##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211221_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6730567 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13072316195649 33.0 31.0 34.0 30.0 34.0 2 32.27179463483537 34.0 31.0 34.0 30.0 34.0 3 32.352098864776174 34.0 31.0 34.0 30.0 34.0 4 35.97197041497395 37.0 35.0 37.0 35.0 37.0 5 35.85665353305301 37.0 35.0 37.0 35.0 37.0 6 35.88072045044645 37.0 35.0 37.0 35.0 37.0 7 35.81527841562234 37.0 35.0 37.0 35.0 37.0 8 35.800159778514946 37.0 35.0 37.0 33.0 37.0 9 37.40398691521829 39.0 37.0 39.0 34.0 39.0 10-11 37.37921671383704 39.0 37.0 39.0 34.0 39.0 12-13 37.27773254467269 39.0 37.0 39.0 34.0 39.0 14-15 38.50044936778729 40.0 38.0 41.0 34.0 41.0 16-17 38.45226085707192 40.0 38.0 41.0 34.0 41.0 18-19 38.46693228074247 40.0 38.0 41.0 34.0 41.0 20-21 38.42327667193567 40.0 38.0 41.0 34.0 41.0 22-23 38.29550913021147 40.0 38.0 41.0 34.0 41.0 24-25 38.23367912391333 40.0 38.0 41.0 34.0 41.0 26-27 38.09594503702289 40.0 37.0 41.0 33.0 41.0 28-29 38.07165845017218 40.0 37.5 41.0 33.0 41.0 30-31 38.092074932171386 40.0 37.5 41.0 33.0 41.0 32-33 37.96780219259388 40.0 37.0 41.0 33.0 41.0 34-35 37.830779635653286 40.0 37.0 41.0 33.0 41.0 36-37 37.69231968123934 40.0 37.0 41.0 33.0 41.0 38-39 37.60508148867696 40.0 37.0 41.0 33.0 41.0 40-41 37.504735336562284 40.0 36.0 41.0 32.0 41.0 42-43 37.36351335630415 40.0 36.0 41.0 32.0 41.0 44-45 37.22401649073548 39.5 36.0 41.0 31.0 41.0 46-47 36.98852756387389 39.0 36.0 41.0 31.0 41.0 48-49 36.753385487433675 39.0 35.0 41.0 30.5 41.0 50-51 36.551378360842406 39.0 35.0 40.5 30.0 41.0 52-53 36.3440213877969 39.0 35.0 40.0 30.0 41.0 54-55 36.10185033445177 38.0 35.0 40.0 29.0 41.0 56-57 35.840414187987435 38.0 35.0 40.0 29.0 41.0 58-59 35.55567420991426 38.0 34.0 40.0 28.0 41.0 60-61 35.28708814576841 37.0 34.0 40.0 28.0 41.0 62-63 34.977538965142166 37.0 34.0 40.0 27.5 41.0 64-65 34.60121242385671 36.0 33.5 39.0 27.0 41.0 66-67 34.293304041100846 35.5 33.0 39.0 26.0 40.0 68-69 33.96254379460156 35.0 33.0 38.5 26.0 40.0 70-71 33.64357386235068 35.0 33.0 38.0 26.0 40.0 72-73 33.310906867133184 35.0 32.0 37.0 26.0 39.5 74-75 32.977167302546725 35.0 32.0 37.0 25.5 39.0 76-77 31.99891755033417 34.0 30.5 35.5 24.5 38.0 78-79 32.4991642606039 35.0 32.0 36.0 25.0 38.0 80-81 32.433843909435865 35.0 32.0 35.5 25.0 37.0 82-83 32.18976395302208 34.5 32.0 35.0 25.0 37.0 84-85 31.946522261794584 34.0 31.5 35.0 25.0 36.5 86-87 31.715426724078373 34.0 31.0 35.0 24.0 36.0 88-89 31.506064496497842 34.0 31.0 35.0 24.0 36.0 90-91 31.296699371687406 34.0 31.0 35.0 23.5 35.0 92-93 31.116012365674393 34.0 31.0 35.0 23.0 35.0 94-95 30.9297975935757 34.0 30.5 35.0 22.5 35.0 96-97 30.761981122838534 34.0 30.0 35.0 20.0 35.0 98-99 30.590411476477392 34.0 30.0 35.0 20.0 35.0 100 28.784896874215796 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31861857126335735 1101 2 -0.33371990615188807 1101 3 -0.39418849815019286 1101 4 -0.22959727642231798 1101 5 -0.2734152579478675 1101 6 -0.3656022516970694 1101 7 -0.3440695469065176 1101 8 -0.34097920640132884 1101 9 -0.5552704331471006 1101 10-11 -0.5622328109668899 1101 12-13 -0.5389727779474001 1101 14-15 -0.7060791620878817 1101 16-17 -0.7364581591861352 1101 18-19 -0.7465897004447015 1101 20-21 -0.7495858914830862 1101 22-23 -0.7479867980732706 1101 24-25 -0.7417355617610326 1101 26-27 -0.9324198493321845 1101 28-29 -0.971616193434059 1101 30-31 -0.7571019243101418 1101 32-33 -0.8109780944233478 1101 34-35 -0.7893664505143363 1101 36-37 -0.7819183289841618 1101 38-39 -0.9000071381609018 1101 40-41 -0.8754599211480567 1101 42-43 -0.8764775535459179 1101 44-45 -0.9480051623971448 1101 46-47 -0.89574239264455 1101 48-49 -0.8692498884677704 1101 50-51 -0.9451063893929188 1101 52-53 -0.9516584128757657 1101 54-55 -0.7712094512803134 1101 56-57 -0.8700476861139421 1101 58-59 -0.89493310449879 1101 60-61 -0.9917709416822973 1101 62-63 -1.131864920024718 1101 64-65 -1.049377377322287 1101 66-67 -1.0993828822046865 1101 68-69 -1.025026863689348 1101 70-71 -1.1724474426948106 1101 72-73 -1.160030719351937 1101 74-75 -1.2314108978179306 1101 76-77 -1.2071113984227821 1101 78-79 -1.0464172065262076 1101 80-81 -1.0202703675884948 1101 82-83 -0.9199572269515102 1101 84-85 -0.9020826216881659 1101 86-87 -1.1049064885261934 1101 88-89 -1.1091103079393427 1101 90-91 -1.1214832533035768 1101 92-93 -1.103691076704191 1101 94-95 -1.0539754810702533 1101 96-97 -1.1807362194971383 1101 98-99 -1.3284860853510345 1101 100 -1.180052825456638 1102 1 -0.39558755927524203 1102 2 -0.34600793682233544 1102 3 -0.3990143759124116 1102 4 -0.27583828960582935 1102 5 -0.36212602334286004 1102 6 -0.3934541250533954 1102 7 -0.3706673217622125 1102 8 -0.3942556588074879 1102 9 -0.3721412065683225 1102 10-11 -0.5409380583284786 1102 12-13 -0.5476131964213877 1102 14-15 -0.6837956461720367 1102 16-17 -0.6997359137200974 1102 18-19 -0.5837329494456895 1102 20-21 -0.7124218297835725 1102 22-23 -0.7077223914166169 1102 24-25 -0.7806022089313629 1102 26-27 -0.810062261555963 1102 28-29 -0.7516016885841665 1102 30-31 -0.7132629099995427 1102 32-33 -0.7580081757102732 1102 34-35 -0.7365220483831152 1102 36-37 -0.8487838725478056 1102 38-39 -0.8151907488545689 1102 40-41 -0.8310886354142468 1102 42-43 -0.7608060184866616 1102 44-45 -0.8682928472566047 1102 46-47 -0.9292126806496057 1102 48-49 -0.8735561425235758 1102 50-51 -0.9289629931020613 1102 52-53 -0.974466141964875 1102 54-55 -0.9705014993352279 1102 56-57 -0.983982961823493 1102 58-59 -1.0526036512216947 1102 60-61 -1.078162371973626 1102 62-63 -1.022334571908317 1102 64-65 -0.9777379877427279 1102 66-67 -1.0832847935575387 1102 68-69 -1.0525670268609062 1102 70-71 -0.9749782134690861 1102 72-73 -0.9954683943920699 1102 74-75 -1.0662873771205028 1102 76-77 -0.9897994491588022 1102 78-79 -0.9302933421524244 1102 80-81 -0.8903447489710565 1102 82-83 -0.8812096924557977 1102 84-85 -1.0079888386013103 1102 86-87 -0.9702810957532186 1102 88-89 -1.021581703820349 1102 90-91 -1.0118642644378362 1102 92-93 -1.0170473324677083 1102 94-95 -1.042990432059014 1102 96-97 -1.2373789924743583 1102 98-99 -1.1976561816657991 1102 100 -1.254169192989682 1103 1 -0.40686204580336494 1103 2 -0.41344123691782997 1103 3 -0.449303228573811 1103 4 -0.23903218228453937 1103 5 -0.28596446633468986 1103 6 -0.38382433128628435 1103 7 -0.40552600818165274 1103 8 -0.38853964713731415 1103 9 -0.48212564281762127 1103 10-11 -0.5055036977876028 1103 12-13 -0.5638046938652437 1103 14-15 -0.6947843664096567 1103 16-17 -0.6915183324852379 1103 18-19 -0.7292117853826525 1103 20-21 -0.7481252849362292 1103 22-23 -0.740616906117765 1103 24-25 -0.8253634768230782 1103 26-27 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-0.8297151593828289 1104 48-49 -0.8839025650145729 1104 50-51 -0.7982274999925494 1104 52-53 -0.7849322863349286 1104 54-55 -0.843983915075853 1104 56-57 -0.8189596396798251 1104 58-59 -0.963854690990523 1104 60-61 -0.8349816736567917 1104 62-63 -0.9144754908821326 1104 64-65 -0.9151836606752326 1104 66-67 -0.906589967733133 1104 68-69 -0.9038219860616081 1104 70-71 -1.0806148904383193 1104 72-73 -1.0179235647733194 1104 74-75 -1.0637122347060703 1104 76-77 -0.9293258684839891 1104 78-79 -0.7596288602353134 1104 80-81 -0.7688011066385023 1104 82-83 -0.7450340825684449 1104 84-85 -0.7467514259266572 1104 86-87 -0.8621833435037729 1104 88-89 -0.8779094315988303 1104 90-91 -0.8630087826882615 1104 92-93 -0.8983469073948633 1104 94-95 -0.950667998929525 1104 96-97 -1.0238662597337829 1104 98-99 -1.0345500334866777 1104 100 -1.116514888351972 1105 1 -0.4444529639398134 1105 2 -0.3955115148746202 1105 3 -0.47999089191016253 1105 4 -0.30523397540961383 1105 5 -0.3388294468891786 1105 6 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1105 68-69 -0.9200616060556754 1105 70-71 -1.1330833231795765 1105 72-73 -1.0596715247157746 1105 74-75 -0.9713044844906804 1105 76-77 -1.0134772018669977 1105 78-79 -0.9152842230604925 1105 80-81 -0.8907977844904948 1105 82-83 -0.841920649219901 1105 84-85 -0.8835456722977533 1105 86-87 -0.9555789136825368 1105 88-89 -0.946202176449976 1105 90-91 -1.0550613997659362 1105 92-93 -1.0165726668493136 1105 94-95 -0.9142824808398977 1105 96-97 -1.0331540279792755 1105 98-99 -1.0932618423192402 1105 100 -1.1625921402393793 1106 1 -0.42013521863858827 1106 2 -0.410456561016737 1106 3 -0.45321497564729896 1106 4 -0.2830129246146029 1106 5 -0.3996638110901003 1106 6 -0.3785592885323652 1106 7 -0.4335759981899656 1106 8 -0.40967643864753 1106 9 -0.5408705812372503 1106 10-11 -0.6533413380656867 1106 12-13 -0.7007439233480923 1106 14-15 -0.7518559332120063 1106 16-17 -0.6552058186809262 1106 18-19 -0.7255748361088905 1106 20-21 -0.8159548529796439 1106 22-23 -0.7168239286989859 1106 24-25 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2311 28-29 0.6881875526482943 2311 30-31 0.9547010044697117 2311 32-33 1.0029538735870318 2311 34-35 1.0553960589420583 2311 36-37 1.0972242463967987 2311 38-39 1.1572916770882102 2311 40-41 1.1139215632353867 2311 42-43 1.1045971654641278 2311 44-45 1.126811451133598 2311 46-47 0.709771528516157 2311 48-49 1.0113680530106706 2311 50-51 1.1720765512749693 2311 52-53 1.159419075506385 2311 54-55 1.1366038930404443 2311 56-57 1.1636979571909336 2311 58-59 1.1759504940638408 2311 60-61 1.2285668879405307 2311 62-63 1.3242383370270474 2311 64-65 1.3149796217590861 2311 66-67 1.341083216628114 2311 68-69 1.33738245626094 2311 70-71 1.3402043661779857 2311 72-73 1.3501293300914838 2311 74-75 1.3410721819940221 2311 76-77 1.3134783765150786 2311 78-79 1.0011379782787202 2311 80-81 0.9356076246842804 2311 82-83 1.0533269443983215 2311 84-85 1.102076502379699 2311 86-87 1.1453876728268817 2311 88-89 1.19942813905082 2311 90-91 1.2761739254492603 2311 92-93 1.3118959288615706 2311 94-95 1.355224237939801 2311 96-97 1.3669530434446138 2311 98-99 1.3848548581267544 2311 100 1.556391098135709 2312 1 0.3063477768319416 2312 2 0.39412452109269225 2312 3 0.43653358759485883 2312 4 0.19003566542718886 2312 5 0.3672513862841953 2312 6 0.4442543911889203 2312 7 0.3288650674426563 2312 8 0.41896913792496093 2312 9 0.6199075012989894 2312 10-11 0.6595944453933242 2312 12-13 0.7343532501182892 2312 14-15 0.9541886650086369 2312 16-17 0.8577802140807975 2312 18-19 0.8770421393049332 2312 20-21 0.8839269887834433 2312 22-23 0.9835170634827932 2312 24-25 0.9787022915839643 2312 26-27 1.0052448346764251 2312 28-29 1.0148391252494733 2312 30-31 0.9787390367882551 2312 32-33 0.8534072535373483 2312 34-35 0.9709234606446344 2312 36-37 1.0178974268376209 2312 38-39 0.8703562603449555 2312 40-41 1.0607415359794174 2312 42-43 1.115044269714275 2312 44-45 0.8530502454639404 2312 46-47 0.9077820133993271 2312 48-49 1.0937416396652182 2312 50-51 0.9532429846816513 2312 52-53 1.0966135618179962 2312 54-55 0.9523245876212911 2312 56-57 0.9799966923353765 2312 58-59 1.0918355975472522 2312 60-61 1.1382631729360995 2312 62-63 1.1699899308973514 2312 64-65 1.1201577636791953 2312 66-67 1.1655814888412124 2312 68-69 1.2167004465139044 2312 70-71 1.0631215689487448 2312 72-73 1.0799037856285452 2312 74-75 1.1686183372742818 2312 76-77 1.1401314589604468 2312 78-79 1.0361035735249402 2312 80-81 0.9464161396803163 2312 82-83 0.9363867880572343 2312 84-85 1.0031355267840318 2312 86-87 1.089859378346869 2312 88-89 1.0741344087116254 2312 90-91 1.0941316511250143 2312 92-93 1.1384800179922685 2312 94-95 1.175421981576136 2312 96-97 0.8674793882562604 2312 98-99 0.5737088601328786 2312 100 0.779250664245879 2313 1 0.06643095889449313 2313 2 0.2272878655861632 2313 3 0.3143489032358602 2313 4 0.2170913655321698 2313 5 0.37644066484063643 2313 6 0.2548801879111906 2313 7 0.2788557164497689 2313 8 0.42849233690284194 2313 9 0.6180378168644722 2313 10-11 0.41241425979378477 2313 12-13 0.46154972926058946 2313 14-15 0.5120314487895783 2313 16-17 0.8044199058643571 2313 18-19 0.7537487838762615 2313 20-21 0.7267473703489316 2313 22-23 0.7011881987029795 2313 24-25 0.6112751497968887 2313 26-27 0.8166036721198608 2313 28-29 0.680984811469358 2313 30-31 0.8419769899940874 2313 32-33 0.9172852998803549 2313 34-35 0.9560559362573429 2313 36-37 0.986116575228877 2313 38-39 0.9921532078723914 2313 40-41 1.030723185860893 2313 42-43 1.0657812307829744 2313 44-45 0.8870056206620873 2313 46-47 0.8595090791252389 2313 48-49 0.940145724988696 2313 50-51 0.9312754458019299 2313 52-53 0.9045605481151 2313 54-55 0.870020168779071 2313 56-57 0.6513407433204605 2313 58-59 0.7803560728476171 2313 60-61 0.5956188615692426 2313 62-63 0.9401284187807377 2313 64-65 0.9893457600891864 2313 66-67 0.9273367553621839 2313 68-69 1.0474974033602038 2313 70-71 0.9989756214794738 2313 72-73 1.0219570564034157 2313 74-75 1.05921303792843 2313 76-77 0.9780442161248004 2313 78-79 0.8874929109508614 2313 80-81 0.7803017359057236 2313 82-83 0.9047941613311963 2313 84-85 0.8792044722009074 2313 86-87 0.8776264640729856 2313 88-89 0.8577188692252058 2313 90-91 0.7519323313374464 2313 92-93 1.0551637719114133 2313 94-95 1.080111343841633 2313 96-97 1.116573305965595 2313 98-99 1.1049089540284136 2313 100 1.286733306264484 2314 1 -0.37802580541418607 2314 2 0.05303628215263956 2314 3 0.29672198102748126 2314 4 0.21195645857992673 2314 5 0.33911736358364664 2314 6 0.4115644704262067 2314 7 0.43027561662522373 2314 8 0.43783755356291465 2314 9 0.4738924616549838 2314 10-11 0.5965952176976543 2314 12-13 0.447086379976696 2314 14-15 0.7641044216972617 2314 16-17 0.8174943267376733 2314 18-19 0.8618857302410703 2314 20-21 0.8840833518768036 2314 22-23 0.9354518110588046 2314 24-25 0.8300581596543495 2314 26-27 0.7868820152329974 2314 28-29 0.8513288641592496 2314 30-31 0.9133101019436651 2314 32-33 0.9254046588991542 2314 34-35 0.9032049335520043 2314 36-37 0.8546538738826186 2314 38-39 0.6725406911208438 2314 40-41 0.7331699622952144 2314 42-43 0.9156660602999693 2314 44-45 0.9861990231084548 2314 46-47 0.912258945246009 2314 48-49 0.9227946680588204 2314 50-51 0.8824261016524702 2314 52-53 0.9533557611796724 2314 54-55 0.8954465930001518 2314 56-57 0.7998232202916213 2314 58-59 0.8845025980548229 2314 60-61 1.020724358778324 2314 62-63 0.9588976307017631 2314 64-65 1.010100370307761 2314 66-67 0.7026091618115444 2314 68-69 0.71660529551351 2314 70-71 0.9088856096855835 2314 72-73 1.0127870123261786 2314 74-75 1.0861070990732387 2314 76-77 0.8502771236097999 2314 78-79 0.8012960852799154 2314 80-81 0.8654018989718253 2314 82-83 0.8150820391782077 2314 84-85 0.8781613098914356 2314 86-87 0.9587715769191227 2314 88-89 0.9813940535594234 2314 90-91 1.0541232829095897 2314 92-93 0.8822557000438316 2314 94-95 0.9577351186439813 2314 96-97 0.9321344018777928 2314 98-99 1.0449251237334103 2314 100 1.130425067746991 2315 1 0.4022554529662443 2315 2 0.4400207984792459 2315 3 0.4265949043441495 2315 4 0.3140557751515658 2315 5 0.38202837285889757 2315 6 0.4362248641754647 2315 7 0.3941639842305378 2315 8 0.2835028566190019 2315 9 0.5070628626046059 2315 10-11 0.5710280121355922 2315 12-13 0.42410774663260753 2315 14-15 0.6242999344444442 2315 16-17 0.7903416829710395 2315 18-19 0.7686003496983815 2315 20-21 0.5554811694121327 2315 22-23 0.8695013414091761 2315 24-25 0.9332181743121382 2315 26-27 0.898647923721299 2315 28-29 0.8342325179487844 2315 30-31 0.8751344311265967 2315 32-33 0.7986875886321556 2315 34-35 0.7821619401052899 2315 36-37 0.8063864179806544 2315 38-39 0.8592909377950804 2315 40-41 0.840726288774789 2315 42-43 0.9201458793814226 2315 44-45 0.9062083528745077 2315 46-47 0.9333765771268432 2315 48-49 0.8518162177460127 2315 50-51 0.9391154114104125 2315 52-53 0.9741641380459853 2315 54-55 0.7912947462357351 2315 56-57 0.7855056377509868 2315 58-59 0.8319056407237468 2315 60-61 0.9409929522985934 2315 62-63 0.9873259915216579 2315 64-65 0.998728275575985 2315 66-67 1.0156583082027524 2315 68-69 1.0018727962104634 2315 70-71 0.9341015238645696 2315 72-73 0.9693556950528048 2315 74-75 0.4085272608068067 2315 76-77 0.4550530740447556 2315 78-79 0.7606861870134338 2315 80-81 0.7483452003698972 2315 82-83 0.7321027902278843 2315 84-85 0.7878617723770738 2315 86-87 0.8077497523487658 2315 88-89 0.7754900398664368 2315 90-91 0.8705386475912498 2315 92-93 0.9475742716044699 2315 94-95 0.9988844670833288 2315 96-97 1.0486111498972512 2315 98-99 1.085204103087019 2315 100 1.0177311496843338 2316 1 0.3167652757218704 2316 2 0.11524535274831749 2316 3 0.2280655998748955 2316 4 0.037967428159603855 2316 5 0.30614333439484653 2316 6 0.3792041872378533 2316 7 0.42971230387788495 2316 8 0.42083772118521523 2316 9 0.6247510804611238 2316 10-11 0.5968894399583391 2316 12-13 0.36107107829011653 2316 14-15 0.5038864386555773 2316 16-17 0.8070839714593987 2316 18-19 0.7694899817843606 2316 20-21 0.7314990719171703 2316 22-23 0.7867941663750315 2316 24-25 0.4572477616103967 2316 26-27 0.7297935243127185 2316 28-29 0.8437410122941671 2316 30-31 0.5113260880043953 2316 32-33 0.6478735988265782 2316 34-35 0.775691642368777 2316 36-37 0.8169945275972452 2316 38-39 0.7267427615377926 2316 40-41 0.7552991141580065 2316 42-43 0.7091696355609542 2316 44-45 0.6187264271944386 2316 46-47 0.8453609320369395 2316 48-49 0.9062041796716258 2316 50-51 0.8805654247098573 2316 52-53 0.8511414266421369 2316 54-55 0.7627058002508136 2316 56-57 0.8417086001173502 2316 58-59 0.748160587162694 2316 60-61 0.8919367987133455 2316 62-63 0.9337388427074131 2316 64-65 0.9573981299157808 2316 66-67 0.9209818154175551 2316 68-69 0.9161930356818218 2316 70-71 0.8808863989683147 2316 72-73 0.8683074209388977 2316 74-75 0.9563995756861488 2316 76-77 0.7859483136101062 2316 78-79 0.4831662564482784 2316 80-81 0.5107498093512106 2316 82-83 0.48757575989878177 2316 84-85 0.6023606241442074 2316 86-87 0.6790409003645088 2316 88-89 0.6665578216253927 2316 90-91 0.5554180706737597 2316 92-93 0.40605621516034773 2316 94-95 0.39938133778148455 2316 96-97 0.621194273739686 2316 98-99 0.8473902206130042 2316 100 0.4702506371903361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 30.0 13 158.0 14 550.0 15 1594.0 16 3790.0 17 7504.0 18 12896.0 19 19037.0 20 27526.0 21 38850.0 22 48717.0 23 51590.0 24 53234.0 25 58255.0 26 69121.0 27 84346.0 28 104864.0 29 129266.0 30 159540.0 31 200098.0 32 254718.0 33 334204.0 34 475093.0 35 658385.0 36 1024269.0 37 1503168.0 38 1221868.0 39 187873.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24978480563538 20.25037312495513 13.735085964035823 23.764756105373667 2 15.540577784902817 19.698444425261645 40.86866678542833 23.89231100440721 3 19.162664898811645 25.144642345882602 29.326786287098844 26.365906468206912 4 12.701813574499232 16.239625172310014 38.127540992134115 32.93102026105664 5 13.369185686733376 37.80329354124251 34.455269518897886 14.372251253126223 6 31.53266284995009 36.983644914314056 17.310785257765062 14.1729069779708 7 27.970103953304065 31.910511547247005 21.892848727037816 18.226535772411115 8 26.39360101459506 36.12873922806206 19.131761113142474 18.345898644200407 9 26.26408261354169 15.744309560953967 20.686580856813706 37.30502696869064 10-11 26.057122030958002 26.12768102639046 26.96880249394625 20.84639444870529 12-13 25.915236988630454 23.42991833301377 27.806452518737096 22.848392159618683 14-15 23.144882913885755 24.936918315402927 25.44410501416004 26.474093756551277 16-17 22.2887555597289 27.7794855809575 26.48133642689209 23.45042243242151 18-19 21.90141632941177 27.33165719916316 28.736546267201557 22.03038020422351 20-21 23.093671008698077 26.475956631885545 28.463612352421425 21.966760006994953 22-23 23.124188497046386 26.2731951111994 28.161364414023364 22.44125197773085 24-25 22.646914682282475 26.234633362110166 28.74319490863313 22.37525704697423 26-27 22.612886997410016 26.531668503073156 28.06585537912582 22.789589120391007 28-29 22.135742625030762 26.934018130901123 28.396082134998256 22.534157109069856 30-31 22.925612693077046 26.5219691700757 28.36931604008018 22.183102096767076 32-33 23.040730666834683 26.64629028739626 27.873442460417706 22.439536585351355 34-35 22.994572773777993 26.67545184220063 28.287918449158422 22.042056934862956 36-37 22.730122230303994 26.474575258536664 28.094585287598356 22.700717223560982 38-39 22.842120968851713 26.540836892286833 28.3827049275097 22.234337211351757 40-41 22.661139665730797 25.95095278214824 28.738282678031496 22.64962487408947 42-43 22.60107938956374 26.166182073759497 28.224508987991868 23.00822954868489 44-45 22.61075423416896 26.735722862662413 28.157006273409756 22.496516629758865 46-47 22.64023496824392 26.546596883535283 27.645272549491633 23.167895598729164 48-49 23.117096918220632 26.533947267347312 27.715673667029943 22.63328214740211 50-51 22.865611517632995 26.535059076309963 27.62881221066427 22.970517195392766 52-53 22.542668049558237 26.877156434160593 27.414099253179113 23.166076263102056 54-55 22.4891129632911 26.778287814505624 27.472703005204753 23.259896216998523 56-57 23.07950087823536 26.498980319861314 27.154172724871188 23.267346077032133 58-59 22.44834248093387 26.869725667080434 27.664020108178338 23.017911743807353 60-61 22.70098303824059 27.139008213207905 27.265132668782186 22.894876079769315 62-63 22.852181853543268 26.729483263707426 27.048271203907355 23.37006367884195 64-65 22.346256704858693 26.772936712316813 27.257952362405813 23.62285422041868 66-67 22.553593072486137 26.608780791514963 27.22846521012421 23.609160925874694 68-69 22.338557908913067 26.623169749622654 27.343949185674145 23.694323155790133 70-71 22.289913363416026 26.34225460555318 27.478773059540234 23.889058971490567 72-73 22.629889074585005 26.522360295671294 27.383618837707136 23.464131792036564 74-75 22.806140386534523 26.736101022052683 27.10680533423619 23.3509532571766 76-77 22.617725779697494 26.601963665514905 26.89100858299253 23.889301971795067 78-79 22.47286597600201 26.547444207159575 27.0602380867999 23.919451730038517 80-81 22.24703552217741 26.882600073947117 27.24336156730447 23.627002836571002 82-83 22.880916334152097 26.315014240432482 27.83393672973321 22.97013269568221 84-85 22.99190799288612 26.54739047784405 27.026197791272715 23.434503737997115 86-87 22.169225868035088 26.970383867569193 27.305916224614684 23.554474039781038 88-89 22.61031282549051 26.200483161637766 27.289934821425188 23.899269191446535 90-91 22.613066447085746 26.219302503559906 27.301554599311274 23.866076450043074 92-93 22.731826947729832 26.333805240022144 27.18428513568246 23.750082676565558 94-95 22.025139891017478 26.339490762073748 27.598104998095756 24.037264348813018 96-97 22.169182757979996 26.2213565534503 27.37154071649114 24.23791997207857 98-99 21.79873537239803 26.530377804839684 27.272737405235965 24.398149417526323 100 22.108983921406423 26.204378553136138 26.95405302935161 24.73258449610583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 818.0 1 583.0 2 582.0 3 855.5 4 700.5 5 569.0 6 909.0 7 1744.5 8 2778.5 9 3285.0 10 3051.0 11 2780.5 12 3007.5 13 3562.5 14 4159.0 15 4915.0 16 5786.0 17 6668.5 18 7482.0 19 8093.0 20 8870.0 21 9883.0 22 11203.5 23 13086.0 24 15316.0 25 18152.0 26 22316.0 27 27141.5 28 33375.5 29 43706.0 30 57441.5 31 71532.5 32 88823.0 33 107038.0 34 122621.5 35 138909.0 36 154969.0 37 178271.5 38 191945.0 39 190419.5 40 187095.0 41 183286.0 42 187354.0 43 198248.0 44 213297.5 45 239938.0 46 266008.5 47 284749.0 48 300323.0 49 302224.0 50 295310.0 51 298567.5 52 297230.5 53 283837.5 54 271595.0 55 242879.5 56 213495.5 57 183446.5 58 152866.5 59 137309.0 60 112286.0 61 82876.5 62 63492.5 63 50513.0 64 36681.5 65 22444.0 66 14876.0 67 10709.0 68 8037.0 69 6037.0 70 4540.5 71 3488.0 72 2594.5 73 1950.5 74 1461.5 75 969.5 76 631.5 77 386.5 78 207.5 79 126.0 80 88.5 81 55.5 82 32.0 83 23.5 84 14.5 85 8.5 86 10.0 87 9.0 88 3.5 89 2.0 90 2.5 91 1.5 92 0.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04350302136506479 2 0.0 3 0.0 4 0.03182495620354125 5 0.0 6 0.0 7 6.685915168811186E-4 8 0.0 9 0.010786609805682048 10-11 4.308700886567209E-4 12-13 2.228638389603729E-5 14-15 1.1886071411219887E-4 16-17 3.4172455307257175E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.423020081369073E-4 26-27 4.6058526718477063E-4 28-29 2.005774550643356E-4 30-31 0.0023474991037159276 32-33 0.015496465602377928 34-35 0.026855092594724933 36-37 0.031512946828996725 38-39 0.02271725398469401 40-41 0.0013891845961863244 42-43 5.2001562424087004E-5 44-45 0.010905470519794246 46-47 0.01704165488583651 48-49 0.0030012330313330215 50-51 0.010578603555985699 52-53 0.010504315609665575 54-55 0.009798580119624395 56-57 3.7143973160062144E-4 58-59 0.008453968291230143 60-61 0.004605852671847706 62-63 0.0010994616055378396 64-65 0.03462561177980993 66-67 0.05940064187757139 68-69 0.05883605348553844 70-71 0.051162108630669605 72-73 0.04499620908609928 74-75 0.03407588097704101 76-77 0.03013119102744241 78-79 0.014300429666623924 80-81 0.001210893525018026 82-83 0.037753134319887166 84-85 0.05103581912192539 86-87 0.05636969366771031 88-89 0.06924379476498786 90-91 0.047640859975095706 92-93 0.03776799190915119 94-95 0.012836957124117478 96-97 0.0037663988784303014 98-99 0.023036692153870542 100 0.03238954459557419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6730567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.6984384875756 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84966448162527 50.48902954736882 2 14.341805053874038 18.844683942654893 3 4.431590060567913 8.734456409891177 4 1.7852012109739568 4.691397277884719 5 0.869934647271226 2.8576673955979714 6 0.4908233474667843 1.9347796501087489 7 0.30135243984813875 1.3858869312710642 8 0.1960266403607871 1.0302915338935423 9 0.1370760112398071 0.8105111903304618 >10 0.5678191039784907 6.147041198498025 >50 0.019629382171213763 0.870889359589494 >100 0.008183816520619346 0.9421633484913006 >500 4.8753455461727314E-4 0.20705700587375667 >1k 3.2501563779395296E-4 0.3220391281786439 >5k 2.7084636458953676E-5 0.1599697071798867 >10k+ 5.416927291790735E-5 0.5721363731874191 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17311 0.25719972774953437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14842 0.22051633985665695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8990 0.13356972748358348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9715178528049717E-5 2 0.0 0.0 0.0 1.4857589264024859E-5 2.9715178528049717E-5 3 0.0 0.0 0.0 1.4857589264024859E-5 2.9715178528049717E-5 4 0.0 0.0 0.0 2.9715178528049717E-5 2.9715178528049717E-5 5 0.0 0.0 0.0 2.9715178528049717E-5 2.9715178528049717E-5 6 0.0 0.0 0.0 2.9715178528049717E-5 4.4572767792074574E-5 7 0.0 1.4857589264024859E-5 0.0 2.9715178528049717E-5 5.9430357056099434E-5 8 0.0 1.4857589264024859E-5 0.0 2.9715178528049717E-5 7.428794632012429E-5 9 0.0 1.4857589264024859E-5 0.0 2.9715178528049717E-5 8.914553558414915E-5 10-11 0.0 1.4857589264024859E-5 0.0 2.9715178528049717E-5 8.914553558414915E-5 12-13 0.0 1.4857589264024859E-5 0.0 2.9715178528049717E-5 8.914553558414915E-5 14-15 0.0 1.4857589264024859E-5 0.0 3.7143973160062144E-5 1.5600468727226103E-4 16-17 0.0 1.4857589264024859E-5 0.0 4.4572767792074574E-5 1.782910711682983E-4 18-19 7.428794632012429E-6 1.4857589264024859E-5 0.0 5.943035705609943E-5 1.9314866043232317E-4 20-21 2.2286383896037287E-5 1.4857589264024859E-5 0.0 7.428794632012429E-5 2.2286383896037286E-4 22-23 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.4857589264024858E-4 2.8972299064848476E-4 24-25 2.9715178528049717E-5 1.4857589264024859E-5 0.0 3.268669638085469E-4 3.565821423365966E-4 26-27 2.9715178528049717E-5 1.4857589264024859E-5 0.0 8.765977665774667E-4 3.7143973160062144E-4 28-29 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.0029938042367010087 3.788685262326339E-4 30-31 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.013356972748358347 3.8629732086464634E-4 32-33 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.039053173380489345 4.1601249939269603E-4 34-35 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.07728917935145732 4.903004457128203E-4 36-37 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.14121395715992427 4.977292403448327E-4 38-39 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.23474248157696076 5.125868296088576E-4 40-41 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.3339243187089587 5.645883920329446E-4 42-43 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.42922832504304614 5.794459812969695E-4 44-45 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.5428963117074683 6.091611598250192E-4 46-47 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.677216347448885 6.091611598250192E-4 48-49 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.8255619474555412 6.388763383530689E-4 50-51 2.9715178528049717E-5 1.4857589264024859E-5 0.0 0.9762476177712813 6.388763383530689E-4 52-53 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.1227805918877265 6.685915168811187E-4 54-55 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.2851740425435183 6.983066954091684E-4 56-57 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.469623584461755 6.983066954091684E-4 58-59 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.683721445756353 7.057354900411808E-4 60-61 2.9715178528049717E-5 1.4857589264024859E-5 0.0 1.898205604371816 7.131642846731932E-4 62-63 2.9715178528049717E-5 1.4857589264024859E-5 0.0 2.11479954066277 7.428794632012429E-4 64-65 2.9715178528049717E-5 1.4857589264024859E-5 0.0 2.3349815847609867 7.428794632012429E-4 66-67 2.9715178528049717E-5 1.4857589264024859E-5 0.0 2.5821152957841442 7.428794632012429E-4 68-69 2.9715178528049717E-5 1.4857589264024859E-5 0.0 2.858637318371543 7.503082578332553E-4 70-71 2.9715178528049717E-5 1.4857589264024859E-5 0.0 3.1548085027606145 7.577370524652678E-4 72-73 2.9715178528049717E-5 2.2286383896037287E-5 0.0 3.4439594762224344 8.765977665774667E-4 74-75 2.9715178528049717E-5 2.9715178528049717E-5 0.0 3.726573110408083 8.765977665774667E-4 76-77 2.9715178528049717E-5 2.9715178528049717E-5 0.0 4.0422300231169235 8.914553558414916E-4 78-79 2.9715178528049717E-5 2.9715178528049717E-5 0.0 4.387787834219614 9.063129451055164E-4 80-81 2.9715178528049717E-5 2.9715178528049717E-5 0.0 4.754265131006051 9.657433021616159E-4 82-83 2.9715178528049717E-5 2.9715178528049717E-5 0.0 5.124367679572909 0.0010251736592177152 84-85 2.9715178528049717E-5 2.9715178528049717E-5 0.0 5.488868916987231 0.0010251736592177152 86-87 2.9715178528049717E-5 2.9715178528049717E-5 0.0 5.871355266205655 0.0010251736592177152 88 2.9715178528049717E-5 2.9715178528049717E-5 0.0 6.181663446779447 0.00104003124848174 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11395 0.0 46.7099 1 GTATCAA 19290 0.0 38.293125 1 GTACTAG 4660 0.0 33.19608 1 CAACGCA 22135 0.0 32.13831 5 ATCAACG 22455 0.0 31.77204 3 TATCAAC 24480 0.0 30.158463 2 AACGCAG 24700 0.0 29.409605 6 TCAACGC 25460 0.0 28.16972 4 TACCTGG 4615 0.0 27.901228 2 TAGGCAT 6165 0.0 27.285168 5 CTGTGCG 6455 0.0 27.222996 9 GTGGTAT 4170 0.0 27.06151 1 GGGCACC 5675 0.0 26.094206 94 ACGCAGA 27295 0.0 26.062702 7 TAGTACT 6780 0.0 25.994888 4 GTACATG 26225 0.0 25.961508 1 CTAGGCA 6855 0.0 25.710482 4 CTAACGC 1990 0.0 25.27069 3 CGCAGAG 28030 0.0 25.26101 8 TACATGG 26420 0.0 25.258059 2 >>END_MODULE