FastQCFastQC Report
Mon 6 Jun 2016
ERR1211212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7957607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188810.23726982244787914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139670.17551758964723943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91810.11537388061511457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.046.691421
GTATCAA200900.040.757541
TACCTGG60600.034.8668252
CAACGCA228250.034.2067035
ATCAACG228700.034.0980723
TATCAAC247400.032.7355652
AACGCAG255950.031.1658556
TCAACGC256450.030.6833214
GTACTAG45800.030.618891
GTACATG296750.030.3838941
TACATGG303400.029.1130242
ACCTGGG75000.027.9526633
GTACCTG78700.027.7448161
GCATCGT46550.027.5670228
ACGCAGA283450.027.545527
ACATGGG313000.027.332333
CGCAGAG286100.027.1911148
CTGTGCG59350.026.0575129
CATGGGG256150.025.5076274
ATGGGGG208450.025.1434765
Produced by FastQC (version 0.11.5)