##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7957607 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.153184242448766 33.0 31.0 34.0 30.0 34.0 2 32.29414910789136 34.0 31.0 34.0 30.0 34.0 3 32.37386251922217 34.0 31.0 34.0 30.0 34.0 4 35.98724352685424 37.0 35.0 37.0 35.0 37.0 5 35.87019250887861 37.0 35.0 37.0 35.0 37.0 6 35.89347702142114 37.0 35.0 37.0 35.0 37.0 7 35.83368655928849 37.0 35.0 37.0 35.0 37.0 8 35.81333219898897 37.0 35.0 37.0 35.0 37.0 9 37.42048331866603 39.0 37.0 39.0 34.0 39.0 10-11 37.39470358362759 39.0 37.0 39.0 34.0 39.0 12-13 37.295782325013036 39.0 37.0 39.0 34.0 39.0 14-15 38.53047499078555 40.0 38.0 41.0 34.0 41.0 16-17 38.473216885427995 40.0 38.0 41.0 34.0 41.0 18-19 38.4838872414785 40.0 38.0 41.0 34.0 41.0 20-21 38.44037271506372 40.0 38.0 41.0 34.0 41.0 22-23 38.32069308021872 40.0 38.0 41.0 34.0 41.0 24-25 38.261648508150756 40.0 38.0 41.0 34.0 41.0 26-27 38.13268951331726 40.0 37.5 41.0 33.0 41.0 28-29 38.10401085401679 40.0 37.5 41.0 33.5 41.0 30-31 38.125384364922766 40.0 37.5 41.0 33.5 41.0 32-33 38.002663551994964 40.0 37.0 41.0 33.0 41.0 34-35 37.876891759042635 40.0 37.0 41.0 33.0 41.0 36-37 37.75156551460759 40.0 37.0 41.0 33.0 41.0 38-39 37.67139505632786 40.0 37.0 41.0 33.0 41.0 40-41 37.57655762592951 40.0 37.0 41.0 32.5 41.0 42-43 37.44652406181909 40.0 36.0 41.0 32.0 41.0 44-45 37.32807513615588 40.0 36.0 41.0 32.0 41.0 46-47 37.12120264295535 39.0 36.0 41.0 31.0 41.0 48-49 36.91639729129624 39.0 35.5 41.0 31.0 41.0 50-51 36.73431441135507 39.0 35.0 41.0 30.5 41.0 52-53 36.53728099414812 39.0 35.0 40.0 30.0 41.0 54-55 36.30463724835871 38.5 35.0 40.0 30.0 41.0 56-57 36.055207363218614 38.0 35.0 40.0 29.0 41.0 58-59 35.79035575644789 38.0 34.5 40.0 29.0 41.0 60-61 35.52382581849041 37.5 34.0 40.0 28.0 41.0 62-63 35.21411028466221 37.0 34.0 40.0 28.0 41.0 64-65 34.839031319340094 36.5 34.0 39.0 28.0 41.0 66-67 34.531675100315965 36.0 33.5 39.0 27.0 40.5 68-69 34.1983987774214 35.5 33.0 39.0 26.0 40.0 70-71 33.868236393177 35.0 33.0 38.0 26.0 40.0 72-73 33.51974456642556 35.0 33.0 37.0 26.0 39.5 74-75 33.17156269466436 35.0 32.0 37.0 26.0 39.0 76-77 32.17429322659438 34.0 31.0 36.0 25.0 38.0 78-79 32.66465402224563 35.0 32.0 36.0 26.0 38.0 80-81 32.59117043603686 35.0 32.0 35.5 26.0 37.0 82-83 32.33358684338143 35.0 32.0 35.0 25.5 37.0 84-85 32.08111502616302 34.0 32.0 35.0 25.0 36.5 86-87 31.847347010225562 34.0 31.0 35.0 25.0 36.0 88-89 31.638533921064457 34.0 31.0 35.0 24.5 36.0 90-91 31.42918361009786 34.0 31.0 35.0 24.0 35.0 92-93 31.249363847699442 34.0 31.0 35.0 24.0 35.0 94-95 31.060768771315296 34.0 31.0 35.0 23.0 35.0 96-97 30.889064388326794 34.0 30.0 35.0 22.5 35.0 98-99 30.714650710948657 34.0 30.0 35.0 20.0 35.0 100 28.924665794628964 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3404116816049978 1101 2 -0.3672317305867985 1101 3 -0.37930925145850836 1101 4 -0.22938234392746892 1101 5 -0.27468471829965324 1101 6 -0.39192219935213046 1101 7 -0.3868136123698207 1101 8 -0.43925666244866335 1101 9 -0.5049625766919945 1101 10-11 -0.5038661690435049 1101 12-13 -0.46986201019938534 1101 14-15 -0.7626857760113026 1101 16-17 -0.675077372665605 1101 18-19 -0.7364593494418372 1101 20-21 -0.7123858781655059 1101 22-23 -0.7037121272037865 1101 24-25 -0.714746631487337 1101 26-27 -0.812011272597644 1101 28-29 -0.8404802222171526 1101 30-31 -0.6118902575493124 1101 32-33 -0.6845736785870642 1101 34-35 -0.6781850680340327 1101 36-37 -0.7537856414840363 1101 38-39 -0.7444546403811927 1101 40-41 -0.753770070730134 1101 42-43 -0.8094254955929685 1101 44-45 -0.8126150823215639 1101 46-47 -0.7777000009696806 1101 48-49 -0.8241156243317249 1101 50-51 -0.903660207664096 1101 52-53 -0.86675484691159 1101 54-55 -0.7956083068294291 1101 56-57 -0.8482285146551618 1101 58-59 -0.8776000589544708 1101 60-61 -0.9094547394282273 1101 62-63 -0.9599073706825223 1101 64-65 -0.9911561991423383 1101 66-67 -0.9039738919098355 1101 68-69 -1.004784842176278 1101 70-71 -1.0471128374797232 1101 72-73 -1.1167565822507086 1101 74-75 -1.2134862516146327 1101 76-77 -1.10942099672544 1101 78-79 -0.9203391544952559 1101 80-81 -0.8753260314199807 1101 82-83 -0.8587143921544538 1101 84-85 -0.8952739910546086 1101 86-87 -1.0746109979496232 1101 88-89 -1.025591982901929 1101 90-91 -1.0699348154517203 1101 92-93 -1.0051318513152943 1101 94-95 -1.0177230218666509 1101 96-97 -1.136271887301234 1101 98-99 -1.197874979451374 1101 100 -1.1596843950586972 1102 1 -0.4069613410653794 1102 2 -0.37837581063551795 1102 3 -0.4001518379409461 1102 4 -0.24909086146467274 1102 5 -0.32414367766182295 1102 6 -0.3918066993740652 1102 7 -0.3441483574259081 1102 8 -0.39344181419985347 1102 9 -0.44277468485451976 1102 10-11 -0.5266514218518097 1102 12-13 -0.5480089812978051 1102 14-15 -0.6748736356625002 1102 16-17 -0.6925359716974953 1102 18-19 -0.7004859634769858 1102 20-21 -0.7359368425747164 1102 22-23 -0.6541814216975013 1102 24-25 -0.736789044460366 1102 26-27 -0.8303966706850616 1102 28-29 -0.8502591358303278 1102 30-31 -0.7826709810262784 1102 32-33 -0.7101183977265677 1102 34-35 -0.8041890093122888 1102 36-37 -0.8584535903610657 1102 38-39 -0.7802747513793804 1102 40-41 -0.8112225902614014 1102 42-43 -0.8797256954658721 1102 44-45 -0.893907969686218 1102 46-47 -0.965943029777435 1102 48-49 -0.8894818137837959 1102 50-51 -0.9075808964567287 1102 52-53 -0.9694785591624182 1102 54-55 -0.8930636809911334 1102 56-57 -0.9956075790483752 1102 58-59 -0.9946106171662024 1102 60-61 -1.0158699058842515 1102 62-63 -1.029584756284244 1102 64-65 -0.996457913428479 1102 66-67 -1.0736067356014232 1102 68-69 -1.0379944507991112 1102 70-71 -1.1011306030188237 1102 72-73 -1.1198301671677413 1102 74-75 -1.249432649871391 1102 76-77 -1.0283503995144763 1102 78-79 -0.893190154120159 1102 80-81 -0.8808829731530032 1102 82-83 -0.8581810557416389 1102 84-85 -0.9103233690020787 1102 86-87 -0.8939959321839837 1102 88-89 -1.0506629395068465 1102 90-91 -1.0554707986496261 1102 92-93 -1.159470867139607 1102 94-95 -1.1862237082829594 1102 96-97 -1.2105864087812783 1102 98-99 -1.3425129529532462 1102 100 -1.2058909991020457 1103 1 -0.32724056339616325 1103 2 -0.37127680097108495 1103 3 -0.3987552449508627 1103 4 -0.2463242266082446 1103 5 -0.2564275014886732 1103 6 -0.3164287189934427 1103 7 -0.33744953608036354 1103 8 -0.3923211549276857 1103 9 -0.5790251534051833 1103 10-11 -0.5472522699001345 1103 12-13 -0.6129707501057098 1103 14-15 -0.7039433120413818 1103 16-17 -0.7339934067428757 1103 18-19 -0.706662438043999 1103 20-21 -0.7071544248996489 1103 22-23 -0.7694634702284944 1103 24-25 -0.8775845200091368 1103 26-27 -0.8343953708445255 1103 28-29 -0.9113402774036601 1103 30-31 -0.911550649955494 1103 32-33 -0.7797239736022945 1103 34-35 -0.9245454278708038 1103 36-37 -0.9283702588440619 1103 38-39 -0.8198215632910504 1103 40-41 -0.9480350090759586 1103 42-43 -0.9230049587684519 1103 44-45 -0.9223448248740809 1103 46-47 -0.9379730997790432 1103 48-49 -1.0276272717472992 1103 50-51 -1.0645715288204514 1103 52-53 -1.0607988493079787 1103 54-55 -1.0525647822707995 1103 56-57 -1.0106772341164785 1103 58-59 -0.9536757035777867 1103 60-61 -1.0628333219875472 1103 62-63 -0.9950224811572639 1103 64-65 -1.077026968447008 1103 66-67 -1.279449446239802 1103 68-69 -1.1599149013421837 1103 70-71 -1.2477957983754138 1103 72-73 -1.1379812082425431 1103 74-75 -1.020293666907918 1103 76-77 -1.0332796893885643 1103 78-79 -0.9284519873087262 1103 80-81 -0.8892771555611247 1103 82-83 -0.9436550426954469 1103 84-85 -0.9926831206970554 1103 86-87 -0.9602959416767867 1103 88-89 -1.133172672056098 1103 90-91 -1.1047822618275163 1103 92-93 -1.1454680412693499 1103 94-95 -1.0945624652202923 1103 96-97 -1.1877324416891852 1103 98-99 -1.2974492813982543 1103 100 -1.4074015096108674 1104 1 -0.4233439944039965 1104 2 -0.359716819828666 1104 3 -0.4256269663573313 1104 4 -0.2923859473492527 1104 5 -0.34019096585379316 1104 6 -0.4005680750505789 1104 7 -0.34655117424495074 1104 8 -0.39897636677628867 1104 9 -0.564581008535086 1104 10-11 -0.5761512670828068 1104 12-13 -0.5705210066279491 1104 14-15 -0.760236988752446 1104 16-17 -0.719640343396243 1104 18-19 -0.760950907232349 1104 20-21 -0.757212693670418 1104 22-23 -0.7735599836433735 1104 24-25 -0.796296444714244 1104 26-27 -0.8424963702292203 1104 28-29 -0.9162671539580387 1104 30-31 -0.8953514889509009 1104 32-33 -0.8317179587891204 1104 34-35 -0.7938352599664071 1104 36-37 -0.792719084071841 1104 38-39 -0.7748583793399959 1104 40-41 -0.8242584270210997 1104 42-43 -0.7107732582167259 1104 44-45 -0.8968302864295978 1104 46-47 -0.9481130478075741 1104 48-49 -0.9218318792319664 1104 50-51 -0.9330882894200627 1104 52-53 -0.8729402328749387 1104 54-55 -0.9679926524524447 1104 56-57 -0.93385826783053 1104 58-59 -0.9966263466757539 1104 60-61 -0.992630696998539 1104 62-63 -0.9878177961265351 1104 64-65 -0.9752212587159832 1104 66-67 -1.0456447357920595 1104 68-69 -1.026686134278627 1104 70-71 -1.1965827251069427 1104 72-73 -1.0536668274334602 1104 74-75 -0.9931224296650853 1104 76-77 -0.9541691841353632 1104 78-79 -0.7328245460020888 1104 80-81 -0.706117614356117 1104 82-83 -0.7404322799911753 1104 84-85 -0.7409287969381673 1104 86-87 -0.8525397121881717 1104 88-89 -0.9325634322314613 1104 90-91 -0.9392440796337311 1104 92-93 -0.9954077012228204 1104 94-95 -0.9583343172770604 1104 96-97 -0.958275567249764 1104 98-99 -1.0427943719325121 1104 100 -1.1188365978959744 1105 1 -0.44036710874796725 1105 2 -0.423532059316468 1105 3 -0.46839312989447635 1105 4 -0.26291562150270664 1105 5 -0.27899579416971676 1105 6 -0.3634940462201186 1105 7 -0.42819427310326574 1105 8 -0.5135577142749881 1105 9 -0.6158313517181639 1105 10-11 -0.5907051213757626 1105 12-13 -0.6555195490638894 1105 14-15 -0.8212360448061915 1105 16-17 -0.7622293242119227 1105 18-19 -0.7684535832891228 1105 20-21 -0.904771775142855 1105 22-23 -0.8652080644402389 1105 24-25 -0.8939545865591327 1105 26-27 -0.9268273251881851 1105 28-29 -0.8902684526553273 1105 30-31 -0.8715996727798085 1105 32-33 -0.8555082577362825 1105 34-35 -0.8934318848958398 1105 36-37 -0.8098064006424224 1105 38-39 -0.8955585653529283 1105 40-41 -0.9408716111122075 1105 42-43 -0.8836991985383449 1105 44-45 -0.938056136366832 1105 46-47 -0.9013183005908232 1105 48-49 -0.9097659865395329 1105 50-51 -0.9517358550567181 1105 52-53 -1.0182971201350526 1105 54-55 -0.955788869572423 1105 56-57 -1.0545411018903295 1105 58-59 -1.1806975621762064 1105 60-61 -0.9733932420750548 1105 62-63 -1.0227160057134554 1105 64-65 -1.0471651556098962 1105 66-67 -0.9865116702186398 1105 68-69 -0.9432924932681814 1105 70-71 -0.978016636530235 1105 72-73 -0.9894839193386602 1105 74-75 -0.9946160086712226 1105 76-77 -0.9487482026163505 1105 78-79 -0.925158298867089 1105 80-81 -0.8445285147759591 1105 82-83 -0.7972522530917914 1105 84-85 -0.8978204143252846 1105 86-87 -0.8700503392954708 1105 88-89 -0.8619875633227707 1105 90-91 -0.8761617095265954 1105 92-93 -0.9960367288039471 1105 94-95 -0.9310089627573781 1105 96-97 -1.0018147024592636 1105 98-99 -1.0846757015394175 1105 100 -1.2510842388015462 1106 1 -0.48088564606289097 1106 2 -0.43359234451775563 1106 3 -0.477535488322296 1106 4 -0.31284401360257874 1106 5 -0.31659811176660924 1106 6 -0.34553065179419207 1106 7 -0.40548245789586446 1106 8 -0.39659558091704383 1106 9 -0.5063861255140907 1106 10-11 -0.5394067115517345 1106 12-13 -0.5696945011010115 1106 14-15 -0.6838530684550648 1106 16-17 -0.7805763735819724 1106 18-19 -0.7380737634526824 1106 20-21 -0.7271223789560466 1106 22-23 -0.6903945333345334 1106 24-25 -0.7320881963278083 1106 26-27 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2311 34-35 1.035045788102913 2311 36-37 1.0158533046428246 2311 38-39 1.048129017046172 2311 40-41 1.0069647495372749 2311 42-43 1.0105834101185636 2311 44-45 1.0042131862419765 2311 46-47 0.6545495554549987 2311 48-49 0.8816604737998759 2311 50-51 0.9973118652767852 2311 52-53 0.9008801706566274 2311 54-55 0.9459479431544295 2311 56-57 1.0408131184589138 2311 58-59 1.0710352772724576 2311 60-61 1.1127846217598787 2311 62-63 1.1234843743547458 2311 64-65 1.1707252609322438 2311 66-67 1.2270507546604037 2311 68-69 1.231145588649973 2311 70-71 1.2171982665752523 2311 72-73 1.2618076485778076 2311 74-75 1.2513324804136232 2311 76-77 1.1839784940699758 2311 78-79 0.8767794132019944 2311 80-81 0.8805277349603884 2311 82-83 0.9904979668867142 2311 84-85 0.9674524288307857 2311 86-87 0.9832982281557996 2311 88-89 1.064683634613921 2311 90-91 1.1431104572067134 2311 92-93 1.2082663908804 2311 94-95 1.2303290576083867 2311 96-97 1.2345375697211338 2311 98-99 1.2797007403520944 2311 100 1.435418408961329 2312 1 0.262574119832486 2312 2 0.3657762551429613 2312 3 0.42745215034978656 2312 4 0.15585037948356018 2312 5 0.34083834185823036 2312 6 0.3946193950512793 2312 7 0.33073874348313126 2312 8 0.44809868124724517 2312 9 0.6226900907107691 2312 10-11 0.6574320094757695 2312 12-13 0.6833978393389231 2312 14-15 0.8803003451645992 2312 16-17 0.8543185746574196 2312 18-19 0.8378384400623489 2312 20-21 0.885215926059864 2312 22-23 0.9656417632584819 2312 24-25 0.9033561773246248 2312 26-27 0.9826366214707676 2312 28-29 0.9875919404927771 2312 30-31 0.9972625925021461 2312 32-33 0.8470786237434709 2312 34-35 0.9297036218648032 2312 36-37 1.0156997620442425 2312 38-39 0.8670840962662538 2312 40-41 0.969541284464654 2312 42-43 1.044323898986839 2312 44-45 0.8008355249600285 2312 46-47 0.7853008146635219 2312 48-49 0.9900437364496284 2312 50-51 0.8353238317595029 2312 52-53 0.9918153924516488 2312 54-55 0.8611891180903939 2312 56-57 0.9295106209748027 2312 58-59 1.0106428590834327 2312 60-61 1.108414577774873 2312 62-63 1.0575066645281836 2312 64-65 1.1030945512859844 2312 66-67 1.0754154317240605 2312 68-69 1.1100026394791058 2312 70-71 1.0587358387319128 2312 72-73 1.0617368433344154 2312 74-75 1.0974069736911432 2312 76-77 1.09593090655504 2312 78-79 1.0285385749056175 2312 80-81 0.9649885101257851 2312 82-83 0.9410902071401281 2312 84-85 1.0660923232718282 2312 86-87 1.0659154115808 2312 88-89 1.102549674128177 2312 90-91 1.165071152623522 2312 92-93 1.1975180296346828 2312 94-95 1.2294539843474581 2312 96-97 0.8856851129849517 2312 98-99 0.6983497804464633 2312 100 0.7727817766538934 2313 1 0.07190747084675309 2313 2 0.24567299251686592 2313 3 0.29039344501642717 2313 4 0.20579003207125623 2313 5 0.32146155194499215 2313 6 0.23836542527038773 2313 7 0.23898414415623392 2313 8 0.3835477738783908 2313 9 0.5861548570370516 2313 10-11 0.37345181444011644 2313 12-13 0.42283306397738585 2313 14-15 0.5025852173157475 2313 16-17 0.7330285413977933 2313 18-19 0.6925594900488861 2313 20-21 0.668009799977149 2313 22-23 0.632011673117205 2313 24-25 0.5656376005705823 2313 26-27 0.7518003692855615 2313 28-29 0.5924606618333783 2313 30-31 0.8424609613014766 2313 32-33 0.8886578784383232 2313 34-35 0.9468125402908356 2313 36-37 0.9838205503679944 2313 38-39 0.9761469627616037 2313 40-41 0.9455196991954438 2313 42-43 1.0445579840144248 2313 44-45 0.8525394009945302 2313 46-47 0.884810241895778 2313 48-49 0.9298601443379013 2313 50-51 0.8891284326821776 2313 52-53 0.9399978396482211 2313 54-55 0.9280838425353508 2313 56-57 0.6229837111983443 2313 58-59 0.7529167383409572 2313 60-61 0.5815546806419292 2313 62-63 0.9075265724315145 2313 64-65 1.0278860155771739 2313 66-67 0.9958675518399147 2313 68-69 1.1348907495330067 2313 70-71 1.0730774743108569 2313 72-73 1.0317137303878852 2313 74-75 1.1146775152984958 2313 76-77 1.0697065901664757 2313 78-79 0.968589348768468 2313 80-81 0.8344615019055013 2313 82-83 0.8562734475062328 2313 84-85 0.8912391742606935 2313 86-87 0.9229125479443745 2313 88-89 0.9396919668084109 2313 90-91 0.8197805489291419 2313 92-93 1.012738287575484 2313 94-95 1.167655997815828 2313 96-97 1.1248487967438336 2313 98-99 1.1451946221923954 2313 100 1.2766383471497846 2314 1 -0.3893760498603491 2314 2 0.02883300167399483 2314 3 0.30153001601058094 2314 4 0.23814910028639247 2314 5 0.33702670922633615 2314 6 0.4019514743945294 2314 7 0.4781612512392499 2314 8 0.4696327174536705 2314 9 0.4753180492261535 2314 10-11 0.5990539608300764 2314 12-13 0.4787550372639586 2314 14-15 0.7995728448316015 2314 16-17 0.8502878436009951 2314 18-19 0.8539495181123442 2314 20-21 0.8802348380718072 2314 22-23 0.9509964177727497 2314 24-25 0.8684846480391712 2314 26-27 0.770502603820745 2314 28-29 0.856165778174713 2314 30-31 0.9316968069817904 2314 32-33 0.9155365955553734 2314 34-35 0.939920429209927 2314 36-37 0.9245904955324207 2314 38-39 0.6569977364173951 2314 40-41 0.7103783066554072 2314 42-43 0.8873406342500445 2314 44-45 0.943029146402921 2314 46-47 0.9445340712218737 2314 48-49 1.0003319334717347 2314 50-51 1.0123228236622381 2314 52-53 1.0134995881459972 2314 54-55 0.9367775107139664 2314 56-57 0.9534250518386358 2314 58-59 1.0158317408278918 2314 60-61 1.0628372486531745 2314 62-63 0.9792518440223077 2314 64-65 0.9971451138006913 2314 66-67 0.7120122152584827 2314 68-69 0.6635846729058628 2314 70-71 0.8876414620032023 2314 72-73 1.0005370657879595 2314 74-75 1.0453829468332643 2314 76-77 0.8790736087581479 2314 78-79 0.7619155209972561 2314 80-81 0.8044472448492712 2314 82-83 0.8533172133614357 2314 84-85 0.8691793127209131 2314 86-87 0.9717137126315372 2314 88-89 0.9491309470996825 2314 90-91 1.0060812897566827 2314 92-93 0.9491261172789685 2314 94-95 0.9326501964295097 2314 96-97 0.9077689189278466 2314 98-99 1.0628354705230372 2314 100 1.0413646357401163 2315 1 0.44494459998349356 2315 2 0.4546828707345938 2315 3 0.4573923793542889 2315 4 0.33571210260540596 2315 5 0.3947459276653973 2315 6 0.45393516014821955 2315 7 0.4257335918937528 2315 8 0.3004336376615555 2315 9 0.5303450155735874 2315 10-11 0.5987815077978098 2315 12-13 0.44932818689025567 2315 14-15 0.6634788710458608 2315 16-17 0.8165980973124931 2315 18-19 0.7797669336357984 2315 20-21 0.5385929573102217 2315 22-23 0.8647324069551416 2315 24-25 0.908733755999684 2315 26-27 0.8600121820078144 2315 28-29 0.8129612124857672 2315 30-31 0.8551346342768653 2315 32-33 0.8211564886839753 2315 34-35 0.888795589831382 2315 36-37 0.9010301424388061 2315 38-39 0.9503558592715038 2315 40-41 0.9283101799097295 2315 42-43 0.9989233345294011 2315 44-45 0.9906476451469999 2315 46-47 0.9281642528887133 2315 48-49 0.8894371232744192 2315 50-51 0.9314351652953903 2315 52-53 0.9547823412648029 2315 54-55 0.7717354310106614 2315 56-57 0.7433221506786793 2315 58-59 0.7731167107796679 2315 60-61 0.8712077455961449 2315 62-63 0.9245085886441515 2315 64-65 1.016169361939781 2315 66-67 0.9865472098681494 2315 68-69 1.0383483959256878 2315 70-71 0.9792909466657989 2315 72-73 1.013043959973274 2315 74-75 0.3752072368967987 2315 76-77 0.4055484308285031 2315 78-79 0.7300586764202635 2315 80-81 0.7596966165358623 2315 82-83 0.6957641763162599 2315 84-85 0.7713022769599469 2315 86-87 0.814536329113718 2315 88-89 0.8336879413069767 2315 90-91 0.9047308051635774 2315 92-93 0.8937594241929396 2315 94-95 1.0095534873649967 2315 96-97 1.2060848126302162 2315 98-99 1.14265059536584 2315 100 1.0913317537987446 2316 1 0.25755652993444045 2316 2 0.050328292468755365 2316 3 0.20034543755119216 2316 4 0.0015599122492204742 2316 5 0.22979930187537434 2316 6 0.35215057530124483 2316 7 0.39931972523043413 2316 8 0.43109778475455585 2316 9 0.5987143482450676 2316 10-11 0.6081692520155428 2316 12-13 0.3662415762950886 2316 14-15 0.5270577410041781 2316 16-17 0.8048648172003325 2316 18-19 0.767026549084143 2316 20-21 0.7478915154779173 2316 22-23 0.7471733046270472 2316 24-25 0.4481155843066418 2316 26-27 0.7191960076952171 2316 28-29 0.9531262654463859 2316 30-31 0.5532058865251841 2316 32-33 0.6598715823359598 2316 34-35 0.8236414277405899 2316 36-37 0.8551842089541779 2316 38-39 0.7340493649416473 2316 40-41 0.7334200359668799 2316 42-43 0.7469066512058902 2316 44-45 0.48743392167447297 2316 46-47 0.7578760912321911 2316 48-49 0.8840069658405341 2316 50-51 0.8839184128641762 2316 52-53 0.8944144839366786 2316 54-55 0.7358199846354623 2316 56-57 0.9342602311513204 2316 58-59 0.7660472725201117 2316 60-61 0.8708007657537635 2316 62-63 0.8817752136193775 2316 64-65 0.9800594507378761 2316 66-67 0.9389744896998096 2316 68-69 1.0186508896973763 2316 70-71 0.9449587250061171 2316 72-73 0.9653933886991553 2316 74-75 0.951866720899126 2316 76-77 0.8381063962377198 2316 78-79 0.5797093511350013 2316 80-81 0.5619795467748148 2316 82-83 0.47833816706766896 2316 84-85 0.6352353183588981 2316 86-87 0.6908971023723289 2316 88-89 0.6505330327292995 2316 90-91 0.5331245499976234 2316 92-93 0.34582143072353233 2316 94-95 0.381804108870341 2316 96-97 0.6098988076199987 2316 98-99 0.8415932829006714 2316 100 0.34594795154786695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 2.0 12 29.0 13 180.0 14 625.0 15 1785.0 16 3964.0 17 8197.0 18 13972.0 19 20269.0 20 29188.0 21 41110.0 22 51322.0 23 56104.0 24 58903.0 25 65579.0 26 78083.0 27 96708.0 28 120221.0 29 149335.0 30 184061.0 31 231390.0 32 294347.0 33 387513.0 34 555940.0 35 764013.0 36 1182155.0 37 1803637.0 38 1536056.0 39 222896.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34062427710783 20.101380912629367 13.736635923843624 23.82135888641918 2 15.679977661626165 20.073974500122965 40.75471432555038 23.49133351270049 3 19.13615487671105 25.52717167359484 29.308082191040597 26.02859125865351 4 13.01268519673193 16.2411826644193 37.74231959032451 33.00381254852426 5 13.440774343342163 37.74127071115726 34.05021132609338 14.767743619407192 6 31.301570937092016 36.34115130340063 18.094723702741287 14.26255405676606 7 28.20805173458129 31.983427039009545 21.819318533114505 17.98920269329466 8 26.613000616894 35.91288185003356 19.523645739227884 17.950471793844557 9 27.008351399754925 16.1406227416973 20.50467841769567 36.34634744085211 10-11 25.99616679246938 26.117120870698457 27.23957471666429 20.647137620167875 12-13 25.815863990267424 23.414275170915076 27.82276128992045 22.94709954889705 14-15 23.186525176699092 24.962457254678423 25.68063897689704 26.17037859172544 16-17 22.539940833793377 27.740376694782825 26.522400080526964 23.197282390896834 18-19 22.18910785616832 27.42201267290531 28.453359156842 21.93552031408437 20-21 23.24102585111328 26.68378194600462 28.083078241989078 21.99211396089302 22-23 23.190420185364772 26.336567010660367 27.935169454837368 22.5378433491375 24-25 22.59535707158623 26.111433096862307 28.5008389502114 22.79237088134007 26-27 22.598533596646714 26.50320795368935 27.9124015952116 22.985856854452337 28-29 22.31567592442666 27.304206063779702 27.95778297072061 22.422335041073026 30-31 23.40469903738864 26.57773244760203 27.993317429512615 22.02425108549671 32-33 23.330559660065507 26.54607042809105 27.649515202090303 22.473854709753137 34-35 23.086830090425334 26.818066060847862 28.022752521081607 22.072351327645205 36-37 22.884371430940085 26.650841156575446 27.868671731307575 22.596115681176897 38-39 22.973110557468836 26.787535733368827 27.98831393301917 22.251039776143163 40-41 22.92925934178096 26.131890870792006 28.333881872240575 22.60496791518646 42-43 22.807516459888515 26.33942823859322 27.77292532518431 23.08012997633395 44-45 22.50163933293053 26.844402718826206 28.054301116317387 22.59965683192588 46-47 22.496101624412717 26.496297195199443 27.60656989641473 23.40103128397312 48-49 22.9262548950822 26.618012268785264 27.594769790464753 22.860963045667788 50-51 22.88330790626265 26.55028571363936 27.61074474819659 22.955661631901396 52-53 22.572449248968756 26.90094503924619 27.362326333980562 23.164279377804494 54-55 22.419699249339292 26.63188756823496 27.640177280022645 23.30823590240311 56-57 23.01465100955071 26.510804265797322 27.17069127031107 23.303853454340892 58-59 22.360779453706765 26.911670974573514 27.745622169391638 22.981927402328086 60-61 22.86949589008111 27.088824944110456 27.299991843878 22.741687321930428 62-63 22.989999313226818 26.745756379497664 27.08667360309675 23.177570704178766 64-65 22.450451037095874 26.663606472718755 27.25630272462648 23.629639765558892 66-67 22.55995231917484 26.550838642467056 27.2150317825015 23.674177255856602 68-69 22.10931381991803 26.597211150168565 27.559274815552858 23.73420021436055 70-71 22.254335205608136 26.33847259114539 27.58555006273641 23.82164214051007 72-73 22.467239157918875 26.741593672020446 27.32312439213036 23.46804277793032 74-75 22.651455691711188 26.817643351492332 27.071369815545314 23.459531141251162 76-77 22.441052349801176 26.518892430488727 26.98552839579439 24.05452682391571 78-79 22.29716456398128 26.564967152911745 27.20508601049242 23.932782272614556 80-81 22.17668176853179 26.961849652580028 27.19550925188212 23.665959327006068 82-83 22.762501588701596 26.51826758549666 27.661994448752917 23.057236377048827 84-85 22.87151779421591 26.82849259740225 26.87207075654689 23.42791885183495 86-87 22.02653552075546 27.22026214890076 27.17470115806143 23.578501172282344 88-89 22.285413196664773 26.440895689621023 27.305335644676838 23.968355469037366 90-91 22.551947250644993 26.271003337978723 27.348524419031463 23.82852499234482 92-93 22.621729678343073 26.520086476499998 27.133805877430106 23.72437796772682 94-95 22.001767965883424 26.487043494637753 27.397268796231657 24.113919743247163 96-97 21.9321882177564 26.447511214381702 27.285194377065753 24.33510619079614 98-99 21.624306890881616 26.679588447096204 27.19702738297935 24.499077279042833 100 21.771365545510726 26.538654160324747 27.05042008778374 24.63956020638079 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1007.0 1 723.0 2 788.0 3 1151.5 4 897.0 5 661.0 6 941.5 7 1797.5 8 2918.5 9 3473.0 10 3244.5 11 3032.0 12 3326.5 13 3919.5 14 4617.5 15 5319.5 16 6079.5 17 6972.5 18 7796.5 19 8506.5 20 9221.5 21 10149.0 22 11627.0 23 13763.0 24 16257.0 25 19409.0 26 24068.0 27 29913.0 28 37596.5 29 49400.0 30 64754.0 31 80427.0 32 100539.5 33 121782.0 34 140591.5 35 160711.5 36 181531.5 37 208414.5 38 225059.0 39 227386.0 40 226731.0 41 225522.5 42 231950.5 43 245286.0 44 263568.5 45 293598.5 46 320307.5 47 338875.0 48 356028.5 49 363529.5 50 364591.0 51 369526.0 52 364267.5 53 343234.0 54 320759.0 55 284572.0 56 248381.5 57 212303.5 58 174869.0 59 150682.5 60 120720.0 61 89419.0 62 68619.0 63 54038.5 64 39655.5 65 25057.5 66 16639.5 67 12114.0 68 8979.0 69 6564.0 70 4932.5 71 3730.5 72 2769.0 73 2059.5 74 1538.5 75 1075.0 76 713.0 77 432.0 78 246.0 79 146.5 80 96.0 81 73.0 82 48.5 83 26.0 84 20.0 85 15.5 86 5.5 87 5.0 88 4.5 89 2.5 90 1.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04331704242242674 2 0.0 3 0.0 4 0.03547548905091694 5 0.0 6 0.0 7 8.419616600819819E-4 8 0.0 9 0.010769569293884456 10-11 5.21513565573168E-4 12-13 0.0 14-15 1.382325113567433E-4 16-17 3.015982065965308E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.403634534854511E-4 26-27 5.277968615439289E-4 28-29 1.570823992690265E-4 30-31 0.0023876524688892023 32-33 0.01571452322287341 34-35 0.02630187693360579 36-37 0.03164896180472346 38-39 0.02272039823027199 40-41 0.0013069255619183003 42-43 5.6549663736849537E-5 44-45 0.01086381873344587 46-47 0.017700044749633905 48-49 0.0032484640168834672 50-51 0.01085753543747511 52-53 0.010650186670439996 54-55 0.010260622320252811 56-57 4.084142380994688E-4 58-59 0.009129629045515819 60-61 0.004951237224959715 62-63 0.0013006422659475392 64-65 0.03485344274981159 66-67 0.05937086362772125 68-69 0.05844721912001938 70-71 0.05141621092873775 72-73 0.0452271643975381 74-75 0.03494140889340225 76-77 0.031121164943179527 78-79 0.015004510778177408 80-81 0.0011435598666785127 82-83 0.037674642640683306 84-85 0.05129054500932253 86-87 0.05694551138300748 88-89 0.06939272070108514 90-91 0.04827456294335722 92-93 0.038654836812122034 94-95 0.012635708197200491 96-97 0.0038013940623104407 98-99 0.02248163298338307 100 0.032710838823782076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7957607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.62348530899911 #Duplication Level Percentage of deduplicated Percentage of total 1 77.13402174972246 49.07535299617485 2 14.114768786657722 17.960615690756732 3 4.320038005418994 8.245676237162797 4 1.7382667126675022 4.423783466260919 5 0.8460175639970778 2.691329302706164 6 0.4912545266423847 1.8753195095286643 7 0.302486716168708 1.3471681399629023 8 0.20161436462602061 1.0261926852693448 9 0.14616690991533438 0.8369683421094065 >10 0.6533184304978278 7.18003113621387 >50 0.031975403442783504 1.404257965120899 >100 0.018272516832370186 2.1821998717106204 >500 0.0012317415498011269 0.5387093692392355 >1k 4.926711837583085E-4 0.5617419489494888 >5k 2.463355911574351E-5 0.14519216020618156 >10k+ 4.926711823148702E-5 0.5054611786279752 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17780 0.22343400472026326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14471 0.18185115198576657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.11641690774626091 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2566591941522118E-5 0.0 8 0.0 0.0 0.0 1.2566591941522118E-5 0.0 9 0.0 0.0 0.0 1.2566591941522118E-5 0.0 10-11 1.8849887912283177E-5 0.0 0.0 2.5133183883044237E-5 0.0 12-13 2.5133183883044237E-5 0.0 0.0 2.5133183883044237E-5 0.0 14-15 2.5133183883044237E-5 0.0 0.0 3.769977582456635E-5 0.0 16-17 2.5133183883044237E-5 0.0 0.0 5.654966373684953E-5 1.2566591941522118E-5 18-19 2.5133183883044237E-5 0.0 0.0 7.53995516491327E-5 2.5133183883044237E-5 20-21 2.5133183883044237E-5 0.0 0.0 1.0053273553217695E-4 2.5133183883044237E-5 22-23 2.5133183883044237E-5 0.0 0.0 2.764650227134866E-4 6.283295970761059E-5 24-25 2.5133183883044237E-5 0.0 0.0 7.979785882866545E-4 1.0681603150293801E-4 26-27 2.5133183883044237E-5 0.0 0.0 0.001696489912105486 1.2566591941522119E-4 28-29 2.5133183883044237E-5 0.0 0.0 0.004907254153164387 1.2566591941522119E-4 30-31 3.769977582456635E-5 0.0 0.0 0.01742986302289118 1.2566591941522119E-4 32-33 5.654966373684953E-5 0.0 0.0 0.04914165778732224 1.570823992690265E-4 34-35 7.53995516491327E-5 0.0 0.0 0.09457617095189547 1.8849887912283177E-4 36-37 7.53995516491327E-5 0.0 0.0 0.16555228223761237 1.8849887912283177E-4 38-39 8.796614359065483E-5 0.0 0.0 0.27512039737574373 1.8849887912283177E-4 40-41 8.796614359065483E-5 0.0 0.0 0.40282838798146225 1.8849887912283177E-4 42-43 8.796614359065483E-5 0.0 0.0 0.5231974888933318 1.8849887912283177E-4 44-45 8.796614359065483E-5 0.0 0.0 0.6605063557423734 2.1363206300587602E-4 46-47 8.796614359065483E-5 0.0 0.0 0.8128888496252704 2.890316146550087E-4 48-49 8.796614359065483E-5 0.0 0.0 0.9825566907237313 3.392979824210972E-4 50-51 8.796614359065483E-5 0.0 0.0 1.1661545989893696 3.392979824210972E-4 52-53 8.796614359065483E-5 0.0 0.0 1.3471512227231126 3.4558127839185826E-4 54-55 8.796614359065483E-5 0.0 0.0 1.542209862839419 3.5186457436261933E-4 56-57 8.796614359065483E-5 0.0 0.0 1.7629043505164304 3.5186457436261933E-4 58-59 8.796614359065483E-5 0.0 0.0 2.015511698428937 3.5186457436261933E-4 60-61 8.796614359065483E-5 0.0 0.0 2.269463671679187 3.5186457436261933E-4 62-63 8.796614359065483E-5 0.0 0.0 2.543076078021948 3.5186457436261933E-4 64-65 1.0053273553217695E-4 0.0 0.0 2.8029029330048596 3.5186457436261933E-4 66-67 1.1309932747369906E-4 0.0 0.0 3.0971622499075413 3.5186457436261933E-4 68-69 1.1309932747369906E-4 0.0 0.0 3.420243045428104 3.5186457436261933E-4 70-71 1.1309932747369906E-4 0.0 0.0 3.7570654092367217 3.581478703333804E-4 72-73 1.2566591941522119E-4 0.0 0.0 4.08010850498146 4.146975340702299E-4 74-75 1.2566591941522119E-4 0.0 0.0 4.404810390862479 4.146975340702299E-4 76-77 1.2566591941522119E-4 0.0 0.0 4.773834646521246 4.146975340702299E-4 78-79 1.2566591941522119E-4 0.0 0.0 5.178183592127633 4.3983071795327414E-4 80-81 1.2566591941522119E-4 0.0 0.0 5.587502624846891 4.712471978070794E-4 82-83 1.3194921538598224E-4 0.0 0.0 5.988508856996834 4.900970857193626E-4 84-85 1.382325113567433E-4 0.0 0.0 6.395873784669185 4.900970857193626E-4 86-87 1.382325113567433E-4 0.0 0.0 6.83124964577919 4.900970857193626E-4 88 1.382325113567433E-4 0.0 0.0 7.175900996367376 5.026636776608847E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11825 0.0 44.7727 1 GTATCAA 20935 0.0 39.01242 1 TACCTGG 5450 0.0 35.442757 2 CAACGCA 24530 0.0 31.949846 5 GTACTAG 4860 0.0 31.926678 1 ATCAACG 24575 0.0 31.90337 3 TATCAAC 26770 0.0 30.214449 2 AACGCAG 27625 0.0 28.948599 6 TCAACGC 27665 0.0 28.66279 4 ACCTGGG 6575 0.0 28.52405 3 GTACATG 31005 0.0 27.752071 1 TACATGG 31460 0.0 26.965017 2 GTACCTG 7370 0.0 26.667656 1 ACATGGG 31250 0.0 26.277067 3 ACGCAGA 30580 0.0 25.705658 7 GTGGTAT 5120 0.0 25.62184 1 CGCAGAG 30980 0.0 25.35843 8 TGGGGGG 19365 0.0 25.209738 6 TAGGCAT 6365 0.0 24.951035 5 ATGGGGG 20245 0.0 24.485273 5 >>END_MODULE