##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211219_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6485424 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.126226442557957 31.0 31.0 34.0 28.0 34.0 2 31.13402870806905 31.0 31.0 34.0 27.0 34.0 3 31.191262437120535 31.0 31.0 34.0 27.0 34.0 4 34.78349125670118 37.0 35.0 37.0 32.0 37.0 5 34.84577816346317 37.0 35.0 37.0 32.0 37.0 6 34.90993618921446 37.0 35.0 37.0 32.0 37.0 7 34.811093461275625 37.0 35.0 37.0 31.0 37.0 8 34.817872663375596 37.0 35.0 37.0 31.0 37.0 9 36.32529947155344 39.0 37.0 39.0 31.0 39.0 10-11 36.26323529502466 39.0 36.0 39.0 31.0 39.0 12-13 36.14018219934425 39.0 35.0 39.0 30.0 39.0 14-15 37.26552103609571 40.0 36.5 41.0 31.0 41.0 16-17 37.24032268360558 40.0 36.0 41.0 31.0 41.0 18-19 37.25748509272485 40.0 36.5 41.0 31.0 41.0 20-21 37.225594116899686 40.0 36.5 41.0 31.0 41.0 22-23 37.108783866714035 40.0 36.0 41.0 30.0 41.0 24-25 36.99926835932392 40.0 36.0 41.0 30.0 41.0 26-27 36.84504567473152 39.0 36.0 41.0 30.0 41.0 28-29 36.716526089890195 39.0 36.0 41.0 30.0 41.0 30-31 36.56224396431136 39.0 35.5 41.0 29.5 41.0 32-33 36.37882927931929 39.0 35.0 40.5 29.0 41.0 34-35 36.226023387214155 39.0 35.0 40.0 28.5 41.0 36-37 36.11047758789556 39.0 35.0 40.0 28.0 41.0 38-39 35.96122759282971 38.0 35.0 40.0 27.0 41.0 40-41 35.79280013149487 38.0 35.0 40.0 27.0 41.0 42-43 35.57191519012481 38.0 34.0 40.0 26.5 41.0 44-45 35.327754206972436 38.0 34.0 40.0 26.0 41.0 46-47 35.08390345796975 38.0 33.5 40.0 25.0 41.0 48-49 34.856244865408954 38.0 33.0 40.0 24.0 41.0 50-51 34.185478389693564 37.5 32.5 39.5 23.5 40.5 52-53 34.30220252369005 37.5 33.0 39.5 24.0 40.5 54-55 34.77837169936769 38.0 33.0 40.0 24.5 41.0 56-57 34.7241445586287 37.5 33.0 40.0 24.5 41.0 58-59 34.495561970967515 37.0 33.0 40.0 24.0 41.0 60-61 34.275028664278544 37.0 33.0 40.0 24.0 41.0 62-63 33.96933415610144 36.0 33.0 40.0 23.5 41.0 64-65 33.72211230907956 36.0 32.0 39.0 23.0 41.0 66-67 33.55231708828906 35.0 32.0 39.0 23.0 41.0 68-69 33.25633829029529 35.0 32.0 39.0 22.5 41.0 70-71 32.97739083828598 35.0 32.0 38.0 22.0 40.5 72-73 32.65891836832873 35.0 31.0 37.0 22.0 40.0 74-75 32.33919355157042 35.0 31.0 37.0 21.0 39.5 76-77 32.02895377387816 35.0 31.0 36.0 20.0 39.0 78-79 31.700048292910378 35.0 31.0 36.0 20.0 39.0 80-81 31.40819767527921 35.0 30.0 35.5 20.0 37.5 82-83 31.118814436804747 34.0 30.0 35.0 19.5 37.0 84-85 30.876098386165655 34.0 30.0 35.0 19.0 37.0 86-87 30.659703359410273 34.0 29.5 35.0 18.0 36.0 88-89 30.45639853307972 34.0 29.0 35.0 18.0 36.0 90-91 30.220239416883153 34.0 29.0 35.0 18.0 36.0 92-93 30.018092957376417 34.0 29.0 35.0 17.0 35.0 94-95 29.848798706144734 34.0 29.0 35.0 17.0 35.0 96-97 29.664259499456012 34.0 29.0 35.0 15.5 35.0 98-99 29.419936460592247 34.0 27.5 35.0 15.0 35.0 100 28.244371840607492 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4476600307556211 1101 2 -0.3723408759578781 1101 3 -0.4564327520368927 1101 4 -0.4710664852264088 1101 5 -0.39705486464277584 1101 6 -0.37025693747512634 1101 7 -0.3926650100171045 1101 8 -0.37471330788651613 1101 9 -0.5515018850201656 1101 10-11 -0.5696305624159308 1101 12-13 -0.6091494557514778 1101 14-15 -0.6546330796569322 1101 16-17 -0.7054076666081315 1101 18-19 -0.6935141841987686 1101 20-21 -0.800265464558727 1101 22-23 -0.7912728612876592 1101 24-25 -0.943753590975895 1101 26-27 -0.887360523226846 1101 28-29 -0.8579081930814425 1101 30-31 -0.8765865538841027 1101 32-33 -0.8635080893667606 1101 34-35 -0.95897654367716 1101 36-37 -1.0293180322646194 1101 38-39 -1.0104339285061883 1101 40-41 -1.0045315661370182 1101 42-43 -1.020515427522028 1101 44-45 -1.0261327326769774 1101 46-47 -1.1060264125267523 1101 48-49 -1.3118370545042168 1101 50-51 -1.181650722781363 1101 52-53 -1.0223124893059818 1101 54-55 -0.9827299270083572 1101 56-57 -0.8701259169945743 1101 58-59 -0.86955453785815 1101 60-61 -0.8561493448335611 1101 62-63 -0.9088438406565444 1101 64-65 -0.818606054443201 1101 66-67 -0.8895229782067631 1101 68-69 -1.002063404303776 1101 70-71 -0.9270391731395549 1101 72-73 -1.0245176817979562 1101 74-75 -1.1237451278789194 1101 76-77 -1.135979240916999 1101 78-79 -1.1571945414639586 1101 80-81 -1.2612518155158163 1101 82-83 -1.3142309665932252 1101 84-85 -1.3291187258398054 1101 86-87 -1.3291240301746186 1101 88-89 -1.4670937027048758 1101 90-91 -1.460145979103853 1101 92-93 -1.5873446787595107 1101 94-95 -1.6148199022176009 1101 96-97 -1.5859265121502624 1101 98-99 -1.7035856033647185 1101 100 -1.777751393500111 1102 1 -0.5292133387788311 1102 2 -0.44604504404097156 1102 3 -0.4253580727919051 1102 4 -0.22619143498312155 1102 5 -0.35749456313185135 1102 6 -0.3668517615250124 1102 7 -0.3686156291248963 1102 8 -0.4120580075019902 1102 9 -0.5664857734715838 1102 10-11 -0.5599634086151823 1102 12-13 -0.6120086389567589 1102 14-15 -0.728593979351615 1102 16-17 -0.7737014653400749 1102 18-19 -0.7415041248485679 1102 20-21 -0.798343706684939 1102 22-23 -0.7186181297204612 1102 24-25 -0.8511644180767277 1102 26-27 -0.7565147008486406 1102 28-29 -0.7985039398195255 1102 30-31 -0.8735924450523598 1102 32-33 -0.9273173969974593 1102 34-35 -0.9381711288956538 1102 36-37 -0.8738458343571622 1102 38-39 -1.0922037555416892 1102 40-41 -1.1471596801085937 1102 42-43 -1.1817626489212785 1102 44-45 -1.2818305884153816 1102 46-47 -1.1615446010673196 1102 48-49 -1.1684885284465025 1102 50-51 -1.2506104331715662 1102 52-53 -1.1684240927031766 1102 54-55 -1.1413295868142512 1102 56-57 -1.01504691294069 1102 58-59 -0.9737512684974448 1102 60-61 -0.8860831544458208 1102 62-63 -0.9886273000777166 1102 64-65 -1.0713829478433397 1102 66-67 -1.1158713426053382 1102 68-69 -1.218524860909259 1102 70-71 -1.1672133480601374 1102 72-73 -1.12348762820422 1102 74-75 -1.1465723153342466 1102 76-77 -1.173324165183871 1102 78-79 -1.160623305810308 1102 80-81 -1.2700073339649514 1102 82-83 -1.1963991993946124 1102 84-85 -1.1590474223863652 1102 86-87 -1.2637931586611053 1102 88-89 -1.3889515014285472 1102 90-91 -1.4083261365808823 1102 92-93 -1.4151456121051709 1102 94-95 -1.4683272451280978 1102 96-97 -1.56364131864499 1102 98-99 -1.6528847943591067 1102 100 -1.6723089259579709 1103 1 -0.64830455622279 1103 2 -0.5809716744448785 1103 3 -0.6508113990957725 1103 4 -0.46526884611719055 1103 5 -0.4767266524611813 1103 6 -0.38748079818221015 1103 7 -0.5255421223769687 1103 8 -0.5737239961956746 1103 9 -0.7113123955068019 1103 10-11 -0.6875729485457711 1103 12-13 -0.6923970577824505 1103 14-15 -0.9852187501139795 1103 16-17 -0.9739625873123998 1103 18-19 -0.8361151249443637 1103 20-21 -0.8729094016903005 1103 22-23 -0.9323759029791887 1103 24-25 -0.9866575639746102 1103 26-27 -1.0208595969809267 1103 28-29 -1.0005397159146057 1103 30-31 -1.024685414786859 1103 32-33 -1.0974364341731615 1103 34-35 -1.2245559721996884 1103 36-37 -1.1510935714529111 1103 38-39 -1.1472974522154828 1103 40-41 -1.1379237040417607 1103 42-43 -1.1983328306024603 1103 44-45 -1.1846830292536623 1103 46-47 -1.3550104929703366 1103 48-49 -1.330969382414466 1103 50-51 -1.3821788623891962 1103 52-53 -1.2033358082781902 1103 54-55 -1.0332434915700972 1103 56-57 -1.0387615918026825 1103 58-59 -1.0074674474936174 1103 60-61 -1.1166608866293544 1103 62-63 -1.0014314586273017 1103 64-65 -1.0541030429337255 1103 66-67 -1.0903924776849507 1103 68-69 -1.0542955662359006 1103 70-71 -0.9798421910437298 1103 72-73 -1.0861099336941749 1103 74-75 -1.083245356273487 1103 76-77 -1.1047738150343278 1103 78-79 -1.209705464197139 1103 80-81 -1.227101131685842 1103 82-83 -1.1763026209751715 1103 84-85 -1.202020816373338 1103 86-87 -1.3263009556387964 1103 88-89 -1.3603716670961923 1103 90-91 -1.534238898443263 1103 92-93 -1.5954053198794966 1103 94-95 -1.525784777805903 1103 96-97 -1.6008982122690973 1103 98-99 -1.628784498536799 1103 100 -1.7962295546139693 1104 1 -0.662578063853605 1104 2 -0.6035790501876406 1104 3 -0.6856342221833209 1104 4 -0.5032039665474315 1104 5 -0.4970356030108647 1104 6 -0.597930494089205 1104 7 -0.6036509541258752 1104 8 -0.5445074990100593 1104 9 -0.629545563854272 1104 10-11 -0.6494114797369193 1104 12-13 -0.5868899951649382 1104 14-15 -0.8224989875054476 1104 16-17 -0.9626599556763651 1104 18-19 -0.9834236717266833 1104 20-21 -0.9354795556996862 1104 22-23 -0.8346961437140621 1104 24-25 -1.0801921419223746 1104 26-27 -1.0777535708214572 1104 28-29 -1.045428319055361 1104 30-31 -1.0676995998561836 1104 32-33 -1.082755704998526 1104 34-35 -1.1589549366094687 1104 36-37 -1.1882529298464632 1104 38-39 -1.119838930291806 1104 40-41 -1.1837111920035355 1104 42-43 -1.1156758209337454 1104 44-45 -1.1830188876555496 1104 46-47 -1.275027899728741 1104 48-49 -1.0970944991427416 1104 50-51 -1.245828443240761 1104 52-53 -1.1202464272458101 1104 54-55 -0.9560913806637856 1104 56-57 -1.018336969209841 1104 58-59 -0.989225520956893 1104 60-61 -0.9901900360984186 1104 62-63 -1.0695753094306966 1104 64-65 -0.9671390481123652 1104 66-67 -1.0077134012617037 1104 68-69 -1.080452834767918 1104 70-71 -1.096390281495843 1104 72-73 -0.9590650049826372 1104 74-75 -1.0611350391722283 1104 76-77 -0.987350191126275 1104 78-79 -1.0873283534678215 1104 80-81 -1.1633573289325945 1104 82-83 -1.0999461122017955 1104 84-85 -1.2102513732502977 1104 86-87 -1.2332417406877099 1104 88-89 -1.306650995325949 1104 90-91 -1.3360481268113915 1104 92-93 -1.4550885942958303 1104 94-95 -1.4642716869963799 1104 96-97 -1.4801095082093383 1104 98-99 -1.4891764497722981 1104 100 -1.2853003164439762 1105 1 -0.45578508572265974 1105 2 -0.45905473522403284 1105 3 -0.5365292027216739 1105 4 -0.39526473868935597 1105 5 -0.3101252547168585 1105 6 -0.4256826137905847 1105 7 -0.33613033662659575 1105 8 -0.4324252906025521 1105 9 -0.5303155043201215 1105 10-11 -0.6315160104207749 1105 12-13 -0.5585554864483839 1105 14-15 -0.6838340464144821 1105 16-17 -0.7386166077585798 1105 18-19 -0.7204122573683662 1105 20-21 -0.7245970895582872 1105 22-23 -0.7184504783846251 1105 24-25 -0.6779690139361279 1105 26-27 -0.7193742813316675 1105 28-29 -0.8076331461574355 1105 30-31 -0.8388142752766754 1105 32-33 -0.9407713693224622 1105 34-35 -0.8491242027605281 1105 36-37 -0.9055114122595214 1105 38-39 -0.8425030163102321 1105 40-41 -0.8887777911525276 1105 42-43 -0.8870954774201465 1105 44-45 -0.9190883886485182 1105 46-47 -1.0051845776005095 1105 48-49 -0.952398638642741 1105 50-51 -0.9142094637704687 1105 52-53 -0.8922560456862811 1105 54-55 -0.7567338510210959 1105 56-57 -0.668495100485579 1105 58-59 -0.6361009679305667 1105 60-61 -0.6531500560605039 1105 62-63 -0.7398820757938722 1105 64-65 -0.6281043028684579 1105 66-67 -0.6638251785323348 1105 68-69 -0.7507537857835871 1105 70-71 -0.6766928423382268 1105 72-73 -0.6056503627976539 1105 74-75 -0.71368819895212 1105 76-77 -0.6577750249170045 1105 78-79 -0.8033484987551169 1105 80-81 -0.8142714265239981 1105 82-83 -0.8427018919645697 1105 84-85 -0.8705686857389594 1105 86-87 -0.9057892628994502 1105 88-89 -0.8971951046357809 1105 90-91 -0.9603464466925615 1105 92-93 -1.071937526170899 1105 94-95 -1.134366994048463 1105 96-97 -1.0849272220291333 1105 98-99 -1.0029767744620521 1105 100 -1.1456911678258592 1106 1 -0.5792069025340467 1106 2 -0.5369923944640966 1106 3 -0.6036400946075062 1106 4 -0.4725734200189464 1106 5 -0.4687510094220144 1106 6 -0.49547512322419607 1106 7 -0.45638849515545843 1106 8 -0.5130285886066162 1106 9 -0.6300067136189966 1106 10-11 -0.6626256174658423 1106 12-13 -0.7313728670139525 1106 14-15 -1.0103801353563355 1106 16-17 -0.9991057281936051 1106 18-19 -1.0055244017992138 1106 20-21 -0.8662839807528826 1106 22-23 -0.864305634006854 1106 24-25 -0.8614915737384194 1106 26-27 -0.9831519171948031 1106 28-29 -0.9827225153260386 1106 30-31 -1.100434817601105 1106 32-33 -1.0338951153084963 1106 34-35 -1.0559734478390794 1106 36-37 -1.0922547819731037 1106 38-39 -1.0921364694919617 1106 40-41 -1.1826909341474519 1106 42-43 -1.0561357919479946 1106 44-45 -1.112868735832258 1106 46-47 -1.0108980918724768 1106 48-49 -1.0787254096182721 1106 50-51 -1.062435129738411 1106 52-53 -1.0345358568480592 1106 54-55 -0.8361789549230636 1106 56-57 -0.8775862083825885 1106 58-59 -0.9015400873130233 1106 60-61 -0.8905422954385784 1106 62-63 -0.8897253329429802 1106 64-65 -0.8775492937635079 1106 66-67 -0.9707527309239339 1106 68-69 -0.9298462630088196 1106 70-71 -0.9029592405650888 1106 72-73 -0.829103959634562 1106 74-75 -0.8067626277042592 1106 76-77 -0.8425146635986565 1106 78-79 -0.8884557424619786 1106 80-81 -0.8896280612827887 1106 82-83 -0.9618594871018118 1106 84-85 -0.8982476873597527 1106 86-87 -1.0016797902508863 1106 88-89 -0.9416146017617528 1106 90-91 -1.0786217565154033 1106 92-93 -1.1996350485266092 1106 94-95 -1.2477786241738293 1106 96-97 -1.0760395942210543 1106 98-99 -1.1809578103229263 1106 100 -1.262981443400136 1107 1 -0.6216120404899215 1107 2 -0.6711177904164387 1107 3 -0.5936899676074852 1107 4 -0.6441304972400417 1107 5 -0.4523639350643407 1107 6 -0.5430055273513688 1107 7 -0.5230505473851181 1107 8 -0.5560982604253795 1107 9 -0.7006932141247333 1107 10-11 -0.6445081017633711 1107 12-13 -0.7498174034724627 1107 14-15 -0.8808449506174867 1107 16-17 -0.9015410247913493 1107 18-19 -0.9568326290245253 1107 20-21 -0.8909024141927233 1107 22-23 -0.8605330845510082 1107 24-25 -0.9235497362448868 1107 26-27 -0.9120198763158314 1107 28-29 -0.9967822791520078 1107 30-31 -1.0163253730433581 1107 32-33 -1.0621635821549376 1107 34-35 -1.0537966283809865 1107 36-37 -1.0641386739485839 1107 38-39 -1.0385825907898507 1107 40-41 -1.1148269584117045 1107 42-43 -1.0785061430915448 1107 44-45 -1.1033639844041616 1107 46-47 -0.9704569324677124 1107 48-49 -0.8854753558861717 1107 50-51 -1.0236144205296611 1107 52-53 -0.9834178378148763 1107 54-55 -0.9381184742113433 1107 56-57 -0.9660574769888299 1107 58-59 -0.9770214136065931 1107 60-61 -0.9320881455696295 1107 62-63 -0.8937541347206306 1107 64-65 -0.9093714951108964 1107 66-67 -0.9013839131193535 1107 68-69 -0.8968612098020259 1107 70-71 -0.9115036260243912 1107 72-73 -0.9079188595967267 1107 74-75 -0.7563663835263768 1107 76-77 -0.9653499604710731 1107 78-79 -0.7643506651392578 1107 80-81 -0.8562634156983329 1107 82-83 -0.8266368851498775 1107 84-85 -0.964193650379709 1107 86-87 -0.9002347985287216 1107 88-89 -0.884912825546845 1107 90-91 -1.0815121656964841 1107 92-93 -1.1216308337328087 1107 94-95 -1.1674993408673124 1107 96-97 -1.1015445351706816 1107 98-99 -1.0639178205955417 1107 100 -1.0294035037050584 1108 1 -0.7046014637721711 1108 2 -0.7226164967035267 1108 3 -0.7100727180121709 1108 4 -0.5245604370104786 1108 5 -0.542971828565598 1108 6 -0.5671180726279559 1108 7 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1.0123401066447073 2311 10-11 1.0052471668063987 2311 12-13 1.1354482126763301 2311 14-15 1.331255093245936 2311 16-17 1.319767350476738 2311 18-19 1.2616957642738456 2311 20-21 1.2848591892246546 2311 22-23 1.3417302017669144 2311 24-25 1.3710976812136337 2311 26-27 1.3342079136952094 2311 28-29 1.4135931939226793 2311 30-31 1.3729470452417303 2311 32-33 1.4472347285523597 2311 34-35 1.4596381418650637 2311 36-37 1.4803744531164682 2311 38-39 1.586609471474162 2311 40-41 1.5412542197729735 2311 42-43 1.5830853275673533 2311 44-45 1.6333729739432314 2311 46-47 1.670969851990968 2311 48-49 1.7151345109585918 2311 50-51 1.6952036697143669 2311 52-53 1.593933252488661 2311 54-55 1.4643368964125116 2311 56-57 1.442567812586887 2311 58-59 1.3625340119016442 2311 60-61 1.3655156112550983 2311 62-63 1.4684597005512856 2311 64-65 1.441810359540277 2311 66-67 1.4096646320037678 2311 68-69 1.4079465185357307 2311 70-71 1.359043950356373 2311 72-73 1.3511737407105358 2311 74-75 1.3617234747661229 2311 76-77 1.4137853350339213 2311 78-79 1.457902636189207 2311 80-81 1.393960089363592 2311 82-83 1.3833655504822069 2311 84-85 1.5127970876057901 2311 86-87 1.4943104656547028 2311 88-89 1.5483749691811717 2311 90-91 1.5092811450218377 2311 92-93 1.6090103216164344 2311 94-95 1.6461044688580238 2311 96-97 1.6591627789994057 2311 98-99 1.7752471323118968 2311 100 1.8174419008853917 2312 1 0.6862506222838611 2312 2 0.6610693817841735 2312 3 0.7134931824151529 2312 4 0.42465583287284403 2312 5 0.5681118342211491 2312 6 0.5897320367613901 2312 7 0.43754623887574695 2312 8 0.6011116518224355 2312 9 0.7875793483461564 2312 10-11 0.7665043997175616 2312 12-13 0.8975959547026164 2312 14-15 1.1130154584382765 2312 16-17 1.098876545986279 2312 18-19 1.082430958065764 2312 20-21 1.1272345519219868 2312 22-23 1.16983339077143 2312 24-25 1.14145118071432 2312 26-27 1.2209576119300465 2312 28-29 1.1940727866553615 2312 30-31 1.2517861941129453 2312 32-33 1.221919120532263 2312 34-35 1.1951556848717502 2312 36-37 1.2100291301261805 2312 38-39 1.25359845752984 2312 40-41 1.2407199090230492 2312 42-43 1.3047731085831913 2312 44-45 1.3394289921752431 2312 46-47 1.3469007102925588 2312 48-49 1.375572844791023 2312 50-51 1.3927729982491215 2312 52-53 1.2577330298340925 2312 54-55 1.1255875124232304 2312 56-57 1.0737836591533707 2312 58-59 1.0774552244909827 2312 60-61 1.0832893521974754 2312 62-63 1.0685839218992967 2312 64-65 1.1178139703013485 2312 66-67 1.0875099203164993 2312 68-69 1.0965015744215165 2312 70-71 1.090735388182253 2312 72-73 1.0583407962000493 2312 74-75 1.0283486369145152 2312 76-77 1.1257779750831958 2312 78-79 1.1570070187354027 2312 80-81 1.1141217233954528 2312 82-83 1.1202782314770552 2312 84-85 1.1551603342813763 2312 86-87 1.2017179404743956 2312 88-89 1.1107372931168733 2312 90-91 1.218534574878717 2312 92-93 1.232964300378562 2312 94-95 1.3051850882998899 2312 96-97 1.3420650057492303 2312 98-99 1.0038542390354799 2312 100 1.2594215063977217 2313 1 0.710274483834322 2313 2 0.6853484712766011 2313 3 0.6982259047922774 2313 4 0.6657569609834084 2313 5 0.45660355257107454 2313 6 0.6188217630577384 2313 7 0.6655543062285787 2313 8 0.6292966846987227 2313 9 0.8125552376737701 2313 10-11 0.81182276051738 2313 12-13 0.934278559197125 2313 14-15 1.1564873881185065 2313 16-17 1.1217124053182275 2313 18-19 1.046511443617284 2313 20-21 1.1211817836895577 2313 22-23 1.159141158199965 2313 24-25 1.1716197153475676 2313 26-27 1.17244472629514 2313 28-29 1.1920262432905915 2313 30-31 1.2127221935209178 2313 32-33 1.2841107084091163 2313 34-35 1.3310724473622813 2313 36-37 1.3776417724169079 2313 38-39 1.3359296917950587 2313 40-41 1.4112789747234586 2313 42-43 1.4603586316039383 2313 44-45 1.4668737126451177 2313 46-47 1.4541678386497807 2313 48-49 1.4300390420816171 2313 50-51 1.4809759123889066 2313 52-53 1.3582386672403715 2313 54-55 1.1808119183511039 2313 56-57 1.1214265484894739 2313 58-59 1.1451842177949985 2313 60-61 1.1060135259182786 2313 62-63 1.15463633737766 2313 64-65 1.1679267248899876 2313 66-67 1.1174933526111843 2313 68-69 1.141569860822365 2313 70-71 1.1499444608399472 2313 72-73 1.1340302956078432 2313 74-75 1.1263919948114207 2313 76-77 1.0054674249625464 2313 78-79 1.1824081348907747 2313 80-81 1.2418536645941067 2313 82-83 1.2787714668055337 2313 84-85 1.322766113685617 2313 86-87 1.2443687814892428 2313 88-89 1.2813235127163516 2313 90-91 1.3553159420075467 2313 92-93 1.489593750924307 2313 94-95 1.432958759590214 2313 96-97 1.498525619082418 2313 98-99 1.5451073728531064 2313 100 1.6413725537600925 2314 1 0.6166755452966015 2314 2 0.6338851625663899 2314 3 0.6177639421733616 2314 4 0.48652367530890217 2314 5 0.5275855326091801 2314 6 0.5366557797304168 2314 7 0.5875457024846042 2314 8 0.5543424130442034 2314 9 0.7075267530924236 2314 10-11 0.7773091670275463 2314 12-13 0.8407632445414563 2314 14-15 0.9864898165706038 2314 16-17 0.932590624552212 2314 18-19 0.8882980128307665 2314 20-21 0.9458451673617176 2314 22-23 1.0237868608740825 2314 24-25 0.9821586480155631 2314 26-27 1.0396738409368567 2314 28-29 1.0301162993104782 2314 30-31 1.0757508423380457 2314 32-33 1.009785418094566 2314 34-35 1.0678864770831353 2314 36-37 1.1228699070721078 2314 38-39 1.1386203501915446 2314 40-41 1.15762505956517 2314 42-43 1.2895762814494773 2314 44-45 1.345490321423803 2314 46-47 1.2918048312999701 2314 48-49 1.2431173819868633 2314 50-51 1.2741401755433444 2314 52-53 1.1786558509502072 2314 54-55 1.0166182049936552 2314 56-57 1.0400017279481801 2314 58-59 1.0918935625958426 2314 60-61 1.0779963065809923 2314 62-63 1.0708233044905526 2314 64-65 1.0633582295826685 2314 66-67 1.0703873396717754 2314 68-69 1.106903922261047 2314 70-71 1.1014161633821402 2314 72-73 1.167660161907932 2314 74-75 1.1419499425208244 2314 76-77 1.1946703472076265 2314 78-79 1.1551802369575235 2314 80-81 1.1935807236571954 2314 82-83 1.2261004193926617 2314 84-85 1.262980234513968 2314 86-87 1.3119771593146865 2314 88-89 1.3295841409344327 2314 90-91 1.3875677353864866 2314 92-93 1.4268686404151687 2314 94-95 1.4685504524238873 2314 96-97 1.462291910638136 2314 98-99 1.5863057217837095 2314 100 1.700661588247982 2315 1 0.40534808525427124 2315 2 0.45791188915831427 2315 3 0.43695709454901177 2315 4 0.20027376026693844 2315 5 0.26186767600164984 2315 6 0.31483650755565407 2315 7 0.35682882884646006 2315 8 0.36834272143698144 2315 9 0.597536588004445 2315 10-11 0.4177283170453805 2315 12-13 0.44830371555012505 2315 14-15 0.6826825811071089 2315 16-17 0.6814478655665184 2315 18-19 0.47431721054250175 2315 20-21 0.5937352108537084 2315 22-23 0.47452357588301197 2315 24-25 0.6545978138209065 2315 26-27 0.6803276284216082 2315 28-29 0.6999942982639809 2315 30-31 0.7138017666749761 2315 32-33 0.7635165109360571 2315 34-35 0.814179681261983 2315 36-37 0.8517123552777051 2315 38-39 0.8991750410558268 2315 40-41 0.9110975929740661 2315 42-43 0.9650071605685042 2315 44-45 0.9693150468283349 2315 46-47 1.041416649709852 2315 48-49 1.088051955492297 2315 50-51 0.9864931818316123 2315 52-53 1.0197454617530326 2315 54-55 0.9178826317163882 2315 56-57 0.8697203658435271 2315 58-59 0.8988762555510377 2315 60-61 0.8041640605148572 2315 62-63 0.7784556117134329 2315 64-65 0.7959449953837918 2315 66-67 0.838048957906814 2315 68-69 0.8590457453711764 2315 70-71 0.8407976479959629 2315 72-73 0.6137694849322912 2315 74-75 0.8096304308981104 2315 76-77 0.861197950122957 2315 78-79 0.9191778319112522 2315 80-81 0.6081162155051985 2315 82-83 0.6827344778273563 2315 84-85 0.9968021042848108 2315 86-87 1.051544328606802 2315 88-89 1.146064734822751 2315 90-91 1.1964950868390716 2315 92-93 1.2192500471480727 2315 94-95 1.2061095300581215 2315 96-97 1.0886472603984707 2315 98-99 0.5299626111989362 2315 100 1.079517492763106 2316 1 0.23534245964105693 2316 2 0.211700146731296 2316 3 0.2687199114707717 2316 4 0.15276396452722452 2316 5 0.09204791290043346 2316 6 0.17255803472770737 2316 7 0.13345729858542654 2316 8 0.18383183565290295 2316 9 0.25883102092483057 2316 10-11 0.17663567976627803 2316 12-13 -0.0056237491176744925 2316 14-15 0.018325539745106312 2316 16-17 0.26119224178574996 2316 18-19 0.2607011716024701 2316 20-21 0.2827227981174687 2316 22-23 0.19472130741796434 2316 24-25 0.2734044364425188 2316 26-27 0.3226193864924909 2316 28-29 0.2794074923557517 2316 30-31 0.17548499910154192 2316 32-33 0.4568122799723042 2316 34-35 0.4649059979277368 2316 36-37 0.45230520433536014 2316 38-39 0.6245942984539425 2316 40-41 0.6762032741366113 2316 42-43 0.6030285638147674 2316 44-45 0.6075820634575351 2316 46-47 0.3973675746768919 2316 48-49 0.27588601717980055 2316 50-51 0.4158628422110624 2316 52-53 0.6045404881245702 2316 54-55 0.3549757302486469 2316 56-57 0.19569821207920057 2316 58-59 0.16909168329135582 2316 60-61 0.3296570019278491 2316 62-63 0.46614314306136606 2316 64-65 0.46012278413298446 2316 66-67 0.3422639139228423 2316 68-69 0.3504485243560467 2316 70-71 0.1388637551030314 2316 72-73 0.06392793272377162 2316 74-75 0.25164119626423087 2316 76-77 0.3506967181948113 2316 78-79 0.450701651893727 2316 80-81 0.5883938142557845 2316 82-83 0.5850791726829989 2316 84-85 0.6609398409715546 2316 86-87 0.7421408046012168 2316 88-89 0.6991973623846413 2316 90-91 0.5767070905207206 2316 92-93 0.6191890004751173 2316 94-95 0.7097063296103734 2316 96-97 0.6977729984820691 2316 98-99 0.5578734013816238 2316 100 0.5645289246164857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 30.0 1 424.0 2 158.0 3 131.0 4 110.0 5 77.0 6 100.0 7 435.0 8 1454.0 9 2794.0 10 5040.0 11 9002.0 12 14064.0 13 18878.0 14 21865.0 15 25497.0 16 29800.0 17 35285.0 18 41685.0 19 49268.0 20 58628.0 21 69707.0 22 75541.0 23 76777.0 24 76173.0 25 80045.0 26 88511.0 27 100231.0 28 116699.0 29 137977.0 30 164978.0 31 200814.0 32 251655.0 33 325701.0 34 454343.0 35 614557.0 36 899804.0 37 1182075.0 38 985570.0 39 269347.0 40 194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70789621508139 20.52812581044844 12.721994411695233 23.041983562774934 2 15.58750046704341 19.708845910259114 41.55065205435798 23.15300156833949 3 19.418696141512324 26.48632384968495 28.333493205337028 25.761486803465694 4 12.334627979942617 16.062207808130132 39.30210914048265 32.3010550714446 5 12.931635279379575 38.492093173459764 34.56206749722826 14.014204049932397 6 29.97948490417238 37.73927593343841 18.14678861151462 14.1344505508746 7 27.549218947310283 31.968259566431712 22.69637244914568 17.786149037112324 8 26.481624721816928 35.305084259427524 19.734946643460706 18.478344375294846 9 26.589925194418157 15.261526453441066 21.40773939675804 36.74080895538274 10-11 25.966688693832268 26.190855209332113 27.0631494774564 20.779306619379216 12-13 25.841824573408267 23.87627214346992 27.83644619457755 22.445457088544263 14-15 23.239219569625604 25.065037021192232 25.6932572087052 26.00248620047696 16-17 21.759118640936354 27.83538009781689 26.938184219521784 23.46731704172497 18-19 21.17291881979739 27.810184031037004 29.287631070286434 21.72926607887917 20-21 22.705619889224817 26.953076352859682 28.801940952340853 21.539362805574648 22-23 22.996374479680693 26.75316124488896 28.445821320625893 21.80464295480446 24-25 22.321388292770873 26.697670372773207 29.26859719193007 21.712344142525847 26-27 22.293431195807226 26.95834637464613 28.309607590078752 22.438614839467895 28-29 21.556328030961392 27.328145855968465 28.88855303001679 22.22697308305336 30-31 22.48991050831576 27.028911398999327 28.8092838359708 21.671894256714115 32-33 22.6093987993631 27.176300900755283 28.05959753802192 22.1547027618597 34-35 22.666486631377282 27.11433248497744 28.64110319785241 21.57807768579287 36-37 22.2663939097308 26.749856606739414 28.32041958587687 22.663329897652915 38-39 22.456515360594874 26.994818433099415 28.761470949642913 21.7871952566628 40-41 22.275364053374858 26.077701015546122 29.26445008653873 22.382484844540286 42-43 22.229259063772396 26.27513018756696 28.64272231372173 22.852888434938915 44-45 22.323170208274508 27.076488101183344 28.503579451766463 22.096762238775682 46-47 22.302159660654507 26.937723770706384 27.85596945310513 22.904147115533984 48-49 22.855819807209983 26.881097861721916 27.930118459355068 22.332963871713034 50-51 22.573059575883757 26.67484056426884 27.957344068855072 22.794755790992323 52-53 22.22726371050864 27.214874143782524 27.682953578921943 22.874908566786893 54-55 22.026995437415845 27.16555773791028 27.77972773190308 23.027719092770795 56-57 22.88890683339322 26.778247034278607 27.33224632827291 23.00059980405527 58-59 22.063248592217985 27.114326268835633 28.076474867092838 22.745950271853545 60-61 22.158575079247903 27.7163193809059 27.483420344666204 22.64168519517999 62-63 22.445802419848317 27.027924488492662 27.21865505837012 23.307618033288897 64-65 21.8160023973629 27.11806256555771 27.58773378105494 23.478201256024448 66-67 22.2266986387692 26.868805151279183 27.428838794325877 23.47565741562574 68-69 21.881359253836752 26.89666523187182 27.72731658374241 23.494658930549015 70-71 21.91430623883493 26.48808876495123 27.808862458041077 23.788742538172762 72-73 22.382717117777517 26.602929432277683 27.959331763272893 23.055021686671903 74-75 22.56701663047189 26.837776534140655 27.539107670570246 23.056099164817212 76-77 22.280957495306968 26.70193661895805 27.310758157975744 23.706347727759233 78-79 22.222758576177654 26.730355186012822 27.330314208913343 23.71657202889618 80-81 21.850652142312658 27.147974825035693 27.752672131292922 23.248700901358728 82-83 22.65630723158114 26.314416879637225 28.6578050555569 22.371470833224734 84-85 22.70084995324301 26.665979910532535 27.6185610443957 23.014609091828753 86-87 21.617139169196438 27.242817234165763 28.014032041292662 23.126011555345137 88-89 22.59036258386062 26.154497322837145 27.856928765190876 23.398211328111355 90-91 22.326372031247203 26.29633765142575 27.905327792751304 23.471962524575744 92-93 22.625406270427263 26.37286805619654 27.817486232358963 23.184239441017233 94-95 21.647894308775694 26.392723726583906 28.395162548407637 23.564219416232763 96-97 21.937137717747216 26.22381583293214 28.04822196707383 23.790824482246816 98-99 21.44129140282213 26.581655977158427 27.9084773064907 24.068575313528747 100 22.157188610754865 26.136344882420488 27.15431592731378 24.552150579510865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 865.0 1 621.5 2 654.5 3 939.0 4 795.0 5 703.0 6 1006.0 7 1810.0 8 2827.0 9 3339.0 10 3165.0 11 3009.0 12 3353.0 13 3842.0 14 4415.0 15 5181.0 16 5877.5 17 6546.5 18 7305.0 19 7935.0 20 8618.5 21 9407.0 22 10416.0 23 11813.5 24 13503.0 25 15854.0 26 19408.0 27 23728.5 28 29185.0 29 39604.5 30 55226.0 31 70855.0 32 91243.0 33 115117.5 34 134644.5 35 154294.0 36 174518.0 37 201344.5 38 214390.5 39 208324.5 40 198092.0 41 185661.5 42 182695.0 43 190225.5 44 204294.5 45 232327.0 46 260164.0 47 278678.5 48 292739.5 49 292571.0 50 282912.0 51 287366.5 52 285612.5 53 267360.5 54 248903.0 55 215459.0 56 186735.5 57 154124.0 58 122111.5 59 113618.5 60 94914.5 61 67279.0 62 50452.0 63 41806.5 64 30810.0 65 16618.5 66 9906.5 67 6744.5 68 4943.5 69 3749.0 70 2772.0 71 2015.0 72 1546.5 73 1273.5 74 985.5 75 714.5 76 489.0 77 350.0 78 260.5 79 187.5 80 138.5 81 114.5 82 107.5 83 84.5 84 50.0 85 35.5 86 34.5 87 27.5 88 20.0 89 18.5 90 18.0 91 17.0 92 12.0 93 8.0 94 4.5 95 4.0 96 2.5 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056634693429450406 2 0.13121732673145195 3 0.01649853579349631 4 0.015866348907951123 5 0.018765157066060755 6 0.05193183976868745 7 0.05740565304596893 8 0.04906386999523855 9 0.04038286471324003 10-11 0.03885636467253337 12-13 0.04032889754008373 14-15 0.019004154547181493 16-17 0.01797106866104668 18-19 0.01795564946871631 20-21 0.021440386935380015 22-23 0.02567295523006669 24-25 0.024292937516498535 26-27 0.01884996262387779 28-29 0.018795995450721498 30-31 0.016629598928304457 32-33 0.023622202650127426 34-35 0.023282980418859275 36-37 0.023236722841868167 38-39 0.021301614204406683 40-41 0.025009929959860757 42-43 0.032164435201152614 44-45 0.040976503617959285 46-47 0.047228986107924475 48-49 0.03958877630822595 50-51 0.027399904771068167 52-53 0.039318940442444474 54-55 0.03647409945749114 56-57 0.03813937222917114 58-59 0.043135190544211145 60-61 0.02310565970706002 62-63 0.02575776078788372 64-65 0.025179541075494832 66-67 0.03181750337371928 68-69 0.03854027122976077 70-71 0.04001280409731114 72-73 0.044268501180493365 74-75 0.027037553751304465 76-77 0.020661717722696312 78-79 0.020268528318271865 80-81 0.024693836517088164 82-83 0.020831328838330384 84-85 0.012828768018868158 86-87 0.015095389291432603 88-89 0.018927058585529646 90-91 0.021401838954554088 92-93 0.019867629317682236 94-95 0.023984553669891127 96-97 0.02490970520971335 98-99 0.02787789973330965 100 0.02989781392858817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6485424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.47107125872921 #Duplication Level Percentage of deduplicated Percentage of total 1 80.08635360546639 55.6368477817714 2 12.924643934280617 17.95777719504222 3 3.797829476564841 7.915178465848149 4 1.422046692636816 3.9516442846964974 5 0.6691049624478855 2.3241719262893192 6 0.3354861988955245 1.398395137787471 7 0.19530476621657197 0.9497621931700645 8 0.11747656871144133 0.652897846094683 9 0.07817419387389415 0.4887760493886312 >10 0.3357394966634093 4.011023785848092 >50 0.021429508236438622 1.037283641327469 >100 0.014770287223536682 1.921698174130098 >500 0.001210068317934788 0.538965752050293 >1k 3.495703776067448E-4 0.37395207902094657 >5k 2.6890029039517476E-5 0.18583651121111375 >10k+ 5.378005807903495E-5 0.6557891763234971 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19548 0.3014143716740802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15557 0.23987637508357204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9948 0.15339012530252455 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.709596165185191E-6 0.0 12-13 0.0 0.0 0.0 3.0838384660740765E-5 7.709596165185191E-6 14-15 0.0 0.0 0.0 3.854798082592595E-5 2.3128788495555574E-5 16-17 0.0 0.0 0.0 4.625757699111114E-5 3.0838384660740765E-5 18-19 0.0 0.0 0.0 7.70959616518519E-5 3.854798082592595E-5 20-21 0.0 0.0 0.0 1.3106313480814823E-4 4.625757699111114E-5 22-23 0.0 0.0 0.0 4.0860859675481513E-4 4.625757699111114E-5 24-25 0.0 0.0 0.0 9.945379053088897E-4 4.625757699111114E-5 26-27 0.0 0.0 0.0 0.0019505278297918534 4.625757699111114E-5 28-29 0.0 0.0 0.0 0.0056742627775763 5.3967173156296336E-5 30-31 0.0 0.0 0.0 0.02006807881797705 6.167676932148153E-5 32-33 0.0 0.0 0.0 0.05301118323181337 6.167676932148153E-5 34-35 0.0 0.0 0.0 0.10239114666982452 7.70959616518519E-5 36-37 0.0 0.0 0.0 0.18482985846414976 7.70959616518519E-5 38-39 1.5419192330370383E-5 0.0 0.0 0.3038968616392699 7.70959616518519E-5 40-41 2.3128788495555574E-5 0.0 0.0 0.42655653662736626 7.70959616518519E-5 42-43 3.0838384660740765E-5 0.0 0.0 0.5450453200900974 7.70959616518519E-5 44-45 3.0838384660740765E-5 0.0 0.0 0.6838334702557612 7.70959616518519E-5 46-47 3.0838384660740765E-5 0.0 0.0 0.850638292885708 7.70959616518519E-5 48-49 3.0838384660740765E-5 0.0 0.0 1.0365397852168186 7.70959616518519E-5 50-51 3.0838384660740765E-5 0.0 0.0 1.2261650124957133 7.70959616518519E-5 52-53 3.0838384660740765E-5 0.0 0.0 1.405906537490841 7.70959616518519E-5 54-55 3.0838384660740765E-5 0.0 0.0 1.6004736159116195 7.70959616518519E-5 56-57 3.0838384660740765E-5 0.0 0.0 1.8239285511633472 8.48055578170371E-5 58-59 3.0838384660740765E-5 0.0 0.0 2.0811438080224205 9.251515398222228E-5 60-61 3.0838384660740765E-5 0.0 0.0 2.341211615462613 9.251515398222228E-5 62-63 3.0838384660740765E-5 0.0 0.0 2.603669397714012 1.2335353864296306E-4 64-65 3.0838384660740765E-5 0.0 0.0 2.869596498239745 1.2335353864296306E-4 66-67 4.625757699111114E-5 0.0 0.0 3.1620291903813844 1.2335353864296306E-4 68-69 4.625757699111114E-5 0.0 0.0 3.4868344768206363 1.2335353864296306E-4 70-71 4.625757699111114E-5 0.0 0.0 3.8400264963401005 1.464823271385186E-4 72-73 4.625757699111114E-5 0.0 0.0 4.191622629453372 2.1586869262518534E-4 74-75 4.625757699111114E-5 0.0 0.0 4.527799570236271 2.3899748112074092E-4 76-77 4.625757699111114E-5 0.0 0.0 4.903858251981674 2.544166734511113E-4 78-79 4.625757699111114E-5 0.0 0.0 5.307625222344754 2.6212626961629647E-4 80-81 4.625757699111114E-5 0.0 0.0 5.735739097397487 2.6212626961629647E-4 82-83 4.625757699111114E-5 0.0 0.0 6.168540406918654 2.6212626961629647E-4 84-85 4.625757699111114E-5 0.0 0.0 6.5861306832059086 2.6983586578148167E-4 86-87 4.625757699111114E-5 0.0 0.0 7.02789054347102 2.7754546194666687E-4 88 4.625757699111114E-5 0.0 0.0 7.381321560471605 2.7754546194666687E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12420 0.0 44.44811 1 TACCTGG 4070 0.0 38.274582 2 GTATCAA 21510 0.0 37.785816 1 GTACTAG 6205 0.0 35.571968 1 GCATCGT 5795 0.0 35.286057 8 CTGTGCG 7830 0.0 32.95828 9 CAACGCA 24100 0.0 32.204067 5 GGGCACC 7035 0.0 32.001602 94 ATCAACG 24450 0.0 31.896883 3 ACCTGGG 4820 0.0 31.59939 3 TAGTACT 8175 0.0 31.339067 4 TAGGCAT 7710 0.0 30.607653 5 TATCAAC 26815 0.0 30.117323 2 GTGGTAT 4490 0.0 29.872763 1 ACTGTGC 9370 0.0 29.549013 8 AACGCAG 27620 0.0 28.96787 6 GTACATG 31210 0.0 28.620638 1 GTACCTG 5475 0.0 28.538479 1 TAACGCC 2835 0.0 28.188581 4 CTAGTAC 9540 0.0 27.84074 3 >>END_MODULE