##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5699599 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99885904253966 31.0 31.0 34.0 27.0 34.0 2 31.009766125651996 31.0 31.0 34.0 27.0 34.0 3 31.062983729206213 31.0 31.0 34.0 27.0 34.0 4 34.64247028606749 37.0 35.0 37.0 31.0 37.0 5 34.69993994314337 37.0 35.0 37.0 31.0 37.0 6 34.76297964119932 37.0 35.0 37.0 31.0 37.0 7 34.66667690130481 37.0 35.0 37.0 30.0 37.0 8 34.66242449688127 37.0 35.0 37.0 30.0 37.0 9 36.16448174687377 39.0 35.0 39.0 30.0 39.0 10-11 36.096690311020126 39.0 35.0 39.0 30.0 39.0 12-13 35.987761595157835 39.0 35.0 39.0 30.0 39.0 14-15 37.10962832648402 40.0 36.0 41.0 30.0 41.0 16-17 37.08337332152666 40.0 36.0 41.0 30.0 41.0 18-19 37.095458031345714 40.0 36.0 41.0 30.0 41.0 20-21 37.062184023823434 40.0 36.0 41.0 30.0 41.0 22-23 36.95315986264998 40.0 36.0 41.0 30.0 41.0 24-25 36.852999658397025 40.0 36.0 41.0 30.0 41.0 26-27 36.70152689689222 39.0 36.0 41.0 29.5 41.0 28-29 36.58145564275662 39.0 36.0 41.0 29.0 41.0 30-31 36.424135890963555 39.0 35.0 41.0 29.0 41.0 32-33 36.23762145371981 39.0 35.0 40.5 28.5 41.0 34-35 36.08355307101429 39.0 35.0 40.0 27.5 41.0 36-37 35.97024492424818 39.0 35.0 40.0 27.0 41.0 38-39 35.831834485197994 38.0 35.0 40.0 27.0 41.0 40-41 35.671605493649636 38.0 34.5 40.0 26.5 41.0 42-43 35.47261956499045 38.0 34.0 40.0 26.0 41.0 44-45 35.265718868994114 38.0 34.0 40.0 25.0 41.0 46-47 35.04298346252079 38.0 33.0 40.0 24.0 41.0 48-49 34.85623725458581 38.0 33.0 40.0 24.0 41.0 50-51 34.21932130663929 37.5 32.5 39.5 23.5 40.5 52-53 34.363464605141516 37.5 33.0 39.5 23.5 40.5 54-55 34.86250690618761 38.0 33.0 40.0 24.0 41.0 56-57 34.84279999347322 38.0 33.0 40.0 24.0 41.0 58-59 34.64953499360218 38.0 33.0 40.0 24.0 41.0 60-61 34.445392561827596 37.0 33.0 40.0 24.0 41.0 62-63 34.16781671833404 37.0 33.0 40.0 23.0 41.0 64-65 33.93428844731007 36.0 32.0 40.0 23.0 41.0 66-67 33.763834876804495 36.0 32.0 39.0 23.0 41.0 68-69 33.47060433549799 35.5 32.0 39.0 22.0 41.0 70-71 33.161092297896744 35.0 32.0 39.0 22.0 40.5 72-73 32.81549280923097 35.0 31.0 38.0 21.5 40.0 74-75 32.47906475525734 35.0 31.0 37.0 21.0 39.5 76-77 32.13661241782097 35.0 31.0 37.0 20.0 39.0 78-79 31.784052790380517 35.0 30.5 36.0 20.0 39.0 80-81 31.469950517571498 35.0 30.0 36.0 20.0 38.5 82-83 31.174608687383095 34.0 30.0 35.5 19.5 37.0 84-85 30.908480754523257 34.0 30.0 35.0 19.0 37.0 86-87 30.669822298024826 34.0 29.0 35.0 18.0 36.5 88-89 30.443133718705475 34.0 29.0 35.0 18.0 36.0 90-91 30.209302794810654 34.0 29.0 35.0 18.0 36.0 92-93 30.01340550449251 34.0 29.0 35.0 17.0 35.5 94-95 29.83024402243035 34.0 29.0 35.0 17.0 35.0 96-97 29.64611589341636 34.0 29.0 35.0 15.5 35.0 98-99 29.403751298994894 34.0 27.5 35.0 14.5 35.0 100 28.26931157788469 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.38851279458607735 1101 2 -0.43018700896146456 1101 3 -0.3654946371251562 1101 4 -0.37659386069221057 1101 5 -0.3297525312130887 1101 6 -0.28457111622619635 1101 7 -0.3547431283632463 1101 8 -0.42730654009162095 1101 9 -0.5344983070936706 1101 10-11 -0.4823254491455202 1101 12-13 -0.531716702607504 1101 14-15 -0.5679769796672147 1101 16-17 -0.6698059089909449 1101 18-19 -0.5890243831790585 1101 20-21 -0.6787346895004305 1101 22-23 -0.7514475404471526 1101 24-25 -0.8409957791980389 1101 26-27 -0.852760314552576 1101 28-29 -0.8248342994668079 1101 30-31 -0.9055087843959342 1101 32-33 -0.9721380603733536 1101 34-35 -0.9680165191271399 1101 36-37 -1.0009275874732495 1101 38-39 -0.9928095091759772 1101 40-41 -1.0189431912203446 1101 42-43 -1.1190163440567602 1101 44-45 -1.012246161901082 1101 46-47 -1.2388777307705325 1101 48-49 -1.1001147862591338 1101 50-51 -1.1499806783833222 1101 52-53 -1.134869091564397 1101 54-55 -1.0552483660582865 1101 56-57 -0.9673898820707336 1101 58-59 -0.9321742993986462 1101 60-61 -0.9657960156951972 1101 62-63 -1.0180725305475917 1101 64-65 -0.879218816520769 1101 66-67 -0.9326221391849501 1101 68-69 -0.9226742992257826 1101 70-71 -1.028170623498987 1101 72-73 -1.0305827860623396 1101 74-75 -1.1320629069153014 1101 76-77 -1.1159738210289802 1101 78-79 -1.1034681598562166 1101 80-81 -1.2045133355468138 1101 82-83 -1.2037680635042491 1101 84-85 -1.2710977788225897 1101 86-87 -1.3174196107350014 1101 88-89 -1.2561801020670593 1101 90-91 -1.3371140704807694 1101 92-93 -1.4550622459405247 1101 94-95 -1.426063704669751 1101 96-97 -1.5612351397735154 1101 98-99 -1.6291072119138263 1101 100 -1.5970256844883082 1102 1 -0.4210662207134632 1102 2 -0.4626013294625757 1102 3 -0.45227041090280906 1102 4 -0.23568369637290942 1102 5 -0.28877439814232275 1102 6 -0.29455975058370143 1102 7 -0.3510914910375007 1102 8 -0.29365679738700834 1102 9 -0.43681543498976794 1102 10-11 -0.552320465604133 1102 12-13 -0.5410995792760005 1102 14-15 -0.6929829146877537 1102 16-17 -0.717554050688598 1102 18-19 -0.741618482174168 1102 20-21 -0.6927772920053741 1102 22-23 -0.6341544850777368 1102 24-25 -0.7060739307564745 1102 26-27 -0.7142719721001143 1102 28-29 -0.8602514381386968 1102 30-31 -0.8176477616298357 1102 32-33 -0.9242206533489181 1102 34-35 -0.8121952279626399 1102 36-37 -0.9025557110709101 1102 38-39 -0.9446602455458049 1102 40-41 -1.089670169228775 1102 42-43 -0.9532908115046084 1102 44-45 -1.0634265692258609 1102 46-47 -1.0072534610281707 1102 48-49 -1.164919457907125 1102 50-51 -1.1142800135151205 1102 52-53 -0.8551186938294393 1102 54-55 -0.6882552506821185 1102 56-57 -0.8440671584767827 1102 58-59 -0.8347458150934628 1102 60-61 -0.7455189424658997 1102 62-63 -0.963083309461986 1102 64-65 -0.892166623981403 1102 66-67 -0.9347118708594877 1102 68-69 -0.9583057656104259 1102 70-71 -0.937124761370292 1102 72-73 -1.0254885270978562 1102 74-75 -0.9512503541436388 1102 76-77 -1.1743246710969188 1102 78-79 -1.067713178347013 1102 80-81 -1.1117565409122818 1102 82-83 -1.0590077747631526 1102 84-85 -1.0863315957046389 1102 86-87 -1.09034101438872 1102 88-89 -1.1355303991724135 1102 90-91 -1.3956540908609725 1102 92-93 -1.172977962604417 1102 94-95 -1.2915060653551755 1102 96-97 -1.3247473838407338 1102 98-99 -1.5358793465182146 1102 100 -1.5037668465184346 1103 1 -0.5503726328111256 1103 2 -0.5019295943378559 1103 3 -0.6284698865700555 1103 4 -0.5253768142150506 1103 5 -0.4397552131089455 1103 6 -0.40196478394970114 1103 7 -0.5076378423798289 1103 8 -0.4553566784245575 1103 9 -0.6675512278781355 1103 10-11 -0.7516638779575899 1103 12-13 -0.7487379688777267 1103 14-15 -0.9017076415674197 1103 16-17 -0.9650558687995172 1103 18-19 -0.8469728360796651 1103 20-21 -0.752584132119722 1103 22-23 -0.9371062740732867 1103 24-25 -0.9940075819662866 1103 26-27 -0.9360801068000271 1103 28-29 -0.946089983586937 1103 30-31 -0.862063428753963 1103 32-33 -1.027295540220372 1103 34-35 -0.9647900803800766 1103 36-37 -0.9115826391989614 1103 38-39 -0.9655895823237515 1103 40-41 -1.106904974636258 1103 42-43 -1.178766853302342 1103 44-45 -1.168242411770862 1103 46-47 -1.1151164830839733 1103 48-49 -1.13525389382405 1103 50-51 -1.3058828912208966 1103 52-53 -1.1057264538990523 1103 54-55 -0.8896694485481191 1103 56-57 -0.8483835399072959 1103 58-59 -0.8117306280333381 1103 60-61 -0.9438129213039375 1103 62-63 -0.9672932528794931 1103 64-65 -0.9749876271454028 1103 66-67 -0.9982605634442621 1103 68-69 -0.8910826477072362 1103 70-71 -0.8760875726571768 1103 72-73 -1.0231617980413787 1103 74-75 -0.9788091741921079 1103 76-77 -0.9833635919631618 1103 78-79 -1.0375968330325627 1103 80-81 -1.1728869933496533 1103 82-83 -1.033416453015711 1103 84-85 -0.9950104193450251 1103 86-87 -0.9575027507402893 1103 88-89 -0.9940687266048904 1103 90-91 -1.2122316784616274 1103 92-93 -1.2181951148588261 1103 94-95 -1.281990684225363 1103 96-97 -1.427605062334134 1103 98-99 -1.4108154689410313 1103 100 -1.4875291122440366 1104 1 -0.48045074149497324 1104 2 -0.49482220973667523 1104 3 -0.5269051481084688 1104 4 -0.4187807011095117 1104 5 -0.42950926491906216 1104 6 -0.39129967667209087 1104 7 -0.41776119386451427 1104 8 -0.3989607284393273 1104 9 -0.5268962405078668 1104 10-11 -0.531432783534683 1104 12-13 -0.4988839784026098 1104 14-15 -0.7403467814998734 1104 16-17 -0.8455482634803033 1104 18-19 -0.7862167522273324 1104 20-21 -0.7055738282806772 1104 22-23 -0.7002681991859774 1104 24-25 -0.7843188515566624 1104 26-27 -0.7588058573109677 1104 28-29 -0.8353301258372241 1104 30-31 -0.8538326684858433 1104 32-33 -0.8980351581554018 1104 34-35 -0.9310221259716869 1104 36-37 -0.8820121380327208 1104 38-39 -1.0205965182341785 1104 40-41 -1.0266682523430788 1104 42-43 -0.8471156522869805 1104 44-45 -0.8981269891346599 1104 46-47 -1.0233290700210134 1104 48-49 -1.0336935521361141 1104 50-51 -1.0953880856425613 1104 52-53 -0.9104558434729881 1104 54-55 -0.8154321238969473 1104 56-57 -0.7599754137902366 1104 58-59 -0.8381233610458523 1104 60-61 -0.9617400330560031 1104 62-63 -0.797356119414701 1104 64-65 -0.7513486667478801 1104 66-67 -0.8809568409158501 1104 68-69 -1.0889828951723146 1104 70-71 -0.9033365773375337 1104 72-73 -0.8454438408117255 1104 74-75 -0.8858390290179727 1104 76-77 -0.9565762485157805 1104 78-79 -0.895694627537619 1104 80-81 -0.9668648057642386 1104 82-83 -1.0194522999112579 1104 84-85 -1.0312460629630742 1104 86-87 -0.9943825216976911 1104 88-89 -1.0053431534304842 1104 90-91 -1.0604048556508694 1104 92-93 -1.2194434321769094 1104 94-95 -1.2145705571146372 1104 96-97 -1.3243574587589713 1104 98-99 -1.1759848876216026 1104 100 -1.0275401188340645 1105 1 -0.5530907264221803 1105 2 -0.47931969881252456 1105 3 -0.5405271833701057 1105 4 -0.39151344167633795 1105 5 -0.3603106591522689 1105 6 -0.33456438532518007 1105 7 -0.4293650693110749 1105 8 -0.475754426853058 1105 9 -0.6646601049397063 1105 10-11 -0.6751235884072528 1105 12-13 -0.632167672975676 1105 14-15 -0.9072489605327476 1105 16-17 -0.7377741814350429 1105 18-19 -0.7838565559911856 1105 20-21 -0.7712187336047123 1105 22-23 -0.7606503089419832 1105 24-25 -0.8655322112673858 1105 26-27 -0.6794381898979154 1105 28-29 -0.8078559778484973 1105 30-31 -0.8733497594370974 1105 32-33 -0.9901172324033851 1105 34-35 -0.9643257485142129 1105 36-37 -0.9432196280506204 1105 38-39 -0.9098314291380376 1105 40-41 -0.8689644616809602 1105 42-43 -0.9606666715120369 1105 44-45 -1.0734880477083948 1105 46-47 -1.142126580111814 1105 48-49 -1.1450760459584401 1105 50-51 -1.0846824181280539 1105 52-53 -1.1030379285907799 1105 54-55 -0.8272639028486566 1105 56-57 -0.7165549046487385 1105 58-59 -0.6944966880022392 1105 60-61 -0.7462375221318496 1105 62-63 -0.76815620927173 1105 64-65 -0.8067560306760768 1105 66-67 -0.7325083239191059 1105 68-69 -0.8275743138263394 1105 70-71 -1.0089149594762787 1105 72-73 -0.8944630223881376 1105 74-75 -0.7959620751711469 1105 76-77 -0.909315800824892 1105 78-79 -0.8825494142358785 1105 80-81 -0.938536291376245 1105 82-83 -1.068493221090705 1105 84-85 -1.0902444469859098 1105 86-87 -1.018221808058989 1105 88-89 -1.031239368565597 1105 90-91 -1.1127715737679296 1105 92-93 -1.1237156264999761 1105 94-95 -1.184256492316127 1105 96-97 -1.2424593015526533 1105 98-99 -1.0847767454156134 1105 100 -1.1303155414881836 1106 1 -0.5341496856293908 1106 2 -0.460986926801052 1106 3 -0.4734829001781229 1106 4 -0.3667254682523762 1106 5 -0.343938431316829 1106 6 -0.3570550214559347 1106 7 -0.42853125119977165 1106 8 -0.39422293669660746 1106 9 -0.5449250488585449 1106 10-11 -0.49470861846348413 1106 12-13 -0.5687408540376921 1106 14-15 -0.7266972321717091 1106 16-17 -0.6709003359720569 1106 18-19 -0.8124768268544997 1106 20-21 -0.7221571616993785 1106 22-23 -0.7748624681940939 1106 24-25 -0.7703340646501147 1106 26-27 -0.7764244017918145 1106 28-29 -0.8645510167838211 1106 30-31 -0.8422708988184198 1106 32-33 -0.9417828274555902 1106 34-35 -0.8636480799390469 1106 36-37 -0.8174932265319867 1106 38-39 -0.8868248871868829 1106 40-41 -0.9505893353835475 1106 42-43 -0.9526111925003775 1106 44-45 -0.9107587987570511 1106 46-47 -0.9509985272863233 1106 48-49 -1.0419198862164478 1106 50-51 -0.9040352650504744 1106 52-53 -1.0130554826562843 1106 54-55 -0.7859684740497883 1106 56-57 -0.8034893728728179 1106 58-59 -0.8170174313965006 1106 60-61 -0.7781236656465396 1106 62-63 -0.7581085479427188 1106 64-65 -0.7383007884401778 1106 66-67 -0.7736690354711016 1106 68-69 -0.8725300714577671 1106 70-71 -0.8307258858614688 1106 72-73 -0.8065396071018114 1106 74-75 -0.7430881072379378 1106 76-77 -0.7814283101377271 1106 78-79 -0.80435727886546 1106 80-81 -0.7227463073994294 1106 82-83 -0.7257960185147496 1106 84-85 -0.7450325224683958 1106 86-87 -0.8049577724609804 1106 88-89 -0.7770840559093166 1106 90-91 -0.9018926457906282 1106 92-93 -0.9495716423394569 1106 94-95 -1.0005843246952146 1106 96-97 -1.1150014772361914 1106 98-99 -0.9971191925748144 1106 100 -1.1117786524359978 1107 1 -0.6231658884875522 1107 2 -0.6209092540960839 1107 3 -0.5468864924956343 1107 4 -0.5075876064163509 1107 5 -0.44006441045559086 1107 6 -0.5084429836297275 1107 7 -0.48083449222236396 1107 8 -0.5440597897434927 1107 9 -0.7499792943861863 1107 10-11 -0.6989513231187061 1107 12-13 -0.6816969354082474 1107 14-15 -0.8095312036081381 1107 16-17 -0.864597518848349 1107 18-19 -0.8995341479037862 1107 20-21 -0.8962001017537631 1107 22-23 -0.893357049954723 1107 24-25 -0.8892185071103498 1107 26-27 -0.9492516900565775 1107 28-29 -0.9873370966662165 1107 30-31 -0.9661693795468054 1107 32-33 -1.0442441647212561 1107 34-35 -1.0026085355852459 1107 36-37 -1.139961003452946 1107 38-39 -1.096401899240945 1107 40-41 -1.112187001233984 1107 42-43 -1.0058565713261771 1107 44-45 -1.143326390334984 1107 46-47 -1.086725337988419 1107 48-49 -1.0131504826962825 1107 50-51 -1.015687722085616 1107 52-53 -0.865445018667387 1107 54-55 -0.8709377774576694 1107 56-57 -0.9114293776529578 1107 58-59 -0.899758848571615 1107 60-61 -0.859474568235477 1107 62-63 -0.8482983734677418 1107 64-65 -0.8140786654283332 1107 66-67 -0.7381387695422035 1107 68-69 -0.8347614029962998 1107 70-71 -0.8460921660586251 1107 72-73 -0.6932250696250932 1107 74-75 -0.7474286874382514 1107 76-77 -0.7239420304817195 1107 78-79 -0.7114760201563648 1107 80-81 -0.9106114534416143 1107 82-83 -0.7900579339063327 1107 84-85 -0.8729615239159649 1107 86-87 -0.8744757633606817 1107 88-89 -0.8883567356266617 1107 90-91 -1.0588067521531705 1107 92-93 -1.0779493632967458 1107 94-95 -1.0417045180652558 1107 96-97 -1.2131328588308605 1107 98-99 -1.0608611554070038 1107 100 -1.0093252880768766 1108 1 -0.6300405796878223 1108 2 -0.6379759548372022 1108 3 -0.588833847466244 1108 4 -0.4170023776915883 1108 5 -0.419560278928202 1108 6 -0.4081961142200612 1108 7 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1.1964981106407038 2202 94-95 1.12178225017853 2202 96-97 1.2240796511931968 2202 98-99 1.2104042816419174 2202 100 1.2072007744732431 2203 1 1.0697978777349029 2203 2 1.0555314740634856 2203 3 1.052956291234004 2203 4 0.8977127448887003 2203 5 0.9060378744789404 2203 6 0.8713366813239674 2203 7 -0.12622316336018713 2203 8 0.6998604843325182 2203 9 0.9855304463448746 2203 10-11 1.1590316342311198 2203 12-13 1.1778135456764502 2203 14-15 1.4440279520983879 2203 16-17 1.480407039407325 2203 18-19 1.5016744140681695 2203 20-21 1.4935802019363962 2203 22-23 1.5031616476035623 2203 24-25 1.5199511209708305 2203 26-27 1.4731616305020268 2203 28-29 1.5128141822583885 2203 30-31 1.5429196516061126 2203 32-33 1.5534998471243924 2203 34-35 1.519374450798047 2203 36-37 1.512340581774815 2203 38-39 1.489047713162151 2203 40-41 1.4294435042388258 2203 42-43 1.3970881634530272 2203 44-45 1.453595422515228 2203 46-47 1.5877385008177285 2203 48-49 1.5862044805763702 2203 50-51 1.508785450982927 2203 52-53 1.3613870226762828 2203 54-55 0.7706030726425013 2203 56-57 0.8877891718346049 2203 58-59 1.1522987102937563 2203 60-61 1.297924380752697 2203 62-63 1.2573765677394704 2203 64-65 1.3027614036713402 2203 66-67 1.2314564021908012 2203 68-69 1.2629723164822622 2203 70-71 1.321806507111539 2203 72-73 1.2497207829429158 2203 74-75 1.2034547721907032 2203 76-77 1.1450039091867197 2203 78-79 1.187031920499205 2203 80-81 1.1562000031678537 2203 82-83 1.1035628163186715 2203 84-85 1.0816823659890957 2203 86-87 1.067659053583256 2203 88-89 1.1060322984296072 2203 90-91 1.0780510273666195 2203 92-93 1.0873275870269765 2203 94-95 1.1563347642272284 2203 96-97 1.104377985172274 2203 98-99 1.0311772703979507 2203 100 1.1028020527470943 2204 1 1.0442399017355157 2204 2 1.0809408426510245 2204 3 1.1052006894254376 2204 4 0.899481138791927 2204 5 0.9210728974192577 2204 6 0.9664506675695748 2204 7 1.032196649393498 2204 8 1.0054583731567348 2204 9 1.2674659978630913 2204 10-11 1.287368416941895 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1.3474359582306938 2204 80-81 1.3026718878018642 2204 82-83 1.412406525222579 2204 84-85 1.3841336533320394 2204 86-87 1.4159241490192969 2204 88-89 1.3294118014840706 2204 90-91 1.364321647686193 2204 92-93 1.3521611839633003 2204 94-95 1.3941011845377886 2204 96-97 1.3489646184426967 2204 98-99 1.3877827057152672 2204 100 1.2253183539953376 2205 1 1.1014024646270464 2205 2 1.1179903884286304 2205 3 1.1369596084159852 2205 4 1.0369047026132492 2205 5 0.9384149831040602 2205 6 0.9847950326178321 2205 7 1.053312141461447 2205 8 1.039684740377517 2205 9 1.2381387340307413 2205 10-11 1.2479234736057023 2205 12-13 1.303246263325562 2205 14-15 1.5278995667927617 2205 16-17 1.5569481796772067 2205 18-19 1.530656556289948 2205 20-21 1.573264873273672 2205 22-23 1.5519027842375905 2205 24-25 1.5277604174214332 2205 26-27 1.5427993633546535 2205 28-29 1.484348861110412 2205 30-31 1.5831692223517635 2205 32-33 1.5899634027445018 2205 34-35 1.5812129368362733 2205 36-37 1.6159411634919962 2205 38-39 1.6288688728984368 2205 40-41 1.652499481708979 2205 42-43 1.6639957953630713 2205 44-45 1.7017310266434578 2205 46-47 1.6230089568218489 2205 48-49 1.7087365232399065 2205 50-51 1.6488089766101552 2205 52-53 1.5778728590811326 2205 54-55 1.546094576410276 2205 56-57 1.546058632256397 2205 58-59 1.4485538318411315 2205 60-61 1.5033459443992925 2205 62-63 1.49203277831257 2205 64-65 1.5422266264373903 2205 66-67 1.501555054863907 2205 68-69 1.4849282780285407 2205 70-71 1.4331505947951158 2205 72-73 1.4121557628903076 2205 74-75 1.411582203424942 2205 76-77 1.435319476999183 2205 78-79 1.4077322608112404 2205 80-81 1.4374878138608054 2205 82-83 1.413897952121701 2205 84-85 1.3915251060735692 2205 86-87 1.4192876220425923 2205 88-89 1.4333503229710374 2205 90-91 1.519144519654816 2205 92-93 1.5494323167284598 2205 94-95 1.505219028582193 2205 96-97 1.4918228859455454 2205 98-99 1.466135769653576 2205 100 1.5204588576948126 2206 1 1.017903490710335 2206 2 1.0396987077289452 2206 3 1.077913261851819 2206 4 0.9452165386174798 2206 5 0.8925387794980679 2206 6 0.942182138578886 2206 7 1.003785018096103 2206 8 0.9447335268129962 2206 9 1.2260899401025824 2206 10-11 1.2800688479204112 2206 12-13 1.3041958598424728 2206 14-15 1.5544412804826848 2206 16-17 1.5704894583899716 2206 18-19 1.550211891125322 2206 20-21 1.5730495355910676 2206 22-23 1.5567182481989974 2206 24-25 1.5968621982230644 2206 26-27 1.568028865626431 2206 28-29 1.6080670547206992 2206 30-31 1.5955142016155932 2206 32-33 1.6040442370194796 2206 34-35 1.6549646572272962 2206 36-37 1.6220595734775927 2206 38-39 1.6317539913024888 2206 40-41 1.6503989436283177 2206 42-43 1.7019355981458446 2206 44-45 1.7864222463992476 2206 46-47 1.695585603079671 2206 48-49 1.7798939924155164 2206 50-51 1.7061421352876636 2206 52-53 1.538787066520399 2206 54-55 1.5651207556154247 2206 56-57 1.5408639371387878 2206 58-59 1.5923152707322217 2206 60-61 1.5349586767429386 2206 62-63 1.4911774548154781 2206 64-65 1.5342278496584711 2206 66-67 1.5811525652052794 2206 68-69 1.4927718712768936 2206 70-71 1.500445848320517 2206 72-73 1.533207201213898 2206 74-75 1.4381630704951576 2206 76-77 1.3768009978918556 2206 78-79 1.371735519866288 2206 80-81 1.3794680942188648 2206 82-83 1.4800913564895666 2206 84-85 1.4933363156828072 2206 86-87 1.4647494710857387 2206 88-89 1.4407374064776945 2206 90-91 1.499940631611878 2206 92-93 1.4814585858221143 2206 94-95 1.4442346546927247 2206 96-97 1.450880327346244 2206 98-99 1.5402040025200563 2206 100 1.5919641336866697 2207 1 1.0807894946703023 2207 2 1.1184620345369893 2207 3 1.1245033149869812 2207 4 0.9956953780499802 2207 5 0.9935050441678612 2207 6 0.9752810926622146 2207 7 1.0734248074219224 2207 8 1.0739173655753191 2207 9 1.2956798944310037 2207 10-11 1.2803835713274268 2207 12-13 1.3700992142440853 2207 14-15 1.6232787636799912 2207 16-17 1.5701090932389619 2207 18-19 1.5519747808272442 2207 20-21 1.5292280662029043 2207 22-23 1.597423852034801 2207 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54-55 1.3371629470081814 2310 56-57 1.2957993971255846 2310 58-59 1.3360715124579983 2310 60-61 1.2790645093888955 2310 62-63 1.269768863475413 2310 64-65 1.328214851186253 2310 66-67 1.2598148320829807 2310 68-69 1.2528780133255921 2310 70-71 1.3089561399229055 2310 72-73 1.2760800306569777 2310 74-75 1.280236862033881 2310 76-77 1.2776491046327507 2310 78-79 1.3391051593037737 2310 80-81 1.2854953017512187 2310 82-83 1.3227355313904354 2310 84-85 1.3387681409610614 2310 86-87 1.2861604783355673 2310 88-89 1.2524925351296226 2310 90-91 1.3814189046262655 2310 92-93 1.3769619772305184 2310 94-95 1.4263299108737435 2310 96-97 1.507281704100695 2310 98-99 1.5335661980452713 2310 100 1.6731193930979842 2311 1 0.8361842095791339 2311 2 0.8011442622950646 2311 3 0.8619969482440695 2311 4 0.8122442792659115 2311 5 0.8009437150697636 2311 6 0.7613126902285003 2311 7 0.8456572421570172 2311 8 0.7980263688443472 2311 9 1.053469430746901 2311 10-11 1.0343755191340236 2311 12-13 1.1152279034822001 2311 14-15 1.3067735035927583 2311 16-17 1.3286599762483249 2311 18-19 1.294924944278904 2311 20-21 1.323880523071118 2311 22-23 1.3542309999191886 2311 24-25 1.3967732973166562 2311 26-27 1.3861468109240818 2311 28-29 1.4084468063878646 2311 30-31 1.4424175495649862 2311 32-33 1.4957060314121051 2311 34-35 1.5065218230586268 2311 36-37 1.569672437286755 2311 38-39 1.5402023316360527 2311 40-41 1.583935442027439 2311 42-43 1.6548653395732345 2311 44-45 1.710478667104077 2311 46-47 1.716126020935647 2311 48-49 1.6304300720809621 2311 50-51 1.5857968674352563 2311 52-53 1.3728662730109562 2311 54-55 1.3693748451256909 2311 56-57 1.3872224856140676 2311 58-59 1.2749639028993798 2311 60-61 1.3116321143086438 2311 62-63 1.2926556052377762 2311 64-65 1.3606232818958475 2311 66-67 1.360938563043959 2311 68-69 1.3993437384999154 2311 70-71 1.3928464722154033 2311 72-73 1.3094096319719952 2311 74-75 1.3783227835620337 2311 76-77 1.3939435817345895 2311 78-79 1.2984558935449506 2311 80-81 1.3808591265527852 2311 82-83 1.4737095079541263 2311 84-85 1.4281843853022984 2311 86-87 1.4759854302509723 2311 88-89 1.5255584366905133 2311 90-91 1.564504049191747 2311 92-93 1.556923009917039 2311 94-95 1.6457302675889913 2311 96-97 1.63508141687662 2311 98-99 1.678883450925305 2311 100 1.6707891697544497 2312 1 0.6971003410654326 2312 2 0.7163986720043845 2312 3 0.825054906951145 2312 4 0.5035540115872692 2312 5 0.5806696673369842 2312 6 0.7239907151033265 2312 7 0.5401731914166064 2312 8 0.651059426183842 2312 9 0.8996287375777499 2312 10-11 0.8713195507671614 2312 12-13 1.0010282635126302 2312 14-15 1.161823439017276 2312 16-17 1.1929700800998475 2312 18-19 1.2102525542783695 2312 20-21 1.2835601004113144 2312 22-23 1.3477021311401103 2312 24-25 1.3089682223914139 2312 26-27 1.2752212806089958 2312 28-29 1.3047278556952264 2312 30-31 1.2868356404306525 2312 32-33 1.356038661491901 2312 34-35 1.2982301762953696 2312 36-37 1.3559774591362626 2312 38-39 1.4023737043515823 2312 40-41 1.395962846800714 2312 42-43 1.5033254796931814 2312 44-45 1.5777120278598673 2312 46-47 1.6414274469038403 2312 48-49 1.655947560536994 2312 50-51 1.6156035390556553 2312 52-53 1.488822167071774 2312 54-55 1.4040690182941375 2312 56-57 1.3800548282002723 2312 58-59 1.3431341610243948 2312 60-61 1.2941829122139197 2312 62-63 1.3075060864578134 2312 64-65 1.3151604835659683 2312 66-67 1.3318685384521984 2312 68-69 1.3226193298521096 2312 70-71 1.293912409975242 2312 72-73 1.338165293384158 2312 74-75 1.4191756222294742 2312 76-77 1.3714778377011854 2312 78-79 1.3602329577489023 2312 80-81 1.3582039684755358 2312 82-83 1.3846493023741004 2312 84-85 1.372709069943955 2312 86-87 1.364817410152959 2312 88-89 1.2502944954630522 2312 90-91 1.3222173915479516 2312 92-93 1.3663136562026246 2312 94-95 1.4254437249426353 2312 96-97 1.4456301094442487 2312 98-99 1.1014037081657193 2312 100 1.434557237620627 2313 1 0.7026372193317592 2313 2 0.7223282360620367 2313 3 0.7037516704893463 2313 4 0.6342700637757801 2313 5 0.5201571656771193 2313 6 0.6060706757099226 2313 7 0.6243199068859866 2313 8 0.6345924705661972 2313 9 0.8199152006357764 2313 10-11 0.9153639238741889 2313 12-13 0.977021475984138 2313 14-15 1.1311489991261183 2313 16-17 1.0876216032046315 2313 18-19 1.0670432357063717 2313 20-21 1.0784282721798206 2313 22-23 1.147055467215317 2313 24-25 1.1481718381309491 2313 26-27 1.1207023163990115 2313 28-29 1.171491639244131 2313 30-31 1.244079021040811 2313 32-33 1.3159096106418176 2313 34-35 1.294117585707646 2313 36-37 1.3647349053916216 2313 38-39 1.4236866794037866 2313 40-41 1.4098391006307835 2313 42-43 1.3848193708529735 2313 44-45 1.5015234302104332 2313 46-47 1.4573843048407227 2313 48-49 1.4444381930891055 2313 50-51 1.468117451250805 2313 52-53 1.382036134341078 2313 54-55 1.2622246519449334 2313 56-57 1.226434536808732 2313 58-59 1.2460028807365688 2313 60-61 1.1951884082223927 2313 62-63 1.2239762380548385 2313 64-65 1.2591653212242022 2313 66-67 1.2465685371393675 2313 68-69 1.2534274520732538 2313 70-71 1.2271240485915413 2313 72-73 1.2521833540035985 2313 74-75 1.2019078316541467 2313 76-77 0.9893397899000931 2313 78-79 1.1530462761805786 2313 80-81 1.2426423358960932 2313 82-83 1.193752011251803 2313 84-85 1.2198022675008922 2313 86-87 1.3522047734010911 2313 88-89 1.3314660661825428 2313 90-91 1.42277244815768 2313 92-93 1.450740982967087 2313 94-95 1.5979759607529687 2313 96-97 1.5959814842426674 2313 98-99 1.5313400382301126 2313 100 1.6619442414555863 2314 1 0.6701071220727819 2314 2 0.6372946000348705 2314 3 0.6406154508635105 2314 4 0.5542698036375171 2314 5 0.5613917544906215 2314 6 0.5532766634766233 2314 7 0.6241188773417505 2314 8 0.6025149625418678 2314 9 0.7410184957853332 2314 10-11 0.8054572405606777 2314 12-13 0.8469325024942194 2314 14-15 1.0304955044263338 2314 16-17 0.9738443042771152 2314 18-19 0.9739725681417468 2314 20-21 0.9648734064422939 2314 22-23 1.0519901103647626 2314 24-25 1.0637579451475148 2314 26-27 1.1298287090024601 2314 28-29 1.1400421961207812 2314 30-31 1.1522021228310493 2314 32-33 1.1371843553046759 2314 34-35 1.180038913607369 2314 36-37 1.1531360355126736 2314 38-39 1.2383118108607931 2314 40-41 1.2519163277656915 2314 42-43 1.3384730223844699 2314 44-45 1.347024459820993 2314 46-47 1.2808536879897972 2314 48-49 1.314074223932856 2314 50-51 1.2965518491193961 2314 52-53 1.282242047895636 2314 54-55 1.172604003915282 2314 56-57 1.1585969705630603 2314 58-59 1.1111995109277544 2314 60-61 1.0616450510358533 2314 62-63 1.064106578448964 2314 64-65 1.1399333443614452 2314 66-67 1.1138702870205748 2314 68-69 1.075182757251909 2314 70-71 1.0833134342102184 2314 72-73 1.1151226643672274 2314 74-75 1.1171967585159948 2314 76-77 1.1461379782082375 2314 78-79 1.081952146840134 2314 80-81 1.1648177386980478 2314 82-83 1.0934657479190975 2314 84-85 1.2008800541256015 2314 86-87 1.222863996918754 2314 88-89 1.273622686830766 2314 90-91 1.3875259169869665 2314 92-93 1.3882245919567744 2314 94-95 1.3954894817921755 2314 96-97 1.423895434441551 2314 98-99 1.4741362545789798 2314 100 1.5805541455824432 2315 1 0.4340477721750524 2315 2 0.4926589300436568 2315 3 0.47961015362783144 2315 4 0.21706355637434172 2315 5 0.38207144708126606 2315 6 0.43239967359387776 2315 7 0.3905579283571612 2315 8 0.4151648348606827 2315 9 0.6002401887422835 2315 10-11 0.4594352749949522 2315 12-13 0.518648871394852 2315 14-15 0.7356849767180549 2315 16-17 0.6382076839164768 2315 18-19 0.583894365154201 2315 20-21 0.7283929725763159 2315 22-23 0.5360820865497544 2315 24-25 0.6469705357579798 2315 26-27 0.7556959077506278 2315 28-29 0.7978474176175538 2315 30-31 0.8187697739824245 2315 32-33 0.847738061435571 2315 34-35 0.908335997766244 2315 36-37 0.9559441346178019 2315 38-39 1.0190013490821173 2315 40-41 0.9816206190459056 2315 42-43 1.0700126785631596 2315 44-45 1.0436913494586761 2315 46-47 1.063884624897618 2315 48-49 1.0102828653918365 2315 50-51 0.9469645983883481 2315 52-53 0.995151460613485 2315 54-55 0.8663611431729308 2315 56-57 0.7929332496070813 2315 58-59 0.7792921235418291 2315 60-61 0.8879627295955643 2315 62-63 0.7934393772217518 2315 64-65 0.8783871999399153 2315 66-67 0.8632004657058445 2315 68-69 0.9015367344956147 2315 70-71 0.8173785683892589 2315 72-73 0.5881642183996902 2315 74-75 0.7661700731596568 2315 76-77 0.789823845101381 2315 78-79 0.7646287842292132 2315 80-81 0.5918672145416366 2315 82-83 0.5125821287447607 2315 84-85 0.8044056319286348 2315 86-87 0.9522189147012199 2315 88-89 0.9080844778978054 2315 90-91 1.0111152744735463 2315 92-93 1.081421810389795 2315 94-95 1.0663970856665692 2315 96-97 0.8962940198507425 2315 98-99 0.3537813588086678 2315 100 0.916314536227663 2316 1 0.08895665306486578 2316 2 0.03074695253199522 2316 3 0.12619566450650055 2316 4 0.02230373104683281 2316 5 -0.018060686817257476 2316 6 0.06042442099435874 2316 7 0.014464786745854497 2316 8 0.08228501916615727 2316 9 0.06564455043965012 2316 10-11 0.03456426538540569 2316 12-13 -0.16272046203226154 2316 14-15 -0.14248181462333775 2316 16-17 0.09798005130839016 2316 18-19 0.055946980020124215 2316 20-21 0.09260312088595413 2316 22-23 0.047833904989253995 2316 24-25 0.07595037681368666 2316 26-27 0.13212449896884237 2316 28-29 0.05269289554799883 2316 30-31 -0.11821088118859535 2316 32-33 0.21992813817978885 2316 34-35 0.07669717512190033 2316 36-37 0.10027399197713294 2316 38-39 0.32139851374439843 2316 40-41 0.33943122428309636 2316 42-43 0.25971859539126996 2316 44-45 0.21321658383650544 2316 46-47 0.09428735623317408 2316 48-49 -0.011617156133205242 2316 50-51 0.07493222984559367 2316 52-53 0.1239294777951585 2316 54-55 -0.15846873443271647 2316 56-57 -0.22483907942336856 2316 58-59 -0.15797549662009658 2316 60-61 -0.0676732074149271 2316 62-63 0.048302757164258026 2316 64-65 0.017637254818104964 2316 66-67 -0.04644424329055141 2316 68-69 -0.13727621292148484 2316 70-71 -0.36353714083944055 2316 72-73 -0.3443706638839217 2316 74-75 -0.05230672135721193 2316 76-77 0.06913572585693117 2316 78-79 0.024144236436168143 2316 80-81 0.24448634284151893 2316 82-83 0.22050717898760652 2316 84-85 0.27910827737715493 2316 86-87 0.3007499906760813 2316 88-89 0.2777496250246365 2316 90-91 0.09681966536824049 2316 92-93 0.09514423543044614 2316 94-95 0.2914021472473607 2316 96-97 0.27337443611514445 2316 98-99 0.15216325134971243 2316 100 0.08538795792021148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20.0 1 383.0 2 122.0 3 130.0 4 92.0 5 97.0 6 86.0 7 472.0 8 1364.0 9 2589.0 10 4603.0 11 8826.0 12 14325.0 13 18589.0 14 22003.0 15 25374.0 16 29080.0 17 33945.0 18 39315.0 19 45074.0 20 52287.0 21 60799.0 22 66138.0 23 68230.0 24 68569.0 25 71894.0 26 78368.0 27 89057.0 28 102389.0 29 120222.0 30 142507.0 31 173493.0 32 214997.0 33 275425.0 34 376229.0 35 509477.0 36 738117.0 37 1032418.0 38 964591.0 39 247555.0 40 348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.978802807332436 19.71784476247958 13.025536557011607 23.27781587317638 2 15.501604556920572 19.9277958170134 41.3898909927311 23.180708633334937 3 19.176896122820857 26.133090091278945 28.926138740625728 25.76387504527447 4 12.594064555708556 16.355628992528533 38.79733542179474 32.25297102996817 5 12.98614330783996 37.76630375079363 34.72369971329166 14.523853228074742 6 30.142077780457736 37.24087604566796 18.471006730683822 14.14603944319048 7 27.526851253509083 31.95982507207763 22.70130606084066 17.812017613572625 8 25.760301392687836 35.666907856649594 20.31600802843163 18.25678272223094 9 26.388009559073268 16.06680819805501 21.387452268261367 36.15772997461036 10-11 25.199823143923915 26.294879247810538 27.907435959003653 20.597861649261894 12-13 25.48352719530838 23.980136991290582 28.304620663029983 22.231715150371052 14-15 22.6956192896207 25.51168245004611 26.20706659027236 25.58563167006083 16-17 21.696799074715397 28.219478181459877 27.31268067029436 22.771042073530364 18-19 21.394940166536607 27.66144541250458 29.44891233181956 21.494702089139253 20-21 22.559648265223238 26.913803377079944 29.05598946508436 21.47055889261246 22-23 22.568777891392518 26.888739268035796 28.689942878393026 21.852539962178664 24-25 22.100907658234732 26.83450680743676 29.324582586018693 21.74000294830981 26-27 21.97077866554964 27.061124351986543 28.76190439422066 22.20619258824315 28-29 21.522505898221816 27.446006121393037 28.986567523134653 22.044920457250495 30-31 22.376804615688872 27.055034603803602 28.91486950157151 21.653291278936017 32-33 22.274334497984345 27.193254237445778 28.548024239271776 21.9843870252981 34-35 22.322184906953716 27.239087214364023 28.927320866090948 21.511407012591317 36-37 22.136424372415668 26.994751747808728 28.682551114564784 22.186272765210823 38-39 22.284504823196833 27.235948202293308 28.77552975844619 21.70401721606367 40-41 22.3490560367275 26.601464589805605 29.061389311416402 21.988090062050485 42-43 22.09370314230803 26.884340111627875 28.648310773485875 22.37364597257822 44-45 22.048392254908258 27.345313532847694 28.600299724392574 22.005994487851474 46-47 22.172296136156266 27.09523095681029 28.249611277498893 22.482861629534554 48-49 22.45035618713893 27.1012548781999 28.199918347086665 22.248470587574502 50-51 22.267520958319235 27.187381975634857 28.10352307556227 22.441573990483636 52-53 22.007073186186208 27.503036092065507 27.984519309264698 22.505371412483584 54-55 21.947267359611892 27.390748795068642 28.099973321257693 22.56201052406177 56-57 22.46466180761676 27.18420512444269 27.691792742281642 22.659340325658906 58-59 21.914541457432524 27.517021259976516 28.107140753930114 22.461296528660846 60-61 22.119781297517385 27.55134981507898 27.965108897154007 22.363759990249626 62-63 22.167356105433498 27.28970099399573 27.75478478577031 22.78815811480046 64-65 21.925365353581327 27.334520912904193 27.874512483844523 22.865601249669957 66-67 22.12181876849474 27.167517194772767 27.774381203616983 22.936282833115513 68-69 21.826434487733728 27.085511985438792 28.010462795730636 23.077590731096848 70-71 21.938803316459314 26.925752654298705 28.035837826533132 23.09960620270885 72-73 22.125735164793113 27.123890341304634 28.00524554758609 22.74512894631616 74-75 22.215615658515382 27.2337210165536 27.845949403276954 22.704713921654072 76-77 22.1122109894553 26.98641445119409 27.735299051945738 23.166075507404877 78-79 21.94659500959693 27.019573347957227 27.807499862901015 23.226331779544832 80-81 21.719541425668748 27.365842298473442 28.012829355046925 22.90178692081088 82-83 22.21591344320085 26.863219791982267 28.55002409883391 22.370842665982966 84-85 22.346296810044542 27.06167219145874 27.82025413166184 22.771776866834877 86-87 21.606532024941945 27.461101800595518 27.987216853650537 22.945149320812 88-89 22.094084014056598 26.624161001394302 28.1079196765414 23.1738353080077 90-91 22.153193795356117 26.76005817094938 28.079126740824623 23.00762129286988 92-93 22.215487450320943 26.88111377606464 28.04539478042504 22.858003993189378 94-95 21.54073362484958 26.872981947274017 28.353102257832358 23.23318217004405 96-97 21.838411059517703 26.669517865945778 28.14976939156261 23.3423016829739 98-99 21.54648660388162 26.879583012497775 28.04499814628346 23.52893223733714 100 22.00618260047603 26.529758371730345 27.713329376337985 23.750729651455636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1261.0 1 889.5 2 1060.0 3 1553.5 4 1162.5 5 877.5 6 1202.0 7 2069.5 8 3312.5 9 4014.5 10 3748.5 11 3371.5 12 3493.0 13 3883.5 14 4444.5 15 5086.5 16 5667.5 17 6227.5 18 6870.5 19 7600.0 20 8323.0 21 9175.5 22 10463.5 23 12296.0 24 14276.5 25 17037.0 26 21106.0 27 26063.5 28 32081.5 29 41487.5 30 54394.5 31 67713.0 32 83999.5 33 101633.0 34 118394.0 35 136163.0 36 152480.0 37 171852.5 38 182804.0 39 181744.0 40 178073.0 41 175051.0 42 179974.0 43 191265.0 44 204933.5 45 224594.5 46 239847.5 47 246626.5 48 251106.0 49 248421.0 50 240516.5 51 238386.0 52 231697.0 53 215563.5 54 197384.0 55 171956.0 56 150299.5 57 126110.0 58 102467.5 59 90038.0 60 73269.0 61 54644.0 62 42112.5 63 33646.5 64 24851.0 65 15454.0 66 10383.0 67 7490.0 68 5553.0 69 4263.0 70 3292.5 71 2513.0 72 1893.0 73 1449.5 74 1113.0 75 778.5 76 560.5 77 404.5 78 256.0 79 169.0 80 115.5 81 84.5 82 60.5 83 48.5 84 41.0 85 33.0 86 29.5 87 28.0 88 24.5 89 17.0 90 9.5 91 8.0 92 12.0 93 9.5 94 5.0 95 5.0 96 4.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059179601933399176 2 0.13483404709699753 3 0.01794863112299655 4 0.017299462646407228 5 0.01973823070710764 6 0.05265282697958225 7 0.05807426101380115 8 0.049915792321529984 9 0.04200295494472506 10-11 0.04054671214588956 12-13 0.0416783707064304 14-15 0.019194332794289563 16-17 0.018036356592805915 18-19 0.01810653696865341 20-21 0.021308516616695316 22-23 0.026186052738096138 24-25 0.024896488331898437 26-27 0.01974700325408858 28-29 0.01858902705260493 30-31 0.01663274907585604 32-33 0.02325602204646327 34-35 0.022913892714206737 36-37 0.02317706912363484 38-39 0.02105411275424815 40-41 0.024852625596993753 42-43 0.03208120430928562 44-45 0.04149414721983073 46-47 0.04795074179780016 48-49 0.04058180233381331 50-51 0.028475687500120625 52-53 0.04049407686400394 54-55 0.03823075974292227 56-57 0.03960804961892933 58-59 0.04443295045844453 60-61 0.02378237486531947 62-63 0.026072009627343955 64-65 0.02551933916754495 66-67 0.03271282769191306 68-69 0.03980981819949088 70-71 0.04173100598831602 72-73 0.0452137071397479 74-75 0.027405436768446343 76-77 0.02089620690859129 78-79 0.020378626636716024 80-81 0.024861398143974693 82-83 0.020887434361610353 84-85 0.013018459719710105 86-87 0.01483437694476401 88-89 0.018676752522414297 90-91 0.021440104821409366 92-93 0.02011545022728792 94-95 0.023615696472681674 96-97 0.02477367267416532 98-99 0.02783529157051224 100 0.029352942198214296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5699599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.64446049124238 #Duplication Level Percentage of deduplicated Percentage of total 1 81.32792226677226 56.64039269143052 2 12.306269326517612 17.141269758104876 3 3.3035475180410097 6.902213538034467 4 1.2307786460848509 3.428676591628848 5 0.5826717206875913 2.028992881539559 6 0.32075185142795815 1.3403153786560367 7 0.1922012251104036 0.9370025439998921 8 0.12942891295474596 0.7211205451761002 9 0.09797527301725158 0.6141091527691797 >10 0.46182513301224914 5.654971989630577 >50 0.031085200786734823 1.4922022986545203 >100 0.01461610694643711 1.7660256557999958 >500 6.95114697813756E-4 0.33340951185883694 >1k 1.4481496442730036E-4 0.227772288589205 >5k 2.8962992885457755E-5 0.1648989071215607 >10k+ 5.792598577091551E-5 0.6066262670059714 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16857 0.2957576489153009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13217 0.2318935068940815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8175 0.14343114313831554 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.7545093961873457E-5 0.0 0.0 0.0 3 0.0 1.7545093961873457E-5 0.0 0.0 0.0 4 0.0 1.7545093961873457E-5 0.0 0.0 0.0 5 0.0 1.7545093961873457E-5 0.0 0.0 0.0 6 0.0 1.7545093961873457E-5 0.0 0.0 0.0 7 0.0 1.7545093961873457E-5 0.0 0.0 0.0 8 0.0 1.7545093961873457E-5 0.0 3.5090187923746915E-5 0.0 9 0.0 1.7545093961873457E-5 0.0 5.2635281885620375E-5 1.7545093961873457E-5 10-11 0.0 1.7545093961873457E-5 0.0 6.14078288665571E-5 4.3862734904683645E-5 12-13 0.0 1.7545093961873457E-5 0.0 7.895292282843056E-5 7.018037584749383E-5 14-15 0.0 1.7545093961873457E-5 0.0 9.649801679030402E-5 7.018037584749383E-5 16-17 0.0 1.7545093961873457E-5 0.0 1.4036075169498766E-4 7.018037584749383E-5 18-19 0.0 1.7545093961873457E-5 0.0 1.8422348659967132E-4 7.018037584749383E-5 20-21 0.0 1.7545093961873457E-5 0.0 3.24584238294659E-4 7.018037584749383E-5 22-23 0.0 1.7545093961873457E-5 0.0 5.438979128180772E-4 7.895292282843056E-5 24-25 0.0 1.7545093961873457E-5 0.0 8.246194162080526E-4 8.772546980936729E-5 26-27 0.0 1.7545093961873457E-5 0.0 0.0014474702518545604 8.772546980936729E-5 28-29 0.0 1.7545093961873457E-5 0.0 0.004166959815944947 8.772546980936729E-5 30-31 0.0 1.7545093961873457E-5 0.0 0.014623835817221528 9.649801679030402E-5 32-33 0.0 1.7545093961873457E-5 0.0 0.04069584544456549 1.5790584565686112E-4 34-35 0.0 3.5090187923746915E-5 0.0 0.08202331427175841 1.7545093961873458E-4 36-37 0.0 5.263528188562037E-5 0.0 0.14736124418577518 1.8422348659967132E-4 38-39 0.0 7.018037584749383E-5 0.0 0.24224511233158685 2.105411275424815E-4 40-41 0.0 7.018037584749383E-5 0.0 0.34774376232433196 2.105411275424815E-4 42-43 0.0 7.018037584749383E-5 0.0 0.4483122409137906 2.1931367452341825E-4 44-45 0.0 7.018037584749383E-5 0.0 0.5646537589749735 2.2808622150435496E-4 46-47 0.0 7.018037584749383E-5 0.0 0.704751334260533 2.2808622150435496E-4 48-49 0.0 7.018037584749383E-5 0.0 0.8577445536080697 2.2808622150435496E-4 50-51 0.0 7.018037584749383E-5 0.0 1.0143959250466568 2.2808622150435496E-4 52-53 0.0 7.018037584749383E-5 0.0 1.1664592544142138 2.2808622150435496E-4 54-55 0.0 7.018037584749383E-5 0.0 1.3341728075957624 2.2808622150435496E-4 56-57 0.0 7.018037584749383E-5 0.0 1.527212703911275 2.2808622150435496E-4 58-59 0.0 7.018037584749383E-5 0.0 1.7475966291663676 2.456313154662284E-4 60-61 0.0 7.018037584749383E-5 0.0 1.9739020236335927 2.456313154662284E-4 62-63 0.0 7.895292282843056E-5 0.0 2.201119762986835 2.456313154662284E-4 64-65 0.0 8.772546980936729E-5 8.772546980936729E-6 2.429732337310046 2.631764094281019E-4 66-67 0.0 1.228156577331142E-4 1.7545093961873457E-5 2.6876978538314713 2.719489564090386E-4 68-69 0.0 1.228156577331142E-4 1.7545093961873457E-5 2.9754023046182723 2.807215033899753E-4 70-71 0.0 1.228156577331142E-4 1.7545093961873457E-5 3.2795552809943294 2.807215033899753E-4 72-73 1.7545093961873457E-5 1.228156577331142E-4 1.7545093961873457E-5 3.574198816443052 2.807215033899753E-4 74-75 1.7545093961873457E-5 1.228156577331142E-4 1.7545093961873457E-5 3.871184621935684 2.807215033899753E-4 76-77 1.7545093961873457E-5 1.228156577331142E-4 1.7545093961873457E-5 4.201076251153809 2.807215033899753E-4 78-79 1.7545093961873457E-5 1.228156577331142E-4 1.7545093961873457E-5 4.558706673925657 2.807215033899753E-4 80-81 1.7545093961873457E-5 1.3158820471405092E-4 1.7545093961873457E-5 4.935759515713299 2.807215033899753E-4 82-83 1.7545093961873457E-5 1.4036075169498766E-4 1.7545093961873457E-5 5.313014126081502 2.982665973518488E-4 84-85 1.7545093961873457E-5 1.4036075169498766E-4 1.7545093961873457E-5 5.69078631672158 3.1581169131372224E-4 86-87 1.7545093961873457E-5 1.4036075169498766E-4 3.5090187923746915E-5 6.0928672350458335 3.1581169131372224E-4 88 1.7545093961873457E-5 1.4036075169498766E-4 3.5090187923746915E-5 6.419434068958185 3.1581169131372224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8685 0.0 47.406868 1 TACCTGG 4330 0.0 41.512463 2 GTATCAA 15555 0.0 39.960957 1 ACCTGGG 5150 0.0 34.411705 3 CAACGCA 18025 0.0 33.303333 5 ATCAACG 18070 0.0 33.29844 3 GTACCTG 5525 0.0 33.215088 1 TATCAAC 19270 0.0 32.012825 2 AACGCAG 20045 0.0 30.510077 6 TCAACGC 20340 0.0 29.836464 4 GTACATG 20625 0.0 28.472374 1 GTGGTAT 3620 0.0 28.206827 1 GTACTAG 3365 0.0 27.827297 1 TACATGG 21430 0.0 26.941725 2 GCATCGT 3060 0.0 26.881319 8 CGCAGAG 22160 0.0 26.853313 8 ACGCAGA 22285 0.0 26.683706 7 ACATGGG 21665 0.0 25.77685 3 TCTAACG 1590 0.0 25.155031 2 CCTGGGG 5040 0.0 24.716524 4 >>END_MODULE