##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211217_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8740188 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16783334637653 33.0 31.0 34.0 30.0 34.0 2 32.309519886757585 34.0 31.0 34.0 31.0 34.0 3 32.38869438506357 34.0 31.0 34.0 31.0 34.0 4 36.00009816722478 37.0 35.0 37.0 35.0 37.0 5 35.88788547797828 37.0 35.0 37.0 35.0 37.0 6 35.91173153254827 37.0 35.0 37.0 35.0 37.0 7 35.846099992357146 37.0 35.0 37.0 35.0 37.0 8 35.83340392678052 37.0 35.0 37.0 35.0 37.0 9 37.43743738693035 39.0 37.0 39.0 34.0 39.0 10-11 37.41633183405208 39.0 37.0 39.0 34.0 39.0 12-13 37.31535574520822 39.0 37.0 39.0 34.0 39.0 14-15 38.548629045507944 40.0 38.0 41.0 34.0 41.0 16-17 38.50347389552719 40.0 38.0 41.0 34.0 41.0 18-19 38.5177378335569 40.0 38.0 41.0 34.0 41.0 20-21 38.476412120654615 40.0 38.0 41.0 34.0 41.0 22-23 38.34535241118384 40.0 38.0 41.0 34.0 41.0 24-25 38.28410527325042 40.0 38.0 41.0 34.0 41.0 26-27 38.14824641071794 40.0 38.0 41.0 33.0 41.0 28-29 38.12860644416344 40.0 37.5 41.0 33.5 41.0 30-31 38.14523983923458 40.0 37.5 41.0 33.5 41.0 32-33 38.023643827798665 40.0 37.0 41.0 33.0 41.0 34-35 37.89036294184976 40.0 37.0 41.0 33.0 41.0 36-37 37.752250066016884 40.0 37.0 41.0 33.0 41.0 38-39 37.66039597775242 40.0 37.0 41.0 33.0 41.0 40-41 37.561448907048685 40.0 36.5 41.0 32.5 41.0 42-43 37.414969391962735 40.0 36.0 41.0 32.0 41.0 44-45 37.26638551710787 40.0 36.0 41.0 31.5 41.0 46-47 37.02557044539545 39.0 36.0 41.0 31.0 41.0 48-49 36.78523568371756 39.0 35.0 41.0 31.0 41.0 50-51 36.57700114688609 39.0 35.0 41.0 30.0 41.0 52-53 36.3646303717952 39.0 35.0 40.0 30.0 41.0 54-55 36.11961985257068 38.0 35.0 40.0 29.5 41.0 56-57 35.84961656431189 38.0 35.0 40.0 29.0 41.0 58-59 35.56335641750498 37.5 34.0 40.0 28.0 41.0 60-61 35.2947029857939 37.0 34.0 40.0 28.0 41.0 62-63 34.98163649340266 36.5 34.0 40.0 28.0 41.0 64-65 34.60613484515436 36.0 34.0 39.0 27.0 41.0 66-67 34.302941939006345 35.5 33.0 39.0 26.5 40.0 68-69 33.97981971326017 35.0 33.0 38.5 26.0 40.0 70-71 33.67695752082221 35.0 33.0 38.0 26.0 40.0 72-73 33.35200501407979 35.0 32.5 37.0 26.0 39.5 74-75 33.02715999930436 35.0 32.0 37.0 25.5 39.0 76-77 32.05120805181765 34.0 30.5 35.5 24.5 38.0 78-79 32.55625645581079 35.0 32.0 36.0 25.5 38.0 80-81 32.498573600476334 35.0 32.0 35.5 25.0 37.0 82-83 32.260647082190914 35.0 32.0 35.0 25.0 37.0 84-85 32.02554533151918 34.0 32.0 35.0 25.0 36.5 86-87 31.800552859961364 34.0 31.0 35.0 25.0 36.0 88-89 31.59860903449674 34.0 31.0 35.0 24.0 36.0 90-91 31.39192755350343 34.0 31.0 35.0 24.0 35.0 92-93 31.218583112857527 34.0 31.0 35.0 23.5 35.0 94-95 31.036104086090596 34.0 31.0 35.0 23.0 35.0 96-97 30.87310507508534 34.0 30.0 35.0 22.0 35.0 98-99 30.706507342862647 34.0 30.0 35.0 20.0 35.0 100 28.89581219534408 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3506881764912819 1101 2 -0.2977044861431217 1101 3 -0.3769166152290282 1101 4 -0.19824049691618484 1101 5 -0.24573210218734687 1101 6 -0.3265378390923175 1101 7 -0.3171677867075289 1101 8 -0.34488443175732897 1101 9 -0.4848199330570324 1101 10-11 -0.47147142252152463 1101 12-13 -0.44469837808500046 1101 14-15 -0.6347538069604113 1101 16-17 -0.6448100115822442 1101 18-19 -0.6997082961062446 1101 20-21 -0.7328159894646475 1101 22-23 -0.6936136657799068 1101 24-25 -0.7175983575741753 1101 26-27 -0.8440179870228306 1101 28-29 -0.9536537517082166 1101 30-31 -0.765007911727345 1101 32-33 -0.7356153842702398 1101 34-35 -0.7279895885148377 1101 36-37 -0.6502960653812835 1101 38-39 -0.7188317274342921 1101 40-41 -0.8081015249984489 1101 42-43 -0.7591428476640658 1101 44-45 -0.8890093782597077 1101 46-47 -0.7456318816660144 1101 48-49 -0.8239499618968935 1101 50-51 -0.8641221156084882 1101 52-53 -0.8388212143487266 1101 54-55 -0.8535218006830476 1101 56-57 -0.809874044324026 1101 58-59 -0.7732632913400082 1101 60-61 -0.8499847950483073 1101 62-63 -0.9383592149252067 1101 64-65 -0.8764865531731587 1101 66-67 -0.9819189790342975 1101 68-69 -0.9727020540998765 1101 70-71 -0.962480343198969 1101 72-73 -1.039578153618109 1101 74-75 -1.0989196611005951 1101 76-77 -1.0200652740815457 1101 78-79 -0.9491886653644954 1101 80-81 -0.8849118391445359 1101 82-83 -0.8443736429799102 1101 84-85 -0.8918761035494818 1101 86-87 -0.9359347416792048 1101 88-89 -0.9691479101907277 1101 90-91 -1.143849290000599 1101 92-93 -1.0827451483231485 1101 94-95 -1.0251927175674105 1101 96-97 -1.1149811608773312 1101 98-99 -1.1729455230983277 1101 100 -1.136975261792184 1102 1 -0.4267136913101055 1102 2 -0.355032998534778 1102 3 -0.3642633318536781 1102 4 -0.2518979567774764 1102 5 -0.38298363260556556 1102 6 -0.3605806741047104 1102 7 -0.37511842871489876 1102 8 -0.3830397761715503 1102 9 -0.5207773738379231 1102 10-11 -0.5433906661199828 1102 12-13 -0.5822439942006383 1102 14-15 -0.681970227204836 1102 16-17 -0.7335140908275974 1102 18-19 -0.6888022194796122 1102 20-21 -0.7021244650050633 1102 22-23 -0.7174452074887583 1102 24-25 -0.7759362358682935 1102 26-27 -0.8107519228613072 1102 28-29 -0.8830714957979353 1102 30-31 -0.7651026570397548 1102 32-33 -0.733502516899982 1102 34-35 -0.7203359965136755 1102 36-37 -0.8311816582589913 1102 38-39 -0.8808258554763029 1102 40-41 -0.7506339902771089 1102 42-43 -0.7454884065197618 1102 44-45 -0.7270097155905404 1102 46-47 -0.831912263251354 1102 48-49 -0.8915233036803798 1102 50-51 -0.8707565857542434 1102 52-53 -0.8577482321283867 1102 54-55 -0.8454735147195578 1102 56-57 -0.8494706900773039 1102 58-59 -0.9560869886998447 1102 60-61 -0.9295772363610979 1102 62-63 -0.9059145269571687 1102 64-65 -0.8915139101785599 1102 66-67 -1.0298616549308477 1102 68-69 -1.0143355364553983 1102 70-71 -1.0410900598654536 1102 72-73 -1.0569838524829862 1102 74-75 -1.170743596543204 1102 76-77 -0.9124038304143873 1102 78-79 -0.806262594287503 1102 80-81 -0.8248457500678086 1102 82-83 -0.7178654336269155 1102 84-85 -0.835691335931589 1102 86-87 -0.8400987324943543 1102 88-89 -0.8757500449808475 1102 90-91 -0.9042009189351781 1102 92-93 -1.0089001358946277 1102 94-95 -1.0861530241594899 1102 96-97 -1.1711829076056617 1102 98-99 -1.219710918132808 1102 100 -1.2351102050078495 1103 1 -0.34252491756998893 1103 2 -0.35848677261506623 1103 3 -0.3897436470767346 1103 4 -0.27969031034931646 1103 5 -0.2757809434091456 1103 6 -0.30116408884740054 1103 7 -0.38696638798052874 1103 8 -0.33529884048826375 1103 9 -0.5619618020284776 1103 10-11 -0.5060490635502646 1103 12-13 -0.5749716387644312 1103 14-15 -0.7432913498233091 1103 16-17 -0.723139048963958 1103 18-19 -0.7175482121281505 1103 20-21 -0.7414985137935517 1103 22-23 -0.7924465324660304 1103 24-25 -0.8804710849004422 1103 26-27 -0.765593267639936 1103 28-29 -0.8282345237575228 1103 30-31 -0.8328350251631633 1103 32-33 -0.7618277744498769 1103 34-35 -0.8874769611898401 1103 36-37 -0.8639438981985279 1103 38-39 -0.8384299501384405 1103 40-41 -0.8211799617260098 1103 42-43 -0.7721927438876648 1103 44-45 -0.8256514071561298 1103 46-47 -0.8745705172207394 1103 48-49 -0.9289044676281222 1103 50-51 -0.967586472705797 1103 52-53 -1.0246664095510525 1103 54-55 -1.024447121548242 1103 56-57 -0.9744322563395116 1103 58-59 -0.9963913563386271 1103 60-61 -1.0163818221982623 1103 62-63 -1.0056800373039536 1103 64-65 -1.029384075311448 1103 66-67 -1.0703332735418698 1103 68-69 -1.1109951810557632 1103 70-71 -1.1531525182289073 1103 72-73 -1.0418634029654967 1103 74-75 -0.9919426596245273 1103 76-77 -0.9571414529653879 1103 78-79 -0.8869301861203276 1103 80-81 -0.8557910013805987 1103 82-83 -0.9055134755611007 1103 84-85 -0.9099397887778728 1103 86-87 -0.9666791829214709 1103 88-89 -0.9920041456758639 1103 90-91 -1.0418818754347008 1103 92-93 -1.1606257731335177 1103 94-95 -1.1767889399518232 1103 96-97 -1.1725778455535014 1103 98-99 -1.2001992966099628 1103 100 -1.3226330037006377 1104 1 -0.3441435889333313 1104 2 -0.3289319199318044 1104 3 -0.41245383228072185 1104 4 -0.2577537070418643 1104 5 -0.30588730209755965 1104 6 -0.33081087983529045 1104 7 -0.3627115154361107 1104 8 -0.35131635049437193 1104 9 -0.5545373969838465 1104 10-11 -0.4765641706543633 1104 12-13 -0.5329164579511954 1104 14-15 -0.7007537560338548 1104 16-17 -0.6077394200851529 1104 18-19 -0.6720663018718227 1104 20-21 -0.7530524036320188 1104 22-23 -0.6981215156385332 1104 24-25 -0.7494168082400705 1104 26-27 -0.7755144632019366 1104 28-29 -0.8506559236268671 1104 30-31 -0.8235650437811373 1104 32-33 -0.8023668090394764 1104 34-35 -0.8405472548030204 1104 36-37 -0.8113813454810455 1104 38-39 -0.7600580555481571 1104 40-41 -0.8138301288618024 1104 42-43 -0.6882245306433035 1104 44-45 -0.9216576567320445 1104 46-47 -0.9250907394377919 1104 48-49 -0.9355722544768739 1104 50-51 -0.878389384469294 1104 52-53 -0.8791249158439598 1104 54-55 -0.9581572203240327 1104 56-57 -0.8927243942957546 1104 58-59 -0.8877969264749836 1104 60-61 -0.8335043996703675 1104 62-63 -0.9204279071832175 1104 64-65 -0.9407144095780708 1104 66-67 -1.0579961436415246 1104 68-69 -1.0039959581666054 1104 70-71 -1.0855750164050022 1104 72-73 -1.0396795431961081 1104 74-75 -1.0710395666837549 1104 76-77 -0.9646694665977655 1104 78-79 -0.7777234842946399 1104 80-81 -0.8384130521112247 1104 82-83 -0.8893950191538451 1104 84-85 -0.8702066847475471 1104 86-87 -0.8905552572685416 1104 88-89 -0.9207401035702247 1104 90-91 -0.9090857548473075 1104 92-93 -1.0379794246877125 1104 94-95 -1.0569983747906626 1104 96-97 -1.1180144119618056 1104 98-99 -1.115711461688317 1104 100 -1.221869055204909 1105 1 -0.44518664310138334 1105 2 -0.40937404724893156 1105 3 -0.4453228729622616 1105 4 -0.32312063178379447 1105 5 -0.3398524688752289 1105 6 -0.38817972626664243 1105 7 -0.43598893246353043 1105 8 -0.5204614415144633 1105 9 -0.6410420381759891 1105 10-11 -0.6000543957070903 1105 12-13 -0.5356298903521193 1105 14-15 -0.7834150183826125 1105 16-17 -0.7026354095123963 1105 18-19 -0.6864286756091929 1105 20-21 -0.8160068103364253 1105 22-23 -0.8463994529789716 1105 24-25 -0.8165658050765643 1105 26-27 -0.8829374221873962 1105 28-29 -0.8553430767515167 1105 30-31 -0.774495623617959 1105 32-33 -0.7836832356684056 1105 34-35 -0.8482630633000738 1105 36-37 -0.8523828786700776 1105 38-39 -0.8556703425666896 1105 40-41 -0.9141044205423441 1105 42-43 -0.8677663429976121 1105 44-45 -0.8735252335996151 1105 46-47 -0.8500464261122289 1105 48-49 -0.8934910717706899 1105 50-51 -0.9359537818774442 1105 52-53 -0.9557180275933277 1105 54-55 -0.8848112954156093 1105 56-57 -0.8857474302875445 1105 58-59 -1.00179707563138 1105 60-61 -1.002211994159211 1105 62-63 -1.0000318969434474 1105 64-65 -0.8857285967797637 1105 66-67 -0.9037840363044225 1105 68-69 -0.8916139825033795 1105 70-71 -0.9162092259454511 1105 72-73 -0.9527803312968999 1105 74-75 -0.9592368728498073 1105 76-77 -1.0288162573070565 1105 78-79 -0.9187586643923176 1105 80-81 -0.9518668725962982 1105 82-83 -0.8814429018267305 1105 84-85 -0.87544753764465 1105 86-87 -0.8716262695895551 1105 88-89 -0.8291719495020686 1105 90-91 -0.866469083324386 1105 92-93 -0.9349466068111454 1105 94-95 -0.9342188106048468 1105 96-97 -1.0327676274962734 1105 98-99 -1.1228631641525233 1105 100 -1.1037762105495652 1106 1 -0.4849539393296922 1106 2 -0.43363976512334546 1106 3 -0.4761011196520961 1106 4 -0.32014362952089215 1106 5 -0.34164427757450255 1106 6 -0.39697472871522876 1106 7 -0.4490490171876047 1106 8 -0.43151141606644927 1106 9 -0.580047406431305 1106 10-11 -0.5749913980517718 1106 12-13 -0.7031110045853026 1106 14-15 -0.8285936697872103 1106 16-17 -0.8035210372435984 1106 18-19 -0.8581585161189693 1106 20-21 -0.7845456255271088 1106 22-23 -0.7294140578036021 1106 24-25 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1.2553239313524074 2310 72-73 1.2816800398845558 2310 74-75 1.231960862113013 2310 76-77 1.2216252385974933 2310 78-79 1.1067257689207892 2310 80-81 1.0150785687504964 2310 82-83 1.0389444966956205 2310 84-85 1.1045977140191248 2310 86-87 1.0101962160242408 2310 88-89 1.0515473765188688 2310 90-91 1.1377199926602124 2310 92-93 1.2156358009188466 2310 94-95 1.2120433166455165 2310 96-97 1.2272393537827035 2310 98-99 1.2641871336442811 2310 100 1.4389284640188507 2311 1 0.4223910946397851 2311 2 0.36816456628616123 2311 3 0.43864050920483777 2311 4 0.3106695478089776 2311 5 0.430187707313074 2311 6 0.4603352595435197 2311 7 0.5069258821697105 2311 8 0.49998497343907644 2311 9 0.6579162440902451 2311 10-11 0.6834603449809933 2311 12-13 0.7296118510767755 2311 14-15 0.8532138960806748 2311 16-17 0.7007526626433744 2311 18-19 0.8395636261959893 2311 20-21 0.758027885330911 2311 22-23 0.9675471927978521 2311 24-25 0.9473829174289108 2311 26-27 0.2775607645901701 2311 28-29 0.6191447372770895 2311 30-31 0.8790931422332662 2311 32-33 0.9629893173324362 2311 34-35 1.0001791944512277 2311 36-37 1.0260208285288925 2311 38-39 1.0374285604439564 2311 40-41 1.0063909592470708 2311 42-43 0.9993149374383776 2311 44-45 1.0074135294599458 2311 46-47 0.7191911540290761 2311 48-49 1.0148671482617786 2311 50-51 1.0981722745305902 2311 52-53 1.0102274099359505 2311 54-55 1.0238993727054506 2311 56-57 1.061156873715582 2311 58-59 1.15964002230033 2311 60-61 1.1821850412936143 2311 62-63 1.1779209814024654 2311 64-65 1.1832983741378698 2311 66-67 1.184335135237454 2311 68-69 1.2278920063054315 2311 70-71 1.2163562369675702 2311 72-73 1.278383399352073 2311 74-75 1.2814959383054187 2311 76-77 1.299387641359484 2311 78-79 0.9596673421061368 2311 80-81 0.9469265225214158 2311 82-83 1.0515170629301664 2311 84-85 1.1279048896766604 2311 86-87 1.150899427374199 2311 88-89 1.1985050558688215 2311 90-91 1.1963209352368018 2311 92-93 1.2284943640492827 2311 94-95 1.3206801837818567 2311 96-97 1.3083645453883257 2311 98-99 1.387412301987954 2311 100 1.5293580121683483 2312 1 0.25132010324433907 2312 2 0.3552218009393542 2312 3 0.4501822459126714 2312 4 0.19871872222010012 2312 5 0.36890040154485604 2312 6 0.40986960227778724 2312 7 0.2890118230392744 2312 8 0.41870500635074137 2312 9 0.5955962691719705 2312 10-11 0.6035353979601865 2312 12-13 0.6781178593288004 2312 14-15 0.8827592100822201 2312 16-17 0.8436971922096888 2312 18-19 0.8245681338141395 2312 20-21 0.87558207878795 2312 22-23 0.9094124608685945 2312 24-25 0.939288667127208 2312 26-27 0.9838218450847691 2312 28-29 0.97579543507279 2312 30-31 1.0176576984652428 2312 32-33 0.9378137897109937 2312 34-35 0.9905335549025622 2312 36-37 1.0629938528050005 2312 38-39 0.9038109171861635 2312 40-41 1.0020673365943082 2312 42-43 1.091775782563424 2312 44-45 0.8083837512714922 2312 46-47 0.8407934666044099 2312 48-49 1.076401282815631 2312 50-51 0.8880723026419304 2312 52-53 1.02645381706391 2312 54-55 0.9565239532645151 2312 56-57 1.0133405809327556 2312 58-59 1.061896269262128 2312 60-61 1.1081384323270314 2312 62-63 1.1364495319635424 2312 64-65 1.105397789715063 2312 66-67 1.1138750323588127 2312 68-69 1.2085370211673805 2312 70-71 1.11618339233096 2312 72-73 1.0754589900143188 2312 74-75 1.1562690385671957 2312 76-77 1.1323805077084153 2312 78-79 1.0366502230483619 2312 80-81 0.9373340965960608 2312 82-83 0.9754643512749581 2312 84-85 1.036374044136867 2312 86-87 1.1180138736302325 2312 88-89 1.1225480850979963 2312 90-91 1.1943522348160833 2312 92-93 1.1999642593110167 2312 94-95 1.2174914576496683 2312 96-97 0.9037333610840577 2312 98-99 0.7091666692797141 2312 100 0.9271348066240925 2313 1 0.03944792698312227 2313 2 0.21897849078258957 2313 3 0.28215778717707707 2313 4 0.18864656960306547 2313 5 0.3285653875118868 2313 6 0.28155618224609924 2313 7 0.24561347825365942 2313 8 0.4082866429571439 2313 9 0.6212168712110895 2313 10-11 0.354229913179573 2313 12-13 0.4323463044841489 2313 14-15 0.5106230593570231 2313 16-17 0.7410525792525533 2313 18-19 0.6929415050492551 2313 20-21 0.6105396518366817 2313 22-23 0.6392303416554626 2313 24-25 0.5383160236804301 2313 26-27 0.740878125799064 2313 28-29 0.5963044738719177 2313 30-31 0.8104373684955206 2313 32-33 0.8911494608026018 2313 34-35 0.9382869225441937 2313 36-37 0.9964398925152622 2313 38-39 0.9747985091685081 2313 40-41 0.9496789289814203 2313 42-43 1.0219435852529202 2313 44-45 0.8811534227051752 2313 46-47 0.9338241090207191 2313 48-49 1.0443634753856088 2313 50-51 0.9779954468825665 2313 52-53 0.9611607751229485 2313 54-55 0.9248499656241123 2313 56-57 0.6698131938607901 2313 58-59 0.7223586421482082 2313 60-61 0.634319272409094 2313 62-63 0.9719623839398679 2313 64-65 1.088866780547356 2313 66-67 1.005243697872558 2313 68-69 1.087892461549238 2313 70-71 1.0434011202623168 2313 72-73 1.0682096742374867 2313 74-75 1.1591370957006433 2313 76-77 1.1562188805761089 2313 78-79 0.9702918744554054 2313 80-81 0.9270954407632246 2313 82-83 0.9426360894484631 2313 84-85 0.9512793482642685 2313 86-87 1.0005654030068172 2313 88-89 1.0311636487473876 2313 90-91 0.8903031924686822 2313 92-93 1.1222991518485514 2313 94-95 1.2565866203977656 2313 96-97 1.3079705928761136 2313 98-99 1.259090182121529 2313 100 1.3477297828161383 2314 1 -0.3970721913801327 2314 2 0.026346099693107305 2314 3 0.27613808971591425 2314 4 0.2319891518123427 2314 5 0.3473005165078149 2314 6 0.40861751831901216 2314 7 0.45070793449297497 2314 8 0.4691001174060361 2314 9 0.48531461396024156 2314 10-11 0.6039166440341575 2314 12-13 0.4684729449371403 2314 14-15 0.7916104108485129 2314 16-17 0.8277171201626103 2314 18-19 0.8528926791899352 2314 20-21 0.8602339352967192 2314 22-23 0.9536706162124844 2314 24-25 0.8185560379715398 2314 26-27 0.7936279842863385 2314 28-29 0.7813634221237393 2314 30-31 0.8458843505076601 2314 32-33 0.894703786318054 2314 34-35 0.9095289537849638 2314 36-37 0.9162998330834355 2314 38-39 0.659976540476805 2314 40-41 0.6636432810259549 2314 42-43 0.8775624660858838 2314 44-45 0.9184917548841796 2314 46-47 0.8382343990211822 2314 48-49 0.84537003663673 2314 50-51 0.8099160199763631 2314 52-53 0.8299799514514774 2314 54-55 0.7185593981752376 2314 56-57 0.7404624135430922 2314 58-59 0.8546485295679034 2314 60-61 0.9216491726673652 2314 62-63 0.8511476799705306 2314 64-65 0.9084507453954345 2314 66-67 0.6431661843944667 2314 68-69 0.539080077147112 2314 70-71 0.7114127923601146 2314 72-73 0.8638171207395189 2314 74-75 0.9688988139933841 2314 76-77 0.815446696682212 2314 78-79 0.7513474180364383 2314 80-81 0.7920908123326811 2314 82-83 0.8118832261554516 2314 84-85 0.8381378347586441 2314 86-87 0.8907579156399237 2314 88-89 0.8711966802027504 2314 90-91 0.9744838340220703 2314 92-93 0.842191662255324 2314 94-95 0.8936153851111079 2314 96-97 0.925346670210839 2314 98-99 1.0710998533349887 2314 100 1.1073416163505172 2315 1 0.4177255881566637 2315 2 0.4290448561837721 2315 3 0.45904638149078636 2315 4 0.3245663125809912 2315 5 0.4105531974659655 2315 6 0.44049496994058757 2315 7 0.411104836455948 2315 8 0.25651496120129735 2315 9 0.49669265582349453 2315 10-11 0.5985700799530704 2315 12-13 0.4088444625163561 2315 14-15 0.6313389672260641 2315 16-17 0.7851453633753422 2315 18-19 0.7813422769310705 2315 20-21 0.5250731248878324 2315 22-23 0.8718950214521328 2315 24-25 0.9071711490182324 2315 26-27 0.8624653934293391 2315 28-29 0.8332788981234174 2315 30-31 0.8969518210574989 2315 32-33 0.8390984554541774 2315 34-35 0.8781274721524639 2315 36-37 0.8582922380332363 2315 38-39 0.9225028772099435 2315 40-41 0.895563329940174 2315 42-43 0.9548713324841103 2315 44-45 1.0066544360122336 2315 46-47 1.000450661852824 2315 48-49 0.9185005917095381 2315 50-51 0.9829000227585922 2315 52-53 0.9995089371589714 2315 54-55 0.8670125494435226 2315 56-57 0.8306397153065248 2315 58-59 0.8268446359583734 2315 60-61 0.9381678029955296 2315 62-63 1.0185283783819088 2315 64-65 1.0751684326060271 2315 66-67 1.0874086618579142 2315 68-69 1.0868268625457986 2315 70-71 1.0074983140500393 2315 72-73 1.0917262486986417 2315 74-75 0.5217620848718383 2315 76-77 0.5486800256555107 2315 78-79 0.8699760165200061 2315 80-81 0.8717133094346963 2315 82-83 0.8368983378571713 2315 84-85 0.8907911896178327 2315 86-87 0.8968850889257496 2315 88-89 0.8951328378941952 2315 90-91 0.9748992139168386 2315 92-93 0.9457891069909152 2315 94-95 0.9976289773524663 2315 96-97 1.1548280190036557 2315 98-99 1.1618812137750183 2315 100 1.13521510668998 2316 1 0.25996716927464547 2316 2 0.09859880639938723 2316 3 0.21785626613805675 2316 4 0.012445274533448014 2316 5 0.2454108313134924 2316 6 0.329991741283834 2316 7 0.38787783643948615 2316 8 0.4104246664615019 2316 9 0.5585866405548714 2316 10-11 0.5704478427681892 2316 12-13 0.3491167463433129 2316 14-15 0.5346095895520548 2316 16-17 0.7712159835535033 2316 18-19 0.7495320679633721 2316 20-21 0.7188291087278671 2316 22-23 0.7444656748470635 2316 24-25 0.41159318210680595 2316 26-27 0.7002969700415704 2316 28-29 0.909936089379876 2316 30-31 0.5372153984377874 2316 32-33 0.6820318734996818 2316 34-35 0.8104000852641775 2316 36-37 0.8098674016769891 2316 38-39 0.7313816457564215 2316 40-41 0.7413813099065862 2316 42-43 0.7806403312507584 2316 44-45 0.595138980006304 2316 46-47 0.6831395804398426 2316 48-49 0.8237897824648925 2316 50-51 0.838637885639919 2316 52-53 0.7937065762981561 2316 54-55 0.7203295725343253 2316 56-57 0.8333068186862747 2316 58-59 0.6661475633737339 2316 60-61 0.828568196295187 2316 62-63 0.8173712793706684 2316 64-65 0.8714684396085843 2316 66-67 0.8423727571368502 2316 68-69 0.8471184226832946 2316 70-71 0.8228218425410461 2316 72-73 0.8521757231292355 2316 74-75 0.8795309269906042 2316 76-77 0.7624551587046717 2316 78-79 0.5396876369571757 2316 80-81 0.51061502743039 2316 82-83 0.4049234321703423 2316 84-85 0.558884740014225 2316 86-87 0.6815897308967749 2316 88-89 0.6438428529760785 2316 90-91 0.4688259446873779 2316 92-93 0.3006595533975016 2316 94-95 0.3641917957190479 2316 96-97 0.6200129162355772 2316 98-99 0.8056119518670037 2316 100 0.3984098012118231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 6.0 12 34.0 13 168.0 14 654.0 15 1979.0 16 4707.0 17 9578.0 18 16514.0 19 24967.0 20 35997.0 21 51101.0 22 63389.0 23 66062.0 24 67237.0 25 72921.0 26 85891.0 27 106540.0 28 132269.0 29 162675.0 30 201869.0 31 251329.0 32 321774.0 33 423535.0 34 610292.0 35 855140.0 36 1344209.0 37 1974777.0 38 1596448.0 39 258104.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13920520168026 20.694796648540667 13.666578375582944 23.499419774196127 2 15.474026416823069 19.86686098742956 41.0222297277816 23.63688286796577 3 18.959901091372405 25.656976714917345 29.230732794306025 26.152389399404225 4 12.581879943941718 16.2458117950153 38.49680709278058 32.67550116826241 5 13.173217784331412 38.122063278272734 34.5321061743752 14.172612763020659 6 31.068999888789577 37.37919596237518 17.404431117499993 14.147373031335253 7 27.90023370425942 31.960390411741002 22.16175639059755 17.977619493402024 8 26.524097650988743 35.91634413355868 19.256427893770706 18.303130321681866 9 26.318090661450018 15.636304675236639 21.056449828210702 36.989154835102646 10-11 26.04439008991723 26.237527529564996 26.93827073199782 20.77981164851995 12-13 25.863737240404582 23.587412946656634 27.909139701473208 22.63971011146558 14-15 23.078844355013235 25.02802718320702 25.499213373919094 26.39391508786065 16-17 22.05665428110714 27.856405006251894 26.613594741464148 23.473345971176816 18-19 21.656238973349314 27.523841592423416 28.897278868600996 21.922640565626278 20-21 22.976047308827603 26.650928635737426 28.502004590977286 21.87101946445768 22-23 23.101814285916962 26.4059937841154 28.26888849530468 22.22330343466296 24-25 22.54106285278333 26.34061956458811 28.93879936449516 22.1795182181334 26-27 22.494211905885482 26.6450438233852 28.192273132482608 22.66847113824671 28-29 22.000857878443043 27.01726145704784 28.595074738855075 22.386805925654045 30-31 22.785167498850537 26.660393179347423 28.45700987836837 22.097429443433665 32-33 22.857497433003186 26.86522800413008 27.902222625023015 22.37505193784372 34-35 22.838103874838033 26.773860699562434 28.45330471000801 21.934730715591524 36-37 22.62064311712593 26.459018273437056 28.20644673656251 22.713891872874505 38-39 22.68288475155746 26.611760161932896 28.604885166403214 22.10046992010643 40-41 22.458254029003218 25.97783311680176 28.980150605287257 22.58376224890776 42-43 22.481877379670127 26.143094045410038 28.4208383761345 22.954190198785334 44-45 22.49706064259073 26.78183989184309 28.31452239947684 22.40657706608934 46-47 22.50267821386952 26.630323177148107 27.809383838871053 23.05761477011132 48-49 23.103430245137485 26.609128441369446 27.796056715253574 22.491384598239495 50-51 22.80503563790121 26.550096227883007 27.71543826168011 22.92942987253567 52-53 22.42487232917392 26.969249437604137 27.488068522727005 23.117809710494935 54-55 22.36687057455797 26.880907264247234 27.51144264260966 23.24077951858514 56-57 23.07960610015365 26.58725566253769 27.185764347838575 23.14737388947008 58-59 22.29849875277479 26.94162642744353 27.789007254502597 22.970867565279082 60-61 22.48763036275535 27.381439073694008 27.25259095028723 22.878339613263403 62-63 22.724981290150662 26.90157114679792 26.976667979779062 23.39677958327236 64-65 22.170826627196313 26.93841219582599 27.281137669105103 23.60962350787259 66-67 22.473926503016557 26.691536702497938 27.290160002852932 23.544376791632576 68-69 22.176223608101957 26.756434657424077 27.446440581916846 23.62090115255712 70-71 22.143235562324296 26.405189717181983 27.57858606329836 23.872988657195364 72-73 22.535189825225732 26.57422668062933 27.575096113513105 23.315487380631833 74-75 22.68447895397068 26.832456987721244 27.24020377610724 23.242860282200837 76-77 22.50589365801424 26.694905942963537 27.002218519602838 23.796981879419384 78-79 22.320944890539764 26.739256807864702 27.113891053566913 23.825907248028617 80-81 22.02695823085759 27.12095254543825 27.391390681763482 23.46069854194068 82-83 22.75273095746701 26.36083170773275 28.1009253876518 22.785511947148436 84-85 22.878327120247675 26.632879614954675 27.188874942118996 23.29991832267865 86-87 21.912678097470568 27.175885399426075 27.516526782428603 23.394909720674754 88-89 22.48330634877981 26.33862639711042 27.445523584873797 23.732543669235973 90-91 22.381105499612914 26.33181632478551 27.530889155046808 23.756189020554768 92-93 22.583619278588692 26.497612104113166 27.387167634245852 23.53160098305229 94-95 21.773982915107922 26.441006029574872 27.88157007532034 23.90344097999687 96-97 21.960435359899506 26.417359932799755 27.57414186594403 24.048062841356707 98-99 21.52610297912682 26.788572426488848 27.44978426902206 24.235540325362276 100 21.98064580812541 26.36015204641417 27.035793077337566 24.623409068122857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1173.0 1 832.0 2 850.0 3 1155.0 4 883.5 5 768.5 6 1158.5 7 2187.0 8 3456.0 9 4100.5 10 3828.0 11 3484.5 12 3823.5 13 4455.5 14 5239.0 15 6319.5 16 7432.0 17 8416.5 18 9549.5 19 10654.0 20 11671.0 21 12880.0 22 14441.0 23 16722.0 24 19534.0 25 23121.5 26 28512.0 27 34588.5 28 42785.5 29 56906.0 30 76499.0 31 95831.0 32 120630.0 33 147565.5 34 169321.0 35 191759.5 36 214216.5 37 246422.0 38 263110.5 39 257737.5 40 248973.0 41 239181.5 42 241452.0 43 255826.0 44 277500.5 45 314987.5 46 348422.0 47 371407.5 48 392351.0 49 391920.0 50 379063.0 51 383266.5 52 382745.5 53 363420.0 54 344065.0 55 306736.5 56 269953.0 57 228266.0 58 186674.5 59 171712.5 60 142856.0 61 103540.5 62 78258.5 63 62573.0 64 45120.5 65 26081.0 66 16533.5 67 11600.0 68 8519.0 69 6306.5 70 4738.5 71 3572.5 72 2663.0 73 2076.5 74 1602.0 75 1047.5 76 649.0 77 393.5 78 248.5 79 157.5 80 99.5 81 64.5 82 48.5 83 26.0 84 15.5 85 13.0 86 8.5 87 6.0 88 2.5 89 3.0 90 3.0 91 2.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04501047345892331 2 0.0 3 0.0 4 0.03264231844898531 5 0.0 6 0.0 7 9.381949221229566E-4 8 0.0 9 0.011372753080368524 10-11 4.462146580828696E-4 12-13 1.1441401489304348E-5 14-15 1.4301751861630436E-4 16-17 4.176111543596087E-4 18-19 0.0 20-21 1.1441401489304348E-5 22-23 0.0 24-25 5.720700744652175E-4 26-27 5.549079722312609E-4 28-29 2.517108327646957E-4 30-31 0.002660125846263261 32-33 0.01575480985077209 34-35 0.026612699864121913 36-37 0.032104572578988 38-39 0.02288852367935335 40-41 0.0014072923831844348 42-43 6.864840893582609E-5 44-45 0.011818967738451392 46-47 0.018552232514907 48-49 0.0036269242721094783 50-51 0.01186473334440861 52-53 0.011607301810899261 54-55 0.010846448611860524 56-57 5.434665707419566E-4 58-59 0.009015824373571827 60-61 0.005045658056783218 62-63 0.0015903548070133047 64-65 0.03622347711513757 66-67 0.060782445411929356 68-69 0.06021609603820879 70-71 0.052699095259735824 72-73 0.04614889290710909 74-75 0.03601753188833009 76-77 0.03237916621473131 78-79 0.01599507928204748 80-81 0.0012757162660574349 82-83 0.039095268888952955 84-85 0.052550357040374876 86-87 0.05776191541875301 88-89 0.07069641980241156 90-91 0.04982730348592044 92-93 0.04029089534458526 94-95 0.013506574458123784 96-97 0.0038729144041295223 98-99 0.022768388963715653 100 0.03267664265345322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8740188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.406813627188704 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2648800306063 46.97041340742765 2 14.610917228519426 18.23641576804985 3 4.972745568009131 9.309996176345233 4 2.044507439068815 5.103647788374736 5 1.0486297470612365 3.2720820594388313 6 0.5964375421261114 2.233305991903365 7 0.3571630344027457 1.5602584845745335 8 0.23358102177039136 1.1661637833978495 9 0.16539760775740475 0.9289743913529297 >10 0.6631177614470115 6.862985636295854 >50 0.026995289659524413 1.1610403797036268 >100 0.014296905859588802 1.5963468139991717 >500 8.793139253748439E-4 0.39100223912344456 >1k 3.8021876903332957E-4 0.4045220304950973 >5k 0.0 0.0 >10k+ 7.129101797711908E-5 0.8028450495177065 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22929 0.2623398947482594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19067 0.218153202196566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12140 0.1388986140801548 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1441401489304348E-5 0.0 4 0.0 0.0 0.0 1.1441401489304348E-5 0.0 5 0.0 4.576560595721739E-5 0.0 1.1441401489304348E-5 1.1441401489304348E-5 6 0.0 4.576560595721739E-5 0.0 1.1441401489304348E-5 1.1441401489304348E-5 7 0.0 4.576560595721739E-5 0.0 1.1441401489304348E-5 1.1441401489304348E-5 8 0.0 4.576560595721739E-5 0.0 3.4324204467913045E-5 1.1441401489304348E-5 9 0.0 4.576560595721739E-5 0.0 3.4324204467913045E-5 1.1441401489304348E-5 10-11 0.0 4.576560595721739E-5 0.0 3.4324204467913045E-5 1.7162102233956522E-5 12-13 0.0 4.576560595721739E-5 0.0 3.4324204467913045E-5 2.2882802978608697E-5 14-15 0.0 4.576560595721739E-5 0.0 3.4324204467913045E-5 2.2882802978608697E-5 16-17 0.0 5.720700744652175E-5 0.0 5.720700744652175E-5 2.2882802978608697E-5 18-19 0.0 6.864840893582609E-5 0.0 9.153121191443479E-5 3.4324204467913045E-5 20-21 0.0 1.144140148930435E-4 0.0 1.372968178716522E-4 3.4324204467913045E-5 22-23 0.0 1.144140148930435E-4 0.0 2.6315223425400005E-4 5.1486306701869564E-5 24-25 0.0 1.144140148930435E-4 0.0 7.150875930815218E-4 6.864840893582609E-5 26-27 0.0 1.144140148930435E-4 0.0 0.0013500853757379132 6.864840893582609E-5 28-29 0.0 1.144140148930435E-4 0.0 0.003941562813065349 6.864840893582609E-5 30-31 0.0 1.144140148930435E-4 0.0 0.014742245818968654 7.436910968047826E-5 32-33 0.0 1.144140148930435E-4 0.0 0.04126341447117614 8.008981042513044E-5 34-35 0.0 1.144140148930435E-4 0.0 0.08114814006289109 8.008981042513044E-5 36-37 0.0 1.144140148930435E-4 0.0 0.14670164989586038 8.008981042513044E-5 38-39 0.0 1.2013471563769567E-4 0.0 0.24283802590974013 8.008981042513044E-5 40-41 0.0 1.2585541638234784E-4 0.0 0.34670878933038973 8.008981042513044E-5 42-43 0.0 1.2585541638234784E-4 0.0 0.4487260457097719 8.008981042513044E-5 44-45 0.0 1.31576117127E-4 0.0 0.5662349597056723 8.008981042513044E-5 46-47 0.0 1.3729681787165218E-4 0.0 0.7072673951635824 8.581051116978262E-5 48-49 0.0 1.3729681787165218E-4 0.0 0.8610341104790882 1.0297261340373914E-4 50-51 0.0 1.3729681787165218E-4 0.0 1.0178385178900042 1.0297261340373914E-4 52-53 0.0 1.3729681787165218E-4 0.0 1.1709015870139177 1.144140148930435E-4 54-55 0.0 2.8031433648795657E-4 0.0 1.3343019623834178 1.2585541638234784E-4 56-57 0.0 3.3180064318982615E-4 0.0 1.5240919302879985 1.2585541638234784E-4 58-59 0.0 3.6612484765773915E-4 0.0 1.7441043602265762 1.31576117127E-4 60-61 0.0 3.6612484765773915E-4 0.0 1.966342142754824 1.3729681787165218E-4 62-63 0.0 3.6612484765773915E-4 0.0 2.192538650198371 1.3729681787165218E-4 64-65 0.0 3.6612484765773915E-4 0.0 2.4199422254990397 1.3729681787165218E-4 66-67 0.0 4.1189045361495657E-4 0.0 2.676652950714561 1.4873821936095655E-4 68-69 0.0 4.2333185510426093E-4 0.0 2.957276205042729 1.4873821936095655E-4 70-71 0.0 4.3477325659356525E-4 0.0 3.2601815887713173 1.601796208502609E-4 72-73 0.0 4.462146580828696E-4 0.0 3.564253995451814 2.517108327646957E-4 74-75 0.0 4.462146580828696E-4 0.0 3.8588986873051243 2.517108327646957E-4 76-77 0.0 4.462146580828696E-4 0.0 4.189915594492933 2.5743153350934783E-4 78-79 0.0 4.462146580828696E-4 0.0 4.546177954066892 2.6315223425400005E-4 80-81 0.0 4.690974610614783E-4 0.0 4.925065685085951 2.6315223425400005E-4 82-83 0.0 4.7481816180613046E-4 0.0 5.314593919490061 2.7459363574330436E-4 84-85 0.0 4.8053886255078267E-4 0.0 5.692880976930931 2.7459363574330436E-4 86-87 0.0 5.148630670186957E-4 0.0 6.085858793884068 2.7459363574330436E-4 88 0.0 5.148630670186957E-4 0.0 6.40234512118046 2.7459363574330436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15630 0.0 44.87847 1 GTATCAA 26450 0.0 38.00233 1 GTACTAG 7005 0.0 36.24214 1 CAACGCA 30780 0.0 31.279907 5 TACCTGG 5530 0.0 31.18837 2 ATCAACG 31105 0.0 30.976871 3 TATCAAC 34085 0.0 29.284847 2 AACGCAG 34670 0.0 28.420826 6 GTGGTAT 5505 0.0 27.585028 1 TCAACGC 36070 0.0 26.999609 4 GGGCACC 8240 0.0 26.756336 94 GTACATG 36365 0.0 26.735924 1 GCATCGT 8675 0.0 26.486769 8 TACATGG 36485 0.0 26.057531 2 CTGTGCG 9395 0.0 26.057367 9 ACGCAGA 37785 0.0 25.530636 7 ACATGGG 36010 0.0 25.23031 3 CGCAGAG 38350 0.0 25.1176 8 TAGTACT 9960 0.0 24.963772 4 ACCTGGG 6850 0.0 24.701643 3 >>END_MODULE