FastQCFastQC Report
Mon 6 Jun 2016
ERR1211214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699599
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168570.2957576489153009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132170.2318935068940815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81750.14343114313831554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86850.047.4068681
TACCTGG43300.041.5124632
GTATCAA155550.039.9609571
ACCTGGG51500.034.4117053
CAACGCA180250.033.3033335
ATCAACG180700.033.298443
GTACCTG55250.033.2150881
TATCAAC192700.032.0128252
AACGCAG200450.030.5100776
TCAACGC203400.029.8364644
GTACATG206250.028.4723741
GTGGTAT36200.028.2068271
GTACTAG33650.027.8272971
TACATGG214300.026.9417252
GCATCGT30600.026.8813198
CGCAGAG221600.026.8533138
ACGCAGA222850.026.6837067
ACATGGG216650.025.776853
TCTAACG15900.025.1550312
CCTGGGG50400.024.7165244
Produced by FastQC (version 0.11.5)