FastQCFastQC Report
Mon 6 Jun 2016
ERR1211218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8156244
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188290.23085380967023547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150370.18436182144624413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92450.11334874238681432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125450.044.9052541
GTATCAA215000.039.729381
GTACTAG55500.034.5971871
CAACGCA240350.033.8552065
ATCAACG245850.033.1549763
TATCAAC267800.031.5976942
AACGCAG271100.030.7957866
GCATCGT57100.030.705868
TCAACGC275650.029.87784
TAGGCAT70250.028.5059685
GTACATG315800.027.599581
ACGCAGA296900.027.3442067
GTGGTAT48450.027.2951931
TACATGG321600.026.8093152
CGCAGAG305300.026.497368
CTAGGCA80250.025.7739034
TACCTGG55650.025.6244052
TAGTACT79150.025.4787964
ACATGGG321200.025.4649933
CTGTGCG77450.024.8839289
Produced by FastQC (version 0.11.5)