FastQCFastQC Report
Mon 6 Jun 2016
ERR1211214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699599
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161240.2828970950412476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134960.2367885881094442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83610.14669453061522397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92450.050.3004531
GTATCAA162250.040.137251
TACCTGG45250.038.1201552
ACCTGGG49750.034.298423
CAACGCA192250.032.7037735
GTACCTG54100.032.5055051
ATCAACG194650.032.2394333
TATCAAC207800.031.1455212
GTACTAG35400.030.2840631
AACGCAG215200.029.8274776
TCAACGC214400.029.335394
GTACATG214950.026.9060651
CTGTGCG46300.026.5903829
CGCAGAG238200.026.434278
ACGCAGA240550.026.3325337
CCTGGGG46800.026.3157634
GTGGTAT34400.025.9703451
TACATGG218450.025.9694292
ACATGGG213900.025.6460573
GGGCACC42550.025.07853194
Produced by FastQC (version 0.11.5)