##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211214_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5699599 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19122310183576 33.0 31.0 34.0 30.0 34.0 2 32.33899104129957 34.0 31.0 34.0 31.0 34.0 3 32.41849733639156 34.0 31.0 34.0 31.0 34.0 4 36.01761106351517 37.0 35.0 37.0 35.0 37.0 5 35.90924624697281 37.0 35.0 37.0 35.0 37.0 6 35.93743910755827 37.0 35.0 37.0 35.0 37.0 7 35.87494874639427 37.0 35.0 37.0 35.0 37.0 8 35.85940063502713 37.0 35.0 37.0 35.0 37.0 9 37.47792800861956 39.0 37.0 39.0 35.0 39.0 10-11 37.4564569367073 39.0 37.0 39.0 35.0 39.0 12-13 37.36590539088803 39.0 37.0 39.0 34.0 39.0 14-15 38.617609414276345 40.0 38.0 41.0 34.0 41.0 16-17 38.57391072600019 40.0 38.0 41.0 34.0 41.0 18-19 38.5941083749927 40.0 38.0 41.0 34.0 41.0 20-21 38.55603990736893 40.0 38.0 41.0 34.0 41.0 22-23 38.43540308712946 40.0 38.0 41.0 34.0 41.0 24-25 38.38280070229502 40.0 38.0 41.0 34.0 41.0 26-27 38.2551444408633 40.0 38.0 41.0 34.0 41.0 28-29 38.23427306026266 40.0 38.0 41.0 34.0 41.0 30-31 38.23838124401384 40.0 38.0 41.0 34.0 41.0 32-33 38.1143275167253 40.0 37.0 41.0 33.5 41.0 34-35 37.982601319847234 40.0 37.0 41.0 33.0 41.0 36-37 37.8490976294999 40.0 37.0 41.0 33.0 41.0 38-39 37.7676044402422 40.0 37.0 41.0 33.0 41.0 40-41 37.67095746560416 40.0 37.0 41.0 33.0 41.0 42-43 37.540850505447835 40.0 37.0 41.0 32.5 41.0 44-45 37.41611155451463 40.0 36.5 41.0 32.0 41.0 46-47 37.196123446579314 40.0 36.0 41.0 31.0 41.0 48-49 36.98300512018477 39.0 36.0 41.0 31.0 41.0 50-51 36.800128658174025 39.0 35.5 41.0 31.0 41.0 52-53 36.61039934563817 39.0 35.0 41.0 30.0 41.0 54-55 36.3924166419427 39.0 35.0 40.0 30.0 41.0 56-57 36.15514477422008 38.5 35.0 40.0 29.5 41.0 58-59 35.90214171207484 38.0 35.0 40.0 29.0 41.0 60-61 35.65641407404276 38.0 34.0 40.0 28.5 41.0 62-63 35.367896846778166 37.0 34.0 40.0 28.0 41.0 64-65 35.009006510808916 37.0 34.0 40.0 28.0 41.0 66-67 34.70626266865441 36.0 34.0 39.0 27.5 41.0 68-69 34.382186185379005 36.0 33.5 39.0 26.5 40.0 70-71 34.05794732225899 35.0 33.0 38.5 26.0 40.0 72-73 33.710101973840615 35.0 33.0 38.0 26.0 40.0 74-75 33.36384603548425 35.0 32.5 37.0 26.0 39.0 76-77 32.346848787081335 34.0 31.0 36.0 25.5 39.0 78-79 32.8156315909242 35.0 32.0 36.0 26.0 39.0 80-81 32.7264097877763 35.0 32.0 36.0 26.0 37.5 82-83 32.45892412782022 35.0 32.0 35.5 25.5 37.0 84-85 32.1952866333228 34.5 32.0 35.0 25.0 37.0 86-87 31.950652844173774 34.0 32.0 35.0 25.0 36.0 88-89 31.725680350494834 34.0 31.0 35.0 25.0 36.0 90-91 31.5055595139237 34.0 31.0 35.0 24.0 35.5 92-93 31.325900734420088 34.0 31.0 35.0 24.0 35.0 94-95 31.143163843631804 34.0 31.0 35.0 23.0 35.0 96-97 30.973016347290397 34.0 30.5 35.0 23.0 35.0 98-99 30.80125750951953 34.0 30.0 35.0 21.0 35.0 100 29.043761850614402 32.0 27.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3512005770083988 1101 2 -0.25866566842746863 1101 3 -0.33536654199741633 1101 4 -0.22229117866987735 1101 5 -0.27393802126423594 1101 6 -0.31554063812815514 1101 7 -0.2358558105366626 1101 8 -0.25507716386277934 1101 9 -0.4151614332816109 1101 10-11 -0.37692306598676595 1101 12-13 -0.44539863873809793 1101 14-15 -0.6174603404915189 1101 16-17 -0.5924203439518578 1101 18-19 -0.7242640771626014 1101 20-21 -0.6388890016094919 1101 22-23 -0.6752995942758488 1101 24-25 -0.7106242353018075 1101 26-27 -0.8112325990448923 1101 28-29 -0.7746545020573805 1101 30-31 -0.6909270329860959 1101 32-33 -0.6431180510292762 1101 34-35 -0.6191670184907281 1101 36-37 -0.5904941860615978 1101 38-39 -0.6281198502588268 1101 40-41 -0.6769223301702638 1101 42-43 -0.6403138979177214 1101 44-45 -0.720699355015654 1101 46-47 -0.7048128292600495 1101 48-49 -0.734665167476507 1101 50-51 -0.7181715483272484 1101 52-53 -0.756328315847199 1101 54-55 -0.6434582331156875 1101 56-57 -0.6395855275028381 1101 58-59 -0.6610492407320834 1101 60-61 -0.7273085511110864 1101 62-63 -0.8206444453958994 1101 64-65 -0.8083350964314278 1101 66-67 -0.8104919315766281 1101 68-69 -0.8601095103253371 1101 70-71 -0.8758419959576358 1101 72-73 -0.9642471211747434 1101 74-75 -0.9734360058513687 1101 76-77 -0.9070807866563193 1101 78-79 -0.7818226216072333 1101 80-81 -0.704555279086108 1101 82-83 -0.6510969948849628 1101 84-85 -0.5755260468357761 1101 86-87 -0.6638138905863151 1101 88-89 -0.760430147644108 1101 90-91 -0.8557491909405357 1101 92-93 -0.904627272792851 1101 94-95 -0.9361828300048565 1101 96-97 -1.0674380582703549 1101 98-99 -1.0725256064451543 1101 100 -0.8283386730799158 1102 1 -0.3793154623317143 1102 2 -0.34568597130422773 1102 3 -0.35947629412719806 1102 4 -0.25317366984863554 1102 5 -0.3220589802134839 1102 6 -0.314244083649875 1102 7 -0.35257201734258103 1102 8 -0.31187865571538254 1102 9 -0.4104227127907478 1102 10-11 -0.4935944813646316 1102 12-13 -0.48946029375322553 1102 14-15 -0.5605873782774324 1102 16-17 -0.6891806668753375 1102 18-19 -0.6374116222942874 1102 20-21 -0.6965752961203222 1102 22-23 -0.7398357049417328 1102 24-25 -0.7012763122046621 1102 26-27 -0.7780103862483045 1102 28-29 -0.7243464958553645 1102 30-31 -0.7347403238054397 1102 32-33 -0.7229143657097978 1102 34-35 -0.7438042214713505 1102 36-37 -0.8443392560760756 1102 38-39 -0.8860150533240656 1102 40-41 -0.8207277215241575 1102 42-43 -0.8578841432367668 1102 44-45 -0.8969894534004794 1102 46-47 -0.9129052792677967 1102 48-49 -0.8614286606076291 1102 50-51 -0.8802642647074705 1102 52-53 -0.9079644743892672 1102 54-55 -0.9938223484495481 1102 56-57 -0.9624470598782437 1102 58-59 -1.0218825030258145 1102 60-61 -1.0052253766181494 1102 62-63 -1.0360749568831622 1102 64-65 -1.027912097773502 1102 66-67 -1.1098154121853199 1102 68-69 -1.0150919991488436 1102 70-71 -1.1243381482509918 1102 72-73 -1.089706910707143 1102 74-75 -1.1042599075911923 1102 76-77 -0.9402898267295363 1102 78-79 -0.8360374241216348 1102 80-81 -0.8380696262036267 1102 82-83 -0.757822087026284 1102 84-85 -0.9044807558030037 1102 86-87 -0.8391486330397733 1102 88-89 -0.849852409088065 1102 90-91 -0.9679741035201346 1102 92-93 -1.0085296504068921 1102 94-95 -1.0112820058134027 1102 96-97 -1.160452471355704 1102 98-99 -1.1259462729891254 1102 100 -1.1346996603088364 1103 1 -0.30865216150874275 1103 2 -0.3269240511821643 1103 3 -0.3512250145571727 1103 4 -0.21303269511274436 1103 5 -0.27554913287572447 1103 6 -0.3274563001737434 1103 7 -0.4069485101578678 1103 8 -0.36872880339935676 1103 9 -0.5538750420502936 1103 10-11 -0.523519848723474 1103 12-13 -0.5182817187307691 1103 14-15 -0.711190215219716 1103 16-17 -0.6993794174643924 1103 18-19 -0.6991839914587317 1103 20-21 -0.7097595492809177 1103 22-23 -0.687492247098568 1103 24-25 -0.9130088729614698 1103 26-27 -0.829133873445123 1103 28-29 -0.8391639472783723 1103 30-31 -0.829758532484199 1103 32-33 -0.6732391838051655 1103 34-35 -0.8587005610867919 1103 36-37 -0.8312159512318971 1103 38-39 -0.7961741235067876 1103 40-41 -0.8896624335694625 1103 42-43 -0.7531131996130753 1103 44-45 -0.7855161839142042 1103 46-47 -0.8519465258193932 1103 48-49 -0.9180522065865802 1103 50-51 -0.8849411765990425 1103 52-53 -0.8458299993630121 1103 54-55 -0.9328410470779502 1103 56-57 -0.8876172376208658 1103 58-59 -0.758144604811271 1103 60-61 -0.8051556060575464 1103 62-63 -0.7292340548323253 1103 64-65 -0.7833073037896483 1103 66-67 -0.919968968465426 1103 68-69 -0.9281393499844839 1103 70-71 -0.9840754738073656 1103 72-73 -0.9152635539456284 1103 74-75 -0.8384600658008168 1103 76-77 -0.8119407265885954 1103 78-79 -0.7747472654334899 1103 80-81 -0.629184937070562 1103 82-83 -0.674149619143293 1103 84-85 -0.7627242076005629 1103 86-87 -0.8023312615683516 1103 88-89 -0.9891385123008405 1103 90-91 -0.8485728266972146 1103 92-93 -0.9643225072642103 1103 94-95 -0.9690408249560143 1103 96-97 -0.9448713050353277 1103 98-99 -1.0429551856219277 1103 100 -1.1686701053969628 1104 1 -0.3922047839277454 1104 2 -0.3449119849696842 1104 3 -0.45491339469049663 1104 4 -0.3434710461174504 1104 5 -0.33425265623029077 1104 6 -0.3614741378844286 1104 7 -0.3516348992855285 1104 8 -0.3552409674400536 1104 9 -0.5969113953076075 1104 10-11 -0.5256533831359249 1104 12-13 -0.5953776790484255 1104 14-15 -0.7741373579397219 1104 16-17 -0.756366367107411 1104 18-19 -0.8294695545791839 1104 20-21 -0.8737412528401833 1104 22-23 -0.7233831103830966 1104 24-25 -0.7601880129085785 1104 26-27 -0.8736145668456388 1104 28-29 -0.904338055850836 1104 30-31 -0.8513023569591311 1104 32-33 -0.7766674214976774 1104 34-35 -0.7780329549761547 1104 36-37 -0.8193127857747129 1104 38-39 -0.9102854219693057 1104 40-41 -0.8855642741321859 1104 42-43 -0.7587659849325448 1104 44-45 -0.8917026642749164 1104 46-47 -0.8189791506126483 1104 48-49 -0.8453434935735231 1104 50-51 -0.8392165626540873 1104 52-53 -0.7866860658480874 1104 54-55 -0.8696583105699958 1104 56-57 -0.8045009949863768 1104 58-59 -0.9158341588831647 1104 60-61 -0.9210006989284025 1104 62-63 -0.9234314860905997 1104 64-65 -0.8446243426798432 1104 66-67 -0.9980131662997209 1104 68-69 -0.9224810686979623 1104 70-71 -0.9960405604934195 1104 72-73 -1.0010577981292457 1104 74-75 -0.923672836669553 1104 76-77 -0.8737344490217396 1104 78-79 -0.6360620326572999 1104 80-81 -0.712060456347853 1104 82-83 -0.7132156857969143 1104 84-85 -0.7603484842396995 1104 86-87 -0.7811736203787909 1104 88-89 -0.8555363162589629 1104 90-91 -0.8575645952041491 1104 92-93 -0.8207487949876118 1104 94-95 -0.8994105862918147 1104 96-97 -0.9288263788021993 1104 98-99 -1.0236713028018087 1104 100 -0.9528749891865722 1105 1 -0.3538872842719556 1105 2 -0.354488280106537 1105 3 -0.3941932341072949 1105 4 -0.21800455046899003 1105 5 -0.23043228332362986 1105 6 -0.34418100832364473 1105 7 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-0.8402973980384516 1105 70-71 -0.9242763192417982 1105 72-73 -0.8618993381627291 1105 74-75 -0.8385641024217065 1105 76-77 -0.8146193957959476 1105 78-79 -0.8164177650282944 1105 80-81 -0.7942658666103952 1105 82-83 -0.7856505938021598 1105 84-85 -0.7917274272601986 1105 86-87 -0.8458098081220022 1105 88-89 -0.8774404156234397 1105 90-91 -0.7892522405877429 1105 92-93 -0.7681806232157626 1105 94-95 -0.7531889292992737 1105 96-97 -0.8591484147884891 1105 98-99 -0.9809493595711594 1105 100 -0.9220047528770863 1106 1 -0.4065423366790064 1106 2 -0.38309018043618437 1106 3 -0.4057340115891037 1106 4 -0.28256677044592493 1106 5 -0.3658955779767865 1106 6 -0.3213158174231836 1106 7 -0.38019151826051 1106 8 -0.38690210037233896 1106 9 -0.5418246829361735 1106 10-11 -0.5389050559945829 1106 12-13 -0.6090124886980917 1106 14-15 -0.7109234296606104 1106 16-17 -0.6324793693363375 1106 18-19 -0.5892145023694226 1106 20-21 -0.6172551398368142 1106 22-23 -0.6552509858020414 1106 24-25 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0.3554092814334098 2311 28-29 0.7011724129466828 2311 30-31 0.8778443806467351 2311 32-33 0.9376468351375564 2311 34-35 0.9959038114595771 2311 36-37 0.9621385281878432 2311 38-39 1.026924717748237 2311 40-41 1.0116906131532133 2311 42-43 1.001568151456489 2311 44-45 1.0055394935561779 2311 46-47 0.6890891871091966 2311 48-49 0.8745425218244023 2311 50-51 1.0071682575895125 2311 52-53 0.9276760146397791 2311 54-55 1.0025190360695504 2311 56-57 1.0443867689435393 2311 58-59 1.042613543096742 2311 60-61 1.1402171326797799 2311 62-63 1.0883666406759076 2311 64-65 1.1266015361828963 2311 66-67 1.1826108362650274 2311 68-69 1.2153820448794193 2311 70-71 1.1537113624792354 2311 72-73 1.180437553262621 2311 74-75 1.1923055751188656 2311 76-77 1.157724658784943 2311 78-79 0.8828672404908602 2311 80-81 0.7908023301532694 2311 82-83 0.8804965973014376 2311 84-85 1.027379703015285 2311 86-87 1.0915761391759666 2311 88-89 1.1072671280013537 2311 90-91 1.1685482596588557 2311 92-93 1.114834184553331 2311 94-95 1.1529994205915237 2311 96-97 1.2332648053435022 2311 98-99 1.2885282585034794 2311 100 1.3565101840636586 2312 1 0.2852613705270599 2312 2 0.3916693312493109 2312 3 0.4453263921732855 2312 4 0.2076354182732274 2312 5 0.34693502137963605 2312 6 0.4177025306755908 2312 7 0.30483973390176544 2312 8 0.4019041840586439 2312 9 0.6127087812658516 2312 10-11 0.6043161249160178 2312 12-13 0.6810534069366838 2312 14-15 0.880282911491534 2312 16-17 0.854501715353301 2312 18-19 0.8199303749155504 2312 20-21 0.8450846591960186 2312 22-23 0.9135659594731464 2312 24-25 0.9431473258313048 2312 26-27 0.9613483460619676 2312 28-29 0.916075417033511 2312 30-31 0.9604864024785584 2312 32-33 0.8912019144857837 2312 34-35 0.9837271311701343 2312 36-37 1.07573398932756 2312 38-39 0.8748994360045259 2312 40-41 1.0155781898918832 2312 42-43 1.0597767568029894 2312 44-45 0.7880402012326968 2312 46-47 0.8818601608721011 2312 48-49 1.0494333848359219 2312 50-51 0.8531405830145644 2312 52-53 1.0565984223322218 2312 54-55 0.9223086571087649 2312 56-57 0.9964598188524221 2312 58-59 1.1634860197876478 2312 60-61 1.1299982837156435 2312 62-63 1.144549411406409 2312 64-65 1.1523626201664072 2312 66-67 1.226041828958266 2312 68-69 1.285183276236566 2312 70-71 1.196422515461208 2312 72-73 1.1443440603482316 2312 74-75 1.2249913844354268 2312 76-77 1.157045510473644 2312 78-79 1.057074089914984 2312 80-81 1.0223767556083914 2312 82-83 1.0522898274909167 2312 84-85 1.1156559356993228 2312 86-87 1.1053005301318528 2312 88-89 1.0440209715694522 2312 90-91 1.1223071277302594 2312 92-93 1.1509480231676932 2312 94-95 1.1705171788492201 2312 96-97 0.8617414874021314 2312 98-99 0.6748827071350085 2312 100 0.9091774611013257 2313 1 0.08349073337790003 2313 2 0.24012634037647018 2313 3 0.24179706244441235 2313 4 0.16518794801466896 2313 5 0.2890466663018074 2313 6 0.24898389458616066 2313 7 0.2381788996284726 2313 8 0.34711072072965976 2313 9 0.5587089323485159 2313 10-11 0.3266391908696278 2313 12-13 0.4006976503106259 2313 14-15 0.43538452319057797 2313 16-17 0.6985257934531361 2313 18-19 0.6957200562279766 2313 20-21 0.6229913163672904 2313 22-23 0.5961079058956216 2313 24-25 0.5461462198287421 2313 26-27 0.7144862274999539 2313 28-29 0.5622780769922784 2313 30-31 0.7640790599472567 2313 32-33 0.8904694218349221 2313 34-35 0.8966634011888601 2313 36-37 0.9072610562599763 2313 38-39 0.9708600516383967 2313 40-41 0.9252436601646465 2313 42-43 0.9540655954314232 2313 44-45 0.7697865962863091 2313 46-47 0.8612736416207909 2313 48-49 0.9919268363064191 2313 50-51 0.9201969331888762 2313 52-53 0.8957411853328097 2313 54-55 0.8668696489858192 2313 56-57 0.5650831996907044 2313 58-59 0.6973935748461955 2313 60-61 0.5163480082265224 2313 62-63 0.900025436933781 2313 64-65 1.0146171888566187 2313 66-67 1.0252865662993358 2313 68-69 1.1009653130245454 2313 70-71 1.0412771392901305 2313 72-73 1.0391353887199486 2313 74-75 1.0679041440979944 2313 76-77 0.9900583096716531 2313 78-79 0.8964862298588585 2313 80-81 0.7772986930046386 2313 82-83 0.846505492180377 2313 84-85 0.8486447058670876 2313 86-87 0.9417203294501739 2313 88-89 0.9271563801587419 2313 90-91 0.7389036442633738 2313 92-93 0.9839413989892591 2313 94-95 1.140415820030693 2313 96-97 1.1835751703562352 2313 98-99 1.216746400012795 2313 100 1.2244409206403333 2314 1 -0.4071801972173219 2314 2 0.00715397812016505 2314 3 0.25284572920051573 2314 4 0.21241425266528324 2314 5 0.3671694384266999 2314 6 0.4025014099457138 2314 7 0.44383233731603866 2314 8 0.4370805444772117 2314 9 0.44114393687836895 2314 10-11 0.5499031974271631 2314 12-13 0.45114805425550486 2314 14-15 0.7430799221640569 2314 16-17 0.8078081128458976 2314 18-19 0.8388836194658538 2314 20-21 0.8631554854320882 2314 22-23 0.8871333411923956 2314 24-25 0.814302795594557 2314 26-27 0.7422848782594755 2314 28-29 0.836740235250339 2314 30-31 0.8637843014744035 2314 32-33 0.8625434402413674 2314 34-35 0.8529604938891922 2314 36-37 0.7519617863350234 2314 38-39 0.5284683759601236 2314 40-41 0.5133499654947045 2314 42-43 0.7096318322488528 2314 44-45 0.7799580772472439 2314 46-47 0.7559837065092339 2314 48-49 0.7783044715353782 2314 50-51 0.7441676601039617 2314 52-53 0.7821501548145449 2314 54-55 0.7609153237454294 2314 56-57 0.7470117924648605 2314 58-59 0.8300467821103084 2314 60-61 0.8528588541416013 2314 62-63 0.8418213403679786 2314 64-65 0.8755212809919399 2314 66-67 0.5488540018920673 2314 68-69 0.5233384508565635 2314 70-71 0.6833606046090424 2314 72-73 0.8544549994889223 2314 74-75 0.853456000788853 2314 76-77 0.7372696157255376 2314 78-79 0.6718543046429559 2314 80-81 0.7399601239323843 2314 82-83 0.6978078617725387 2314 84-85 0.7438289989583637 2314 86-87 0.7553862438480472 2314 88-89 0.7161176739598147 2314 90-91 0.8357129115520792 2314 92-93 0.8150517018272083 2314 94-95 0.7934790775055554 2314 96-97 0.8197231405087138 2314 98-99 0.8547337680324709 2314 100 0.8067751836328014 2315 1 0.39218083054085895 2315 2 0.4146836736920676 2315 3 0.46561457400905937 2315 4 0.3090952471786963 2315 5 0.3600283321812938 2315 6 0.37093662144297923 2315 7 0.3025248623516603 2315 8 0.2412316307770368 2315 9 0.4121702140481531 2315 10-11 0.5137395462475851 2315 12-13 0.3681989576834823 2315 14-15 0.6034123847706496 2315 16-17 0.7390160266215062 2315 18-19 0.7530338765699511 2315 20-21 0.46540949800210285 2315 22-23 0.8076494599665622 2315 24-25 0.896151369922876 2315 26-27 0.7999528657266595 2315 28-29 0.7288168854192918 2315 30-31 0.8006120251633106 2315 32-33 0.7510177051476035 2315 34-35 0.8068973463605289 2315 36-37 0.7833607891203016 2315 38-39 0.8377588301862744 2315 40-41 0.8653467457362538 2315 42-43 0.9578131901765232 2315 44-45 0.9546030243999013 2315 46-47 0.9448263010113678 2315 48-49 0.8055056495916944 2315 50-51 0.9130235602055379 2315 52-53 0.9206419119038003 2315 54-55 0.770003137923517 2315 56-57 0.7653904028054797 2315 58-59 0.8500219468434977 2315 60-61 0.9345740625956367 2315 62-63 1.0060738898776904 2315 64-65 1.0802121352409202 2315 66-67 1.0611745787900375 2315 68-69 1.089222207874002 2315 70-71 0.9892974109189723 2315 72-73 1.0780722354761068 2315 74-75 0.4187392747784173 2315 76-77 0.4635994757688451 2315 78-79 0.7188342049813272 2315 80-81 0.8053438417340004 2315 82-83 0.723729962680963 2315 84-85 0.7891793907853994 2315 86-87 0.8034855263586671 2315 88-89 0.8377044703428993 2315 90-91 0.8478985304344917 2315 92-93 0.8823285397192322 2315 94-95 0.8997363316324645 2315 96-97 1.0354876792696608 2315 98-99 0.9935528223518446 2315 100 0.904366690616957 2316 1 0.25378826072834215 2316 2 0.026831467588756652 2316 3 0.19943190102554098 2316 4 0.004856945823902947 2316 5 0.26895568198021635 2316 6 0.3635200735902657 2316 7 0.39604326839060633 2316 8 0.41206712194218653 2316 9 0.5570995756922343 2316 10-11 0.590209269469689 2316 12-13 0.36738708012471477 2316 14-15 0.4930810057402937 2316 16-17 0.7519832638082491 2316 18-19 0.6851109788309344 2316 20-21 0.6637099790121113 2316 22-23 0.7116403299404155 2316 24-25 0.3522525461276871 2316 26-27 0.6541440677402122 2316 28-29 0.8514571764548649 2316 30-31 0.48212745179596084 2316 32-33 0.6599974822675563 2316 34-35 0.7390048181062525 2316 36-37 0.7720619555216359 2316 38-39 0.7475324762933298 2316 40-41 0.7009868811948152 2316 42-43 0.7079960464425525 2316 44-45 0.55286876698176 2316 46-47 0.7090328262947665 2316 48-49 0.8013141278993601 2316 50-51 0.8269400302697534 2316 52-53 0.8471735462509429 2316 54-55 0.679624510368491 2316 56-57 0.8095439040341148 2316 58-59 0.6746390249367664 2316 60-61 0.7931362326604869 2316 62-63 0.8435503668469622 2316 64-65 0.8676663412547114 2316 66-67 0.8046983128087604 2316 68-69 0.8416982266370141 2316 70-71 0.8141090668337725 2316 72-73 0.8434838432259681 2316 74-75 0.8619483383113788 2316 76-77 0.683507045975631 2316 78-79 0.43094086687412414 2316 80-81 0.44886810855069825 2316 82-83 0.37955451017564457 2316 84-85 0.5459318592384363 2316 86-87 0.5454476295040926 2316 88-89 0.5295486120160149 2316 90-91 0.42119618761522304 2316 92-93 0.27998540077191336 2316 94-95 0.39063369129880243 2316 96-97 0.5586933755194003 2316 98-99 0.7258362239898766 2316 100 0.34752204811374554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 2.0 12 26.0 13 113.0 14 450.0 15 1306.0 16 3179.0 17 6281.0 18 11047.0 19 15907.0 20 22859.0 21 31842.0 22 39976.0 23 42357.0 24 42504.0 25 44889.0 26 53010.0 27 65734.0 28 80886.0 29 99740.0 30 125019.0 31 155099.0 32 197970.0 33 261290.0 34 369298.0 35 518452.0 36 811364.0 37 1277164.0 38 1216494.0 39 205290.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.179107741947604 20.573021481898724 13.847322876537929 23.40054789961575 2 15.301129079431727 20.365064279083494 41.15119326815799 23.18261337332679 3 18.706509001773632 26.141839101312215 29.62571577403954 25.525936122874608 4 12.489315718031136 16.78410747501326 38.81512322158262 31.911453585372985 5 12.86285228136225 37.966442902386646 34.78537700634729 14.385327809903819 6 30.27283147463532 37.50384895498789 18.165979746996236 14.057339823380557 7 27.43412936791599 32.091888770731266 22.634744575135237 17.83923728621751 8 25.787112391591055 36.182440203249385 19.960070875161566 18.070376529997986 9 26.20119082044904 16.185307131446475 21.30144476294489 36.3120572851596 10-11 25.20880050965168 26.536530154244726 27.78172738723334 20.472941948870258 12-13 25.4344757793027 24.03352622118909 28.37361263253894 22.15838536696927 14-15 22.587208162403805 25.67865561687667 26.181138643961667 25.552997576757857 16-17 21.631172823260663 28.357773730695946 27.264701960495337 22.746351485548058 18-19 21.325079185395325 27.759444129315064 29.444159141722075 21.47131754356754 20-21 22.46051388185623 26.966945738980186 29.111711036462573 21.46082934270101 22-23 22.439683914605222 26.95617709245861 28.757593297353022 21.846545695583146 24-25 22.012517980549905 26.860285655308747 29.396103542351458 21.731092821789886 26-27 21.931290121971408 27.123704957923607 28.79877766292513 22.146227257179856 28-29 21.460483487239088 27.50834621144812 29.029648087091147 22.00152221422165 30-31 22.33624764451369 27.0478782200419 28.993957322272635 21.621916813171772 32-33 22.28644567205903 27.17523169268297 28.64506731412842 21.893255321129583 34-35 22.248773515121893 27.251910473404294 29.01755731420213 21.48175869727168 36-37 22.098147139978387 27.017113691479693 28.825123808902024 22.059615359639896 38-39 22.208190007169563 27.168358180824757 28.94093609332662 21.682515718679056 40-41 22.214182560253747 26.629097427661026 29.256660340404046 21.900059671681184 42-43 22.025045305882472 26.820792930580456 28.82582276517736 22.328338998359705 44-45 21.950747238591955 27.34131088545354 28.72117922785594 21.986762648098566 46-47 22.102019119075827 27.09337700760518 28.34861748590584 22.455986387413148 48-49 22.406412876002836 27.04792085003207 28.391156731439764 22.154509542525332 50-51 22.232740777030894 27.17705131705564 28.190532107464495 22.39967579844897 52-53 21.940796518509543 27.448567834167715 28.058040984299094 22.552594663023648 54-55 21.91948706993173 27.37312407522518 28.125174097838705 22.582214757004383 56-57 22.432378279499485 27.160836932725147 27.73980125532189 22.66698353245348 58-59 21.799021111344285 27.5019143319771 28.158191551110733 22.54087300556788 60-61 22.08338476463239 27.578370552925286 27.982513095132504 22.35573158730982 62-63 22.183760897078976 27.265626994411196 27.716901799019762 22.833710309490073 64-65 21.861407221212477 27.34928193050554 27.82029276352653 22.969018084755447 66-67 22.05462909864612 27.23374847384708 27.755657880865403 22.955964546641404 68-69 21.798923769587255 27.098099813594306 27.982122398562687 23.12085401825575 70-71 21.885940798038398 26.936771403413424 27.99894603206703 23.17834176648115 72-73 22.0329836024597 27.234730080225354 27.869674871755983 22.862611445558958 74-75 22.12362665232606 27.374221028107755 27.73356695323308 22.76858536633311 76-77 22.017092697035938 27.177291757894118 27.55666924955531 23.248946295514635 78-79 21.857676149150144 27.273489807018287 27.606030532750403 23.262803511081167 80-81 21.634741238403436 27.61410936897977 27.757884331555644 22.993265061061155 82-83 22.143686912190045 27.089129688251578 28.258968419883118 22.50821497967526 84-85 22.323269675251886 27.31905352947181 27.47541122891236 22.882265566363944 86-87 21.5083988168292 27.74497434175196 27.65490582437227 23.09172101704657 88-89 21.913514677056988 26.977954391107733 27.7978567851848 23.310674146650477 90-91 21.95089348171478 27.05099513600628 27.78911658365748 23.20899479862146 92-93 22.04255298235785 27.225717139687234 27.670996596470516 23.060733281484403 94-95 21.343248120490742 27.21051591660601 27.96129309394844 23.484942868954807 96-97 21.5881455373422 27.09950776306831 27.749779164943895 23.562567534645595 98-99 21.269921409408127 27.399676961500717 27.649168396979356 23.681233232111794 100 21.455911463667064 27.119797943739528 27.48669271523016 23.937597877363253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1231.0 1 851.5 2 885.5 3 1331.5 4 1001.0 5 695.0 6 986.5 7 1870.0 8 3015.5 9 3538.5 10 3229.0 11 2892.0 12 3075.0 13 3544.0 14 4167.0 15 4761.5 16 5383.0 17 6192.0 18 6997.0 19 7705.0 20 8501.0 21 9428.5 22 10779.5 23 12540.5 24 14644.5 25 17559.5 26 21604.5 27 26280.0 28 32147.0 29 42014.5 30 54818.0 31 67842.0 32 84181.0 33 102363.5 34 119213.0 35 137355.5 36 154117.5 37 173645.0 38 184603.0 39 182481.0 40 178494.0 41 176023.0 42 180510.5 43 191016.0 44 206209.5 45 227826.0 46 243169.5 47 249244.0 48 253131.0 49 250171.0 50 242464.5 51 239101.0 52 231605.5 53 214426.5 54 196645.0 55 172080.5 56 149405.0 57 125190.5 58 101593.0 59 89644.5 60 72112.0 61 52236.5 62 39513.0 63 31114.0 64 22159.5 65 13086.0 66 8638.5 67 6235.5 68 4685.5 69 3493.0 70 2603.5 71 1986.0 72 1477.5 73 1148.5 74 873.5 75 553.5 76 342.5 77 197.5 78 114.0 79 73.0 80 46.5 81 34.5 82 23.0 83 15.5 84 10.0 85 5.5 86 5.0 87 4.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04509089148201479 2 0.0 3 0.0 4 0.034686650762623825 5 0.0 6 0.0 7 9.825252618649136E-4 8 0.0 9 0.011772758048417091 10-11 3.5090187923746916E-4 12-13 8.772546980936729E-6 14-15 6.14078288665571E-5 16-17 3.333567852755957E-4 18-19 0.0 20-21 1.7545093961873457E-5 22-23 0.0 24-25 6.579410235702547E-4 26-27 4.4739989602777315E-4 28-29 1.228156577331142E-4 30-31 0.002438768060700411 32-33 0.014606290723259653 34-35 0.024633311922470334 36-37 0.029914385204994245 38-39 0.021115520583114705 40-41 0.0013685173290261298 42-43 1.4036075169498766E-4 44-45 0.01139553852823681 46-47 0.018413576112986194 48-49 0.003342340399736894 50-51 0.011842938424264584 52-53 0.01158853456181742 54-55 0.010114746669020049 56-57 4.3862734904683644E-4 58-59 0.00847428038358488 60-61 0.0043599558495255545 62-63 0.0013772898760070664 64-65 0.03623939157824963 66-67 0.061328875943728677 68-69 0.0600217664435691 70-71 0.05317917979843845 72-73 0.04644186371707904 74-75 0.03552881527279375 76-77 0.032353153265694656 78-79 0.015764266924743302 80-81 0.0011755212954455217 82-83 0.0391694222698825 84-85 0.05343358366088561 86-87 0.059302417591132285 88-89 0.07093481488785439 90-91 0.04982806685172063 92-93 0.039967724045147736 94-95 0.013527267444604437 96-97 0.0036756971850124896 98-99 0.021176928411981263 100 0.031230267252134757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5699599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.38397444296432 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37988487673883 53.48932133819619 2 13.483956871513051 18.172052104401374 3 3.7441504880704293 7.568872223962507 4 1.3590170531314731 3.6630388150305553 5 0.6491750836327552 2.1871998622259405 6 0.3610731516523712 1.4598326413790377 7 0.2259575512872237 1.0658142502793158 8 0.14075930255058336 0.7587936996542394 9 0.10458107852669561 0.6342379850394452 >10 0.4989766722607366 5.970149986134008 >50 0.034911778732391485 1.6150060148773897 >100 0.016440058292006687 1.9759557979802862 >500 7.540772851843957E-4 0.3482702815686335 >1k 2.7146724482661277E-4 0.3194894065861802 >5k 3.016302720240938E-5 0.16993771413177516 >10k+ 6.032605440481876E-5 0.602027878553185 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16124 0.2828970950412476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13496 0.2367885881094442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8361 0.14669453061522397 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.5090187923746915E-5 2 0.0 0.0 0.0 0.0 3.5090187923746915E-5 3 0.0 0.0 0.0 0.0 3.5090187923746915E-5 4 0.0 0.0 0.0 0.0 3.5090187923746915E-5 5 0.0 0.0 0.0 0.0 3.5090187923746915E-5 6 0.0 0.0 0.0 0.0 3.5090187923746915E-5 7 0.0 0.0 0.0 0.0 3.5090187923746915E-5 8 0.0 0.0 0.0 1.7545093961873457E-5 3.5090187923746915E-5 9 0.0 0.0 0.0 1.7545093961873457E-5 3.5090187923746915E-5 10-11 0.0 0.0 0.0 3.5090187923746915E-5 3.5090187923746915E-5 12-13 0.0 0.0 0.0 6.14078288665571E-5 3.5090187923746915E-5 14-15 0.0 8.772546980936729E-6 0.0 7.018037584749383E-5 5.263528188562037E-5 16-17 0.0 1.7545093961873457E-5 0.0 1.0527056377124075E-4 7.018037584749383E-5 18-19 0.0 1.7545093961873457E-5 0.0 1.491332986759244E-4 8.772546980936729E-5 20-21 0.0 1.7545093961873457E-5 0.0 3.070391443327855E-4 8.772546980936729E-5 22-23 0.0 1.7545093961873457E-5 0.0 5.438979128180772E-4 1.0527056377124075E-4 24-25 0.0 1.7545093961873457E-5 0.0 8.246194162080526E-4 1.0527056377124075E-4 26-27 0.0 1.7545093961873457E-5 0.0 0.0014211526109117502 1.0527056377124075E-4 28-29 0.0 1.7545093961873457E-5 0.0 0.004105551987078389 1.0527056377124075E-4 30-31 0.0 1.7545093961873457E-5 0.0 0.014702788740049957 1.0527056377124075E-4 32-33 0.0 1.7545093961873457E-5 0.0 0.04107306496474576 1.0527056377124075E-4 34-35 0.0 3.5090187923746915E-5 0.0 0.08269002784230961 1.1404311075217748E-4 36-37 0.0 3.5090187923746915E-5 0.0 0.14861571840404914 1.228156577331142E-4 38-39 0.0 3.5090187923746915E-5 0.0 0.24404348446267887 1.228156577331142E-4 40-41 0.0 3.5090187923746915E-5 0.0 0.35052465971728886 1.228156577331142E-4 42-43 0.0 5.2635281885620375E-5 0.0 0.4522335694142693 1.228156577331142E-4 44-45 0.0 5.2635281885620375E-5 0.0 0.5695400676433553 1.228156577331142E-4 46-47 0.0 5.2635281885620375E-5 0.0 0.7109184347881315 1.3158820471405092E-4 48-49 0.0 6.14078288665571E-5 0.0 0.8649204970384758 1.7545093961873458E-4 50-51 0.0 7.018037584749383E-5 0.0 1.0227386172255277 1.7545093961873458E-4 52-53 0.0 7.018037584749383E-5 0.0 1.1758107894958925 1.7545093961873458E-4 54-55 0.0 7.018037584749383E-5 0.0 1.3445419581272295 1.7545093961873458E-4 56-57 0.0 7.018037584749383E-5 0.0 1.538608242439512 1.7545093961873458E-4 58-59 0.0 7.018037584749383E-5 0.0 1.7597466067349652 1.7545093961873458E-4 60-61 0.0 8.772546980936729E-5 0.0 1.986876620618398 1.7545093961873458E-4 62-63 0.0 1.0527056377124075E-4 0.0 2.214506669679744 1.7545093961873458E-4 64-65 0.0 1.0527056377124075E-4 0.0 2.443566643898983 1.7545093961873458E-4 66-67 0.0 1.0527056377124075E-4 0.0 2.7020672857862458 1.7545093961873458E-4 68-69 0.0 1.0527056377124075E-4 0.0 2.990920940227549 1.7545093961873458E-4 70-71 0.0 1.0527056377124075E-4 0.0 3.2968810612816797 1.7545093961873458E-4 72-73 0.0 1.0527056377124075E-4 0.0 3.5930597924520655 1.9299603358060804E-4 74-75 0.0 1.0527056377124075E-4 0.0 3.8920457386563507 1.9299603358060804E-4 76-77 0.0 1.0527056377124075E-4 0.0 4.224367363388196 1.9299603358060804E-4 78-79 0.0 1.0527056377124075E-4 0.0 4.58430496601603 1.9299603358060804E-4 80-81 0.0 1.0527056377124075E-4 0.0 4.964752783485294 1.9299603358060804E-4 82-83 0.0 1.0527056377124075E-4 0.0 5.344797063793435 1.9299603358060804E-4 84-85 0.0 1.0527056377124075E-4 0.0 5.724569395145167 1.9299603358060804E-4 86-87 0.0 1.0527056377124075E-4 0.0 6.128992583513332 1.9299603358060804E-4 88 0.0 1.0527056377124075E-4 0.0 6.45778764435884 1.9299603358060804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9245 0.0 50.300453 1 GTATCAA 16225 0.0 40.13725 1 TACCTGG 4525 0.0 38.120155 2 ACCTGGG 4975 0.0 34.29842 3 CAACGCA 19225 0.0 32.703773 5 GTACCTG 5410 0.0 32.505505 1 ATCAACG 19465 0.0 32.239433 3 TATCAAC 20780 0.0 31.145521 2 GTACTAG 3540 0.0 30.284063 1 AACGCAG 21520 0.0 29.827477 6 TCAACGC 21440 0.0 29.33539 4 GTACATG 21495 0.0 26.906065 1 CTGTGCG 4630 0.0 26.590382 9 CGCAGAG 23820 0.0 26.43427 8 ACGCAGA 24055 0.0 26.332533 7 CCTGGGG 4680 0.0 26.315763 4 GTGGTAT 3440 0.0 25.970345 1 TACATGG 21845 0.0 25.969429 2 ACATGGG 21390 0.0 25.646057 3 GGGCACC 4255 0.0 25.078531 94 >>END_MODULE