FastQCFastQC Report
Mon 6 Jun 2016
ERR1211215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527603
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278310.3697192851429598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209210.27792379592813277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139820.1857430579163115No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC81160.10781652539327592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145600.045.3500331
TACCTGG43750.042.382352
GTATCAA263800.040.0875551
ACCTGGG46950.038.049583
GTACTAG75200.037.362661
GCATCGT72600.034.835698
CAACGCA298600.033.628955
ATCAACG303150.033.124213
TAGGCAT89250.033.0790675
TATCAAC333100.031.379122
GGGCACC80500.030.94184394
CTGTGCG93700.030.8040289
GTACCTG61050.030.681651
CCTGGGG48150.030.0715774
CTAGGCA101600.029.7059294
AACGCAG347200.029.666576
GTACATG388050.029.641131
TAGTACT97950.029.5170214
ACTGTGC102200.029.2079818
TCTAACG35950.029.1926042
Produced by FastQC (version 0.11.5)