##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6485424 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16794707639778 33.0 31.0 34.0 30.0 34.0 2 32.306120154981386 34.0 31.0 34.0 31.0 34.0 3 32.38505454693479 34.0 31.0 34.0 30.0 34.0 4 35.99547400447527 37.0 35.0 37.0 35.0 37.0 5 35.88708402102931 37.0 35.0 37.0 35.0 37.0 6 35.91500123970307 37.0 35.0 37.0 35.0 37.0 7 35.850436455658105 37.0 35.0 37.0 35.0 37.0 8 35.83996435699501 37.0 35.0 37.0 35.0 37.0 9 37.44598641507479 39.0 37.0 39.0 34.0 39.0 10-11 37.42337409242634 39.0 37.0 39.0 34.0 39.0 12-13 37.31794081620569 39.0 37.0 39.0 34.0 39.0 14-15 38.53678001623332 40.0 38.0 41.0 34.0 41.0 16-17 38.49798347802704 40.0 38.0 41.0 34.0 41.0 18-19 38.52344079585236 40.0 38.0 41.0 34.0 41.0 20-21 38.48635254996435 40.0 38.0 41.0 34.0 41.0 22-23 38.35347133818853 40.0 38.0 41.0 34.0 41.0 24-25 38.286290071397026 40.0 38.0 41.0 34.0 41.0 26-27 38.14816517778946 40.0 38.0 41.0 33.5 41.0 28-29 38.12937465615201 40.0 37.5 41.0 33.5 41.0 30-31 38.14166406082316 40.0 37.5 41.0 33.5 41.0 32-33 38.014069935905496 40.0 37.0 41.0 33.0 41.0 34-35 37.87825267862209 40.0 37.0 41.0 33.0 41.0 36-37 37.739935507686155 40.0 37.0 41.0 33.0 41.0 38-39 37.646747614342566 40.0 37.0 41.0 33.0 41.0 40-41 37.54124965461009 40.0 36.0 41.0 32.5 41.0 42-43 37.39510870838977 40.0 36.0 41.0 32.0 41.0 44-45 37.23460964155929 39.5 36.0 41.0 31.5 41.0 46-47 36.97954728942934 39.0 36.0 41.0 31.0 41.0 48-49 36.72098539740809 39.0 35.0 41.0 30.5 41.0 50-51 36.51106673981532 39.0 35.0 41.0 30.0 41.0 52-53 36.29322377380415 39.0 35.0 40.0 30.0 41.0 54-55 36.04546248633859 38.0 35.0 40.0 29.0 41.0 56-57 35.77048108188454 38.0 35.0 40.0 29.0 41.0 58-59 35.478968221661376 37.5 34.0 40.0 28.0 41.0 60-61 35.213607853549746 37.0 34.0 40.0 28.0 41.0 62-63 34.899988728570406 36.5 34.0 39.5 27.5 41.0 64-65 34.52784097076768 36.0 33.5 39.0 27.0 41.0 66-67 34.23082207115526 35.5 33.0 39.0 26.0 40.0 68-69 33.91315556238112 35.0 33.0 38.5 26.0 40.0 70-71 33.62216895919218 35.0 33.0 38.0 26.0 40.0 72-73 33.30612331591581 35.0 32.5 37.0 26.0 40.0 74-75 32.98257137852514 35.0 32.0 37.0 25.0 39.0 76-77 32.003109434325346 34.0 30.5 35.5 24.5 38.0 78-79 32.50026112402212 35.0 32.0 36.0 25.0 38.0 80-81 32.4375297590412 35.0 32.0 35.5 25.0 37.5 82-83 32.197312311423275 35.0 32.0 35.0 25.0 37.0 84-85 31.962126377550643 34.0 32.0 35.0 25.0 36.5 86-87 31.734798757953218 34.0 31.0 35.0 24.0 36.0 88-89 31.526678209474042 34.0 31.0 35.0 24.0 36.0 90-91 31.31886427163436 34.0 31.0 35.0 23.5 35.5 92-93 31.135981086201923 34.0 31.0 35.0 23.0 35.0 94-95 30.946200973136065 34.0 31.0 35.0 22.5 35.0 96-97 30.78314023878778 34.0 30.0 35.0 20.0 35.0 98-99 30.61333715420919 34.0 30.0 35.0 20.0 35.0 100 28.792345573704974 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3415917623097613 1101 2 -0.31360316083957684 1101 3 -0.34842286470658124 1101 4 -0.14837865249268134 1101 5 -0.26728601203964786 1101 6 -0.28824674955652085 1101 7 -0.30234218590861417 1101 8 -0.32231482841032744 1101 9 -0.4710273053135623 1101 10-11 -0.45314416560876225 1101 12-13 -0.5187205596352271 1101 14-15 -0.7070969934585847 1101 16-17 -0.6752505483848026 1101 18-19 -0.6906607846030468 1101 20-21 -0.6992621278159916 1101 22-23 -0.7048787800378662 1101 24-25 -0.7010206570090389 1101 26-27 -0.7865659479738056 1101 28-29 -0.8707450460729049 1101 30-31 -0.7339709506072509 1101 32-33 -0.6616317805704597 1101 34-35 -0.774034815073172 1101 36-37 -0.6983595547933916 1101 38-39 -0.7358038710240109 1101 40-41 -0.7281496951605178 1101 42-43 -0.7598153416269895 1101 44-45 -0.7495690464915867 1101 46-47 -0.7707278839910146 1101 48-49 -0.743979071090898 1101 50-51 -0.8645369970324452 1101 52-53 -0.7842308755602048 1101 54-55 -0.7677668147098444 1101 56-57 -0.7584689724033353 1101 58-59 -0.8510666343907403 1101 60-61 -0.8560821881311966 1101 62-63 -0.8969047301946844 1101 64-65 -0.7942959292224074 1101 66-67 -0.8816315340210608 1101 68-69 -0.9723054270437146 1101 70-71 -1.1011246012867488 1101 72-73 -1.0881846858933812 1101 74-75 -1.0727827601397308 1101 76-77 -0.9596570546279928 1101 78-79 -0.9005144337927398 1101 80-81 -0.8267965750232875 1101 82-83 -0.8429918705830985 1101 84-85 -0.8194628677117848 1101 86-87 -0.8901957629507464 1101 88-89 -0.9204379424099862 1101 90-91 -0.9680351788798411 1101 92-93 -1.0741264441344889 1101 94-95 -1.0190084876675307 1101 96-97 -1.050367159103999 1101 98-99 -1.2114456040784845 1101 100 -0.9387663565641056 1102 1 -0.4043467809544765 1102 2 -0.34009450237052974 1102 3 -0.3765225304882094 1102 4 -0.24775912685397827 1102 5 -0.27916117451876943 1102 6 -0.35051858798580326 1102 7 -0.34934437931494955 1102 8 -0.41937663081721155 1102 9 -0.4909590957591021 1102 10-11 -0.5308890933622195 1102 12-13 -0.5172000291488246 1102 14-15 -0.6515214289523144 1102 16-17 -0.5849752399002526 1102 18-19 -0.5989527843266416 1102 20-21 -0.6928003450166855 1102 22-23 -0.7210349443867088 1102 24-25 -0.7548054803337507 1102 26-27 -0.7579319263894675 1102 28-29 -0.7287304999850832 1102 30-31 -0.688701718044264 1102 32-33 -0.7188206565883206 1102 34-35 -0.6832526031861121 1102 36-37 -0.7939262572515204 1102 38-39 -0.8269933309557658 1102 40-41 -0.7466762911446736 1102 42-43 -0.6618575396054354 1102 44-45 -0.7182649083140831 1102 46-47 -0.7768344929384483 1102 48-49 -0.6949429738355448 1102 50-51 -0.6985498558984418 1102 52-53 -0.8385288475961445 1102 54-55 -0.7306750276322802 1102 56-57 -0.8065188051144219 1102 58-59 -0.8238479615107508 1102 60-61 -0.9503151802010947 1102 62-63 -0.901175577821455 1102 64-65 -0.8936090081658961 1102 66-67 -1.014355666693703 1102 68-69 -0.933762976062873 1102 70-71 -1.0072938700006375 1102 72-73 -0.9330716606325851 1102 74-75 -1.1049835718860876 1102 76-77 -0.8982194366803924 1102 78-79 -0.694958723976562 1102 80-81 -0.7341865095445996 1102 82-83 -0.6349517859452405 1102 84-85 -0.7416793448490537 1102 86-87 -0.7619323083317227 1102 88-89 -0.8693790390844001 1102 90-91 -0.8199682996210704 1102 92-93 -0.9753997053259695 1102 94-95 -0.9269303068262111 1102 96-97 -1.070728527942677 1102 98-99 -1.1226081992980994 1102 100 -1.1700069543416944 1103 1 -0.33757895760418677 1103 2 -0.3142020479762522 1103 3 -0.36953459287049384 1103 4 -0.25273644633625025 1103 5 -0.22957467459596614 1103 6 -0.30960875804657206 1103 7 -0.35840781976455105 1103 8 -0.395676125656685 1103 9 -0.5081398135960882 1103 10-11 -0.519966360308068 1103 12-13 -0.57626438086794 1103 14-15 -0.6709505312901385 1103 16-17 -0.6939627421849153 1103 18-19 -0.6629295708315937 1103 20-21 -0.6838618436547748 1103 22-23 -0.7173337482762676 1103 24-25 -0.8335901657459246 1103 26-27 -0.8179054857314867 1103 28-29 -0.8491060595290776 1103 30-31 -0.7517649136045961 1103 32-33 -0.7216318942024813 1103 34-35 -0.7936555867517114 1103 36-37 -0.8477458636073081 1103 38-39 -0.7875167607042997 1103 40-41 -0.8026951859143239 1103 42-43 -0.7897813233302742 1103 44-45 -0.7712618488132108 1103 46-47 -0.8518018097959157 1103 48-49 -0.8272080339917309 1103 50-51 -0.8398785583677366 1103 52-53 -0.8774942562091042 1103 54-55 -0.9362982930120083 1103 56-57 -0.8734102121831882 1103 58-59 -0.8099421040721779 1103 60-61 -0.8358118587102297 1103 62-63 -0.8493757599715437 1103 64-65 -0.8940481766290063 1103 66-67 -0.9718431991713743 1103 68-69 -0.8736939929606393 1103 70-71 -0.9656713321369494 1103 72-73 -0.969777538995757 1103 74-75 -1.1427641859421627 1103 76-77 -1.0844537417211733 1103 78-79 -0.9151854293756436 1103 80-81 -0.7599751340057601 1103 82-83 -0.7017244972759933 1103 84-85 -0.8423702717018529 1103 86-87 -0.8068750537872127 1103 88-89 -0.7852592888114671 1103 90-91 -0.8839385688187349 1103 92-93 -1.0151774991759304 1103 94-95 -1.0257760282630066 1103 96-97 -1.0783664371928054 1103 98-99 -1.2103785356182648 1103 100 -1.2133772408073398 1104 1 -0.42170707472595836 1104 2 -0.3794685424327895 1104 3 -0.4175191359796244 1104 4 -0.2928266294223647 1104 5 -0.26434434335371293 1104 6 -0.4083135508885647 1104 7 -0.38083529293674445 1104 8 -0.366122261194171 1104 9 -0.4883682299122185 1104 10-11 -0.5540868439884932 1104 12-13 -0.5267238032750612 1104 14-15 -0.7970492444186661 1104 16-17 -0.6819754702204079 1104 18-19 -0.7648147168284325 1104 20-21 -0.7698331770850828 1104 22-23 -0.7866812791634388 1104 24-25 -0.7834239257222109 1104 26-27 -0.7493277220317722 1104 28-29 -0.8044005847587172 1104 30-31 -0.7568589939136601 1104 32-33 -0.7707583989952767 1104 34-35 -0.7951858719306841 1104 36-37 -0.7694292389654862 1104 38-39 -0.7416063783334081 1104 40-41 -0.7804815861249708 1104 42-43 -0.6359329669809028 1104 44-45 -0.7928124182782028 1104 46-47 -0.8518963000384758 1104 48-49 -0.871953074766985 1104 50-51 -0.7713628556309615 1104 52-53 -0.863814980871048 1104 54-55 -0.8839995994780665 1104 56-57 -0.8121479915403071 1104 58-59 -0.8314874494542792 1104 60-61 -0.8904144013484512 1104 62-63 -0.9245559651392696 1104 64-65 -0.8662914823236036 1104 66-67 -1.0236086936870663 1104 68-69 -0.9012721549467102 1104 70-71 -0.9134766920574222 1104 72-73 -0.9481743861389731 1104 74-75 -1.0378032540890985 1104 76-77 -0.9139158678623929 1104 78-79 -0.7289702540257323 1104 80-81 -0.641132871848832 1104 82-83 -0.7985103103339846 1104 84-85 -0.775996842609274 1104 86-87 -0.7199366655361139 1104 88-89 -0.9544645076874048 1104 90-91 -0.8113344645508356 1104 92-93 -0.8926942510684164 1104 94-95 -0.9722615507592174 1104 96-97 -0.9698326037482161 1104 98-99 -1.037194506764429 1104 100 -1.126732485980689 1105 1 -0.3268943127539323 1105 2 -0.33892189053181454 1105 3 -0.3818375778289784 1105 4 -0.2049814329917652 1105 5 -0.24392996275835088 1105 6 -0.351991838129905 1105 7 -0.36525287448407795 1105 8 -0.3976742011075203 1105 9 -0.49849703567476666 1105 10-11 -0.5086554300526913 1105 12-13 -0.577135622231495 1105 14-15 -0.749702517642568 1105 16-17 -0.6797573985561698 1105 18-19 -0.704803764927334 1105 20-21 -0.7646371713024038 1105 22-23 -0.7004912345304319 1105 24-25 -0.7091100905516114 1105 26-27 -0.7342800269469265 1105 28-29 -0.6474659718911582 1105 30-31 -0.6973855936883382 1105 32-33 -0.786220167024851 1105 34-35 -0.6924021300145924 1105 36-37 -0.6543164841392652 1105 38-39 -0.7337612292336644 1105 40-41 -0.7280555789904852 1105 42-43 -0.7913235978702033 1105 44-45 -0.8104569041224039 1105 46-47 -0.6913344399215049 1105 48-49 -0.8083857276171145 1105 50-51 -0.7852765435217464 1105 52-53 -0.7864411329303493 1105 54-55 -0.7633143564461804 1105 56-57 -0.7331676358130466 1105 58-59 -0.8421937639577379 1105 60-61 -0.748425217144451 1105 62-63 -0.7851785646128775 1105 64-65 -0.7202806462099645 1105 66-67 -0.7603603652459299 1105 68-69 -0.7746154302635659 1105 70-71 -0.8305019285299409 1105 72-73 -0.8232293216922244 1105 74-75 -0.9128841494690647 1105 76-77 -0.853861394650913 1105 78-79 -0.6951892755396898 1105 80-81 -0.6987470491985164 1105 82-83 -0.7740573368414658 1105 84-85 -0.8021527749621526 1105 86-87 -0.6991763944202809 1105 88-89 -0.7755755438259087 1105 90-91 -0.7389031280760427 1105 92-93 -0.8324668614569894 1105 94-95 -0.732426229856511 1105 96-97 -0.8139129493974622 1105 98-99 -0.9095239491156022 1105 100 -1.0542712358200816 1106 1 -0.40407665742091936 1106 2 -0.40474726530827 1106 3 -0.47278858966784654 1106 4 -0.30814032018417237 1106 5 -0.32308637766373494 1106 6 -0.35015439072004284 1106 7 -0.4557169702273711 1106 8 -0.4502094549685438 1106 9 -0.5505474024921213 1106 10-11 -0.565339305991607 1106 12-13 -0.6215297280273262 1106 14-15 -0.7422862342811882 1106 16-17 -0.7134233151030074 1106 18-19 -0.7223320539267775 1106 20-21 -0.7517977817235177 1106 22-23 -0.6959589948260216 1106 24-25 -0.6986977288684884 1106 26-27 -0.7719840245145448 1106 28-29 -0.7623198774765001 1106 30-31 -0.8195359276660596 1106 32-33 -0.806149754414605 1106 34-35 -0.7056366210901857 1106 36-37 -0.7703441962538875 1106 38-39 -0.797597766782836 1106 40-41 -0.8428371150577405 1106 42-43 -0.7001179400167672 1106 44-45 -0.8931013860748749 1106 46-47 -0.9197705709895772 1106 48-49 -0.8898147370229808 1106 50-51 -0.9631264237217323 1106 52-53 -0.957550164513755 1106 54-55 -0.9613497382079288 1106 56-57 -1.0849393912583025 1106 58-59 -0.9961754415626345 1106 60-61 -0.9714673391630058 1106 62-63 -1.0220253412934213 1106 64-65 -0.9575071937727913 1106 66-67 -0.9769666868755138 1106 68-69 -0.9668347319974728 1106 70-71 -0.9594085544637707 1106 72-73 -1.0211284328914019 1106 74-75 -1.0493612103467918 1106 76-77 -1.0955162916422907 1106 78-79 -0.820585782577556 1106 80-81 -0.8042273267716951 1106 82-83 -0.8765836811919208 1106 84-85 -0.7915247842417941 1106 86-87 -0.8370674226657293 1106 88-89 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0.43709889309815253 2310 9 0.6324314534913782 2310 10-11 0.6665054727233155 2310 12-13 0.6807996890030239 2310 14-15 0.8977779222098476 2310 16-17 0.655578455502372 2310 18-19 0.680738645551962 2310 20-21 0.8191717503920515 2310 22-23 0.8815696750316135 2310 24-25 0.869322547599154 2310 26-27 0.9407166736854293 2310 28-29 0.9824425909560546 2310 30-31 0.7467390787956418 2310 32-33 0.8652639218044627 2310 34-35 0.9768128275395185 2310 36-37 0.892071001725327 2310 38-39 0.915118442186774 2310 40-41 0.9629020581278454 2310 42-43 1.0369985713444407 2310 44-45 1.0944870822297759 2310 46-47 0.8011210136204809 2310 48-49 0.9220100972871208 2310 50-51 1.0639231024236935 2310 52-53 1.1196509062250684 2310 54-55 1.1665150465511473 2310 56-57 1.1579127402792153 2310 58-59 1.1360460862326889 2310 60-61 1.1464874769219975 2310 62-63 0.5162065749716547 2310 64-65 0.7187594243121538 2310 66-67 1.0821779862562693 2310 68-69 1.2522421262101062 2310 70-71 1.2260920969549076 2310 72-73 1.2398742375404979 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1.0226958140315716 2311 34-35 1.0263771990861912 2311 36-37 1.091818395507559 2311 38-39 1.0866085416661164 2311 40-41 1.0562151826997521 2311 42-43 1.0323672053469863 2311 44-45 1.024960089145587 2311 46-47 0.7650564692343664 2311 48-49 1.0436156438597024 2311 50-51 1.1102143069521802 2311 52-53 1.0342408296393089 2311 54-55 1.0323380412591163 2311 56-57 1.1929958511701386 2311 58-59 1.1913615090182574 2311 60-61 1.2472904782728165 2311 62-63 1.2004174461364059 2311 64-65 1.2398420100966447 2311 66-67 1.2458015784753087 2311 68-69 1.250115986976482 2311 70-71 1.2331225506078454 2311 72-73 1.2455232807530905 2311 74-75 1.25687037915894 2311 76-77 1.2231935341668105 2311 78-79 0.9593972202669576 2311 80-81 0.9553545425775738 2311 82-83 1.010908944332357 2311 84-85 1.0868057059542764 2311 86-87 1.1051911250900837 2311 88-89 1.159880781815744 2311 90-91 1.1408457833860446 2311 92-93 1.2583606851717342 2311 94-95 1.3004196378967983 2311 96-97 1.261750929145471 2311 98-99 1.3516584016667537 2311 100 1.362236076536771 2312 1 0.265886290236935 2312 2 0.3467612153693409 2312 3 0.419480229015079 2312 4 0.18698243498848655 2312 5 0.3230038387582965 2312 6 0.4018458779427263 2312 7 0.2616858820683561 2312 8 0.37024677994782706 2312 9 0.5987540117975527 2312 10-11 0.5963189614173814 2312 12-13 0.6455305715628015 2312 14-15 0.8279207889382008 2312 16-17 0.8267003110081745 2312 18-19 0.8186755183140164 2312 20-21 0.8072917072602976 2312 22-23 0.8987588117662213 2312 24-25 0.8656758596950596 2312 26-27 0.931255268003035 2312 28-29 0.9351085517428075 2312 30-31 0.9255363490453661 2312 32-33 0.7756134200141105 2312 34-35 0.8785487517444395 2312 36-37 0.9634588530992332 2312 38-39 0.7981191020561411 2312 40-41 0.8838545637732906 2312 42-43 0.9369676427841256 2312 44-45 0.7022338754680391 2312 46-47 0.7197125879151258 2312 48-49 0.8122801174730299 2312 50-51 0.7248504524159785 2312 52-53 0.86809026595121 2312 54-55 0.676443782391047 2312 56-57 0.7507315728354556 2312 58-59 0.8934748473869334 2312 60-61 0.9285138535658817 2312 62-63 0.9335915607796323 2312 64-65 0.9091250045956158 2312 66-67 0.9352742078136203 2312 68-69 1.0315538068769499 2312 70-71 0.901137657912173 2312 72-73 0.927974241581957 2312 74-75 0.9058751702243697 2312 76-77 0.8985725352264211 2312 78-79 0.8634534664907036 2312 80-81 0.7941348547234739 2312 82-83 0.8293972964400425 2312 84-85 0.8299666227063796 2312 86-87 0.9029713864694493 2312 88-89 0.8942069658923941 2312 90-91 0.9214378493439561 2312 92-93 0.9503635088050544 2312 94-95 1.0153984821223219 2312 96-97 0.6654556030253964 2312 98-99 0.45233380254811806 2312 100 0.6462580169745991 2313 1 0.017621193073004804 2313 2 0.1851207130250998 2313 3 0.2711046232207721 2313 4 0.18123708495124902 2313 5 0.3060064504140172 2313 6 0.23347518859443994 2313 7 0.24875210490895228 2313 8 0.3688804277226865 2313 9 0.6013153056654517 2313 10-11 0.3671347126790252 2313 12-13 0.3852401920353117 2313 14-15 0.48628816216015025 2313 16-17 0.7655823269000379 2313 18-19 0.7199438647783083 2313 20-21 0.6792020540490071 2313 22-23 0.6586690282026169 2313 24-25 0.5957258101130449 2313 26-27 0.7591577761677897 2313 28-29 0.5606866266182706 2313 30-31 0.8231774941707855 2313 32-33 0.8923714016233788 2313 34-35 0.9195566840676364 2313 36-37 0.916994713415967 2313 38-39 0.9022940961027857 2313 40-41 0.9574193492431178 2313 42-43 1.0051224667138428 2313 44-45 0.8300575514331214 2313 46-47 0.8059987242228033 2313 48-49 0.8927051701348461 2313 50-51 0.8400296044533704 2313 52-53 0.8557843621152657 2313 54-55 0.8169434648188414 2313 56-57 0.5491673296612305 2313 58-59 0.6077055212987474 2313 60-61 0.48488418294854085 2313 62-63 0.8382233764444393 2313 64-65 0.9465311291103546 2313 66-67 0.904911828527915 2313 68-69 1.0165350388809031 2313 70-71 0.9979298869407955 2313 72-73 0.9921540340363677 2313 74-75 1.100765915367674 2313 76-77 1.0303518530990488 2313 78-79 0.8680316921115647 2313 80-81 0.7832200503241538 2313 82-83 0.8728205651085403 2313 84-85 0.9054023483897851 2313 86-87 0.8728669819811579 2313 88-89 0.8809067344064658 2313 90-91 0.8068012140108856 2313 92-93 0.924909998295746 2313 94-95 1.0814180469105459 2313 96-97 1.1808598067143024 2313 98-99 1.211543911016932 2313 100 1.2727057819978 2314 1 -0.3416528874192757 2314 2 0.05365094609527432 2314 3 0.2834492988519628 2314 4 0.23372552680018543 2314 5 0.3259849124236993 2314 6 0.3828658033436625 2314 7 0.4059036712671755 2314 8 0.4457699807481532 2314 9 0.4804509435359492 2314 10-11 0.5480989779584036 2314 12-13 0.47051935695230185 2314 14-15 0.7785480941800387 2314 16-17 0.8329156131008162 2314 18-19 0.8453605154334767 2314 20-21 0.8299228147923188 2314 22-23 0.8513571863228222 2314 24-25 0.7422934279074482 2314 26-27 0.7599058814075121 2314 28-29 0.7853413169151651 2314 30-31 0.8968501673590197 2314 32-33 0.9116326891345921 2314 34-35 0.8816306635823778 2314 36-37 0.8773853092658328 2314 38-39 0.6775247187877582 2314 40-41 0.6834666924413924 2314 42-43 0.8463069373567933 2314 44-45 0.8579231207717513 2314 46-47 0.7869623672465025 2314 48-49 0.8946789784002007 2314 50-51 0.8650422225077747 2314 52-53 0.8785283451758801 2314 54-55 0.8358442743172176 2314 56-57 0.7919392341286908 2314 58-59 0.9268084463936219 2314 60-61 0.9781358945405785 2314 62-63 0.8824493385336467 2314 64-65 0.882927703050008 2314 66-67 0.6186681987463487 2314 68-69 0.6128742441564654 2314 70-71 0.7303166502252338 2314 72-73 0.9730558155775526 2314 74-75 1.00672066559639 2314 76-77 0.8108029444478078 2314 78-79 0.6924082506675902 2314 80-81 0.8511674168272307 2314 82-83 0.8945139373010775 2314 84-85 0.8414282085415934 2314 86-87 0.9450649422998474 2314 88-89 0.914938054918089 2314 90-91 0.9975855759047327 2314 92-93 0.9415161572170021 2314 94-95 0.8953250708565683 2314 96-97 0.9079906608949386 2314 98-99 1.066311288177122 2314 100 1.0844480677898396 2315 1 0.38930075986678503 2315 2 0.4201453746091346 2315 3 0.4202112581062778 2315 4 0.3117021987906696 2315 5 0.3572386752005059 2315 6 0.39831401194555127 2315 7 0.36846610482542275 2315 8 0.23307934371746342 2315 9 0.44506831797318824 2315 10-11 0.5192541913684323 2315 12-13 0.33768497026976263 2315 14-15 0.5239368295465496 2315 16-17 0.6788178602849371 2315 18-19 0.7130758773729795 2315 20-21 0.4730959260724177 2315 22-23 0.7793561952653008 2315 24-25 0.8349003142504898 2315 26-27 0.756759319432625 2315 28-29 0.7050684949685504 2315 30-31 0.7487298239889597 2315 32-33 0.7488848546350653 2315 34-35 0.7890036710473396 2315 36-37 0.7977356977376857 2315 38-39 0.8710222099654885 2315 40-41 0.8584263235128233 2315 42-43 0.9180068899629248 2315 44-45 0.9295920090666385 2315 46-47 0.8777503851692856 2315 48-49 0.7686657437316811 2315 50-51 0.8713977359876282 2315 52-53 0.9234535357917153 2315 54-55 0.729320706990201 2315 56-57 0.7336208247101368 2315 58-59 0.7334287060775679 2315 60-61 0.7592387548836541 2315 62-63 0.8882025142031225 2315 64-65 0.9823855827139383 2315 66-67 0.924146368020331 2315 68-69 0.8863305875964471 2315 70-71 0.8069572796178619 2315 72-73 0.8945883926962921 2315 74-75 0.24278205206721282 2315 76-77 0.30878718862914667 2315 78-79 0.6391782243511486 2315 80-81 0.7176255628778208 2315 82-83 0.6820726953235052 2315 84-85 0.732385643553755 2315 86-87 0.7550312930465388 2315 88-89 0.8026432929904033 2315 90-91 0.8690387164429971 2315 92-93 0.8687081626428359 2315 94-95 0.9015058088571983 2315 96-97 1.0576238932961672 2315 98-99 1.0589377621583083 2315 100 1.0280775782136864 2316 1 0.26093946251503297 2316 2 0.056245620471202074 2316 3 0.22187835321658156 2316 4 -7.006815023515856E-4 2316 5 0.2696252467125504 2316 6 0.38361326891435255 2316 7 0.43178409621187797 2316 8 0.43593237254342654 2316 9 0.6187390999674562 2316 10-11 0.6534324617894924 2316 12-13 0.3828593119237951 2316 14-15 0.5401844853228894 2316 16-17 0.7579981045135114 2316 18-19 0.7241003384502278 2316 20-21 0.7577269876772021 2316 22-23 0.813789908840171 2316 24-25 0.4924335181955968 2316 26-27 0.7167438732107669 2316 28-29 0.9051000457844154 2316 30-31 0.5641186444374 2316 32-33 0.7017499907021261 2316 34-35 0.7857074255747918 2316 36-37 0.8473251902754342 2316 38-39 0.7544889999994879 2316 40-41 0.7612537373433028 2316 42-43 0.7247830117609908 2316 44-45 0.5970567501627784 2316 46-47 0.779821554542913 2316 48-49 0.8199802890700951 2316 50-51 0.8220683200775909 2316 52-53 0.936678602466614 2316 54-55 0.7576321159641708 2316 56-57 0.9294456000782745 2316 58-59 0.7531522198842708 2316 60-61 0.9026134565874102 2316 62-63 0.9373610544023094 2316 64-65 0.9182013673102816 2316 66-67 0.9376914585275244 2316 68-69 0.9482834384656016 2316 70-71 0.9118914581107873 2316 72-73 0.9251218403568942 2316 74-75 0.9054417236717143 2316 76-77 0.8008326657827141 2316 78-79 0.5545792371185954 2316 80-81 0.5660034412598662 2316 82-83 0.4632240376696828 2316 84-85 0.5879412834600544 2316 86-87 0.6244956333602794 2316 88-89 0.6423944348668513 2316 90-91 0.48864327169756905 2316 92-93 0.3313526445680992 2316 94-95 0.40565852710952655 2316 96-97 0.6703465290988362 2316 98-99 0.8431128716077652 2316 100 0.4295674364032074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 21.0 13 134.0 14 501.0 15 1551.0 16 3682.0 17 7983.0 18 13557.0 19 20051.0 20 29104.0 21 41463.0 22 50939.0 23 51816.0 24 51275.0 25 54574.0 26 64203.0 27 78575.0 28 98035.0 29 120277.0 30 149090.0 31 185935.0 32 237019.0 33 314420.0 34 454022.0 35 632217.0 36 996072.0 37 1453812.0 38 1175418.0 39 199652.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.334047607992325 21.20032079884404 13.481326147496816 22.984305445666816 2 15.424265244647072 20.17789430575395 41.25705582241038 23.1407846271886 3 18.98898206192841 26.535335237911973 28.987557328557084 25.488125371602532 4 12.295505126996092 16.454958954879135 39.32115021044291 31.928385707681866 5 12.802092816136618 38.6633009653648 34.6164414231051 13.918164795393487 6 30.125786687192697 37.92544635477958 17.90068004805854 14.048086909969188 7 27.481728543962436 32.14707706816044 22.60141989940773 17.769774488469388 8 26.508845065488394 35.77221782261268 19.45436412484365 18.26457298705528 9 26.550064266509786 15.358130619464387 21.41662355246144 36.675181561564386 10-11 25.990523478402594 26.40238727115605 26.962175712772535 20.64491353766882 12-13 25.846425129093355 23.94267883528954 27.890763640243666 22.32013239537344 14-15 23.180275069436554 25.232147755495138 25.673453225906677 25.91412394916163 16-17 21.671788936575442 27.991624579864393 26.908623762336543 23.427962721223622 18-19 21.142765685019207 27.807726989014135 29.365319831054993 21.684187494911665 20-21 22.691576585147427 26.884187335843375 28.944932187913135 21.47930389109606 22-23 22.878627519187646 26.707398005126574 28.58089925963206 21.833075216053725 24-25 22.263957911551966 26.607112293940688 29.40326641882755 21.725663375679797 26-27 22.20847250838905 26.92516060142898 28.457374366604927 22.40899252357704 28-29 21.541011045164016 27.34693641542738 28.992782654316397 22.11926988509221 30-31 22.463893148545203 26.930071477418426 28.956210733100345 21.64982464093603 32-33 22.644765274593226 27.11805112185686 28.22125559879761 22.015928004752308 34-35 22.606421741722198 27.046148412455434 28.791016933860085 21.55641291196228 36-37 22.254012628331807 26.678594495589724 28.542513134429075 22.524879741649396 38-39 22.43484696794078 26.843131719392606 29.04097010102353 21.681051211643087 40-41 22.141956539806642 26.077866115515665 29.54494114955013 22.235236195127563 42-43 22.18314740091661 26.18742098623975 28.878757446895122 22.750674165948517 44-45 22.187890315649728 26.996598396341685 28.751195788941587 22.064315499067003 46-47 22.272174677300182 26.814415855460833 28.066328751840796 22.847080715398185 48-49 22.909185557815736 26.834601241221584 28.117401958667887 22.138811242294793 50-51 22.54280257497824 26.65069320702606 28.116455387784967 22.690048830210735 52-53 22.127333604482835 27.17638917535212 27.776968612838992 22.919308607326048 54-55 22.037963110174008 27.137759263282934 27.796857528772424 23.027420097770637 56-57 22.901623894670426 26.62607649556018 27.460882882093863 23.011416727675535 58-59 21.94112675287482 27.099236111507334 28.136878171528178 22.82275896408967 60-61 22.14977512736666 27.708157240992854 27.49971068364164 22.642356947998845 62-63 22.45894632379959 26.988431312353022 27.2068620495637 23.345760314283684 64-65 21.742260619150468 27.12319575762273 27.494318933328397 23.6402246898984 66-67 22.103174168488877 26.89789084582934 27.524178818110272 23.47475616757151 68-69 21.84888658220298 26.869420346682244 27.74228565402761 23.53940741708717 70-71 21.76902966622651 26.481959533243327 27.862901045496503 23.88610975503366 72-73 22.207671944000644 26.715768585066602 27.878961259254336 23.19759821167842 74-75 22.497307448332506 27.043221575889852 27.349021407376416 23.110449568401222 76-77 22.25473777809997 26.867153268909753 27.096701302597364 23.781407650392907 78-79 22.188439699829637 26.86710871899564 27.160472027624234 23.783979553550484 80-81 21.74563138054049 27.37698262420621 27.513297675497245 23.364088319756053 82-83 22.635583918299503 26.436735422943343 28.38149984976082 22.546180808996336 84-85 22.76470017434119 26.851011791363838 27.256203490310345 23.128084543984627 86-87 21.581104392857466 27.457655987134977 27.66938087268112 23.29185874732644 88-89 22.41050298853767 26.450720469988294 27.5350649300553 23.60371161141874 90-91 22.204180009993323 26.498431350830852 27.549211093728815 23.748177545447014 92-93 22.52079684468103 26.66651447127514 27.36303976564789 23.44964891839594 94-95 21.57454148680711 26.633987570655187 27.945168637536455 23.846302305001245 96-97 21.83843318325513 26.564404704915805 27.582503755296024 24.01465835653304 98-99 21.287980886494243 27.013149210406567 27.447032515337945 24.251837387761245 100 21.783986347836496 26.60728189422535 26.843748496152 24.76498326178616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 867.0 1 618.0 2 541.5 3 750.5 4 629.0 5 536.5 6 823.5 7 1533.5 8 2431.5 9 2907.0 10 2784.5 11 2570.5 12 2807.0 13 3352.5 14 4012.0 15 4878.0 16 5774.5 17 6499.0 18 7248.5 19 8154.0 20 9032.5 21 9917.5 22 11004.5 23 12455.5 24 14146.5 25 16276.0 26 19788.5 27 24099.5 28 29744.0 29 40442.0 30 55657.5 31 70838.0 32 91739.5 33 115538.0 34 135179.0 35 155489.0 36 175360.5 37 202108.0 38 214728.5 39 207837.0 40 196295.0 41 183080.5 42 181462.0 43 189722.0 44 204051.5 45 235851.5 46 265155.5 47 281288.5 48 296265.5 49 296430.0 50 284893.5 51 286374.5 52 284738.5 53 266992.5 54 250081.0 55 218294.0 56 187913.5 57 154748.5 58 122315.0 59 114966.5 60 94708.5 61 64006.0 62 47020.5 63 38971.5 64 27708.0 65 13742.5 66 7829.0 67 5221.5 68 3820.5 69 2881.0 70 2160.5 71 1563.5 72 1152.5 73 947.0 74 741.5 75 517.5 76 336.0 77 175.0 78 101.0 79 72.5 80 52.5 81 37.0 82 26.0 83 13.0 84 5.0 85 4.5 86 6.5 87 7.5 88 3.0 89 0.5 90 1.0 91 2.0 92 2.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04405263248786818 2 0.0 3 0.0 4 0.033814288780502244 5 0.0 6 0.0 7 5.396717315629633E-4 8 0.0 9 0.010932207362232601 10-11 4.240277890851855E-4 12-13 1.5419192330370383E-5 14-15 1.0793434631259267E-4 16-17 2.7754546194666687E-4 18-19 0.0 20-21 3.0838384660740765E-5 22-23 0.0 24-25 5.242525392325929E-4 26-27 5.62800520058519E-4 28-29 1.0022475014740748E-4 30-31 0.0024516515805288906 32-33 0.015357515561048899 34-35 0.025734631999388163 36-37 0.030630225564280765 38-39 0.022195927359568164 40-41 0.001341469732742223 42-43 4.625757699111114E-5 44-45 0.010616113919460008 46-47 0.01768581360293483 48-49 0.0030838384660740767 50-51 0.010932207362232601 52-53 0.010585275534799267 54-55 0.0100995709763926 56-57 4.317373852503707E-4 58-59 0.008896873974623711 60-61 0.004756820833919263 62-63 0.001125601040117038 64-65 0.03494759941678447 66-67 0.05891673389434522 68-69 0.05832309498962596 70-71 0.050706013978423004 72-73 0.045039460797011883 74-75 0.03487050345513262 76-77 0.03132408921914743 78-79 0.01500287413745038 80-81 0.0012335353864296305 82-83 0.037437798978139286 84-85 0.05099126903653485 86-87 0.05648821110231189 88-89 0.06802176696542894 90-91 0.04772240026249633 92-93 0.03900284699967188 94-95 0.01311402307698001 96-97 0.00384708848642741 98-99 0.021933801089951868 100 0.03170185943124151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6485424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.64736365453919 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35860626873985 53.00753133724299 2 13.977641723026165 18.91101225340822 3 4.112365030662074 8.345719581282227 4 1.5866859248401626 4.293404790528053 5 0.7387324495762951 2.498665132994809 6 0.3693351634891824 1.4990730068976845 7 0.21970627932243275 1.0403785402157262 8 0.13898473777331743 0.7521560878865899 9 0.09057386507190127 0.5514374869304464 >10 0.36683123736398976 4.203114636340587 >50 0.02320791167567736 1.097040406647407 >100 0.015580548880656624 2.0148490454552728 >500 0.0013324681266963338 0.5970874394678941 >1k 3.331131614087338E-4 0.3469707627915565 >5k 0.0 0.0 >10k+ 8.32782903325618E-5 0.8415594919106949 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18633 0.28730581069179134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16136 0.24880408744285648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10046 0.15490120615090086 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5419192330370383E-5 0.0 4 0.0 0.0 0.0 1.5419192330370383E-5 0.0 5 0.0 0.0 0.0 1.5419192330370383E-5 0.0 6 0.0 0.0 0.0 1.5419192330370383E-5 0.0 7 0.0 0.0 0.0 1.5419192330370383E-5 0.0 8 0.0 0.0 0.0 1.5419192330370383E-5 1.5419192330370383E-5 9 0.0 0.0 0.0 1.5419192330370383E-5 1.5419192330370383E-5 10-11 0.0 0.0 0.0 1.5419192330370383E-5 1.5419192330370383E-5 12-13 0.0 0.0 0.0 1.5419192330370383E-5 2.3128788495555574E-5 14-15 0.0 0.0 0.0 2.3128788495555574E-5 3.0838384660740765E-5 16-17 0.0 0.0 0.0 3.0838384660740765E-5 4.625757699111114E-5 18-19 0.0 0.0 0.0 6.167676932148153E-5 6.167676932148153E-5 20-21 0.0 0.0 0.0 1.3877273097333344E-4 7.70959616518519E-5 22-23 0.0 0.0 0.0 4.4715657758074103E-4 8.48055578170371E-5 24-25 0.0 0.0 0.0 0.0010716338669607417 9.251515398222228E-5 26-27 0.0 0.0 0.0 0.0020430429837740758 1.0793434631259267E-4 28-29 0.0 0.0 0.0 0.005836164297045189 1.0793434631259267E-4 30-31 0.0 0.0 0.0 0.020445849030071128 1.0793434631259267E-4 32-33 0.0 0.0 0.0 0.05376672365600152 1.2335353864296306E-4 34-35 0.0 0.0 0.0 0.10364010124858453 1.2335353864296306E-4 36-37 0.0 0.0 0.0 0.1865182600243253 1.2335353864296306E-4 38-39 0.0 0.0 0.0 0.30668773545106687 1.3877273097333344E-4 40-41 0.0 0.0 0.0 0.4307042993642359 1.541919233037038E-4 42-43 0.0 0.0 0.0 0.5501953303284411 1.541919233037038E-4 44-45 0.0 0.0 0.0 0.6907181396312716 1.696111156340742E-4 46-47 0.0 0.0 0.0 0.8589415279556125 1.696111156340742E-4 48-49 7.709596165185191E-6 0.0 0.0 1.0457990102112058 1.696111156340742E-4 50-51 1.5419192330370383E-5 0.0 0.0 1.2363262602414276 1.696111156340742E-4 52-53 1.5419192330370383E-5 0.0 0.0 1.416854164045404 1.696111156340742E-4 54-55 1.5419192330370383E-5 7.709596165185191E-6 0.0 1.61193778540925 1.696111156340742E-4 56-57 1.5419192330370383E-5 1.5419192330370383E-5 0.0 1.8359709403733664 1.7732071179925936E-4 58-59 1.5419192330370383E-5 1.5419192330370383E-5 0.0 2.093656482598516 1.8503030796444456E-4 60-61 1.5419192330370383E-5 1.5419192330370383E-5 0.0 2.3542331233856104 1.8503030796444456E-4 62-63 1.5419192330370383E-5 1.5419192330370383E-5 0.0 2.617461865253529 1.8503030796444456E-4 64-65 1.5419192330370383E-5 1.5419192330370383E-5 0.0 2.883843831953007 1.8503030796444456E-4 66-67 1.5419192330370383E-5 1.5419192330370383E-5 0.0 3.1775023498849113 1.8503030796444456E-4 68-69 1.5419192330370383E-5 1.5419192330370383E-5 0.0 3.503101724729177 1.8503030796444456E-4 70-71 1.5419192330370383E-5 1.5419192330370383E-5 0.0 3.857696890750705 2.0044950029481497E-4 72-73 1.5419192330370383E-5 1.5419192330370383E-5 0.0 4.210780975923857 2.0044950029481497E-4 74-75 1.5419192330370383E-5 1.5419192330370383E-5 0.0 4.549355601114129 2.0044950029481497E-4 76-77 1.5419192330370383E-5 1.5419192330370383E-5 0.0 4.927858224843896 2.0044950029481497E-4 78-79 1.5419192330370383E-5 1.5419192330370383E-5 0.0 5.334231038710808 2.0044950029481497E-4 80-81 1.5419192330370383E-5 1.5419192330370383E-5 0.0 5.763971638554395 2.0815909646000017E-4 82-83 1.5419192330370383E-5 1.5419192330370383E-5 0.0 6.198869958232492 2.1586869262518534E-4 84-85 1.5419192330370383E-5 1.5419192330370383E-5 0.0 6.618271989618567 2.1586869262518534E-4 86-87 1.5419192330370383E-5 1.5419192330370383E-5 0.0 7.062290761560077 2.1586869262518534E-4 88 1.5419192330370383E-5 1.5419192330370383E-5 0.0 7.417109505870395 2.3128788495555572E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13215 0.0 44.32768 1 TACCTGG 4185 0.0 37.504566 2 GTACTAG 6125 0.0 37.38066 1 GTATCAA 22150 0.0 36.25252 1 GGGCACC 7000 0.0 33.114372 94 TAGGCAT 7470 0.0 32.833164 5 ACCTGGG 4800 0.0 32.017437 3 CAACGCA 24825 0.0 30.642233 5 ATCAACG 25070 0.0 30.388462 3 CTGTGCG 8465 0.0 29.97319 9 GCATCGT 7190 0.0 29.7988 8 CTAGGCA 8455 0.0 29.516235 4 GTGGTAT 4760 0.0 29.432922 1 TAGTACT 9255 0.0 29.148453 4 CTAACGC 2585 0.0 28.362473 3 TATCAAC 28235 0.0 28.260708 2 TAACGCC 2640 0.0 28.127636 4 GTACATG 32070 0.0 27.912073 1 ACTGTGC 10045 0.0 27.831064 8 TCTAACG 2760 0.0 27.582846 2 >>END_MODULE