FastQCFastQC Report
Mon 6 Jun 2016
ERR1211220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8292365
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186110.2244353691618736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159910.19284004020565906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95820.11555207712154493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140050.044.0428541
GTATCAA232750.036.6010251
GTACTAG76800.033.485041
GGGCACC84600.032.17315394
CTGTGCG98700.031.229519
CAACGCA259400.031.137175
ATCAACG265300.030.4001943
TAGTACT103400.029.9998954
TACCTGG49750.029.9442022
TAGGCAT93800.028.953315
GTGGTAT49800.028.4159341
TATCAAC298300.028.4046252
AACGCAG297650.027.8935576
CTAGTAC117900.027.2670823
ACTGTGC124050.027.196078
CTAGGCA106250.027.0719074
GTACTGT120900.026.9715826
GTACATG345100.026.3200631
TCAACGC313500.026.0710414
TACTGTG136350.025.9140877
Produced by FastQC (version 0.11.5)