##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211218_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8156244 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14709307372364 33.0 31.0 34.0 30.0 34.0 2 32.29039727109684 34.0 31.0 34.0 30.0 34.0 3 32.36875380383422 34.0 31.0 34.0 30.0 34.0 4 35.98481985090196 37.0 35.0 37.0 35.0 37.0 5 35.86604679801144 37.0 35.0 37.0 35.0 37.0 6 35.88989527532526 37.0 35.0 37.0 35.0 37.0 7 35.82414503538639 37.0 35.0 37.0 35.0 37.0 8 35.80883713140509 37.0 35.0 37.0 35.0 37.0 9 37.41389602861317 39.0 37.0 39.0 34.0 39.0 10-11 37.38869141237069 39.0 37.0 39.0 34.0 39.0 12-13 37.29088947069263 39.0 37.0 39.0 34.0 39.0 14-15 38.52473338217935 40.0 38.0 41.0 34.0 41.0 16-17 38.47198753494868 40.0 38.0 41.0 34.0 41.0 18-19 38.48127269120443 40.0 38.0 41.0 34.0 41.0 20-21 38.43808988549141 40.0 38.0 41.0 34.0 41.0 22-23 38.30983716279209 40.0 38.0 41.0 34.0 41.0 24-25 38.24693480234284 40.0 38.0 41.0 34.0 41.0 26-27 38.11215854994039 40.0 37.5 41.0 33.0 41.0 28-29 38.093108850103064 40.0 37.5 41.0 33.0 41.0 30-31 38.11989342153079 40.0 37.5 41.0 33.5 41.0 32-33 38.00883611377982 40.0 37.0 41.0 33.0 41.0 34-35 37.87704481376477 40.0 37.0 41.0 33.0 41.0 36-37 37.74466059377331 40.0 37.0 41.0 33.0 41.0 38-39 37.6617805941068 40.0 37.0 41.0 33.0 41.0 40-41 37.56295642945454 40.0 36.5 41.0 32.5 41.0 42-43 37.42535816731329 40.0 36.0 41.0 32.0 41.0 44-45 37.29283282844408 39.5 36.0 41.0 31.5 41.0 46-47 37.06895962161015 39.0 36.0 41.0 31.0 41.0 48-49 36.84837444784634 39.0 35.5 41.0 31.0 41.0 50-51 36.64872771094146 39.0 35.0 41.0 30.5 41.0 52-53 36.43710879664708 39.0 35.0 40.0 30.0 41.0 54-55 36.19387428821403 38.0 35.0 40.0 29.5 41.0 56-57 35.929027687254084 38.0 35.0 40.0 29.0 41.0 58-59 35.64525711835006 38.0 34.0 40.0 28.5 41.0 60-61 35.37435895738284 37.0 34.0 40.0 28.0 41.0 62-63 35.06258358626839 37.0 34.0 40.0 28.0 41.0 64-65 34.68138502232155 36.0 34.0 39.0 27.0 41.0 66-67 34.37091319239591 35.5 33.0 39.0 27.0 40.0 68-69 34.03334463755621 35.0 33.0 38.5 26.0 40.0 70-71 33.71248217929724 35.0 33.0 38.0 26.0 40.0 72-73 33.37833474574816 35.0 32.5 37.0 26.0 39.5 74-75 33.05068797598503 35.0 32.0 36.5 26.0 39.0 76-77 32.080304856009704 34.0 30.5 35.5 24.5 38.0 78-79 32.582100903307946 35.0 32.0 36.0 26.0 38.0 80-81 32.51908323242904 35.0 32.0 35.5 26.0 37.0 82-83 32.28200253695206 34.5 32.0 35.0 25.0 37.0 84-85 32.04804938400568 34.0 32.0 35.0 25.0 36.5 86-87 31.826604991219 34.0 31.0 35.0 25.0 36.0 88-89 31.621624794451954 34.0 31.0 35.0 24.5 36.0 90-91 31.4214306854969 34.0 31.0 35.0 24.0 35.0 92-93 31.24926951670401 34.0 31.0 35.0 24.0 35.0 94-95 31.066037014096196 34.0 31.0 35.0 23.0 35.0 96-97 30.901416877670655 34.0 30.0 35.0 23.0 35.0 98-99 30.736571208512153 34.0 30.0 35.0 20.5 35.0 100 28.92584858913 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3287889619518012 1101 2 -0.33151939132409325 1101 3 -0.378664277998908 1101 4 -0.18242719442734057 1101 5 -0.245240188364086 1101 6 -0.3347823391497755 1101 7 -0.354241356522337 1101 8 -0.3291184118299597 1101 9 -0.5157540121257043 1101 10-11 -0.5070522609957493 1101 12-13 -0.47669534785803336 1101 14-15 -0.6685303856085554 1101 16-17 -0.6380297700846356 1101 18-19 -0.6829329792856669 1101 20-21 -0.7039682964957663 1101 22-23 -0.7587471923142814 1101 24-25 -0.7265457465618965 1101 26-27 -0.8578629451149737 1101 28-29 -0.84341606128298 1101 30-31 -0.7016772070733595 1101 32-33 -0.6993130789991469 1101 34-35 -0.7317174311010533 1101 36-37 -0.704404155973485 1101 38-39 -0.8404376905674766 1101 40-41 -0.9106560774311205 1101 42-43 -0.8206594634370177 1101 44-45 -0.8328775239353092 1101 46-47 -0.8364980881976507 1101 48-49 -0.7752264782494152 1101 50-51 -0.8530805078405521 1101 52-53 -0.8536338683290197 1101 54-55 -0.7991059337456647 1101 56-57 -0.8733176835230552 1101 58-59 -0.8162717759155811 1101 60-61 -0.8965874931405082 1101 62-63 -0.9839591508807715 1101 64-65 -1.019734985060353 1101 66-67 -1.003870429033178 1101 68-69 -1.1442936962538894 1101 70-71 -1.1431917589144618 1101 72-73 -1.164981536958372 1101 74-75 -1.1850678556124308 1101 76-77 -1.1049811720293299 1101 78-79 -1.0147222256763158 1101 80-81 -0.9528226174679943 1101 82-83 -0.9213316236892553 1101 84-85 -0.8848703335200447 1101 86-87 -1.0405583543049595 1101 88-89 -1.0301671793593847 1101 90-91 -1.0225604951992864 1101 92-93 -1.1169105583355794 1101 94-95 -1.0945282168627593 1101 96-97 -1.1992368662030337 1101 98-99 -1.328313159930481 1101 100 -1.2057545775152612 1102 1 -0.3705547254341077 1102 2 -0.31596990260367974 1102 3 -0.3301354805761214 1102 4 -0.2179620555802586 1102 5 -0.26464115227415164 1102 6 -0.2875787979185631 1102 7 -0.3442956607882053 1102 8 -0.3575073734097245 1102 9 -0.44689923247279495 1102 10-11 -0.5123823255240083 1102 12-13 -0.5252092560617783 1102 14-15 -0.6063013351313415 1102 16-17 -0.6332991812921236 1102 18-19 -0.6120686356456915 1102 20-21 -0.6599236192269231 1102 22-23 -0.6756332131620368 1102 24-25 -0.7352972343713375 1102 26-27 -0.8782261726419947 1102 28-29 -0.8752358620397729 1102 30-31 -0.7568134981608878 1102 32-33 -0.6944726944532462 1102 34-35 -0.7477586467377009 1102 36-37 -0.7863070889449446 1102 38-39 -0.8871920900068702 1102 40-41 -0.8304847615249642 1102 42-43 -0.8020753329415982 1102 44-45 -0.8027789542793045 1102 46-47 -0.9511364065506953 1102 48-49 -0.8625166564020788 1102 50-51 -0.8999689369505219 1102 52-53 -0.9536196999866817 1102 54-55 -0.8887526934760288 1102 56-57 -0.9356178872018788 1102 58-59 -0.9912847116477295 1102 60-61 -0.9989278594419915 1102 62-63 -0.9487786940798912 1102 64-65 -0.935102661553664 1102 66-67 -1.0388575128803907 1102 68-69 -1.0681008633347844 1102 70-71 -1.1063011956534368 1102 72-73 -1.0279731326038473 1102 74-75 -1.1984180651739642 1102 76-77 -0.9869447565416216 1102 78-79 -0.8470684266293347 1102 80-81 -0.8134945920418701 1102 82-83 -0.8926303355982625 1102 84-85 -0.8980514574433833 1102 86-87 -0.840520432629905 1102 88-89 -0.9839484447169511 1102 90-91 -0.9707177029251639 1102 92-93 -1.008126960363363 1102 94-95 -1.1367610550576224 1102 96-97 -1.2517821038867645 1102 98-99 -1.2267814037092748 1102 100 -1.0881264541389015 1103 1 -0.3579697719069763 1103 2 -0.3510000668053692 1103 3 -0.4043994356158933 1103 4 -0.26056480299341445 1103 5 -0.23366482746288852 1103 6 -0.36531926242574286 1103 7 -0.44640136133065766 1103 8 -0.4130193447606558 1103 9 -0.5259967247157675 1103 10-11 -0.541434318352934 1103 12-13 -0.5522413133746085 1103 14-15 -0.6998123360453192 1103 16-17 -0.7534131630953311 1103 18-19 -0.6997301959127 1103 20-21 -0.6645818933943275 1103 22-23 -0.7387889872879256 1103 24-25 -0.8192102141337543 1103 26-27 -0.7975538866515421 1103 28-29 -0.8420713921776155 1103 30-31 -0.8092022329953821 1103 32-33 -0.7209769176633074 1103 34-35 -0.8531259589285369 1103 36-37 -0.8074946794554805 1103 38-39 -0.8072562449778715 1103 40-41 -0.8191833545438882 1103 42-43 -0.8184762478378715 1103 44-45 -0.8840829012139579 1103 46-47 -0.8638927618850687 1103 48-49 -0.865941139800718 1103 50-51 -0.9534012953144 1103 52-53 -0.9053910489845194 1103 54-55 -0.9863640054337992 1103 56-57 -0.9584162841750654 1103 58-59 -0.9319629659051927 1103 60-61 -1.015085861815649 1103 62-63 -0.888218495672362 1103 64-65 -0.9046228286914868 1103 66-67 -0.8981804786879692 1103 68-69 -0.9581311417442677 1103 70-71 -1.0495533798731387 1103 72-73 -1.022020965600234 1103 74-75 -1.0434286455987625 1103 76-77 -0.9376650039125813 1103 78-79 -0.7899692962410896 1103 80-81 -0.7844274957089681 1103 82-83 -0.8080695688899837 1103 84-85 -0.9293758040734268 1103 86-87 -0.893296295487108 1103 88-89 -0.9418899260304912 1103 90-91 -0.9579144634722496 1103 92-93 -1.025643359579874 1103 94-95 -1.1279128453245555 1103 96-97 -1.0558792417216374 1103 98-99 -1.0217387726612017 1103 100 -1.2870944697526845 1104 1 -0.4190358988642018 1104 2 -0.3863445487653365 1104 3 -0.43579007803499437 1104 4 -0.26925324941401385 1104 5 -0.2711338279571933 1104 6 -0.4189931010870396 1104 7 -0.40592164727464564 1104 8 -0.42646318237671466 1104 9 -0.5670632191637921 1104 10-11 -0.5539490368113107 1104 12-13 -0.640311236632293 1104 14-15 -0.6823514022752946 1104 16-17 -0.6818955375437596 1104 18-19 -0.7972768634790128 1104 20-21 -0.7823230840825914 1104 22-23 -0.7645323202668237 1104 24-25 -0.7740528241498055 1104 26-27 -0.8780593732590631 1104 28-29 -0.9474290465742001 1104 30-31 -0.9091588269099091 1104 32-33 -0.8252483656463383 1104 34-35 -0.9179213644501161 1104 36-37 -0.8673480867729495 1104 38-39 -0.8351387057565134 1104 40-41 -0.9141145117694052 1104 42-43 -0.7642898799994597 1104 44-45 -0.9264550422786186 1104 46-47 -0.9820015939842435 1104 48-49 -1.0651731141510652 1104 50-51 -0.871629170334991 1104 52-53 -0.9248692662685727 1104 54-55 -0.9683087607225787 1104 56-57 -0.9542345702160375 1104 58-59 -0.9969574791470635 1104 60-61 -0.9411562013620838 1104 62-63 -0.999556159171874 1104 64-65 -1.0810830247689083 1104 66-67 -1.0197426700295793 1104 68-69 -1.0091951932821317 1104 70-71 -1.06036242894249 1104 72-73 -1.0564944642421779 1104 74-75 -1.1370327694479805 1104 76-77 -0.9574492627152651 1104 78-79 -0.7671542926158814 1104 80-81 -0.7654630124898922 1104 82-83 -0.7414483064144974 1104 84-85 -0.8021550074678352 1104 86-87 -0.8523588746520048 1104 88-89 -0.8633934393329099 1104 90-91 -0.9044236171559987 1104 92-93 -0.960985461886299 1104 94-95 -0.9420825375148674 1104 96-97 -0.9290281950807291 1104 98-99 -1.0313188361045746 1104 100 -1.0857134272776747 1105 1 -0.41862215150232274 1105 2 -0.4166706973206935 1105 3 -0.4201483279581275 1105 4 -0.24311536197519956 1105 5 -0.33713281703895603 1105 6 -0.4302878881990537 1105 7 -0.376570724029321 1105 8 -0.498675520472716 1105 9 -0.6121423365853431 1105 10-11 -0.5366172219442333 1105 12-13 -0.5914277886901331 1105 14-15 -0.8215507515414018 1105 16-17 -0.7286366195198113 1105 18-19 -0.7845025090437829 1105 20-21 -0.8022681845412976 1105 22-23 -0.7579652842845874 1105 24-25 -0.8074610345300712 1105 26-27 -0.892422117585113 1105 28-29 -0.8748245766822436 1105 30-31 -0.7888291673690659 1105 32-33 -0.7587099864552229 1105 34-35 -0.7804345310998215 1105 36-37 -0.7560693006290151 1105 38-39 -0.8224271091198219 1105 40-41 -0.8125745189490416 1105 42-43 -0.843224068757003 1105 44-45 -0.9067207753272157 1105 46-47 -0.9083676328131745 1105 48-49 -1.0054388155937062 1105 50-51 -0.9562970605153467 1105 52-53 -0.9732198723463412 1105 54-55 -0.9076997038258838 1105 56-57 -0.9385017259684929 1105 58-59 -1.046297406063566 1105 60-61 -0.9994678868877855 1105 62-63 -1.008470945325179 1105 64-65 -0.9528719211941805 1105 66-67 -1.0596745036168258 1105 68-69 -0.9343770078921878 1105 70-71 -0.9759399844214371 1105 72-73 -0.9463283166488026 1105 74-75 -1.0123550389174696 1105 76-77 -1.0113610271396034 1105 78-79 -0.9491667032566333 1105 80-81 -0.8251653781355728 1105 82-83 -0.8648782920252529 1105 84-85 -0.9011417205974332 1105 86-87 -0.8513448503093528 1105 88-89 -0.9061734597710611 1105 90-91 -0.872917749138356 1105 92-93 -1.0041470061451996 1105 94-95 -0.9262533768485604 1105 96-97 -1.0169239340877745 1105 98-99 -1.134681829445583 1105 100 -1.1420334927041793 1106 1 -0.4521329557308853 1106 2 -0.3967745277346175 1106 3 -0.46214403808588855 1106 4 -0.2756047106004189 1106 5 -0.3342220908319078 1106 6 -0.3878381803181554 1106 7 -0.44604433999317195 1106 8 -0.43511148127018373 1106 9 -0.5919339025712489 1106 10-11 -0.5931660910124847 1106 12-13 -0.6737779978884149 1106 14-15 -0.7667664413890307 1106 16-17 -0.7835681707690227 1106 18-19 -0.7843192313835274 1106 20-21 -0.8115795446981124 1106 22-23 -0.8222985756015007 1106 24-25 -0.8231392738643635 1106 26-27 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0.8248968334604712 2311 32-33 0.8946809239101228 2311 34-35 0.9847272671734331 2311 36-37 1.0150653866995825 2311 38-39 0.9956768046277844 2311 40-41 1.0152503635109653 2311 42-43 0.9801293224390264 2311 44-45 1.0149210462630407 2311 46-47 0.6667138751710837 2311 48-49 0.8677982123703103 2311 50-51 1.0234784324636266 2311 52-53 0.9270461318190684 2311 54-55 0.9804831477844687 2311 56-57 1.0942953353951665 2311 58-59 1.1154295152003257 2311 60-61 1.1673648485128325 2311 62-63 1.0939496972700056 2311 64-65 1.195270364450515 2311 66-67 1.2348595028257066 2311 68-69 1.2635788629338833 2311 70-71 1.1953697210191052 2311 72-73 1.2243781691645381 2311 74-75 1.216268546748239 2311 76-77 1.1444196314936868 2311 78-79 0.8460572665458912 2311 80-81 0.8586662509360323 2311 82-83 0.9509709028974029 2311 84-85 1.0267294692443265 2311 86-87 1.04903339414928 2311 88-89 1.0912770547307673 2311 90-91 1.1329647720911566 2311 92-93 1.1647628888396717 2311 94-95 1.112370074938891 2311 96-97 1.2208713143766339 2311 98-99 1.2904028592132768 2311 100 1.4310476651500572 2312 1 0.2641656684285678 2312 2 0.36128612212463196 2312 3 0.45224931878608743 2312 4 0.18194387054251848 2312 5 0.3797732427354319 2312 6 0.43110478321071355 2312 7 0.3754073197564338 2312 8 0.46011957597276876 2312 9 0.6327674388560709 2312 10-11 0.6607352738158241 2312 12-13 0.7010468525861384 2312 14-15 0.9085635263313705 2312 16-17 0.8790382314664598 2312 18-19 0.8587822532027829 2312 20-21 0.8837595562909257 2312 22-23 0.9694705254563658 2312 24-25 0.9517499843930821 2312 26-27 1.0224266519520455 2312 28-29 0.994375492282785 2312 30-31 1.0257336576638352 2312 32-33 0.8920104307128511 2312 34-35 1.0087764526370933 2312 36-37 1.0570367436634243 2312 38-39 0.8588767240692405 2312 40-41 1.0270489671629477 2312 42-43 1.1750360983072667 2312 44-45 0.8587470398183541 2312 46-47 0.9248697335670713 2312 48-49 1.125264691067052 2312 50-51 0.9448918544113596 2312 52-53 1.0891978727786125 2312 54-55 1.0157838325320512 2312 56-57 1.037052182870994 2312 58-59 1.1359234509325162 2312 60-61 1.1399808080545455 2312 62-63 1.1669487350430074 2312 64-65 1.1754914752632644 2312 66-67 1.1609836972403187 2312 68-69 1.265154923621317 2312 70-71 1.2064563885808681 2312 72-73 1.127489362326017 2312 74-75 1.1846087051800396 2312 76-77 1.126696305321115 2312 78-79 1.0447638320668347 2312 80-81 0.9667689854508907 2312 82-83 0.9834310935526105 2312 84-85 1.0314966774433962 2312 86-87 1.1292316129104627 2312 88-89 1.0922596898900991 2312 90-91 1.1840479935376322 2312 92-93 1.2453809070996051 2312 94-95 1.2494230645557884 2312 96-97 0.9488306770690791 2312 98-99 0.6979939574946954 2312 100 0.9015757711292913 2313 1 0.11240056683225674 2313 2 0.2899955042756588 2313 3 0.3442336037520377 2313 4 0.23449599207211236 2313 5 0.36272855138421534 2313 6 0.28748150290413577 2313 7 0.29409519712402954 2313 8 0.4126413702436764 2313 9 0.650903029272655 2313 10-11 0.42743106384091334 2313 12-13 0.501149717133579 2313 14-15 0.5076068667410638 2313 16-17 0.7636903226688005 2313 18-19 0.7801583387808009 2313 20-21 0.6993729113128424 2313 22-23 0.7160759126803171 2313 24-25 0.5984926989584451 2313 26-27 0.8668953735202365 2313 28-29 0.7267559339579535 2313 30-31 0.915760621353833 2313 32-33 1.0070455276222248 2313 34-35 1.0220744041686203 2313 36-37 1.0569479123656862 2313 38-39 1.0391729386759536 2313 40-41 1.074901879958297 2313 42-43 1.1127894549833997 2313 44-45 0.9544962058187423 2313 46-47 0.990744040864918 2313 48-49 1.0557262443740996 2313 50-51 0.9901603609317178 2313 52-53 1.0672435842285282 2313 54-55 1.0065033053891597 2313 56-57 0.7386869323954031 2313 58-59 0.760841815466172 2313 60-61 0.6383947199952118 2313 62-63 0.9937979534268422 2313 64-65 1.0805435278672704 2313 66-67 1.1218195992005562 2313 68-69 1.1929298126511227 2313 70-71 1.0992247986054053 2313 72-73 1.127950171236705 2313 74-75 1.1680777300208263 2313 76-77 1.1923317992418738 2313 78-79 1.0250943164758652 2313 80-81 0.9155292898262886 2313 82-83 0.9724953046303284 2313 84-85 0.9102736893813095 2313 86-87 0.9698945116449984 2313 88-89 1.0101754076811673 2313 90-91 0.9287112506852111 2313 92-93 1.1152745962727728 2313 94-95 1.158321137396353 2313 96-97 1.2387808852243545 2313 98-99 1.215845629629456 2313 100 1.3244226736133697 2314 1 -0.40671889583448717 2314 2 0.004009248987969727 2314 3 0.25690350934851836 2314 4 0.2064596165934276 2314 5 0.28755604413979086 2314 6 0.35316005722430077 2314 7 0.42797793736595935 2314 8 0.4452985254593713 2314 9 0.4498533259250834 2314 10-11 0.5513264748791542 2314 12-13 0.3854305457459972 2314 14-15 0.70342518772906 2314 16-17 0.7651531959074376 2314 18-19 0.8059874258083184 2314 20-21 0.8242944613168461 2314 22-23 0.8701870224035346 2314 24-25 0.7869343723173188 2314 26-27 0.7290532584622866 2314 28-29 0.7508719151994256 2314 30-31 0.7926472410488046 2314 32-33 0.8502197073000346 2314 34-35 0.9133550663915244 2314 36-37 0.8447848386216137 2314 38-39 0.5874769962558943 2314 40-41 0.7039903252366742 2314 42-43 0.8467551211889486 2314 44-45 0.8923529728519597 2314 46-47 0.8138081367740071 2314 48-49 0.8780839464194301 2314 50-51 0.8442257596543925 2314 52-53 0.9008733881592761 2314 54-55 0.8251123469966046 2314 56-57 0.8435384543904192 2314 58-59 0.9484371211630318 2314 60-61 1.0432191990819177 2314 62-63 0.8566337504792756 2314 64-65 0.8546943655254395 2314 66-67 0.5919759552774977 2314 68-69 0.6096851527558798 2314 70-71 0.8016164067972511 2314 72-73 0.9370809976170733 2314 74-75 0.9873711084716987 2314 76-77 0.8524365577027488 2314 78-79 0.761784802089231 2314 80-81 0.7970427872206329 2314 82-83 0.8650696572119898 2314 84-85 0.8361333347370952 2314 86-87 0.908977875639426 2314 88-89 0.87966491968659 2314 90-91 1.0370791202526988 2314 92-93 0.8392376119465403 2314 94-95 0.8414247061484552 2314 96-97 0.8543081104654284 2314 98-99 1.0266622975267836 2314 100 1.0636183360383953 2315 1 0.40261309088614894 2315 2 0.41955090111522964 2315 3 0.451559380387728 2315 4 0.3117097843002128 2315 5 0.3893534869805393 2315 6 0.47353642700191756 2315 7 0.411342043629773 2315 8 0.29941212973911746 2315 9 0.5250216266870424 2315 10-11 0.5679830853364152 2315 12-13 0.4179773616102338 2315 14-15 0.6587654068025728 2315 16-17 0.8190131585171443 2315 18-19 0.8096739639992876 2315 20-21 0.5379166945163618 2315 22-23 0.9123546711388499 2315 24-25 0.9642259381378224 2315 26-27 0.8660017086904048 2315 28-29 0.8470668356308906 2315 30-31 0.9247554884214253 2315 32-33 0.8771459543268136 2315 34-35 0.9357909344267838 2315 36-37 0.9497962811107286 2315 38-39 0.9726973227939837 2315 40-41 0.9700878734636049 2315 42-43 1.0704225675604775 2315 44-45 1.0344250901803917 2315 46-47 1.0044153944338206 2315 48-49 0.9564278813836964 2315 50-51 1.0445121937607595 2315 52-53 1.1011807972355427 2315 54-55 0.9462142875196236 2315 56-57 0.945151393937671 2315 58-59 0.9213797353808033 2315 60-61 1.0441311376773683 2315 62-63 1.1029142597377017 2315 64-65 1.189678657798659 2315 66-67 1.190684551017469 2315 68-69 1.1516121807271205 2315 70-71 1.0537387834798224 2315 72-73 1.1366055408627034 2315 74-75 0.51638110332118 2315 76-77 0.571078667798151 2315 78-79 0.8451427362060357 2315 80-81 0.8959330403520056 2315 82-83 0.851599687522139 2315 84-85 0.9215488767301103 2315 86-87 0.9565943060910591 2315 88-89 0.9609485475578765 2315 90-91 0.9725823067333437 2315 92-93 1.0550374509232263 2315 94-95 1.1583694603193244 2315 96-97 1.229330190740317 2315 98-99 1.2651331555926077 2315 100 1.1944634832226662 2316 1 0.281508172335279 2316 2 0.09867359302365486 2316 3 0.23797381888707747 2316 4 0.004799455272873843 2316 5 0.2605470151790641 2316 6 0.3265704520282853 2316 7 0.4212927017632566 2316 8 0.4383122005355915 2316 9 0.640738651476596 2316 10-11 0.6312457182768085 2316 12-13 0.3751670090670203 2316 14-15 0.5028771578146447 2316 16-17 0.7827820152847167 2316 18-19 0.7443215700872017 2316 20-21 0.737677616438269 2316 22-23 0.7758476103621277 2316 24-25 0.4638979289869454 2316 26-27 0.7423575005844327 2316 28-29 0.9147118305026041 2316 30-31 0.5208840472714655 2316 32-33 0.6672635108986711 2316 34-35 0.7490072406756596 2316 36-37 0.8327916895010432 2316 38-39 0.8112753463571423 2316 40-41 0.8165178357594058 2316 42-43 0.7396942534912299 2316 44-45 0.652176352344263 2316 46-47 0.829449382548141 2316 48-49 0.8981842394836335 2316 50-51 0.9208884739140615 2316 52-53 0.9470367357409017 2316 54-55 0.8515336969932505 2316 56-57 0.9742426069214289 2316 58-59 0.8169665788492608 2316 60-61 0.902960312460884 2316 62-63 0.8744721699684419 2316 64-65 0.9467277698980539 2316 66-67 0.9660159714220313 2316 68-69 0.9523993612149937 2316 70-71 0.9053292053430297 2316 72-73 0.916352813622396 2316 74-75 0.8838437879384387 2316 76-77 0.7979496937912778 2316 78-79 0.5727790443046601 2316 80-81 0.5471021983052893 2316 82-83 0.5226743299003971 2316 84-85 0.6571121192999065 2316 86-87 0.7520624711102961 2316 88-89 0.6891541928613236 2316 90-91 0.5005843551445324 2316 92-93 0.3507072739154573 2316 94-95 0.4039972388321189 2316 96-97 0.6199005938323054 2316 98-99 0.8285286926359205 2316 100 0.4219546419581519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 8.0 12 20.0 13 155.0 14 575.0 15 1746.0 16 4115.0 17 8192.0 18 13522.0 19 20391.0 20 30180.0 21 42998.0 22 53565.0 23 58147.0 24 61223.0 25 67783.0 26 81165.0 27 100123.0 28 125155.0 29 155285.0 30 191716.0 31 240123.0 32 305638.0 33 402409.0 34 572081.0 35 805755.0 36 1260133.0 37 1860802.0 38 1481976.0 39 211247.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.194011570605774 20.21734290661026 13.758226715428476 23.830418807355485 2 15.602954006770764 19.627907159226723 40.63609426103486 24.133044572967655 3 19.220366629541736 24.910179244269788 29.114393831278218 26.755060294910255 4 12.809014808623576 15.997397498925334 37.78710143070105 33.40648626175004 5 13.578063628307344 37.76297030839195 34.29169112645478 14.36727493684593 6 32.07193163912213 36.67130360494365 17.047687636613126 14.209077119321098 7 28.499836933466405 31.62378711602736 21.557211832009592 18.31916411849665 8 26.662186663371035 36.03347325067764 18.958248429056315 18.346091656895013 9 26.366178144337788 15.72890505388983 20.45597842493914 37.44893837683325 10-11 26.220847291766198 26.039880898120515 26.75423225038147 20.985039559731813 12-13 26.021485996768735 23.250970039206166 27.686615497270957 23.040928466754146 14-15 23.250170529143336 24.76814503954983 25.161758241138475 26.819926190168363 16-17 22.567978006975657 27.662425834998984 26.188814736233596 23.580781421791766 18-19 22.212503696554446 27.26550358228616 28.2641985698319 22.2577941513275 20-21 23.288156280296963 26.452162254861843 28.026888607100638 22.232792857740556 22-23 23.423569721553207 26.137521143310572 27.821782918706205 22.617126216430012 24-25 22.977936037829398 26.170018474298555 28.27528298731418 22.57676250055786 26-27 22.90357618261715 26.412184530292315 27.658087805722538 23.026151481368004 28-29 22.409496198734907 26.73556858117817 28.078541659923356 22.776393560163566 30-31 23.17602831123799 26.40968742913623 27.965476444523514 22.448807815102263 32-33 23.216462730481243 26.554781960813745 27.499539546403547 22.729215762301468 34-35 23.217096628116636 26.567818916137202 27.88172485757971 22.33335959816645 36-37 23.03978014508923 26.31297707395906 27.655416084469692 22.991826696482022 38-39 23.084440919169626 26.415151143909576 27.977685059452835 22.522722877467963 40-41 22.902031357827052 25.85818877311593 28.35120462233049 22.888575246726532 42-43 22.882576689192586 26.009914624813362 27.897734420076713 23.209774265917336 44-45 22.885036122115345 26.517694425940853 27.80135304275214 22.795916409191666 46-47 22.903005848529062 26.411945476677467 27.339376369862823 23.345672304930645 48-49 23.34925111628319 26.391671869908826 27.40101808827825 22.858058925529726 50-51 23.120518954307514 26.332519263798627 27.39105489751908 23.155906884374783 52-53 22.845903754827287 26.677061506473958 27.164730396819696 23.312304341879056 54-55 22.70952008805474 26.582944035174812 27.23955341635459 23.46798246041586 56-57 23.321420120374537 26.351228082651133 26.927580334493594 23.399771462480736 58-59 22.670388465285317 26.661864214023446 27.410409224142317 23.257338096548917 60-61 22.88499815501062 26.966804965770987 27.02047794144919 23.127718937769203 62-63 23.055272060467907 26.598951119943187 26.782040448605027 23.56373637098388 64-65 22.556771373179725 26.59139586729939 27.02194837196587 23.829884387555023 66-67 22.778547351456453 26.480049871564038 27.00832507446476 23.733077702514745 68-69 22.51580279524784 26.450160867953333 27.209361182598435 23.824675154200392 70-71 22.4370565758792 26.152769311938123 27.30482407906828 24.105350033114398 72-73 22.71635813981843 26.353038352742985 27.23832480083861 23.692278706599975 74-75 22.839525970737164 26.536232802144532 27.009318897681496 23.614922329436812 76-77 22.632847664250587 26.41587868084428 26.79776052589622 24.153513129008918 78-79 22.54212692633401 26.410159757243353 26.886309475489757 24.161403840932884 80-81 22.262322807174996 26.805577675858867 27.086672068720603 23.84542744824554 82-83 22.80025788898864 26.16509838165645 27.683492473832565 23.35115125552235 84-85 22.885516267137255 26.42333847600769 26.933633495088166 23.75751176176689 86-87 22.095107883926868 26.827923649204415 27.232440071470766 23.84452839539795 88-89 22.498492739014047 26.082706606404038 27.225261964045107 24.193538690536805 90-91 22.45185533690301 26.096749994894065 27.234278461625365 24.21711620657756 92-93 22.560729630078903 26.195749989911764 27.16094552439985 24.082574855609483 94-95 21.922192093522668 26.083108083376743 27.57388706432013 24.420812758780457 96-97 21.993819725187066 26.025852543444365 27.402915025941805 24.577412705426763 98-99 21.60810869641196 26.346763824191804 27.253004645926676 24.792122833469556 100 21.87576384512014 26.02085274948676 26.98031274095807 25.123070664435026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 652.0 1 471.5 2 440.0 3 617.0 4 530.0 5 470.0 6 741.0 7 1431.0 8 2312.5 9 2860.0 10 2773.0 11 2614.5 12 3021.5 13 3664.0 14 4271.0 15 4962.0 16 5807.0 17 6628.5 18 7430.5 19 8173.5 20 9067.0 21 10126.5 22 11464.0 23 13409.5 24 15741.0 25 18638.5 26 23073.5 27 28393.0 28 35198.0 29 46441.0 30 61638.0 31 77720.5 32 98293.5 33 120856.5 34 140642.5 35 161018.5 36 182580.5 37 211373.0 38 229088.5 39 230049.5 40 227738.5 41 225529.0 42 231344.5 43 245243.5 44 263552.0 45 294637.5 46 324907.5 47 346064.5 48 363980.0 49 366503.0 50 358833.0 51 363729.5 52 364130.5 53 347683.5 54 331599.0 55 300137.5 56 265959.0 57 228881.0 58 192349.5 59 174457.5 60 144269.0 61 107563.0 62 83419.5 63 66333.0 64 48500.5 65 30168.0 66 20226.0 67 14790.0 68 10888.0 69 7920.5 70 5942.5 71 4406.5 72 3173.5 73 2318.0 74 1720.0 75 1159.5 76 748.5 77 430.5 78 231.0 79 133.5 80 92.5 81 61.5 82 42.0 83 24.5 84 15.5 85 12.0 86 10.0 87 10.5 88 8.0 89 3.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04522915204596626 2 0.0 3 0.0 4 0.03125213026976633 5 0.0 6 0.0 7 7.84674906734031E-4 8 0.0 9 0.01105901196678275 10-11 4.107282714935944E-4 12-13 1.839081812657885E-5 14-15 1.6551736313920966E-4 16-17 2.636017264809635E-4 18-19 0.0 20-21 1.839081812657885E-5 22-23 0.0 24-25 5.885061800505232E-4 26-27 4.0459799878473474E-4 28-29 1.471265450126308E-4 30-31 0.002323373356657795 32-33 0.015227597408807289 34-35 0.026010747103691355 36-37 0.030688145180551244 38-39 0.022032200115641467 40-41 0.0012076637236453445 42-43 1.2260545417719235E-4 44-45 0.011298092602428275 46-47 0.017882005491743503 48-49 0.003328738080910772 50-51 0.011291962329719416 52-53 0.01122452932992196 54-55 0.010574720422782841 56-57 4.16858544202454E-4 58-59 0.008447515792808552 60-61 0.004542532077264976 62-63 0.001422223268455431 64-65 0.03534715243928455 66-67 0.06014410554662171 68-69 0.05931651873092565 70-71 0.051506551299838506 72-73 0.045180109864295374 74-75 0.03513259289447447 76-77 0.03119082754267773 78-79 0.015276639590478164 80-81 0.0010666674513415734 82-83 0.03829581361224603 84-85 0.05143298802733219 86-87 0.05712188110115391 88-89 0.0698605877901642 90-91 0.04860693230854791 92-93 0.039000794973764887 94-95 0.01280613968880774 96-97 0.0036291214436448933 98-99 0.02193411575229971 100 0.03181611535898141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8156244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.6128711061981 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71539811179319 47.40758462728182 2 14.564003884096884 18.237881959702534 3 4.64556022144194 8.72615590083676 4 1.9880375056244695 4.979069443758099 5 1.0032824327986205 3.140919682396644 6 0.5610402686008608 2.1077005213975477 7 0.3551486679610071 1.5565814439406338 8 0.23252304046812747 1.1647148129641718 9 0.1719122145085314 0.9687525595743379 >10 0.7155062566696555 7.495449963707294 >50 0.03247973076354204 1.381010230542739 >100 0.014119158480552393 1.6169368123930585 >500 6.083221542668626E-4 0.25939851715703 >1k 3.041477111893819E-4 0.2889860439696794 >5k 2.5345642363164944E-5 0.14800021026412463 >10k+ 5.069128472632989E-5 0.5208572701135358 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17533 0.21496414280887133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15288 0.18743921834609165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9326 0.11434184656564958 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.452109083543847E-5 0.0 5 0.0 0.0 0.0 2.452109083543847E-5 0.0 6 0.0 0.0 0.0 2.452109083543847E-5 1.2260545417719235E-5 7 0.0 0.0 0.0 2.452109083543847E-5 1.2260545417719235E-5 8 0.0 0.0 0.0 2.452109083543847E-5 1.2260545417719235E-5 9 0.0 0.0 0.0 3.67816362531577E-5 4.904218167087694E-5 10-11 0.0 0.0 0.0 4.291190896201732E-5 4.904218167087694E-5 12-13 0.0 0.0 0.0 4.904218167087694E-5 4.904218167087694E-5 14-15 0.0 0.0 0.0 4.904218167087694E-5 6.130272708859618E-5 16-17 0.0 0.0 0.0 6.130272708859618E-5 7.35632725063154E-5 18-19 0.0 0.0 0.0 9.195409063289425E-5 8.582381792403464E-5 20-21 0.0 0.0 0.0 1.2873572688605197E-4 9.808436334175386E-5 22-23 0.0 0.0 0.0 2.5747145377210393E-4 1.409962723037712E-4 24-25 0.0 0.0 0.0 4.6590072587333095E-4 1.777779085569289E-4 26-27 0.0 0.0 0.0 9.501922698732407E-4 1.9616872668350776E-4 28-29 0.0 0.0 0.0 0.0031386996269361237 1.9616872668350776E-4 30-31 0.0 0.0 0.0 0.012236024326883796 1.9616872668350776E-4 32-33 0.0 0.0 0.0 0.03554332116596806 2.3295036293666545E-4 34-35 0.0 0.0 0.0 0.0692230394284428 2.5747145377210393E-4 36-37 0.0 0.0 0.0 0.12856407925020388 2.5747145377210393E-4 38-39 0.0 0.0 0.0 0.21588368371520028 2.5747145377210393E-4 40-41 0.0 0.0 1.2260545417719235E-5 0.31093356206606865 2.5747145377210393E-4 42-43 0.0 0.0 1.2260545417719235E-5 0.4034025956065071 2.6973199918982316E-4 44-45 0.0 0.0 1.2260545417719235E-5 0.5106394561025884 2.88122817316402E-4 46-47 0.0 0.0 1.2260545417719235E-5 0.637946829447476 3.1264390815184044E-4 48-49 0.0 0.0 1.2260545417719235E-5 0.7761722184868427 3.4329527169613854E-4 50-51 0.0 0.0 1.2260545417719235E-5 0.9216435898680815 3.4329527169613854E-4 52-53 6.130272708859617E-6 0.0 1.2260545417719235E-5 1.0626276016264349 3.800769079492963E-4 54-55 1.2260545417719235E-5 0.0 2.452109083543847E-5 1.2155595148943559 4.16858544202454E-4 56-57 1.2260545417719235E-5 0.0 2.452109083543847E-5 1.3960224828977652 4.16858544202454E-4 58-59 1.2260545417719235E-5 0.0 2.452109083543847E-5 1.6022571173692204 4.16858544202454E-4 60-61 1.2260545417719235E-5 0.0 2.452109083543847E-5 1.808387537204625 4.16858544202454E-4 62-63 1.2260545417719235E-5 0.0 2.452109083543847E-5 2.015425237400941 4.291190896201732E-4 64-65 1.2260545417719235E-5 0.0 2.452109083543847E-5 2.227262941128294 4.291190896201732E-4 66-67 1.2260545417719235E-5 0.0 2.452109083543847E-5 2.4662454924104775 4.291190896201732E-4 68-69 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 2.73119587888739 4.291190896201732E-4 70-71 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 3.018080381116602 4.291190896201732E-4 72-73 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 3.304646109164954 5.394639983796463E-4 74-75 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 3.5786876900691054 5.394639983796463E-4 76-77 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 3.8889714432280345 5.45594271088506E-4 78-79 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 4.227478971938554 5.517245437973655E-4 80-81 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 4.588662379399144 5.701153619239443E-4 82-83 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 4.95530785984333 6.007667254682425E-4 84-85 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 5.311059845683872 6.007667254682425E-4 86-87 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 5.68429536929008 6.007667254682425E-4 88 1.2260545417719235E-5 1.2260545417719235E-5 2.452109083543847E-5 5.987314749289011 6.130272708859617E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12780 0.0 45.949577 1 GTATCAA 21640 0.0 38.15199 1 CAACGCA 24955 0.0 31.387663 5 GTACTAG 5500 0.0 31.372803 1 ATCAACG 25635 0.0 30.583145 3 TATCAAC 27850 0.0 29.345613 2 CTGTGCG 8200 0.0 28.70777 9 AACGCAG 28205 0.0 28.45396 6 TAGTACT 8560 0.0 28.113546 4 TCAACGC 28585 0.0 27.607807 4 GTACATG 31955 0.0 26.77827 1 GTGGTAT 4870 0.0 26.64588 1 GGGCACC 7765 0.0 26.517466 94 TACCTGG 5105 0.0 26.237226 2 CTAGTAC 9285 0.0 26.171465 3 TACATGG 32385 0.0 26.106949 2 GTACTGT 9890 0.0 25.94044 6 ACGCAGA 30715 0.0 25.547411 7 ACATGGG 31990 0.0 25.46266 3 CGCAGAG 31540 0.0 24.84906 8 >>END_MODULE