##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8666215 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42970777900156 31.0 31.0 34.0 28.0 34.0 2 31.445093042348937 31.0 31.0 34.0 28.0 34.0 3 31.509346929426513 33.0 31.0 34.0 28.0 34.0 4 35.10470534137452 37.0 35.0 37.0 32.0 37.0 5 35.16079591840267 37.0 35.0 37.0 32.0 37.0 6 35.223342254952136 37.0 35.0 37.0 32.0 37.0 7 35.13955019578905 37.0 35.0 37.0 32.0 37.0 8 35.14074771973693 37.0 35.0 37.0 32.0 37.0 9 36.710501297279144 39.0 37.0 39.0 32.0 39.0 10-11 36.65903949994317 39.0 37.0 39.0 32.0 39.0 12-13 36.5515907463639 39.0 37.0 39.0 32.0 39.0 14-15 37.7482258979266 40.0 37.0 41.0 32.0 41.0 16-17 37.721333938749495 40.0 37.0 41.0 32.0 41.0 18-19 37.733628060231595 40.0 37.0 41.0 32.0 41.0 20-21 37.700933510188705 40.0 37.0 41.0 32.0 41.0 22-23 37.59368599786643 40.0 37.0 41.0 32.0 41.0 24-25 37.5007573086982 40.0 37.0 41.0 31.5 41.0 26-27 37.35236767146903 40.0 37.0 41.0 31.0 41.0 28-29 37.23664812146941 40.0 36.0 41.0 31.0 41.0 30-31 37.08691314489659 39.5 36.0 41.0 30.5 41.0 32-33 36.90463610699712 39.0 36.0 41.0 30.0 41.0 34-35 36.75733062242282 39.0 35.5 41.0 30.0 41.0 36-37 36.65294075902801 39.0 35.0 41.0 30.0 41.0 38-39 36.5163315818959 39.0 35.0 41.0 30.0 41.0 40-41 36.351399371005684 39.0 35.0 40.5 29.0 41.0 42-43 36.14601178253713 39.0 35.0 40.0 28.0 41.0 44-45 35.93180979239495 38.5 35.0 40.0 27.5 41.0 46-47 35.70165014369018 38.0 34.5 40.0 27.0 41.0 48-49 35.498058437276256 38.0 34.0 40.0 26.0 41.0 50-51 34.82499424489238 37.5 33.5 39.5 25.5 40.5 52-53 34.929856748303614 38.0 33.5 40.0 26.0 40.5 54-55 35.40538331901528 38.0 34.0 40.0 26.0 41.0 56-57 35.36309599981075 38.0 34.0 40.0 26.0 41.0 58-59 35.14998295103456 38.0 34.0 40.0 26.0 41.0 60-61 34.92228781538422 37.0 34.0 40.0 26.0 41.0 62-63 34.62363003918089 37.0 33.5 40.0 26.0 41.0 64-65 34.371535497330726 36.0 33.0 40.0 26.0 41.0 66-67 34.1977587101174 36.0 33.0 39.0 26.0 41.0 68-69 33.905498825034925 35.5 33.0 39.0 25.0 41.0 70-71 33.60311179678787 35.0 33.0 39.0 25.0 40.5 72-73 33.272216186651264 35.0 32.5 37.5 24.0 40.0 74-75 32.94691471420914 35.0 32.0 37.0 24.0 39.5 76-77 32.61979566627414 35.0 32.0 36.5 23.5 39.0 78-79 32.28997411211238 35.0 31.5 36.0 23.0 39.0 80-81 32.00076630916727 35.0 31.0 36.0 22.5 38.0 82-83 31.715022705991025 35.0 31.0 35.0 21.5 37.0 84-85 31.47168475510935 35.0 31.0 35.0 20.5 37.0 86-87 31.248922915021147 34.0 31.0 35.0 20.0 36.0 88-89 31.035269895796493 34.0 31.0 35.0 20.0 36.0 90-91 30.81698025031689 34.0 30.0 35.0 19.5 36.0 92-93 30.62343231733808 34.0 30.0 35.0 19.0 35.5 94-95 30.453052053289703 34.0 30.0 35.0 18.0 35.0 96-97 30.2750490265935 34.0 29.0 35.0 18.0 35.0 98-99 30.043625735110425 34.0 29.0 35.0 17.5 35.0 100 28.910239475941918 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3952188887737691 1101 2 -0.33604537728083983 1101 3 -0.38504169953277767 1101 4 -0.24736567338017323 1101 5 -0.20775511334294094 1101 6 -0.2647959214538318 1101 7 -0.2571659592164579 1101 8 -0.2624642720873638 1101 9 -0.4400015633847758 1101 10-11 -0.39000551613185763 1101 12-13 -0.45519176738954314 1101 14-15 -0.5745910282110813 1101 16-17 -0.5956164103051123 1101 18-19 -0.500895385164263 1101 20-21 -0.5697249967544025 1101 22-23 -0.6031879259780695 1101 24-25 -0.7155968416148184 1101 26-27 -0.7032445733892843 1101 28-29 -0.6682887122076693 1101 30-31 -0.7430771651825765 1101 32-33 -0.7048915100389408 1101 34-35 -0.7588162983166242 1101 36-37 -0.7966645910062979 1101 38-39 -0.8160692674223142 1101 40-41 -0.8738455815451118 1101 42-43 -0.918491917824575 1101 44-45 -0.9356023326398528 1101 46-47 -0.9707984887891428 1101 48-49 -0.9476414079578817 1101 50-51 -1.0365572179340177 1101 52-53 -0.8839193087828789 1101 54-55 -0.763245589550472 1101 56-57 -0.6090431027363294 1101 58-59 -0.6319777395067376 1101 60-61 -0.6903892057194057 1101 62-63 -0.7527407139296898 1101 64-65 -0.7052003554001658 1101 66-67 -0.7510214185290138 1101 68-69 -0.7055100242524688 1101 70-71 -0.731774709175653 1101 72-73 -0.7838897223496133 1101 74-75 -0.7199693218296517 1101 76-77 -0.7594654629717397 1101 78-79 -0.7099230849959213 1101 80-81 -0.8574324593920117 1101 82-83 -0.9225708484387418 1101 84-85 -1.0481461415350175 1101 86-87 -1.1130387127108499 1101 88-89 -1.0687597590829512 1101 90-91 -1.0728201481934896 1101 92-93 -1.221954669878155 1101 94-95 -1.251711373244067 1101 96-97 -1.2182813289593781 1101 98-99 -1.3079612994095946 1101 100 -1.4320429633916198 1102 1 -0.4814782752873761 1102 2 -0.42367416637770106 1102 3 -0.46605091399945664 1102 4 -0.3250729776262631 1102 5 -0.31423950398060185 1102 6 -0.33976568832613907 1102 7 -0.3240840677114747 1102 8 -0.3663522605119738 1102 9 -0.4955660287058734 1102 10-11 -0.50575424617643 1102 12-13 -0.5830077121501702 1102 14-15 -0.700594113272814 1102 16-17 -0.7585495423676463 1102 18-19 -0.7512710060471122 1102 20-21 -0.7596394986774229 1102 22-23 -0.7380112035026656 1102 24-25 -0.7633112276019176 1102 26-27 -0.8570929917656542 1102 28-29 -0.8905298032044584 1102 30-31 -0.888485719591614 1102 32-33 -0.8560167189637227 1102 34-35 -0.9388282390596956 1102 36-37 -0.8830192390923983 1102 38-39 -0.9523809336062214 1102 40-41 -0.9706344340972421 1102 42-43 -0.9279843760239643 1102 44-45 -1.0536942419446476 1102 46-47 -1.0830424617770973 1102 48-49 -1.0573580920803138 1102 50-51 -1.1322688661600182 1102 52-53 -1.0447011830309663 1102 54-55 -0.7819013366189651 1102 56-57 -0.8996371080008032 1102 58-59 -0.8954727218814398 1102 60-61 -0.8541853970015794 1102 62-63 -0.9244715258122156 1102 64-65 -0.9374048336616951 1102 66-67 -0.8673857085516588 1102 68-69 -0.9100006560845699 1102 70-71 -0.9087276059030458 1102 72-73 -0.9743347209520579 1102 74-75 -0.9620892629265114 1102 76-77 -1.0422252363112818 1102 78-79 -1.018732465797008 1102 80-81 -1.0175802694814386 1102 82-83 -1.1003175325641585 1102 84-85 -0.9775799501514157 1102 86-87 -1.1797468243917528 1102 88-89 -1.2098213412196657 1102 90-91 -1.2091296796381776 1102 92-93 -1.1778637007535302 1102 94-95 -1.2260533645825085 1102 96-97 -1.2939110378771517 1102 98-99 -1.3781215733930274 1102 100 -1.4470865918680929 1103 1 -0.4605762568902385 1103 2 -0.3807846663159573 1103 3 -0.4986540858232651 1103 4 -0.37134805856526043 1103 5 -0.3291442876119106 1103 6 -0.4436352228433833 1103 7 -0.4068280818374177 1103 8 -0.3857887026457192 1103 9 -0.5678833152428169 1103 10-11 -0.5910413374738113 1103 12-13 -0.590422982155026 1103 14-15 -0.7718733789338117 1103 16-17 -0.7336241204441976 1103 18-19 -0.6447362958807545 1103 20-21 -0.7309114852355236 1103 22-23 -0.7192279169398219 1103 24-25 -0.7408657130052134 1103 26-27 -0.8066718708254115 1103 28-29 -0.9062474110262002 1103 30-31 -0.8028587843930595 1103 32-33 -0.8041014361724592 1103 34-35 -0.9087183975942139 1103 36-37 -0.9092351819381506 1103 38-39 -0.9337429690693497 1103 40-41 -0.9151475758100531 1103 42-43 -0.9969833851406165 1103 44-45 -1.0434321146166852 1103 46-47 -1.0691986715046369 1103 48-49 -1.0621125083473686 1103 50-51 -1.1091144953429648 1103 52-53 -1.0579621250074993 1103 54-55 -0.8971088764138173 1103 56-57 -0.8702577950785368 1103 58-59 -0.8035336172889203 1103 60-61 -0.8410689431552711 1103 62-63 -0.8439255443163773 1103 64-65 -0.7440883592495524 1103 66-67 -0.8714456062253007 1103 68-69 -0.8859454447257136 1103 70-71 -0.8467420187061165 1103 72-73 -0.847135661643847 1103 74-75 -0.8651488999584132 1103 76-77 -0.8751835885447576 1103 78-79 -0.9133448554506316 1103 80-81 -0.918352189740343 1103 82-83 -0.8973917904964708 1103 84-85 -1.0031680950303432 1103 86-87 -1.0323957865968403 1103 88-89 -1.0817772227186637 1103 90-91 -1.1850316885306995 1103 92-93 -1.2439160899222408 1103 94-95 -1.205185365545308 1103 96-97 -1.2593496457009294 1103 98-99 -1.2773660028707887 1103 100 -1.2379093664971315 1104 1 -0.4364132476616085 1104 2 -0.4474040797981189 1104 3 -0.4927530973050587 1104 4 -0.34469359814853817 1104 5 -0.28810392911955773 1104 6 -0.33246147598809017 1104 7 -0.37323070259486 1104 8 -0.3718231377294927 1104 9 -0.49072352689236 1104 10-11 -0.48176045019305036 1104 12-13 -0.4485417713709907 1104 14-15 -0.6674504564119488 1104 16-17 -0.7569823578427872 1104 18-19 -0.7385497236120884 1104 20-21 -0.7060355799479296 1104 22-23 -0.6226178014131207 1104 24-25 -0.7311636281355192 1104 26-27 -0.7890909758061042 1104 28-29 -0.7814996927154709 1104 30-31 -0.7411983292781557 1104 32-33 -0.8158133541173598 1104 34-35 -0.9121188084771958 1104 36-37 -0.8276477408110523 1104 38-39 -0.8406257601063274 1104 40-41 -0.8706008793790048 1104 42-43 -0.89879707192528 1104 44-45 -0.956731082112448 1104 46-47 -0.9996090902429131 1104 48-49 -0.9956026552800665 1104 50-51 -0.9977435741803475 1104 52-53 -0.8453391276212088 1104 54-55 -0.7118815188794585 1104 56-57 -0.6893961165788838 1104 58-59 -0.7627492298090459 1104 60-61 -0.7573359690159407 1104 62-63 -0.7741813644841855 1104 64-65 -0.7600036087884803 1104 66-67 -0.8873740718928573 1104 68-69 -0.7716364102766136 1104 70-71 -0.798592433413603 1104 72-73 -0.8442983783784541 1104 74-75 -0.8082229614901593 1104 76-77 -0.8135574350952908 1104 78-79 -0.839432523895141 1104 80-81 -0.7892113011253947 1104 82-83 -0.8667092348805063 1104 84-85 -0.8386254596960718 1104 86-87 -0.9309629808903885 1104 88-89 -1.0227742254212302 1104 90-91 -1.0084109322554333 1104 92-93 -1.044424414085789 1104 94-95 -0.9757001119724862 1104 96-97 -1.0910104169697448 1104 98-99 -1.1413624134563491 1104 100 -1.034680143654743 1105 1 -0.5035974134192003 1105 2 -0.45186119099285094 1105 3 -0.5042668668665478 1105 4 -0.33427730706385717 1105 5 -0.29324769036106346 1105 6 -0.32121811718073445 1105 7 -0.30319209997875163 1105 8 -0.33432926859257606 1105 9 -0.5349696892380607 1105 10-11 -0.5414089555944841 1105 12-13 -0.5294722914771697 1105 14-15 -0.6908354185856282 1105 16-17 -0.6867894298137287 1105 18-19 -0.7446919048320737 1105 20-21 -0.7171125092261619 1105 22-23 -0.6687837196074682 1105 24-25 -0.7519521208755435 1105 26-27 -0.7203122520062024 1105 28-29 -0.7191036522225573 1105 30-31 -0.7218030840165781 1105 32-33 -0.7688500534832414 1105 34-35 -0.7277641671062725 1105 36-37 -0.796437388927437 1105 38-39 -0.8010685073306689 1105 40-41 -0.8883445870711668 1105 42-43 -0.8761539522515847 1105 44-45 -0.9029498463981795 1105 46-47 -1.0068646598613782 1105 48-49 -1.0394453195080615 1105 50-51 -0.993253286289864 1105 52-53 -0.8008682821017814 1105 54-55 -0.6190584853768044 1105 56-57 -0.642243708778949 1105 58-59 -0.6322335648405897 1105 60-61 -0.6405301226632858 1105 62-63 -0.7308702306349417 1105 64-65 -0.6678347684304882 1105 66-67 -0.6486068530429776 1105 68-69 -0.6922169852434763 1105 70-71 -0.698101992496639 1105 72-73 -0.6030709048377787 1105 74-75 -0.6932294148952636 1105 76-77 -0.6807913890622892 1105 78-79 -0.8484243681697876 1105 80-81 -0.893733802677378 1105 82-83 -0.8402610708697473 1105 84-85 -0.8402215897015246 1105 86-87 -0.8370213632373975 1105 88-89 -0.9432442831058268 1105 90-91 -1.0295428162067601 1105 92-93 -1.024359429791442 1105 94-95 -1.032914084964208 1105 96-97 -1.082433124195731 1105 98-99 -0.9504623740464062 1105 100 -1.0952644966281788 1106 1 -0.6072467413533822 1106 2 -0.5653759497829363 1106 3 -0.6709836762420167 1106 4 -0.48306152396638424 1106 5 -0.41849184721365873 1106 6 -0.4731089300468909 1106 7 -0.46886876641958253 1106 8 -0.45008212317248564 1106 9 -0.5812312967482569 1106 10-11 -0.5486455771116354 1106 12-13 -0.6894165454517065 1106 14-15 -0.8682887105240056 1106 16-17 -0.8899034348520303 1106 18-19 -0.9026802561683738 1106 20-21 -0.8179979088943057 1106 22-23 -0.8560788367079155 1106 24-25 -0.8443022408473482 1106 26-27 -0.8707163474640964 1106 28-29 -0.914906222864964 1106 30-31 -1.0581483952951203 1106 32-33 -0.9730205938533558 1106 34-35 -0.9585341119838446 1106 36-37 -0.9970120086438712 1106 38-39 -1.0735343558613195 1106 40-41 -1.068114518693605 1106 42-43 -1.036066657079921 1106 44-45 -1.0514172399062502 1106 46-47 -1.0129027508177302 1106 48-49 -1.0942382269647268 1106 50-51 -1.0825491247520773 1106 52-53 -0.9060195644301459 1106 54-55 -0.8719436836824528 1106 56-57 -0.8548087451935018 1106 58-59 -0.9529413155350639 1106 60-61 -0.9352311547055123 1106 62-63 -0.8732511744114433 1106 64-65 -1.0274709881597985 1106 66-67 -0.9343444934242413 1106 68-69 -0.8979241262980437 1106 70-71 -0.8878795828909603 1106 72-73 -0.9289104306672726 1106 74-75 -0.9815535557017654 1106 76-77 -0.9292057315416535 1106 78-79 -0.8983416567713327 1106 80-81 -0.8958778688526543 1106 82-83 -0.9440482538092532 1106 84-85 -0.9183874959738567 1106 86-87 -0.9300832887552879 1106 88-89 -1.0328175566718727 1106 90-91 -0.9989376570451718 1106 92-93 -1.0530479702974453 1106 94-95 -1.17422225346181 1106 96-97 -1.2529675006172276 1106 98-99 -1.2111758526921754 1106 100 -1.400485540120524 1107 1 -0.6007699286226575 1107 2 -0.579555650928139 1107 3 -0.5781597060299681 1107 4 -0.5075357157448863 1107 5 -0.4785595883705511 1107 6 -0.5014884203750398 1107 7 -0.4768850588915967 1107 8 -0.48386167673961467 1107 9 -0.6440030411290536 1107 10-11 -0.6550031957069677 1107 12-13 -0.6974230085861706 1107 14-15 -0.8636477144272163 1107 16-17 -0.8346169686997555 1107 18-19 -0.8917857159130165 1107 20-21 -0.865656671097021 1107 22-23 -0.9140452924450742 1107 24-25 -0.9459338188746571 1107 26-27 -0.9965009233473552 1107 28-29 -1.0179050448778924 1107 30-31 -1.0502899549303706 1107 32-33 -1.057499257137053 1107 34-35 -1.0167493527014102 1107 36-37 -1.1145161726947563 1107 38-39 -1.092265592330655 1107 40-41 -1.1010064263426145 1107 42-43 -1.1136028945199143 1107 44-45 -1.1314672269180335 1107 46-47 -1.0641363250132798 1107 48-49 -1.0477223514345013 1107 50-51 -1.0137947487033898 1107 52-53 -1.0004865827857117 1107 54-55 -0.9033317946923134 1107 56-57 -0.9692893222078212 1107 58-59 -0.9664722804018524 1107 60-61 -0.9253444052352364 1107 62-63 -0.801954296828562 1107 64-65 -0.8064046016420292 1107 66-67 -0.8479784899899556 1107 68-69 -0.7520001271653527 1107 70-71 -0.833728581571286 1107 72-73 -0.7810537322880862 1107 74-75 -0.7852541709477592 1107 76-77 -0.7878500435447613 1107 78-79 -0.8492606308277075 1107 80-81 -0.854486283072287 1107 82-83 -0.8105127468991178 1107 84-85 -0.8489371384221194 1107 86-87 -0.8609061438512207 1107 88-89 -0.8561234680446788 1107 90-91 -0.8835453284674379 1107 92-93 -0.9795887151250824 1107 94-95 -0.9477443795919918 1107 96-97 -0.9657033165457811 1107 98-99 -1.0704238638997658 1107 100 -1.0838090645621428 1108 1 -0.5182492160279786 1108 2 -0.5326611360624085 1108 3 -0.4999836980804204 1108 4 -0.3029983641340124 1108 5 -0.24316841221372698 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58-59 1.1900787671897675 2311 60-61 1.1744967460355582 2311 62-63 1.2015696249800598 2311 64-65 1.1730580126570587 2311 66-67 1.2001582999157279 2311 68-69 1.1924970918569002 2311 70-71 1.1802523565812137 2311 72-73 1.1546699069227913 2311 74-75 1.1470843703439115 2311 76-77 1.1743221340778192 2311 78-79 1.1761290660547132 2311 80-81 1.1666063981862642 2311 82-83 1.1898402752766977 2311 84-85 1.1864226786993477 2311 86-87 1.1413280882749213 2311 88-89 1.2294271475503962 2311 90-91 1.1968215535975055 2311 92-93 1.290239701247586 2311 94-95 1.3345763868418778 2311 96-97 1.2948515740150945 2311 98-99 1.4275360774207009 2311 100 1.486519072812758 2312 1 0.6141650745008 2312 2 0.5808880325856123 2312 3 0.6319719267700776 2312 4 0.3671662683322836 2312 5 0.4366060366027469 2312 6 0.49329665447778126 2312 7 0.34428616892666497 2312 8 0.4897478861487059 2312 9 0.657719330068872 2312 10-11 0.6825996102644183 2312 12-13 0.7613021606646768 2312 14-15 0.9737228270371219 2312 16-17 1.0033474264347575 2312 18-19 0.9364660202793615 2312 20-21 0.9659871702562342 2312 22-23 1.024623321261764 2312 24-25 0.9718352372424874 2312 26-27 1.0186375586713297 2312 28-29 1.0373389366487658 2312 30-31 1.098082382385492 2312 32-33 1.0865624528606617 2312 34-35 1.0899812576558432 2312 36-37 1.1183751133638324 2312 38-39 1.144313651678793 2312 40-41 1.1253217883585833 2312 42-43 1.2036851037211775 2312 44-45 1.202298542440623 2312 46-47 1.2605118063829366 2312 48-49 1.2675718761477697 2312 50-51 1.197281044943722 2312 52-53 1.1145957660865236 2312 54-55 1.0435426919773079 2312 56-57 0.9989614536618987 2312 58-59 0.9399437881791428 2312 60-61 0.9222594332635268 2312 62-63 0.9531598185066699 2312 64-65 1.0054048640828128 2312 66-67 0.9434778995072932 2312 68-69 0.9245060781677594 2312 70-71 0.9346705268449327 2312 72-73 0.9028925548674067 2312 74-75 0.9597860873910733 2312 76-77 0.9797482303775311 2312 78-79 0.9102324957049106 2312 80-81 0.9106313558528889 2312 82-83 0.971241182571557 2312 84-85 0.9973592051192348 2312 86-87 1.0399292586485238 2312 88-89 0.9539515382100561 2312 90-91 1.0370344043679118 2312 92-93 1.1463110067303326 2312 94-95 1.1549106057161538 2312 96-97 1.1644081482748163 2312 98-99 0.8714675228843909 2312 100 1.0618266118822852 2313 1 0.5838865386747294 2313 2 0.5330295618298813 2313 3 0.6071767453680152 2313 4 0.4664914285501638 2313 5 0.32547753188007533 2313 6 0.45541634584424173 2313 7 0.51790887608513 2313 8 0.5263253059444892 2313 9 0.6421170840610699 2313 10-11 0.6908833287986056 2313 12-13 0.7696794011048311 2313 14-15 0.9676813516101319 2313 16-17 0.9233244002630414 2313 18-19 0.9193048332399201 2313 20-21 0.9309065310176337 2313 22-23 0.9476935643843163 2313 24-25 0.9102915045202167 2313 26-27 0.920195811604053 2313 28-29 1.0300363876260263 2313 30-31 0.9866222338328754 2313 32-33 0.9972575015787299 2313 34-35 1.0585366588155338 2313 36-37 1.0999917237012156 2313 38-39 1.1299521009526075 2313 40-41 1.1559508907348643 2313 42-43 1.2021025754525496 2313 44-45 1.2408064766843054 2313 46-47 1.2272640906397712 2313 48-49 1.2263517073862147 2313 50-51 1.2462278036868497 2313 52-53 1.1550349756567044 2313 54-55 0.9871919888659235 2313 56-57 0.9669873494707559 2313 58-59 0.9856035843986461 2313 60-61 0.9656332854781411 2313 62-63 0.9854794939145322 2313 64-65 1.0129426569234923 2313 66-67 0.9298043815367265 2313 68-69 0.9811814448748564 2313 70-71 1.0087396778751057 2313 72-73 0.9824271631333517 2313 74-75 0.9481038218306779 2313 76-77 0.8012020100453512 2313 78-79 0.9149556000684811 2313 80-81 1.018933725837588 2313 82-83 1.0275396166304382 2313 84-85 1.0435064929574942 2313 86-87 1.0552995359246005 2313 88-89 1.1368723474196756 2313 90-91 1.154375681450393 2313 92-93 1.1789206761345277 2313 94-95 1.2770732397482298 2313 96-97 1.2976871857514638 2313 98-99 1.3670966722366558 2313 100 1.3921736400161357 2314 1 0.5431884456362752 2314 2 0.4964735792218562 2314 3 0.5485997054839657 2314 4 0.4097805403378558 2314 5 0.40274065281001015 2314 6 0.3708548500370483 2314 7 0.46068269443843235 2314 8 0.4537259727275398 2314 9 0.6320200301476575 2314 10-11 0.6077931571410673 2314 12-13 0.6724757729643116 2314 14-15 0.8725554592470601 2314 16-17 0.8057171032404682 2314 18-19 0.7691920063662181 2314 20-21 0.7792460255736628 2314 22-23 0.8433748677222681 2314 24-25 0.8188818659668726 2314 26-27 0.8815310281546118 2314 28-29 0.8705199366111458 2314 30-31 0.934617806211584 2314 32-33 0.8699407671137962 2314 34-35 0.8838579030465965 2314 36-37 0.849100592445204 2314 38-39 0.860216227545429 2314 40-41 0.9696469316628438 2314 42-43 1.018900582152611 2314 44-45 1.0671145379631994 2314 46-47 1.0545561187131653 2314 48-49 0.9260857005253271 2314 50-51 0.9973480611440237 2314 52-53 0.9352716409128163 2314 54-55 0.87154033427678 2314 56-57 0.8721689158232451 2314 58-59 0.8371477977082264 2314 60-61 0.814570622919284 2314 62-63 0.8337089794813934 2314 64-65 0.8348866027084725 2314 66-67 0.8412566580817256 2314 68-69 0.8484951927778113 2314 70-71 0.9182217550145779 2314 72-73 0.9263327028965236 2314 74-75 0.9172024181537424 2314 76-77 0.8792252494825235 2314 78-79 0.8786004749029743 2314 80-81 0.8770193874412691 2314 82-83 0.8983386758921945 2314 84-85 1.0005365412093177 2314 86-87 1.0355220416256508 2314 88-89 1.066565660880002 2314 90-91 1.0866403100474535 2314 92-93 1.125971007400505 2314 94-95 1.1272781037298252 2314 96-97 1.211738722503764 2314 98-99 1.3781166777422307 2314 100 1.3712222999007437 2315 1 0.45385669106396165 2315 2 0.44439749792621797 2315 3 0.46949011192522505 2315 4 0.18724359484996 2315 5 0.2982884095393956 2315 6 0.32259737057756155 2315 7 0.36997316483217446 2315 8 0.3894000356152034 2315 9 0.526250429001152 2315 10-11 0.41906443967860696 2315 12-13 0.4788202737101628 2315 14-15 0.6537001744342419 2315 16-17 0.6614045739882641 2315 18-19 0.571005470897127 2315 20-21 0.6749233230218437 2315 22-23 0.47323674723936904 2315 24-25 0.621283355658754 2315 26-27 0.7424247562204158 2315 28-29 0.7107504821543316 2315 30-31 0.7506477182452542 2315 32-33 0.8329028066704112 2315 34-35 0.8458200254459598 2315 36-37 0.8999195747247697 2315 38-39 0.9101914802553708 2315 40-41 0.9101805391687137 2315 42-43 1.0142720869936355 2315 44-45 1.0291048374476262 2315 46-47 0.9828553176713513 2315 48-49 1.0121570392059738 2315 50-51 0.8882049532115417 2315 52-53 0.9187733376060123 2315 54-55 0.8185758173528441 2315 56-57 0.7911369221678441 2315 58-59 0.7736460271425187 2315 60-61 0.7693554639641178 2315 62-63 0.7772715049080361 2315 64-65 0.7699330263029296 2315 66-67 0.8347286633034585 2315 68-69 0.8711075937556743 2315 70-71 0.7712064813459563 2315 72-73 0.5463796172154929 2315 74-75 0.7003448965869197 2315 76-77 0.7158096986864635 2315 78-79 0.7872007254363069 2315 80-81 0.5621588903953842 2315 82-83 0.5497137862392485 2315 84-85 0.8015732890882568 2315 86-87 0.9305164009320315 2315 88-89 0.9292430010687021 2315 90-91 0.9860878887968703 2315 92-93 1.033627444307843 2315 94-95 1.0790768597167961 2315 96-97 0.8929087522163393 2315 98-99 0.31763521103524894 2315 100 0.8684897294593519 2316 1 0.21369890881813447 2316 2 0.2183631606149845 2316 3 0.2563369677838736 2316 4 0.1402793025860305 2316 5 0.061070205229398766 2316 6 0.0993828407399775 2316 7 0.07555322798508968 2316 8 0.13215749042619507 2316 9 0.20034886618334724 2316 10-11 0.13122694338809282 2316 12-13 -0.018030750107222104 2316 14-15 -0.02654376785453394 2316 16-17 0.1390763855831949 2316 18-19 0.15111143707996888 2316 20-21 0.20342715921105992 2316 22-23 0.20632453778777204 2316 24-25 0.24930891024733626 2316 26-27 0.264425576474693 2316 28-29 0.2797608086888914 2316 30-31 0.1942104226836321 2316 32-33 0.3892512080081616 2316 34-35 0.3908118395750009 2316 36-37 0.45955939640281684 2316 38-39 0.5641205982819528 2316 40-41 0.6000285253777733 2316 42-43 0.5266759321856469 2316 44-45 0.46505111412673017 2316 46-47 0.2960542760151057 2316 48-49 0.25432697855383424 2316 50-51 0.370316874573831 2316 52-53 0.4897251993498202 2316 54-55 0.1542337379749128 2316 56-57 0.018396439284813937 2316 58-59 -0.0019554093027807085 2316 60-61 0.13458721914155802 2316 62-63 0.29911254229629947 2316 64-65 0.23992345666967907 2316 66-67 0.18588732248780104 2316 68-69 0.17558158069162033 2316 70-71 -0.029792628378054076 2316 72-73 -0.16854220855049107 2316 74-75 0.0753340098787092 2316 76-77 0.32148205711758493 2316 78-79 0.28962987847374677 2316 80-81 0.4602588347114569 2316 82-83 0.4824327291115402 2316 84-85 0.5164853118152983 2316 86-87 0.5782710048592463 2316 88-89 0.5530549827830349 2316 90-91 0.4400800263595137 2316 92-93 0.4680271082036498 2316 94-95 0.5906938609871908 2316 96-97 0.5596421062227499 2316 98-99 0.2916809652190473 2316 100 0.20113458269718976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 35.0 1 525.0 2 204.0 3 182.0 4 172.0 5 129.0 6 134.0 7 705.0 8 2375.0 9 4214.0 10 6350.0 11 9623.0 12 14024.0 13 17735.0 14 21237.0 15 24745.0 16 29703.0 17 36223.0 18 43942.0 19 52849.0 20 64099.0 21 77369.0 22 86277.0 23 87976.0 24 87172.0 25 90916.0 26 99612.0 27 114178.0 28 133785.0 29 159432.0 30 193686.0 31 242113.0 32 306891.0 33 403689.0 34 573022.0 35 798265.0 36 1209415.0 37 1732092.0 38 1532148.0 39 408578.0 40 394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94334536008082 19.540597488815127 12.956224563429068 23.55983258767499 2 15.67479973517555 19.44756602990945 41.428457536231384 23.44917669868362 3 19.50156329146283 25.483237736982257 28.693181588672516 26.3220173828824 4 12.736844850811519 16.029348356973962 38.24328797250098 32.99051881971354 5 13.461402850916146 37.50653960586129 34.42024958586824 14.611807957354324 6 30.966534008328022 36.82713780677217 17.92010920847294 14.28621897642687 7 27.877727059391315 31.773068479058413 22.26074818311499 18.088456278435284 8 26.09271535411677 35.64335712210129 19.742861031795336 18.521066491986602 9 26.332873772440824 15.979432081552261 21.003756716936696 36.68393742907023 10-11 25.818516081729157 26.05019234977131 27.300421131456815 20.830870437042716 12-13 25.85518814961789 23.59424935156896 27.86340954585383 22.68715295295932 14-15 23.116844641114724 25.003397450068736 25.699853074350404 26.179904834466132 16-17 22.210513760270366 27.781087192725728 26.70287576329333 23.305523283710574 18-19 21.803116280701627 27.360258095848344 28.870629848414627 21.965995775035406 20-21 22.95943502500784 26.594540742569855 28.58605000575055 21.859974226671753 22-23 23.050990246828494 26.410312108182517 28.293604153578432 22.245093491410557 24-25 22.56866053447366 26.313095949916764 28.890918474504407 22.227325041105168 26-27 22.521429943198186 26.580868028112835 28.246305158328592 22.651396870360387 28-29 22.050961029485297 26.94715377977886 28.567671099568415 22.43421409116743 30-31 22.796247381784898 26.618296120027097 28.50295512510016 22.082501373087847 32-33 22.82837868306487 26.779972370214654 27.993125274513208 22.39852367220726 34-35 22.86634988383219 26.74666068034057 28.410647735102764 21.976341700724475 36-37 22.647187768905297 26.53675977050106 28.186569772706115 22.629482687887524 38-39 22.67570431274681 26.747123168666825 28.409827141688233 22.16734537689813 40-41 22.671297610530612 26.11651624505506 28.699827153313585 22.512358991100744 42-43 22.499561517190344 26.293547374121385 28.28879277184102 22.918098336847255 44-45 22.512863838091253 26.88161932759242 28.228357707483305 22.377159126833025 46-47 22.53728719030185 26.671835205240164 27.773240731408816 23.01763687304917 48-49 22.940937685475014 26.64485943710428 27.772362814073425 22.64184006334728 50-51 22.752782029677945 26.661079032133056 27.736459176824045 22.849679761364953 52-53 22.50111497265568 27.015041438542426 27.51185720823545 22.971986380566445 54-55 22.403267248592847 26.93027724932123 27.59255409370513 23.073901408380795 56-57 22.94452421632163 26.704588545840956 27.273930418427 23.076956819410412 58-59 22.417855821885073 26.94403386015876 27.732274880137865 22.905835437818297 60-61 22.563517073741906 27.26524719376244 27.39277734634215 22.7784583861535 62-63 22.7037408626696 26.893283136988757 27.24930322129761 23.153672779044037 64-65 22.284185336250452 26.845206076551833 27.493842209951946 23.376766377245772 66-67 22.556050291888024 26.65303849228221 27.343412103180903 23.447499112648863 68-69 22.29066594773339 26.766060231209543 27.530215427756353 23.413058393300716 70-71 22.365438213806485 26.486024238136974 27.570742280246645 23.577795267809893 72-73 22.716675176646046 26.705440849447942 27.535039739083224 23.04284423482279 74-75 22.86893939861745 26.885735722729187 27.228028838448925 23.017296040204435 76-77 22.7059478403622 26.77701349158324 27.081510989920986 23.435527678133575 78-79 22.54804238508338 26.799823256028137 27.15323041041961 23.49890394846887 80-81 22.38165398671778 27.092641697729093 27.364018938524588 23.16168537702854 82-83 22.951910141718702 26.560432653377784 27.949279865123017 22.538377339780492 84-85 23.058946537505452 26.834217335207512 27.157426192767804 22.949409934519235 86-87 22.340838419714878 27.261243203667917 27.435306166162416 22.96261221045479 88-89 22.87023385316733 26.513761414948718 27.397735043351556 23.218269688532395 90-91 22.88387588664515 26.535708984878585 27.40317219815055 23.17724293032572 92-93 23.007256747311555 26.719922746733566 27.31208699233007 22.96073351362481 94-95 22.412528480823827 26.7075769465556 27.6326461078814 23.24724846473918 96-97 22.618890085466248 26.644587727225222 27.359067716897506 23.377454470411028 98-99 22.331199206076498 26.90476022838459 27.20775635930025 23.55628420623867 100 22.897564701837076 26.5735217377604 26.703766276383206 23.825147284019316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1605.0 1 1149.0 2 1503.0 3 2132.5 4 1516.0 5 1212.5 6 1731.5 7 3080.0 8 5042.0 9 5971.0 10 5368.0 11 4762.5 12 4969.5 13 5725.0 14 6681.5 15 7523.0 16 8423.5 17 9343.5 18 10259.5 19 11044.5 20 11907.5 21 13052.5 22 14651.0 23 17265.5 24 20647.0 25 24490.5 26 29845.0 27 36731.0 28 45869.5 29 60512.0 30 79410.0 31 97705.0 32 120845.0 33 145316.0 34 165976.5 35 187064.0 36 207590.5 37 235944.0 38 251345.0 39 249813.0 40 246970.0 41 242111.0 42 245637.5 43 259125.0 44 277353.5 45 307625.5 46 336920.0 47 357525.0 48 375085.0 49 379363.0 50 372535.0 51 374021.0 52 370228.0 53 353498.0 54 333793.0 55 298524.0 56 265323.0 57 227611.5 58 189918.5 59 169933.5 60 141254.0 61 107702.0 62 83834.0 63 66967.5 64 49610.5 65 31695.5 66 21537.0 67 15651.0 68 11997.0 69 9368.0 70 7411.5 71 5649.0 72 4286.5 73 3288.5 74 2456.5 75 1807.0 76 1297.0 77 843.5 78 545.5 79 405.0 80 300.5 81 209.0 82 156.5 83 118.5 84 83.0 85 71.0 86 66.0 87 63.5 88 51.5 89 36.0 90 29.0 91 26.0 92 19.5 93 18.5 94 16.0 95 7.5 96 4.5 97 4.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05729144730427298 2 0.13183379364578424 3 0.017123969345325497 4 0.01697396152761038 5 0.01963948505777897 6 0.05326431435176718 7 0.05884922079592994 8 0.04943334546858115 9 0.041517548318383515 10-11 0.040161708427496896 12-13 0.04124638034020619 14-15 0.018791363934543514 16-17 0.017787465462142354 18-19 0.017620149050075494 20-21 0.021133793703479547 22-23 0.02612443840823243 24-25 0.02490706727215976 26-27 0.019651024120680136 28-29 0.018168254537880724 30-31 0.016593172451872012 32-33 0.02324544221439233 34-35 0.023366602374854537 36-37 0.023487762535316745 38-39 0.021514582779217917 40-41 0.025137848530183016 42-43 0.032534387849828324 44-45 0.04219258349810153 46-47 0.04837175168167418 48-49 0.040288638119409685 50-51 0.028172622073188817 52-53 0.03976938028885736 54-55 0.036301891887057965 56-57 0.038286610706057954 58-59 0.04342149369707537 60-61 0.02295696564186326 62-63 0.025818653241351616 64-65 0.0252647782220958 66-67 0.03219398549424403 68-69 0.03900780213738062 70-71 0.040421337342773055 72-73 0.044263845288860244 74-75 0.026487918889619055 76-77 0.020493375712465015 78-79 0.019668332715031878 80-81 0.023960864114264416 82-83 0.019927961630308043 84-85 0.012404492618749938 86-87 0.014389211437749929 88-89 0.018347110012848746 90-91 0.020920321039808037 92-93 0.019927961630308043 94-95 0.02367815707318593 96-97 0.02482629383185162 98-99 0.027786063465999867 100 0.028986126007720787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8666215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.58688660118644 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76117681221643 48.810042453320285 2 13.881952142804618 17.654202334152284 3 4.4871520145156305 8.559720789278721 4 1.9280973401015973 4.904068276843578 5 0.9708556950108218 3.0866845492384587 6 0.5345465040655625 2.0394088762246536 7 0.3419222778063581 1.521924117370458 8 0.2166231152383659 1.1019511571086171 9 0.16462097193279102 0.9420961567020729 >10 0.6712957570532988 7.165474234268995 >50 0.028461748014718832 1.247788082840566 >100 0.012245428614008935 1.3828010248106222 >500 6.534771734808499E-4 0.27170532021891214 >1k 3.03370640473866E-4 0.33272962708152154 >5k 2.333620295938859E-5 0.07751770790451493 >10k+ 7.000860887816577E-5 0.9018852926356266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26494 0.30571593250340545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21164 0.2442127272402081 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13121 0.15140404432615623 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.3078125802325468E-5 5 0.0 0.0 0.0 0.0 2.3078125802325468E-5 6 0.0 0.0 0.0 0.0 4.6156251604650935E-5 7 0.0 0.0 0.0 0.0 4.6156251604650935E-5 8 0.0 0.0 0.0 0.0 6.92343774069764E-5 9 0.0 0.0 0.0 0.0 1.0385156611046461E-4 10-11 0.0 0.0 0.0 2.3078125802325468E-5 1.0385156611046461E-4 12-13 0.0 5.769531450581367E-6 0.0 3.46171887034882E-5 1.0962109756104598E-4 14-15 0.0 1.1539062901162734E-5 0.0 4.038672015406957E-5 2.077031322209292E-4 16-17 0.0 1.1539062901162734E-5 0.0 6.346484595639504E-5 2.423203209244174E-4 18-19 0.0 1.1539062901162734E-5 0.0 9.231250320930187E-5 2.5385938382558015E-4 20-21 0.0 1.1539062901162734E-5 0.0 1.73085943517441E-4 2.6539844672674286E-4 22-23 0.0 1.1539062901162734E-5 0.0 3.1732422978197514E-4 2.942461039796497E-4 24-25 0.0 1.1539062901162734E-5 0.0 6.808047111686014E-4 3.115546983313938E-4 26-27 0.0 1.1539062901162734E-5 0.0 0.0016443164634156896 3.2309376123255653E-4 28-29 0.0 1.1539062901162734E-5 0.0 0.004055980609758701 3.2309376123255653E-4 30-31 0.0 1.1539062901162734E-5 0.0 0.01464884035302609 3.404023555843006E-4 32-33 0.0 1.1539062901162734E-5 0.0 0.040300177182310847 3.577109499360447E-4 34-35 0.0 1.1539062901162734E-5 0.0 0.07814830349812461 3.577109499360447E-4 36-37 0.0 1.1539062901162734E-5 0.0 0.14016499706042373 3.577109499360447E-4 38-39 0.0 1.1539062901162734E-5 0.0 0.2313293635110599 3.634804813866261E-4 40-41 0.0 1.1539062901162734E-5 0.0 0.32888637080894023 3.692500128372075E-4 42-43 0.0 1.1539062901162734E-5 0.0 0.4242567256870502 3.7501954428778887E-4 44-45 0.0 1.1539062901162734E-5 0.0 0.5389492413931571 3.807890757383702E-4 46-47 0.0 1.1539062901162734E-5 0.0 0.6760563867847729 3.9232813863953296E-4 48-49 0.0 1.1539062901162734E-5 0.0 0.8233929114382692 4.2694532734302115E-4 50-51 0.0 1.1539062901162734E-5 0.0 0.9743700104370823 4.2694532734302115E-4 52-53 0.0 1.1539062901162734E-5 0.0 1.1197795115860845 4.6156251604650934E-4 54-55 0.0 1.1539062901162734E-5 0.0 1.2831784117979994 4.961797047499975E-4 56-57 0.0 1.1539062901162734E-5 0.0 1.4723440394682106 4.961797047499975E-4 58-59 0.0 1.1539062901162734E-5 0.0 1.6877956524272708 4.961797047499975E-4 60-61 0.0 1.1539062901162734E-5 0.0 1.9059012498535983 5.077187676511603E-4 62-63 0.0 1.73085943517441E-5 0.0 2.1225471558229287 5.192578305523231E-4 64-65 0.0 2.3078125802325468E-5 0.0 2.3423547650271774 5.192578305523231E-4 66-67 0.0 2.3078125802325468E-5 0.0 2.590311918178813 5.192578305523231E-4 68-69 0.0 2.3078125802325468E-5 0.0 2.8666436270044073 5.192578305523231E-4 70-71 0.0 2.3078125802325468E-5 0.0 3.1620263286798216 5.769531450581367E-4 72-73 0.0 2.3078125802325468E-5 0.0 3.4552166084040152 6.346484595639504E-4 74-75 0.0 2.3078125802325468E-5 0.0 3.7425046574542638 6.346484595639504E-4 76-77 0.0 2.3078125802325468E-5 0.0 4.065436871806204 6.519570539156945E-4 78-79 0.0 2.3078125802325468E-5 0.0 4.416957114495775 6.692656482674386E-4 80-81 0.0 2.3078125802325468E-5 0.0 4.7866571507861275 6.808047111686014E-4 82-83 0.0 2.3078125802325468E-5 0.0 5.161070894271605 7.558086200261591E-4 84-85 0.0 2.3078125802325468E-5 0.0 5.531128641511894 7.615781514767404E-4 86-87 0.0 2.3078125802325468E-5 0.0 5.921275897263108 7.673476829273217E-4 88 0.0 2.3078125802325468E-5 0.0 6.233840263598353 7.846562772790659E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14390 0.0 45.851433 1 GTATCAA 24450 0.0 39.054302 1 TACCTGG 5645 0.0 33.096893 2 CAACGCA 28215 0.0 32.27276 5 ATCAACG 28810 0.0 31.638878 3 GTACTAG 5720 0.0 31.346905 1 TATCAAC 30935 0.0 30.593039 2 AACGCAG 31490 0.0 29.603231 6 TCAACGC 32000 0.0 28.719759 4 GTACATG 31920 0.0 27.894844 1 GCATCGT 5690 0.0 27.840954 8 ACGCAGA 34090 0.0 26.766409 7 TACATGG 32430 0.0 26.643202 2 GTGGTAT 5690 0.0 26.218794 1 CGCAGAG 34890 0.0 26.083801 8 ACATGGG 32340 0.0 25.888712 3 TAGGCAT 7340 0.0 25.874493 5 CTAACGC 2620 0.0 25.119633 3 TCTAACG 2665 0.0 25.075628 2 ACCTGGG 7330 0.0 25.011929 3 >>END_MODULE