FastQCFastQC Report
Mon 6 Jun 2016
ERR1211212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7957607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177800.22343400472026326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144710.18185115198576657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.11641690774626091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118250.044.77271
GTATCAA209350.039.012421
TACCTGG54500.035.4427572
CAACGCA245300.031.9498465
GTACTAG48600.031.9266781
ATCAACG245750.031.903373
TATCAAC267700.030.2144492
AACGCAG276250.028.9485996
TCAACGC276650.028.662794
ACCTGGG65750.028.524053
GTACATG310050.027.7520711
TACATGG314600.026.9650172
GTACCTG73700.026.6676561
ACATGGG312500.026.2770673
ACGCAGA305800.025.7056587
GTGGTAT51200.025.621841
CGCAGAG309800.025.358438
TGGGGGG193650.025.2097386
TAGGCAT63650.024.9510355
ATGGGGG202450.024.4852735
Produced by FastQC (version 0.11.5)