##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7676849 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.075366989763637 31.0 31.0 34.0 28.0 34.0 2 31.090120308475523 31.0 31.0 34.0 27.0 34.0 3 31.147032591106065 31.0 31.0 34.0 27.0 34.0 4 34.73033402115894 37.0 35.0 37.0 32.0 37.0 5 34.786881961596485 37.0 35.0 37.0 32.0 37.0 6 34.84961329837281 37.0 35.0 37.0 32.0 37.0 7 34.749151507343704 37.0 35.0 37.0 31.0 37.0 8 34.74682920036593 37.0 35.0 37.0 31.0 37.0 9 36.254847138454856 39.0 35.0 39.0 31.0 39.0 10-11 36.19516229901096 39.0 35.0 39.0 30.5 39.0 12-13 36.084334796737565 39.0 35.0 39.0 30.0 39.0 14-15 37.22013172331512 40.0 36.5 41.0 31.0 41.0 16-17 37.19049065573649 40.0 36.0 41.0 31.0 41.0 18-19 37.19620504454366 40.0 36.5 41.0 31.0 41.0 20-21 37.156530563516355 40.0 36.0 41.0 30.5 41.0 22-23 37.04704755818436 40.0 36.0 41.0 30.0 41.0 24-25 36.94749427792574 40.0 36.0 41.0 30.0 41.0 26-27 36.79721341399316 39.0 36.0 41.0 30.0 41.0 28-29 36.66803867055351 39.0 36.0 41.0 30.0 41.0 30-31 36.51522004666238 39.0 35.5 41.0 29.0 41.0 32-33 36.33997086565074 39.0 35.0 40.5 29.0 41.0 34-35 36.191552289227005 39.0 35.0 40.0 28.0 41.0 36-37 36.081962469237055 39.0 35.0 40.0 28.0 41.0 38-39 35.9392937128241 39.0 35.0 40.0 27.0 41.0 40-41 35.77574002041723 38.0 35.0 40.0 27.0 41.0 42-43 35.560268347078335 38.0 34.0 40.0 26.0 41.0 44-45 35.343665610721274 38.0 34.0 40.0 26.0 41.0 46-47 35.12443067461663 38.0 33.5 40.0 25.0 41.0 48-49 34.92584060204909 38.0 33.0 40.0 24.0 41.0 50-51 34.27425672955141 37.5 32.5 39.5 23.5 40.5 52-53 34.40799311019404 37.5 33.0 39.5 24.0 40.5 54-55 34.89723947937494 38.0 33.5 40.0 24.5 41.0 56-57 34.86152261168613 38.0 33.0 40.0 25.0 41.0 58-59 34.64948561577803 37.5 33.0 40.0 24.5 41.0 60-61 34.432386321523325 37.0 33.0 40.0 24.0 41.0 62-63 34.13740012341 36.5 33.0 40.0 23.5 41.0 64-65 33.89016854441191 36.0 32.0 39.5 23.0 41.0 66-67 33.71936252751617 36.0 32.0 39.0 23.0 41.0 68-69 33.42736381814987 35.0 32.0 39.0 23.0 41.0 70-71 33.12575354810288 35.0 32.0 38.5 23.0 40.5 72-73 32.79167767921448 35.0 31.5 37.5 22.0 40.0 74-75 32.4595104710279 35.0 31.0 37.0 21.5 39.5 76-77 32.13052972645417 35.0 31.0 36.5 21.0 39.0 78-79 31.794774392462323 35.0 31.0 36.0 20.0 39.0 80-81 31.495669837976493 35.0 30.0 36.0 20.0 37.5 82-83 31.207367957869174 34.0 30.0 35.0 20.0 37.0 84-85 30.956460326365672 34.0 30.0 35.0 19.0 37.0 86-87 30.73449484287108 34.0 30.0 35.0 19.0 36.0 88-89 30.51984329768633 34.0 29.0 35.0 18.0 36.0 90-91 30.291473298484835 34.0 29.0 35.0 18.0 36.0 92-93 30.09457845269589 34.0 29.0 35.0 18.0 35.0 94-95 29.919901641936683 34.0 29.0 35.0 17.0 35.0 96-97 29.739323907504236 34.0 29.0 35.0 17.0 35.0 98-99 29.498317082959428 34.0 27.5 35.0 15.0 35.0 100 28.34601787790798 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4324149144385778 1101 2 -0.33337890389836744 1101 3 -0.4155441688986947 1101 4 -0.3446450747333998 1101 5 -0.2638309762766582 1101 6 -0.32583584791640874 1101 7 -0.3205658857775333 1101 8 -0.26784934229318225 1101 9 -0.45042765947211905 1101 10-11 -0.4226384123073288 1101 12-13 -0.4307205498746072 1101 14-15 -0.5799577108930478 1101 16-17 -0.6061177126096027 1101 18-19 -0.618761826634362 1101 20-21 -0.5886037260588282 1101 22-23 -0.7297194255013224 1101 24-25 -0.7830391946050952 1101 26-27 -0.7959145422131755 1101 28-29 -0.8626372649282743 1101 30-31 -0.8287112876282379 1101 32-33 -0.7902015576209322 1101 34-35 -0.8023063612521355 1101 36-37 -0.9342063417685367 1101 38-39 -0.9648499078534272 1101 40-41 -0.9562036750333789 1101 42-43 -1.0233238486412048 1101 44-45 -0.9986883089172736 1101 46-47 -1.1669537158429009 1101 48-49 -1.0969025444471256 1101 50-51 -1.167053728674702 1101 52-53 -1.0126804910695668 1101 54-55 -0.8221361784251968 1101 56-57 -0.8413801287159117 1101 58-59 -0.8286726876731692 1101 60-61 -0.8068427396112767 1101 62-63 -0.9038493426913661 1101 64-65 -0.8560733467631252 1101 66-67 -0.8651799842777805 1101 68-69 -0.8882453974848019 1101 70-71 -0.9252457182479858 1101 72-73 -0.9587772521978835 1101 74-75 -1.0406315463147813 1101 76-77 -1.0800721100339459 1101 78-79 -1.0122752203103857 1101 80-81 -1.258774801655818 1101 82-83 -1.2546722679346125 1101 84-85 -1.2290347585877868 1101 86-87 -1.3287072302928955 1101 88-89 -1.4028787480541212 1101 90-91 -1.380149077705557 1101 92-93 -1.6320789449366124 1101 94-95 -1.6667752084529255 1101 96-97 -1.6445966985917408 1101 98-99 -1.7818350265881833 1101 100 -1.8020975883266814 1102 1 -0.5339975964085788 1102 2 -0.4863841618658853 1102 3 -0.5022600813759226 1102 4 -0.28150143463101074 1102 5 -0.358709000775022 1102 6 -0.3982297089036564 1102 7 -0.4290919211619979 1102 8 -0.4496097618419057 1102 9 -0.558589426015061 1102 10-11 -0.5523665605690411 1102 12-13 -0.6280386321150218 1102 14-15 -0.7877618253445817 1102 16-17 -0.8708499878611846 1102 18-19 -0.8715476869869363 1102 20-21 -0.7502064933729002 1102 22-23 -0.8337710863730123 1102 24-25 -0.8073593535486552 1102 26-27 -0.9163997372716395 1102 28-29 -0.8649719813119461 1102 30-31 -0.8999330400984178 1102 32-33 -0.9608904436757726 1102 34-35 -1.0598865426340893 1102 36-37 -0.9990997025496924 1102 38-39 -1.1721751558172429 1102 40-41 -1.2359336644410988 1102 42-43 -1.1167818524167714 1102 44-45 -1.1926736535635882 1102 46-47 -1.2640982985109233 1102 48-49 -1.1222601596734734 1102 50-51 -1.1606781391867287 1102 52-53 -1.105547758725045 1102 54-55 -0.9988207438117271 1102 56-57 -0.9464133584521335 1102 58-59 -0.9805913219119091 1102 60-61 -1.003986776676534 1102 62-63 -1.0582708716792837 1102 64-65 -1.097011516824935 1102 66-67 -1.0134503898106217 1102 68-69 -1.1102352364654209 1102 70-71 -1.1409763981065204 1102 72-73 -1.1598600251086992 1102 74-75 -1.1887964546027519 1102 76-77 -1.2047357375036256 1102 78-79 -1.2510066273162685 1102 80-81 -1.3499689436769309 1102 82-83 -1.2775310119688505 1102 84-85 -1.2856746030855248 1102 86-87 -1.342904361922237 1102 88-89 -1.4999224784737777 1102 90-91 -1.4896052288753587 1102 92-93 -1.447479990142984 1102 94-95 -1.4670056740096555 1102 96-97 -1.5671636589022384 1102 98-99 -1.6669561948505205 1102 100 -1.7878162779851756 1103 1 -0.5552739776062694 1103 2 -0.4360372416297267 1103 3 -0.5013777365428531 1103 4 -0.44504312747262986 1103 5 -0.37438408514272936 1103 6 -0.4232800806875474 1103 7 -0.3810252201944593 1103 8 -0.4293625491538009 1103 9 -0.5975378928156232 1103 10-11 -0.5878941393901016 1103 12-13 -0.6681022229090132 1103 14-15 -0.770725125199192 1103 16-17 -0.8792597906734514 1103 18-19 -0.7725028683176305 1103 20-21 -0.817448779193505 1103 22-23 -0.9527436715342006 1103 24-25 -0.9832426315313683 1103 26-27 -0.9218661949941023 1103 28-29 -0.9905154037535482 1103 30-31 -0.9110382121220155 1103 32-33 -0.9595091077421429 1103 34-35 -0.9261321758393564 1103 36-37 -0.9574428959609946 1103 38-39 -0.9917660817155678 1103 40-41 -1.025885145132051 1103 42-43 -1.1739080845097547 1103 44-45 -1.2455397728963078 1103 46-47 -1.1769512699887485 1103 48-49 -1.2366847381276997 1103 50-51 -1.274873608055998 1103 52-53 -1.1778047785516534 1103 54-55 -1.0138469130685621 1103 56-57 -0.9372999628388783 1103 58-59 -0.9387886878195246 1103 60-61 -0.9688526352965923 1103 62-63 -1.0214703059669645 1103 64-65 -0.9979452296147286 1103 66-67 -1.0186443637701004 1103 68-69 -0.9918682938873431 1103 70-71 -0.982912618974872 1103 72-73 -1.0913344653876536 1103 74-75 -1.0217466578342034 1103 76-77 -1.0427690752201215 1103 78-79 -1.075846753775732 1103 80-81 -1.1092140780639 1103 82-83 -1.044768237638099 1103 84-85 -1.1752691957130494 1103 86-87 -1.2147556120947378 1103 88-89 -1.2716150057870053 1103 90-91 -1.3910928340864537 1103 92-93 -1.439722477231161 1103 94-95 -1.5176548513782677 1103 96-97 -1.533495152040068 1103 98-99 -1.485366554299766 1103 100 -1.418522000156397 1104 1 -0.5611012197645984 1104 2 -0.6228115317033698 1104 3 -0.6053828783546571 1104 4 -0.5094190112140069 1104 5 -0.4662378959788924 1104 6 -0.5136075140195118 1104 7 -0.5392945218047771 1104 8 -0.5330128090993469 1104 9 -0.6069399132571078 1104 10-11 -0.6373501377730122 1104 12-13 -0.6378334257947174 1104 14-15 -0.8377638576758457 1104 16-17 -0.8956835873215638 1104 18-19 -0.9842543789748106 1104 20-21 -0.9100723740913992 1104 22-23 -0.871310178877458 1104 24-25 -0.8978377084308775 1104 26-27 -0.9694773136140284 1104 28-29 -0.9986253759339903 1104 30-31 -1.0032569031933747 1104 32-33 -1.0855505041215778 1104 34-35 -1.24659330181953 1104 36-37 -1.1708879857620076 1104 38-39 -1.1906689956257566 1104 40-41 -1.1809047057965003 1104 42-43 -1.1518522548222094 1104 44-45 -1.2833968501875574 1104 46-47 -1.3423919920685492 1104 48-49 -1.2425937139405576 1104 50-51 -1.2656313507085883 1104 52-53 -1.1160213935761405 1104 54-55 -0.9711049097760949 1104 56-57 -1.0729736131855674 1104 58-59 -1.1089480076124119 1104 60-61 -1.0355456711555675 1104 62-63 -1.0193503204802639 1104 64-65 -1.0594106544116926 1104 66-67 -1.1417998453939404 1104 68-69 -1.2015030715392783 1104 70-71 -1.1182216902420379 1104 72-73 -1.0672117439617246 1104 74-75 -1.0756072991223604 1104 76-77 -1.070283080766682 1104 78-79 -1.2004452228929416 1104 80-81 -1.221565930544834 1104 82-83 -1.2969786601788087 1104 84-85 -1.2931683360219672 1104 86-87 -1.1968522074212338 1104 88-89 -1.2639670064365376 1104 90-91 -1.39955588119901 1104 92-93 -1.3809738381537287 1104 94-95 -1.4444522449119006 1104 96-97 -1.5910069145790473 1104 98-99 -1.578759812797113 1104 100 -1.5028583626341323 1105 1 -0.6048301660430369 1105 2 -0.5774598227736227 1105 3 -0.5582244612862439 1105 4 -0.3299584470993153 1105 5 -0.31410046380991474 1105 6 -0.36995574447831814 1105 7 -0.32702814666801316 1105 8 -0.4657887402251646 1105 9 -0.6196190172056859 1105 10-11 -0.7498649502727659 1105 12-13 -0.6736812345643415 1105 14-15 -0.8628356515935636 1105 16-17 -0.8100992758144656 1105 18-19 -0.8622359961813117 1105 20-21 -0.83231408242019 1105 22-23 -0.8102710446099692 1105 24-25 -0.8549091079483588 1105 26-27 -0.8090521686317587 1105 28-29 -0.861034301326491 1105 30-31 -0.882346031769174 1105 32-33 -0.957889517034225 1105 34-35 -0.9437916207245038 1105 36-37 -1.0161508766701601 1105 38-39 -0.9435523576334788 1105 40-41 -0.9881480976433323 1105 42-43 -1.0313898217445967 1105 44-45 -1.0209281110244177 1105 46-47 -1.0998820879804967 1105 48-49 -0.996718722981889 1105 50-51 -1.0560830445364502 1105 52-53 -0.9550845958541601 1105 54-55 -0.8431626066442419 1105 56-57 -0.7593632904550986 1105 58-59 -0.6653219316632573 1105 60-61 -0.6245851230543948 1105 62-63 -0.7788226734195334 1105 64-65 -0.7970643856382509 1105 66-67 -0.734800932513572 1105 68-69 -0.8409364798542356 1105 70-71 -0.6971274970778083 1105 72-73 -0.7583118531295057 1105 74-75 -0.7495996083177516 1105 76-77 -0.796303384462064 1105 78-79 -0.8655517415443654 1105 80-81 -0.9160089055845013 1105 82-83 -1.0319089246272846 1105 84-85 -1.0487047533300426 1105 86-87 -1.109810276366666 1105 88-89 -1.1008207037408368 1105 90-91 -1.0678185913403873 1105 92-93 -0.9839907480638637 1105 94-95 -1.1538868071594237 1105 96-97 -1.1990883529499925 1105 98-99 -1.227243495027114 1105 100 -1.2836214911060502 1106 1 -0.6234727412165526 1106 2 -0.6732448819515042 1106 3 -0.6736800531072582 1106 4 -0.5775608411191939 1106 5 -0.5142811012422683 1106 6 -0.575751343119471 1106 7 -0.5211418427487189 1106 8 -0.543409490585951 1106 9 -0.6516516825242604 1106 10-11 -0.6377141361554308 1106 12-13 -0.862380892451057 1106 14-15 -0.9634345074415123 1106 16-17 -0.9891531719487219 1106 18-19 -1.0247591051528886 1106 20-21 -0.8989598925821056 1106 22-23 -0.8954559205455794 1106 24-25 -0.8852113732371549 1106 26-27 -0.9876445395161966 1106 28-29 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1107 50-51 -1.1715733050256318 1107 52-53 -0.9830292319007938 1107 54-55 -0.8899821646169883 1107 56-57 -0.8801217803584862 1107 58-59 -0.9770966267958343 1107 60-61 -0.9832741487290804 1107 62-63 -0.9747425259101803 1107 64-65 -0.87636646789732 1107 66-67 -0.8989294784149635 1107 68-69 -0.8248297219717173 1107 70-71 -0.8899280236347167 1107 72-73 -0.870019686278674 1107 74-75 -0.8587070257611806 1107 76-77 -0.8278242287506643 1107 78-79 -0.7973496109090554 1107 80-81 -0.8908542106030417 1107 82-83 -0.9780638937895603 1107 84-85 -0.9860003316769053 1107 86-87 -1.0443485396220957 1107 88-89 -1.0233360731556687 1107 90-91 -1.0184202661179533 1107 92-93 -1.031473496449781 1107 94-95 -1.0371973171456261 1107 96-97 -1.0556389383998024 1107 98-99 -1.0976671014134425 1107 100 -1.1173351212548468 1108 1 -0.689126752234678 1108 2 -0.6673977995095228 1108 3 -0.7683211518479602 1108 4 -0.6008559386713301 1108 5 -0.5549596818327203 1108 6 -0.4987047782412404 1108 7 -0.5913756893302065 1108 8 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38-39 -0.0337514927237379 2116 40-41 -0.17999489219424447 2116 42-43 0.01887996793053759 2116 44-45 0.09751492263043815 2116 46-47 -0.1896702715351708 2116 48-49 -0.17559788180362546 2116 50-51 0.047988937235203366 2116 52-53 0.11030713261306602 2116 54-55 0.1463598031677833 2116 56-57 0.1973938053181854 2116 58-59 0.19366117364833002 2116 60-61 0.18895007760624338 2116 62-63 0.003042268235937229 2116 64-65 -0.2034969848195871 2116 66-67 0.027258319692698763 2116 68-69 -0.23936131124325755 2116 70-71 -0.03930363794938074 2116 72-73 0.03746321689901322 2116 74-75 0.1467495646887116 2116 76-77 0.12552683237055362 2116 78-79 -0.32423640520037367 2116 80-81 0.12791975219584018 2116 82-83 -0.14171970421910274 2116 84-85 0.27268819557253465 2116 86-87 0.44208535217842027 2116 88-89 0.44909316813813405 2116 90-91 0.520688503372714 2116 92-93 0.58308951341591 2116 94-95 0.5180300814357359 2116 96-97 0.6079029502843483 2116 98-99 0.6287538784669451 2116 100 0.28171387241746615 2201 1 1.0327526453702518 2201 2 1.0312594428534716 2201 3 1.0638078479637265 2201 4 0.8742774457310958 2201 5 0.9159249614454694 2201 6 0.9687415583286807 2201 7 1.0715177908113844 2201 8 1.0349678660031714 2201 9 1.2271291489359086 2201 10-11 1.2552969937163283 2201 12-13 1.2594450238996444 2201 14-15 1.4106522029328588 2201 16-17 1.4600794784181517 2201 18-19 1.434359906964275 2201 20-21 1.4471095543511154 2201 22-23 1.4213591632944471 2201 24-25 1.4664745657475748 2201 26-27 1.4659793549579874 2201 28-29 1.4495740954397363 2201 30-31 1.4315918531838037 2201 32-33 1.4282491102183812 2201 34-35 1.4255514903817996 2201 36-37 1.3859622407699206 2201 38-39 1.2806799505344912 2201 40-41 1.3599072981152318 2201 42-43 1.3856899126497453 2201 44-45 1.3447977270181681 2201 46-47 1.3761886370690846 2201 48-49 1.3407578109039875 2201 50-51 1.027044506329588 2201 52-53 0.9594111359129016 2201 54-55 0.9155229363058837 2201 56-57 0.9647575104654962 2201 58-59 1.1603705002907745 2201 60-61 1.2127695343117963 2201 62-63 1.1959872082264908 2201 64-65 0.6329989801333724 2201 66-67 0.6708972982180725 2201 68-69 0.9908371546228025 2201 70-71 0.9947537333771166 2201 72-73 1.0456384689390958 2201 74-75 1.0490629358869086 2201 76-77 1.0348495463751917 2201 78-79 1.0200799935985678 2201 80-81 0.9629171163198116 2201 82-83 0.9825558095276143 2201 84-85 0.9768474379727117 2201 86-87 0.9274211720032746 2201 88-89 0.9217853618927521 2201 90-91 0.9220787318244454 2201 92-93 0.9299340726078391 2201 94-95 0.9496957113093991 2201 96-97 0.6046977639268363 2201 98-99 0.5845748004568705 2201 100 0.7535741406952141 2202 1 0.9846062014693473 2202 2 0.9884285048086703 2202 3 1.0248641280167483 2202 4 0.9068333354448654 2202 5 0.8649366805758731 2202 6 -0.04265628018092116 2202 7 0.5037073368142799 2202 8 0.7092643706192874 2202 9 1.0769509476451802 2202 10-11 1.1444273864517527 2202 12-13 1.209329526895118 2202 14-15 1.399399427208202 2202 16-17 1.339783535133769 2202 18-19 1.4043807715114482 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0.9035211596634376 2202 88-89 0.8679190522621276 2202 90-91 0.8686146494122333 2202 92-93 0.9170462893775024 2202 94-95 0.909183666283937 2202 96-97 0.9085805224525778 2202 98-99 0.8962288700993426 2202 100 0.9411789205794392 2203 1 1.0471920780994566 2203 2 1.0950590637158832 2203 3 1.0975793924034853 2203 4 0.9614627272942471 2203 5 0.985468602007856 2203 6 0.9887435274459904 2203 7 -0.08266351704003227 2203 8 0.7178181193070117 2203 9 1.1063654944411923 2203 10-11 1.1573009556023592 2203 12-13 1.2657653541833156 2203 14-15 1.4957788356020032 2203 16-17 1.5566514679444268 2203 18-19 1.5136913943876351 2203 20-21 1.5082401549566455 2203 22-23 1.5093176671723043 2203 24-25 1.5751052949232616 2203 26-27 1.573084403241232 2203 28-29 1.5552729024461627 2203 30-31 1.5608642945894928 2203 32-33 1.5762320575524669 2203 34-35 1.5870311005295719 2203 36-37 1.5473914266636655 2203 38-39 1.615679649391545 2203 40-41 1.5133915219378622 2203 42-43 1.4697717718265082 2203 44-45 1.5070829564107058 2203 46-47 1.5860084133322587 2203 48-49 1.5135414469891018 2203 50-51 1.563302542652103 2203 52-53 1.3650120508374073 2203 54-55 0.8795350485774733 2203 56-57 0.9969815102102828 2203 58-59 1.3697209198420168 2203 60-61 1.4242889943336223 2203 62-63 1.4581514991641242 2203 64-65 1.455089561179733 2203 66-67 1.401780304323161 2203 68-69 1.3993474403730772 2203 70-71 1.346468639292631 2203 72-73 1.344749464726668 2203 74-75 1.339468246783305 2203 76-77 1.3076009917925475 2203 78-79 1.2932072351539823 2203 80-81 1.2386897679755258 2203 82-83 1.3175703042661375 2203 84-85 1.2807140170741604 2203 86-87 1.1881647970547355 2203 88-89 1.2219375284580742 2203 90-91 1.2245172608498152 2203 92-93 1.2189283019390516 2203 94-95 1.126428555168573 2203 96-97 1.2008465444904388 2203 98-99 1.1430310317368502 2203 100 1.0942776381060746 2204 1 0.9973939630863597 2204 2 1.0333018873248427 2204 3 1.0819475510106606 2204 4 0.9384896880050562 2204 5 0.9251152146749462 2204 6 0.9377568673952297 2204 7 0.973069416794587 2204 8 0.9413728218231441 2204 9 1.1702841079370856 2204 10-11 1.1377532134153512 2204 12-13 1.2293254548395467 2204 14-15 1.3538555300863564 2204 16-17 1.4057997613960467 2204 18-19 1.408726127874921 2204 20-21 1.4466725950522843 2204 22-23 1.462598400446936 2204 24-25 1.4648240872666207 2204 26-27 1.4635414866643046 2204 28-29 1.4811168389444092 2204 30-31 1.4899409127365146 2204 32-33 1.4956339409035664 2204 34-35 1.4834046000238388 2204 36-37 1.500112988961746 2204 38-39 1.5387396587156132 2204 40-41 1.5464958205477046 2204 42-43 0.36605128097744455 2204 44-45 1.2587956390409687 2204 46-47 1.413818990415244 2204 48-49 1.4661540922685958 2204 50-51 1.4691809263726228 2204 52-53 0.7927563780192273 2204 54-55 0.9284969289841385 2204 56-57 1.2639808122689402 2204 58-59 1.3295475114072843 2204 60-61 1.389910748292941 2204 62-63 1.3889476157748604 2204 64-65 1.3734463046290415 2204 66-67 1.3158716991971104 2204 68-69 1.3201797626911116 2204 70-71 1.2156601860233565 2204 72-73 1.204152384084452 2204 74-75 1.239637688402965 2204 76-77 1.2189813207519435 2204 78-79 1.2153471069617972 2204 80-81 1.12957652153953 2204 82-83 1.1792415329973274 2204 84-85 1.2013156234297924 2204 86-87 1.194118018626579 2204 88-89 1.1779109248969384 2204 90-91 1.222746439160197 2204 92-93 1.2513664891454575 2204 94-95 1.2473745655886184 2204 96-97 1.2717162857341364 2204 98-99 1.2343833748840751 2204 100 0.9380906334467696 2205 1 0.9465277134253647 2205 2 0.9786593793775822 2205 3 1.0250843492701698 2205 4 0.8351209151680692 2205 5 0.8226784000562333 2205 6 0.897121179224456 2205 7 0.9405695784843786 2205 8 0.8986851407373706 2205 9 1.1253067363554692 2205 10-11 1.1214347595284622 2205 12-13 1.1442812714056032 2205 14-15 1.4022135091107018 2205 16-17 1.4316750466630381 2205 18-19 1.4587326905782163 2205 20-21 1.4221645804018337 2205 22-23 1.4320248174609418 2205 24-25 1.419432151212085 2205 26-27 1.4612508042899108 2205 28-29 1.4718781424195697 2205 30-31 1.486511078225334 2205 32-33 1.5525597940414073 2205 34-35 1.461364284101009 2205 36-37 1.4720357061328784 2205 38-39 1.4639295694900198 2205 40-41 1.4414632325816683 2205 42-43 1.5299566030444893 2205 44-45 1.5667568095772282 2205 46-47 1.4693315389017982 2205 48-49 1.5036372099521316 2205 50-51 1.4694300917000263 2205 52-53 1.4406740988399278 2205 54-55 1.4403316026669586 2205 56-57 1.390423438480724 2205 58-59 1.3650290317587306 2205 60-61 1.371266312025078 2205 62-63 1.3029020969734617 2205 64-65 1.323320091480042 2205 66-67 1.333296817801326 2205 68-69 1.2868377515527243 2205 70-71 1.2720869204384968 2205 72-73 1.296683959790876 2205 74-75 1.2844333967482768 2205 76-77 1.3158575147427314 2205 78-79 1.346411955166328 2205 80-81 1.3151046194208185 2205 82-83 1.362119331385646 2205 84-85 1.333440273486886 2205 86-87 1.3137054460631887 2205 88-89 1.2869332152613708 2205 90-91 1.3435296509227364 2205 92-93 1.3847379577181265 2205 94-95 1.385805185450213 2205 96-97 1.391632405418278 2205 98-99 1.3645599890509281 2205 100 1.5468778621815744 2206 1 0.9946738511113438 2206 2 1.042443194910124 2206 3 1.067962266397135 2206 4 0.9318190022636088 2206 5 0.7913274472524989 2206 6 0.9209730460964423 2206 7 0.9751919292312152 2206 8 0.9755274188664842 2206 9 1.1578176088686334 2206 10-11 1.167965913727727 2206 12-13 1.2667672754182036 2206 14-15 1.5016115188639603 2206 16-17 1.5156807608931402 2206 18-19 1.4693920440197275 2206 20-21 1.536794449576206 2206 22-23 1.539908703408706 2206 24-25 1.5444092140568486 2206 26-27 1.5881098531822815 2206 28-29 1.5759905099647895 2206 30-31 1.5342879763005683 2206 32-33 1.5769416114029227 2206 34-35 1.6115477022608218 2206 36-37 1.6080366305086145 2206 38-39 1.6089348737532418 2206 40-41 1.6213766956040772 2206 42-43 1.695588545429409 2206 44-45 1.6755120594547677 2206 46-47 1.7073514744496308 2206 48-49 1.677837615694159 2206 50-51 1.6589084255279118 2206 52-53 1.5498757469748838 2206 54-55 1.5796158878264421 2206 56-57 1.5076303246912346 2206 58-59 1.4760670463019565 2206 60-61 1.4610651172851234 2206 62-63 1.4170681631301036 2206 64-65 1.460545581817783 2206 66-67 1.4693029696368356 2206 68-69 1.404878598548116 2206 70-71 1.392040964097113 2206 72-73 1.4595417428873745 2206 74-75 1.403502089671285 2206 76-77 1.3670486142391738 2206 78-79 1.3589621344239653 2206 80-81 1.3771012669838534 2206 82-83 1.3881835798687412 2206 84-85 1.3710886800646591 2206 86-87 1.3216340951511327 2206 88-89 1.3566773737292124 2206 90-91 1.3620400256744993 2206 92-93 1.4491940245779276 2206 94-95 1.460900339292074 2206 96-97 1.4590682181768884 2206 98-99 1.4903372955650163 2206 100 1.532729943352603 2207 1 1.061829792213146 2207 2 1.113400288784323 2207 3 1.0634447156870657 2207 4 0.927848392679337 2207 5 0.9451935041818302 2207 6 0.972079369481051 2207 7 1.0650564734162273 2207 8 1.016302623269958 2207 9 1.1747792850414598 2207 10-11 1.2481025471867895 2207 12-13 1.3289069479775435 2207 14-15 1.5754999587104024 2207 16-17 1.4949977744682812 2207 18-19 1.4884164113213743 2207 20-21 1.5167670986800559 2207 22-23 1.587453063939499 2207 24-25 1.650475315083611 2207 26-27 1.6438261089128758 2207 28-29 1.6243014050341316 2207 30-31 1.570935090685957 2207 32-33 1.6415444389562666 2207 34-35 1.6658344524753375 2207 36-37 1.7050038818873432 2207 38-39 1.7032794837104817 2207 40-41 1.729938438129608 2207 42-43 1.8003875006390544 2207 44-45 1.8053041267574272 2207 46-47 1.7761658824045057 2207 48-49 1.8420173599830605 2207 50-51 1.82978812909316 2207 52-53 1.6760196201776552 2207 54-55 1.5903557329833333 2207 56-57 1.6418546846627322 2207 58-59 1.5713803459684854 2207 60-61 1.5442731521491098 2207 62-63 1.5934315450039733 2207 64-65 1.5744116085579023 2207 66-67 1.5659352134521143 2207 68-69 1.619460516100105 2207 70-71 1.5635180697906534 2207 72-73 1.5941778810579947 2207 74-75 1.5424350000802463 2207 76-77 1.4986000902612275 2207 78-79 1.4745438153878716 2207 80-81 1.5271992468648072 2207 82-83 1.5836865644430596 2207 84-85 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48-49 1.7039350384001821 2310 50-51 1.6816252098323474 2310 52-53 1.5920389906346841 2310 54-55 1.491253404318293 2310 56-57 1.553364648742928 2310 58-59 1.576828364993311 2310 60-61 1.5403070477453937 2310 62-63 1.4900241980847682 2310 64-65 1.5274457265180672 2310 66-67 1.4634585506114348 2310 68-69 1.450378306764975 2310 70-71 1.4507363787656047 2310 72-73 1.473453596099283 2310 74-75 1.4236311446222842 2310 76-77 1.4446424837242589 2310 78-79 1.4848660134743135 2310 80-81 1.4589220199882895 2310 82-83 1.460536728621637 2310 84-85 1.4790375515215004 2310 86-87 1.4666249914096845 2310 88-89 1.5251988223017605 2310 90-91 1.5904603953220189 2310 92-93 1.5713694739719735 2310 94-95 1.686625233390437 2310 96-97 1.6277620266071118 2310 98-99 1.702678104195627 2310 100 1.8208400091957984 2311 1 0.8135336385492202 2311 2 0.7943594799631235 2311 3 0.8332535401310928 2311 4 0.7367915526865687 2311 5 0.7208147047495714 2311 6 0.7234919237597595 2311 7 0.7560510810793915 2311 8 0.6790864632662803 2311 9 0.959662068345942 2311 10-11 0.9882094779932373 2311 12-13 1.0362198524394515 2311 14-15 1.199851727783006 2311 16-17 1.197099946295559 2311 18-19 1.1242429268773009 2311 20-21 1.1866025213683713 2311 22-23 1.2399400383618229 2311 24-25 1.2499384348855926 2311 26-27 1.1940871451780026 2311 28-29 1.2923009932899134 2311 30-31 1.3516327787607025 2311 32-33 1.3565371331199785 2311 34-35 1.3876176853495679 2311 36-37 1.4121626330492276 2311 38-39 1.4059653199409183 2311 40-41 1.45462666303208 2311 42-43 1.4932019891896147 2311 44-45 1.5132115370884733 2311 46-47 1.504152131413143 2311 48-49 1.5295130098930585 2311 50-51 1.544999948189286 2311 52-53 1.3774915594543344 2311 54-55 1.2745144491130063 2311 56-57 1.29619117074067 2311 58-59 1.2022231823676037 2311 60-61 1.2152497066494305 2311 62-63 1.2448077900170986 2311 64-65 1.2187480190156492 2311 66-67 1.2778377811588015 2311 68-69 1.2467090592925842 2311 70-71 1.2449756597505157 2311 72-73 1.2566305354886964 2311 74-75 1.2974478470245216 2311 76-77 1.259939762109731 2311 78-79 1.2892218986969404 2311 80-81 1.2473426597829835 2311 82-83 1.330019197440837 2311 84-85 1.3622143357020207 2311 86-87 1.3262664789840635 2311 88-89 1.373924208128468 2311 90-91 1.4313301632734117 2311 92-93 1.4547879578261806 2311 94-95 1.5242880995130932 2311 96-97 1.5139734116699657 2311 98-99 1.549662625175273 2311 100 1.7234762410283615 2312 1 0.6928347399873367 2312 2 0.7119714685463912 2312 3 0.6976349817141454 2312 4 0.4114705942038128 2312 5 0.5335983151472306 2312 6 0.6474225355111258 2312 7 0.5013393300822457 2312 8 0.6424193500794075 2312 9 0.8533101067819118 2312 10-11 0.842948942985764 2312 12-13 0.9417529546677557 2312 14-15 1.184708102391518 2312 16-17 1.1982337428278313 2312 18-19 1.2153422386527666 2312 20-21 1.2147395542887551 2312 22-23 1.2789185768059852 2312 24-25 1.2382285727591693 2312 26-27 1.259712186844645 2312 28-29 1.265304372722106 2312 30-31 1.3184104742718148 2312 32-33 1.3542415197367674 2312 34-35 1.332747571569783 2312 36-37 1.3907676866385827 2312 38-39 1.4195813200118934 2312 40-41 1.4447058115031766 2312 42-43 1.460326204971814 2312 44-45 1.5096615552778516 2312 46-47 1.596562278055437 2312 48-49 1.6062174596938021 2312 50-51 1.607346428269743 2312 52-53 1.394170398340627 2312 54-55 1.3970883130943648 2312 56-57 1.3683188901113255 2312 58-59 1.3458411763362363 2312 60-61 1.3297696387686955 2312 62-63 1.339962478890449 2312 64-65 1.365163920290378 2312 66-67 1.311589522864061 2312 68-69 1.370322395113618 2312 70-71 1.3097827737617962 2312 72-73 1.3122733783512999 2312 74-75 1.347196928216725 2312 76-77 1.3270185556144227 2312 78-79 1.369890915358905 2312 80-81 1.3628815172029825 2312 82-83 1.3901899178894332 2312 84-85 1.358768306795099 2312 86-87 1.374727159359093 2312 88-89 1.3695527206539957 2312 90-91 1.40993157530173 2312 92-93 1.5005341881018985 2312 94-95 1.5958444242748016 2312 96-97 1.637682527536949 2312 98-99 1.247587924264053 2312 100 1.4397095485860518 2313 1 0.6852168608814182 2313 2 0.7231308829503078 2313 3 0.7500315950210883 2313 4 0.6598130939526499 2313 5 0.4482745978219569 2313 6 0.6202839320410689 2313 7 0.6905503058405813 2313 8 0.7073433931633417 2313 9 0.8303601098813189 2313 10-11 0.8568369923027177 2313 12-13 0.9570047443097423 2313 14-15 1.1112120322294388 2313 16-17 1.1061391189144913 2313 18-19 1.119548672172563 2313 20-21 1.121230060475817 2313 22-23 1.1388606071545482 2313 24-25 1.1289522742667657 2313 26-27 1.1557638807255515 2313 28-29 1.282691890864342 2313 30-31 1.2873272412239913 2313 32-33 1.2862987369797523 2313 34-35 1.340184017636851 2313 36-37 1.40500141634012 2313 38-39 1.3895907037423427 2313 40-41 1.4657002304279558 2313 42-43 1.4982235225929301 2313 44-45 1.5412144451034493 2313 46-47 1.5621613084745434 2313 48-49 1.5626924688317203 2313 50-51 1.5309457622172573 2313 52-53 1.484933259288276 2313 54-55 1.3118323980310223 2313 56-57 1.2848753816648966 2313 58-59 1.2324981101435313 2313 60-61 1.2455273803463243 2313 62-63 1.259252949167113 2313 64-65 1.266873683003837 2313 66-67 1.2530293590442199 2313 68-69 1.2894886752446837 2313 70-71 1.3120565008128082 2313 72-73 1.340240272379269 2313 74-75 1.255595003590095 2313 76-77 1.0899594039122107 2313 78-79 1.245355944397172 2313 80-81 1.280730911535553 2313 82-83 1.2869276714799476 2313 84-85 1.3164340450638292 2313 86-87 1.3533761159267392 2313 88-89 1.3556436808511592 2313 90-91 1.4177822307734722 2313 92-93 1.4234994041735156 2313 94-95 1.500386363564843 2313 96-97 1.5770764112257183 2313 98-99 1.605091253457715 2313 100 1.7016379800067973 2314 1 0.5887512591278323 2314 2 0.5744708689352116 2314 3 0.6193037189524802 2314 4 0.4871510149309799 2314 5 0.4746454709757657 2314 6 0.557749416654417 2314 7 0.5679578641580889 2314 8 0.5418119416050047 2314 9 0.7038866123390335 2314 10-11 0.7147626085483125 2314 12-13 0.8264169596224491 2314 14-15 0.9432372856383466 2314 16-17 0.9913002105899906 2314 18-19 0.8656536399619768 2314 20-21 0.909477562005641 2314 22-23 0.9380461635542972 2314 24-25 0.9125454930093184 2314 26-27 1.0123952910612601 2314 28-29 1.0090816614512903 2314 30-31 1.0025100172569168 2314 32-33 0.9707758727250209 2314 34-35 0.9964362810813725 2314 36-37 1.024586626624739 2314 38-39 1.0216612633001034 2314 40-41 1.1683822292517902 2314 42-43 1.2512318300118395 2314 44-45 1.2670846853124758 2314 46-47 1.3544159004166616 2314 48-49 1.2971025842640316 2314 50-51 1.2777828416040649 2314 52-53 1.2284528138096107 2314 54-55 1.048649480118307 2314 56-57 1.0094998339299508 2314 58-59 1.0406269274473772 2314 60-61 1.071150368209338 2314 62-63 1.1034097704400168 2314 64-65 1.1249330537312403 2314 66-67 1.1965292769268245 2314 68-69 1.1005166568587441 2314 70-71 1.1637155672736483 2314 72-73 1.1835986920054111 2314 74-75 1.1401328376521533 2314 76-77 1.195946301493187 2314 78-79 1.178741348007886 2314 80-81 1.0890241424690394 2314 82-83 1.1286627539088165 2314 84-85 1.2102920633430756 2314 86-87 1.205914986068887 2314 88-89 1.2608653158487293 2314 90-91 1.3168475410773688 2314 92-93 1.3550291096567229 2314 94-95 1.3437609562938633 2314 96-97 1.3303398578373375 2314 98-99 1.4516808122384184 2314 100 1.5153351853029697 2315 1 0.4330651775436998 2315 2 0.49119518316581434 2315 3 0.5657312861480328 2315 4 0.2880203405941728 2315 5 0.33693605649872893 2315 6 0.44090654495654746 2315 7 0.4307298641982129 2315 8 0.4766147433639958 2315 9 0.6855287470993261 2315 10-11 0.5499436086518017 2315 12-13 0.5793710865420252 2315 14-15 0.803265778358309 2315 16-17 0.7573275361457945 2315 18-19 0.6163616426233389 2315 20-21 0.7480495316744182 2315 22-23 0.6179638774275205 2315 24-25 0.8080829538866823 2315 26-27 0.8238935327137966 2315 28-29 0.8811588581371055 2315 30-31 0.8750368888822919 2315 32-33 0.8734403920094493 2315 34-35 0.896019547549912 2315 36-37 0.9933084908927228 2315 38-39 1.0431266145385436 2315 40-41 1.0714245590390092 2315 42-43 1.184467374163468 2315 44-45 1.0997223834268723 2315 46-47 1.2464489659820046 2315 48-49 1.1510253829815795 2315 50-51 1.0893157376322904 2315 52-53 1.0514855579211968 2315 54-55 0.9457374100842415 2315 56-57 0.9036414416923577 2315 58-59 0.9056452073612249 2315 60-61 0.9214928551897614 2315 62-63 0.9163107422157424 2315 64-65 1.0121370829182865 2315 66-67 1.0109042447413472 2315 68-69 0.9976513911800993 2315 70-71 0.908069669203762 2315 72-73 0.6806598273756563 2315 74-75 0.8932749660561612 2315 76-77 0.9268068154883267 2315 78-79 0.9412365068694122 2315 80-81 0.6709261323771578 2315 82-83 0.6765860087473747 2315 84-85 0.9508338667309282 2315 86-87 1.068462151631838 2315 88-89 1.1357255821039836 2315 90-91 1.1629640795651426 2315 92-93 1.2439416534322625 2315 94-95 1.2098783365418058 2315 96-97 1.0287498169231526 2315 98-99 0.4490051413075591 2315 100 1.0048088977384921 2316 1 0.17082496524052004 2316 2 0.10582834493557414 2316 3 0.1693180539780208 2316 4 0.010848303760113254 2316 5 -0.0407990603827173 2316 6 0.08812096877525732 2316 7 -0.028327489231735115 2316 8 0.06386236183750071 2316 9 0.056801953979288555 2316 10-11 0.07505857141454442 2316 12-13 -0.151979144509383 2316 14-15 -0.16684084234749008 2316 16-17 -0.01371670689668747 2316 18-19 0.08572424520437494 2316 20-21 0.06591871489872858 2316 22-23 0.05458055821066665 2316 24-25 0.13291237741102435 2316 26-27 0.13890837906537712 2316 28-29 0.1423744857077125 2316 30-31 -0.039950795647456516 2316 32-33 0.18468723925610675 2316 34-35 0.2678241691409369 2316 36-37 0.278052378134106 2316 38-39 0.405957467459622 2316 40-41 0.4664254233854166 2316 42-43 0.23522077461010582 2316 44-45 0.18282328537293324 2316 46-47 0.11470507616048309 2316 48-49 0.002068697174195222 2316 50-51 0.12841869852888976 2316 52-53 0.21375659115173562 2316 54-55 -0.06749865359076068 2316 56-57 -0.1344191080601007 2316 58-59 -0.043102956342472964 2316 60-61 -0.03002792536312171 2316 62-63 0.05645213849757624 2316 64-65 0.10231465977885534 2316 66-67 0.10401664706844826 2316 68-69 0.042996105895355186 2316 70-71 -0.1785656354883116 2316 72-73 -0.2849528018863552 2316 74-75 -0.07395775563579576 2316 76-77 0.0968635574254435 2316 78-79 0.10017866884253479 2316 80-81 0.2564023567525737 2316 82-83 0.3541786081595255 2316 84-85 0.4273659353898225 2316 86-87 0.42306636190186353 2316 88-89 0.4002729659959918 2316 90-91 0.2652509618829164 2316 92-93 0.2637296973987624 2316 94-95 0.49335701767820694 2316 96-97 0.470314241691824 2316 98-99 0.3493899337834989 2316 100 0.14833114334093978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 32.0 1 472.0 2 177.0 3 162.0 4 128.0 5 121.0 6 120.0 7 557.0 8 1692.0 9 3416.0 10 6132.0 11 11262.0 12 17935.0 13 23538.0 14 27536.0 15 31218.0 16 36238.0 17 41994.0 18 49163.0 19 57263.0 20 67162.0 21 78401.0 22 85732.0 23 87749.0 24 89178.0 25 94480.0 26 104703.0 27 118683.0 28 137504.0 29 162467.0 30 194210.0 31 237188.0 32 294688.0 33 380621.0 34 522156.0 35 713794.0 36 1039517.0 37 1406898.0 38 1229378.0 39 322854.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.659584251784594 19.852793204051544 12.983764006164487 23.503858537999378 2 15.574636706679692 19.520313252037717 41.26140814721385 23.643641894068743 3 19.442228168962707 25.297672867316173 28.69851502304724 26.561583940673884 4 12.586192205981101 15.928390018315234 38.58072968576438 32.904688089939285 5 13.317621547026828 37.7703820885115 34.67579685699999 14.236199507461686 6 31.109976076517608 37.14575495034666 17.63190367631304 14.112365296822697 7 27.97715659988585 31.844857673850456 22.099201385535423 18.07878434072827 8 26.19385659515339 35.67058000483248 19.64689771009637 18.488665689917756 9 26.205225018905402 15.771672014129237 21.043721484706335 36.97938148225903 10-11 25.724515677728004 26.151161299671504 27.287968593461194 20.8363544291393 12-13 25.713579034501166 23.647968545079618 27.946188233282665 22.69226418713655 14-15 23.022089065011247 25.116950462460157 25.626769272300127 26.23419120022847 16-17 22.092534009472033 28.00618535842176 26.695881094053835 23.205399538052376 18-19 21.64959903819195 27.641252528810988 28.815573202368455 21.893575230628603 20-21 22.812377446988698 26.87049281782352 28.535969512393734 21.781160222794046 22-23 22.995012249991156 26.664328743677363 28.203852986555468 22.136806019776014 24-25 22.5148732132714 26.58158083131557 28.827053134054005 22.07649282135903 26-27 22.453619122992926 26.902117071989256 28.125384141792953 22.518879663224865 28-29 21.932071068546612 27.129627229002338 28.56699328757106 22.37130841487999 30-31 22.639054709904567 26.872756204977534 28.476621799017458 22.011567286100444 32-33 22.663602559523156 27.07430644402553 27.924536242289435 22.337554754161886 34-35 22.741062928195806 26.990047401454138 28.393452785710018 21.875436884640045 36-37 22.50344019820545 26.75643917873545 28.10024512491195 22.63987549814715 38-39 22.587058337167097 26.95376957580538 28.42681395612143 22.032358130906097 40-41 22.502507183437228 26.309510671539492 28.715305786476442 22.472676358546842 42-43 22.448018134152964 26.423672648099988 28.312318983253192 22.815990234493853 44-45 22.483073995765885 27.04745080272724 28.196321645522914 22.273153555983964 46-47 22.46328181054426 26.898370322758808 27.748373512429257 22.88997435426767 48-49 22.882079777873326 26.87030233119244 27.72703345446163 22.520584436472607 50-51 22.661446169864718 26.802852236786652 27.764277878291388 22.771423715057242 52-53 22.408831538311578 27.157336063309312 27.548158569348026 22.885673829031084 54-55 22.266959470971553 27.095396281046526 27.674267915938973 22.963376332042948 56-57 22.856488134909416 26.85991167877119 27.317735089610988 22.965865096708406 58-59 22.297139154621807 27.07541216982627 27.86459202120118 22.762856654350745 60-61 22.39554085637384 27.481066711000473 27.43486539469292 22.688527037932765 62-63 22.537208078919466 27.030562237680233 27.297099478332814 23.135130205067483 64-65 22.151936461770998 27.038223172732916 27.528168466757098 23.281671898738992 66-67 22.427428221768942 26.87675860607771 27.403640049087862 23.292173123065485 68-69 22.1600589219995 26.896004547377245 27.60405630845646 23.339880222166794 70-71 22.123949563953225 26.638117272487733 27.66434080585187 23.573592357707177 72-73 22.483599382830242 26.716603577637628 27.71602814939449 23.083768890137645 74-75 22.617050436685616 26.877923406608417 27.45024447215273 23.054781684553234 76-77 22.419322245759428 26.75477722815169 27.315183903105527 23.510716622983356 78-79 22.35688044498253 26.73369937491511 27.3417557423221 23.56766443778026 80-81 22.081549728091137 27.067915848316833 27.60615051626072 23.24438390733131 82-83 22.673302143275272 26.5011871183297 28.214864053030308 22.610646685364717 84-85 22.723942366905824 26.72290040942233 27.477283880782245 23.075873342889604 86-87 21.94581177141608 27.125707242394014 27.802753941204283 23.125727044985624 88-89 22.555442047870304 26.32813437445402 27.75691761014049 23.35950596753518 90-91 22.482747541686738 26.422980103795524 27.715394989344368 23.378877365173377 92-93 22.602923600185555 26.49517472490398 27.712380602591498 23.189521072318968 94-95 21.9319735176857 26.467799800770926 28.12711703695249 23.473109644590885 96-97 22.166626047713297 26.358606270224378 27.831188281259312 23.64357940080301 98-99 21.82749605915913 26.59484507524537 27.749278869770226 23.828379995825273 100 22.39623393557199 26.20078875330097 27.229462008851748 24.173515302275298 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 753.0 1 560.5 2 616.0 3 890.5 4 770.5 5 703.0 6 1032.0 7 1813.5 8 2876.0 9 3476.5 10 3346.0 11 3244.5 12 3616.0 13 4178.5 14 4803.5 15 5589.5 16 6398.0 17 7132.5 18 7896.0 19 8675.0 20 9582.5 21 10565.5 22 12053.0 23 14347.0 24 17372.5 25 20876.5 26 25770.0 27 32301.5 28 40421.0 29 52883.0 30 69212.5 31 85839.0 32 107657.5 33 131746.0 34 151479.0 35 171886.0 36 194905.5 37 224043.5 38 239108.0 39 238860.5 40 234895.0 41 227059.0 42 228158.0 43 237866.5 44 252385.5 45 279326.0 46 304187.5 47 320252.0 48 333881.5 49 334583.5 50 325411.5 51 327160.0 52 322697.0 53 304258.5 54 285615.0 55 253466.0 56 223523.0 57 189999.0 58 157824.5 59 143503.5 60 120063.0 61 90595.5 62 69936.0 63 55996.5 64 41702.5 65 26062.0 66 17386.5 67 12628.0 68 9405.5 69 7128.0 70 5475.0 71 4145.5 72 3054.0 73 2282.5 74 1756.5 75 1249.5 76 882.0 77 591.5 78 379.5 79 258.0 80 194.0 81 150.5 82 121.5 83 91.5 84 69.5 85 56.5 86 45.5 87 33.0 88 31.0 89 30.5 90 23.0 91 20.0 92 14.0 93 6.5 94 6.0 95 5.0 96 4.5 97 4.0 98 3.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058787140400963986 2 0.13259346380266174 3 0.017051266737173026 4 0.016556271980860897 5 0.019630449941115163 6 0.05279509861402771 7 0.05778412471054205 8 0.04917382118627057 9 0.04058957001759446 10-11 0.03950839726038639 12-13 0.0408631197513459 14-15 0.01793704682741578 16-17 0.01697962275928574 18-19 0.016999162025982277 20-21 0.02082885829850242 22-23 0.02534242890540116 24-25 0.024665067659921405 26-27 0.01902473267352269 28-29 0.018054282427594968 30-31 0.01611338193573952 32-33 0.022763245701459024 34-35 0.023030282346311617 36-37 0.023219161924378087 38-39 0.021128460387849234 40-41 0.024775790171201753 42-43 0.032435182716242045 44-45 0.04204198884203662 46-47 0.047447852628076964 48-49 0.039462805638094484 50-51 0.027361486464042732 52-53 0.03950839726038639 54-55 0.036232313544267966 56-57 0.03816018785832573 58-59 0.043155727043738906 60-61 0.02270462790136943 62-63 0.025225193305221973 64-65 0.024886512682482095 66-67 0.03187505707094147 68-69 0.03875287894812051 70-71 0.04078496268455977 72-73 0.04472538146836026 74-75 0.026684125218562983 76-77 0.019930052030461977 78-79 0.019428544185251004 80-81 0.024111455103519686 82-83 0.020060313808438854 84-85 0.011879874151491061 86-87 0.014146429088288698 88-89 0.01826270127235797 90-91 0.020398994431178732 92-93 0.019324334762869506 94-95 0.02298469072401971 96-97 0.024235203792597717 98-99 0.0272377377749647 100 0.028735748221698772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7676849.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.94423182434505 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83313305751322 52.77423534841604 2 13.283769859948174 17.785435380112364 3 4.075078472674119 8.184089939312827 4 1.5753781021633002 4.218499075288668 5 0.7602715637540457 2.5447897906704076 6 0.4109361865488045 1.6505884402401279 7 0.25046406579045705 1.1736987138761055 8 0.16233587719188874 0.8693960476913766 9 0.11507496200741887 0.6933244440422113 >10 0.4924027789198499 5.57935063735808 >50 0.024971100244573014 1.1589766537697521 >100 0.014703365248300635 1.8310568830954708 >500 0.0011104638422280255 0.49466710062357766 >1k 2.961153230082752E-4 0.3084326721240134 >5k 2.4676276872319948E-5 0.15766061908850054 >10k+ 4.9352553744639895E-5 0.5757982542905254 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19454 0.25341126287621396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15402 0.20062919043998392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9544 0.12432184090113013 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3026177797687567E-5 2 0.0 0.0 0.0 0.0 1.3026177797687567E-5 3 0.0 0.0 0.0 0.0 1.3026177797687567E-5 4 0.0 0.0 0.0 0.0 1.3026177797687567E-5 5 0.0 0.0 0.0 0.0 1.3026177797687567E-5 6 0.0 0.0 0.0 0.0 1.3026177797687567E-5 7 0.0 0.0 0.0 0.0 1.3026177797687567E-5 8 0.0 0.0 0.0 0.0 1.3026177797687567E-5 9 0.0 0.0 0.0 0.0 1.3026177797687567E-5 10-11 0.0 0.0 0.0 0.0 1.3026177797687567E-5 12-13 0.0 0.0 0.0 1.3026177797687567E-5 2.6052355595375134E-5 14-15 0.0 0.0 0.0 1.3026177797687567E-5 3.9078533393062704E-5 16-17 0.0 0.0 0.0 1.9539266696531352E-5 6.513088898843783E-5 18-19 0.0 0.0 0.0 3.9078533393062704E-5 1.0420942238150053E-4 20-21 0.0 0.0 0.0 5.210471119075027E-5 1.172356001791881E-4 22-23 0.0 2.6052355595375134E-5 0.0 1.953926669653135E-4 1.7585340026878216E-4 24-25 0.0 2.6052355595375134E-5 0.0 6.057172675924718E-4 2.2144502256068863E-4 26-27 1.3026177797687567E-5 5.210471119075027E-5 0.0 0.0012635392463756939 2.4749737815606376E-4 28-29 1.3026177797687567E-5 5.210471119075027E-5 0.0 0.004057654383979677 2.4749737815606376E-4 30-31 1.3026177797687567E-5 6.513088898843783E-5 0.0 0.01592450235767305 2.605235559537513E-4 32-33 1.3026177797687567E-5 6.513088898843783E-5 0.0 0.043553025466568376 2.8657591154912645E-4 34-35 1.3026177797687567E-5 6.513088898843783E-5 0.0 0.08352385203877269 2.99602089346814E-4 36-37 1.3026177797687567E-5 6.513088898843783E-5 0.0 0.15007459440715848 3.1262826714450163E-4 38-39 1.3026177797687567E-5 6.513088898843783E-5 0.0 0.24563463473099445 3.582198894364081E-4 40-41 1.9539266696531352E-5 6.513088898843783E-5 0.0 0.3464572508850962 3.647329783352519E-4 42-43 2.6052355595375134E-5 6.513088898843783E-5 0.0 0.4455017937698136 3.7775915613293945E-4 44-45 2.6052355595375134E-5 6.513088898843783E-5 0.0 0.5632584410609092 3.90785333930627E-4 46-47 2.6052355595375134E-5 6.513088898843783E-5 0.0 0.7036024806531951 3.972984228294708E-4 48-49 2.6052355595375134E-5 6.513088898843783E-5 0.0 0.8545107504394056 4.038115117283146E-4 50-51 2.6052355595375134E-5 7.164397788728163E-5 0.0 1.0087146432084309 4.038115117283146E-4 52-53 2.6052355595375134E-5 7.815706678612541E-5 0.0 1.1579946407699304 4.624293118179086E-4 54-55 2.6052355595375134E-5 7.815706678612541E-5 0.0 1.3219811930650192 4.8196857851443996E-4 56-57 2.6052355595375134E-5 9.118324458381297E-5 0.0 1.5137786349581708 4.884816674132837E-4 58-59 2.6052355595375134E-5 1.0420942238150053E-4 0.0 1.7337582125166198 4.949947563121275E-4 60-61 2.6052355595375134E-5 1.0420942238150053E-4 0.0 1.9523635283174126 4.949947563121275E-4 62-63 2.6052355595375134E-5 1.0420942238150053E-4 0.0 2.1745770953681647 4.949947563121275E-4 64-65 2.6052355595375134E-5 1.0420942238150053E-4 0.0 2.4015973220262636 4.949947563121275E-4 66-67 2.6052355595375134E-5 1.0420942238150053E-4 0.0 2.655125820502657 4.949947563121275E-4 68-69 2.6052355595375134E-5 1.0420942238150053E-4 0.0 2.9341139834846306 4.949947563121275E-4 70-71 2.6052355595375134E-5 1.0420942238150053E-4 0.0 3.236145454990713 5.470994675028778E-4 72-73 2.6052355595375134E-5 1.0420942238150053E-4 0.0 3.535415376803686 6.447958009855346E-4 74-75 2.6052355595375134E-5 1.0420942238150053E-4 0.0 3.828015895584243 6.513088898843784E-4 76-77 2.6052355595375134E-5 1.0420942238150053E-4 0.0 4.15701155513154 6.578219787832222E-4 78-79 2.6052355595375134E-5 1.0420942238150053E-4 0.0 4.513609685432135 6.64335067682066E-4 80-81 2.6052355595375134E-5 1.0420942238150053E-4 0.0 4.894156443613779 6.773612454797535E-4 82-83 2.6052355595375134E-5 1.0420942238150053E-4 0.0 5.281073002738493 7.099266899739724E-4 84-85 2.6052355595375134E-5 1.172356001791881E-4 0.0 5.652898734884586 7.164397788728162E-4 86-87 2.6052355595375134E-5 1.172356001791881E-4 0.0 6.04550773370689 7.164397788728162E-4 88 2.6052355595375134E-5 1.172356001791881E-4 0.0 6.363274827992579 7.164397788728162E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11875 0.0 47.522552 1 GTATCAA 21270 0.0 41.136383 1 CAACGCA 23915 0.0 34.55609 5 ATCAACG 24000 0.0 34.492466 3 GTACTAG 5325 0.0 33.145596 1 TATCAAC 26390 0.0 32.72671 2 AACGCAG 26915 0.0 31.315693 6 TCAACGC 27480 0.0 30.346807 4 GCATCGT 5220 0.0 29.446299 8 ACGCAGA 29050 0.0 28.30199 7 CTGTGCG 6640 0.0 28.104319 9 CGCAGAG 29510 0.0 27.811762 8 GTACATG 28910 0.0 27.399979 1 TAGGCAT 6870 0.0 27.02816 5 TACCTGG 4640 0.0 26.778831 2 TAGTACT 7085 0.0 26.672415 4 TACATGG 29395 0.0 26.402977 2 CTAGTAC 7770 0.0 25.772984 3 CTAGGCA 7720 0.0 25.696339 4 ACATGGG 29200 0.0 25.532682 3 >>END_MODULE