FastQCFastQC Report
Mon 6 Jun 2016
ERR1211221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730567
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173110.25719972774953437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148420.22051633985665695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89900.13356972748358348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113950.046.70991
GTATCAA192900.038.2931251
GTACTAG46600.033.196081
CAACGCA221350.032.138315
ATCAACG224550.031.772043
TATCAAC244800.030.1584632
AACGCAG247000.029.4096056
TCAACGC254600.028.169724
TACCTGG46150.027.9012282
TAGGCAT61650.027.2851685
CTGTGCG64550.027.2229969
GTGGTAT41700.027.061511
GGGCACC56750.026.09420694
ACGCAGA272950.026.0627027
TAGTACT67800.025.9948884
GTACATG262250.025.9615081
CTAGGCA68550.025.7104824
CTAACGC19900.025.270693
CGCAGAG280300.025.261018
TACATGG264200.025.2580592
Produced by FastQC (version 0.11.5)