##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7676849 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16151600741398 33.0 31.0 34.0 30.0 34.0 2 32.30720156147399 34.0 31.0 34.0 31.0 34.0 3 32.38593425505699 34.0 31.0 34.0 30.0 34.0 4 35.99677953806308 37.0 35.0 37.0 35.0 37.0 5 35.88143651125612 37.0 35.0 37.0 35.0 37.0 6 35.90662887859329 37.0 35.0 37.0 35.0 37.0 7 35.84238064341242 37.0 35.0 37.0 35.0 37.0 8 35.8280185008198 37.0 35.0 37.0 35.0 37.0 9 37.43664946386206 39.0 37.0 39.0 34.0 39.0 10-11 37.416088228386414 39.0 37.0 39.0 34.0 39.0 12-13 37.31993386870056 39.0 37.0 39.0 34.0 39.0 14-15 38.557828153191494 40.0 38.0 41.0 34.0 41.0 16-17 38.510677818464316 40.0 38.0 41.0 34.0 41.0 18-19 38.5253856106848 40.0 38.0 41.0 34.0 41.0 20-21 38.485599299921105 40.0 38.0 41.0 34.0 41.0 22-23 38.359570899466696 40.0 38.0 41.0 34.0 41.0 24-25 38.300395253312914 40.0 38.0 41.0 34.0 41.0 26-27 38.16700413151281 40.0 38.0 41.0 33.5 41.0 28-29 38.14755989078331 40.0 38.0 41.0 33.5 41.0 30-31 38.16879125797577 40.0 38.0 41.0 34.0 41.0 32-33 38.05233045485198 40.0 37.0 41.0 33.0 41.0 34-35 37.92228282723811 40.0 37.0 41.0 33.0 41.0 36-37 37.78428818907341 40.0 37.0 41.0 33.0 41.0 38-39 37.69903908491622 40.0 37.0 41.0 33.0 41.0 40-41 37.60247941570819 40.0 37.0 41.0 32.5 41.0 42-43 37.46713000346887 40.0 36.5 41.0 32.0 41.0 44-45 37.33161737322175 40.0 36.0 41.0 32.0 41.0 46-47 37.1061423769049 39.0 36.0 41.0 31.0 41.0 48-49 36.88078403001023 39.0 35.5 41.0 31.0 41.0 50-51 36.68674191715898 39.0 35.0 41.0 30.5 41.0 52-53 36.48387241953046 39.0 35.0 40.0 30.0 41.0 54-55 36.24658072602444 38.5 35.0 40.0 30.0 41.0 56-57 35.99149071448455 38.0 35.0 40.0 29.0 41.0 58-59 35.71669411499432 38.0 34.5 40.0 29.0 41.0 60-61 35.45757699545738 37.5 34.0 40.0 28.0 41.0 62-63 35.15581119284748 37.0 34.0 40.0 28.0 41.0 64-65 34.7831526320239 36.0 34.0 39.0 27.5 41.0 66-67 34.480590408903446 36.0 33.5 39.0 27.0 40.5 68-69 34.151783107887105 35.5 33.0 39.0 26.0 40.0 70-71 33.83827759279882 35.0 33.0 38.0 26.0 40.0 72-73 33.503768864022206 35.0 33.0 37.5 26.0 40.0 74-75 33.16903198174147 35.0 32.0 37.0 26.0 39.0 76-77 32.1788946871301 34.0 31.0 36.0 24.5 38.5 78-79 32.674012475691526 35.0 32.0 36.0 26.0 38.5 80-81 32.59902044445579 35.0 32.0 36.0 26.0 37.5 82-83 32.35035025438171 35.0 32.0 35.0 25.0 37.0 84-85 32.10185487561368 34.0 32.0 35.0 25.0 36.5 86-87 31.871045333834232 34.0 31.0 35.0 25.0 36.0 88-89 31.65821888642072 34.0 31.0 35.0 24.5 36.0 90-91 31.44814767100408 34.0 31.0 35.0 24.0 35.5 92-93 31.269297077485827 34.0 31.0 35.0 24.0 35.0 94-95 31.08638081848425 34.0 31.0 35.0 23.0 35.0 96-97 30.921743152691946 34.0 30.5 35.0 23.0 35.0 98-99 30.752010036930514 34.0 30.0 35.0 20.0 35.0 100 28.931391251801358 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33373276385913186 1101 2 -0.3349070250037762 1101 3 -0.3938234261013136 1101 4 -0.20162469316829146 1101 5 -0.23983102641985 1101 6 -0.3067305018201978 1101 7 -0.2760231138584004 1101 8 -0.3449370780036247 1101 9 -0.4436342770871775 1101 10-11 -0.4718061347823621 1101 12-13 -0.48621040438497687 1101 14-15 -0.6825035501444034 1101 16-17 -0.6071720417666668 1101 18-19 -0.6768626618211186 1101 20-21 -0.7152108554632051 1101 22-23 -0.7572691066492325 1101 24-25 -0.6674169426805463 1101 26-27 -0.7855224780694172 1101 28-29 -0.827924931699215 1101 30-31 -0.6995748436648768 1101 32-33 -0.6939640368545525 1101 34-35 -0.6468370869246414 1101 36-37 -0.558083230959042 1101 38-39 -0.594335657893609 1101 40-41 -0.7358181957078997 1101 42-43 -0.7480003289475121 1101 44-45 -0.752787530018395 1101 46-47 -0.7708612836344457 1101 48-49 -0.7707151655075464 1101 50-51 -0.8495034660289278 1101 52-53 -0.9320644657709067 1101 54-55 -0.7804009582439946 1101 56-57 -0.8133053855996621 1101 58-59 -0.7330969176424489 1101 60-61 -0.8306161739773543 1101 62-63 -0.8531272472879365 1101 64-65 -0.8826729245802625 1101 66-67 -0.9156997215612392 1101 68-69 -0.9942418924939886 1101 70-71 -0.97001735967347 1101 72-73 -1.08935827376623 1101 74-75 -1.0812321230796087 1101 76-77 -0.9834265251943393 1101 78-79 -0.8217328842181182 1101 80-81 -0.7725591651455161 1101 82-83 -0.8604625173720919 1101 84-85 -0.8396045528280567 1101 86-87 -0.9365452222035735 1101 88-89 -0.8139338512339229 1101 90-91 -0.982007435390841 1101 92-93 -0.9851815913116404 1101 94-95 -1.0319992920944863 1101 96-97 -1.0608111427784515 1101 98-99 -1.165282733890038 1101 100 -1.0661167831998952 1102 1 -0.426897174363571 1102 2 -0.35799010867644654 1102 3 -0.3355106244951571 1102 4 -0.26475887475172044 1102 5 -0.350755249274016 1102 6 -0.39043397080470044 1102 7 -0.3415287294169218 1102 8 -0.40006642440900464 1102 9 -0.4993301024086847 1102 10-11 -0.505581705488126 1102 12-13 -0.4984060375658288 1102 14-15 -0.6216701164328029 1102 16-17 -0.680853904460399 1102 18-19 -0.6349964867615014 1102 20-21 -0.7346429718832823 1102 22-23 -0.8168965159962838 1102 24-25 -0.735029806721343 1102 26-27 -0.743618384670981 1102 28-29 -0.7793884363303221 1102 30-31 -0.6994425191952942 1102 32-33 -0.6685396432203987 1102 34-35 -0.6849028859438775 1102 36-37 -0.8388018251897194 1102 38-39 -0.8335181351382914 1102 40-41 -0.7791618904379689 1102 42-43 -0.7099389116029826 1102 44-45 -0.7444168460680487 1102 46-47 -0.7688118290202368 1102 48-49 -0.8267969995181659 1102 50-51 -0.8227068393117563 1102 52-53 -0.9067662770077689 1102 54-55 -0.8751243285843628 1102 56-57 -0.8900167129070979 1102 58-59 -0.9901508978104019 1102 60-61 -0.8673536830551072 1102 62-63 -0.9180946646913597 1102 64-65 -0.8858637843899757 1102 66-67 -0.9744155951089724 1102 68-69 -1.0616925608405268 1102 70-71 -1.0604885885651214 1102 72-73 -0.9915979815388667 1102 74-75 -1.1275856702159217 1102 76-77 -0.9049802337616555 1102 78-79 -0.7046743483724178 1102 80-81 -0.7692311041465416 1102 82-83 -0.79696643684332 1102 84-85 -0.83288416977868 1102 86-87 -0.740623861973333 1102 88-89 -0.9070606488528377 1102 90-91 -0.8958858718087583 1102 92-93 -1.0577071658618955 1102 94-95 -1.0230849223627914 1102 96-97 -1.0776370264432167 1102 98-99 -1.1600235550925575 1102 100 -1.223654125285286 1103 1 -0.35241034364635127 1103 2 -0.3610703970347302 1103 3 -0.3801619774239029 1103 4 -0.24473136899688797 1103 5 -0.2968591482293945 1103 6 -0.3886382981306653 1103 7 -0.4289972574711385 1103 8 -0.42699210170284374 1103 9 -0.5123838291400205 1103 10-11 -0.5478116164026261 1103 12-13 -0.6002836261412483 1103 14-15 -0.7272076744659657 1103 16-17 -0.6995159418916188 1103 18-19 -0.7293205948839017 1103 20-21 -0.7615096716798107 1103 22-23 -0.7400653043484269 1103 24-25 -0.8778299411924735 1103 26-27 -0.9147544823717126 1103 28-29 -0.8896931489271438 1103 30-31 -0.8621730224056918 1103 32-33 -0.8066741209408121 1103 34-35 -0.8720815846298962 1103 36-37 -0.8362279077429235 1103 38-39 -0.7525937611637872 1103 40-41 -0.8400019971366177 1103 42-43 -0.8087892021154417 1103 44-45 -0.7475679745122932 1103 46-47 -0.9466972720727753 1103 48-49 -0.9396267052779876 1103 50-51 -1.006210168521342 1103 52-53 -1.0221386834108301 1103 54-55 -0.9467154068767982 1103 56-57 -0.9946432302307926 1103 58-59 -1.040202412143536 1103 60-61 -1.0230301543393452 1103 62-63 -0.8640943721011709 1103 64-65 -0.9412340280814959 1103 66-67 -1.037794956506083 1103 68-69 -1.0443750111134023 1103 70-71 -1.1242149942117408 1103 72-73 -1.0248219310298907 1103 74-75 -1.0461560434831014 1103 76-77 -1.0599283197395835 1103 78-79 -0.8855124781762598 1103 80-81 -0.8110811174383308 1103 82-83 -0.848282928024993 1103 84-85 -0.9008121782795726 1103 86-87 -0.9800971512457402 1103 88-89 -1.005170701635361 1103 90-91 -0.943970331081676 1103 92-93 -1.0283578712376418 1103 94-95 -1.0539364862687428 1103 96-97 -1.119014640349885 1103 98-99 -1.0986603356799236 1103 100 -1.2354258995699254 1104 1 -0.3786782874058865 1104 2 -0.34460452893658555 1104 3 -0.43022935197509327 1104 4 -0.29359869835062824 1104 5 -0.3416747891477314 1104 6 -0.4122615528430913 1104 7 -0.37314781411527065 1104 8 -0.4084300093020161 1104 9 -0.5728458935213183 1104 10-11 -0.5760059025395634 1104 12-13 -0.6210567735097356 1104 14-15 -0.8016763695728102 1104 16-17 -0.7305664789485817 1104 18-19 -0.7485609501319743 1104 20-21 -0.7875360283440429 1104 22-23 -0.7330397636956576 1104 24-25 -0.7503192363122864 1104 26-27 -0.8086403171403589 1104 28-29 -0.917268168616701 1104 30-31 -0.9138812738547912 1104 32-33 -0.9038976280939863 1104 34-35 -0.9217414955311796 1104 36-37 -0.8579715594411894 1104 38-39 -0.8692244638178366 1104 40-41 -0.8984521306931512 1104 42-43 -0.8385038125271862 1104 44-45 -0.9471864042287024 1104 46-47 -0.9111078971346629 1104 48-49 -1.034375345078196 1104 50-51 -0.9745161822150123 1104 52-53 -0.9455615973113751 1104 54-55 -1.011022988507456 1104 56-57 -0.9678990087459596 1104 58-59 -1.0149892059605463 1104 60-61 -1.0661093616590236 1104 62-63 -1.049114068887981 1104 64-65 -0.9433844905241315 1104 66-67 -0.9797168405746319 1104 68-69 -1.013722059799676 1104 70-71 -1.0502649193203482 1104 72-73 -1.0542241225278062 1104 74-75 -1.1295402118970728 1104 76-77 -1.0310307146019149 1104 78-79 -0.8321877735557806 1104 80-81 -0.8586894353103318 1104 82-83 -0.8805580544682208 1104 84-85 -0.9415458947311315 1104 86-87 -0.9387415181767231 1104 88-89 -1.0509343204377863 1104 90-91 -0.9739076461269072 1104 92-93 -0.9741940263998004 1104 94-95 -1.0222468667199287 1104 96-97 -1.0590522836066363 1104 98-99 -1.0745234599365183 1104 100 -1.1619683045800002 1105 1 -0.4140508786766155 1105 2 -0.3838721823955247 1105 3 -0.43245463055924205 1105 4 -0.3293669493475164 1105 5 -0.3394865155735971 1105 6 -0.36580665079481633 1105 7 -0.4245032710467811 1105 8 -0.423716796930087 1105 9 -0.6277558106121077 1105 10-11 -0.5824159259672754 1105 12-13 -0.5616031569807234 1105 14-15 -0.7844388721382742 1105 16-17 -0.7218918328742205 1105 18-19 -0.7629961127363032 1105 20-21 -0.8333191725080056 1105 22-23 -0.778794359424019 1105 24-25 -0.7738453663475298 1105 26-27 -0.8479160973073263 1105 28-29 -0.8371820698935721 1105 30-31 -0.8034099535772086 1105 32-33 -0.8276613698774611 1105 34-35 -0.7681870773548951 1105 36-37 -0.7506797911906773 1105 38-39 -0.7780776455709741 1105 40-41 -0.8429471946326501 1105 42-43 -0.8437548054549708 1105 44-45 -0.8672318322267216 1105 46-47 -0.8092294760454735 1105 48-49 -0.8941106734913618 1105 50-51 -0.8485423117104745 1105 52-53 -0.9012929296632706 1105 54-55 -0.8943906039537879 1105 56-57 -0.8957037036201569 1105 58-59 -1.0160949480465433 1105 60-61 -0.9371700523944142 1105 62-63 -0.975115054408775 1105 64-65 -1.0427962308163714 1105 66-67 -0.977414163343127 1105 68-69 -0.9199448570689555 1105 70-71 -0.9773311033505152 1105 72-73 -1.0293538469790278 1105 74-75 -1.0211598846000527 1105 76-77 -0.9386728669188535 1105 78-79 -0.8393639866153961 1105 80-81 -0.8181160207890841 1105 82-83 -0.8053742199359135 1105 84-85 -0.8430463549247165 1105 86-87 -0.9240427923365857 1105 88-89 -0.9164333141054293 1105 90-91 -0.8613338775091002 1105 92-93 -0.8854334615294199 1105 94-95 -0.9082971654649157 1105 96-97 -0.9192857702258799 1105 98-99 -1.0382950153548514 1105 100 -1.045478572240775 1106 1 -0.4519519114393482 1106 2 -0.41101703929563627 1106 3 -0.4446498279371873 1106 4 -0.2897411960423213 1106 5 -0.31073986483566074 1106 6 -0.36886798366760587 1106 7 -0.43187310463125783 1106 8 -0.4367254396230109 1106 9 -0.5563902870151125 1106 10-11 -0.569259319248637 1106 12-13 -0.6739248612218063 1106 14-15 -0.7517168776307841 1106 16-17 -0.7240051373973913 1106 18-19 -0.7208350779206327 1106 20-21 -0.8043498323431066 1106 22-23 -0.697611381091356 1106 24-25 -0.7998882837651706 1106 26-27 -0.7899525283341404 1106 28-29 -0.8407597900795665 1106 30-31 -0.8560377185508727 1106 32-33 -0.8542684298786583 1106 34-35 -0.7547412033448353 1106 36-37 -0.7604201119467149 1106 38-39 -0.7428814760788924 1106 40-41 -0.8493232071425041 1106 42-43 -0.8338635768551939 1106 44-45 -0.8915177957811906 1106 46-47 -0.9489344269764928 1106 48-49 -0.9002923642753373 1106 50-51 -0.9236420833534567 1106 52-53 -0.9504513604786098 1106 54-55 -0.8880178326173294 1106 56-57 -0.8804369740806735 1106 58-59 -0.9802031156964475 1106 60-61 -0.9516326438415987 1106 62-63 -1.0614713023027562 1106 64-65 -0.9373226501856706 1106 66-67 -0.9139080094550636 1106 68-69 -0.9422596222254356 1106 70-71 -0.8990692548397519 1106 72-73 -1.0064543027772146 1106 74-75 -0.9559111688481039 1106 76-77 -0.9033083348235635 1106 78-79 -0.8216409531482896 1106 80-81 -0.7274558514559146 1106 82-83 -0.6904712630461347 1106 84-85 -0.7648925653507348 1106 86-87 -0.8571855327802105 1106 88-89 -0.8393647823783859 1106 90-91 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1.2529507104813504 2310 78-79 1.1164496188499413 2310 80-81 1.06955459829571 2310 82-83 1.067417101510891 2310 84-85 1.1080244097000644 2310 86-87 1.1383616667945837 2310 88-89 1.1830788954898388 2310 90-91 1.2768681381930485 2310 92-93 1.2634605958593959 2310 94-95 1.271235626121662 2310 96-97 1.3089272970640344 2310 98-99 1.3192086209807492 2310 100 1.4691518057986706 2311 1 0.3750986296180159 2311 2 0.3315382210835125 2311 3 0.4155739564024401 2311 4 0.2899187439346491 2311 5 0.43299437048099776 2311 6 0.44658439498206803 2311 7 0.48625525637126543 2311 8 0.45657798934001903 2311 9 0.6027727927649735 2311 10-11 0.6051310303945101 2311 12-13 0.6746885829723652 2311 14-15 0.7829154521578303 2311 16-17 0.6775194681701677 2311 18-19 0.7647112453678133 2311 20-21 0.6924755777433873 2311 22-23 0.8712283556788165 2311 24-25 0.9112716830452996 2311 26-27 0.38121298642708723 2311 28-29 0.686831942860195 2311 30-31 0.8654421258063181 2311 32-33 0.9120238846330437 2311 34-35 0.9276681158153082 2311 36-37 0.9469121747619837 2311 38-39 0.9768286786581442 2311 40-41 0.9646284777301162 2311 42-43 0.9616923371097528 2311 44-45 0.9698523479269738 2311 46-47 0.6112080214030016 2311 48-49 0.9361872150508574 2311 50-51 1.0212141228158345 2311 52-53 0.9547104474358861 2311 54-55 1.0030348860838743 2311 56-57 1.0664538819061988 2311 58-59 1.112776980168782 2311 60-61 1.1538656944400074 2311 62-63 1.126470297042971 2311 64-65 1.200110224164952 2311 66-67 1.2227437432588815 2311 68-69 1.2129444183668312 2311 70-71 1.2059183972645613 2311 72-73 1.2377595135648463 2311 74-75 1.1943143010225938 2311 76-77 1.1471809435515397 2311 78-79 0.8927732780770299 2311 80-81 0.8548783055739335 2311 82-83 0.9281677982938348 2311 84-85 1.0045942576998002 2311 86-87 1.0190701017245338 2311 88-89 1.051445679582212 2311 90-91 1.1224638640558346 2311 92-93 1.185553851795401 2311 94-95 1.2489563892298072 2311 96-97 1.2304019756184807 2311 98-99 1.2781558197701415 2311 100 1.3875320962446231 2312 1 0.22857416152456267 2312 2 0.3769470635893768 2312 3 0.4697636257551778 2312 4 0.19534768353584298 2312 5 0.36266461626196644 2312 6 0.42849943689287784 2312 7 0.3635460381719682 2312 8 0.4134188689220366 2312 9 0.6293777707522139 2312 10-11 0.6473636054963734 2312 12-13 0.718601665405572 2312 14-15 0.9230971598252395 2312 16-17 0.9056054086546581 2312 18-19 0.8619982620613271 2312 20-21 0.8925307006819168 2312 22-23 1.0240870109595122 2312 24-25 0.9657311456772391 2312 26-27 0.978337448411871 2312 28-29 1.0274859387502033 2312 30-31 1.0071773755235327 2312 32-33 0.8773051337039774 2312 34-35 0.9862447413328397 2312 36-37 1.0363993808141316 2312 38-39 0.8126860395895079 2312 40-41 0.9532984628312349 2312 42-43 1.0777553886608686 2312 44-45 0.8167090662022503 2312 46-47 0.8608244913052516 2312 48-49 1.04108991794201 2312 50-51 0.9045240681757676 2312 52-53 1.0612512299769747 2312 54-55 0.9019693271940525 2312 56-57 0.9336733388087453 2312 58-59 1.0975078194281096 2312 60-61 1.1141285101577836 2312 62-63 1.1525253675379403 2312 64-65 1.1532670387693642 2312 66-67 1.160448848782103 2312 68-69 1.186257578341781 2312 70-71 1.0810472548232255 2312 72-73 1.0757811692482946 2312 74-75 1.1642499163169546 2312 76-77 1.1144891630276277 2312 78-79 1.0208194105161397 2312 80-81 0.9506902358388487 2312 82-83 0.9480504673244567 2312 84-85 1.0495710382042063 2312 86-87 1.0279232323059126 2312 88-89 1.0078236042689035 2312 90-91 1.102620794618236 2312 92-93 1.1591191501515432 2312 94-95 1.1786635145547102 2312 96-97 0.8428510542978209 2312 98-99 0.5833712495002068 2312 100 0.8547580914333643 2313 1 0.02604889064683391 2313 2 0.21615900123352816 2313 3 0.27422592090022135 2313 4 0.1646857564724371 2313 5 0.30222757810074796 2313 6 0.2324406036817379 2313 7 0.24123280967705796 2313 8 0.37673536569116806 2313 9 0.6029748175490255 2313 10-11 0.39661617747214706 2313 12-13 0.42602473057227286 2313 14-15 0.4731116736133387 2313 16-17 0.7612480659390215 2313 18-19 0.7001360511347343 2313 20-21 0.6826204553155364 2313 22-23 0.6436253057711028 2313 24-25 0.559109267263878 2313 26-27 0.7683602557931692 2313 28-29 0.5915208145921156 2313 30-31 0.8535746001623465 2313 32-33 0.9393483929151074 2313 34-35 0.95607252102252 2313 36-37 1.004733159464962 2313 38-39 0.9963386406356065 2313 40-41 0.9711897953850865 2313 42-43 1.0090528871165105 2313 44-45 0.8609324716627711 2313 46-47 0.8401317625207128 2313 48-49 0.9795315774301585 2313 50-51 0.9605676822158813 2313 52-53 1.0141166450520842 2313 54-55 0.9546806695377583 2313 56-57 0.6740392829210435 2313 58-59 0.7248946749378931 2313 60-61 0.6006884431250299 2313 62-63 0.9418558736394971 2313 64-65 1.0435522058489326 2313 66-67 0.9779026301689768 2313 68-69 1.0480881966633575 2313 70-71 1.0286075490320385 2313 72-73 1.056516349259887 2313 74-75 1.159735166332446 2313 76-77 1.0702116782093896 2313 78-79 0.9627259109404349 2313 80-81 0.8503312600548583 2313 82-83 0.8958258392001923 2313 84-85 0.887898169155342 2313 86-87 0.9045736894154324 2313 88-89 0.9309201925668127 2313 90-91 0.889468224566226 2313 92-93 1.06472737192394 2313 94-95 1.1287946944038474 2313 96-97 1.1969990265356536 2313 98-99 1.1836313434230128 2313 100 1.2752500212114235 2314 1 -0.36684966858782886 2314 2 0.061015343483333595 2314 3 0.321937110893046 2314 4 0.26508293044179254 2314 5 0.32035694086678035 2314 6 0.39147388969200136 2314 7 0.47657290535052965 2314 8 0.4759525052457363 2314 9 0.5105191438033714 2314 10-11 0.5921217359702879 2314 12-13 0.43672898783012215 2314 14-15 0.7422931137110069 2314 16-17 0.8348133051539932 2314 18-19 0.880010725981144 2314 20-21 0.8918637025383731 2314 22-23 0.8983946393504354 2314 24-25 0.8051446543467904 2314 26-27 0.7744513754005027 2314 28-29 0.7891276066991821 2314 30-31 0.873005966264941 2314 32-33 0.9083812288445472 2314 34-35 0.940180346079984 2314 36-37 0.8939154281882509 2314 38-39 0.6455218214432108 2314 40-41 0.6604465271485438 2314 42-43 0.8784421393653545 2314 44-45 0.966117539829149 2314 46-47 0.9034867782243623 2314 48-49 0.8860444853606211 2314 50-51 0.9016651521357275 2314 52-53 0.905860362475245 2314 54-55 0.8529184391875901 2314 56-57 0.8256174659835125 2314 58-59 0.9724403901833867 2314 60-61 1.043739162464469 2314 62-63 0.937852795205913 2314 64-65 0.9835711867407682 2314 66-67 0.685500513060326 2314 68-69 0.6824601118585889 2314 70-71 0.7831230251057733 2314 72-73 0.9822885851153487 2314 74-75 1.0596028604551364 2314 76-77 0.8953700157479503 2314 78-79 0.8330171549608423 2314 80-81 0.7951389876146777 2314 82-83 0.8226775417426779 2314 84-85 0.9108993974884925 2314 86-87 0.903039128424556 2314 88-89 0.8799829365158338 2314 90-91 0.9271195538098915 2314 92-93 0.8947096995452206 2314 94-95 0.9540009908854934 2314 96-97 0.9567720546525926 2314 98-99 1.0802258292474036 2314 100 1.0624181037449212 2315 1 0.40756278293165593 2315 2 0.4268136101995452 2315 3 0.45574553197577217 2315 4 0.30074623326785854 2315 5 0.38163577662915316 2315 6 0.4266222009633296 2315 7 0.4005339150959628 2315 8 0.2838684600412975 2315 9 0.5127506701302735 2315 10-11 0.5708857799403333 2315 12-13 0.4286752091708621 2315 14-15 0.6210438872696926 2315 16-17 0.8136983401783766 2315 18-19 0.7534136709973751 2315 20-21 0.5616482375509904 2315 22-23 0.9017448915205719 2315 24-25 0.9267764674898302 2315 26-27 0.8825809100811881 2315 28-29 0.8081947075538523 2315 30-31 0.869582580793157 2315 32-33 0.8410289354299323 2315 34-35 0.8818108139868315 2315 36-37 0.9014614334580671 2315 38-39 0.94648988514367 2315 40-41 0.9590219126973238 2315 42-43 1.0133995415364083 2315 44-45 1.0054461264427204 2315 46-47 0.9615481377527502 2315 48-49 0.8238142164069941 2315 50-51 0.9302633375105174 2315 52-53 0.9887948492817742 2315 54-55 0.752113953716723 2315 56-57 0.7490194509717938 2315 58-59 0.7564867162485669 2315 60-61 0.9227349765750006 2315 62-63 0.9401290789458372 2315 64-65 1.0225115785092598 2315 66-67 1.0546904496115772 2315 68-69 0.9853871592863896 2315 70-71 0.866711168851495 2315 72-73 0.9459150650995909 2315 74-75 0.38797213901471395 2315 76-77 0.38691729878863157 2315 78-79 0.6692469137829136 2315 80-81 0.7631586010097351 2315 82-83 0.7427520183652447 2315 84-85 0.8001445334924995 2315 86-87 0.7740581696094431 2315 88-89 0.7685820766515938 2315 90-91 0.7642968913468771 2315 92-93 0.8076301483968358 2315 94-95 0.8680347429785513 2315 96-97 1.0280488214469834 2315 98-99 1.0209624596551024 2315 100 0.9446857457967965 2316 1 0.2590497988536953 2316 2 0.05775434981266869 2316 3 0.19825894341849448 2316 4 0.02091456619579901 2316 5 0.23730644024312397 2316 6 0.32753073810219036 2316 7 0.37167426829816463 2316 8 0.389347876751728 2316 9 0.5710575081775602 2316 10-11 0.5644315165311795 2316 12-13 0.3402712547098403 2316 14-15 0.4633386085173896 2316 16-17 0.733584464592191 2316 18-19 0.7161064161404411 2316 20-21 0.7202376242215749 2316 22-23 0.7254747186489467 2316 24-25 0.4038310376540011 2316 26-27 0.6754586370823574 2316 28-29 0.8983420029705655 2316 30-31 0.5295685026191777 2316 32-33 0.6180067582011617 2316 34-35 0.7813533788837645 2316 36-37 0.8934213700054272 2316 38-39 0.7554243887965626 2316 40-41 0.7678859082013147 2316 42-43 0.7794562360928055 2316 44-45 0.5520360160057507 2316 46-47 0.7548106924135709 2316 48-49 0.8108036802940788 2316 50-51 0.8144129015726591 2316 52-53 0.8336548028814974 2316 54-55 0.7029032415445542 2316 56-57 0.8954632695111187 2316 58-59 0.7175951156080416 2316 60-61 0.8468700027293963 2316 62-63 0.9028047599909925 2316 64-65 0.8839088595592912 2316 66-67 0.8899021746054032 2316 68-69 0.8753994079076008 2316 70-71 0.8328374567804175 2316 72-73 0.8702214828188559 2316 74-75 0.9023983150359882 2316 76-77 0.7332631072480638 2316 78-79 0.510909993666111 2316 80-81 0.4835014568524798 2316 82-83 0.4061379294485121 2316 84-85 0.5926694382795219 2316 86-87 0.653194207881814 2316 88-89 0.6298131146858843 2316 90-91 0.45216045925155157 2316 92-93 0.31872172994433967 2316 94-95 0.3762588406747902 2316 96-97 0.6362620356498496 2316 98-99 0.7918563392616136 2316 100 0.2729693414872152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 11.0 12 26.0 13 131.0 14 577.0 15 1658.0 16 3889.0 17 7870.0 18 13951.0 19 20763.0 20 30226.0 21 42751.0 22 52781.0 23 55834.0 24 57380.0 25 62717.0 26 74091.0 27 92138.0 28 114306.0 29 141061.0 30 175434.0 31 219455.0 32 279294.0 33 367752.0 34 524340.0 35 733889.0 36 1156538.0 37 1729199.0 38 1478625.0 39 240132.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.023956520407616 20.618180657159037 13.77035336616935 23.587509456264005 2 15.3862606910726 19.969495296833376 40.89331443148094 23.750929580613086 3 18.923300291994842 25.348880165401216 29.442982328163854 26.284837214440092 4 12.566562085391798 16.340882834832733 38.56372616853873 32.528828911236744 5 13.164789355632761 37.977352426757385 34.7669466990949 14.090911518514954 6 31.222523720344114 37.3931413787089 17.3843330772821 14.000001823664892 7 27.85069036829542 31.95888844946808 22.086939666828716 18.10348151540778 8 26.23077515267006 36.190173859092454 19.371372290896954 18.207678697340537 9 26.050171221232215 15.815797585054991 20.98601960335246 37.14801159036033 10-11 25.73048791941352 26.36879249887421 27.19947488680536 20.701244694906915 12-13 25.666860561335813 23.702421638254293 28.023139964270495 22.6075778361394 14-15 22.92192042782178 25.260479847082944 25.603583192384527 26.214016532710747 16-17 22.03279858321646 28.123558364065982 26.67732254693037 23.16632050578719 18-19 21.614715986644782 27.671087947091834 28.88510174455051 21.829094321712876 20-21 22.757782702107512 26.82998759191353 28.659898655027515 21.752331050951444 22-23 22.87783698754528 26.68856063210309 28.27576783130683 22.157834549044797 24-25 22.403623179155257 26.59534045071419 28.92122023855271 22.079816131577843 26-27 22.383982969996367 26.890317897916894 28.231663853864188 22.49403527822255 28-29 21.91944273927903 27.128475755804395 28.669787526439112 22.282293978477465 30-31 22.62574579063822 26.82810258121736 28.578608025082758 21.967543603061667 32-33 22.70586603713029 27.011713522210318 28.05804133993486 22.22437910072454 34-35 22.673394048107188 26.986347579897753 28.49045779471089 21.84980057728417 36-37 22.5123378971337 26.710213335678755 28.267632198735427 22.509816568452116 38-39 22.523159534950715 26.87856510066563 28.630138173189668 21.968137191193986 40-41 22.384032615889875 26.28497427752295 28.96093776154463 22.370055345042545 42-43 22.359424156556013 26.389244485705614 28.479576273764078 22.771755083974295 44-45 22.401096141682892 26.976035091124757 28.353546729650738 22.269322037541613 46-47 22.417766463237278 26.841611379625164 27.878199029440857 22.862423127696697 48-49 22.8595382619109 26.846822741777842 27.904660433788226 22.388978562523032 50-51 22.61109058893028 26.776638776838364 27.851088392467073 22.76118224176429 52-53 22.312917583919337 27.110649528230972 27.669039169088283 22.90739371876141 54-55 22.247306004791785 27.069992872639105 27.69481268563414 22.987888436934973 56-57 22.846180349532975 26.804807722994497 27.386546373723995 22.962465553748526 58-59 22.185721183668015 27.11264488715397 27.889977182038038 22.81165674713997 60-61 22.3644558547467 27.471644442869653 27.46666821251135 22.697231489872298 62-63 22.540870026350362 26.996384991059685 27.284977375500773 23.177767607089176 64-65 22.054948114317316 27.035336446259283 27.505200752059146 23.404514687364255 66-67 22.315338606848574 26.885719206982422 27.45795767428241 23.34098451188659 68-69 22.10834381691265 26.884613459612456 27.597892214032893 23.409150509441996 70-71 22.024103269452542 26.61481029334631 27.688881830822005 23.672204606379147 72-73 22.321012302568715 26.763464293358975 27.68278979422088 23.23273360985144 74-75 22.45865174556905 26.966854288002118 27.384477358715532 23.1900166077133 76-77 22.25839645201104 26.859375390908408 27.237592306505547 23.644635850575 78-79 22.189336257720406 26.803681627008412 27.3134538850454 23.69352823022578 80-81 21.9120957278852 27.231025849446905 27.45791193159614 23.398966491071754 82-83 22.50562505754066 26.54104553975435 28.121150314086478 22.832179088618513 84-85 22.610634038753414 26.787816885626732 27.34266587782961 23.258883197790247 86-87 21.724083032206067 27.230656870279724 27.695321183191147 23.34993891432306 88-89 22.25895458647087 26.500036009636897 27.57718364230471 23.663825761587525 90-91 22.17308922368921 26.541652178944698 27.599696813465734 23.685561783900358 92-93 22.34300384871574 26.64570241945431 27.4745227580102 23.536770973819753 94-95 21.5807362346354 26.572300025495576 27.978360937908953 23.868602801960073 96-97 21.774198170746004 26.512765072743406 27.715554547003535 23.997482209507055 98-99 21.404684627310154 26.81254494189003 27.618388289851886 24.16438214094793 100 21.750172649684018 26.468421395530655 27.244849827350315 24.53655612743501 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 787.0 1 541.5 2 453.5 3 655.0 4 551.0 5 470.5 6 758.0 7 1516.5 8 2433.0 9 3001.0 10 2926.5 11 2805.0 12 3136.5 13 3630.0 14 4322.5 15 5145.5 16 5971.0 17 6913.5 18 7824.5 19 8655.0 20 9678.0 21 10979.0 22 12580.0 23 14778.5 24 17613.0 25 21331.0 26 26372.5 27 32656.5 28 40780.0 29 53151.5 30 69864.5 31 86594.0 32 107904.0 33 132262.5 34 152777.0 35 173589.0 36 196327.0 37 225268.0 38 240760.5 39 240171.5 40 234753.0 41 226835.0 42 228893.0 43 238450.5 44 253782.5 45 283332.0 46 309069.0 47 324463.5 48 338445.0 49 338455.0 50 327751.5 51 326140.5 52 321002.0 53 302844.5 54 285957.0 55 255390.0 56 223988.5 57 190061.0 58 157083.5 59 143950.0 60 119113.5 61 86529.0 62 65733.0 63 52216.0 64 37684.5 65 22653.0 66 14789.0 67 10518.5 68 7877.0 69 6017.5 70 4521.0 71 3305.0 72 2366.5 73 1704.0 74 1286.0 75 886.0 76 558.0 77 333.0 78 192.5 79 119.0 80 76.5 81 56.5 82 39.0 83 21.5 84 16.5 85 12.5 86 7.5 87 7.5 88 6.0 89 4.0 90 4.5 91 4.0 92 1.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043820062111420977 2 0.0 3 1.3026177797687567E-5 4 0.031718742937369225 5 0.0 6 0.0 7 0.0010030156904219426 8 0.0 9 0.010616334905115366 10-11 3.6473297833525183E-4 12-13 6.5130888988437834E-6 14-15 9.769633348265675E-5 16-17 3.061151782456578E-4 18-19 6.5130888988437834E-6 20-21 6.5130888988437834E-6 22-23 0.0 24-25 7.164397788728162E-4 26-27 5.861780008959406E-4 28-29 1.49801044673407E-4 30-31 0.002351225092482606 32-33 0.014947539022846481 34-35 0.025348941994300003 36-37 0.030272837201825907 38-39 0.02165602058865558 40-41 0.0013026177797687568 42-43 7.164397788728163E-5 44-45 0.010863832283271431 46-47 0.017930533738516937 48-49 0.003204439738231142 50-51 0.011365340128482402 52-53 0.01103317259464137 54-55 0.010368837526959303 56-57 4.4289004512137727E-4 58-59 0.00859076425757495 60-61 0.00463080620707793 62-63 0.0013612355798583507 64-65 0.03541166434301365 66-67 0.06005719273623853 68-69 0.05904766395691774 70-71 0.05196793632387455 72-73 0.04495985266871864 74-75 0.03522278476494718 76-77 0.03148427173701085 78-79 0.015195036401002547 80-81 0.0011593298239941934 82-83 0.03813413550273035 84-85 0.051459915389764736 86-87 0.05768642837705939 88-89 0.06892801981646376 90-91 0.04800146518447868 92-93 0.03895478470398467 94-95 0.013130387220069067 96-97 0.0036082512499594557 98-99 0.02162345514416136 100 0.030598491646768096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7676849.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.48030084373629 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7392158193364 49.48167722543217 2 14.322624864889752 18.47054320320138 3 4.514509616702048 8.73290814740666 4 1.8434534999284231 4.754657450672933 5 0.8814240487097034 2.8417243915852874 6 0.4893087906556155 1.8930466816175318 7 0.2942430261314957 1.3281015192291143 8 0.19054079473232086 0.9828902213875721 9 0.1284450487086706 0.7453957844361098 >10 0.55151015757827 5.971442216763459 >50 0.02693434889349791 1.196137563257948 >100 0.016149724692636033 1.9599305754431962 >500 0.001171622092449125 0.4993773878582356 >1k 3.905307908201134E-4 0.40745415107887456 >5k 2.6035385978254492E-5 0.16143047708749547 >10k+ 5.2070771956508985E-5 0.573283003542105 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18388 0.239525357343879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15761 0.20530558826935374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9616 0.12525972570256366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.6052355595375134E-5 10-11 0.0 0.0 0.0 0.0 2.6052355595375134E-5 12-13 0.0 0.0 0.0 1.3026177797687567E-5 4.5591622291906486E-5 14-15 0.0 0.0 0.0 1.3026177797687567E-5 8.467015568496919E-5 16-17 0.0 0.0 0.0 1.9539266696531352E-5 1.0420942238150053E-4 18-19 0.0 0.0 0.0 3.9078533393062704E-5 1.4328795577456323E-4 20-21 0.0 0.0 0.0 5.8617800089594056E-5 1.628272224710946E-4 22-23 0.0 0.0 0.0 2.0841884476300107E-4 1.953926669653135E-4 24-25 0.0 0.0 0.0 6.187434453901594E-4 2.4749737815606376E-4 26-27 0.0 0.0 0.0 0.0012765654241733817 2.605235559537513E-4 28-29 0.0 0.0 0.0 0.004122785272968115 2.605235559537513E-4 30-31 0.0 0.0 0.0 0.016106868846840677 2.735497337514389E-4 32-33 0.0 0.0 0.0 0.04388519300040941 2.8657591154912645E-4 34-35 0.0 0.0 0.0 0.08416864783975822 3.3868062273987676E-4 36-37 0.0 0.0 0.0 0.15105155774198503 3.517068005375643E-4 38-39 0.0 0.0 0.0 0.24745178653377187 3.517068005375643E-4 40-41 0.0 1.3026177797687567E-5 0.0 0.3495249157564516 3.517068005375643E-4 42-43 0.0 3.9078533393062704E-5 0.0 0.4494943172648049 3.582198894364081E-4 44-45 0.0 3.9078533393062704E-5 0.0 0.5684493729132878 3.7124606723409567E-4 46-47 0.0 3.9078533393062704E-5 0.0 0.7101285957298366 4.038115117283146E-4 48-49 0.0 3.9078533393062704E-5 0.0 0.8617923838283129 4.038115117283146E-4 50-51 0.0 3.9078533393062704E-5 0.0 1.017051396998951 4.038115117283146E-4 52-53 0.0 3.9078533393062704E-5 0.0 1.1666505359164938 4.233507784248459E-4 54-55 0.0 5.210471119075027E-5 0.0 1.3312037269457822 4.4940313402022105E-4 56-57 0.0 5.210471119075027E-5 0.0 1.5234505719729539 4.5591622291906483E-4 58-59 0.0 5.210471119075027E-5 0.0 1.7441205369546802 4.689424007167524E-4 60-61 0.0 5.210471119075027E-5 0.0 1.9632794653118748 4.689424007167524E-4 62-63 0.0 5.210471119075027E-5 0.0 2.186105262719118 4.949947563121275E-4 64-65 0.0 5.210471119075027E-5 0.0 2.413555353244541 5.145340230086589E-4 66-67 0.0 5.210471119075027E-5 0.0 2.66811943285585 5.275602008063464E-4 68-69 6.5130888988437834E-6 6.513088898843783E-5 0.0 2.9482278471284245 5.340732897051902E-4 70-71 1.3026177797687567E-5 6.513088898843783E-5 0.0 3.2517117374589493 5.601256453005653E-4 72-73 1.3026177797687567E-5 6.513088898843783E-5 0.0 3.552635983852229 6.382827120866908E-4 74-75 1.3026177797687567E-5 6.513088898843783E-5 0.0 3.8471252984134505 6.64335067682066E-4 76-77 1.3026177797687567E-5 6.513088898843783E-5 0.0 4.178485209231027 6.64335067682066E-4 78-79 1.3026177797687567E-5 6.513088898843783E-5 0.0 4.536861412801008 6.708481565809097E-4 80-81 1.3026177797687567E-5 6.513088898843783E-5 0.0 4.919733343719539 6.969005121762849E-4 82-83 1.3026177797687567E-5 6.513088898843783E-5 0.0 5.308955536314444 7.164397788728162E-4 84-85 1.3026177797687567E-5 6.513088898843783E-5 0.0 5.681588891483993 7.164397788728162E-4 86-87 1.3026177797687567E-5 6.513088898843783E-5 0.0 6.076002015931277 7.2295286777166E-4 88 1.3026177797687567E-5 6.513088898843783E-5 0.0 6.395514618041855 7.294659566705038E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12600 0.0 46.23355 1 GTATCAA 21715 0.0 38.1074 1 CAACGCA 24615 0.0 31.934572 5 ATCAACG 24855 0.0 31.768679 3 TATCAAC 27525 0.0 29.725737 2 AACGCAG 27795 0.0 28.974047 6 TACCTGG 4860 0.0 28.429697 2 TAGGCAT 7085 0.0 28.383694 5 TCAACGC 28375 0.0 28.092707 4 CTGTGCG 7280 0.0 27.559235 9 GGGCACC 6620 0.0 27.340794 94 GCATCGT 6325 0.0 26.668869 8 GTGGTAT 4915 0.0 26.306656 1 CTAGGCA 7945 0.0 26.088453 4 ACGCAGA 30470 0.0 25.875246 7 TAGTACT 7965 0.0 25.668892 4 GTACATG 29635 0.0 25.495728 1 CGCAGAG 30960 0.0 25.344643 8 CGACGAG 3355 0.0 24.646912 24-25 CTAGTAC 8500 0.0 24.440325 3 >>END_MODULE