FastQCFastQC Report
Mon 6 Jun 2016
ERR1211216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386210
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109670.3238724119295614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83680.2471199364481234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56530.16694180219183097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG30200.048.776292
GGTATCA55800.047.3987731
GTATCAA93950.040.324041
GTACCTG38500.038.8715021
ACCTGGG38800.037.1943743
CAACGCA112400.032.788415
ATCAACG112250.032.748473
TATCAAC117700.031.987842
AACGCAG122950.030.5871126
TCAACGC121400.030.357644
TATCACG2850.029.7234382
CCTGGGG36950.029.5150934
GTACTAG14950.028.9608691
GTACATG100200.028.0395851
ATAACGC4300.027.330163
ACGCAGA135700.027.0896847
TACATGG102950.026.833862
CGCAGAG136450.026.698058
TAACGCC6800.026.2691084
GTGGTAT22400.026.2619481
Produced by FastQC (version 0.11.5)