FastQCFastQC Report
Mon 6 Jun 2016
ERR1211220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8292365
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199810.2409565907916499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162080.19545690523752873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95240.1148526385415982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130750.046.9358371
GTATCAA229200.038.7869831
GTACTAG75700.036.1822661
GCATCGT74800.032.9286548
CAACGCA251650.032.913715
ATCAACG257450.032.2815673
TATCAAC286550.030.4823782
TAGGCAT94150.030.0568895
AACGCAG290700.029.1395516
GTACATG337100.028.5637721
GGGCACC85250.028.33726994
CTAGGCA106700.027.7106194
TACCTGG48550.027.6265662
TAGTACT104950.027.545634
CTGTGCG103550.027.5091579
GTGGTAT45650.027.3195651
TACATGG344750.027.1929512
TCAACGC309550.027.0909374
ACGCAGA310250.026.6067037
ACATGGG341250.026.4342863
Produced by FastQC (version 0.11.5)