##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7527603 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.156025364249416 33.0 31.0 34.0 30.0 34.0 2 32.295653344099044 34.0 31.0 34.0 30.0 34.0 3 32.37198760349078 34.0 31.0 34.0 30.0 34.0 4 35.98886856812189 37.0 35.0 37.0 35.0 37.0 5 35.88077280377299 37.0 35.0 37.0 35.0 37.0 6 35.9086786324943 37.0 35.0 37.0 35.0 37.0 7 35.8473759840948 37.0 35.0 37.0 35.0 37.0 8 35.835243569566565 37.0 35.0 37.0 35.0 37.0 9 37.43060626337494 39.0 37.0 39.0 34.0 39.0 10-11 37.40625767591622 39.0 37.0 39.0 34.0 39.0 12-13 37.29363909069062 39.0 37.0 39.0 34.0 39.0 14-15 38.49102609422947 40.0 38.0 41.0 34.0 41.0 16-17 38.45034023446773 40.0 38.0 41.0 34.0 41.0 18-19 38.48573742531321 40.0 38.0 41.0 34.0 41.0 20-21 38.45071385937861 40.0 38.0 41.0 34.0 41.0 22-23 38.312989805652606 40.0 38.0 41.0 34.0 41.0 24-25 38.24208370181052 40.0 38.0 41.0 34.0 41.0 26-27 38.0951015216929 40.0 37.0 41.0 33.0 41.0 28-29 38.07860636380532 40.0 37.0 41.0 33.0 41.0 30-31 38.08006672243475 40.0 37.0 41.0 33.5 41.0 32-33 37.93690946507142 40.0 37.0 41.0 33.0 41.0 34-35 37.79578080831308 40.0 37.0 41.0 33.0 41.0 36-37 37.65965892462714 40.0 36.5 41.0 33.0 41.0 38-39 37.55894200318481 40.0 36.0 41.0 33.0 41.0 40-41 37.44927196612255 40.0 36.0 41.0 32.0 41.0 42-43 37.29203041127435 39.5 36.0 41.0 32.0 41.0 44-45 37.115079195860886 39.0 36.0 41.0 31.0 41.0 46-47 36.84636776142419 39.0 35.0 41.0 31.0 41.0 48-49 36.566384212876265 39.0 35.0 41.0 30.0 41.0 50-51 36.346122264949415 39.0 35.0 40.5 30.0 41.0 52-53 36.12527161700743 38.5 35.0 40.0 29.5 41.0 54-55 35.87038749253912 38.0 35.0 40.0 29.0 41.0 56-57 35.58272633400034 38.0 34.5 40.0 28.5 41.0 58-59 35.28415273759788 37.0 34.0 40.0 28.0 41.0 60-61 35.00803130823982 37.0 34.0 40.0 28.0 41.0 62-63 34.686395589671775 36.0 34.0 39.0 27.0 41.0 64-65 34.31038392965198 36.0 33.0 39.0 26.0 40.5 66-67 34.01319277863086 35.0 33.0 39.0 26.0 40.0 68-69 33.698342154866566 35.0 33.0 38.0 26.0 40.0 70-71 33.42401412508072 35.0 33.0 37.5 26.0 40.0 72-73 33.11612574414458 35.0 32.0 37.0 25.5 39.5 74-75 32.79868027046591 35.0 32.0 36.5 25.0 39.0 76-77 31.83601951378148 34.0 30.5 35.0 24.0 38.0 78-79 32.34871346164243 35.0 32.0 36.0 25.0 38.0 80-81 32.30735261941949 35.0 32.0 35.0 25.0 37.0 82-83 32.08345398927122 34.5 32.0 35.0 25.0 37.0 84-85 31.850667868642915 34.0 31.5 35.0 24.0 36.5 86-87 31.63863543281972 34.0 31.0 35.0 24.0 36.0 88-89 31.446290339700433 34.0 31.0 35.0 24.0 36.0 90-91 31.24257735164833 34.0 31.0 35.0 23.0 35.0 92-93 31.060157197450504 34.0 31.0 35.0 23.0 35.0 94-95 30.882745941835665 34.0 30.5 35.0 20.5 35.0 96-97 30.72680480094394 34.0 30.0 35.0 20.0 35.0 98-99 30.565051916260728 34.0 30.0 35.0 20.0 35.0 100 28.737644240802815 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3538284746694984 1101 2 -0.3401661046498319 1101 3 -0.37217964200263154 1101 4 -0.23870787049465036 1101 5 -0.25870879266983593 1101 6 -0.34995460254491206 1101 7 -0.33098833851734355 1101 8 -0.33893224532516797 1101 9 -0.4683492734936294 1101 10-11 -0.4376396474798412 1101 12-13 -0.45233766699940503 1101 14-15 -0.7094031467587385 1101 16-17 -0.6056303241755572 1101 18-19 -0.6633168060987416 1101 20-21 -0.7315925909924275 1101 22-23 -0.7194130836193011 1101 24-25 -0.6587077433814912 1101 26-27 -0.750272005623323 1101 28-29 -0.7953684076138785 1101 30-31 -0.7148072867021327 1101 32-33 -0.6543415755256774 1101 34-35 -0.6567923433670586 1101 36-37 -0.6354017000289005 1101 38-39 -0.7558772954895048 1101 40-41 -0.7200088665779347 1101 42-43 -0.7182733244449295 1101 44-45 -0.790949250784692 1101 46-47 -0.7442282731705703 1101 48-49 -0.697970495566409 1101 50-51 -0.774163669967372 1101 52-53 -0.7208840505681167 1101 54-55 -0.6023828115134435 1101 56-57 -0.6582838221812111 1101 58-59 -0.7664374793932396 1101 60-61 -0.8285407698395559 1101 62-63 -0.9383408423961086 1101 64-65 -0.7485014100828948 1101 66-67 -0.8075845624961602 1101 68-69 -0.9432504345624224 1101 70-71 -0.9479321330861055 1101 72-73 -0.9178891534358371 1101 74-75 -0.96627330182638 1101 76-77 -0.9508321659814314 1101 78-79 -0.8141817353634906 1101 80-81 -0.7440048474184948 1101 82-83 -0.7421831988090091 1101 84-85 -0.6876545710442592 1101 86-87 -0.8222145118517865 1101 88-89 -0.7874353775405112 1101 90-91 -0.8445375805803934 1101 92-93 -0.8879636164686637 1101 94-95 -1.0515500880436015 1101 96-97 -0.9385568277481582 1101 98-99 -1.0348572713545998 1101 100 -0.9892343741197749 1102 1 -0.3785853474754788 1102 2 -0.3223108888035817 1102 3 -0.3290675184990448 1102 4 -0.21862174924277156 1102 5 -0.27393604245994396 1102 6 -0.3242294667391832 1102 7 -0.3685339751200303 1102 8 -0.3918435327267886 1102 9 -0.5237036086394795 1102 10-11 -0.513403904483944 1102 12-13 -0.5342442063926711 1102 14-15 -0.6087432078933119 1102 16-17 -0.6729840100461502 1102 18-19 -0.6321015404046904 1102 20-21 -0.6831359892820998 1102 22-23 -0.7111467830868463 1102 24-25 -0.7490892310935706 1102 26-27 -0.8339729138867398 1102 28-29 -0.8059134073082603 1102 30-31 -0.7362920030896092 1102 32-33 -0.6563316811718565 1102 34-35 -0.6930728160456354 1102 36-37 -0.7770683574534871 1102 38-39 -0.8137482418429158 1102 40-41 -0.7704970795892692 1102 42-43 -0.7083099689055672 1102 44-45 -0.8242653282841488 1102 46-47 -0.8713093287237186 1102 48-49 -0.7600821542945511 1102 50-51 -0.7174330880798507 1102 52-53 -0.8188856186804259 1102 54-55 -0.8188824068463845 1102 56-57 -0.7725770032698094 1102 58-59 -0.8562542516494389 1102 60-61 -0.8907399911654394 1102 62-63 -0.7867589684585923 1102 64-65 -0.7447485644030962 1102 66-67 -0.8223344292480448 1102 68-69 -0.8981211383269496 1102 70-71 -0.8985298783382234 1102 72-73 -0.8773763594929918 1102 74-75 -1.0409777260069362 1102 76-77 -0.8431634422511252 1102 78-79 -0.695564464394927 1102 80-81 -0.7318833756947285 1102 82-83 -0.7661121243038806 1102 84-85 -0.823062087834483 1102 86-87 -0.7298782615996586 1102 88-89 -0.8506686410574318 1102 90-91 -0.9045389168804263 1102 92-93 -0.8999399701101503 1102 94-95 -0.9664904895015312 1102 96-97 -1.0177706681671097 1102 98-99 -1.0262316283177526 1102 100 -1.0099534069097444 1103 1 -0.38470935389141303 1103 2 -0.3754770450186591 1103 3 -0.40503020829076064 1103 4 -0.22069885164254543 1103 5 -0.2020631278617273 1103 6 -0.32913332341514945 1103 7 -0.3775632306038901 1103 8 -0.35134443769967305 1103 9 -0.5032638023623335 1103 10-11 -0.526255491899839 1103 12-13 -0.5718849772508321 1103 14-15 -0.7462141933295001 1103 16-17 -0.6902282143246552 1103 18-19 -0.7005861886230349 1103 20-21 -0.7017371154592027 1103 22-23 -0.7770967497204211 1103 24-25 -0.7660018388946597 1103 26-27 -0.7939644522546558 1103 28-29 -0.899307089910657 1103 30-31 -0.8030081742962878 1103 32-33 -0.6898360795110108 1103 34-35 -0.7892161503197173 1103 36-37 -0.7923832768586436 1103 38-39 -0.6642906881472257 1103 40-41 -0.7576635189506575 1103 42-43 -0.6647962152729505 1103 44-45 -0.7400268531632079 1103 46-47 -0.8121155341116619 1103 48-49 -0.8297275897988641 1103 50-51 -0.8642517406452939 1103 52-53 -0.8367963530684079 1103 54-55 -0.8590277225304703 1103 56-57 -0.8054804783226786 1103 58-59 -0.7849659635575748 1103 60-61 -0.9022888803279869 1103 62-63 -0.7744085786819923 1103 64-65 -0.9190142905653929 1103 66-67 -0.9065186193037107 1103 68-69 -0.8917920811581155 1103 70-71 -0.9388016476167707 1103 72-73 -0.9111025864885036 1103 74-75 -0.8878991445105981 1103 76-77 -0.7791508576975019 1103 78-79 -0.7355390528833183 1103 80-81 -0.6838858578826645 1103 82-83 -0.7749646641396275 1103 84-85 -0.9017280776524785 1103 86-87 -0.849151803971953 1103 88-89 -0.8902032983812944 1103 90-91 -0.8178983250616341 1103 92-93 -0.9358742469983774 1103 94-95 -0.9455172605453903 1103 96-97 -1.0596447630534698 1103 98-99 -1.077522631174709 1103 100 -1.2255422186789673 1104 1 -0.3970647915473684 1104 2 -0.37826273182602677 1104 3 -0.4386591962787101 1104 4 -0.2839860522289115 1104 5 -0.3235355720204751 1104 6 -0.3741412489111653 1104 7 -0.39976820424629267 1104 8 -0.41067988161698565 1104 9 -0.6213791953613423 1104 10-11 -0.6138770218324083 1104 12-13 -0.599178755350529 1104 14-15 -0.8151571167908855 1104 16-17 -0.7210194610203047 1104 18-19 -0.7649464649378714 1104 20-21 -0.7772739157570712 1104 22-23 -0.7508964862987924 1104 24-25 -0.7774738631595923 1104 26-27 -0.828346902668585 1104 28-29 -0.9610755715684078 1104 30-31 -0.9425195969521596 1104 32-33 -0.8432673839544194 1104 34-35 -0.8885186016969442 1104 36-37 -0.8289570790651837 1104 38-39 -0.8487816625911293 1104 40-41 -0.8901457336683123 1104 42-43 -0.8311086891453741 1104 44-45 -0.9321289281179546 1104 46-47 -0.9926811804980744 1104 48-49 -1.0225313814401318 1104 50-51 -0.9579404585303664 1104 52-53 -0.9355169118729947 1104 54-55 -0.9497018188479416 1104 56-57 -0.852661570503578 1104 58-59 -0.9611685116956252 1104 60-61 -0.91987326200654 1104 62-63 -0.9075223322425998 1104 64-65 -0.996894623294196 1104 66-67 -1.0243740486235424 1104 68-69 -1.0566599041382858 1104 70-71 -1.053900381328134 1104 72-73 -1.0619500001586388 1104 74-75 -1.1127750751466081 1104 76-77 -0.9950967889571771 1104 78-79 -0.8906843577602004 1104 80-81 -0.8104044378758353 1104 82-83 -0.75825542147372 1104 84-85 -0.7784405806704626 1104 86-87 -0.8735501053779196 1104 88-89 -0.8689928556217765 1104 90-91 -0.902766061593077 1104 92-93 -0.9581163627198812 1104 94-95 -1.0231011976304707 1104 96-97 -1.0172699732319472 1104 98-99 -1.1201468255806084 1104 100 -1.2733197144692205 1105 1 -0.3259441269297838 1105 2 -0.3407466199130056 1105 3 -0.3999592296111878 1105 4 -0.19316516340303735 1105 5 -0.1867989945895019 1105 6 -0.34832533705548485 1105 7 -0.361242145263418 1105 8 -0.3873629688500202 1105 9 -0.47628459753405394 1105 10-11 -0.4978894654029773 1105 12-13 -0.5271202009474862 1105 14-15 -0.7340101422078931 1105 16-17 -0.6583210458433513 1105 18-19 -0.5719188035600595 1105 20-21 -0.7376859018752739 1105 22-23 -0.6278715294113653 1105 24-25 -0.6797174346858625 1105 26-27 -0.6851192360025493 1105 28-29 -0.68066772025999 1105 30-31 -0.7079204517709883 1105 32-33 -0.7305861138724268 1105 34-35 -0.6514831249873083 1105 36-37 -0.530500179252293 1105 38-39 -0.6084233165733437 1105 40-41 -0.6509718565887468 1105 42-43 -0.6440374232210928 1105 44-45 -0.6597740144503277 1105 46-47 -0.6332992325446369 1105 48-49 -0.6151652469480524 1105 50-51 -0.7070096058330719 1105 52-53 -0.7650551310691327 1105 54-55 -0.6394479486056 1105 56-57 -0.6816691661866372 1105 58-59 -0.8085967058101673 1105 60-61 -0.6522674459572713 1105 62-63 -0.7553042441361768 1105 64-65 -0.6264500500446673 1105 66-67 -0.6557930441395854 1105 68-69 -0.665479155696076 1105 70-71 -0.8051715542712401 1105 72-73 -0.7517444526429955 1105 74-75 -0.7382944924090822 1105 76-77 -0.7664248707073256 1105 78-79 -0.6388874643639681 1105 80-81 -0.6583986283223418 1105 82-83 -0.6354637503047798 1105 84-85 -0.5799854505395032 1105 86-87 -0.6559012548279703 1105 88-89 -0.7253123377587123 1105 90-91 -0.7347327112083377 1105 92-93 -0.7364441611127184 1105 94-95 -0.6310349836276252 1105 96-97 -0.7119397224763446 1105 98-99 -0.7233309726449235 1105 100 -0.8479867384034421 1106 1 -0.44635775174119985 1106 2 -0.4253815466553448 1106 3 -0.46085320597837764 1106 4 -0.33778915465464365 1106 5 -0.34197603815933064 1106 6 -0.380169891079845 1106 7 -0.40418885499701673 1106 8 -0.43427916046411497 1106 9 -0.5406698305713888 1106 10-11 -0.5588410049783121 1106 12-13 -0.6022846889538869 1106 14-15 -0.7756193806724667 1106 16-17 -0.6425555614922374 1106 18-19 -0.7039133155637884 1106 20-21 -0.72071766542566 1106 22-23 -0.7405403966767921 1106 24-25 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0.24881174462385047 2310 5 0.3790919832130015 2310 6 0.4424764483870405 2310 7 0.462362086202603 2310 8 0.46273646859259543 2310 9 0.6472483678288299 2310 10-11 0.6937230384344488 2310 12-13 0.6896384076863029 2310 14-15 0.8991877407929749 2310 16-17 0.7161882516271731 2310 18-19 0.7836811227344356 2310 20-21 0.896692915235711 2310 22-23 0.9856298477556393 2310 24-25 0.9477704659876096 2310 26-27 0.9699439281499664 2310 28-29 0.9953157802401691 2310 30-31 0.8233455399483702 2310 32-33 0.907952514879284 2310 34-35 0.957688585298726 2310 36-37 0.8279447864428278 2310 38-39 0.9511617632775327 2310 40-41 1.0126810296293414 2310 42-43 1.0846382908558851 2310 44-45 1.0275543271326697 2310 46-47 0.7199100422284417 2310 48-49 0.9500370249575809 2310 50-51 1.0301661462699414 2310 52-53 1.110055509492689 2310 54-55 1.1554289529809196 2310 56-57 1.1311013936789536 2310 58-59 1.1397366693053783 2310 60-61 1.1673741014971526 2310 62-63 0.5625347427523977 2310 64-65 0.7281776168526832 2310 66-67 1.0761612736383626 2310 68-69 1.248275607370509 2310 70-71 1.1933299752531283 2310 72-73 1.2017090159431518 2310 74-75 1.2227772576080298 2310 76-77 1.1638929180396218 2310 78-79 1.08312085544501 2310 80-81 1.0416677758507475 2310 82-83 1.0444330636707733 2310 84-85 1.0985581177980848 2310 86-87 1.0293275348544704 2310 88-89 1.0897716820479104 2310 90-91 1.1842580044466615 2310 92-93 1.1811568106655521 2310 94-95 1.2420380439966685 2310 96-97 1.2717517941394298 2310 98-99 1.3239989963341046 2310 100 1.460562957147733 2311 1 0.35693346519416735 2311 2 0.29688862514038306 2311 3 0.3992263254522044 2311 4 0.26565603199608745 2311 5 0.3311222269062597 2311 6 0.42694190347049243 2311 7 0.47590882892757946 2311 8 0.5108812409802468 2311 9 0.6506868398171903 2311 10-11 0.6618049756807949 2311 12-13 0.7091056092563335 2311 14-15 0.8162720047284893 2311 16-17 0.6877344213347243 2311 18-19 0.7970248027147377 2311 20-21 0.7530314122578687 2311 22-23 0.901043865502686 2311 24-25 0.903927325140117 2311 26-27 0.287422547774014 2311 28-29 0.6364508381126441 2311 30-31 0.871561061780433 2311 32-33 0.9241553828078324 2311 34-35 0.9425647178409662 2311 36-37 0.9406446097365944 2311 38-39 0.939330214887768 2311 40-41 0.978831707084872 2311 42-43 0.9481786975659858 2311 44-45 1.0586931866140716 2311 46-47 0.6992745154418785 2311 48-49 0.9395594935604237 2311 50-51 1.073495792303909 2311 52-53 1.0126116832451615 2311 54-55 1.0258703086725447 2311 56-57 1.1289437397485003 2311 58-59 1.102307212584357 2311 60-61 1.1709244539543064 2311 62-63 1.1188865935790915 2311 64-65 1.1667459110096843 2311 66-67 1.1552205934345068 2311 68-69 1.1940748785001816 2311 70-71 1.2116736976912605 2311 72-73 1.2661177098047176 2311 74-75 1.2264537384024763 2311 76-77 1.1765229390252188 2311 78-79 0.9534917270536809 2311 80-81 0.8705893322307432 2311 82-83 1.0403480283091113 2311 84-85 1.1084945772035866 2311 86-87 1.11269812085256 2311 88-89 1.2006029525021802 2311 90-91 1.2634088535298105 2311 92-93 1.2725797081414747 2311 94-95 1.2431639113815827 2311 96-97 1.246947622722793 2311 98-99 1.3014977744467018 2311 100 1.420020055386825 2312 1 0.2567327638292909 2312 2 0.3550319434596574 2312 3 0.4517033258732823 2312 4 0.20669204446668488 2312 5 0.32458101055497224 2312 6 0.39499162849632796 2312 7 0.2926279171957731 2312 8 0.41063588023881437 2312 9 0.5725618531086738 2312 10-11 0.5979253731067189 2312 12-13 0.6668245305835185 2312 14-15 0.8490461122852793 2312 16-17 0.8280441606940911 2312 18-19 0.8238861417304477 2312 20-21 0.8171958246893425 2312 22-23 0.9092686871841735 2312 24-25 0.9044941004779332 2312 26-27 0.9314926332222839 2312 28-29 0.892790050533911 2312 30-31 0.9212433156328359 2312 32-33 0.7354388401240968 2312 34-35 0.8724561158070543 2312 36-37 0.9849370350810034 2312 38-39 0.8159529984003768 2312 40-41 0.8908553138186548 2312 42-43 0.9740069429203473 2312 44-45 0.7499644583004468 2312 46-47 0.7778220649180909 2312 48-49 0.956265609343042 2312 50-51 0.7191798888872469 2312 52-53 0.9571644621216961 2312 54-55 0.7741341222430336 2312 56-57 0.7971873343179183 2312 58-59 0.9247069203844447 2312 60-61 1.034637806682575 2312 62-63 1.0522083367408968 2312 64-65 1.017741682192053 2312 66-67 0.9832973320665701 2312 68-69 1.061969704605069 2312 70-71 0.9331816534402009 2312 72-73 0.9798257991064574 2312 74-75 1.0713280647902153 2312 76-77 1.0556533731143602 2312 78-79 0.9759189748026316 2312 80-81 0.9268089479049095 2312 82-83 0.8638604191594723 2312 84-85 0.9072195254199755 2312 86-87 0.9610804146857426 2312 88-89 0.9401851427681365 2312 90-91 1.0604346985490167 2312 92-93 1.131505230124528 2312 94-95 1.14651923945787 2312 96-97 0.7871496479191862 2312 98-99 0.5805707893374148 2312 100 0.8574942699134596 2313 1 0.08407145988184084 2313 2 0.25338892086959675 2313 3 0.3012597582683938 2313 4 0.19666876714007486 2313 5 0.33893163363018886 2313 6 0.2590765229505507 2313 7 0.2743462730155741 2313 8 0.38973867852388366 2313 9 0.6000863010804096 2313 10-11 0.4044660203039996 2313 12-13 0.4478044739649718 2313 14-15 0.47571500508964704 2313 16-17 0.7615733989248099 2313 18-19 0.7051394985357788 2313 20-21 0.6797749801145088 2313 22-23 0.6497678069841868 2313 24-25 0.5251565800242375 2313 26-27 0.7730632843929826 2313 28-29 0.6562543661940623 2313 30-31 0.8526393985864686 2313 32-33 0.9612180491538638 2313 34-35 0.9536077060072188 2313 36-37 0.9800612851120292 2313 38-39 0.9866251963869743 2313 40-41 0.9677409063319473 2313 42-43 1.0616006010481769 2313 44-45 0.853974986806314 2313 46-47 0.8818058041619707 2313 48-49 0.9500293038051879 2313 50-51 0.8624835641393389 2313 52-53 0.8864589176961104 2313 54-55 0.8002034765788082 2313 56-57 0.5533624156373449 2313 58-59 0.6149912567876967 2313 60-61 0.5563530260077556 2313 62-63 0.9030040625565121 2313 64-65 0.9676673566557241 2313 66-67 0.9157443492940871 2313 68-69 1.0255586196647286 2313 70-71 0.9609339991627266 2313 72-73 0.9706629068360542 2313 74-75 1.0434274069345832 2313 76-77 1.0191180407502216 2313 78-79 0.9016992267233732 2313 80-81 0.8346565330387108 2313 82-83 0.8462709221666529 2313 84-85 0.8732995029039543 2313 86-87 0.8924995585661115 2313 88-89 0.8996783316471664 2313 90-91 0.7605552958543953 2313 92-93 0.9662090373544707 2313 94-95 1.061686752517474 2313 96-97 1.1457087128318513 2313 98-99 1.1943711114342896 2313 100 1.2534180523121705 2314 1 -0.3437694831488507 2314 2 0.07461065033697878 2314 3 0.28379821537308914 2314 4 0.23476954382243775 2314 5 0.35008581670472694 2314 6 0.41320521466732885 2314 7 0.45626056071564847 2314 8 0.5103347001169354 2314 9 0.5156509183922537 2314 10-11 0.5861634203747386 2314 12-13 0.5019124222131737 2314 14-15 0.7779756948321932 2314 16-17 0.8525758386550706 2314 18-19 0.8337806376525734 2314 20-21 0.853238115445869 2314 22-23 0.9486017415975141 2314 24-25 0.8083333480865704 2314 26-27 0.7470419307615117 2314 28-29 0.8535076638988457 2314 30-31 0.9279415908275581 2314 32-33 0.9376943361042933 2314 34-35 0.9525454614536102 2314 36-37 0.9403346463171118 2314 38-39 0.7126195946155534 2314 40-41 0.7500139969733439 2314 42-43 0.8892447663563559 2314 44-45 0.9513957227705916 2314 46-47 0.911743591803905 2314 48-49 0.9366869788589014 2314 50-51 0.879901748439913 2314 52-53 0.8792668305140694 2314 54-55 0.8669469497788143 2314 56-57 0.8243930410414393 2314 58-59 0.9346656757052259 2314 60-61 0.9775476662922742 2314 62-63 0.8964350492977928 2314 64-65 0.9608449502414658 2314 66-67 0.6815844504953503 2314 68-69 0.6882892249475603 2314 70-71 0.8678550165634817 2314 72-73 0.9801705360745459 2314 74-75 1.0024157483757605 2314 76-77 0.8684257211759601 2314 78-79 0.7884954953070675 2314 80-81 0.8162862581111412 2314 82-83 0.836244251866546 2314 84-85 0.9144041196142965 2314 86-87 0.8905540822103895 2314 88-89 0.8745152585903782 2314 90-91 0.9140323718163899 2314 92-93 0.7707046919600202 2314 94-95 0.8508186541333096 2314 96-97 0.8905591966086348 2314 98-99 1.1093227577350788 2314 100 1.2308867027103751 2315 1 0.4531029811038252 2315 2 0.47856485366637713 2315 3 0.45063558008764204 2315 4 0.33933515547678184 2315 5 0.3954391160254147 2315 6 0.4352825390883197 2315 7 0.3727799950994495 2315 8 0.28167476327831764 2315 9 0.4965793170194104 2315 10-11 0.5877905664746237 2315 12-13 0.4354690504584795 2315 14-15 0.6344567611020793 2315 16-17 0.7782410870804597 2315 18-19 0.7597899505834889 2315 20-21 0.48838038471274103 2315 22-23 0.8588471361638526 2315 24-25 0.8919745116758762 2315 26-27 0.8484303791811953 2315 28-29 0.8221405546573024 2315 30-31 0.9175064538264479 2315 32-33 0.8511243934112471 2315 34-35 0.9324360912735941 2315 36-37 0.8815760170901399 2315 38-39 0.9535529108463976 2315 40-41 0.9520164526421624 2315 42-43 1.008462358934274 2315 44-45 1.0228750497841688 2315 46-47 0.9736276278066143 2315 48-49 0.8879681775592161 2315 50-51 0.9093247402748119 2315 52-53 1.0045299561697618 2315 54-55 0.888264764200251 2315 56-57 0.8269982867707029 2315 58-59 0.8207079381102957 2315 60-61 0.9414873896544904 2315 62-63 1.047469974336714 2315 64-65 1.0360699782835212 2315 66-67 1.0329482819100662 2315 68-69 1.112992698737223 2315 70-71 1.0243592646944037 2315 72-73 1.1141567959681709 2315 74-75 0.5107125394000676 2315 76-77 0.5319254603646044 2315 78-79 0.7890854399966045 2315 80-81 0.8363668945754625 2315 82-83 0.7940305442424176 2315 84-85 0.8826993969225256 2315 86-87 0.8979663200744419 2315 88-89 0.9194463283244829 2315 90-91 0.9510183008806621 2315 92-93 1.0098411425970433 2315 94-95 1.0206728184689702 2315 96-97 1.1060544599737128 2315 98-99 1.1457605197853056 2315 100 1.0220439020134577 2316 1 0.30758485879135833 2316 2 0.1114962999970146 2316 3 0.183521434580733 2316 4 -0.006485561121955641 2316 5 0.28910775677177725 2316 6 0.40003150733439696 2316 7 0.4354517758028251 2316 8 0.43621218029620223 2316 9 0.6165041714773238 2316 10-11 0.6377078606620898 2316 12-13 0.3665124485823341 2316 14-15 0.5420651429379291 2316 16-17 0.7819492424700911 2316 18-19 0.737442196765933 2316 20-21 0.6981837079770656 2316 22-23 0.7900736757227733 2316 24-25 0.5003889759486597 2316 26-27 0.7419741107835591 2316 28-29 0.8854484672205487 2316 30-31 0.5668054653439896 2316 32-33 0.669043259795167 2316 34-35 0.7665986001559304 2316 36-37 0.8278069157142909 2316 38-39 0.7371147380641005 2316 40-41 0.6997972261869094 2316 42-43 0.7049306509051476 2316 44-45 0.550799638741033 2316 46-47 0.6828860443550084 2316 48-49 0.819242186362743 2316 50-51 0.8444011266774041 2316 52-53 0.8081192116259359 2316 54-55 0.7097127285902403 2316 56-57 0.7833281085360326 2316 58-59 0.5895920235682084 2316 60-61 0.7029173585826527 2316 62-63 0.7681737252716019 2316 64-65 0.8105165418689779 2316 66-67 0.7825702086974431 2316 68-69 0.7593311624811676 2316 70-71 0.7212148629758346 2316 72-73 0.8060144074356685 2316 74-75 0.8158565932579762 2316 76-77 0.7274678978630078 2316 78-79 0.4594692783819667 2316 80-81 0.4955420787828402 2316 82-83 0.4005792315836558 2316 84-85 0.5053041180031101 2316 86-87 0.5639151989840876 2316 88-89 0.5537272018153807 2316 90-91 0.46474529430336986 2316 92-93 0.3359792442829139 2316 94-95 0.3154402525339357 2316 96-97 0.5517154700531357 2316 98-99 0.7614183949468085 2316 100 0.4438752005026956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 30.0 13 191.0 14 681.0 15 1928.0 16 4765.0 17 9971.0 18 17188.0 19 25238.0 20 36317.0 21 52485.0 22 64202.0 23 63836.0 24 61465.0 25 64952.0 26 75316.0 27 93113.0 28 115128.0 29 142278.0 30 175946.0 31 219597.0 32 280301.0 33 373073.0 34 546942.0 35 750307.0 36 1175024.0 37 1684609.0 38 1281147.0 39 211547.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80800241139288 21.75410225188264 13.657010728731326 22.780884607993162 2 15.436255073494179 20.295809967661686 41.418138018171256 22.84979694067288 3 18.586965864166853 27.4508897453811 29.20390461611751 24.75823977433454 4 12.295894759474946 16.60164924024227 39.5852873333491 31.51716866693368 5 12.597609092828089 39.0826269663796 34.40181422957613 13.917949711216174 6 29.388212954376048 38.34253745847118 18.1577455665502 14.111504020602576 7 27.19165167062502 32.432220238691464 23.07651023401796 17.299617856665563 8 26.79865024762863 35.430667637493634 19.594537597160745 18.176144517716995 9 27.08468102208449 15.265834304864963 21.811851689468597 35.83763298358195 10-11 26.113674539969374 26.42664408478726 26.873607742844484 20.586073632398875 12-13 25.888714235375737 24.136184130338975 27.81468601722793 22.160415617057357 14-15 23.39306867414325 25.260372073782417 25.705394805484303 25.641164446590032 16-17 21.59278137244763 27.834202503712845 27.049647625820803 23.52336849801872 18-19 21.064095702177703 27.698936832880268 29.689437660301692 21.547529804640337 20-21 22.618131071612773 26.924017308299685 29.018694863337963 21.439156756749576 22-23 22.90023796419657 26.628323783812725 28.69464555981499 21.776792692175718 24-25 22.21236215313443 26.515717717676228 29.61400752116623 21.657912608023107 26-27 22.072091819308948 26.839229727214647 28.696217516230675 22.392460937245726 28-29 21.36487332922843 27.346546070220395 29.276514313687592 22.012066286863583 30-31 22.563693563083927 26.783757864093737 29.11066742885899 21.541881143963348 32-33 22.831949896666593 27.049862247588162 28.269574471237146 21.848613384508102 34-35 22.647716741890726 27.011403068865157 29.042831337324554 21.298048851919564 36-37 22.27715588433805 26.616412432092602 28.748107841757566 22.358323841811785 38-39 22.48276100329333 26.814079272477464 29.223871434522376 21.47928828970683 40-41 22.046859258756413 25.8360463306635 29.946429082649434 22.170665327930653 42-43 22.166652596211524 26.080805240081045 28.978118969893895 22.774423193813533 44-45 21.92731457068596 27.080725140408962 28.961932240991246 22.030028047913824 46-47 22.12600881685436 26.71309694434173 28.24368973356911 22.9172045052348 48-49 22.869189192205212 26.74267690141442 28.233139000129327 22.15499490625104 50-51 22.50986975182631 26.459942724549723 28.190356174648272 22.839831348975693 52-53 21.944692908699157 27.14965239177246 27.853132659097486 23.0525220404309 54-55 21.926157157791323 27.039573664181493 27.877933843786828 23.156335334240357 56-57 22.879928242442663 26.560073606759794 27.440371025468448 23.1196271253291 58-59 21.8097815079316 27.06666959837909 28.32887808259099 22.79467081109832 60-61 22.21376624230667 27.83317341368175 27.488192017833946 22.464868326177633 62-63 22.547877002751772 26.945820654298004 27.232475564987023 23.2738267779632 64-65 21.687324827674054 27.030178361566115 27.56977939208986 23.712717418669975 66-67 22.089523372135414 26.78359020644337 27.51698040976298 23.609906011658236 68-69 21.61326263691405 26.815654376763494 27.861399199447987 23.70968378687446 70-71 21.65483707568949 26.344896298297886 28.0917220066279 23.908544619384724 72-73 22.185037690455818 26.620367970701412 27.987818217146675 23.206776121696088 74-75 22.367390358942465 26.967532353194258 27.475542204419227 23.18953508344405 76-77 22.000879841445155 26.71292844016045 27.347129209985173 23.939062508409222 78-79 21.876743638366868 26.71987888076312 27.455874884200327 23.947502596669686 80-81 21.423339201575445 27.29232746385133 27.761459146460375 23.52287418811285 82-83 22.506907583437535 26.214629657709988 28.74084299163515 22.53761976721733 84-85 22.61703500488491 26.728261979828083 27.379392939238436 23.275310076048573 86-87 21.202552446165132 27.416206197497097 27.964648621942125 23.41659273439565 88-89 22.11181848292332 26.24719508601736 27.88164427864681 23.759342152412508 90-91 21.966548787420955 26.157503197293174 28.02383856601775 23.85210944926812 92-93 22.32352585090906 26.427376020797837 27.696368817652008 23.552729310641098 94-95 21.095288965499968 26.334416002197003 28.36983258015072 24.200462452152305 96-97 21.39739537313328 26.318570929245915 27.908437783177682 24.375595914443128 98-99 20.7218955919732 26.786814068172333 27.844961870696412 24.646328469158057 100 21.32252796589144 26.27902783054528 27.167329178465437 25.231115025097843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1882.0 1 1314.0 2 1257.5 3 1618.5 4 1165.5 5 955.5 6 1396.5 7 2651.5 8 4088.5 9 4722.0 10 4254.5 11 3622.5 12 3935.0 13 4720.0 14 5507.5 15 6617.5 16 7884.5 17 8895.0 18 9835.5 19 10738.0 20 11537.0 21 12257.0 22 13220.0 23 14777.0 24 16226.5 25 17877.0 26 21431.0 27 25671.0 28 30595.0 29 42244.0 30 61128.0 31 79395.5 32 106228.5 33 138670.0 34 164914.5 35 191355.5 36 218425.0 37 254665.0 38 267252.5 39 252249.5 40 231097.5 41 207321.0 42 198925.5 43 205377.5 44 222468.5 45 263102.5 46 299120.5 47 317233.5 48 333401.0 49 332393.5 50 321628.0 51 327212.5 52 328154.5 53 310245.5 54 293462.0 55 256654.0 56 222198.0 57 184490.0 58 145846.0 59 136450.0 60 110990.5 61 74089.0 62 54242.5 63 44850.5 64 32166.5 65 15875.0 66 8769.0 67 5646.5 68 4073.5 69 3120.5 70 2405.0 71 1893.5 72 1546.5 73 1488.0 74 1212.0 75 830.5 76 565.0 77 332.0 78 187.0 79 93.5 80 58.5 81 50.0 82 38.0 83 18.5 84 11.0 85 9.5 86 6.5 87 4.5 88 2.5 89 2.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04411762947647478 2 0.0 3 0.0 4 0.03242732115389189 5 0.0 6 0.0 7 8.103509178154055E-4 8 0.0 9 0.010999517376248455 10-11 4.450287827346899E-4 12-13 6.642220637831193E-6 14-15 1.3284441275662385E-4 16-17 2.988999287024037E-4 18-19 0.0 20-21 6.642220637831193E-6 22-23 0.0 24-25 6.243687399561321E-4 26-27 4.782398859238459E-4 28-29 2.3911994296192294E-4 30-31 0.002577181607478503 32-33 0.016439496078632203 34-35 0.027698060059756075 36-37 0.03275943218578344 38-39 0.023752581000884346 40-41 0.0013882241133067192 42-43 1.2620219211879267E-4 44-45 0.011158930671556405 46-47 0.017748013544284947 48-49 0.003181623685521141 50-51 0.01086667296349183 52-53 0.010767039653924363 54-55 0.01018252423779522 56-57 4.450287827346899E-4 58-59 0.0095183021740121 60-61 0.00512115211176785 62-63 0.0011291775084313028 64-65 0.03575507369344531 66-67 0.061048649882306495 68-69 0.06036450115660988 70-71 0.05255324968652039 72-73 0.04539293583893837 74-75 0.03521041160114315 76-77 0.03166346578054129 78-79 0.01508448306851464 80-81 0.0010494708607773286 82-83 0.03855144858197224 84-85 0.05213478978633704 86-87 0.05829877053824437 88-89 0.07058687871823209 90-91 0.04862769728956216 92-93 0.03950792835381993 94-95 0.013105101318440943 96-97 0.0040650390303526894 98-99 0.02343375441026845 100 0.03260001889047549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7527603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.2348865273702 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71375839235662 46.208445318516944 2 14.372488575539252 17.31450436927063 3 4.532476823695958 8.19039681489784 4 1.830744038045636 4.410986375693535 5 0.8748114110552954 2.634708303888216 6 0.4700832051501851 1.6989245112386329 7 0.27350621552182003 1.1532231099541101 8 0.17995532529917643 0.8671670879513508 9 0.12215495636429935 0.6622190941823486 >10 0.5459451852640123 5.790957017069306 >50 0.04147614536010322 1.7329407511225778 >100 0.03663241375908761 4.4722031932127795 >500 0.0040384931038012556 1.6350739184481544 >1k 0.0017479926581307381 1.7127619591444327 >5k 9.041341334009471E-5 0.39207869495806275 >10k+ 9.041341334009471E-5 1.1234094804511878 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26169 0.34764054374280895 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21605 0.28701035376068584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14110 0.18744346639959625 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 8154 0.10832133416175109 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 8127 0.1079626542473082 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3284441275662385E-5 0.0 3 0.0 0.0 0.0 1.3284441275662385E-5 0.0 4 0.0 0.0 0.0 1.3284441275662385E-5 0.0 5 0.0 0.0 0.0 1.3284441275662385E-5 0.0 6 0.0 0.0 0.0 2.656888255132477E-5 0.0 7 0.0 0.0 0.0 2.656888255132477E-5 0.0 8 0.0 0.0 0.0 2.656888255132477E-5 0.0 9 0.0 0.0 0.0 2.656888255132477E-5 0.0 10-11 1.992666191349358E-5 0.0 0.0 4.6495544464818345E-5 0.0 12-13 2.656888255132477E-5 0.0 0.0 6.642220637831192E-5 0.0 14-15 2.656888255132477E-5 0.0 0.0 7.970664765397431E-5 0.0 16-17 3.985332382698716E-5 0.0 0.0 1.062755302052991E-4 0.0 18-19 3.985332382698716E-5 0.0 0.0 1.4612885403228622E-4 0.0 20-21 3.985332382698716E-5 0.0 0.0 2.590466048754165E-4 0.0 22-23 3.985332382698716E-5 0.0 0.0 5.845154161291449E-4 0.0 24-25 3.985332382698716E-5 0.0 0.0 0.0012221685973609394 0.0 26-27 3.985332382698716E-5 0.0 0.0 0.0025041171804623596 0.0 28-29 3.985332382698716E-5 0.0 0.0 0.006416385136144932 0.0 30-31 3.985332382698716E-5 0.0 0.0 0.021866190339740285 0.0 32-33 3.985332382698716E-5 0.0 0.0 0.05703674861705646 0.0 34-35 3.985332382698716E-5 0.0 0.0 0.10798258090922169 0.0 36-37 3.985332382698716E-5 0.0 0.0 0.18819403733167117 0.0 38-39 3.985332382698716E-5 0.0 0.0 0.30960718837058754 0.0 40-41 3.985332382698716E-5 0.0 0.0 0.4409637437043372 0.0 42-43 4.6495544464818345E-5 0.0 0.0 0.5671393669405785 0.0 44-45 5.313776510264954E-5 0.0 0.0 0.7064865137016392 0.0 46-47 5.313776510264954E-5 0.0 0.0 0.871439421021539 0.0 48-49 5.313776510264954E-5 0.0 0.0 1.052001546840342 0.0 50-51 5.313776510264954E-5 1.3284441275662385E-5 0.0 1.245170607429749 0.0 52-53 5.313776510264954E-5 1.3284441275662385E-5 0.0 1.431086363082644 0.0 54-55 5.313776510264954E-5 1.3284441275662385E-5 0.0 1.6311633331353952 0.0 56-57 5.313776510264954E-5 1.992666191349358E-5 0.0 1.859502952002118 0.0 58-59 5.313776510264954E-5 2.656888255132477E-5 0.0 2.1257630084902193 0.0 60-61 5.313776510264954E-5 2.656888255132477E-5 0.0 2.3944541177317666 0.0 62-63 5.313776510264954E-5 2.656888255132477E-5 0.0 2.666931292736878 0.0 64-65 5.313776510264954E-5 2.656888255132477E-5 0.0 2.9429354869006774 0.0 66-67 5.313776510264954E-5 2.656888255132477E-5 0.0 3.2427786109336534 0.0 68-69 5.313776510264954E-5 2.656888255132477E-5 0.0 3.571694734698416 0.0 70-71 5.313776510264954E-5 2.656888255132477E-5 0.0 3.9274985676051193 0.0 72-73 5.313776510264954E-5 2.656888255132477E-5 0.0 4.280227052356507 0.0 74-75 5.313776510264954E-5 2.656888255132477E-5 0.0 4.61863490941273 0.0 76-77 5.313776510264954E-5 2.656888255132477E-5 0.0 4.998244461085421 0.0 78-79 5.313776510264954E-5 2.656888255132477E-5 0.0 5.4077174367458 0.0 80-81 5.313776510264954E-5 2.656888255132477E-5 0.0 5.835057985921946 0.0 82-83 5.313776510264954E-5 2.656888255132477E-5 0.0 6.265566874342337 0.0 84-85 6.642220637831192E-5 2.656888255132477E-5 0.0 6.6902306086014365 0.0 86-87 6.642220637831192E-5 2.656888255132477E-5 0.0 7.135319171321867 0.0 88 6.642220637831192E-5 2.656888255132477E-5 0.0 7.483975974822264 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15370 0.0 47.29833 1 TACCTGG 4415 0.0 40.984608 2 GTATCAA 27565 0.0 39.644985 1 ACCTGGG 4550 0.0 39.04889 3 GTACTAG 7340 0.0 36.70867 1 GGGCACC 8295 0.0 33.38122 94 CAACGCA 32075 0.0 32.4911 5 ATCAACG 32420 0.0 32.142597 3 TAGGCAT 8985 0.0 31.951607 5 TACCCCG 470 0.0 30.990845 5 CCTGGGG 4495 0.0 30.638409 4 TATCAAC 35675 0.0 30.445757 2 CTGTGCG 10140 0.0 29.748411 9 AACGCAG 36785 0.0 29.199476 6 GTACCTG 6330 0.0 29.194363 1 ACTGTGC 10970 0.0 28.654062 8 CTAGGCA 10350 0.0 28.111149 4 TAGTACT 10825 0.0 28.00658 4 GTACATG 40095 0.0 27.842012 1 TCAACGC 38550 0.0 27.506985 4 >>END_MODULE