FastQCFastQC Report
Mon 6 Jun 2016
ERR1211211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8666215
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245010.2827185801413881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203210.23448529721452788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128660.14846158328635972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146150.046.4263731
GTATCAA245200.038.4878961
GTACTAG57350.031.9763161
CAACGCA290500.031.2490865
ATCAACG291900.031.0475433
TACCTGG64150.030.9191462
TATCAAC313150.029.8083922
AACGCAG328600.028.1406356
TCAACGC327150.028.0614194
GTGGTAT55350.026.9301571
TAGTACT82900.026.763434
CTGTGCG80650.026.3333079
TAGGCAT76650.025.9914235
GGGCACC72250.025.89701394
GTACCTG78200.025.6153371
ACGCAGA356850.025.5047047
GTACATG315000.025.1080321
CGCAGAG363000.025.007598
ACCTGGG78300.024.6737043
CTAGGCA83250.024.6182144
Produced by FastQC (version 0.11.5)