FastQCFastQC Report
Mon 6 Jun 2016
ERR1211219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6485424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195480.3014143716740802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155570.23987637508357204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99480.15339012530252455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124200.044.448111
TACCTGG40700.038.2745822
GTATCAA215100.037.7858161
GTACTAG62050.035.5719681
GCATCGT57950.035.2860578
CTGTGCG78300.032.958289
CAACGCA241000.032.2040675
GGGCACC70350.032.00160294
ATCAACG244500.031.8968833
ACCTGGG48200.031.599393
TAGTACT81750.031.3390674
TAGGCAT77100.030.6076535
TATCAAC268150.030.1173232
GTGGTAT44900.029.8727631
ACTGTGC93700.029.5490138
AACGCAG276200.028.967876
GTACATG312100.028.6206381
GTACCTG54750.028.5384791
TAACGCC28350.028.1885814
CTAGTAC95400.027.840743
Produced by FastQC (version 0.11.5)