FastQCFastQC Report
Mon 6 Jun 2016
ERR1211211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8666215
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264940.30571593250340545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211640.2442127272402081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131210.15140404432615623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143900.045.8514331
GTATCAA244500.039.0543021
TACCTGG56450.033.0968932
CAACGCA282150.032.272765
ATCAACG288100.031.6388783
GTACTAG57200.031.3469051
TATCAAC309350.030.5930392
AACGCAG314900.029.6032316
TCAACGC320000.028.7197594
GTACATG319200.027.8948441
GCATCGT56900.027.8409548
ACGCAGA340900.026.7664097
TACATGG324300.026.6432022
GTGGTAT56900.026.2187941
CGCAGAG348900.026.0838018
ACATGGG323400.025.8887123
TAGGCAT73400.025.8744935
CTAACGC26200.025.1196333
TCTAACG26650.025.0756282
ACCTGGG73300.025.0119293
Produced by FastQC (version 0.11.5)