FastQCFastQC Report
Mon 6 Jun 2016
ERR1211216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386210
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107440.31728687825031526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.26542949196889737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.1721984165187629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG33150.049.474172
GGTATCA53950.045.662121
GTACCTG40700.041.583821
ACCTGGG39250.040.2352643
GTATCAA91200.039.589741
TAACGCA3750.032.5873684
ATCAACG112800.031.125673
CAACGCA113450.031.1038655
CCTGGGG38100.030.5938344
TATCAAC117550.030.5826742
ATAACGC4150.029.4464193
CTAACGC5800.029.1730423
AACGCAG123700.028.9443746
TCAACGC121850.028.8910624
GTACATG104000.027.7556421
GTACTAG15450.027.3861081
TACATGG105000.026.9876252
TGGGGGG59850.026.9282976
GTGGTAT23750.026.7230761
CTGGGGG27800.026.7049145
Produced by FastQC (version 0.11.5)