FastQCFastQC Report
Mon 6 Jun 2016
ERR1211213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7676849
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183880.239525357343879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157610.20530558826935374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96160.12525972570256366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126000.046.233551
GTATCAA217150.038.10741
CAACGCA246150.031.9345725
ATCAACG248550.031.7686793
TATCAAC275250.029.7257372
AACGCAG277950.028.9740476
TACCTGG48600.028.4296972
TAGGCAT70850.028.3836945
TCAACGC283750.028.0927074
CTGTGCG72800.027.5592359
GGGCACC66200.027.34079494
GCATCGT63250.026.6688698
GTGGTAT49150.026.3066561
CTAGGCA79450.026.0884534
ACGCAGA304700.025.8752467
TAGTACT79650.025.6688924
GTACATG296350.025.4957281
CGCAGAG309600.025.3446438
CGACGAG33550.024.64691224-25
CTAGTAC85000.024.4403253
Produced by FastQC (version 0.11.5)