FastQCFastQC Report
Mon 6 Jun 2016
ERR1211217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8740188
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245880.28132117981901533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188890.21611663273146983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121590.13911600070845156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144500.045.662941
GTATCAA253050.039.05681
GTACTAG68800.033.8610531
CAACGCA283350.033.313425
ATCAACG291050.032.383813
GCATCGT66350.031.809318
TATCAAC314900.031.0117762
AACGCAG321650.030.0634886
TACCTGG55150.028.929132
TCAACGC336450.028.4185334
GTACATG361250.028.4009131
TACATGG362450.027.8782252
CTGTGCG91500.027.4321029
TAGGCAT89500.027.0497265
GGGCACC80000.027.02291794
ACATGGG363200.026.8049773
ACGCAGA349900.026.8034117
CGCAGAG355900.026.348988
TAGTACT98550.026.0439744
GTGGTAT52800.025.6709691
Produced by FastQC (version 0.11.5)