##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8292365 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.169459376185202 31.0 31.0 34.0 28.0 34.0 2 31.186601651036828 31.0 31.0 34.0 28.0 34.0 3 31.246371330736164 31.0 31.0 34.0 27.0 34.0 4 34.83001158294407 37.0 35.0 37.0 32.0 37.0 5 34.888266616339244 37.0 35.0 37.0 32.0 37.0 6 34.950511343868726 37.0 35.0 37.0 32.0 37.0 7 34.849388322872905 37.0 35.0 37.0 32.0 37.0 8 34.85212156001334 37.0 35.0 37.0 32.0 37.0 9 36.36200746108016 39.0 37.0 39.0 32.0 39.0 10-11 36.30793464831807 39.0 36.0 39.0 31.0 39.0 12-13 36.19202422951715 39.0 35.0 39.0 31.0 39.0 14-15 37.34466928312972 40.0 37.0 41.0 31.0 41.0 16-17 37.31781500211339 40.0 37.0 41.0 31.0 41.0 18-19 37.321003718480796 40.0 37.0 41.0 31.0 41.0 20-21 37.27994233249501 40.0 37.0 41.0 31.0 41.0 22-23 37.162796801636205 40.0 36.0 41.0 30.5 41.0 24-25 37.06266137585598 40.0 36.0 41.0 30.0 41.0 26-27 36.91173724263223 39.5 36.0 41.0 30.0 41.0 28-29 36.78547483136596 39.0 36.0 41.0 30.0 41.0 30-31 36.633834678044195 39.0 35.5 41.0 30.0 41.0 32-33 36.463000844752976 39.0 35.0 41.0 29.0 41.0 34-35 36.317663356593684 39.0 35.0 40.5 29.0 41.0 36-37 36.21376682044266 39.0 35.0 40.0 28.5 41.0 38-39 36.06587065330578 39.0 35.0 40.0 28.0 41.0 40-41 35.89707658792154 38.0 35.0 40.0 27.0 41.0 42-43 35.67936203965937 38.0 34.0 40.0 27.0 41.0 44-45 35.44825034836262 38.0 34.0 40.0 26.0 41.0 46-47 35.217441948105275 38.0 34.0 40.0 25.5 41.0 48-49 35.00495775330681 38.0 33.0 40.0 24.5 41.0 50-51 34.33319722419358 37.5 32.5 39.5 24.0 40.5 52-53 34.448894555413325 37.5 33.0 39.5 24.5 40.5 54-55 34.92149869186896 38.0 33.5 40.0 26.0 41.0 56-57 34.86604068923643 37.5 33.5 40.0 25.5 41.0 58-59 34.6349969520155 37.0 33.0 40.0 25.0 41.0 60-61 34.41025045327841 37.0 33.0 40.0 25.0 41.0 62-63 34.100444264091124 36.0 33.0 39.5 24.5 41.0 64-65 33.84107820868956 36.0 32.5 39.0 24.0 41.0 66-67 33.668488422784094 35.0 32.0 39.0 24.0 41.0 68-69 33.369407400663135 35.0 32.0 39.0 23.0 41.0 70-71 33.087944151035316 35.0 32.0 38.0 23.0 40.0 72-73 32.765757718093695 35.0 31.5 37.0 23.0 40.0 74-75 32.44414759842337 35.0 31.0 37.0 22.0 39.0 76-77 32.132248399582025 35.0 31.0 36.0 21.5 39.0 78-79 31.809202441040643 35.0 31.0 36.0 20.0 39.0 80-81 31.527168485709446 35.0 30.5 35.5 20.0 37.5 82-83 31.25232916061944 34.0 30.0 35.0 20.0 37.0 84-85 31.016872810109057 34.0 30.0 35.0 20.0 37.0 86-87 30.803095678976987 34.0 30.0 35.0 19.0 36.0 88-89 30.60409527318202 34.0 29.5 35.0 19.0 36.0 90-91 30.381374493283882 34.0 29.0 35.0 18.0 36.0 92-93 30.18985199035498 34.0 29.0 35.0 18.0 35.0 94-95 30.02350722622557 34.0 29.0 35.0 18.0 35.0 96-97 29.841286954927817 34.0 29.0 35.0 17.0 35.0 98-99 29.602642430717896 34.0 28.5 35.0 16.0 35.0 100 28.429079038368428 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.529556622080662 1101 2 -0.47966306702725703 1101 3 -0.49790648721667097 1101 4 -0.4987527479250744 1101 5 -0.43930063288460275 1101 6 -0.3888982030402559 1101 7 -0.4590304513697916 1101 8 -0.3763210209538741 1101 9 -0.6493821235502395 1101 10-11 -0.6469665851356297 1101 12-13 -0.6325218511940207 1101 14-15 -0.7796691943095126 1101 16-17 -0.8105903452777312 1101 18-19 -0.8038437354479413 1101 20-21 -0.772002542347856 1101 22-23 -0.8391565232548501 1101 24-25 -0.951221732654254 1101 26-27 -0.9968281577277409 1101 28-29 -0.94816849922519 1101 30-31 -1.0557568720814174 1101 32-33 -1.0119765284477964 1101 34-35 -0.9993702306344119 1101 36-37 -1.0694416792699784 1101 38-39 -1.2072767864744094 1101 40-41 -1.1839681181896324 1101 42-43 -1.2281545112252985 1101 44-45 -1.2761726043198465 1101 46-47 -1.3571941109915713 1101 48-49 -1.4302844634721694 1101 50-51 -1.434038936823974 1101 52-53 -1.2932667197428316 1101 54-55 -1.1352107344605216 1101 56-57 -1.038585101315249 1101 58-59 -1.0036893843679238 1101 60-61 -1.056832377000525 1101 62-63 -1.1228218091652025 1101 64-65 -1.0569815977119958 1101 66-67 -1.1416422991255288 1101 68-69 -1.1245072287358013 1101 70-71 -1.166733011304654 1101 72-73 -1.1896076364496828 1101 74-75 -1.2614782820361015 1101 76-77 -1.2413540702625738 1101 78-79 -1.2043835086129064 1101 80-81 -1.3310295301309019 1101 82-83 -1.305597373268693 1101 84-85 -1.5097536760156807 1101 86-87 -1.442754749606312 1101 88-89 -1.4925327430274287 1101 90-91 -1.6275446260833313 1101 92-93 -1.7447807838233444 1101 94-95 -1.8203335935866427 1101 96-97 -1.7910434983955597 1101 98-99 -1.8270651074878543 1101 100 -2.035480031865262 1102 1 -0.46966201161521326 1102 2 -0.43471723511403937 1102 3 -0.47554125538459857 1102 4 -0.27472410227560573 1102 5 -0.3066141869339347 1102 6 -0.28190878060666336 1102 7 -0.24526359694567645 1102 8 -0.36480566630874733 1102 9 -0.4886407964645727 1102 10-11 -0.501399877705687 1102 12-13 -0.5703581250289105 1102 14-15 -0.6719304784665852 1102 16-17 -0.7500390495844584 1102 18-19 -0.7454323626532613 1102 20-21 -0.7532730302315329 1102 22-23 -0.7653226842882432 1102 24-25 -0.7510489082030674 1102 26-27 -0.8476109361228694 1102 28-29 -0.8822380308983497 1102 30-31 -0.8894668280255544 1102 32-33 -0.9546051307951231 1102 34-35 -1.0495095055240995 1102 36-37 -0.9818406563775923 1102 38-39 -1.0964173338697236 1102 40-41 -1.0943180502824887 1102 42-43 -1.1097237726739735 1102 44-45 -1.3349511976133286 1102 46-47 -1.2592362381271514 1102 48-49 -1.2603249464772404 1102 50-51 -1.3141846351747688 1102 52-53 -1.0915235383741262 1102 54-55 -0.9429524903288566 1102 56-57 -0.9120221954404002 1102 58-59 -0.9307634549513537 1102 60-61 -0.8938835708959942 1102 62-63 -0.9906219494170116 1102 64-65 -0.9724299885450733 1102 66-67 -1.0357305623888138 1102 68-69 -1.1638788362760764 1102 70-71 -1.1329328795304825 1102 72-73 -1.1489192558109487 1102 74-75 -1.1425562051728662 1102 76-77 -1.1965398899944972 1102 78-79 -1.2726015381095017 1102 80-81 -1.3332678316296303 1102 82-83 -1.3014231335830253 1102 84-85 -1.283785490841968 1102 86-87 -1.3613391932333094 1102 88-89 -1.3439364250876586 1102 90-91 -1.3739848324924466 1102 92-93 -1.437227655239802 1102 94-95 -1.5730186927199519 1102 96-97 -1.5941167538018064 1102 98-99 -1.580471219184087 1102 100 -1.7588613143691951 1103 1 -0.5895854078103113 1103 2 -0.4949030775679901 1103 3 -0.5750521502813477 1103 4 -0.49907658573374647 1103 5 -0.38485260476119976 1103 6 -0.44510113906852666 1103 7 -0.449135530040202 1103 8 -0.47265390919445593 1103 9 -0.7528327780794513 1103 10-11 -0.7163229985728279 1103 12-13 -0.6692428445623761 1103 14-15 -0.8833281292797466 1103 16-17 -0.8436457909698305 1103 18-19 -0.7514139603070049 1103 20-21 -0.8489566382229796 1103 22-23 -0.9471600951720234 1103 24-25 -1.000825318417192 1103 26-27 -0.9464633600788588 1103 28-29 -0.9615995518986935 1103 30-31 -0.9941273016835837 1103 32-33 -1.0497825137650807 1103 34-35 -1.0891425077157635 1103 36-37 -1.0261212531424562 1103 38-39 -1.066016071432344 1103 40-41 -1.1525707903828177 1103 42-43 -1.2685738765678707 1103 44-45 -1.2293363733299785 1103 46-47 -1.2848125412895826 1103 48-49 -1.366608484945118 1103 50-51 -1.5506443391672065 1103 52-53 -1.3183162952394483 1103 54-55 -1.0300909821162563 1103 56-57 -0.9080806670075035 1103 58-59 -0.924686438545983 1103 60-61 -1.00336401269427 1103 62-63 -1.0241468466322488 1103 64-65 -0.9173256105435428 1103 66-67 -1.0228521299887419 1103 68-69 -0.9876370419624934 1103 70-71 -1.0284597528436095 1103 72-73 -1.055683783079374 1103 74-75 -1.0581411439769894 1103 76-77 -1.1602073653337257 1103 78-79 -1.2354349067613661 1103 80-81 -1.1286668340290298 1103 82-83 -1.120881329926565 1103 84-85 -1.2505881816964326 1103 86-87 -1.3935813362086371 1103 88-89 -1.4909274624450966 1103 90-91 -1.5096611632137744 1103 92-93 -1.5804510383339903 1103 94-95 -1.6475699668750856 1103 96-97 -1.5873051598069274 1103 98-99 -1.6398165610787707 1103 100 -1.6456020290318314 1104 1 -0.5785366231766211 1104 2 -0.5861620970468628 1104 3 -0.5962234446273769 1104 4 -0.5297840774748721 1104 5 -0.49091539437608134 1104 6 -0.48718690818464694 1104 7 -0.6460902780614006 1104 8 -0.538448374701801 1104 9 -0.6972630574153911 1104 10-11 -0.6946177412236025 1104 12-13 -0.6707030824869236 1104 14-15 -0.9069019492030392 1104 16-17 -0.9374914824365206 1104 18-19 -0.9491474813588141 1104 20-21 -0.9141266072075567 1104 22-23 -0.9219067860794965 1104 24-25 -0.9577704711170583 1104 26-27 -0.8944416911580859 1104 28-29 -0.9247727332835183 1104 30-31 -1.0681864921291577 1104 32-33 -1.040767961887532 1104 34-35 -1.154932354165993 1104 36-37 -1.0891378176971642 1104 38-39 -1.1471741367987747 1104 40-41 -1.150334242924778 1104 42-43 -0.9987424167241059 1104 44-45 -1.1052447878971279 1104 46-47 -1.2671428001785046 1104 48-49 -1.169101605629379 1104 50-51 -1.1943512125705524 1104 52-53 -1.031850071016862 1104 54-55 -0.9987097239097338 1104 56-57 -1.018950663336895 1104 58-59 -1.0298783819249664 1104 60-61 -1.0134859065323312 1104 62-63 -1.0824575157919796 1104 64-65 -0.9920123768833093 1104 66-67 -1.0288753804746662 1104 68-69 -1.1089559613741145 1104 70-71 -1.0670439687247892 1104 72-73 -1.0495321228054841 1104 74-75 -1.060261907069858 1104 76-77 -1.1128069090940578 1104 78-79 -1.1231835044156213 1104 80-81 -1.2184834759375498 1104 82-83 -1.2218933345480885 1104 84-85 -1.120489050571873 1104 86-87 -1.218925240408165 1104 88-89 -1.3034325434079328 1104 90-91 -1.2989681075658375 1104 92-93 -1.4016987626785138 1104 94-95 -1.4278906895666061 1104 96-97 -1.522891176504512 1104 98-99 -1.4872463329343013 1104 100 -1.4412691071025172 1105 1 -0.6509428365753394 1105 2 -0.6074749412383298 1105 3 -0.676562422507569 1105 4 -0.4703171465323308 1105 5 -0.3950148088365566 1105 6 -0.4036996315905128 1105 7 -0.38402512369174957 1105 8 -0.551634091237446 1105 9 -0.8053657323311896 1105 10-11 -0.7120216566519488 1105 12-13 -0.7608196889177137 1105 14-15 -1.0168338888516715 1105 16-17 -0.8980211795401019 1105 18-19 -0.8881199671668014 1105 20-21 -0.8618868597342342 1105 22-23 -0.8873552011076313 1105 24-25 -0.9428934203780273 1105 26-27 -0.9453194790564581 1105 28-29 -0.9832058053477937 1105 30-31 -1.0409242601825355 1105 32-33 -1.0627284527255014 1105 34-35 -1.1172007740461964 1105 36-37 -1.1565891790678577 1105 38-39 -1.1013349260843626 1105 40-41 -1.1298389929353263 1105 42-43 -1.101159466810472 1105 44-45 -1.1773963161883145 1105 46-47 -1.2685668111966066 1105 48-49 -1.267655790974331 1105 50-51 -1.2340669708968974 1105 52-53 -1.001914438484782 1105 54-55 -0.9179731780883955 1105 56-57 -0.8992168988713161 1105 58-59 -0.8764356159994762 1105 60-61 -0.884349741250432 1105 62-63 -0.9977546299130822 1105 64-65 -0.9474725512293816 1105 66-67 -0.9387760016505311 1105 68-69 -0.96750735554307 1105 70-71 -0.9833602788542422 1105 72-73 -0.8343882112087186 1105 74-75 -0.9366513742900082 1105 76-77 -0.9239906950578671 1105 78-79 -1.0259326609125168 1105 80-81 -0.9826901517380513 1105 82-83 -1.0796108011915742 1105 84-85 -1.0626005041765758 1105 86-87 -1.1904673602528426 1105 88-89 -1.1846537552926115 1105 90-91 -1.1652970912068987 1105 92-93 -1.2155957785769473 1105 94-95 -1.288791247602699 1105 96-97 -1.315046632217566 1105 98-99 -1.3081076552227948 1105 100 -1.203105189985493 1106 1 -0.5975562915216663 1106 2 -0.5703344194561382 1106 3 -0.683447449164845 1106 4 -0.5024992567230697 1106 5 -0.44040916738466507 1106 6 -0.5315347125897603 1106 7 -0.48196333439427974 1106 8 -0.5160592124522978 1106 9 -0.6611759843537399 1106 10-11 -0.5380475550314614 1106 12-13 -0.758611572642657 1106 14-15 -0.9334123216273085 1106 16-17 -0.9731692937737222 1106 18-19 -0.9569967743240824 1106 20-21 -0.8143201803298794 1106 22-23 -0.8724658828898129 1106 24-25 -0.7539467070312256 1106 26-27 -0.9093972589638852 1106 28-29 -0.9444680366507896 1106 30-31 -1.0335039620776172 1106 32-33 -1.0476637788705503 1106 34-35 -1.0951245572668569 1106 36-37 -1.032324401880679 1106 38-39 -1.0500762887420123 1106 40-41 -1.069348976956654 1106 42-43 -1.1104086677091871 1106 44-45 -1.1310434284184723 1106 46-47 -1.0733115199463015 1106 48-49 -1.0966994359680058 1106 50-51 -1.1121157681507086 1106 52-53 -1.0937478620639283 1106 54-55 -0.992179881190502 1106 56-57 -0.8945870338966628 1106 58-59 -0.8915285070393608 1106 60-61 -0.8569897350376223 1106 62-63 -0.9329143556105208 1106 64-65 -0.9762005795486459 1106 66-67 -0.9823073553524537 1106 68-69 -1.042277849065833 1106 70-71 -0.8908959337739333 1106 72-73 -0.9448391590445837 1106 74-75 -0.9472321815342113 1106 76-77 -1.040381033386442 1106 78-79 -1.0432191991659003 1106 80-81 -0.9273173549418878 1106 82-83 -0.989031323826822 1106 84-85 -0.9611483737338453 1106 86-87 -1.0539591247245532 1106 88-89 -1.1204221204717406 1106 90-91 -1.1588351043656644 1106 92-93 -1.206854872625243 1106 94-95 -1.3188937529399887 1106 96-97 -1.2646837689435237 1106 98-99 -1.2883233344411202 1106 100 -1.4429364408246315 1107 1 -0.7126811124935237 1107 2 -0.646153872930693 1107 3 -0.5967138900953337 1107 4 -0.4987228813462039 1107 5 -0.4739642603334886 1107 6 -0.45937243704184283 1107 7 -0.489144276587659 1107 8 -0.4852374426303001 1107 9 -0.7434506242926133 1107 10-11 -0.7636193515295631 1107 12-13 -0.6936117482741508 1107 14-15 -0.9419945380437511 1107 16-17 -0.9678073305184824 1107 18-19 -0.9092522638024292 1107 20-21 -0.884884921743982 1107 22-23 -0.9989965445035907 1107 24-25 -1.0602281011608952 1107 26-27 -1.051308152822152 1107 28-29 -1.0820791401401522 1107 30-31 -1.0170502734517086 1107 32-33 -1.1569723972074044 1107 34-35 -1.135421463965443 1107 36-37 -1.1519771187673484 1107 38-39 -1.1270707500537043 1107 40-41 -1.0822129732292964 1107 42-43 -1.0719217429768904 1107 44-45 -1.1375729931638645 1107 46-47 -0.981242657156919 1107 48-49 -0.9686064327953687 1107 50-51 -1.0894905047863048 1107 52-53 -1.017830204073455 1107 54-55 -0.8725511065217333 1107 56-57 -0.8933812408409381 1107 58-59 -0.962106487091603 1107 60-61 -0.889143122969152 1107 62-63 -0.8896302057232859 1107 64-65 -0.8657097624247712 1107 66-67 -0.8981814931840759 1107 68-69 -0.8705237510528505 1107 70-71 -0.8302517041963924 1107 72-73 -0.791617911742847 1107 74-75 -0.7818824031281366 1107 76-77 -0.8811827812180546 1107 78-79 -0.9011752893039606 1107 80-81 -0.984136910481535 1107 82-83 -0.8203778010235574 1107 84-85 -0.8304394057246292 1107 86-87 -0.8744558834950276 1107 88-89 -0.9095493048470331 1107 90-91 -1.0365803074718904 1107 92-93 -1.0466749232468793 1107 94-95 -1.0386176834270309 1107 96-97 -1.105775109913182 1107 98-99 -1.071563483668239 1107 100 -1.1575582598234533 1108 1 -0.6427734314134277 1108 2 -0.652525702888731 1108 3 -0.7330933828886188 1108 4 -0.4945491904138919 1108 5 -0.4925163493683371 1108 6 -0.4940982170089967 1108 7 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0.7341529633971788 2311 7 0.7849745383745415 2311 8 0.7200501584418149 2311 9 0.9480116792566307 2311 10-11 0.9159798591373516 2311 12-13 1.005597910055812 2311 14-15 1.2693384418809401 2311 16-17 1.2497450900819018 2311 18-19 1.1695241085439605 2311 20-21 1.2231444337653414 2311 22-23 1.242021039727824 2311 24-25 1.3062349789997967 2311 26-27 1.3121905308382509 2311 28-29 1.2918685042796483 2311 30-31 1.3072219542101564 2311 32-33 1.348050557544319 2311 34-35 1.3913631252662668 2311 36-37 1.4284110820639668 2311 38-39 1.454829570421225 2311 40-41 1.477466335326973 2311 42-43 1.5050832290845904 2311 44-45 1.573605067905632 2311 46-47 1.588128728658134 2311 48-49 1.573315544044526 2311 50-51 1.5733800380523206 2311 52-53 1.433695820613245 2311 54-55 1.3705954420085504 2311 56-57 1.2916927510669751 2311 58-59 1.24486946726973 2311 60-61 1.2580280547005813 2311 62-63 1.286341893283307 2311 64-65 1.2615962591985124 2311 66-67 1.2952234618793668 2311 68-69 1.3185186379465108 2311 70-71 1.2970318192285646 2311 72-73 1.2368151109324614 2311 74-75 1.2798819894991809 2311 76-77 1.248200538190062 2311 78-79 1.2463684735916587 2311 80-81 1.243790218755077 2311 82-83 1.3152814466167904 2311 84-85 1.3477142452703568 2311 86-87 1.3616493139384325 2311 88-89 1.4390453108351942 2311 90-91 1.5125467140208144 2311 92-93 1.5091981756183799 2311 94-95 1.522733323296741 2311 96-97 1.5599509626208459 2311 98-99 1.6783260223409826 2311 100 1.8150876291586293 2312 1 0.6784684133559757 2312 2 0.7343489377811849 2312 3 0.7374697967139312 2312 4 0.5214368113358887 2312 5 0.6069442180607325 2312 6 0.6325749488452672 2312 7 0.4890146317097219 2312 8 0.6726656355768839 2312 9 0.8349882490091503 2312 10-11 0.7983899806372463 2312 12-13 0.905568670265481 2312 14-15 1.1550540636396605 2312 16-17 1.1575408658994917 2312 18-19 1.1593363465293294 2312 20-21 1.1806371466181602 2312 22-23 1.2031718769724549 2312 24-25 1.258219219360818 2312 26-27 1.2678469240576007 2312 28-29 1.2465785563834118 2312 30-31 1.2244435771732824 2312 32-33 1.2992793500598196 2312 34-35 1.3043578021320457 2312 36-37 1.3308967461374621 2312 38-39 1.3574051703412948 2312 40-41 1.4417766286451794 2312 42-43 1.445531017956725 2312 44-45 1.4018993572750915 2312 46-47 1.487777364765627 2312 48-49 1.5216063566978946 2312 50-51 1.4765672581082683 2312 52-53 1.3507909883924256 2312 54-55 1.2775462376320874 2312 56-57 1.1981718408859194 2312 58-59 1.2133996924520432 2312 60-61 1.2248503002667306 2312 62-63 1.133719921487824 2312 64-65 1.169857257215014 2312 66-67 1.167323379051723 2312 68-69 1.2008738616018917 2312 70-71 1.1542442280649468 2312 72-73 1.2368716448786614 2312 74-75 1.2509920651495392 2312 76-77 1.2167611252189303 2312 78-79 1.1852140269740374 2312 80-81 1.1701663447150104 2312 82-83 1.2251924366047824 2312 84-85 1.3129864447796926 2312 86-87 1.3017384427888992 2312 88-89 1.2320719580123516 2312 90-91 1.3107547957551482 2312 92-93 1.435975693701863 2312 94-95 1.4159132444598548 2312 96-97 1.5082571303096657 2312 98-99 1.1787195987625445 2312 100 1.4128131161683406 2313 1 0.6363258275034056 2313 2 0.6371893853847581 2313 3 0.6379793530517475 2313 4 0.5433324513654085 2313 5 0.43385662557415827 2313 6 0.5405612715681727 2313 7 0.6134452074256274 2313 8 0.5792142676501086 2313 9 0.7130360069437458 2313 10-11 0.7699984355365004 2313 12-13 0.8606996265044131 2313 14-15 1.0124548501653692 2313 16-17 1.0168668117196091 2313 18-19 0.9816852912063183 2313 20-21 1.037954147479958 2313 22-23 1.0733957132612986 2313 24-25 1.0220908240946258 2313 26-27 0.9969695818849473 2313 28-29 1.108693809976515 2313 30-31 1.146235345061939 2313 32-33 1.1777966877841521 2313 34-35 1.2640337856010433 2313 36-37 1.2715158756933889 2313 38-39 1.2947988544076594 2313 40-41 1.2763618367915655 2313 42-43 1.3267247141790222 2313 44-45 1.2978485189539768 2313 46-47 1.3732076673712754 2313 48-49 1.3753764063487566 2313 50-51 1.3838275852726056 2313 52-53 1.2574535942359262 2313 54-55 1.122631906403221 2313 56-57 1.0093574924317252 2313 58-59 1.045911587611677 2313 60-61 1.035077953198531 2313 62-63 1.0233587869097533 2313 64-65 1.096069318709155 2313 66-67 1.0243693770308653 2313 68-69 1.035989471645017 2313 70-71 1.0256974798951717 2313 72-73 0.9952880406247289 2313 74-75 0.9858207687463363 2313 76-77 0.9198108405764529 2313 78-79 1.0731006551610847 2313 80-81 1.0774697006801368 2313 82-83 1.0960611528458735 2313 84-85 1.1966926864241536 2313 86-87 1.1999429060316835 2313 88-89 1.2423220224414244 2313 90-91 1.30535161838662 2313 92-93 1.3868685200852156 2313 94-95 1.4185814866197468 2313 96-97 1.4017072030196331 2313 98-99 1.3935129386940552 2313 100 1.555677546515799 2314 1 0.6080261701335061 2314 2 0.5815386225406485 2314 3 0.6579007575074911 2314 4 0.49849009497485497 2314 5 0.515519876005051 2314 6 0.4387619077354543 2314 7 0.515298653938693 2314 8 0.5416688631266737 2314 9 0.7167119306982386 2314 10-11 0.6918898697507814 2314 12-13 0.7605345538368482 2314 14-15 0.9303220159434105 2314 16-17 0.9093917009930124 2314 18-19 0.8608665420986057 2314 20-21 0.9191907133944213 2314 22-23 0.9710243936646421 2314 24-25 0.9444693404746829 2314 26-27 1.0131014138829642 2314 28-29 0.990656949886116 2314 30-31 1.0399239255075088 2314 32-33 1.041343630837268 2314 34-35 1.0792914102054354 2314 36-37 1.0519749932269704 2314 38-39 1.1089584313723506 2314 40-41 1.0951039762624148 2314 42-43 1.1956233497807105 2314 44-45 1.2567773552468324 2314 46-47 1.2530494080264276 2314 48-49 1.2015798838995266 2314 50-51 1.2829629042534023 2314 52-53 1.2136036989959749 2314 54-55 1.1363655174514022 2314 56-57 1.0411521999858167 2314 58-59 1.0677697173638236 2314 60-61 1.0285490529554622 2314 62-63 1.0478882729828527 2314 64-65 1.0787763671519528 2314 66-67 1.0829764734301008 2314 68-69 1.1009864974916397 2314 70-71 1.09529587316797 2314 72-73 1.0971635884923643 2314 74-75 1.1559091963551467 2314 76-77 1.1572451877115242 2314 78-79 1.1601956499643507 2314 80-81 1.1274635785821054 2314 82-83 1.1473380789819352 2314 84-85 1.2475993002892416 2314 86-87 1.3027858677467528 2314 88-89 1.314391751354428 2314 90-91 1.4568169535412103 2314 92-93 1.478818855021217 2314 94-95 1.4715199495384574 2314 96-97 1.4669137945863469 2314 98-99 1.5955461088101508 2314 100 1.7005085401099116 2315 1 0.46564146529881967 2315 2 0.44900444291025465 2315 3 0.5121181387872618 2315 4 0.2719988973887766 2315 5 0.32584468900491714 2315 6 0.40744951518639283 2315 7 0.4097423233348252 2315 8 0.43426663199000615 2315 9 0.5898102684563113 2315 10-11 0.5331747949590877 2315 12-13 0.5085694026077476 2315 14-15 0.7111973995923151 2315 16-17 0.6965074881979731 2315 18-19 0.5428096267277525 2315 20-21 0.6841629202549129 2315 22-23 0.5523066707138682 2315 24-25 0.7195966108325038 2315 26-27 0.8128617847254773 2315 28-29 0.832507428309043 2315 30-31 0.8700351440115455 2315 32-33 0.9227476856115615 2315 34-35 0.9053709736016913 2315 36-37 1.0095204505064004 2315 38-39 1.0009309172502299 2315 40-41 1.0196377081283785 2315 42-43 1.1006249662437426 2315 44-45 1.1091320657119965 2315 46-47 1.1689659509174106 2315 48-49 1.1737421767875915 2315 50-51 1.08861215015461 2315 52-53 1.1333941978690447 2315 54-55 1.0075031523863842 2315 56-57 0.9559381167918559 2315 58-59 0.9484950038578788 2315 60-61 1.0126838233675883 2315 62-63 0.9336562558207717 2315 64-65 1.0176428840625817 2315 66-67 1.0145491917110903 2315 68-69 1.033324508682405 2315 70-71 1.0064123353611052 2315 72-73 0.7116402888694466 2315 74-75 0.9411944749297803 2315 76-77 1.05269860350257 2315 78-79 1.027130251295283 2315 80-81 0.7806033178428642 2315 82-83 0.7562597853654154 2315 84-85 1.0727135804661465 2315 86-87 1.0870010436196047 2315 88-89 1.190849053339793 2315 90-91 1.2710426009200333 2315 92-93 1.2979766986678953 2315 94-95 1.2994663606563783 2315 96-97 1.153744779384077 2315 98-99 0.579720288340301 2315 100 1.1647540904508311 2316 1 0.30048525584525976 2316 2 0.2794812514340137 2316 3 0.3205818863655985 2316 4 0.15689148334256942 2316 5 0.10206234963141014 2316 6 0.1514460039036436 2316 7 0.07573190371501681 2316 8 0.21144830196833198 2316 9 0.25692421256135844 2316 10-11 0.23046646360888445 2316 12-13 0.0204936716388886 2316 14-15 0.04729170178695341 2316 16-17 0.22874323871810986 2316 18-19 0.30689362022004474 2316 20-21 0.27737717182483124 2316 22-23 0.28894201630247807 2316 24-25 0.37044308078070287 2316 26-27 0.40926852445144135 2316 28-29 0.3579684911052965 2316 30-31 0.3156439712207586 2316 32-33 0.4677587897742299 2316 34-35 0.45043805383599533 2316 36-37 0.4143739484004172 2316 38-39 0.5694104602206096 2316 40-41 0.6603329950879981 2316 42-43 0.5343419098384814 2316 44-45 0.4620263079780855 2316 46-47 0.32533869733731535 2316 48-49 0.2876471503184632 2316 50-51 0.37608093909729945 2316 52-53 0.5136097398891692 2316 54-55 0.24920491187675253 2316 56-57 0.13735274697108935 2316 58-59 0.23206859283664727 2316 60-61 0.2822237455846448 2316 62-63 0.4210365798073852 2316 64-65 0.3860761562395538 2316 66-67 0.3438138337477312 2316 68-69 0.3378619745742526 2316 70-71 0.1229605176183668 2316 72-73 -0.020421449530047653 2316 74-75 0.27004658056128505 2316 76-77 0.47690571952016825 2316 78-79 0.49274821677925473 2316 80-81 0.646376971302761 2316 82-83 0.6855939991406181 2316 84-85 0.7072724310797689 2316 86-87 0.7292734486372119 2316 88-89 0.7891726475954322 2316 90-91 0.6021718359082016 2316 92-93 0.6104870092326564 2316 94-95 0.7877831405237039 2316 96-97 0.7864659904041424 2316 98-99 0.7071269386700934 2316 100 0.500501692791854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 36.0 1 552.0 2 193.0 3 197.0 4 144.0 5 113.0 6 133.0 7 479.0 8 1590.0 9 3330.0 10 5884.0 11 10905.0 12 17857.0 13 23375.0 14 27185.0 15 31551.0 16 36553.0 17 42716.0 18 49393.0 19 58324.0 20 68592.0 21 82449.0 22 89677.0 23 92169.0 24 93661.0 25 99208.0 26 109235.0 27 126310.0 28 147528.0 29 174001.0 30 208489.0 31 255386.0 32 320529.0 33 415348.0 34 580064.0 35 796366.0 36 1178907.0 37 1550349.0 38 1263125.0 39 330195.0 40 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84647693977087 19.978662270093622 12.74370700463713 23.431153785498378 2 15.735765877229824 19.18113446960137 41.21033726502072 23.872762388148086 3 19.623042764492972 25.332738629884787 28.272371606351825 26.77184699927041 4 12.680276936637513 15.703967461561305 38.48742382397092 33.128331777830255 5 13.469740841174001 37.87870235078966 34.41499715465894 14.236559653377393 6 31.296866244817174 37.125908608499955 17.344727548966283 14.232497597716588 7 28.254667754080785 31.57777249274243 22.055019631251742 18.11254012192504 8 26.56193594780074 35.60110469774042 19.209277241006532 18.627682113452313 9 26.222525382831087 15.614016652734497 21.084214454173974 37.07924351026045 10-11 26.287508601728167 25.929807002795986 26.704221916383 21.078462479092856 12-13 26.070357932786255 23.384412816372457 27.621080102538993 22.924149148302295 14-15 23.3834643962628 24.703391143991997 25.193150818600035 26.719993641145166 16-17 22.270141023681813 27.704101749428922 26.325484022980923 23.700273203908345 18-19 21.795054881367886 27.563915689076783 28.513488852755493 22.12754057679984 20-21 23.12818286937568 26.697549934271734 28.153402174168047 22.020865022184534 22-23 23.4700129079854 26.43869876988745 27.816298415349078 22.27498990677807 24-25 22.8857400215252 26.377991341067087 28.527459709605797 22.20880892780192 26-27 22.862474818934245 26.60876727771051 27.632516347979397 22.89624155537585 28-29 22.183135476913407 26.870073202060812 28.268861512286154 22.677929808739634 30-31 22.906221565443264 26.7015986553078 28.12435773482662 22.267822044422317 32-33 23.0811193016903 26.84649593705698 27.476271102823635 22.596113658429083 34-35 23.109454100055974 26.735713752827884 28.020158666085997 22.134673481030145 36-37 22.821956521122914 26.3796947635839 27.656238512683384 23.142110202609803 38-39 22.95839968127657 26.622597339014003 28.06387278274537 22.35513019696406 40-41 22.757979515794986 25.83319160125882 28.57764927403022 22.83117960891597 42-43 22.698991047787576 26.01161061956297 28.07141624551848 23.217982087130977 44-45 22.893581808813313 26.654946565240976 27.889773700782484 22.561697925163227 46-47 22.83483600220766 26.636441362554102 27.314139927401115 23.214582707837124 48-49 23.358167360957797 26.562311874851464 27.32527810853622 22.754242655654522 50-51 23.02202297870585 26.37219462479175 27.466389417737442 23.139392978764956 52-53 22.716428152371673 26.86407719319326 27.17629741799424 23.243197236440828 54-55 22.56784727535717 26.777612940059154 27.230498121221974 23.424041663361702 56-57 23.312002578799916 26.451778335643787 26.925241946467942 23.31097713908835 58-59 22.555583873852544 26.767981155094954 27.528304305403285 23.14813066564922 60-61 22.5846513483513 27.261203542288808 27.029266673119913 23.124878436239975 62-63 22.881232394504238 26.71349704666442 26.738369780472794 23.666900778358553 64-65 22.38395867138161 26.78221102511436 27.088921232157926 23.7449090713461 66-67 22.70749158759406 26.556374263143567 26.994870677636197 23.741263471626176 68-69 22.395951146709827 26.56872073478028 27.292493735419793 23.742834383090102 70-71 22.349502706115274 26.257868720157045 27.351009840073694 24.04161873365399 72-73 22.854438626547307 26.27879255845378 27.467479429577974 23.39928938542094 74-75 23.032146637050527 26.53457616904264 27.117447926279066 23.31582926762777 76-77 22.793668744650976 26.457840169641777 26.80854261486122 23.93994847084603 78-79 22.728407077287045 26.46462158582439 26.807602647354727 23.99936868953384 80-81 22.350809557408738 26.825435721487327 27.3064134036621 23.517341317441833 82-83 23.055006290539392 26.09370379758485 28.102117035936196 22.749172875939564 84-85 23.100935200401334 26.366782532754566 27.198639976037605 23.33364229080649 86-87 22.179970340662656 26.851481812021362 27.54700056970388 23.42154727761211 88-89 22.99989578832873 25.893952804174447 27.42995323260021 23.676198174896616 90-91 22.75125877256889 26.08458768323126 27.37827321878133 23.78588032541852 92-93 22.9615040763767 26.11787357780336 27.429265287256943 23.491357058562997 94-95 22.16088155419697 26.05777270068942 27.992660429616002 23.788685315497613 96-97 22.464124258705926 25.94979938341317 27.602697677465027 23.983378680415882 98-99 22.002907099474672 26.262260179371655 27.49544333266989 24.239389388483783 100 22.67451524782066 25.787650668174045 26.83120894253807 24.706625141467224 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 574.0 1 437.0 2 463.0 3 658.5 4 590.0 5 586.0 6 851.0 7 1467.5 8 2431.0 9 3071.5 10 2945.0 11 2825.0 12 3230.0 13 3849.0 14 4523.0 15 5314.0 16 6157.0 17 6866.5 18 7656.5 19 8336.0 20 8993.5 21 9990.0 22 11252.0 23 13045.0 24 15290.5 25 18138.0 26 22289.0 27 27335.0 28 33581.5 29 44868.5 30 61751.5 31 78850.0 32 101090.0 33 127057.0 34 150247.5 35 173311.0 36 197313.0 37 230603.0 38 248065.5 39 246153.5 40 239532.0 41 228787.0 42 229313.0 43 242066.5 44 261872.5 45 297002.0 46 332273.0 47 356747.0 48 375091.0 49 376533.0 50 365778.0 51 374482.0 52 376617.0 53 357388.0 54 337278.0 55 296870.5 56 260301.5 57 219180.5 58 179530.0 59 166324.5 60 140359.0 61 104063.5 62 79794.0 63 65542.0 64 49053.0 65 28933.0 66 18287.0 67 13163.0 68 9881.5 69 7221.0 70 5561.5 71 4243.5 72 3070.0 73 2312.0 74 1761.0 75 1266.5 76 892.0 77 597.5 78 401.0 79 288.0 80 203.5 81 147.0 82 112.0 83 97.5 84 78.5 85 55.5 86 43.5 87 33.5 88 31.5 89 27.5 90 20.5 91 18.0 92 17.5 93 15.0 94 14.0 95 9.0 96 4.5 97 5.0 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05665452497568546 2 0.13389425091635498 3 0.01685888163388852 4 0.01653328091563746 5 0.019523983809202804 6 0.05326586564870215 7 0.05805340213557894 8 0.04974455417724617 9 0.040953334784467395 10-11 0.04019359977521491 12-13 0.04124878728806559 14-15 0.01886072308683952 16-17 0.017944217361392074 18-19 0.017871861646225173 20-21 0.02160421062025128 22-23 0.025903346029751466 24-25 0.0251134628058461 26-27 0.020036503458301703 28-29 0.018649685584269386 30-31 0.01684682234802737 32-33 0.023467370285799045 34-35 0.023340747784256963 36-37 0.023774882075258384 38-39 0.02160421062025128 40-41 0.025023018161887473 42-43 0.03167371431431202 44-45 0.04186984050991484 46-47 0.047579912365169647 48-49 0.04021771834693721 50-51 0.02824284748681468 52-53 0.04032625191968757 54-55 0.03699185937907943 56-57 0.03904796761840561 58-59 0.04404654160785252 60-61 0.02366634850250803 62-63 0.026078205674738147 64-65 0.0256742195983896 66-67 0.0322405007497861 68-69 0.03981976191351924 70-71 0.04164674372148356 72-73 0.045403211267231965 74-75 0.027627823907896 76-77 0.020723882752387287 78-79 0.020187244531566086 80-81 0.024884336374484242 82-83 0.0209891870413326 84-85 0.012547686938527187 86-87 0.014658061964228539 88-89 0.01865571522719996 90-91 0.021091690971152377 92-93 0.020126948102260334 94-95 0.02365428921664688 96-97 0.02470344708656698 98-99 0.02791724676856361 100 0.029653783932569296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8292365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.61167026383414 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8459521927366 49.651453241874606 2 13.876286201815146 17.931400571165433 3 4.595963435402552 8.908586220986042 4 1.9177382190326935 4.9563307784197175 5 0.926316892317384 2.992544080311519 6 0.5405829967607981 2.0956782202166404 7 0.3234362001104382 1.4628427179046177 8 0.22055815794870107 1.1400504780304097 9 0.1534718090495941 0.8924462928995841 >10 0.5619719136280223 5.9353759104819135 >50 0.023520302037335652 1.0454493671147587 >100 0.012956745294487312 1.5728486050801307 >500 9.09782415454756E-4 0.38733903950902326 >1k 2.6333328371329985E-4 0.3205822907304077 >5k 2.3939388994407322E-5 0.1473137946002349 >10k+ 4.7878777988814645E-5 0.5597583906749161 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19981 0.2409565907916499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16208 0.19545690523752873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9524 0.1148526385415982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8088928791725882E-5 0.0 18-19 0.0 0.0 0.0 3.014821465287647E-5 0.0 20-21 0.0 0.0 0.0 6.029642930575294E-5 6.029642930575294E-6 22-23 0.0 0.0 0.0 2.5324500308416235E-4 1.2059285861150588E-5 24-25 0.0 1.2059285861150588E-5 0.0 6.210532218492553E-4 1.8088928791725882E-5 26-27 0.0 1.2059285861150588E-5 0.0 0.0011938693002539083 2.4118571722301176E-5 28-29 0.0 1.2059285861150588E-5 0.0 0.003557489329039424 2.4118571722301176E-5 30-31 0.0 1.2059285861150588E-5 0.0 0.015315293043661247 2.4118571722301176E-5 32-33 0.0 1.2059285861150588E-5 0.0 0.04369079267494858 2.4118571722301176E-5 34-35 0.0 1.2059285861150588E-5 0.0 0.08691127320131228 2.4118571722301176E-5 36-37 0.0 1.2059285861150588E-5 0.0 0.1601714348078021 2.4118571722301176E-5 38-39 0.0 1.2059285861150588E-5 0.0 0.26577460109389783 2.4118571722301176E-5 40-41 0.0 1.2059285861150588E-5 0.0 0.3750799681393668 3.6177857583451765E-5 42-43 0.0 1.2059285861150588E-5 0.0 0.48266085730669117 3.6177857583451765E-5 44-45 0.0 1.2059285861150588E-5 0.0 0.6101636867166363 3.6177857583451765E-5 46-47 0.0 1.2059285861150588E-5 0.0 0.764221063592835 3.6177857583451765E-5 48-49 0.0 1.2059285861150588E-5 0.0 0.9321647081381488 3.6177857583451765E-5 50-51 0.0 1.2059285861150588E-5 0.0 1.1037804052281828 3.6177857583451765E-5 52-53 0.0 1.2059285861150588E-5 0.0 1.2672259361472873 3.6177857583451765E-5 54-55 0.0 2.4118571722301176E-5 0.0 1.4414404093403994 3.6177857583451765E-5 56-57 0.0 2.4118571722301176E-5 0.0 1.6495957425897196 3.6177857583451765E-5 58-59 0.0 2.4118571722301176E-5 0.0 1.8866149765477038 3.6177857583451765E-5 60-61 0.0 2.4118571722301176E-5 0.0 2.1267696248295875 3.6177857583451765E-5 62-63 1.2059285861150588E-5 2.4118571722301176E-5 0.0 2.365525395951577 3.6177857583451765E-5 64-65 1.2059285861150588E-5 2.4118571722301176E-5 0.0 2.6077663006874396 3.6177857583451765E-5 66-67 1.2059285861150588E-5 2.4118571722301176E-5 0.0 2.8810116293723205 3.6177857583451765E-5 68-69 1.2059285861150588E-5 2.4118571722301176E-5 0.0 3.1792860058620187 3.6177857583451765E-5 70-71 1.2059285861150588E-5 2.4118571722301176E-5 0.0 3.5027401712298 3.6177857583451765E-5 72-73 1.2059285861150588E-5 2.4118571722301176E-5 0.0 3.8302764048615803 6.029642930575294E-5 74-75 1.2059285861150588E-5 2.4118571722301176E-5 0.0 4.1414783357944325 6.632607223632824E-5 76-77 1.2059285861150588E-5 2.4118571722301176E-5 0.0 4.486838193928993 7.235571516690353E-5 78-79 1.2059285861150588E-5 2.4118571722301176E-5 0.0 4.863522047087894 7.235571516690353E-5 80-81 1.2059285861150588E-5 2.4118571722301176E-5 0.0 5.265361570553154 7.235571516690353E-5 82-83 1.2059285861150588E-5 2.4118571722301176E-5 0.0 5.666525774010189 7.235571516690353E-5 84-85 1.2059285861150588E-5 2.4118571722301176E-5 0.0 6.055039786598877 7.235571516690353E-5 86-87 1.2059285861150588E-5 2.4118571722301176E-5 0.0 6.461552283335333 7.838535809747882E-5 88 1.2059285861150588E-5 2.4118571722301176E-5 0.0 6.794286069173269 8.441500102805412E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13075 0.0 46.935837 1 GTATCAA 22920 0.0 38.786983 1 GTACTAG 7570 0.0 36.182266 1 GCATCGT 7480 0.0 32.928654 8 CAACGCA 25165 0.0 32.91371 5 ATCAACG 25745 0.0 32.281567 3 TATCAAC 28655 0.0 30.482378 2 TAGGCAT 9415 0.0 30.056889 5 AACGCAG 29070 0.0 29.139551 6 GTACATG 33710 0.0 28.563772 1 GGGCACC 8525 0.0 28.337269 94 CTAGGCA 10670 0.0 27.710619 4 TACCTGG 4855 0.0 27.626566 2 TAGTACT 10495 0.0 27.54563 4 CTGTGCG 10355 0.0 27.509157 9 GTGGTAT 4565 0.0 27.319565 1 TACATGG 34475 0.0 27.192951 2 TCAACGC 30955 0.0 27.090937 4 ACGCAGA 31025 0.0 26.606703 7 ACATGGG 34125 0.0 26.434286 3 >>END_MODULE