FastQCFastQC Report
Mon 6 Jun 2016
ERR1211217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8740188
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229290.2623398947482594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190670.218153202196566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121400.1388986140801548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156300.044.878471
GTATCAA264500.038.002331
GTACTAG70050.036.242141
CAACGCA307800.031.2799075
TACCTGG55300.031.188372
ATCAACG311050.030.9768713
TATCAAC340850.029.2848472
AACGCAG346700.028.4208266
GTGGTAT55050.027.5850281
TCAACGC360700.026.9996094
GGGCACC82400.026.75633694
GTACATG363650.026.7359241
GCATCGT86750.026.4867698
TACATGG364850.026.0575312
CTGTGCG93950.026.0573679
ACGCAGA377850.025.5306367
ACATGGG360100.025.230313
CGCAGAG383500.025.11768
TAGTACT99600.024.9637724
ACCTGGG68500.024.7016433
Produced by FastQC (version 0.11.5)