##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211220_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8292365 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19416595868609 33.0 31.0 34.0 30.0 34.0 2 32.33994306811145 34.0 31.0 34.0 31.0 34.0 3 32.4219528445745 34.0 31.0 34.0 31.0 34.0 4 36.020422762384435 37.0 35.0 37.0 35.0 37.0 5 35.907763225569546 37.0 35.0 37.0 35.0 37.0 6 35.93310460887817 37.0 35.0 37.0 35.0 37.0 7 35.86706759772393 37.0 35.0 37.0 35.0 37.0 8 35.85658964601775 37.0 35.0 37.0 35.0 37.0 9 37.466546033610435 39.0 37.0 39.0 35.0 39.0 10-11 37.44917173809884 39.0 37.0 39.0 34.5 39.0 12-13 37.348398677578714 39.0 37.0 39.0 34.0 39.0 14-15 38.59167227925929 40.0 38.0 41.0 34.0 41.0 16-17 38.54321764659419 40.0 38.0 41.0 34.0 41.0 18-19 38.553334060910245 40.0 38.0 41.0 34.0 41.0 20-21 38.50939490724299 40.0 38.0 41.0 34.0 41.0 22-23 38.37629180577555 40.0 38.0 41.0 34.0 41.0 24-25 38.31391008475869 40.0 38.0 41.0 34.0 41.0 26-27 38.182023825531076 40.0 38.0 41.0 33.5 41.0 28-29 38.16567257953551 40.0 38.0 41.0 33.5 41.0 30-31 38.19533384022532 40.0 38.0 41.0 34.0 41.0 32-33 38.0863390600872 40.0 37.0 41.0 33.0 41.0 34-35 37.95789844031226 40.0 37.0 41.0 33.0 41.0 36-37 37.82451357363068 40.0 37.0 41.0 33.0 41.0 38-39 37.7360324828924 40.0 37.0 41.0 33.0 41.0 40-41 37.637513061714 40.0 37.0 41.0 33.0 41.0 42-43 37.496378837641615 40.0 36.0 41.0 32.0 41.0 44-45 37.34818118835821 40.0 36.0 41.0 32.0 41.0 46-47 37.112853631020826 39.0 36.0 41.0 31.0 41.0 48-49 36.8707029297432 39.0 35.5 41.0 31.0 41.0 50-51 36.66276291504293 39.0 35.0 41.0 30.5 41.0 52-53 36.44589631546609 39.0 35.0 40.0 30.0 41.0 54-55 36.19186317775448 38.0 35.0 40.0 30.0 41.0 56-57 35.91363266088746 38.0 35.0 40.0 29.0 41.0 58-59 35.62105032762065 37.5 34.0 40.0 29.0 41.0 60-61 35.35410036823029 37.0 34.0 40.0 28.0 41.0 62-63 35.03910898760486 36.5 34.0 39.5 28.0 41.0 64-65 34.661145463326804 36.0 34.0 39.0 28.0 41.0 66-67 34.35622593795618 35.5 33.0 39.0 27.0 40.0 68-69 34.02865672217757 35.0 33.0 38.5 26.0 40.0 70-71 33.729831417213305 35.0 33.0 38.0 26.0 40.0 72-73 33.41165620423124 35.0 33.0 37.0 26.0 39.5 74-75 33.09097941299014 35.0 32.0 36.5 26.0 39.0 76-77 32.119923749135495 34.0 31.0 35.5 24.5 38.0 78-79 32.621003477295076 35.0 32.0 36.0 26.0 38.0 80-81 32.56171936474094 35.0 32.0 35.0 26.0 37.0 82-83 32.332305138522 35.0 32.0 35.0 25.0 37.0 84-85 32.10390829395474 34.0 32.0 35.0 25.0 36.5 86-87 31.883172231323634 34.0 31.5 35.0 25.0 36.0 88-89 31.681834735928774 34.0 31.0 35.0 24.5 36.0 90-91 31.481441964988278 34.0 31.0 35.0 24.0 35.0 92-93 31.310099531315856 34.0 31.0 35.0 24.0 35.0 94-95 31.13229832502549 34.0 31.0 35.0 23.0 35.0 96-97 30.971475869670474 34.0 30.5 35.0 23.0 35.0 98-99 30.80400289905232 34.0 30.0 35.0 21.0 35.0 100 28.959635399551274 32.0 27.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33142437744026765 1101 2 -0.3429599732914781 1101 3 -0.4140709906544586 1101 4 -0.23560061592930026 1101 5 -0.23598838709117587 1101 6 -0.311694117508182 1101 7 -0.24997883055483783 1101 8 -0.3124080405308902 1101 9 -0.46984502587555 1101 10-11 -0.46760367195277297 1101 12-13 -0.4675047738781828 1101 14-15 -0.711960844923702 1101 16-17 -0.6306157156648382 1101 18-19 -0.7384597240136728 1101 20-21 -0.7298366271177485 1101 22-23 -0.7058476057471026 1101 24-25 -0.7276174821078456 1101 26-27 -0.8675888817639006 1101 28-29 -0.8790612802191049 1101 30-31 -0.7140940346086708 1101 32-33 -0.665317424940163 1101 34-35 -0.7392603531640205 1101 36-37 -0.6560554239990353 1101 38-39 -0.7630908839200501 1101 40-41 -0.8443898086237098 1101 42-43 -0.7572256133976651 1101 44-45 -0.8145422732516607 1101 46-47 -0.7642652647397483 1101 48-49 -0.8130067141395472 1101 50-51 -0.8579445662070029 1101 52-53 -0.8250458493767852 1101 54-55 -0.8095530861241613 1101 56-57 -0.8989497180259676 1101 58-59 -0.8875962375258908 1101 60-61 -1.000209788990908 1101 62-63 -1.0776173212466489 1101 64-65 -0.9309403428199801 1101 66-67 -1.0010816044629962 1101 68-69 -1.0983979660507401 1101 70-71 -1.1036536686170066 1101 72-73 -1.1408709501254322 1101 74-75 -1.0880679411030982 1101 76-77 -1.027764705105234 1101 78-79 -0.9833012874464231 1101 80-81 -0.9314903573102455 1101 82-83 -0.9399329803742447 1101 84-85 -0.9247496150637744 1101 86-87 -1.021863774628848 1101 88-89 -1.073023537237269 1101 90-91 -1.0806805132274384 1101 92-93 -1.0899749949289301 1101 94-95 -1.171025391354398 1101 96-97 -1.1906790720768896 1101 98-99 -1.2918878640871547 1101 100 -1.157743536568411 1102 1 -0.4051504908876602 1102 2 -0.3529870732304268 1102 3 -0.3891287412025548 1102 4 -0.27215423912960546 1102 5 -0.3428385743739639 1102 6 -0.34933483127700526 1102 7 -0.3352994287205675 1102 8 -0.3515394761289059 1102 9 -0.5297326654766294 1102 10-11 -0.5011274859487855 1102 12-13 -0.4997143564091786 1102 14-15 -0.6537954724424395 1102 16-17 -0.6889569493111267 1102 18-19 -0.6837787654082135 1102 20-21 -0.7834657220286658 1102 22-23 -0.7487865489892371 1102 24-25 -0.7884993060542129 1102 26-27 -0.800131206335557 1102 28-29 -0.8939847666209175 1102 30-31 -0.7593702291962572 1102 32-33 -0.7282535427469057 1102 34-35 -0.7669868337325596 1102 36-37 -0.8199551984013311 1102 38-39 -0.8699059022814026 1102 40-41 -0.7830301161948086 1102 42-43 -0.8262300838908061 1102 44-45 -0.9432801550414354 1102 46-47 -0.9714503482424206 1102 48-49 -0.9315396561595719 1102 50-51 -0.9662544210288999 1102 52-53 -0.9738997167461036 1102 54-55 -0.893776336119295 1102 56-57 -0.9973042347288228 1102 58-59 -1.0171127781120788 1102 60-61 -0.9567572370982091 1102 62-63 -1.022701812096706 1102 64-65 -1.0004486859346002 1102 66-67 -1.1202891473290109 1102 68-69 -1.0791797483242505 1102 70-71 -1.1573960193765984 1102 72-73 -1.135800689442263 1102 74-75 -1.23423283479692 1102 76-77 -1.0000842719804268 1102 78-79 -0.8829255512337326 1102 80-81 -0.8533844878623142 1102 82-83 -0.8103144350138223 1102 84-85 -0.9850850788875363 1102 86-87 -0.9322229708514271 1102 88-89 -0.9959737040677688 1102 90-91 -1.0115222532134673 1102 92-93 -1.0399170854252517 1102 94-95 -1.2148676327790433 1102 96-97 -1.2870410971788893 1102 98-99 -1.3115190517144768 1102 100 -1.2837319256582802 1103 1 -0.33605035137076555 1103 2 -0.3125201927039676 1103 3 -0.3582479057505452 1103 4 -0.25217417235148787 1103 5 -0.2643053207595969 1103 6 -0.3767912916190781 1103 7 -0.43931885147659244 1103 8 -0.3947591142409763 1103 9 -0.5531615084676673 1103 10-11 -0.536336646462118 1103 12-13 -0.5654079323164396 1103 14-15 -0.6521253670035776 1103 16-17 -0.7181025009770039 1103 18-19 -0.71900707491546 1103 20-21 -0.7533625249361293 1103 22-23 -0.7563928751643871 1103 24-25 -0.8879464579626912 1103 26-27 -0.8169051051933778 1103 28-29 -0.886838799222879 1103 30-31 -0.8333146400909968 1103 32-33 -0.752534760630752 1103 34-35 -0.8211967221033873 1103 36-37 -0.8715357374386627 1103 38-39 -0.8459558282429711 1103 40-41 -0.8760525686022191 1103 42-43 -0.8243078119732701 1103 44-45 -0.8651372864529918 1103 46-47 -0.897705778466495 1103 48-49 -0.866741418049692 1103 50-51 -0.927856055928892 1103 52-53 -0.9999833866928682 1103 54-55 -0.9750174271069696 1103 56-57 -0.9486197608244353 1103 58-59 -0.9370188978043146 1103 60-61 -0.9963305740260253 1103 62-63 -0.936948441743283 1103 64-65 -0.937312397814722 1103 66-67 -1.048383141943546 1103 68-69 -1.1425725416215897 1103 70-71 -1.0693210863295732 1103 72-73 -0.9445688865321884 1103 74-75 -0.9823472623992373 1103 76-77 -0.9706760822395388 1103 78-79 -0.8316398153617115 1103 80-81 -0.7828587106415128 1103 82-83 -0.8162106614289186 1103 84-85 -0.9233395231504602 1103 86-87 -0.8977167774078332 1103 88-89 -0.9540384186635968 1103 90-91 -0.9497011453981763 1103 92-93 -1.0091853315783617 1103 94-95 -0.9982117073822216 1103 96-97 -1.0341583417384896 1103 98-99 -0.9977013269098549 1103 100 -1.1100821939660257 1104 1 -0.3549312092590071 1104 2 -0.3576841014855958 1104 3 -0.41279835447654634 1104 4 -0.27010535209562647 1104 5 -0.29243722624999435 1104 6 -0.4042802243582173 1104 7 -0.33437264193645433 1104 8 -0.39841555985804433 1104 9 -0.4963181597867461 1104 10-11 -0.5342660771232914 1104 12-13 -0.5757632425076835 1104 14-15 -0.736794223719194 1104 16-17 -0.6667586017044727 1104 18-19 -0.7205704717823878 1104 20-21 -0.7802007864954774 1104 22-23 -0.7742036514862392 1104 24-25 -0.8202338520015218 1104 26-27 -0.8720624980250946 1104 28-29 -0.9279915188005319 1104 30-31 -0.8832528063130027 1104 32-33 -0.8208709393032692 1104 34-35 -0.8924512210650235 1104 36-37 -0.8935504716150007 1104 38-39 -0.8196881742066537 1104 40-41 -0.8383903165224069 1104 42-43 -0.8144689123681701 1104 44-45 -0.8815580345185268 1104 46-47 -0.9560857334830359 1104 48-49 -0.8986350836595207 1104 50-51 -0.9286231273481391 1104 52-53 -0.9154798922221872 1104 54-55 -0.9301035632841561 1104 56-57 -0.8903540504873533 1104 58-59 -0.9101319821766296 1104 60-61 -0.9523605375416366 1104 62-63 -1.0090645488265793 1104 64-65 -1.0196597164212733 1104 66-67 -0.9811382925001695 1104 68-69 -1.0650542932665985 1104 70-71 -1.0912412838035053 1104 72-73 -1.0554493527243878 1104 74-75 -1.0626144656155816 1104 76-77 -0.9594640909209033 1104 78-79 -0.8203674683633579 1104 80-81 -0.8462059996860951 1104 82-83 -0.8360113275585412 1104 84-85 -0.8669824072158221 1104 86-87 -0.9206756468597312 1104 88-89 -0.9670074993111051 1104 90-91 -0.8961942956444027 1104 92-93 -1.0082941614911292 1104 94-95 -0.9630397429649697 1104 96-97 -1.0045123093237898 1104 98-99 -1.2293026985850588 1104 100 -1.272750604788662 1105 1 -0.42111842538947286 1105 2 -0.38554870066398195 1105 3 -0.3973529192675507 1105 4 -0.24844215136694459 1105 5 -0.31250200542564954 1105 6 -0.38903343813365154 1105 7 -0.4083415055599815 1105 8 -0.49805593652203584 1105 9 -0.6263960896500436 1105 10-11 -0.6106330286545969 1105 12-13 -0.6443741432935113 1105 14-15 -0.8533986550240868 1105 16-17 -0.7958174283623904 1105 18-19 -0.7424880707730424 1105 20-21 -0.8430192209187553 1105 22-23 -0.8151944720572217 1105 24-25 -0.8986701242498114 1105 26-27 -0.9085072721057017 1105 28-29 -0.9039778043432776 1105 30-31 -0.8621212316398967 1105 32-33 -0.9320943926328766 1105 34-35 -0.8835574480399728 1105 36-37 -0.9027671821167331 1105 38-39 -0.8330535488635675 1105 40-41 -0.8696983531823506 1105 42-43 -0.8942391092565956 1105 44-45 -0.8900747659329866 1105 46-47 -0.856457952713626 1105 48-49 -0.9130485160497344 1105 50-51 -0.8834411641697315 1105 52-53 -0.9811329259969668 1105 54-55 -1.0055581772134943 1105 56-57 -0.9617778853485461 1105 58-59 -1.0821581893315724 1105 60-61 -1.0127876164910887 1105 62-63 -1.0606299223968492 1105 64-65 -1.0081509411913885 1105 66-67 -1.0306590984376456 1105 68-69 -1.052406774924421 1105 70-71 -1.0715075581464433 1105 72-73 -1.009945616324785 1105 74-75 -1.0583367631431955 1105 76-77 -1.0275086320289262 1105 78-79 -0.987378779966015 1105 80-81 -0.9466668390076691 1105 82-83 -0.915687270371798 1105 84-85 -0.8679678229415941 1105 86-87 -0.8901208835041068 1105 88-89 -0.9162851057745911 1105 90-91 -0.9724993054087925 1105 92-93 -1.0411755797743467 1105 94-95 -1.0496164980607254 1105 96-97 -1.0662926665517993 1105 98-99 -1.1179415593883917 1105 100 -1.0841623739133546 1106 1 -0.4357787870549821 1106 2 -0.42180981199789613 1106 3 -0.48628550557927497 1106 4 -0.30988013398475545 1106 5 -0.36595742522426633 1106 6 -0.38808957916551634 1106 7 -0.44262640798787345 1106 8 -0.37227117016703204 1106 9 -0.47179753686469184 1106 10-11 -0.5634490500603562 1106 12-13 -0.6402805389607451 1106 14-15 -0.7703480021089391 1106 16-17 -0.6990359378163333 1106 18-19 -0.6850588229751153 1106 20-21 -0.7104431834015301 1106 22-23 -0.7095521986299858 1106 24-25 -0.7645684474213823 1106 26-27 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1107 48-49 -0.8150468477474391 1107 50-51 -0.8564717405790603 1107 52-53 -0.799353120773084 1107 54-55 -0.7728794726267907 1107 56-57 -0.8521649618484872 1107 58-59 -0.9165536212069654 1107 60-61 -0.7823264104610317 1107 62-63 -0.7665620013015442 1107 64-65 -0.7964103050909017 1107 66-67 -0.8352935722176653 1107 68-69 -0.7515777732683375 1107 70-71 -0.804148268749465 1107 72-73 -0.8027852193256138 1107 74-75 -0.7994441738845666 1107 76-77 -0.7996259169148132 1107 78-79 -0.6863236794470069 1107 80-81 -0.6403765570500539 1107 82-83 -0.6903431706460061 1107 84-85 -0.699016615336987 1107 86-87 -0.7377359605467326 1107 88-89 -0.6700835138563832 1107 90-91 -0.7284810801800639 1107 92-93 -0.769351320599263 1107 94-95 -0.7895835281294445 1107 96-97 -0.8347670017973776 1107 98-99 -0.8891636089707049 1107 100 -0.8064580753776909 1108 1 -0.4392082316229846 1108 2 -0.3708922413024318 1108 3 -0.44138485401957084 1108 4 -0.23745267457338315 1108 5 -0.28068947850224646 1108 6 -0.3356692531385548 1108 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0.5677442819749814 2116 74-75 0.7037594317370903 2116 76-77 0.6485447158902176 2116 78-79 0.5920864767982934 2116 80-81 0.5234114383762645 2116 82-83 0.5344596724094828 2116 84-85 0.5209492986452631 2116 86-87 0.5498414303318135 2116 88-89 0.5825934506895329 2116 90-91 0.478919942199898 2116 92-93 0.5283887273492844 2116 94-95 0.25604286178405644 2116 96-97 0.5254968529433164 2116 98-99 0.26975372036820744 2116 100 -0.23176938056689167 2201 1 0.6485512576044243 2201 2 0.6164617439819224 2201 3 0.5846825376550058 2201 4 0.381290357739168 2201 5 0.4627917272491615 2201 6 0.489901421230023 2201 7 0.5299701759530748 2201 8 0.5281816564498527 2201 9 0.6448639634928384 2201 10-11 0.6416588720164 2201 12-13 0.6824936434850386 2201 14-15 0.8180100450443817 2201 16-17 -0.6479138222900076 2201 18-19 -0.11082556536760535 2201 20-21 0.5534374184977295 2201 22-23 0.31150161423682476 2201 24-25 0.5653566179917036 2201 26-27 0.7837190010509332 2201 28-29 0.7992055590857987 2201 30-31 0.9557432164898216 2201 32-33 0.9699316181880064 2201 34-35 1.036803258803026 2201 36-37 1.0467561777005159 2201 38-39 1.0264204366652407 2201 40-41 1.0082498042392416 2201 42-43 1.1399410530470249 2201 44-45 1.0412423803516475 2201 46-47 1.0801319276144383 2201 48-49 1.1281650049545249 2201 50-51 1.1159711356156166 2201 52-53 1.1459304128001335 2201 54-55 1.1778350485886264 2201 56-57 1.1491547393451214 2201 58-59 1.1939703470354175 2201 60-61 1.0865946432081799 2201 62-63 1.0334266171425597 2201 64-65 -1.075147237624563 2201 66-67 0.4034857237534055 2201 68-69 0.9523955782307425 2201 70-71 1.0885499877471787 2201 72-73 1.096648485348453 2201 74-75 1.1019435649638112 2201 76-77 1.2297492915955743 2201 78-79 1.010397048211935 2201 80-81 0.9192082230971508 2201 82-83 0.9481019214565123 2201 84-85 0.9801576541810562 2201 86-87 0.9504780608030536 2201 88-89 0.9823506849505996 2201 90-91 0.9936462165102533 2201 92-93 0.952688712835112 2201 94-95 1.0204575489404668 2201 96-97 1.050884142048993 2201 98-99 1.0738842123002783 2201 100 1.3293580870468986 2202 1 0.6646192306443055 2202 2 0.5967082259671344 2202 3 0.5961223146340302 2202 4 0.4097125763079177 2202 5 0.466905984772751 2202 6 0.5033564518638869 2202 7 0.5071876779839926 2202 8 0.5146590020110438 2202 9 0.6823828096712532 2202 10-11 0.6809852982163704 2202 12-13 0.6244316446191434 2202 14-15 0.8668947604530217 2202 16-17 0.8951873320919006 2202 18-19 0.8269849081720722 2202 20-21 0.8469427387726114 2202 22-23 -0.6590692782543428 2202 24-25 0.5450102967540644 2202 26-27 0.8296092862950886 2202 28-29 0.9371877021167805 2202 30-31 1.0156689700269297 2202 32-33 1.0168535711054218 2202 34-35 0.9675862955726942 2202 36-37 -0.944973390490432 2202 38-39 0.7014777830085848 2202 40-41 1.0000169332764415 2202 42-43 0.5862479423465174 2202 44-45 0.987106418228791 2202 46-47 1.0614401699851044 2202 48-49 1.0940361679671042 2202 50-51 1.1209877520055755 2202 52-53 1.0688136811878763 2202 54-55 0.9933025086730325 2202 56-57 1.0255881658514099 2202 58-59 1.149640973213522 2202 60-61 0.21994080665693616 2202 62-63 0.9308982354363948 2202 64-65 1.1719744185526366 2202 66-67 1.1800636345529583 2202 68-69 0.8377425232212232 2202 70-71 1.0945842757536113 2202 72-73 1.156691461811775 2202 74-75 1.1960746696456752 2202 76-77 1.266943794629988 2202 78-79 0.9967705599318393 2202 80-81 0.9877624682785111 2202 82-83 1.0291178402334822 2202 84-85 1.0415826043849634 2202 86-87 1.0620810763153763 2202 88-89 0.9884347089445065 2202 90-91 0.4425661434270367 2202 92-93 0.9040978313089454 2202 94-95 1.115108358392174 2202 96-97 1.1319790504763354 2202 98-99 1.18189739396745 2202 100 1.4151563429811276 2203 1 0.40733549037913974 2203 2 0.4905196026086003 2203 3 0.5306419721540792 2203 4 0.37443515266190985 2203 5 -0.8630146552287243 2203 6 0.009240765246183003 2203 7 0.27938558043231154 2203 8 0.4098546580156892 2203 9 0.5806305386812056 2203 10-11 0.6173991848476703 2203 12-13 0.6914987851061412 2203 14-15 0.8689141657114448 2203 16-17 0.8986670764738847 2203 18-19 0.8761674179621011 2203 20-21 0.8493512210053282 2203 22-23 0.012229969878937652 2203 24-25 0.7048938006529042 2203 26-27 0.8647092194332018 2203 28-29 0.8741755038856738 2203 30-31 0.9821759389795588 2203 32-33 0.35210292824468326 2203 34-35 -0.34759601760991643 2203 36-37 0.0511362535526132 2203 38-39 0.8206129397308857 2203 40-41 1.0240764910185192 2203 42-43 0.3485095920600827 2203 44-45 0.9421922282628685 2203 46-47 1.0705051669428869 2203 48-49 0.8722842646553914 2203 50-51 0.9671268960279562 2203 52-53 1.0530100034469356 2203 54-55 0.9374972313540155 2203 56-57 0.8091547000533907 2203 58-59 0.9160033239927188 2203 60-61 1.0690336490415717 2203 62-63 1.0010458523753911 2203 64-65 0.9685966014612575 2203 66-67 1.1073358969882747 2203 68-69 1.1193696015126449 2203 70-71 1.1748871682175661 2203 72-73 1.173535515539868 2203 74-75 1.0908273796625636 2203 76-77 0.7994816482078235 2203 78-79 0.8503454248263083 2203 80-81 0.9260806212848323 2203 82-83 0.9788848580802494 2203 84-85 1.0069195818716423 2203 86-87 1.0086230468265107 2203 88-89 1.0206047245873826 2203 90-91 1.085016072820384 2203 92-93 1.1247592550717584 2203 94-95 1.154435481035538 2203 96-97 1.125292500805866 2203 98-99 0.9711886147724158 2203 100 1.3712265733256892 2204 1 0.6665078708822776 2204 2 0.6200307841326449 2204 3 0.5746318795815029 2204 4 0.4012805440152576 2204 5 0.44996772172724775 2204 6 0.487871098745174 2204 7 0.5085890290438115 2204 8 0.4723710952397653 2204 9 0.6757771938172468 2204 10-11 0.6396344028111045 2204 12-13 0.7013682676974256 2204 14-15 0.8356410534535144 2204 16-17 0.8502987722151332 2204 18-19 0.8424857090710773 2204 20-21 0.8287854283544149 2204 22-23 0.8481954350956684 2204 24-25 0.8145483943247243 2204 26-27 0.8538359196622238 2204 28-29 0.8844775253015627 2204 30-31 0.9584518009920586 2204 32-33 0.9410298196836564 2204 34-35 1.0037398256465835 2204 36-37 1.0238537080829246 2204 38-39 0.9960014139259599 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2205 4 0.3739648190799727 2205 5 0.4664036825101405 2205 6 0.492744214564091 2205 7 0.5007004588386934 2205 8 0.5101929573477832 2205 9 0.6930086397950177 2205 10-11 0.667953280676592 2205 12-13 0.7014730371097144 2205 14-15 0.8769409344916212 2205 16-17 0.887037696525347 2205 18-19 0.8507314545732712 2205 20-21 0.8509127039001712 2205 22-23 0.8566736822638887 2205 24-25 0.8991546592181336 2205 26-27 0.8821678719810748 2205 28-29 0.9317260972491752 2205 30-31 0.96182632493597 2205 32-33 0.9877169489994415 2205 34-35 1.0218597957184272 2205 36-37 1.0667761189356497 2205 38-39 1.0311894717191024 2205 40-41 1.048439752993616 2205 42-43 1.0641250359385026 2205 44-45 1.0448553142801558 2205 46-47 1.0368756130570134 2205 48-49 1.0844980037722252 2205 50-51 1.066048308855919 2205 52-53 1.0702965634496096 2205 54-55 0.9975065782895953 2205 56-57 1.054454394960267 2205 58-59 0.9953502859311882 2205 60-61 1.098504431522393 2205 62-63 1.1121682664850425 2205 64-65 0.5487939237047641 2205 66-67 0.9627753479085186 2205 68-69 1.0741656599872158 2205 70-71 1.0801492118406983 2205 72-73 1.018517730886586 2205 74-75 1.0167908651207895 2205 76-77 1.0248339811203735 2205 78-79 0.9279930717089186 2205 80-81 0.9810891819885228 2205 82-83 0.9707100668709359 2205 84-85 0.887852887445284 2205 86-87 0.941106546937629 2205 88-89 1.0134190043155122 2205 90-91 1.0502093672725685 2205 92-93 0.9884426328996625 2205 94-95 1.0877871744209138 2205 96-97 1.1388321167908764 2205 98-99 1.1323600036620363 2205 100 1.4395059377692085 2206 1 0.4757974878851101 2206 2 0.5072546581617701 2206 3 0.5416579242854667 2206 4 0.36929178543775976 2206 5 -0.5108394310489075 2206 6 0.16369130686104683 2206 7 0.3774249843351285 2206 8 0.4554996595716645 2206 9 0.6519558724673118 2206 10-11 0.6817631303574387 2206 12-13 0.7100641099537839 2206 14-15 0.9028687532888711 2206 16-17 0.9044951754899131 2206 18-19 0.9038007255431566 2206 20-21 0.9078026371997368 2206 22-23 0.9697551815610765 2206 24-25 0.9209307752063225 2206 26-27 0.9761013405449219 2206 28-29 0.8950806214662137 2206 30-31 0.9482596959696181 2206 32-33 0.9725251347896133 2206 34-35 1.0214402710763366 2206 36-37 1.100402485785132 2206 38-39 1.0906171492040357 2206 40-41 1.0016870825828192 2206 42-43 1.0892842486577337 2206 44-45 1.0889733747086723 2206 46-47 1.0849519343100624 2206 48-49 1.0832928712819268 2206 50-51 1.099137126750783 2206 52-53 1.1039662694808285 2206 54-55 1.1334687294189862 2206 56-57 1.1454875625518994 2206 58-59 1.0917132188694225 2206 60-61 1.1036256440927303 2206 62-63 1.126192869988607 2206 64-65 1.2086390815307766 2206 66-67 1.2422211617507202 2206 68-69 1.2216052325783835 2206 70-71 0.9115892498510689 2206 72-73 1.044959282531508 2206 74-75 1.180749037358943 2206 76-77 1.169382366914725 2206 78-79 1.0803324420335372 2206 80-81 1.0144086140748811 2206 82-83 1.0307893823435563 2206 84-85 1.057356192143068 2206 86-87 1.0615334923924458 2206 88-89 1.096361118058038 2206 90-91 1.1826563938963552 2206 92-93 1.1611676197266974 2206 94-95 1.2386435917458805 2206 96-97 1.2914518459652875 2206 98-99 1.2904255974454557 2206 100 1.4966000285978431 2207 1 0.6922105244089636 2207 2 0.6297811606154724 2207 3 0.5959893015072382 2207 4 0.3892011786884879 2207 5 0.4919642515538172 2207 6 0.48417571287717465 2207 7 0.538324539186398 2207 8 0.5079224672404266 2207 9 0.6715520136658526 2207 10-11 0.6413378762873378 2207 12-13 0.7033573272436868 2207 14-15 0.8792716143220076 2207 16-17 0.9019464087787625 2207 18-19 0.8747707353339464 2207 20-21 0.9062290273669831 2207 22-23 0.9516482409596705 2207 24-25 0.925160172429571 2207 26-27 0.9729825301292223 2207 28-29 0.8974098218505375 2207 30-31 0.9652449827748129 2207 32-33 1.0386551667566692 2207 34-35 1.0635949595438419 2207 36-37 1.0929047459063668 2207 38-39 1.0972443314470937 2207 40-41 1.117105986350012 2207 42-43 1.1478671229753488 2207 44-45 1.084721775298604 2207 46-47 1.1450119190938963 2207 48-49 1.1487788863597714 2207 50-51 1.109730042459276 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0.8765616019945242 2311 32-33 0.9813919518870122 2311 34-35 1.0091575331951432 2311 36-37 0.9691048252941385 2311 38-39 0.9813178828733982 2311 40-41 0.9892810491907227 2311 42-43 0.9927771358647348 2311 44-45 1.096820566578458 2311 46-47 0.7158813405126025 2311 48-49 0.9539816187266723 2311 50-51 1.0420857322318042 2311 52-53 0.9587740743309467 2311 54-55 0.9651251483121897 2311 56-57 1.0564006320992405 2311 58-59 1.0993845503013873 2311 60-61 1.171852243253774 2311 62-63 1.098551529878769 2311 64-65 1.1255805676065478 2311 66-67 1.1436047785554635 2311 68-69 1.1966407674794226 2311 70-71 1.1958857744000042 2311 72-73 1.221276065874207 2311 74-75 1.183232502530423 2311 76-77 1.197677237633826 2311 78-79 0.9619168417581179 2311 80-81 0.9072908921451059 2311 82-83 1.0074767095581478 2311 84-85 1.101456633525224 2311 86-87 1.1003268692693915 2311 88-89 1.1518446920124852 2311 90-91 1.1938171692772208 2311 92-93 1.2220515570453685 2311 94-95 1.2650335446007865 2311 96-97 1.2289802513178465 2311 98-99 1.265324606774243 2311 100 1.3646772740793054 2312 1 0.2832024670396862 2312 2 0.3534492160154059 2312 3 0.43953203136975816 2312 4 0.15413637679519354 2312 5 0.33846012779697077 2312 6 0.41748660237951185 2312 7 0.3010127057529033 2312 8 0.40212245162142324 2312 9 0.556334383204721 2312 10-11 0.6085397824850318 2312 12-13 0.6462046548658904 2312 14-15 0.8427659252692337 2312 16-17 0.8346157476534444 2312 18-19 0.7868795047261372 2312 20-21 0.8262184845386571 2312 22-23 0.932413996630892 2312 24-25 0.9302072841020603 2312 26-27 0.9227193498960915 2312 28-29 0.9748379659392512 2312 30-31 0.9569998119334571 2312 32-33 0.8194148445977163 2312 34-35 0.9343591702917635 2312 36-37 1.0302395834177815 2312 38-39 0.8743394172327825 2312 40-41 0.9859432193414222 2312 42-43 1.0683305320608554 2312 44-45 0.8383250118280614 2312 46-47 0.8794201455558834 2312 48-49 1.0709876852922449 2312 50-51 0.9146802993239973 2312 52-53 1.151559882917546 2312 54-55 0.976274296945121 2312 56-57 1.0355478527853137 2312 58-59 1.139488956804115 2312 60-61 1.1648166190740668 2312 62-63 1.1921215396926144 2312 64-65 1.2003010111669852 2312 66-67 1.1962180779065221 2312 68-69 1.2502915763962932 2312 70-71 1.104543820026791 2312 72-73 1.1256880462288308 2312 74-75 1.1989386863038831 2312 76-77 1.202258919319739 2312 78-79 1.0975210123803052 2312 80-81 1.0849712371193547 2312 82-83 1.1101383208916928 2312 84-85 1.1186124310322185 2312 86-87 1.1313247890434788 2312 88-89 1.1265745044660385 2312 90-91 1.208901186691751 2312 92-93 1.2400279092958542 2312 94-95 1.2824101163431543 2312 96-97 0.8977113522014797 2312 98-99 0.6821681354807794 2312 100 0.9670870418274866 2313 1 0.027131979642696535 2313 2 0.18966581342247935 2313 3 0.2606815946720218 2313 4 0.17772193502169387 2313 5 0.29368312405278374 2313 6 0.22910424057820933 2313 7 0.195169223679315 2313 8 0.34675528818072365 2313 9 0.5305062526351847 2313 10-11 0.32901026467991557 2313 12-13 0.41984002320246105 2313 14-15 0.46786003418263533 2313 16-17 0.7158362293838536 2313 18-19 0.651211511258488 2313 20-21 0.6137863036032414 2313 22-23 0.5674540116639051 2313 24-25 0.46156190304954237 2313 26-27 0.6714719419885284 2313 28-29 0.5451117158493446 2313 30-31 0.7871122436525724 2313 32-33 0.8455695980566489 2313 34-35 0.8514204826170939 2313 36-37 0.885963961282414 2313 38-39 0.9297880625501307 2313 40-41 0.9570129118256432 2313 42-43 1.011691624969039 2313 44-45 0.8712788307137558 2313 46-47 0.8660380451866629 2313 48-49 0.9115363741639158 2313 50-51 0.8897432832221668 2313 52-53 0.872110870268223 2313 54-55 0.8653920074689481 2313 56-57 0.616394754708999 2313 58-59 0.6776455076417776 2313 60-61 0.5409289462203546 2313 62-63 0.8555098480784196 2313 64-65 1.0419493762609946 2313 66-67 0.9832228863988988 2313 68-69 1.0460022164696028 2313 70-71 1.035662332629542 2313 72-73 1.0535986040771093 2313 74-75 1.111225181890461 2313 76-77 1.0254118167436062 2313 78-79 0.8867137021554541 2313 80-81 0.7984501602527487 2313 82-83 0.8589203207754394 2313 84-85 0.8398250830708989 2313 86-87 0.8619885743835844 2313 88-89 0.8859149206181378 2313 90-91 0.8380636777440067 2313 92-93 0.9925689574500467 2313 94-95 1.1001195838206108 2313 96-97 1.166364446163879 2313 98-99 1.1916148841548377 2313 100 1.2232415909181924 2314 1 -0.42858299886649576 2314 2 -0.006869792508858552 2314 3 0.2324722237308663 2314 4 0.17760322604772938 2314 5 0.29240376732767004 2314 6 0.3640962324924004 2314 7 0.40556951515090844 2314 8 0.43149135690284623 2314 9 0.42708925090531835 2314 10-11 0.5545469976413813 2314 12-13 0.44179363964107665 2314 14-15 0.7627278578373407 2314 16-17 0.7760470012734046 2314 18-19 0.8040166153047039 2314 20-21 0.8188014663726548 2314 22-23 0.8583652894809788 2314 24-25 0.7543971777156386 2314 26-27 0.7397721113997591 2314 28-29 0.782017762424907 2314 30-31 0.8022777816991322 2314 32-33 0.8456280555899056 2314 34-35 0.8299365173707045 2314 36-37 0.7996128003526195 2314 38-39 0.570206899291783 2314 40-41 0.62973695759338 2314 42-43 0.7863167584544186 2314 44-45 0.82486393297809 2314 46-47 0.7613218915332922 2314 48-49 0.8023867441568129 2314 50-51 0.7308002418783488 2314 52-53 0.7969231383719872 2314 54-55 0.7100049106532751 2314 56-57 0.6583035591321149 2314 58-59 0.7951981941851258 2314 60-61 0.88099743076301 2314 62-63 0.7976548551549243 2314 64-65 0.8081424499781065 2314 66-67 0.5444003203538585 2314 68-69 0.5414257511748986 2314 70-71 0.6752995597471809 2314 72-73 0.8793577369740078 2314 74-75 0.9307599588499045 2314 76-77 0.7594679769139603 2314 78-79 0.6817365121553038 2314 80-81 0.7359939709982868 2314 82-83 0.7475711269221961 2314 84-85 0.7904738595513621 2314 86-87 0.8622312859138965 2314 88-89 0.8119652285439081 2314 90-91 0.9608729849257536 2314 92-93 0.8668123847597968 2314 94-95 0.9357366995973599 2314 96-97 0.9040899455151319 2314 98-99 1.0595731337690353 2314 100 1.0754985878001548 2315 1 0.3221955766077045 2315 2 0.38497521416584135 2315 3 0.4175320955165276 2315 4 0.2754824432619358 2315 5 0.35262891932531915 2315 6 0.3914040989449816 2315 7 0.31776858988245493 2315 8 0.23099710349156766 2315 9 0.45211635903208247 2315 10-11 0.5158566835546736 2315 12-13 0.3838322668420986 2315 14-15 0.6045730733573933 2315 16-17 0.7640984574267407 2315 18-19 0.7183850293665088 2315 20-21 0.47424257699552896 2315 22-23 0.8156639124382608 2315 24-25 0.8455450168345493 2315 26-27 0.7946563150056036 2315 28-29 0.807455982628106 2315 30-31 0.8935174710040812 2315 32-33 0.8882594276704552 2315 34-35 0.8945071355028276 2315 36-37 0.9065872535537167 2315 38-39 0.8810841291336047 2315 40-41 0.9026299586008548 2315 42-43 0.9697653883453654 2315 44-45 1.013845001564576 2315 46-47 0.9790319774272049 2315 48-49 0.8599713599398342 2315 50-51 1.0022658684394656 2315 52-53 1.0196665643379745 2315 54-55 0.8810808944401956 2315 56-57 0.8615390539869949 2315 58-59 0.8924661419370281 2315 60-61 0.9998467680549368 2315 62-63 1.0648071777658572 2315 64-65 1.1644300316992897 2315 66-67 1.1308220517987877 2315 68-69 1.1123130014167373 2315 70-71 1.0331371081366498 2315 72-73 1.1295492272777352 2315 74-75 0.565410827897189 2315 76-77 0.552070194183294 2315 78-79 0.8707250365208736 2315 80-81 0.874325760268313 2315 82-83 0.8572262981837326 2315 84-85 0.9407717983312622 2315 86-87 0.9635430644576211 2315 88-89 0.9638841622964911 2315 90-91 1.0385404413063064 2315 92-93 1.0976010767028797 2315 94-95 1.0465083998825655 2315 96-97 1.1366973254415846 2315 98-99 1.1749255644783254 2315 100 1.118877271205637 2316 1 0.22836457426561196 2316 2 0.07333959956098823 2316 3 0.18266454876667382 2316 4 -0.035631093593913477 2316 5 0.25467379454524064 2316 6 0.352857920651779 2316 7 0.4228985226573698 2316 8 0.43755656787609354 2316 9 0.6115283267782701 2316 10-11 0.5940767148350616 2316 12-13 0.35940026359403277 2316 14-15 0.49637806265675266 2316 16-17 0.7544616541839773 2316 18-19 0.6868982097137533 2316 20-21 0.7017264660267415 2316 22-23 0.7524424781347179 2316 24-25 0.45270613604144216 2316 26-27 0.6935262191654559 2316 28-29 0.9257031369536151 2316 30-31 0.577754785129379 2316 32-33 0.6751097586225043 2316 34-35 0.8289630667108199 2316 36-37 0.8147878929018475 2316 38-39 0.7433667898780527 2316 40-41 0.7541733978948528 2316 42-43 0.7653440650420578 2316 44-45 0.5899306166039935 2316 46-47 0.7402973118702647 2316 48-49 0.8345646901581034 2316 50-51 0.8438620907892513 2316 52-53 0.8788225010504576 2316 54-55 0.7548800473287471 2316 56-57 0.8984157588774266 2316 58-59 0.806030240850582 2316 60-61 0.9260146503301172 2316 62-63 0.9022145792717708 2316 64-65 1.0014032720330164 2316 66-67 1.0076618021351607 2316 68-69 0.9866905702631641 2316 70-71 0.9604876271931388 2316 72-73 1.0264961102279386 2316 74-75 0.9565145849780023 2316 76-77 0.799652659042529 2316 78-79 0.568917288770372 2316 80-81 0.5736146054892046 2316 82-83 0.5036588427752449 2316 84-85 0.6242419816058273 2316 86-87 0.6641805398271181 2316 88-89 0.666167437639583 2316 90-91 0.5570925151938972 2316 92-93 0.3503538785070255 2316 94-95 0.43480735225054445 2316 96-97 0.6278375135294603 2316 98-99 0.8493030402037469 2316 100 0.3450159031380302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 3.0 12 27.0 13 165.0 14 596.0 15 1714.0 16 4013.0 17 7949.0 18 13684.0 19 21003.0 20 30924.0 21 45072.0 22 56382.0 23 59307.0 24 60801.0 25 67505.0 26 80606.0 27 98857.0 28 123097.0 29 152436.0 30 189108.0 31 235534.0 32 301568.0 33 399140.0 34 574969.0 35 814834.0 36 1298796.0 37 1902388.0 38 1505623.0 39 246241.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20056752100455 20.696878151889084 13.510016070110078 23.59253825699629 2 15.470664882696312 19.56357444468496 40.89142241085625 24.074338261762477 3 19.11508960350877 25.356553890235173 28.913645262841182 26.614711243414874 4 12.628664943855847 16.039306819160597 38.40358600716034 32.92844222982322 5 13.34364804250657 38.04737249264836 34.47765504774573 14.131324417099345 6 31.4703706361213 37.25451062513529 17.080290122299246 14.194828616444163 7 28.150308472660264 31.679471104965117 22.034109446261457 18.136110976113162 8 26.639577490860567 35.93980728055266 18.97904879970913 18.44156642887765 9 26.11936689284184 15.547978190794009 20.99623933875271 37.33641557761144 10-11 26.2907130500637 26.090558328866358 26.627855724593857 20.990872896476084 12-13 25.97755911071428 23.406838316171154 27.699070819741006 22.916531753373555 14-15 23.282711389801626 24.840345366408386 25.13357940343226 26.743363840357727 16-17 22.23565832354116 27.746698879543715 26.311072054967383 23.706570741947747 18-19 21.77202764229505 27.53265805352273 28.55948212602798 22.135832178154242 20-21 23.104493857782156 26.63990348710012 28.210143477799992 22.045459177317735 22-23 23.329556766977817 26.440472651650044 27.888539638571142 22.341430942801 24-25 22.77538238978602 26.34131276105468 28.604230852533018 22.279073996626277 26-27 22.811355625538564 26.597094749178176 27.698100069696935 22.893449555586333 28-29 22.175708805362245 26.87923647221617 28.310187805013793 22.6348669174078 30-31 22.893700591552207 26.63099243955214 28.222096333678742 22.25321063521691 32-33 23.064219474204076 26.792232134516663 27.580016008719703 22.563532382559554 34-35 23.074469301614627 26.694547350319798 28.107648203760387 22.123335144305184 36-37 22.807587681625026 26.359118039161118 27.79337831523484 23.039915963979013 38-39 22.875047946213396 26.530177768878747 28.281722300356794 22.313051984551066 40-41 22.57314029040855 25.77641481822362 28.817670574050535 22.83277431731729 42-43 22.653434484290567 25.959864132743416 28.215838714643127 23.17086266832289 44-45 22.762015129384565 26.574868973364225 28.014122190135897 22.648993707115313 46-47 22.768074196842747 26.573449810880984 27.45556496222143 23.20291103005484 48-49 23.379253572604824 26.506950806789963 27.504230252100232 22.609565368504974 50-51 22.961646425693445 26.339245951659624 27.56046436837532 23.138643254271614 52-53 22.600544310546105 26.769963576143564 27.288301448011264 23.341190665299067 54-55 22.563166326006655 26.707113698633027 27.259570867885525 23.470149107474793 56-57 23.301002251478174 26.353841441428404 26.995548898801662 23.34960740829176 58-59 22.401930228571228 26.76737066116009 27.575538096380093 23.255161013888593 60-61 22.562582033366834 27.230274953142956 27.063686203162778 23.14345681032743 62-63 22.86423364671043 26.679762033109007 26.733408536119164 23.722595784061397 64-65 22.279319104747046 26.784983138548608 27.02765075358395 23.9080470031204 66-67 22.6054425335349 26.58143664066161 27.069979439017434 23.743141386786057 68-69 22.34623931940551 26.52511184827272 27.287189633637706 23.841459198684063 70-71 22.24565173076219 26.219441971486063 27.360262568153658 24.174643729598095 72-73 22.65606176849675 26.282249966429376 27.447967948833185 23.613720316240688 74-75 22.824806576924416 26.58919549909876 27.083145216729328 23.5028527072475 76-77 22.622763208258327 26.49986688330449 26.728312503716957 24.14905740472023 78-79 22.53409764418031 26.491640013402417 26.784763603899243 24.18949873851803 80-81 22.12436113562864 26.920783772516348 27.172784043130378 23.782071048724635 82-83 22.87705690474994 26.07898169209326 27.96669032618467 23.07727107697213 84-85 22.972113865967504 26.374151030587633 27.008695715125143 23.645039388319724 86-87 21.934133983635455 26.91073107103167 27.366378961873583 23.788755983459286 88-89 22.669838425654177 25.978089086008215 27.272175441780718 24.07989704655689 90-91 22.439092867827487 26.098708850600254 27.2527307856859 24.209467495886358 92-93 22.67442824894561 26.18092354939175 27.185823820310006 23.958824381352635 94-95 21.81381324227819 26.097744492846935 27.78427157965947 24.3041706852154 96-97 22.076373106545965 26.009734619464055 27.42197199608541 24.491920277904573 98-99 21.532563611456787 26.404515659860948 27.35023961685427 24.712681111827994 100 22.095310635292854 25.899586147728627 26.77626729063981 25.22883592633871 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 545.0 1 377.0 2 310.0 3 437.0 4 390.0 5 380.5 6 614.5 7 1220.5 8 2079.0 9 2613.5 10 2537.0 11 2416.0 12 2785.5 13 3368.0 14 4045.5 15 4967.0 16 5834.5 17 6678.5 18 7628.5 19 8414.0 20 9225.0 21 10294.5 22 11649.5 23 13356.5 24 15551.5 25 18317.0 26 22654.5 27 27709.5 28 33790.0 29 45379.5 30 61926.0 31 79036.0 32 101915.0 33 128153.0 34 150821.0 35 174027.0 36 198018.5 37 230336.5 38 248305.0 39 245809.0 40 236846.5 41 225514.5 42 227408.0 43 239933.5 44 260030.5 45 299277.0 46 335725.0 47 359231.5 48 378872.5 49 380633.5 50 369554.5 51 375808.0 52 377221.0 53 356067.0 54 337462.5 55 300699.5 56 263278.0 57 222067.5 58 181690.5 59 170147.5 60 142276.0 61 101550.0 62 76522.0 63 62417.0 64 45449.5 65 25279.0 66 15542.5 67 11229.5 68 8420.5 69 6104.0 70 4582.0 71 3422.0 72 2518.5 73 1935.0 74 1437.5 75 995.5 76 637.0 77 352.5 78 201.0 79 126.5 80 88.0 81 57.0 82 30.5 83 23.0 84 20.5 85 11.0 86 8.5 87 6.5 88 4.0 89 3.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04443846839833992 2 0.0 3 0.0 4 0.033898652555694304 5 0.0 6 0.0 7 6.994385799467341E-4 8 0.0 9 0.01210752300459519 10-11 3.6177857583451765E-4 12-13 2.411857172230118E-5 14-15 1.5074107326438235E-4 16-17 3.4971928997336706E-4 18-19 0.0 20-21 2.411857172230118E-5 22-23 0.0 24-25 4.944307203071742E-4 26-27 4.160453622096953E-4 28-29 1.7485964498668353E-4 30-31 0.002381708957577241 32-33 0.015176611256258014 34-35 0.02601187960250182 36-37 0.031191342879865996 38-39 0.022008196696599824 40-41 0.0013988771598934682 42-43 7.838535809747882E-5 44-45 0.012258264077859573 46-47 0.018740130228228014 48-49 0.0036901414735120803 50-51 0.011739714785830097 52-53 0.01155882549791284 54-55 0.010401134055242383 56-57 4.2207500514027064E-4 58-59 0.00849576688918059 60-61 0.004685032557057003 62-63 0.00143505501747692 64-65 0.03669640687548124 66-67 0.061098371815519456 68-69 0.06056173359469826 70-71 0.0528618795723536 72-73 0.04664531771093048 74-75 0.03567739722021401 76-77 0.03190887038860446 78-79 0.01595443519430223 80-81 0.0013506400164488659 82-83 0.039090175118919636 84-85 0.05275937564253382 86-87 0.05951257572477816 88-89 0.07116184586664961 90-91 0.04933453845796706 92-93 0.040199629418145484 94-95 0.013572726236724987 96-97 0.003738378616956682 98-99 0.02216496741279478 100 0.032463597538217384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8292365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.6413989389224 #Duplication Level Percentage of deduplicated Percentage of total 1 75.11022836075438 47.050097791395785 2 14.713112950287139 18.432999559047243 3 4.936943702703156 9.277711800600873 4 2.1641831766683506 5.422698469863461 5 1.058730771863782 3.31601883246162 6 0.5876373495099548 2.208625538523843 7 0.36858896516453155 1.6162249887949194 8 0.232735672610375 1.1663110492243949 9 0.17193441514890523 0.9693191061605587 >10 0.613692587517811 6.299103238287074 >50 0.026781868135276665 1.1461984874370121 >100 0.01400400406335763 1.661091773212944 >500 0.0010508800423423394 0.4508902740736652 >1k 3.002364250158997E-4 0.29022689870752455 >5k 2.501970192424605E-5 0.15017572799536108 >10k+ 5.00394038484921E-5 0.5423064642136803 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18611 0.2244353691618736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15991 0.19284004020565906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9582 0.11555207712154493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2059285861150588E-5 2 0.0 0.0 0.0 0.0 1.2059285861150588E-5 3 0.0 0.0 0.0 0.0 1.2059285861150588E-5 4 0.0 0.0 0.0 0.0 1.2059285861150588E-5 5 0.0 0.0 0.0 0.0 1.2059285861150588E-5 6 0.0 0.0 0.0 0.0 1.2059285861150588E-5 7 0.0 0.0 0.0 0.0 1.2059285861150588E-5 8 0.0 0.0 0.0 0.0 1.2059285861150588E-5 9 0.0 0.0 0.0 0.0 1.2059285861150588E-5 10-11 0.0 0.0 0.0 0.0 1.2059285861150588E-5 12-13 0.0 0.0 0.0 0.0 1.2059285861150588E-5 14-15 0.0 0.0 0.0 0.0 1.2059285861150588E-5 16-17 0.0 0.0 0.0 1.8088928791725882E-5 1.2059285861150588E-5 18-19 0.0 0.0 0.0 3.014821465287647E-5 1.2059285861150588E-5 20-21 0.0 0.0 0.0 6.029642930575294E-5 1.2059285861150588E-5 22-23 0.0 0.0 0.0 2.4118571722301176E-4 1.8088928791725882E-5 24-25 0.0 0.0 0.0 5.848753642658036E-4 2.4118571722301176E-5 26-27 0.0 0.0 0.0 0.0011576914426704565 2.4118571722301176E-5 28-29 0.0 0.0 0.0 0.003521311471455972 2.4118571722301176E-5 30-31 6.029642930575294E-6 0.0 0.0 0.015309263400730673 2.4118571722301176E-5 32-33 1.2059285861150588E-5 0.0 0.0 0.04370285196080973 4.823714344460235E-5 34-35 1.2059285861150588E-5 0.0 0.0 0.08707407356043782 6.029642930575294E-5 36-37 1.2059285861150588E-5 0.0 0.0 0.1606176283846647 6.029642930575294E-5 38-39 1.2059285861150588E-5 0.0 0.0 0.2671614189679301 6.029642930575294E-5 40-41 1.2059285861150588E-5 0.0 0.0 0.3777571296005422 6.029642930575294E-5 42-43 1.2059285861150588E-5 0.0 0.0 0.4867067477131072 6.029642930575294E-5 44-45 1.2059285861150588E-5 0.0 0.0 0.6156808099981127 6.029642930575294E-5 46-47 2.4118571722301176E-5 0.0 0.0 0.7715832576110675 6.029642930575294E-5 48-49 2.4118571722301176E-5 0.0 0.0 0.9410885796754003 6.029642930575294E-5 50-51 2.4118571722301176E-5 0.0 0.0 1.113373567130728 6.029642930575294E-5 52-53 2.4118571722301176E-5 0.0 0.0 1.2773858844853065 6.632607223632824E-5 54-55 2.4118571722301176E-5 0.0 0.0 1.4521852330426843 7.838535809747882E-5 56-57 2.4118571722301176E-5 0.0 0.0 1.6607505820112838 9.64742868892047E-5 58-59 2.4118571722301176E-5 0.0 0.0 1.8980712981157968 9.64742868892047E-5 60-61 2.4118571722301176E-5 0.0 0.0 2.1383163910416387 9.64742868892047E-5 62-63 2.4118571722301176E-5 0.0 0.0 2.3771867253793095 9.64742868892047E-5 64-65 2.4118571722301176E-5 0.0 0.0 2.6196085194030894 9.64742868892047E-5 66-67 3.014821465287647E-5 0.0 0.0 2.894204488104419 9.64742868892047E-5 68-69 3.6177857583451765E-5 0.0 0.0 3.1936184671079966 9.64742868892047E-5 70-71 3.6177857583451765E-5 0.0 0.0 3.517844426770891 1.0250392981978E-4 72-73 3.6177857583451765E-5 0.0 0.0 3.8466348261322314 1.2059285861150588E-4 74-75 3.6177857583451765E-5 0.0 0.0 4.1592778417254905 1.2059285861150588E-4 76-77 3.6177857583451765E-5 0.0 0.0 4.5069651420312535 1.2059285861150588E-4 78-79 3.6177857583451765E-5 0.0 0.0 4.885445828783466 1.2059285861150588E-4 80-81 3.6177857583451765E-5 0.0 0.0 5.288925415125842 1.2059285861150588E-4 82-83 3.6177857583451765E-5 0.0 0.0 5.691729681459994 1.2059285861150588E-4 84-85 3.6177857583451765E-5 6.029642930575294E-6 0.0 6.081136081202407 1.2059285861150588E-4 86-87 3.6177857583451765E-5 1.2059285861150588E-5 0.0 6.489023336527034 1.2059285861150588E-4 88 3.6177857583451765E-5 1.2059285861150588E-5 0.0 6.822697746662141 1.2059285861150588E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14005 0.0 44.042854 1 GTATCAA 23275 0.0 36.601025 1 GTACTAG 7680 0.0 33.48504 1 GGGCACC 8460 0.0 32.173153 94 CTGTGCG 9870 0.0 31.22951 9 CAACGCA 25940 0.0 31.13717 5 ATCAACG 26530 0.0 30.400194 3 TAGTACT 10340 0.0 29.999895 4 TACCTGG 4975 0.0 29.944202 2 TAGGCAT 9380 0.0 28.95331 5 GTGGTAT 4980 0.0 28.415934 1 TATCAAC 29830 0.0 28.404625 2 AACGCAG 29765 0.0 27.893557 6 CTAGTAC 11790 0.0 27.267082 3 ACTGTGC 12405 0.0 27.19607 8 CTAGGCA 10625 0.0 27.071907 4 GTACTGT 12090 0.0 26.971582 6 GTACATG 34510 0.0 26.320063 1 TCAACGC 31350 0.0 26.071041 4 TACTGTG 13635 0.0 25.914087 7 >>END_MODULE