 58 114052.0 59 102016.0 60 84497.5 61 63672.5 62 49129.5 63 38704.0 64 28058.5 65 17572.0 66 11812.0 67 8750.5 68 6565.5 69 4778.5 70 3523.5 71 2598.0 72 1979.5 73 1517.5 74 1141.0 75 753.5 76 455.5 77 244.0 78 125.0 79 73.0 80 55.5 81 38.0 82 26.0 83 21.0 84 11.5 85 8.0 86 5.5 87 5.0 88 3.0 89 1.0 90 0.5 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0439636763977863 2 0.0 3 0.0 4 0.035780521505257165 5 0.0 6 0.0 7 9.236066470123172E-4 8 0.0 9 0.012191607740562587 10-11 4.710393899762818E-4 12-13 1.8472132940246344E-5 14-15 9.236066470123172E-5 16-17 2.4937379469332563E-4 18-19 0.0 20-21 2.7708199410369517E-5 22-23 0.0 24-25 5.818721876177599E-4 26-27 5.449279217372672E-4 28-29 1.7548526293234028E-4 30-31 0.002521446146343626 32-33 0.015544299869217298 34-35 0.026479802569843135 36-37 0.03169818012546272 38-39 0.022489821854749924 40-41 0.0013022853722873672 42-43 6.46524652908622E-5 44-45 0.01225626020585345 46-47 0.01825046734496339 48-49 0.0033988724610053275 50-51 0.011554319154124087 52-53 0.011609735552944827 54-55 0.010252033781836721 56-57 4.3409512409578905E-4 58-59 0.0090882894066012 60-61 0.004895115229165282 62-63 0.001394646036988599 64-65 0.03712898720989515 66-67 0.06163227155513192 68-69 0.05994207139109938 70-71 0.053827795387877846 72-73 0.04654053894295067 74-75 0.03641781009169567 76-77 0.03228928837955061 78-79 0.015590480201567915 80-81 0.0011637443752355197 82-83 0.039299462830374096 84-85 0.05307043793732775 86-87 0.0588706876805651 88-89 0.07091451835760572 90-91 0.04968080154279254 92-93 0.04024154161032666 94-95 0.01377097510695365 96-97 0.0037683151198102545 98-99 0.022443641522399308 100 0.032769563835997016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5413560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.36545531125185 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35037589950103 53.454742015888726 2 13.151240927487207 17.718786659762912 3 3.7520218293317233 7.582699766120626 4 1.4813391604217792 3.9916434804880274 5 0.7120462179975382 2.3983658839029522 6 0.4013989966305886 1.6224255701699546 7 0.2592367530530616 1.2224521331981033 8 0.17336319238916106 0.9342952311606388 9 0.12945579534313162 0.7848763736373268 >10 0.5507172984667562 6.320155245877486 >50 0.026283247311828885 1.1977075167921387 >100 0.011670689473658482 1.4115481002346029 >500 5.557645693663014E-4 0.2574944698118518 >1k 1.9615201544707616E-4 0.2707742159484887 >5k 3.2692002574220125E-5 0.18572094047040044 >10k+ 6.538400514844025E-5 0.646312396535615 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15865 0.2930603890970082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13482 0.2490412963004012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8433 0.15577549708509741 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.388853176098538E-5 2 0.0 0.0 0.0 0.0 7.388853176098538E-5 3 0.0 0.0 0.0 0.0 7.388853176098538E-5 4 0.0 1.8472132940246344E-5 0.0 0.0 1.1083279764147807E-4 5 0.0 1.8472132940246344E-5 0.0 0.0 1.1083279764147807E-4 6 0.0 1.8472132940246344E-5 0.0 0.0 1.1083279764147807E-4 7 0.0 1.8472132940246344E-5 0.0 0.0 1.293049305817244E-4 8 0.0 1.8472132940246344E-5 0.0 0.0 1.293049305817244E-4 9 0.0 1.8472132940246344E-5 0.0 0.0 1.4777706352197076E-4 10-11 1.8472132940246344E-5 1.8472132940246344E-5 0.0 0.0 1.4777706352197076E-4 12-13 1.8472132940246344E-5 1.8472132940246344E-5 0.0 9.236066470123172E-6 1.4777706352197076E-4 14-15 1.8472132940246344E-5 2.7708199410369517E-5 0.0 1.8472132940246344E-5 1.662491964622171E-4 16-17 2.7708199410369517E-5 3.694426588049269E-5 0.0 1.8472132940246344E-5 1.8472132940246344E-4 18-19 3.694426588049269E-5 3.694426588049269E-5 0.0 1.8472132940246344E-5 2.2166559528295613E-4 20-21 3.694426588049269E-5 3.694426588049269E-5 0.0 6.465246529086221E-5 2.309016617530793E-4 22-23 3.694426588049269E-5 3.694426588049269E-5 0.0 1.662491964622171E-4 3.23262326454311E-4 24-25 4.618033235061586E-5 3.694426588049269E-5 0.0 5.172197223268977E-4 4.0638692468541955E-4 26-27 5.5416398820739033E-5 3.694426588049269E-5 0.0 0.0013299935716977367 4.0638692468541955E-4 28-29 5.5416398820739033E-5 3.694426588049269E-5 0.0 0.0044887283044798615 4.0638692468541955E-4 30-31 5.5416398820739033E-5 3.694426588049269E-5 0.0 0.016791168842683925 4.340951240957891E-4 32-33 5.5416398820739033E-5 3.694426588049269E-5 0.0 0.04562616836240847 4.618033235061586E-4 34-35 5.5416398820739033E-5 3.694426588049269E-5 0.0 0.08664353955622547 5.172197223268976E-4 36-37 5.5416398820739033E-5 4.618033235061586E-5 0.0 0.1542053657851765 5.35691855267144E-4 38-39 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.2534653721395902 5.449279217372672E-4 40-41 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.3620815138282387 5.726361211476367E-4 42-43 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.4663197600100488 5.818721876177599E-4 44-45 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.5864070962545904 5.91108254087883E-4 46-47 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.7317920185607991 6.003443205580062E-4 48-49 5.5416398820739033E-5 5.5416398820739033E-5 0.0 0.8900889617922403 6.280525199683757E-4 50-51 5.5416398820739033E-5 5.5416398820739033E-5 0.0 1.0513229741611805 6.280525199683757E-4 52-53 5.5416398820739033E-5 5.5416398820739033E-5 0.0 1.209278182933227 7.01941051729361E-4 54-55 5.5416398820739033E-5 5.5416398820739033E-5 0.0 1.3818725570604187 7.943017164305928E-4 56-57 5.5416398820739033E-5 5.5416398820739033E-5 0.0 1.5757930086671248 8.127738493708391E-4 58-59 5.5416398820739033E-5 5.5416398820739033E-5 0.0 1.8005803944169825 8.220099158409624E-4 60-61 5.5416398820739033E-5 5.5416398820739033E-5 0.0 2.0245457702509997 8.497181152513319E-4 62-63 5.5416398820739033E-5 5.5416398820739033E-5 0.0 2.253498621978883 8.866623811318245E-4 64-65 5.5416398820739033E-5 5.5416398820739033E-5 0.0 2.4873650610688713 8.866623811318245E-4 66-67 5.5416398820739033E-5 5.5416398820739033E-5 0.0 2.747517345332831 8.866623811318245E-4 68-69 5.5416398820739033E-5 5.5416398820739033E-5 0.0 3.0375944849599894 8.866623811318245E-4 70-71 5.5416398820739033E-5 5.5416398820739033E-5 0.0 3.34131329476352 9.328427134824404E-4 72-73 5.5416398820739033E-5 5.5416398820739033E-5 0.0 3.6435635699983004 0.0011083279764147807 74-75 5.5416398820739033E-5 5.5416398820739033E-5 0.0 3.943218510554977 0.0011083279764147807 76-77 5.5416398820739033E-5 5.5416398820739033E-5 0.0 4.277379395444033 0.0011083279764147807 78-79 5.5416398820739033E-5 5.5416398820739033E-5 0.0 4.637770709108239 0.0011083279764147807 80-81 5.5416398820739033E-5 5.5416398820739033E-5 0.0 5.021224480748343 0.0011360361758251502 82-83 5.5416398820739033E-5 5.5416398820739033E-5 0.0 5.400078691286325 0.0012006886411160124 84-85 5.5416398820739033E-5 5.5416398820739033E-5 0.0 5.7831907284670345 0.0012006886411160124 86-87 5.5416398820739033E-5 5.5416398820739033E-5 0.0 6.186714472546717 0.0012006886411160124 88 5.5416398820739033E-5 5.5416398820739033E-5 0.0 6.508545208698158 0.0012191607740562587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8840 0.0 47.759506 1 GTATCAA 15310 0.0 40.07477 1 TACCTGG 4260 0.0 38.282036 2 CAACGCA 18070 0.0 32.81619 5 ATCAACG 18150 0.0 32.709217 3 GTACCTG 5160 0.0 31.981081 1 ACCTGGG 5215 0.0 31.186398 3 TATCAAC 19730 0.0 30.75207 2 AACGCAG 19990 0.0 30.463451 6 TCAACGC 20060 0.0 29.618263 4 GTACATG 19325 0.0 27.466934 1 ACGCAGA 21975 0.0 26.963305 7 TACATGG 19535 0.0 26.487993 2 CGCAGAG 22425 0.0 26.359133 8 GTACTAG 3130 0.0 26.136091 1 ACATGGG 19435 0.0 25.636854 3 GTGGTAT 3820 0.0 24.245914 1 CTGTGCG 4100 0.0 23.837591 9 GCAGAGT 24680 0.0 23.684168 9 CATGGGG 14825 0.0 23.39942 4 >>END_MODULE