FastQCFastQC Report
Mon 6 Jun 2016
ERR1211213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7676849
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194540.25341126287621396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154020.20062919043998392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.12432184090113013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118750.047.5225521
GTATCAA212700.041.1363831
CAACGCA239150.034.556095
ATCAACG240000.034.4924663
GTACTAG53250.033.1455961
TATCAAC263900.032.726712
AACGCAG269150.031.3156936
TCAACGC274800.030.3468074
GCATCGT52200.029.4462998
ACGCAGA290500.028.301997
CTGTGCG66400.028.1043199
CGCAGAG295100.027.8117628
GTACATG289100.027.3999791
TAGGCAT68700.027.028165
TACCTGG46400.026.7788312
TAGTACT70850.026.6724154
TACATGG293950.026.4029772
CTAGTAC77700.025.7729843
CTAGGCA77200.025.6963394
ACATGGG292000.025.5326823
Produced by FastQC (version 0.11.5)