##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211216_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3386210 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15783073111236 33.0 31.0 34.0 30.0 34.0 2 32.29323491455049 34.0 31.0 34.0 30.0 34.0 3 32.37290746882208 34.0 31.0 34.0 30.0 34.0 4 35.98843338127287 37.0 35.0 37.0 35.0 37.0 5 35.87712988857749 37.0 35.0 37.0 35.0 37.0 6 35.90277360234599 37.0 35.0 37.0 35.0 37.0 7 35.844723747198195 37.0 35.0 37.0 35.0 37.0 8 35.82553621895866 37.0 35.0 37.0 35.0 37.0 9 37.440584311073444 39.0 37.0 39.0 34.0 39.0 10-11 37.413057813898135 39.0 37.0 39.0 34.0 39.0 12-13 37.31950292509915 39.0 37.0 39.0 34.0 39.0 14-15 38.55585256082759 40.0 38.0 41.0 34.0 41.0 16-17 38.50440758251851 40.0 38.0 41.0 34.0 41.0 18-19 38.525305872937594 40.0 38.0 41.0 34.0 41.0 20-21 38.4815094161319 40.0 38.0 41.0 34.0 41.0 22-23 38.352760460810174 40.0 38.0 41.0 34.0 41.0 24-25 38.29677692759752 40.0 38.0 41.0 34.0 41.0 26-27 38.16368004347043 40.0 38.0 41.0 33.5 41.0 28-29 38.1307091113664 40.0 37.5 41.0 33.5 41.0 30-31 38.13521651049403 40.0 37.5 41.0 33.5 41.0 32-33 37.992074324982795 40.0 37.0 41.0 33.0 41.0 34-35 37.83848860525484 40.0 37.0 41.0 33.0 41.0 36-37 37.680680761086876 40.0 37.0 41.0 33.0 41.0 38-39 37.5951481154447 40.0 37.0 41.0 33.0 41.0 40-41 37.49711078167036 40.0 36.5 41.0 32.0 41.0 42-43 37.35360019018312 40.0 36.0 41.0 32.0 41.0 44-45 37.22412283939862 40.0 36.0 41.0 31.0 41.0 46-47 36.99747387196896 39.5 36.0 41.0 31.0 41.0 48-49 36.78027012500701 39.0 35.5 41.0 30.5 41.0 50-51 36.5904994078926 39.0 35.0 41.0 30.0 41.0 52-53 36.401684479107914 39.0 35.0 40.5 30.0 41.0 54-55 36.1753492843031 39.0 35.0 40.0 29.0 41.0 56-57 35.939581567593265 38.0 35.0 40.0 28.5 41.0 58-59 35.67976587394166 38.0 34.5 40.0 28.0 41.0 60-61 35.42011009358545 38.0 34.0 40.0 28.0 41.0 62-63 35.11892735536189 37.0 34.0 40.0 27.5 41.0 64-65 34.74742691681851 36.5 34.0 39.5 27.0 41.0 66-67 34.43860422123849 36.0 33.5 39.0 26.0 41.0 68-69 34.10815306788415 35.5 33.0 39.0 26.0 40.0 70-71 33.77107813750476 35.0 33.0 38.5 26.0 40.0 72-73 33.41812970843509 35.0 32.5 37.5 26.0 40.0 74-75 33.06620233240112 35.0 32.0 37.0 25.0 39.0 76-77 32.05945865731895 34.0 30.5 36.0 24.5 38.5 78-79 32.54888813747523 35.0 32.0 36.0 25.0 38.5 80-81 32.467902906198965 35.0 32.0 36.0 25.0 37.5 82-83 32.205491390079175 35.0 32.0 35.0 25.0 37.0 84-85 31.94250578079918 34.0 31.5 35.0 25.0 36.5 86-87 31.694553350205688 34.0 31.0 35.0 24.0 36.0 88-89 31.4716734933746 34.0 31.0 35.0 24.0 36.0 90-91 31.25483741410013 34.0 31.0 35.0 23.0 35.5 92-93 31.07573511388839 34.0 31.0 35.0 23.0 35.0 94-95 30.88225656412331 34.0 30.5 35.0 20.5 35.0 96-97 30.709259319416102 34.0 30.0 35.0 20.0 35.0 98-99 30.532660260291003 34.0 30.0 35.0 20.0 35.0 100 28.777975967231804 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3604468880794869 1101 2 -0.28992648037622715 1101 3 -0.36244249929300665 1101 4 -0.2390347584702468 1101 5 -0.19847379103238438 1101 6 -0.2547946794560545 1101 7 -0.23338188263761594 1101 8 -0.35339113036562253 1101 9 -0.5189982568483771 1101 10-11 -0.5031640192559976 1101 12-13 -0.3925161336058878 1101 14-15 -0.6653128270107658 1101 16-17 -0.6405539164778347 1101 18-19 -0.5886029528426562 1101 20-21 -0.652250238247575 1101 22-23 -0.6741224687824925 1101 24-25 -0.6403915357877636 1101 26-27 -0.7486558751898897 1101 28-29 -0.8247012578090462 1101 30-31 -0.6999154108260228 1101 32-33 -0.5713658750599393 1101 34-35 -0.6499166911422591 1101 36-37 -0.6269942501995303 1101 38-39 -0.6227483355311492 1101 40-41 -0.6823122281258875 1101 42-43 -0.5699434408451793 1101 44-45 -0.6262509658109892 1101 46-47 -0.6073024121818165 1101 48-49 -0.678480655694905 1101 50-51 -0.8360514734958429 1101 52-53 -0.8100324282384008 1101 54-55 -0.7064395529873622 1101 56-57 -0.6195820540307153 1101 58-59 -0.5978760868414028 1101 60-61 -0.6335840464537768 1101 62-63 -0.9500640818949293 1101 64-65 -0.9425883397522128 1101 66-67 -0.8890938749019384 1101 68-69 -0.797074798375121 1101 70-71 -0.7761854263472898 1101 72-73 -1.0168839996889645 1101 74-75 -1.0604448015767431 1101 76-77 -1.0473879155857198 1101 78-79 -0.9110540442921256 1101 80-81 -0.8984323813260957 1101 82-83 -0.8489158383052029 1101 84-85 -0.7237510426624105 1101 86-87 -0.8698376669837806 1101 88-89 -0.8671349514639033 1101 90-91 -1.0219934033772589 1101 92-93 -1.0354641029772154 1101 94-95 -1.1301433999194685 1101 96-97 -1.108479869330548 1101 98-99 -1.2584757675371527 1101 100 -0.9653067942011688 1102 1 -0.43262920008386274 1102 2 -0.4183392050432744 1102 3 -0.3800292374476051 1102 4 -0.224895781888236 1102 5 -0.2790965017106828 1102 6 -0.26331311856922923 1102 7 -0.32562438116298154 1102 8 -0.29803607129660037 1102 9 -0.4051246827935273 1102 10-11 -0.5017514873054836 1102 12-13 -0.5117167805611302 1102 14-15 -0.5927592809724374 1102 16-17 -0.614096690117421 1102 18-19 -0.6400699179995541 1102 20-21 -0.6689249329631366 1102 22-23 -0.6112612201311265 1102 24-25 -0.6649572028069883 1102 26-27 -0.751198205082872 1102 28-29 -0.7684319333162506 1102 30-31 -0.6492684664235497 1102 32-33 -0.6758519429989107 1102 34-35 -0.6054000942170106 1102 36-37 -0.6512389759123067 1102 38-39 -0.7117767602436942 1102 40-41 -0.5699581122622774 1102 42-43 -0.587102148868361 1102 44-45 -0.5579369666351894 1102 46-47 -0.5534734772582155 1102 48-49 -0.5199842088110671 1102 50-51 -0.5564193561264972 1102 52-53 -0.6602613902160215 1102 54-55 -0.6857439330286894 1102 56-57 -0.6084763575772456 1102 58-59 -0.7298427947900166 1102 60-61 -0.8434794440839042 1102 62-63 -0.7711296568914747 1102 64-65 -0.615059498932375 1102 66-67 -0.75574595956801 1102 68-69 -0.8451005053293699 1102 70-71 -0.9959186342667508 1102 72-73 -0.8032413061058605 1102 74-75 -1.055666071151613 1102 76-77 -0.7963793512978512 1102 78-79 -0.5338446598425932 1102 80-81 -0.4789248620213833 1102 82-83 -0.5635162615011637 1102 84-85 -0.6618334329890381 1102 86-87 -0.6501813614138392 1102 88-89 -0.6451848531221103 1102 90-91 -0.6734262150030297 1102 92-93 -0.8135954593438974 1102 94-95 -0.8831996044270412 1102 96-97 -0.9046616541284003 1102 98-99 -1.0757276038178496 1102 100 -0.9432502898882404 1103 1 -0.31493967791299227 1103 2 -0.31393995601042946 1103 3 -0.3631379592682329 1103 4 -0.21893765943854504 1103 5 -0.21011432538733033 1103 6 -0.3085063577130427 1103 7 -0.38800445628451996 1103 8 -0.44643722464444124 1103 9 -0.44678105614519836 1103 10-11 -0.5261246089048157 1103 12-13 -0.5144693371309117 1103 14-15 -0.7134388818359483 1103 16-17 -0.6851470468764802 1103 18-19 -0.6214409316934848 1103 20-21 -0.7073612056534273 1103 22-23 -0.7217401458359447 1103 24-25 -0.8245536034778453 1103 26-27 -0.7868105404260319 1103 28-29 -0.7546297358641922 1103 30-31 -0.6828145868886395 1103 32-33 -0.6613469035160335 1103 34-35 -0.7580989910633136 1103 36-37 -0.7792782452710867 1103 38-39 -0.7182926355354766 1103 40-41 -0.7535453335720703 1103 42-43 -0.6802164073545072 1103 44-45 -0.756447115326516 1103 46-47 -0.8106928152832253 1103 48-49 -0.8516813188620205 1103 50-51 -0.8460954301589112 1103 52-53 -0.9030407590609784 1103 54-55 -0.9314635174725652 1103 56-57 -0.9244666448258982 1103 58-59 -0.9436027741330335 1103 60-61 -0.9199018767656213 1103 62-63 -0.8064660900126484 1103 64-65 -0.8439049779636107 1103 66-67 -0.8937706237990355 1103 68-69 -0.9124644070150723 1103 70-71 -1.0361689614803637 1103 72-73 -0.8937396142779903 1103 74-75 -0.907967294307781 1103 76-77 -0.861619778308004 1103 78-79 -0.7042960594644612 1103 80-81 -0.6329982526331364 1103 82-83 -0.757820685826573 1103 84-85 -0.8729935485555522 1103 86-87 -0.7940988708909309 1103 88-89 -0.8206992705908291 1103 90-91 -0.8090779339142173 1103 92-93 -0.9153714130841841 1103 94-95 -0.8602467533960336 1103 96-97 -1.0548636203481188 1103 98-99 -1.078356226806104 1103 100 -1.0285546637298566 1104 1 -0.3702758475068251 1104 2 -0.29442187787993745 1104 3 -0.34567010961428224 1104 4 -0.2287263943936395 1104 5 -0.25863134901071305 1104 6 -0.28189702229493463 1104 7 -0.24195825190407305 1104 8 -0.23690363192659447 1104 9 -0.4506330372852787 1104 10-11 -0.47339743534748635 1104 12-13 -0.46630169474214966 1104 14-15 -0.7047801057914498 1104 16-17 -0.6506240598335822 1104 18-19 -0.6243253722592854 1104 20-21 -0.7267956504614901 1104 22-23 -0.6702814110020583 1104 24-25 -0.7938356295859847 1104 26-27 -0.7414694619197988 1104 28-29 -0.8576238694108511 1104 30-31 -0.8310004104344557 1104 32-33 -0.6780435690918338 1104 34-35 -0.641745646336453 1104 36-37 -0.5678050261154226 1104 38-39 -0.5859880526718229 1104 40-41 -0.6872938033246854 1104 42-43 -0.6226700151021021 1104 44-45 -0.737730220183316 1104 46-47 -0.6348686028303518 1104 48-49 -0.7464527211140606 1104 50-51 -0.7421001208974332 1104 52-53 -0.6103204943433269 1104 54-55 -0.6956142150229567 1104 56-57 -0.67635203081187 1104 58-59 -0.6731180644861183 1104 60-61 -0.6913301884633825 1104 62-63 -0.6826242297166587 1104 64-65 -0.7976869616490418 1104 66-67 -0.859379147125459 1104 68-69 -0.79189606404573 1104 70-71 -0.8395927763719513 1104 72-73 -0.7838460346505798 1104 74-75 -0.7958213723413081 1104 76-77 -0.7437007329399563 1104 78-79 -0.5511779910305847 1104 80-81 -0.6131962454333966 1104 82-83 -0.5866229718040543 1104 84-85 -0.5761882808683154 1104 86-87 -0.6313144872927587 1104 88-89 -0.621984981413469 1104 90-91 -0.6552562547025502 1104 92-93 -0.6593261783654505 1104 94-95 -0.6968411802653627 1104 96-97 -0.9581359255976238 1104 98-99 -0.9530865396432127 1104 100 -0.9932286755839712 1105 1 -0.37053090316713977 1105 2 -0.4424222103353799 1105 3 -0.4942330190149775 1105 4 -0.2667172207012527 1105 5 -0.3503854150966248 1105 6 -0.351158399803289 1105 7 -0.38328713864585495 1105 8 -0.3980245262317368 1105 9 -0.5024436207965266 1105 10-11 -0.539928782259544 1105 12-13 -0.5631005124474839 1105 14-15 -0.7070852167744235 1105 16-17 -0.678155785952633 1105 18-19 -0.7665051371509506 1105 20-21 -0.8085472231315762 1105 22-23 -0.7307734970041295 1105 24-25 -0.7443101577849589 1105 26-27 -0.8599646335239512 1105 28-29 -0.8110190706243188 1105 30-31 -0.7386242890466619 1105 32-33 -0.7762880485133579 1105 34-35 -0.7424310759571924 1105 36-37 -0.652325329273225 1105 38-39 -0.7487857691184985 1105 40-41 -0.8891080230673438 1105 42-43 -0.9150300331793702 1105 44-45 -0.8817369316275645 1105 46-47 -0.8152451605659152 1105 48-49 -0.8494118342891639 1105 50-51 -0.9138188116463368 1105 52-53 -0.8960049110138897 1105 54-55 -0.9560265636648282 1105 56-57 -0.8803253928194863 1105 58-59 -0.9560158292427232 1105 60-61 -0.9128660287185397 1105 62-63 -0.9770787170859521 1105 64-65 -1.0061619781419537 1105 66-67 -0.9811167982238587 1105 68-69 -0.9734328393318208 1105 70-71 -0.9977036502622454 1105 72-73 -0.9192166964966475 1105 74-75 -0.9900833991766973 1105 76-77 -0.915235794002669 1105 78-79 -0.808496166473553 1105 80-81 -0.7561255934571065 1105 82-83 -0.7261415347654037 1105 84-85 -0.8825218167916837 1105 86-87 -0.9692549258222662 1105 88-89 -0.7894035687895098 1105 90-91 -0.8992063448291461 1105 92-93 -1.0840177480226565 1105 94-95 -0.8818536733175613 1105 96-97 -0.8808356196347233 1105 98-99 -1.0795110332979867 1105 100 -0.9982710896260585 1106 1 -0.4471372160066629 1106 2 -0.41487242234932964 1106 3 -0.43302555905189166 1106 4 -0.2623833523224377 1106 5 -0.33620426995987174 1106 6 -0.38293957840216564 1106 7 -0.46984372885504655 1106 8 -0.4831208082756504 1106 9 -0.6337887460442246 1106 10-11 -0.6004996775283118 1106 12-13 -0.726066302650402 1106 14-15 -0.8029089863876706 1106 16-17 -0.7402628020196786 1106 18-19 -0.7633442732006444 1106 20-21 -0.795289162157303 1106 22-23 -0.698833111875949 1106 24-25 -0.8211703122335976 1106 26-27 -0.7807561174124515 1106 28-29 -0.8542490024975109 1106 30-31 -0.8501864998476094 1106 32-33 -0.862302447685174 1106 34-35 -0.7581925223576604 1106 36-37 -0.9202074380146854 1106 38-39 -0.7682382450545191 1106 40-41 -0.8747462347257553 1106 42-43 -0.7270800590602349 1106 44-45 -0.8826602772129206 1106 46-47 -0.8625564263231738 1106 48-49 -0.7687738579113343 1106 50-51 -0.8888027341396878 1106 52-53 -0.8585532088895746 1106 54-55 -0.8461850382100806 1106 56-57 -1.0146251193711606 1106 58-59 -0.8769678267748944 1106 60-61 -0.887301747448447 1106 62-63 -0.868461173519826 1106 64-65 -0.917354798424185 1106 66-67 -0.835838356722455 1106 68-69 -0.8678603493835269 1106 70-71 -0.9080578259697845 1106 72-73 -0.9882291977384057 1106 74-75 -0.9991435316658119 1106 76-77 -0.9144611980317201 1106 78-79 -0.8433809574136859 1106 80-81 -0.6391636932265286 1106 82-83 -0.6234697930093702 1106 84-85 -0.621047182652994 1106 86-87 -0.6737419880043092 1106 88-89 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1.1293016192726952 2310 68-69 1.158307493036375 2310 70-71 1.17952371043949 2310 72-73 1.2058220081997462 2310 74-75 1.092475243624719 2310 76-77 1.164165985559137 2310 78-79 1.024823268694142 2310 80-81 0.926620979662026 2310 82-83 0.9101648941599194 2310 84-85 1.091191409678995 2310 86-87 1.0384280072574619 2310 88-89 1.0665946625502727 2310 90-91 1.1569963725944348 2310 92-93 1.1746567005320294 2310 94-95 1.268128794654963 2310 96-97 1.2591689687938121 2310 98-99 1.2388919006929093 2310 100 1.4525260684770345 2311 1 0.3973319723201314 2311 2 0.3210122083759259 2311 3 0.387115868854778 2311 4 0.21873909665715274 2311 5 0.3725365064809978 2311 6 0.45032921771563394 2311 7 0.4933093988455468 2311 8 0.4403411009179976 2311 9 0.5571356041518811 2311 10-11 0.5873069434914271 2311 12-13 0.6418768436635034 2311 14-15 0.8000949024642878 2311 16-17 0.6540759269572547 2311 18-19 0.723239187545218 2311 20-21 0.6893818642337095 2311 22-23 0.8593434486505416 2311 24-25 0.8945126466176987 2311 26-27 0.12487399293787149 2311 28-29 0.4920636512284702 2311 30-31 0.7097275797907656 2311 32-33 0.8669187847817525 2311 34-35 0.8790232923069681 2311 36-37 0.9476488738911826 2311 38-39 0.9654368862357785 2311 40-41 0.8320625800053492 2311 42-43 0.8366696916543859 2311 44-45 0.8538039420252588 2311 46-47 0.5055184257674981 2311 48-49 0.7147907568596565 2311 50-51 0.9147612158702856 2311 52-53 0.8265644538106898 2311 54-55 0.9013183686901343 2311 56-57 0.9676997161004905 2311 58-59 0.9873357529631974 2311 60-61 1.0423449320702645 2311 62-63 0.9952599093758678 2311 64-65 1.058953534355176 2311 66-67 1.0646095260136903 2311 68-69 1.0998717353070262 2311 70-71 1.0658382552640049 2311 72-73 1.177617458513673 2311 74-75 1.062982124785691 2311 76-77 1.062290862727437 2311 78-79 0.8325895936731982 2311 80-81 0.8578835214248031 2311 82-83 0.8504865321917165 2311 84-85 1.032739877773782 2311 86-87 0.9582201372366619 2311 88-89 1.028245766225396 2311 90-91 1.0673380257280627 2311 92-93 1.1162215892852991 2311 94-95 1.1475919813487963 2311 96-97 1.2149107688082914 2311 98-99 1.2950848162777966 2311 100 1.3198640857924246 2312 1 0.18448579936819698 2312 2 0.33221134320226753 2312 3 0.4037564906207223 2312 4 0.10556796613597896 2312 5 0.3052521789568843 2312 6 0.39055927525588885 2312 7 0.19784379881242842 2312 8 0.3724937895796714 2312 9 0.5067702856106706 2312 10-11 0.5398139807891056 2312 12-13 0.6547258625814152 2312 14-15 0.8107414024435897 2312 16-17 0.8259106953473676 2312 18-19 0.7924846443486331 2312 20-21 0.8302393279088278 2312 22-23 0.8566744500166052 2312 24-25 0.8546243637183366 2312 26-27 0.9401477748644353 2312 28-29 0.9138371019880864 2312 30-31 0.9486992029227679 2312 32-33 0.8060999744990269 2312 34-35 0.9033619933046069 2312 36-37 0.9567461334846712 2312 38-39 0.6852836180319457 2312 40-41 0.8983937676528129 2312 42-43 0.9374360326735527 2312 44-45 0.6763509780810608 2312 46-47 0.7594567252252133 2312 48-49 0.9848620612434758 2312 50-51 0.8216039529651198 2312 52-53 0.9809265434741405 2312 54-55 0.8532393957216442 2312 56-57 0.8571116877857179 2312 58-59 0.9781652744269493 2312 60-61 1.073265576189982 2312 62-63 1.1671296055301923 2312 64-65 1.1384152328276755 2312 66-67 1.1249797085694766 2312 68-69 1.1060456703983021 2312 70-71 1.017434324726274 2312 72-73 1.0021033735693479 2312 74-75 1.0062953983709946 2312 76-77 1.0574829913030044 2312 78-79 0.9630828254486659 2312 80-81 0.8500889380742223 2312 82-83 0.8833093164778418 2312 84-85 0.9296582140738145 2312 86-87 0.9676405433301376 2312 88-89 0.9882219118560585 2312 90-91 1.0272187021365653 2312 92-93 1.110975439101093 2312 94-95 1.0204399136251574 2312 96-97 0.7365349357164384 2312 98-99 0.6072562365700485 2312 100 0.7431518491736391 2313 1 0.08453927092781299 2313 2 0.23087209662300268 2313 3 0.2781802873776158 2313 4 0.17376453676074988 2313 5 0.31818498695737674 2313 6 0.24354878008467296 2313 7 0.22593435614797386 2313 8 0.34358082673588086 2313 9 0.5625566659065839 2313 10-11 0.39685169819070865 2313 12-13 0.40755937385768704 2313 14-15 0.5682079120948131 2313 16-17 0.8067370051282126 2313 18-19 0.7355543134456894 2313 20-21 0.6999986146810784 2313 22-23 0.6031116429129995 2313 24-25 0.5004955637665702 2313 26-27 0.7765102975971274 2313 28-29 0.6129613389320809 2313 30-31 0.8487570449368036 2313 32-33 0.9133198258376964 2313 34-35 0.9662997354847889 2313 36-37 0.9928962521330078 2313 38-39 0.9911651665561507 2313 40-41 0.9947233650288254 2313 42-43 1.0259242430877507 2313 44-45 0.9454687074817798 2313 46-47 0.9124706095888158 2313 48-49 1.0691435509375609 2313 50-51 1.064079265598295 2313 52-53 1.0461366369182556 2313 54-55 0.9407393450497068 2313 56-57 0.7072730335613784 2313 58-59 0.8135236694238088 2313 60-61 0.7240100862658494 2313 62-63 1.0210571112193847 2313 64-65 1.11662326037699 2313 66-67 1.163476001157342 2313 68-69 1.1880821400035018 2313 70-71 1.1487955844609914 2313 72-73 1.177228305225583 2313 74-75 1.2274888149211591 2313 76-77 1.1680618852258675 2313 78-79 1.0934275111534575 2313 80-81 0.9279343952592285 2313 82-83 0.903088789682279 2313 84-85 0.9555374798273455 2313 86-87 1.0078369743537259 2313 88-89 0.9921281796704164 2313 90-91 0.9012579939151948 2313 92-93 1.0608117117444742 2313 94-95 1.179375605365479 2313 96-97 1.25458636925082 2313 98-99 1.2399224253159176 2313 100 1.2278195350218297 2314 1 -0.3853419846346604 2314 2 0.03678935541221051 2314 3 0.31380581104325245 2314 4 0.2579969499691259 2314 5 0.34530153399370533 2314 6 0.37898858248543377 2314 7 0.45759946640840354 2314 8 0.4256191882621465 2314 9 0.4504332986563 2314 10-11 0.5882838188832196 2314 12-13 0.46452563867638474 2314 14-15 0.795301944325395 2314 16-17 0.8404928316030649 2314 18-19 0.8608950348670845 2314 20-21 0.8732821393608248 2314 22-23 0.8789088174601787 2314 24-25 0.82189432454485 2314 26-27 0.7266845209033193 2314 28-29 0.7312697005423914 2314 30-31 0.7719507113642123 2314 32-33 0.8676274983012959 2314 34-35 0.928242314386793 2314 36-37 0.9126668093930306 2314 38-39 0.593268257692209 2314 40-41 0.6206622178126864 2314 42-43 0.775834384835413 2314 44-45 0.849404084812754 2314 46-47 0.8394809527578175 2314 48-49 0.8659095490864459 2314 50-51 0.7666218065229344 2314 52-53 0.8101734946966701 2314 54-55 0.7317316688224764 2314 56-57 0.8449346885877844 2314 58-59 0.9172109880401607 2314 60-61 0.9404138183278405 2314 62-63 0.8376786772243037 2314 64-65 0.9303094060553931 2314 66-67 0.6658832949069122 2314 68-69 0.6869298776766044 2314 70-71 0.8158918005729205 2314 72-73 0.8705882219043559 2314 74-75 0.8912753789474266 2314 76-77 0.8394714192503869 2314 78-79 0.7290738870492532 2314 80-81 0.808192168774525 2314 82-83 0.7948882177806311 2314 84-85 0.8040826896425592 2314 86-87 0.9362800557433211 2314 88-89 0.92958004567096 2314 90-91 0.9212002532129162 2314 92-93 0.8160252564859682 2314 94-95 0.8850292941578815 2314 96-97 0.8978703007439357 2314 98-99 0.9889754271296489 2314 100 1.0098663494965407 2315 1 0.4317752100734751 2315 2 0.48999415074636943 2315 3 0.4364882009361679 2315 4 0.2674762190005424 2315 5 0.3242246799026205 2315 6 0.41909370429370796 2315 7 0.34835405898602545 2315 8 0.2655753372585039 2315 9 0.4484566040965703 2315 10-11 0.5483513095009513 2315 12-13 0.38999355708944705 2315 14-15 0.5686091263356516 2315 16-17 0.7845942133553265 2315 18-19 0.6598098468510756 2315 20-21 0.4663621888838918 2315 22-23 0.8281967401519168 2315 24-25 0.9221477897362931 2315 26-27 0.8100656619927804 2315 28-29 0.7755227758243564 2315 30-31 0.8129248712720241 2315 32-33 0.6872476332464927 2315 34-35 0.8196938731779042 2315 36-37 0.8525542617669544 2315 38-39 0.8859427160621678 2315 40-41 0.8733013741187037 2315 42-43 0.8970470465874953 2315 44-45 0.8674099812628313 2315 46-47 0.8511140032446107 2315 48-49 0.7681332283641424 2315 50-51 0.8737638228258575 2315 52-53 0.9342240512786475 2315 54-55 0.7260734166812455 2315 56-57 0.8022995094546417 2315 58-59 0.8435410676833115 2315 60-61 0.8739762024646041 2315 62-63 0.9470359764176166 2315 64-65 1.0473607209548774 2315 66-67 1.122545594010738 2315 68-69 1.128390151173008 2315 70-71 0.9182155938565089 2315 72-73 0.9993907853610438 2315 74-75 0.49663193171512177 2315 76-77 0.5335455637948598 2315 78-79 0.7716188860997022 2315 80-81 0.8507017924878468 2315 82-83 0.7398986976491813 2315 84-85 0.8381515417002525 2315 86-87 0.873299973679746 2315 88-89 0.9014727787598957 2315 90-91 0.9554410570366656 2315 92-93 0.9592656121420404 2315 94-95 0.9848076547578515 2315 96-97 1.1125016603016213 2315 98-99 1.082058900072436 2315 100 1.0164860420398156 2316 1 0.2793369056040689 2316 2 0.051625531795316704 2316 3 0.18763907863607443 2316 4 0.06944952507124214 2316 5 0.3057381544617499 2316 6 0.35494459159460234 2316 7 0.42024559859279265 2316 8 0.4305117737631079 2316 9 0.6615175733036267 2316 10-11 0.6280924369974556 2316 12-13 0.3897404513050162 2316 14-15 0.4244417625711705 2316 16-17 0.7419619107464399 2316 18-19 0.7198819406424803 2316 20-21 0.6444067907673627 2316 22-23 0.7320515271567487 2316 24-25 0.4832057768168099 2316 26-27 0.6474318080310866 2316 28-29 0.8843004856538599 2316 30-31 0.47985018864530105 2316 32-33 0.6178741010042401 2316 34-35 0.7960181889973015 2316 36-37 0.8259838524983962 2316 38-39 0.744825580285891 2316 40-41 0.7284101988837861 2316 42-43 0.7201943885264157 2316 44-45 0.5645794179704282 2316 46-47 0.796504270490864 2316 48-49 0.9127751271807725 2316 50-51 0.8493013723954732 2316 52-53 0.8674872367512734 2316 54-55 0.7931573555905445 2316 56-57 0.9022353047701799 2316 58-59 0.7779298723567862 2316 60-61 0.8657517655820897 2316 62-63 0.8472324933609698 2316 64-65 0.8939509522479767 2316 66-67 0.9158498891074558 2316 68-69 0.8707605067569091 2316 70-71 0.8248905711667405 2316 72-73 0.8436573879149307 2316 74-75 0.7964043938425363 2316 76-77 0.7801607257932801 2316 78-79 0.45199600085184244 2316 80-81 0.3645316861619676 2316 82-83 0.3365364283998886 2316 84-85 0.45459843032188374 2316 86-87 0.589091005230717 2316 88-89 0.5865722372017501 2316 90-91 0.4156055396473377 2316 92-93 0.23016741071260327 2316 94-95 0.25076632370449303 2316 96-97 0.4619974920825314 2316 98-99 0.7401763383379993 2316 100 0.42826519679638864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 26.0 13 58.0 14 318.0 15 1002.0 16 2395.0 17 4949.0 18 8249.0 19 11664.0 20 15804.0 21 20939.0 22 25723.0 23 26959.0 24 27400.0 25 29206.0 26 33787.0 27 41534.0 28 51018.0 29 62760.0 30 77582.0 31 96613.0 32 122020.0 33 160628.0 34 225288.0 35 316494.0 36 487759.0 37 753751.0 38 674656.0 39 107618.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.327208701055014 19.62907714229917 13.256682363806702 23.787031792839112 2 15.848751258781943 20.3441015176259 40.46255252922884 23.344594694363316 3 18.931607903821675 25.68691841321123 29.924428786165063 25.457044896802028 4 12.575953575744721 16.48833263862774 38.19011812555334 32.7455956600742 5 13.03640353079106 37.052634065814 34.58279315222624 15.32816925116871 6 30.584783578100588 36.67265172567561 18.450539098283922 14.292025597939881 7 27.35870787578673 32.09561464023134 22.398245100077492 18.14743238390444 8 26.05659424548389 35.63851621724583 20.174147498235488 18.130742039034793 9 26.26343682697726 16.28176017560171 20.955602207307837 36.49920079011319 10-11 25.368248820837426 26.17751987444316 27.9090353761323 20.545195928587113 12-13 25.478163710780443 23.816733725423664 28.36227350965733 22.34282905413856 14-15 22.71169347396274 25.352274891803656 26.344609968977075 25.59142166525653 16-17 22.03212865874943 27.830135745163158 27.24933686689319 22.88839872919422 18-19 21.597715439975666 27.22474388770927 29.386969502777443 21.790571169537625 20-21 22.623862666520978 26.5846034356995 29.166678971475484 21.62485492630404 22-23 22.544363757711423 26.42910510570815 28.90835181515618 22.118179321424247 24-25 22.074444051344962 26.32926524587782 29.41417414359603 22.182116559181193 26-27 22.095910152708502 26.601268682501573 29.00382140399682 22.2989997607931 28-29 21.787317007480944 26.990399869588483 29.1205580053653 22.101725117565273 30-31 22.520685136735864 26.63729092263816 29.141405505463915 21.70061843516206 32-33 22.43635439423348 26.7226701625655 28.811274775846456 22.02970066735456 34-35 22.53456805185038 26.721652779578875 28.992780211025444 21.7509989575453 36-37 22.43548944332425 26.635486755061283 28.83441201115895 22.094611790455517 38-39 22.314439689899217 26.915529275505474 28.85396194351919 21.916069091076118 40-41 22.39377501726143 26.402768089654305 29.083649866722265 22.119807026361997 42-43 22.213408690018063 26.63379704784734 28.74211470237939 22.410679559755206 44-45 22.123776581393656 27.053645753112843 28.766509330521238 22.056068334972263 46-47 22.191750152261893 26.67526481164126 28.36668621998846 22.766298816108392 48-49 22.396530735558954 26.755393703275587 28.435375676520152 22.412699884645303 50-51 22.268263814385108 26.976093731886813 28.247791714137826 22.50785073959025 52-53 22.144952533830306 27.32017901062737 27.898057567821212 22.636810887721115 54-55 22.09094124327074 27.092702239529455 28.106460161243756 22.709896355956044 56-57 22.451205918620445 27.000280107950093 27.82380043438143 22.72471353904804 58-59 22.046869568478993 27.180161770538884 28.19777684384125 22.575191817140873 60-61 22.33400132042013 27.383714946367345 27.907423516561437 22.374860216651086 62-63 22.394301746771898 27.14487396566111 27.790088508799272 22.67073577876772 64-65 22.104984837361684 26.98202197068507 27.86349341869118 23.04949977326206 66-67 22.10270416740171 26.954517637679448 27.791582339656053 23.151195855262788 68-69 21.880368752596652 26.999975769510126 27.916006484315485 23.203648993577737 70-71 21.93848003947489 26.722579577654137 27.999604069222883 23.339336313648086 72-73 22.0471417337991 26.986329963773375 27.821198283848137 23.145330018579386 74-75 22.21393899771966 27.08718793768192 27.60592918035073 23.092943884247692 76-77 22.080452612411626 26.881365305150617 27.619471881288497 23.418710201149256 78-79 21.82371228246251 26.960464269010675 27.683385122084452 23.53243832644236 80-81 21.843781622133402 27.08792645628484 27.684484255324556 23.38380766625721 82-83 22.158214359360386 26.84446340438782 28.073933340699348 22.923388895552442 84-85 22.252056390863032 27.04534770652475 27.50865798243673 23.193937920175483 86-87 21.673410971264076 27.218465974442807 27.707722931783117 23.40040012251 88-89 21.86836781570472 26.712522982460264 27.73835746205373 23.680751739781293 90-91 22.033967162082668 26.64796016501983 27.793159707767295 23.52491296513021 92-93 22.030655302740463 26.724065346219326 27.71726654815867 23.528012802881534 94-95 21.482072958111804 26.72308152607797 27.900781835403727 23.894063680406504 96-97 21.516441735837155 26.750889967685325 27.796494941935773 23.936173354541744 98-99 21.30460846991497 26.779024640743515 27.669413880867992 24.24695300847352 100 21.46152228217072 26.585612124563678 27.62512968490409 24.327735908361515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1066.0 1 768.5 2 1168.0 3 1753.5 4 1194.0 5 787.0 6 1050.5 7 1909.0 8 2978.5 9 3362.5 10 2876.5 11 2420.5 12 2470.0 13 2723.5 14 3070.5 15 3454.0 16 3800.0 17 4204.0 18 4746.5 19 5206.0 20 5742.5 21 6403.5 22 7302.5 23 8734.5 24 10437.5 25 12481.5 26 15292.0 27 18712.5 28 22773.0 29 28365.5 30 35706.5 31 42620.5 32 50698.0 33 58867.0 34 66131.5 35 74224.5 36 81628.5 37 91284.0 38 97554.0 39 98623.0 40 98936.0 41 98773.0 42 101152.0 43 106389.0 44 112435.0 45 121304.5 46 130016.5 47 135132.5 48 139529.5 49 141170.5 50 139449.0 51 138723.5 52 136637.5 53 131853.5 54 125731.5 55 113756.5 56 100834.0 57 87933.5 58 74743.5 59 64812.0 60 52137.0 61 39167.5 62 30210.0 63 23265.5 64 16910.0 65 11279.5 66 7941.0 67 5911.5 68 4322.5 69 3110.0 70 2325.0 71 1729.5 72 1324.0 73 1001.0 74 777.5 75 554.0 76 337.0 77 208.0 78 126.5 79 71.5 80 42.0 81 27.5 82 15.5 83 9.0 84 6.0 85 8.0 86 6.5 87 4.5 88 4.5 89 3.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044799347943571134 2 0.0 3 0.0 4 0.031982659079029356 5 0.0 6 0.0 7 0.0010040723995263141 8 0.0 9 0.010808544065489147 10-11 4.282073468568104E-4 12-13 1.4765770581269324E-5 14-15 1.4765770581269324E-4 16-17 3.543784939504638E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.725046586006184E-4 26-27 5.90630823250773E-4 28-29 1.771892469752319E-4 30-31 0.002451117916490708 32-33 0.015607419504401678 34-35 0.02749386482232348 36-37 0.03320821803727471 38-39 0.02346280945363696 40-41 0.0014175139758018551 42-43 8.859462348761595E-5 44-45 0.010616589047932645 46-47 0.017600798532873035 48-49 0.0038981634334551017 50-51 0.011295814494671035 52-53 0.01114815678885834 54-55 0.010114552848169487 56-57 4.577388880193491E-4 58-59 0.008948056972249211 60-61 0.004917001603562685 62-63 0.0014618112875456632 64-65 0.036766768747360616 66-67 0.06092356941831724 68-69 0.060362470136228995 70-71 0.0525661432693188 72-73 0.045611465325540945 74-75 0.035113002442258456 76-77 0.031362496714616044 78-79 0.014780536351850594 80-81 9.893066289450448E-4 82-83 0.03827287734665009 84-85 0.05200504398723057 86-87 0.058959721931008416 88-89 0.07027030219626071 90-91 0.04863844829470115 92-93 0.03977898594593956 94-95 0.01355497739360524 96-97 0.0038981634334551017 98-99 0.023049367877361414 100 0.03195312753786682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3386210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2817252138633 #Duplication Level Percentage of deduplicated Percentage of total 1 83.1541437282085 60.9367911108467 2 11.423507486505867 16.74268673209266 3 2.805234709441692 6.167173174130933 4 1.0276279956052925 3.012254095840807 5 0.4926547764309226 1.8051295975854067 6 0.2780926084566352 1.2227463670155374 7 0.1793691078333835 0.9201134370471277 8 0.12981174401212944 0.7610262843383387 9 0.0930524427901854 0.6137139188726192 >10 0.3925454511635739 4.827783190065911 >50 0.016554722073414872 0.8326749212785836 >100 0.006653660834432335 0.8399360942898143 >500 3.9788822437688165E-4 0.17394328413040153 >1k 2.2104901224886874E-4 0.3158452352951925 >5k 8.841960489954748E-5 0.4801016044557859 >10k+ 4.420980244977374E-5 0.34808095271428324 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10744 0.31728687825031526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8988 0.26542949196889737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5831 0.1721984165187629 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.953154116253865E-5 2 0.0 0.0 0.0 0.0 2.953154116253865E-5 3 0.0 0.0 0.0 0.0 2.953154116253865E-5 4 0.0 0.0 0.0 0.0 5.90630823250773E-5 5 0.0 2.953154116253865E-5 0.0 0.0 5.90630823250773E-5 6 0.0 2.953154116253865E-5 0.0 0.0 5.90630823250773E-5 7 0.0 2.953154116253865E-5 0.0 0.0 5.90630823250773E-5 8 0.0 2.953154116253865E-5 0.0 0.0 5.90630823250773E-5 9 0.0 2.953154116253865E-5 0.0 0.0 5.90630823250773E-5 10-11 0.0 2.953154116253865E-5 0.0 2.953154116253865E-5 8.859462348761595E-5 12-13 0.0 2.953154116253865E-5 0.0 2.953154116253865E-5 8.859462348761595E-5 14-15 0.0 2.953154116253865E-5 0.0 7.382885290634662E-5 8.859462348761595E-5 16-17 0.0 2.953154116253865E-5 0.0 8.859462348761595E-5 8.859462348761595E-5 18-19 0.0 2.953154116253865E-5 0.0 1.181261646501546E-4 8.859462348761595E-5 20-21 0.0 2.953154116253865E-5 0.0 1.6242347639396255E-4 8.859462348761595E-5 22-23 0.0 2.953154116253865E-5 0.0 4.725046586006184E-4 1.181261646501546E-4 24-25 0.0 2.953154116253865E-5 0.0 9.154777760386982E-4 1.4765770581269324E-4 26-27 0.0 2.953154116253865E-5 0.0 0.002111505193121513 1.4765770581269324E-4 28-29 0.0 2.953154116253865E-5 0.0 0.005064659309375379 1.4765770581269324E-4 30-31 0.0 2.953154116253865E-5 0.0 0.018029005879729845 1.4765770581269324E-4 32-33 0.0 5.90630823250773E-5 0.0 0.04831360134191323 1.771892469752319E-4 34-35 0.0 5.90630823250773E-5 0.0 0.091887390327239 1.771892469752319E-4 36-37 0.0 5.90630823250773E-5 0.0 0.15867297066632016 1.771892469752319E-4 38-39 0.0 7.382885290634662E-5 0.0 0.259980922624409 1.9195501755650123E-4 40-41 0.0 8.859462348761595E-5 0.0 0.37321961721216346 2.0672078813777054E-4 42-43 0.0 8.859462348761595E-5 0.0 0.48309171610738844 2.0672078813777054E-4 44-45 0.0 1.181261646501546E-4 0.0 0.6078920090602769 2.2148655871903985E-4 46-47 0.0 1.181261646501546E-4 0.0 0.757351138883885 2.362523293003092E-4 48-49 0.0 1.181261646501546E-4 0.0 0.9143260459333591 2.6578387046284787E-4 50-51 0.0 1.181261646501546E-4 0.0 1.0761441257334896 2.6578387046284787E-4 52-53 0.0 1.181261646501546E-4 0.0 1.2333700508828453 3.248469527879251E-4 54-55 0.0 1.181261646501546E-4 0.0 1.4144574612915324 3.543784939504638E-4 56-57 0.0 1.4765770581269324E-4 0.0 1.6223152137640606 3.543784939504638E-4 58-59 0.0 1.4765770581269324E-4 0.0 1.8503873061623466 3.543784939504638E-4 60-61 0.0 1.6242347639396255E-4 0.0 2.076406956449836 3.543784939504638E-4 62-63 0.0 1.771892469752319E-4 0.0 2.3014668316495435 3.543784939504638E-4 64-65 0.0 1.771892469752319E-4 0.0 2.5352532772627807 3.543784939504638E-4 66-67 0.0 1.771892469752319E-4 0.0 2.801480120843067 3.543784939504638E-4 68-69 0.0 1.771892469752319E-4 0.0 3.0985231276264615 3.543784939504638E-4 70-71 0.0 1.771892469752319E-4 0.0 3.406935777757434 3.543784939504638E-4 72-73 0.0 1.771892469752319E-4 0.0 3.7071829567569643 4.725046586006184E-4 74-75 0.0 1.771892469752319E-4 0.0 4.00946781209671 4.725046586006184E-4 76-77 0.0 1.9195501755650123E-4 0.0 4.3433661822509535 4.725046586006184E-4 78-79 0.0 2.2148655871903985E-4 0.0 4.710147923489683 4.725046586006184E-4 80-81 0.0 2.362523293003092E-4 0.0 5.088284542305409 4.872704291818877E-4 82-83 0.0 2.362523293003092E-4 0.0 5.4749410107465275 5.020361997631571E-4 84-85 0.0 2.362523293003092E-4 0.0 5.860814893346839 5.020361997631571E-4 86-87 0.0 2.362523293003092E-4 0.0 6.269871035759744 5.020361997631571E-4 88 0.0 2.362523293003092E-4 0.0 6.600417575992038 5.020361997631571E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3315 0.0 49.47417 2 GGTATCA 5395 0.0 45.66212 1 GTACCTG 4070 0.0 41.58382 1 ACCTGGG 3925 0.0 40.235264 3 GTATCAA 9120 0.0 39.58974 1 TAACGCA 375 0.0 32.587368 4 ATCAACG 11280 0.0 31.12567 3 CAACGCA 11345 0.0 31.103865 5 CCTGGGG 3810 0.0 30.593834 4 TATCAAC 11755 0.0 30.582674 2 ATAACGC 415 0.0 29.446419 3 CTAACGC 580 0.0 29.173042 3 AACGCAG 12370 0.0 28.944374 6 TCAACGC 12185 0.0 28.891062 4 GTACATG 10400 0.0 27.755642 1 GTACTAG 1545 0.0 27.386108 1 TACATGG 10500 0.0 26.987625 2 TGGGGGG 5985 0.0 26.928297 6 GTGGTAT 2375 0.0 26.723076 1 CTGGGGG 2780 0.0 26.704914 5 >>END_MODULE