FastQCFastQC Report
Mon 6 Jun 2016
ERR1211218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8156244
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175330.21496414280887133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152880.18743921834609165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.11434184656564958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127800.045.9495771
GTATCAA216400.038.151991
CAACGCA249550.031.3876635
GTACTAG55000.031.3728031
ATCAACG256350.030.5831453
TATCAAC278500.029.3456132
CTGTGCG82000.028.707779
AACGCAG282050.028.453966
TAGTACT85600.028.1135464
TCAACGC285850.027.6078074
GTACATG319550.026.778271
GTGGTAT48700.026.645881
GGGCACC77650.026.51746694
TACCTGG51050.026.2372262
CTAGTAC92850.026.1714653
TACATGG323850.026.1069492
GTACTGT98900.025.940446
ACGCAGA307150.025.5474117
ACATGGG319900.025.462663
CGCAGAG315400.024.849068
Produced by FastQC (version 0.11.5)