FastQCFastQC Report
Mon 6 Jun 2016
ERR1211219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6485424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186330.28730581069179134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161360.24880408744285648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100460.15490120615090086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132150.044.327681
TACCTGG41850.037.5045662
GTACTAG61250.037.380661
GTATCAA221500.036.252521
GGGCACC70000.033.11437294
TAGGCAT74700.032.8331645
ACCTGGG48000.032.0174373
CAACGCA248250.030.6422335
ATCAACG250700.030.3884623
CTGTGCG84650.029.973199
GCATCGT71900.029.79888
CTAGGCA84550.029.5162354
GTGGTAT47600.029.4329221
TAGTACT92550.029.1484534
CTAACGC25850.028.3624733
TATCAAC282350.028.2607082
TAACGCC26400.028.1276364
GTACATG320700.027.9120731
ACTGTGC100450.027.8310648
TCTAACG27600.027.5828462
Produced by FastQC (version 0.11.5)