FastQCFastQC Report
Mon 6 Jun 2016
ERR1211221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730567
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187970.2792781053958753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148610.22079863405267344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90920.135085201588514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103350.047.723851
GTATCAA186550.040.8447531
CAACGCA212400.034.614895
GTACTAG47000.034.045971
ATCAACG215400.033.936373
TATCAAC233700.032.118432
AACGCAG242400.031.2430446
GCATCGT46900.030.8697248
TCAACGC243050.030.3851434
TACCTGG45450.028.8883462
ACGCAGA261100.028.1591977
GTACATG257450.028.133931
GTGGTAT35350.027.6922721
TAGGCAT60300.027.6752645
CGCAGAG266400.027.5614268
CTGTGCG61750.027.5562469
TACATGG260050.027.0181312
TAGTACT66950.026.2604264
ACATGGG259000.026.2088623
GGGCACC55300.026.00504594
Produced by FastQC (version 0.11.5)