##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3386210 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.048890352340816 31.0 31.0 34.0 28.0 34.0 2 31.042913463724933 31.0 31.0 34.0 27.0 34.0 3 31.10212538501747 31.0 31.0 34.0 27.0 34.0 4 34.688794552021285 37.0 35.0 37.0 31.0 37.0 5 34.743923737748105 37.0 35.0 37.0 32.0 37.0 6 34.80345637157767 37.0 35.0 37.0 31.0 37.0 7 34.71340229932579 37.0 35.0 37.0 31.0 37.0 8 34.71203174050044 37.0 35.0 37.0 31.0 37.0 9 36.22468423399612 39.0 35.0 39.0 31.0 39.0 10-11 36.15770758458572 39.0 35.0 39.0 30.0 39.0 12-13 36.047672619240984 39.0 35.0 39.0 30.0 39.0 14-15 37.182057226220465 40.0 36.0 41.0 31.0 41.0 16-17 37.15014780536352 40.0 36.0 41.0 31.0 41.0 18-19 37.15899132068006 40.0 36.5 41.0 31.0 41.0 20-21 37.11789389907891 40.0 36.0 41.0 30.0 41.0 22-23 36.999841415623955 40.0 36.0 41.0 30.0 41.0 24-25 36.902280277950865 40.0 36.0 41.0 30.0 41.0 26-27 36.74805711990692 39.0 36.0 41.0 30.0 41.0 28-29 36.60772353161795 39.0 36.0 41.0 29.0 41.0 30-31 36.438726481818904 39.0 35.0 41.0 29.0 41.0 32-33 36.22858505526828 39.0 35.0 40.5 28.5 41.0 34-35 36.05081418458985 39.0 35.0 40.0 27.5 41.0 36-37 35.92451531358067 39.0 35.0 40.0 27.0 41.0 38-39 35.78082708987334 38.0 35.0 40.0 27.0 41.0 40-41 35.615253336325864 38.0 34.0 40.0 26.0 41.0 42-43 35.40120178606761 38.0 34.0 40.0 25.5 41.0 44-45 35.199110952953305 38.0 34.0 40.0 25.0 41.0 46-47 34.98060279781821 38.0 33.0 40.0 24.0 41.0 48-49 34.780884528720904 38.0 33.0 40.0 24.0 41.0 50-51 34.14169425404803 37.5 32.5 39.5 23.0 40.5 52-53 34.27964892903866 37.5 33.0 39.5 23.5 40.5 54-55 34.77813041719208 38.0 33.0 40.0 24.0 41.0 56-57 34.75699174002794 38.0 33.0 40.0 24.0 41.0 58-59 34.563800827473784 38.0 33.0 40.0 23.0 41.0 60-61 34.3432200011222 37.0 33.0 40.0 23.0 41.0 62-63 34.04848326004589 37.0 32.5 40.0 23.0 41.0 64-65 33.80164121540011 36.0 32.0 39.5 22.0 41.0 66-67 33.6270844100041 36.0 32.0 39.0 22.0 41.0 68-69 33.32714583560973 35.5 32.0 39.0 22.0 41.0 70-71 33.00015932266457 35.0 31.5 39.0 21.5 40.5 72-73 32.64361011278096 35.0 31.0 37.5 20.5 40.0 74-75 32.30592195994933 35.0 31.0 37.0 20.0 39.5 76-77 31.96061629373252 35.0 31.0 36.5 20.0 39.0 78-79 31.61658491351688 35.0 30.0 36.0 20.0 39.0 80-81 31.31086568759764 34.5 30.0 36.0 19.0 37.5 82-83 31.01596608007182 34.0 30.0 35.0 18.5 37.0 84-85 30.75652794717398 34.0 29.5 35.0 18.0 37.0 86-87 30.52347137360057 34.0 29.0 35.0 18.0 36.0 88-89 30.293823773481265 34.0 29.0 35.0 17.5 36.0 90-91 30.062041928882145 34.0 29.0 35.0 17.0 36.0 92-93 29.86257054346895 34.0 29.0 35.0 16.0 35.0 94-95 29.680290649428123 34.0 29.0 35.0 15.0 35.0 96-97 29.496446764967324 34.0 28.5 35.0 14.5 35.0 98-99 29.25451862111328 34.0 27.0 35.0 11.5 35.0 100 28.131423331689412 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4665531756404775 1101 2 -0.4409288458255105 1101 3 -0.45085680432661945 1101 4 -0.2628148463047779 1101 5 -0.21267793677569813 1101 6 -0.2802274351321117 1101 7 -0.20687438674374903 1101 8 -0.19988286432671032 1101 9 -0.34602414824720285 1101 10-11 -0.4436045317776589 1101 12-13 -0.4612556357192972 1101 14-15 -0.5200494864310556 1101 16-17 -0.6088357669568438 1101 18-19 -0.5990636876084992 1101 20-21 -0.6040457876454397 1101 22-23 -0.6673660846968303 1101 24-25 -0.6971446128562206 1101 26-27 -0.6781513156680603 1101 28-29 -0.6849540539857983 1101 30-31 -0.7777593649272205 1101 32-33 -0.8015443782569633 1101 34-35 -0.8261539683629664 1101 36-37 -0.804220691323259 1101 38-39 -0.9627426548024403 1101 40-41 -0.8825468302720125 1101 42-43 -0.8755498823938481 1101 44-45 -0.8533900694642327 1101 46-47 -1.0883228124720716 1101 48-49 -0.9357468883247506 1101 50-51 -1.13467324670939 1101 52-53 -1.0666035430106788 1101 54-55 -0.9556429271290838 1101 56-57 -0.8871754929570201 1101 58-59 -0.8604149605385842 1101 60-61 -0.8355357371387484 1101 62-63 -0.8852958865185343 1101 64-65 -0.6673811511737426 1101 66-67 -0.7227755331697523 1101 68-69 -0.8492108304753003 1101 70-71 -0.9538861560262575 1101 72-73 -0.9417363149159712 1101 74-75 -0.9011889764838372 1101 76-77 -0.9522786433160455 1101 78-79 -0.9596239630115555 1101 80-81 -1.1918687540562338 1101 82-83 -1.197733211118134 1101 84-85 -1.2863686547865107 1101 86-87 -1.097986509071859 1101 88-89 -1.1394262836808373 1101 90-91 -1.2117304574504182 1101 92-93 -1.3801584856807985 1101 94-95 -1.4869482229670083 1101 96-97 -1.4943375516661632 1101 98-99 -1.5583783558841908 1101 100 -1.4952876223590934 1102 1 -0.3381791460245651 1102 2 -0.25899501241193335 1102 3 -0.36996420379590234 1102 4 -0.3212414469954794 1102 5 -0.3367208304024629 1102 6 -0.24293169243317436 1102 7 -0.32018552768846575 1102 8 -0.31445761005036843 1102 9 -0.45111015206294525 1102 10-11 -0.4487097313736683 1102 12-13 -0.5326099603410768 1102 14-15 -0.6253031688017003 1102 16-17 -0.6187408871250284 1102 18-19 -0.6886517273041974 1102 20-21 -0.7527111443888828 1102 22-23 -0.7746546715210485 1102 24-25 -0.7050312495799247 1102 26-27 -0.6723615821913356 1102 28-29 -0.7630379574298232 1102 30-31 -0.8296976618203686 1102 32-33 -0.9140560924520642 1102 34-35 -0.9165151503547904 1102 36-37 -0.83443681131304 1102 38-39 -0.965195129800783 1102 40-41 -1.1524300167411852 1102 42-43 -1.0056515322337631 1102 44-45 -1.0467010608314382 1102 46-47 -1.1289248684474416 1102 48-49 -1.0720853783246298 1102 50-51 -1.1204023059123855 1102 52-53 -0.8403180814892792 1102 54-55 -0.843802469101071 1102 56-57 -0.8280815923159466 1102 58-59 -0.7324335176879302 1102 60-61 -0.6812218784357285 1102 62-63 -0.8028535871263642 1102 64-65 -0.8404490487404672 1102 66-67 -0.8866961157503113 1102 68-69 -0.8271093987150522 1102 70-71 -0.7932496668748499 1102 72-73 -0.7947721313587905 1102 74-75 -0.8570335333691332 1102 76-77 -1.0026351580560409 1102 78-79 -1.071429898984146 1102 80-81 -1.230313479638955 1102 82-83 -1.2406711691857062 1102 84-85 -1.2629178821536478 1102 86-87 -1.2924378121629303 1102 88-89 -1.3696026030465944 1102 90-91 -1.438237141939208 1102 92-93 -1.3617106471516713 1102 94-95 -1.4628388757384307 1102 96-97 -1.5604755774554917 1102 98-99 -1.6696497148478855 1102 100 -1.7721498460823888 1103 1 -0.5966607341289283 1103 2 -0.5089579771680341 1103 3 -0.6174148755407423 1103 4 -0.4048258544543373 1103 5 -0.4028696036669075 1103 6 -0.37214838254170246 1103 7 -0.48452642241858257 1103 8 -0.4977185487924132 1103 9 -0.6579323336498533 1103 10-11 -0.7043513990438086 1103 12-13 -0.6139017517171936 1103 14-15 -0.8237335055079313 1103 16-17 -0.8665736012042586 1103 18-19 -0.8434196839146182 1103 20-21 -0.8366406106634301 1103 22-23 -0.7743307683125593 1103 24-25 -0.890265090937497 1103 26-27 -0.9031275144842894 1103 28-29 -0.9467491123211502 1103 30-31 -0.8535953253723463 1103 32-33 -0.9633666058681314 1103 34-35 -0.9309850167010083 1103 36-37 -0.980869872489059 1103 38-39 -0.964499063771278 1103 40-41 -1.0060609913639738 1103 42-43 -1.229562892618965 1103 44-45 -1.0763391871874148 1103 46-47 -1.2456149562851095 1103 48-49 -1.308382079456834 1103 50-51 -1.39096943549891 1103 52-53 -1.2371614786846123 1103 54-55 -0.9941177652361333 1103 56-57 -0.9672035032352184 1103 58-59 -0.8744258151848001 1103 60-61 -0.9280723309484813 1103 62-63 -0.8981597527289367 1103 64-65 -0.7798412341798624 1103 66-67 -0.9592363134729922 1103 68-69 -0.8916066380376293 1103 70-71 -0.9854395649844854 1103 72-73 -0.9083830179825299 1103 74-75 -0.880522567833431 1103 76-77 -0.8891704985754529 1103 78-79 -0.960107335251454 1103 80-81 -0.9879376190867859 1103 82-83 -0.8262889977452161 1103 84-85 -0.7496359367729788 1103 86-87 -0.9158719351538736 1103 88-89 -0.9229246126989317 1103 90-91 -1.1265464085710342 1103 92-93 -1.410737189214398 1103 94-95 -1.4797790859579223 1103 96-97 -1.3482198186819652 1103 98-99 -1.3547302427097705 1103 100 -1.2759670624638026 1104 1 -0.423714295817593 1104 2 -0.39144285650575483 1104 3 -0.3841166924443691 1104 4 -0.23992639625281953 1104 5 -0.3328800275302797 1104 6 -0.2165497982477973 1104 7 -0.362912662918923 1104 8 -0.4100138592659732 1104 9 -0.46599870699385093 1104 10-11 -0.5262481785548587 1104 12-13 -0.49962621022173437 1104 14-15 -0.7342249421829621 1104 16-17 -0.6845735527211687 1104 18-19 -0.8111981283348229 1104 20-21 -0.708621932554756 1104 22-23 -0.632591680036974 1104 24-25 -0.637977295342175 1104 26-27 -0.8076640161872533 1104 28-29 -0.7142339310550128 1104 30-31 -0.621509219412566 1104 32-33 -0.7326728735190002 1104 34-35 -0.9205905461969124 1104 36-37 -0.8597721548303028 1104 38-39 -0.9938795105429179 1104 40-41 -0.8429375291868411 1104 42-43 -0.8726843548688166 1104 44-45 -0.855698725628443 1104 46-47 -0.8407430122345971 1104 48-49 -0.8668293480410014 1104 50-51 -0.9727091904169143 1104 52-53 -0.7121635998196041 1104 54-55 -0.5158254607138701 1104 56-57 -0.6541394328377734 1104 58-59 -0.7074458265783292 1104 60-61 -0.6631825693640536 1104 62-63 -0.7089623902087894 1104 64-65 -0.6934513453273325 1104 66-67 -0.7402863965002737 1104 68-69 -0.8818121457161396 1104 70-71 -0.7526428788033783 1104 72-73 -0.66129200311034 1104 74-75 -0.6325564332686291 1104 76-77 -0.6794069142209445 1104 78-79 -0.7937964132243778 1104 80-81 -0.8311212002898962 1104 82-83 -0.8731345669972441 1104 84-85 -0.9036512453072127 1104 86-87 -0.6947217951589622 1104 88-89 -1.0027292864683695 1104 90-91 -1.0377517586795157 1104 92-93 -0.9888413187611995 1104 94-95 -1.0714230261782802 1104 96-97 -1.0291135437586298 1104 98-99 -1.0206636027087974 1104 100 -1.1056247718804286 1105 1 -0.5524659775746201 1105 2 -0.5126587083968559 1105 3 -0.5944970941959937 1105 4 -0.33468139217504245 1105 5 -0.3823327747031158 1105 6 -0.374654178867722 1105 7 -0.45092556634581626 1105 8 -0.495061156247381 1105 9 -0.6348897083600349 1105 10-11 -0.7348668261884441 1105 12-13 -0.6853347818580886 1105 14-15 -0.8452824621692443 1105 16-17 -0.8925857289017785 1105 18-19 -0.9002548899071314 1105 20-21 -0.869309595860507 1105 22-23 -0.8541506363346727 1105 24-25 -0.9392128348290569 1105 26-27 -0.8728751513667561 1105 28-29 -0.8366902573184731 1105 30-31 -0.9738735490213557 1105 32-33 -1.0112219652403454 1105 34-35 -1.0073047955488832 1105 36-37 -1.025621968083847 1105 38-39 -0.9177851347539772 1105 40-41 -0.9168376990507952 1105 42-43 -0.8936256712884827 1105 44-45 -1.0351228504654628 1105 46-47 -1.1294119957001243 1105 48-49 -1.1878942963286008 1105 50-51 -1.0800411315930774 1105 52-53 -1.016633567145469 1105 54-55 -0.8064951506472156 1105 56-57 -0.8094854722025744 1105 58-59 -0.8194944793545886 1105 60-61 -0.7997505902671875 1105 62-63 -0.832741525512958 1105 64-65 -0.85803367220376 1105 66-67 -0.9245430728808728 1105 68-69 -0.7826936721021838 1105 70-71 -0.9311636598658808 1105 72-73 -0.8831823759279551 1105 74-75 -0.9057410447373009 1105 76-77 -0.9281173507245519 1105 78-79 -1.0272733776983962 1105 80-81 -0.9847763920491559 1105 82-83 -1.0270915659635165 1105 84-85 -0.8228702866927193 1105 86-87 -1.0185471625482023 1105 88-89 -1.1700398258253806 1105 90-91 -1.1013234729548422 1105 92-93 -1.1631671874309113 1105 94-95 -1.246338743528817 1105 96-97 -1.29568281136968 1105 98-99 -1.2469096612936816 1105 100 -1.2372386872185714 1106 1 -0.5614181424174269 1106 2 -0.5736350705407318 1106 3 -0.6274170308270897 1106 4 -0.5620340673152953 1106 5 -0.491434996274684 1106 6 -0.38273998831135714 1106 7 -0.46821293805253816 1106 8 -0.3501643921396962 1106 9 -0.5807621026275527 1106 10-11 -0.5672652241890859 1106 12-13 -0.6590445292713127 1106 14-15 -0.7130017044611918 1106 16-17 -0.8169623660625192 1106 18-19 -0.8642504248044673 1106 20-21 -0.6843764416889826 1106 22-23 -0.8025509897783891 1106 24-25 -0.7757691636153723 1106 26-27 -0.8104633162304751 1106 28-29 -0.8974713365766576 1106 30-31 -0.879818043717087 1106 32-33 -0.9078375847341249 1106 34-35 -0.934436829784822 1106 36-37 -0.9251474855704274 1106 38-39 -0.9806636097175172 1106 40-41 -0.9661104206197493 1106 42-43 -0.9684218921594479 1106 44-45 -1.0872215532851683 1106 46-47 -0.9928397201185959 1106 48-49 -1.0360170186818252 1106 50-51 -1.019917269436128 1106 52-53 -0.8810107794365294 1106 54-55 -0.7929756668005368 1106 56-57 -0.8935784132621762 1106 58-59 -0.9462649657579831 1106 60-61 -0.9077990162400695 1106 62-63 -0.9761097500856621 1106 64-65 -0.9088783295045175 1106 66-67 -0.7774304885066456 1106 68-69 -0.9658522878157925 1106 70-71 -0.9337914825793519 1106 72-73 -0.904044182569141 1106 74-75 -0.7880691199852592 1106 76-77 -0.791691471951566 1106 78-79 -0.831540844667618 1106 80-81 -0.7937567905621812 1106 82-83 -0.7883264753282049 1106 84-85 -0.9062265380978687 1106 86-87 -0.9261313014996873 1106 88-89 -0.9194270506124518 1106 90-91 -0.9501554355609123 1106 92-93 -0.9833950436065031 1106 94-95 -1.1193416034950445 1106 96-97 -1.103025395587057 1106 98-99 -0.9325185935903875 1106 100 -1.2572919129153952 1107 1 -0.5596193218660197 1107 2 -0.5372690324762637 1107 3 -0.5493801757493664 1107 4 -0.4868915285947679 1107 5 -0.4150287626334901 1107 6 -0.4796959659356901 1107 7 -0.4312369213480878 1107 8 -0.42912394509600205 1107 9 -0.72776881395815 1107 10-11 -0.6457669996241862 1107 12-13 -0.6848319291126046 1107 14-15 -0.9092043085631403 1107 16-17 -0.9027825642419742 1107 18-19 -0.862654098766285 1107 20-21 -1.0176883791294742 1107 22-23 -0.9607421105481038 1107 24-25 -0.8801141606457108 1107 26-27 -0.9823496512020924 1107 28-29 -1.1250888191085124 1107 30-31 -1.0613344874065902 1107 32-33 -1.070926049249401 1107 34-35 -1.1189619241338562 1107 36-37 -1.0140551013014942 1107 38-39 -0.9292039760373783 1107 40-41 -1.0693246479752503 1107 42-43 -1.0795332098365904 1107 44-45 -1.0172976391675022 1107 46-47 -0.818733137261205 1107 48-49 -0.8847991651138827 1107 50-51 -0.847236318401734 1107 52-53 -0.7768115256182853 1107 54-55 -0.7135211544360942 1107 56-57 -0.7229504405458513 1107 58-59 -0.6372030060572769 1107 60-61 -0.7204831766583908 1107 62-63 -0.716636881610718 1107 64-65 -0.8454978893296854 1107 66-67 -0.8573826021501461 1107 68-69 -0.7568831360618375 1107 70-71 -0.6934184112674231 1107 72-73 -0.6746960788942999 1107 74-75 -0.6340084665429515 1107 76-77 -0.7741042926575723 1107 78-79 -0.8212112272398784 1107 80-81 -0.7985647020849456 1107 82-83 -0.7817518860168242 1107 84-85 -0.8155982327015039 1107 86-87 -0.85712646491897 1107 88-89 -0.8263001713371452 1107 90-91 -0.9930318752582501 1107 92-93 -0.9946628187500188 1107 94-95 -0.9420103236455688 1107 96-97 -0.8952093226401097 1107 98-99 -0.9493604717453401 1107 100 -0.9418108428989669 1108 1 -0.7329414239478282 1108 2 -0.6113303074032999 1108 3 -0.6966128270134178 1108 4 -0.5855348074305695 1108 5 -0.49128645960738737 1108 6 -0.511322510907732 1108 7 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1.2432086171348047 2311 72-73 1.2988716254478803 2311 74-75 1.3793297532448108 2311 76-77 1.3616298402866853 2311 78-79 1.4207646296393754 2311 80-81 1.3095898656898974 2311 82-83 1.4328029536420814 2311 84-85 1.3319623205074613 2311 86-87 1.3337802852848668 2311 88-89 1.5173928157705845 2311 90-91 1.6223350915945751 2311 92-93 1.6061503429520307 2311 94-95 1.568517367454902 2311 96-97 1.6592128547619431 2311 98-99 1.7730570894515587 2311 100 1.8370160708644363 2312 1 0.611696339812621 2312 2 0.6495548984716955 2312 3 0.6650714561997546 2312 4 0.30948346477897104 2312 5 0.45236649826450304 2312 6 0.44876608036344123 2312 7 0.3238033023423057 2312 8 0.47631808898034933 2312 9 0.7241683239966505 2312 10-11 0.5976066630135151 2312 12-13 0.6772723764643587 2312 14-15 0.927531394097791 2312 16-17 0.9966934736742559 2312 18-19 0.9103973310742504 2312 20-21 0.8851363960193908 2312 22-23 1.0177749413353752 2312 24-25 0.9887844237411656 2312 26-27 0.9438845242198681 2312 28-29 0.9742696826435662 2312 30-31 1.0238243174169668 2312 32-33 1.0451711499290113 2312 34-35 1.0574762563527074 2312 36-37 1.1181656573886514 2312 38-39 1.0667038506890876 2312 40-41 1.2178031313224196 2312 42-43 1.1858572338456739 2312 44-45 1.207243960652633 2312 46-47 1.1876432301096855 2312 48-49 1.2279456409636325 2312 50-51 1.3350392554629522 2312 52-53 1.1451533192479886 2312 54-55 1.0730547030021071 2312 56-57 1.0074519730583589 2312 58-59 1.0128792585946798 2312 60-61 0.9612533851315774 2312 62-63 1.0200133346643554 2312 64-65 1.1131049246530367 2312 66-67 1.0263672234344767 2312 68-69 0.9411437842286574 2312 70-71 1.094286462065881 2312 72-73 1.0577851908740499 2312 74-75 1.0600659270246737 2312 76-77 1.022119553413308 2312 78-79 1.07195657193353 2312 80-81 1.0381325758774231 2312 82-83 1.013590096085263 2312 84-85 1.0949523441031097 2312 86-87 1.0860187668844006 2312 88-89 1.0555600654290664 2312 90-91 1.1754672065095058 2312 92-93 1.1576098060476134 2312 94-95 1.229210437786481 2312 96-97 1.2337492210325856 2312 98-99 0.9262833799677672 2312 100 1.1317215392586917 2313 1 0.7280404965024054 2313 2 0.7797459848729176 2313 3 0.6781984643918868 2313 4 0.7073358108135679 2313 5 0.43870762477256164 2313 6 0.6051232673413622 2313 7 0.7826056255491878 2313 8 0.7852688049494532 2313 9 0.9126356829095172 2313 10-11 0.9450994029919926 2313 12-13 0.9866116194965855 2313 14-15 1.1453300760194054 2313 16-17 1.1949159401915068 2313 18-19 1.091460031766779 2313 20-21 1.1238296187494043 2313 22-23 1.1840851731645472 2313 24-25 1.1675898480704845 2313 26-27 1.1877729821157956 2313 28-29 1.3755419409244993 2313 30-31 1.2591913393107887 2313 32-33 1.3577520666755234 2313 34-35 1.2868899275901455 2313 36-37 1.280387329116607 2313 38-39 1.3650765151562538 2313 40-41 1.416031108412632 2313 42-43 1.5287795552891694 2313 44-45 1.6106533711404083 2313 46-47 1.4928303514319765 2313 48-49 1.4090426718922657 2313 50-51 1.4352597923929054 2313 52-53 1.4319276844679862 2313 54-55 1.3289794873550207 2313 56-57 1.2535661957078048 2313 58-59 1.3308323822407928 2313 60-61 1.240207202223111 2313 62-63 1.3455592994301284 2313 64-65 1.3125809831770994 2313 66-67 1.3861381216806663 2313 68-69 1.3030736236138267 2313 70-71 1.234569624440546 2313 72-73 1.2885395148369625 2313 74-75 1.223564784879251 2313 76-77 1.0073656277318186 2313 78-79 1.16770426547372 2313 80-81 1.2712010871207617 2313 82-83 1.2664364020251782 2313 84-85 1.3671370873914874 2313 86-87 1.3551785223213741 2313 88-89 1.3402301201902276 2313 90-91 1.526473696824329 2313 92-93 1.5646695594070366 2313 94-95 1.4910358739831686 2313 96-97 1.4842048250699236 2313 98-99 1.6402341301404313 2313 100 1.804409530871471 2314 1 0.675454631172002 2314 2 0.6359834995696616 2314 3 0.589984030385601 2314 4 0.4871944809260498 2314 5 0.5188620384600995 2314 6 0.6573450103466385 2314 7 0.6263987314754758 2314 8 0.6558720689811963 2314 9 0.7612015378941877 2314 10-11 0.7434909494101944 2314 12-13 0.824474354819543 2314 14-15 1.0345317145272404 2314 16-17 1.003540304439042 2314 18-19 0.9159958839001163 2314 20-21 0.9724987300314965 2314 22-23 1.0096119020817795 2314 24-25 0.9887814568017745 2314 26-27 1.058632636010195 2314 28-29 0.9956307809254099 2314 30-31 1.1051386072849638 2314 32-33 0.9895690985871113 2314 34-35 1.0837023316951573 2314 36-37 1.0621036686294048 2314 38-39 1.1224577722412334 2314 40-41 1.2111880926978174 2314 42-43 1.266408896666853 2314 44-45 1.2889670700446771 2314 46-47 1.286879317389129 2314 48-49 1.3220178320384903 2314 50-51 1.2851643861319033 2314 52-53 1.2938233763022353 2314 54-55 1.225682904453329 2314 56-57 1.1821428921469632 2314 58-59 1.1222330418874478 2314 60-61 1.2248496321510345 2314 62-63 1.2259974289022608 2314 64-65 1.1144371422523278 2314 66-67 1.1751298737223124 2314 68-69 1.1496270922276253 2314 70-71 1.1097623888141257 2314 72-73 1.048559060148726 2314 74-75 1.188689734438654 2314 76-77 1.1674745501842985 2314 78-79 1.1091766412016746 2314 80-81 1.1151093857846242 2314 82-83 1.2048618796015376 2314 84-85 1.251578187332008 2314 86-87 1.2527710215110943 2314 88-89 1.1604506447042056 2314 90-91 1.3826314627027436 2314 92-93 1.4800433045735382 2314 94-95 1.4671112589364093 2314 96-97 1.4852001780188893 2314 98-99 1.4718225000526601 2314 100 1.515261874305267 2315 1 0.4094901138964069 2315 2 0.42860966970352976 2315 3 0.46950668315390587 2315 4 0.177202984220159 2315 5 0.2769755732322565 2315 6 0.35598731812071804 2315 7 0.3363903362893552 2315 8 0.3533393029505518 2315 9 0.55823869425042 2315 10-11 0.4794889225054746 2315 12-13 0.5000214623361856 2315 14-15 0.6920681808541858 2315 16-17 0.7373063880552877 2315 18-19 0.5131374605638754 2315 20-21 0.6233044829141576 2315 22-23 0.5509825180174417 2315 24-25 0.7061823929449034 2315 26-27 0.6653335416788764 2315 28-29 0.731487072492186 2315 30-31 0.7136390905877477 2315 32-33 0.7563113327396493 2315 34-35 0.7685365763188017 2315 36-37 0.8564201972962167 2315 38-39 0.8786006785131732 2315 40-41 0.799892140945694 2315 42-43 1.0074634408721508 2315 44-45 0.9187722450300768 2315 46-47 0.9373347102902017 2315 48-49 0.8760267526294498 2315 50-51 0.9121394140267682 2315 52-53 0.9455618909392811 2315 54-55 0.8198329227896508 2315 56-57 0.7770660628005857 2315 58-59 0.728059714267026 2315 60-61 0.6685474707960068 2315 62-63 0.7282509700095474 2315 64-65 0.7708890752172053 2315 66-67 0.7573405956566717 2315 68-69 0.8065364120854213 2315 70-71 0.730299976220941 2315 72-73 0.5317292585232778 2315 74-75 0.6910800021248562 2315 76-77 0.7385651339902672 2315 78-79 0.6521110392305509 2315 80-81 0.4849693763263758 2315 82-83 0.4465974440916227 2315 84-85 0.8053171983048522 2315 86-87 0.8876746294231097 2315 88-89 0.9048589090075829 2315 90-91 0.8167731254925386 2315 92-93 0.9837105078162018 2315 94-95 1.0629280622575834 2315 96-97 0.9410662317372704 2315 98-99 0.3340491655133242 2315 100 0.6801347891573712 2316 1 0.20805644822923597 2316 2 0.18422638483159304 2316 3 0.20471355072076136 2316 4 0.122066667669678 2316 5 -0.033165341927428926 2316 6 0.15187615964801182 2316 7 0.10123913238872007 2316 8 0.19169030069036808 2316 9 0.23093357973061757 2316 10-11 0.15239754788618853 2316 12-13 -0.058869471127870554 2316 14-15 0.031982203312900026 2316 16-17 0.11902921559307345 2316 18-19 0.07632341109128049 2316 20-21 0.07576336180197529 2316 22-23 0.15514893577531552 2316 24-25 0.22189691682388712 2316 26-27 0.26856547155870913 2316 28-29 0.1792024695891783 2316 30-31 0.09607387387840305 2316 32-33 0.2458153464974302 2316 34-35 0.3017674787031126 2316 36-37 0.2761866745838759 2316 38-39 0.38356028972895473 2316 40-41 0.4438389332204693 2316 42-43 0.3661450029766016 2316 44-45 0.36876161164617116 2316 46-47 0.2755930680043406 2316 48-49 0.24665765264874295 2316 50-51 0.2864640899903961 2316 52-53 0.389321644717775 2316 54-55 0.061747338723392886 2316 56-57 -0.11767697723454518 2316 58-59 -0.12329139945664025 2316 60-61 -0.011007861982342604 2316 62-63 0.13980122057569844 2316 64-65 0.1779791864520206 2316 66-67 0.07486069936938833 2316 68-69 0.14089380982954935 2316 70-71 -0.13159705867941085 2316 72-73 -0.2444305661076811 2316 74-75 -0.021605229676481486 2316 76-77 0.16770642038403238 2316 78-79 0.2277716248025179 2316 80-81 0.3027157189906795 2316 82-83 0.35104544586403463 2316 84-85 0.5049240418784287 2316 86-87 0.504640412474263 2316 88-89 0.4920487357113039 2316 90-91 0.33927282664880565 2316 92-93 0.263584940143307 2316 94-95 0.47635663223318403 2316 96-97 0.391784709666549 2316 98-99 0.31065421982323116 2316 100 0.4676961991704225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 17.0 1 244.0 2 77.0 3 75.0 4 62.0 5 41.0 6 69.0 7 499.0 8 1523.0 9 2223.0 10 3300.0 11 5466.0 12 8614.0 13 11207.0 14 12844.0 15 14566.0 16 17491.0 17 20623.0 18 24224.0 19 27790.0 20 31859.0 21 36527.0 22 39740.0 23 41040.0 24 41002.0 25 43138.0 26 46612.0 27 53031.0 28 61080.0 29 71571.0 30 84967.0 31 103840.0 32 128467.0 33 165680.0 34 225115.0 35 305742.0 36 441690.0 37 616604.0 38 555090.0 39 142274.0 40 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.149130575202946 18.843146588800057 12.35571023290544 23.652012603091556 2 16.10131551556098 19.88720772892005 40.69082309024328 23.320653665275685 3 19.500759986602024 25.688403720951115 29.200705579477233 25.61013071296963 4 12.665819551466088 16.037149014298173 38.19482349994447 33.10220793429127 5 13.16451972214518 36.91060930572035 34.57450563563707 15.350365336497408 6 30.549315346560835 36.32142412879279 18.736247615169898 14.393012909476473 7 27.31178264892048 31.969609711370854 22.57594691155087 18.142660728157793 8 26.016645225872903 35.03274029890259 20.580000555471184 18.370613919753325 9 26.422109094862616 16.10607369048319 21.14711182029905 36.32470539435514 10-11 25.34082739246124 25.8900746259507 28.122855772450748 20.646242209137313 12-13 25.48453923791392 23.754048290544503 28.37440269064708 22.38700978089449 14-15 22.841185886951052 25.134842115725633 26.42573171997855 25.598240277344768 16-17 22.112022788421122 27.601586021179834 27.318296220528303 22.968094969870737 18-19 21.660754105231632 27.142134964128097 29.37143719622157 21.8256737344187 20-21 22.704434580107304 26.57451637638813 29.082621569028237 21.638427474476323 22-23 22.656821281746232 26.39598504434928 28.799508469231693 22.147685204672793 24-25 22.14633810605219 26.340259447171228 29.318989576268585 22.194412870508 26-27 22.13397843602473 26.567759781952926 28.869172494817985 22.429089287204356 28-29 21.85026947571824 26.997091023910258 28.969780586967193 22.182858913404313 30-31 22.582864707649346 26.634336688022746 29.02104170918733 21.76175689514058 32-33 22.504016501403523 26.74124455863383 28.6494630338609 22.105275906101745 34-35 22.656941615725103 26.749128245724673 28.804016641977427 21.7899134965728 36-37 22.434235041354682 26.683397291641462 28.64512510085563 22.237242566148222 38-39 22.38168260015085 26.970322233431858 28.672429216473112 21.97556594994418 40-41 22.514904172429812 26.41027477733281 28.86023789075221 22.21458315948517 42-43 22.333305860294576 26.62245279880844 28.516467723462892 22.527773617434093 44-45 22.23305824092517 27.091717624841866 28.55952874052899 22.115695393703973 46-47 22.297931834462258 26.715229218959752 28.160257899859936 22.826581046718054 48-49 22.43582992090997 26.749184260816733 28.278145900800737 22.53683991747256 50-51 22.34912751870162 27.010002211094374 28.054740611452523 22.58612965875148 52-53 22.256005686612355 27.28649205032392 27.82427989666721 22.633222366396517 54-55 22.129212965315453 27.135789214950602 28.025178232318275 22.70981958741567 56-57 22.488515708714235 27.06184721822975 27.700104672568077 22.74953240048794 58-59 22.193120384699704 27.18265258669541 28.03137739440169 22.592849634203198 60-61 22.378712422720863 27.375206901099684 27.850634501628846 22.395446174550607 62-63 22.49313795458466 27.12160678796014 27.710286392176975 22.674968865278228 64-65 22.165728276379312 26.969559880407118 27.8507620479432 23.01394979527037 66-67 22.167971043499005 26.934819069873583 27.744859608088735 23.152350278538673 68-69 21.94806194301884 27.016691136206482 27.90130229948678 23.133944621287895 70-71 22.011095362357164 26.71370611840322 27.99848437734277 23.276714141896846 72-73 22.135222621347232 26.959994989106622 27.887276171724736 23.017506217821406 74-75 22.335798140996214 27.014280169228073 27.714862754759316 22.935058935016396 76-77 22.191913014312757 26.84565968778693 27.649992549054765 23.312434748845547 78-79 22.0095522065349 26.913228930257137 27.748644550488176 23.328574312719788 80-81 21.969975801515716 27.056620081433568 27.781038573423732 23.192365543626984 82-83 22.327807321486937 26.77770184879604 28.201668097534956 22.692822732182073 84-85 22.419716243622364 27.018954257342486 27.590436583375087 22.97089291566006 86-87 21.887658974194633 27.15834079365187 27.86962838605177 23.084371846101725 88-89 22.130789826148483 26.612897151624626 27.915517115846217 23.34079590638067 90-91 22.32022911992287 26.59970119415999 27.935938698132844 23.144130987784287 92-93 22.320919046713225 26.647345095625237 27.94087327514807 23.090862582513466 94-95 21.87020551202547 26.672358759590452 28.083433616963205 23.37400211142087 96-97 21.9184204447506 26.632116898714997 27.960454994715477 23.489007661818928 98-99 21.827967852309918 26.696528558739068 27.733408837335844 23.742094751615166 100 22.107062960261636 26.49065762486755 27.578698480754422 23.823580934116396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1079.0 1 776.5 2 1332.0 3 1998.0 4 1314.0 5 928.0 6 1279.0 7 2107.0 8 3128.0 9 3579.5 10 3187.0 11 2702.0 12 2701.5 13 2970.5 14 3333.0 15 3673.5 16 4028.5 17 4430.0 18 4751.5 19 5136.0 20 5720.0 21 6276.5 22 7122.0 23 8583.0 24 10311.5 25 12415.5 26 15155.5 27 18540.0 28 22731.5 29 28051.0 30 35051.5 31 42191.0 32 50209.5 33 58319.0 34 65715.0 35 73174.5 36 80722.5 37 90803.5 38 97055.0 39 98178.5 40 98439.0 41 98730.5 42 101302.5 43 105914.5 44 111491.0 45 120286.5 46 128448.0 47 134154.0 48 138460.5 49 139437.0 50 138642.5 51 138212.0 52 136090.5 53 130726.5 54 123791.0 55 112165.0 56 100256.0 57 87777.5 58 74911.5 59 65713.0 60 53797.0 61 41222.0 62 32177.5 63 25054.0 64 18555.0 65 12876.5 66 9291.0 67 6924.5 68 5168.0 69 3881.5 70 3024.0 71 2326.5 72 1755.5 73 1344.0 74 1036.5 75 753.5 76 523.5 77 370.0 78 239.0 79 157.0 80 121.5 81 88.5 82 72.5 83 53.0 84 45.0 85 43.5 86 29.0 87 20.5 88 17.0 89 15.5 90 13.5 91 11.0 92 9.5 93 6.5 94 4.0 95 1.5 96 2.5 97 4.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05791135221973829 2 0.13047034885609574 3 0.018427681685424118 4 0.017777987779848268 5 0.020701610354939594 6 0.053068179469081954 7 0.05820666763136368 8 0.05008549381166554 9 0.04225963540359281 10-11 0.04121126569232269 12-13 0.042761671603355964 14-15 0.019978087596457396 16-17 0.018634402473561885 18-19 0.01835385283251777 20-21 0.021439898884003057 22-23 0.02561861195850228 24-25 0.02533806231745816 26-27 0.020745907666683404 28-29 0.019328393690881546 30-31 0.01774845623868573 32-33 0.02365476447119346 34-35 0.023477575224218224 36-37 0.023669530241774726 38-39 0.021543259278071943 40-41 0.024585008017813423 42-43 0.03168734366740397 44-45 0.04158040995685442 46-47 0.048446493277144655 48-49 0.04124079723348522 50-51 0.02978255926242023 52-53 0.041875725368479805 54-55 0.03845006659362532 56-57 0.040428679851515416 58-59 0.04559669955495967 60-61 0.02470313418246358 62-63 0.027228080951860634 64-65 0.026327368946403206 66-67 0.033444470366575024 68-69 0.04103407644534745 70-71 0.04318987895021277 72-73 0.04677796120146122 74-75 0.02823215335138695 76-77 0.0216613854427221 78-79 0.02095262845482117 80-81 0.025810566976058782 82-83 0.021749980066209717 84-85 0.013392553917211275 86-87 0.01532686986335756 88-89 0.019151204443906316 90-91 0.021676151213303366 92-93 0.02073114189610213 94-95 0.023979611423981383 96-97 0.02516087307048293 98-99 0.028320747974874567 100 0.03080139743252781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3386210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.05123602840162 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5922330387201 63.48751647958536 2 10.53853246082165 15.818597742201955 3 2.531938393085489 5.700753178464928 4 0.9032556913105309 2.711618243301749 5 0.4408278159339996 1.6542336230773693 6 0.2550822823711776 1.1486544348541583 7 0.16646606095489652 0.8745438542010961 8 0.10931337774543232 0.6563283291387412 9 0.07924118166088312 0.535243376520035 >10 0.3624371260587035 4.584403641328249 >50 0.013827499633327248 0.7168463172378179 >100 0.006115140328721261 0.7939731983908318 >500 3.864342583432239E-4 0.1834238696353655 >1k 2.1468569795167104E-4 0.3286290608654617 >5k 8.587427918066841E-5 0.4518246776225376 >10k+ 4.2937139590334204E-5 0.3534099735743791 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10967 0.3238724119295614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8368 0.2471199364481234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5653 0.16694180219183097 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.953154116253865E-5 2.953154116253865E-5 10-11 0.0 0.0 0.0 5.90630823250773E-5 2.953154116253865E-5 12-13 0.0 0.0 0.0 8.859462348761595E-5 2.953154116253865E-5 14-15 0.0 0.0 0.0 1.4765770581269324E-4 4.429731174380797E-5 16-17 0.0 0.0 0.0 1.4765770581269324E-4 5.90630823250773E-5 18-19 0.0 0.0 0.0 1.771892469752319E-4 8.859462348761595E-5 20-21 0.0 0.0 0.0 2.0672078813777054E-4 1.0336039406888527E-4 22-23 0.0 0.0 0.0 5.020361997631571E-4 1.6242347639396255E-4 24-25 0.0 0.0 0.0 9.450093172012368E-4 2.0672078813777054E-4 26-27 0.0 0.0 0.0 0.002126270963702783 2.0672078813777054E-4 28-29 0.0 0.0 0.0 0.005108956621119186 2.0672078813777054E-4 30-31 0.0 0.0 0.0 0.01771892469752319 2.362523293003092E-4 32-33 0.0 0.0 0.0 0.04773773628924373 2.6578387046284787E-4 34-35 0.0 0.0 0.0 0.0907356602219 2.6578387046284787E-4 36-37 0.0 0.0 0.0 0.15662052855552372 2.6578387046284787E-4 38-39 0.0 0.0 0.0 0.2570277685081551 2.6578387046284787E-4 40-41 0.0 0.0 0.0 0.37005974230777183 2.6578387046284787E-4 42-43 0.0 0.0 0.0 0.47922308421509596 2.6578387046284787E-4 44-45 0.0 0.0 0.0 0.6025615658804386 2.6578387046284787E-4 46-47 0.0 0.0 0.0 0.750839434057545 2.8054964104411715E-4 48-49 0.0 0.0 0.0 0.906529719066449 2.953154116253865E-4 50-51 0.0 0.0 0.0 1.0678605284373974 2.953154116253865E-4 52-53 0.0 0.0 0.0 1.2242595704342023 2.953154116253865E-4 54-55 0.0 0.0 0.0 1.4051402600547513 3.100811822066558E-4 56-57 0.0 0.0 0.0 1.6123926159334476 3.2484695278792516E-4 58-59 2.953154116253865E-5 0.0 0.0 1.840597600266965 3.2484695278792516E-4 60-61 2.953154116253865E-5 0.0 0.0 2.0667944398014297 3.2484695278792516E-4 62-63 2.953154116253865E-5 0.0 0.0 2.292607369300782 3.2484695278792516E-4 64-65 2.953154116253865E-5 0.0 0.0 2.5262313914376247 3.2484695278792516E-4 66-67 2.953154116253865E-5 0.0 0.0 2.791527991471291 3.2484695278792516E-4 68-69 2.953154116253865E-5 0.0 0.0 3.0873602050670215 3.2484695278792516E-4 70-71 2.953154116253865E-5 1.4765770581269324E-5 0.0 3.3940895573517293 3.2484695278792516E-4 72-73 2.953154116253865E-5 2.953154116253865E-5 0.0 3.6933769612634775 4.134415762755411E-4 74-75 2.953154116253865E-5 2.953154116253865E-5 0.0 3.9940523476098644 4.134415762755411E-4 76-77 2.953154116253865E-5 2.953154116253865E-5 0.0 4.326090230670868 4.134415762755411E-4 78-79 2.953154116253865E-5 2.953154116253865E-5 0.0 4.691306800227984 4.134415762755411E-4 80-81 2.953154116253865E-5 2.953154116253865E-5 0.0 5.067966841985583 4.282073468568104E-4 82-83 2.953154116253865E-5 2.953154116253865E-5 0.0 5.452703760251136 4.4297311743807976E-4 84-85 2.953154116253865E-5 2.953154116253865E-5 0.0 5.837278255040296 4.4297311743807976E-4 86-87 2.953154116253865E-5 2.953154116253865E-5 0.0 6.244016171471941 5.020361997631571E-4 88 2.953154116253865E-5 2.953154116253865E-5 0.0 6.572362611887627 5.020361997631571E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3020 0.0 48.77629 2 GGTATCA 5580 0.0 47.398773 1 GTATCAA 9395 0.0 40.32404 1 GTACCTG 3850 0.0 38.871502 1 ACCTGGG 3880 0.0 37.194374 3 CAACGCA 11240 0.0 32.78841 5 ATCAACG 11225 0.0 32.74847 3 TATCAAC 11770 0.0 31.98784 2 AACGCAG 12295 0.0 30.587112 6 TCAACGC 12140 0.0 30.35764 4 TATCACG 285 0.0 29.723438 2 CCTGGGG 3695 0.0 29.515093 4 GTACTAG 1495 0.0 28.960869 1 GTACATG 10020 0.0 28.039585 1 ATAACGC 430 0.0 27.33016 3 ACGCAGA 13570 0.0 27.089684 7 TACATGG 10295 0.0 26.83386 2 CGCAGAG 13645 0.0 26.69805 8 TAACGCC 680 0.0 26.269108 4 GTGGTAT 2240 0.0 26.261948 1 >>END_MODULE