FastQCFastQC Report
Mon 6 Jun 2016
ERR1211215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527603
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261690.34764054374280895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216050.28701035376068584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141100.18744346639959625No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC81540.10832133416175109No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT81270.1079626542473082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153700.047.298331
TACCTGG44150.040.9846082
GTATCAA275650.039.6449851
ACCTGGG45500.039.048893
GTACTAG73400.036.708671
GGGCACC82950.033.3812294
CAACGCA320750.032.49115
ATCAACG324200.032.1425973
TAGGCAT89850.031.9516075
TACCCCG4700.030.9908455
CCTGGGG44950.030.6384094
TATCAAC356750.030.4457572
CTGTGCG101400.029.7484119
AACGCAG367850.029.1994766
GTACCTG63300.029.1943631
ACTGTGC109700.028.6540628
CTAGGCA103500.028.1111494
TAGTACT108250.028.006584
GTACATG400950.027.8420121
TCAACGC385500.027.5069854
Produced by FastQC (version 0.11.5)