##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8666215 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14663575736351 33.0 31.0 34.0 30.0 34.0 2 32.288682198629964 34.0 31.0 34.0 30.0 34.0 3 32.366021959990604 34.0 31.0 34.0 30.0 34.0 4 35.98353144942746 37.0 35.0 37.0 35.0 37.0 5 35.866689437084126 37.0 35.0 37.0 35.0 37.0 6 35.894066094598394 37.0 35.0 37.0 35.0 37.0 7 35.83540449896523 37.0 35.0 37.0 35.0 37.0 8 35.8232594044805 37.0 35.0 37.0 35.0 37.0 9 37.43308560888462 39.0 37.0 39.0 34.0 39.0 10-11 37.40796760754263 39.0 37.0 39.0 34.0 39.0 12-13 37.30793916375257 39.0 37.0 39.0 34.0 39.0 14-15 38.53249480886408 40.0 38.0 41.0 34.0 41.0 16-17 38.483127062968094 40.0 38.0 41.0 34.0 41.0 18-19 38.504203045966435 40.0 38.0 41.0 34.0 41.0 20-21 38.46408587832174 40.0 38.0 41.0 34.0 41.0 22-23 38.336225560985966 40.0 38.0 41.0 34.0 41.0 24-25 38.27197582797103 40.0 38.0 41.0 34.0 41.0 26-27 38.12952921200316 40.0 38.0 41.0 33.0 41.0 28-29 38.11137382352042 40.0 37.5 41.0 33.5 41.0 30-31 38.125183485523955 40.0 37.5 41.0 33.5 41.0 32-33 37.99877293605109 40.0 37.0 41.0 33.0 41.0 34-35 37.85936640159516 40.0 37.0 41.0 33.0 41.0 36-37 37.71738694458884 40.0 37.0 41.0 33.0 41.0 38-39 37.62818918062845 40.0 37.0 41.0 33.0 41.0 40-41 37.53073867888115 40.0 36.5 41.0 32.5 41.0 42-43 37.38883105254139 40.0 36.0 41.0 32.0 41.0 44-45 37.248305748241876 40.0 36.0 41.0 31.5 41.0 46-47 37.01553671354796 39.0 36.0 41.0 31.0 41.0 48-49 36.78089154261693 39.0 35.0 41.0 30.5 41.0 50-51 36.579411484713916 39.0 35.0 41.0 30.0 41.0 52-53 36.37631001538734 39.0 35.0 40.0 30.0 41.0 54-55 36.1329721798963 38.0 35.0 40.0 29.5 41.0 56-57 35.871799511089904 38.0 35.0 40.0 29.0 41.0 58-59 35.5920006600344 38.0 34.0 40.0 28.0 41.0 60-61 35.32614226626042 37.0 34.0 40.0 28.0 41.0 62-63 35.01920925109751 37.0 34.0 40.0 28.0 41.0 64-65 34.6409205748992 36.0 34.0 39.0 27.0 41.0 66-67 34.33408616102878 36.0 33.0 39.0 26.0 40.0 68-69 34.00612447302542 35.0 33.0 38.5 26.0 40.0 70-71 33.68418444499704 35.0 33.0 38.0 26.0 40.0 72-73 33.344875184841364 35.0 32.5 37.0 26.0 39.5 74-75 33.01087925928448 35.0 32.0 37.0 25.5 39.0 76-77 32.02852537122608 34.0 30.5 36.0 24.5 38.0 78-79 32.531718460712085 35.0 32.0 36.0 25.0 38.0 80-81 32.464407472004794 35.0 32.0 35.5 25.0 37.0 82-83 32.21962419579943 35.0 32.0 35.0 25.0 37.0 84-85 31.97234594341359 34.0 31.5 35.0 25.0 36.5 86-87 31.739275796873258 34.0 31.0 35.0 24.5 36.0 88-89 31.526957789530954 34.0 31.0 35.0 24.0 36.0 90-91 31.314141352366633 34.0 31.0 35.0 23.5 35.0 92-93 31.132642681955154 34.0 31.0 35.0 23.0 35.0 94-95 30.94599320464586 34.0 30.5 35.0 23.0 35.0 96-97 30.77767024012213 34.0 30.0 35.0 20.5 35.0 98-99 30.602902708968102 34.0 30.0 35.0 20.0 35.0 100 28.788309429202943 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3233655898577723 1101 2 -0.3302273517096097 1101 3 -0.3649817771692838 1101 4 -0.18169023755658031 1101 5 -0.25959496481255684 1101 6 -0.2770243467891689 1101 7 -0.2993926043532582 1101 8 -0.25862352092364915 1101 9 -0.41741336928333794 1101 10-11 -0.45772577266370007 1101 12-13 -0.4431034787734589 1101 14-15 -0.642546925913166 1101 16-17 -0.5809298345760894 1101 18-19 -0.6709149405704409 1101 20-21 -0.7148588363364894 1101 22-23 -0.7432901477269525 1101 24-25 -0.6600669245895219 1101 26-27 -0.7637633873426708 1101 28-29 -0.7800796866335844 1101 30-31 -0.6419438775345299 1101 32-33 -0.6210388426722986 1101 34-35 -0.5729304986050536 1101 36-37 -0.6073545308373411 1101 38-39 -0.7025882186832106 1101 40-41 -0.7635575491318889 1101 42-43 -0.7158040143176834 1101 44-45 -0.7687817210118126 1101 46-47 -0.6697320333510675 1101 48-49 -0.7425801060203341 1101 50-51 -0.8287369024112152 1101 52-53 -0.8038476795332343 1101 54-55 -0.7138731326241015 1101 56-57 -0.6716342259852794 1101 58-59 -0.6470528018556934 1101 60-61 -0.7853941870580954 1101 62-63 -0.8133009726316089 1101 64-65 -0.7678288922472589 1101 66-67 -0.8857906392564772 1101 68-69 -0.9719315122152565 1101 70-71 -0.9495243961378819 1101 72-73 -1.107188401607388 1101 74-75 -1.0725545870146753 1101 76-77 -0.9135465152614017 1101 78-79 -0.7990861418393749 1101 80-81 -0.7647742005790157 1101 82-83 -0.7475598255376319 1101 84-85 -0.6356035256469603 1101 86-87 -0.7983920007601988 1101 88-89 -0.816979259285997 1101 90-91 -0.8747323613668136 1101 92-93 -0.9094529997682486 1101 94-95 -0.887677032723623 1101 96-97 -1.0185995858996648 1101 98-99 -1.13653810612146 1101 100 -0.9520718205961956 1102 1 -0.40977021321445406 1102 2 -0.33905230771719985 1102 3 -0.390533487004042 1102 4 -0.2974417642027518 1102 5 -0.4086460741610196 1102 6 -0.4205938746303417 1102 7 -0.3903257646801137 1102 8 -0.40096319660138846 1102 9 -0.5024903153526239 1102 10-11 -0.5456726565484971 1102 12-13 -0.5083966219090428 1102 14-15 -0.7080332396432354 1102 16-17 -0.7897264311790622 1102 18-19 -0.7035632134042018 1102 20-21 -0.7323119794631339 1102 22-23 -0.800849923035301 1102 24-25 -0.8072250516032611 1102 26-27 -0.892569357035562 1102 28-29 -0.9392942521252152 1102 30-31 -0.8353511297712899 1102 32-33 -0.7777198362668116 1102 34-35 -0.7631773020521351 1102 36-37 -0.8956972438481472 1102 38-39 -0.9667065542324522 1102 40-41 -0.8952917471396376 1102 42-43 -0.8350757818713177 1102 44-45 -0.9074949222093309 1102 46-47 -0.8910613669723659 1102 48-49 -0.9450709066925853 1102 50-51 -0.9810449526265472 1102 52-53 -0.9975627537080527 1102 54-55 -0.9539891315567672 1102 56-57 -0.9868099025316823 1102 58-59 -1.0637785358554765 1102 60-61 -1.0308093172096946 1102 62-63 -1.072539844319948 1102 64-65 -1.0062380692916761 1102 66-67 -1.096737235203186 1102 68-69 -1.1024869892588072 1102 70-71 -1.0849250600287306 1102 72-73 -1.0833737728901838 1102 74-75 -1.2214430553361133 1102 76-77 -0.9667495674798445 1102 78-79 -0.8432451149928966 1102 80-81 -0.7983970331382011 1102 82-83 -0.7551907445262742 1102 84-85 -0.8515671502872451 1102 86-87 -0.9589581917709218 1102 88-89 -1.0668663977674449 1102 90-91 -0.9738256043149782 1102 92-93 -1.0591407461488522 1102 94-95 -1.1536471846320389 1102 96-97 -1.166521567352305 1102 98-99 -1.122501878795184 1102 100 -1.2528225515159903 1103 1 -0.38722335786760453 1103 2 -0.3791135023946275 1103 3 -0.407600087083388 1103 4 -0.24548704621248874 1103 5 -0.2920253411024589 1103 6 -0.39036282238266296 1103 7 -0.4456068659160266 1103 8 -0.39924537135703275 1103 9 -0.562883520332484 1103 10-11 -0.5788005998642447 1103 12-13 -0.6395061860190054 1103 14-15 -0.7653306470021732 1103 16-17 -0.7664212238641497 1103 18-19 -0.7134418114536558 1103 20-21 -0.7571231756021177 1103 22-23 -0.7825124120337108 1103 24-25 -0.8833403118488548 1103 26-27 -0.8164326755324893 1103 28-29 -0.9374745033003578 1103 30-31 -0.8583006132992992 1103 32-33 -0.761288637848871 1103 34-35 -0.8977064932991539 1103 36-37 -0.8314630612454934 1103 38-39 -0.796512968831145 1103 40-41 -0.8575540281196083 1103 42-43 -0.7845358890697796 1103 44-45 -0.830248459962732 1103 46-47 -0.8378225750602724 1103 48-49 -0.8712147058993835 1103 50-51 -0.9066701852431152 1103 52-53 -0.909656862125189 1103 54-55 -0.9502062486712006 1103 56-57 -0.9604828094524507 1103 58-59 -0.9560449974328122 1103 60-61 -0.9771582938653864 1103 62-63 -0.8966645147114463 1103 64-65 -0.8038495712856886 1103 66-67 -1.005935429913933 1103 68-69 -0.999673910990218 1103 70-71 -1.0876840982660951 1103 72-73 -0.9459948012016355 1103 74-75 -0.8944876406393973 1103 76-77 -0.8364414668515465 1103 78-79 -0.7722940993921554 1103 80-81 -0.6927511303083236 1103 82-83 -0.8553766028523029 1103 84-85 -0.8868954575545516 1103 86-87 -0.8662637390283656 1103 88-89 -0.8752110885217768 1103 90-91 -0.958148938084566 1103 92-93 -1.0052221767682035 1103 94-95 -0.9502105603682836 1103 96-97 -0.9622144186922235 1103 98-99 -1.0311058555346229 1103 100 -1.0630556395452864 1104 1 -0.36851028403233954 1104 2 -0.3344224100722144 1104 3 -0.32411200307213406 1104 4 -0.1859473449448643 1104 5 -0.20504782029940571 1104 6 -0.31955207944544384 1104 7 -0.3413308533634094 1104 8 -0.3236555902088867 1104 9 -0.5129718763945164 1104 10-11 -0.5444266202397827 1104 12-13 -0.5572309982216908 1104 14-15 -0.6811536023310367 1104 16-17 -0.646003875569555 1104 18-19 -0.7146956303121357 1104 20-21 -0.7540747872827467 1104 22-23 -0.689857173492662 1104 24-25 -0.7798208817634489 1104 26-27 -0.7739900728777798 1104 28-29 -0.8712933745269069 1104 30-31 -0.742077676788135 1104 32-33 -0.8238310004917935 1104 34-35 -0.8473775640907277 1104 36-37 -0.7718675508926722 1104 38-39 -0.7502288465520408 1104 40-41 -0.7867666953802583 1104 42-43 -0.6893155161948386 1104 44-45 -0.798319214448874 1104 46-47 -0.9577046227911694 1104 48-49 -0.9186328753331523 1104 50-51 -0.8570233632493682 1104 52-53 -0.822255366193744 1104 54-55 -0.789099582924301 1104 56-57 -0.7786349064121012 1104 58-59 -0.8460692603508022 1104 60-61 -0.8498990634025887 1104 62-63 -0.8789951189845269 1104 64-65 -0.936639523418684 1104 66-67 -0.9673357158506874 1104 68-69 -0.9643760766203542 1104 70-71 -0.9763025697611525 1104 72-73 -0.9398325121702271 1104 74-75 -0.9245308628042253 1104 76-77 -0.8412518277178087 1104 78-79 -0.6619405015693332 1104 80-81 -0.6521372093631257 1104 82-83 -0.7268971061390097 1104 84-85 -0.7972556488049314 1104 86-87 -0.8834861885316521 1104 88-89 -0.8130572542309906 1104 90-91 -0.9090636164456676 1104 92-93 -0.9103684287722764 1104 94-95 -0.9335188654248441 1104 96-97 -0.9497258745358366 1104 98-99 -1.1104418452036207 1104 100 -1.1073156363885701 1105 1 -0.43774251919829155 1105 2 -0.3954094172673521 1105 3 -0.47124065437227003 1105 4 -0.25212226072528665 1105 5 -0.32561692986353563 1105 6 -0.3793140971326068 1105 7 -0.42058630609400893 1105 8 -0.4585447710269648 1105 9 -0.558285865028104 1105 10-11 -0.5356772802367828 1105 12-13 -0.5622444271718621 1105 14-15 -0.7511375999117362 1105 16-17 -0.7054755102972123 1105 18-19 -0.7164499329034939 1105 20-21 -0.806365669563931 1105 22-23 -0.7309601458979103 1105 24-25 -0.7108023673966883 1105 26-27 -0.8201922041387917 1105 28-29 -0.8568296920298408 1105 30-31 -0.771305981715301 1105 32-33 -0.8255303257406794 1105 34-35 -0.8245965584291284 1105 36-37 -0.7574045385483146 1105 38-39 -0.8114263396116996 1105 40-41 -0.9220848152984473 1105 42-43 -0.9015718414311635 1105 44-45 -0.8685720177265992 1105 46-47 -0.7747422443533623 1105 48-49 -0.8000804621024997 1105 50-51 -0.905793172198095 1105 52-53 -0.8552094967891719 1105 54-55 -0.7915664895012213 1105 56-57 -0.8919801255517896 1105 58-59 -1.0260844561048117 1105 60-61 -0.9398676104057841 1105 62-63 -0.9517928478518982 1105 64-65 -0.9089310751907007 1105 66-67 -0.8879150084265675 1105 68-69 -0.8926342767619388 1105 70-71 -1.0202221939951102 1105 72-73 -0.9330956407471973 1105 74-75 -0.99468910008882 1105 76-77 -0.8737000509336639 1105 78-79 -0.8199268181100585 1105 80-81 -0.8707693178156077 1105 82-83 -0.8057785143770602 1105 84-85 -0.8235178661231437 1105 86-87 -0.8797125586369354 1105 88-89 -0.9117352712878493 1105 90-91 -0.9118758585040965 1105 92-93 -0.9821540615818414 1105 94-95 -0.9565509301469675 1105 96-97 -0.9433961415662075 1105 98-99 -1.1111515865615793 1105 100 -1.1176574660613348 1106 1 -0.5182326716121182 1106 2 -0.47142434094425667 1106 3 -0.5066052734394404 1106 4 -0.3229891051305742 1106 5 -0.33794003278978124 1106 6 -0.40597846945207294 1106 7 -0.5166782334377658 1106 8 -0.5021618507911327 1106 9 -0.6887778195625387 1106 10-11 -0.5866764750756133 1106 12-13 -0.6607958399397376 1106 14-15 -0.8085728366235685 1106 16-17 -0.7647902881965152 1106 18-19 -0.8138983772497497 1106 20-21 -0.7971586867204792 1106 22-23 -0.7541309718150728 1106 24-25 -0.8230707744379302 1106 26-27 -0.8693461189433194 1106 28-29 -0.8806662996114554 1106 30-31 -0.9387961944993606 1106 32-33 -0.9180909231293768 1106 34-35 -0.8876287998271479 1106 36-37 -0.9161458328855758 1106 38-39 -0.9179638025217969 1106 40-41 -0.9001786592127132 1106 42-43 -0.8756562157231684 1106 44-45 -0.9120348019461417 1106 46-47 -0.9578653318869641 1106 48-49 -0.9216722436237248 1106 50-51 -1.076740760593239 1106 52-53 -1.0182233174013646 1106 54-55 -1.0183492064231885 1106 56-57 -0.9900804625563637 1106 58-59 -1.032644833066719 1106 60-61 -1.2005680906749419 1106 62-63 -1.2277191049481715 1106 64-65 -1.0915997651536458 1106 66-67 -1.0634192740283979 1106 68-69 -1.0944903037256424 1106 70-71 -1.0538049843627206 1106 72-73 -1.1374469628602242 1106 74-75 -1.1326673733504506 1106 76-77 -1.103249295796985 1106 78-79 -0.9900142750392931 1106 80-81 -0.8746083562541216 1106 82-83 -0.8836823428868144 1106 84-85 -0.8886099089117465 1106 86-87 -0.9281139958144813 1106 88-89 -0.9789862882663947 1106 90-91 -0.9793300433186083 1106 92-93 -0.9682025990606711 1106 94-95 -0.9603387373294971 1106 96-97 -1.0154052624468406 1106 98-99 -0.9832102505139666 1106 100 -1.1117195187707445 1107 1 -0.4593578225407562 1107 2 -0.4569213297320651 1107 3 -0.4879574099939319 1107 4 -0.28656903267255984 1107 5 -0.39395109439811193 1107 6 -0.42140684915708704 1107 7 -0.4840918786437669 1107 8 -0.48002265571727065 1107 9 -0.5796770184025206 1107 10-11 -0.5794034093312987 1107 12-13 -0.594214958906008 1107 14-15 -0.7829740367534797 1107 16-17 -0.7702288253948026 1107 18-19 -0.724276164234972 1107 20-21 -0.7415627881150755 1107 22-23 -0.8086504276163353 1107 24-25 -0.843054783746517 1107 26-27 -0.8604099323521339 1107 28-29 -0.8253978053524875 1107 30-31 -0.8340856263207144 1107 32-33 -0.8042144900165695 1107 34-35 -0.8307883936132967 1107 36-37 -0.7951621906820492 1107 38-39 -0.8880804235482387 1107 40-41 -0.8829766897876254 1107 42-43 -0.8734101737566533 1107 44-45 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0.890454027362054 2311 32-33 1.0346836487983282 2311 34-35 1.0340315628270602 2311 36-37 1.0326111371313331 2311 38-39 1.0343962216386018 2311 40-41 1.0628005439944985 2311 42-43 1.0662403590390852 2311 44-45 1.0612446908833206 2311 46-47 0.7206394021586036 2311 48-49 1.011074268846663 2311 50-51 1.1791071858892934 2311 52-53 1.0447999550661748 2311 54-55 1.070925590371921 2311 56-57 1.1998032143777309 2311 58-59 1.1873817064265495 2311 60-61 1.2334260043981047 2311 62-63 1.253291562532766 2311 64-65 1.2906097854761427 2311 66-67 1.2922002633129708 2311 68-69 1.3061097814699139 2311 70-71 1.330147797748424 2311 72-73 1.3161679770626478 2311 74-75 1.3119355252573328 2311 76-77 1.2675464764048598 2311 78-79 0.9659695179259913 2311 80-81 0.9020130987146047 2311 82-83 0.9842295460343067 2311 84-85 1.0666283138154 2311 86-87 1.0886061903939641 2311 88-89 1.1697562287591445 2311 90-91 1.2241502816075247 2311 92-93 1.278036059631912 2311 94-95 1.2663153423218603 2311 96-97 1.2691781980754016 2311 98-99 1.3451923792807783 2311 100 1.4607476878233463 2312 1 0.23452415187783515 2312 2 0.38107124930832725 2312 3 0.46986088612649723 2312 4 0.25619059772114383 2312 5 0.41092244687115453 2312 6 0.44671172590218333 2312 7 0.33930603418276206 2312 8 0.4551778754205884 2312 9 0.6472845098438995 2312 10-11 0.6576672960497731 2312 12-13 0.7340504961816379 2312 14-15 0.9219170442437132 2312 16-17 0.9023421689856193 2312 18-19 0.8793300278967067 2312 20-21 0.9055846564338168 2312 22-23 0.9819386027790813 2312 24-25 0.9858913872797288 2312 26-27 1.0197683338611014 2312 28-29 1.0330226178946802 2312 30-31 1.0598406336545168 2312 32-33 0.9201739150138408 2312 34-35 1.0279657116758614 2312 36-37 1.0654208058062764 2312 38-39 0.9164270157633965 2312 40-41 1.0381225378987367 2312 42-43 1.1756081249631478 2312 44-45 0.9059489610979412 2312 46-47 0.9991231837883987 2312 48-49 1.1636212114836084 2312 50-51 1.0150452732715252 2312 52-53 1.1680913100857424 2312 54-55 1.0465476533656286 2312 56-57 1.0975426308057266 2312 58-59 1.2056164908771478 2312 60-61 1.2720367361178972 2312 62-63 1.3017781396263857 2312 64-65 1.2429445114301316 2312 66-67 1.2666108207224411 2312 68-69 1.3559976575733756 2312 70-71 1.2592860906031405 2312 72-73 1.2621175584926405 2312 74-75 1.310689861137007 2312 76-77 1.266269307669802 2312 78-79 1.1886716206002959 2312 80-81 1.1670889985822868 2312 82-83 1.1514602787936 2312 84-85 1.1899066227663866 2312 86-87 1.236673580134653 2312 88-89 1.2562386220931963 2312 90-91 1.277376928697695 2312 92-93 1.2972242249005568 2312 94-95 1.3371295166894015 2312 96-97 0.9496991506608019 2312 98-99 0.7465080831440964 2312 100 0.9733400690182279 2313 1 0.0737822595025932 2313 2 0.2583334572086855 2313 3 0.3495835769690743 2313 4 0.1983814887999813 2313 5 0.3399957245099401 2313 6 0.2471852702851578 2313 7 0.26217252724560325 2313 8 0.3843078550568322 2313 9 0.5595416423530963 2313 10-11 0.3933082926128506 2313 12-13 0.42021417003704187 2313 14-15 0.5284266518910385 2313 16-17 0.7793176910575212 2313 18-19 0.7099486235318082 2313 20-21 0.6538586860059183 2313 22-23 0.6935755001251778 2313 24-25 0.6016880476974862 2313 26-27 0.8010307518962847 2313 28-29 0.5722762131784478 2313 30-31 0.8444597503922182 2313 32-33 0.9398049140289331 2313 34-35 0.9676191718275859 2313 36-37 1.0200959615845946 2313 38-39 1.0237199975646476 2313 40-41 0.994880913154276 2313 42-43 1.0411536623571536 2313 44-45 0.9391340532231354 2313 46-47 0.984878642960112 2313 48-49 1.1018912289097145 2313 50-51 1.041044263883343 2313 52-53 1.048645508924892 2313 54-55 1.0059360882489088 2313 56-57 0.6663516625804178 2313 58-59 0.7552449637752545 2313 60-61 0.5778901554336002 2313 62-63 0.9778096561447924 2313 64-65 1.0834209180399554 2313 66-67 1.0269059053275171 2313 68-69 1.157939830004878 2313 70-71 1.1399818459458686 2313 72-73 1.1617936954735484 2313 74-75 1.175295250301275 2313 76-77 1.1247370198851314 2313 78-79 0.9655041041094066 2313 80-81 0.8953860278607664 2313 82-83 0.9373864857422589 2313 84-85 0.9783716831543501 2313 86-87 1.0307137304419278 2313 88-89 1.061964941598962 2313 90-91 0.916541428404134 2313 92-93 1.1568223380803921 2313 94-95 1.234975178219095 2313 96-97 1.3000826930284468 2313 98-99 1.3373465025650013 2313 100 1.4134268991622 2314 1 -0.33637052630444586 2314 2 0.06500318308732034 2314 3 0.31453299567204596 2314 4 0.2674134916961961 2314 5 0.34201052093926876 2314 6 0.3981755975503418 2314 7 0.46000979817245025 2314 8 0.4736757591398586 2314 9 0.4797196592860047 2314 10-11 0.5990065802238007 2314 12-13 0.4807736735081676 2314 14-15 0.7916165813363847 2314 16-17 0.8289495030640879 2314 18-19 0.8596846415196566 2314 20-21 0.8827044204408594 2314 22-23 0.9365151888365091 2314 24-25 0.8349903555287383 2314 26-27 0.7529547196532107 2314 28-29 0.8331315243926696 2314 30-31 0.8386339764342452 2314 32-33 0.9273199142738591 2314 34-35 0.9398851009314129 2314 36-37 0.8672423868490071 2314 38-39 0.6495045814956271 2314 40-41 0.6801312585474335 2314 42-43 0.8670545510103977 2314 44-45 0.9437207312433387 2314 46-47 0.9233972039177374 2314 48-49 0.9370319015896413 2314 50-51 0.873818803701397 2314 52-53 0.9430636195460878 2314 54-55 0.9110051100556333 2314 56-57 0.8679183686147454 2314 58-59 1.0227729967810788 2314 60-61 1.0964832480883118 2314 62-63 1.0339993795806706 2314 64-65 1.0141476596310568 2314 66-67 0.7744402833389401 2314 68-69 0.7400097728120727 2314 70-71 0.8970006442387799 2314 72-73 1.0146054173409027 2314 74-75 1.049701453517116 2314 76-77 0.8881840925328959 2314 78-79 0.8398677603259088 2314 80-81 0.8835473163061849 2314 82-83 0.8487670384044108 2314 84-85 0.9082513510331154 2314 86-87 0.9387770018220785 2314 88-89 0.960470315504363 2314 90-91 1.0482654008077823 2314 92-93 0.8851113200332001 2314 94-95 0.9391957488408913 2314 96-97 1.0099569254651115 2314 98-99 1.1742912237627472 2314 100 1.213890535576187 2315 1 0.4358640695628111 2315 2 0.45952070854827554 2315 3 0.44825054519432683 2315 4 0.32172147251256433 2315 5 0.4002931592642227 2315 6 0.43123842559045045 2315 7 0.39121499647528424 2315 8 0.3153707216152597 2315 9 0.5385451286270708 2315 10-11 0.5674706969170344 2315 12-13 0.4592487549921813 2315 14-15 0.6716173939339711 2315 16-17 0.796839392925456 2315 18-19 0.8099902098709819 2315 20-21 0.560921647298585 2315 22-23 0.8959881902817699 2315 24-25 0.9565711137456034 2315 26-27 0.8825101733702425 2315 28-29 0.8713411555736243 2315 30-31 0.9181857761004437 2315 32-33 0.8624612112745851 2315 34-35 0.9518441233237454 2315 36-37 0.9415066166727115 2315 38-39 0.952741635206749 2315 40-41 0.9790675867777594 2315 42-43 1.0697185915094138 2315 44-45 1.0954424355665182 2315 46-47 1.0898439415758787 2315 48-49 0.9744416913854295 2315 50-51 1.0919521034755064 2315 52-53 1.0890754803048566 2315 54-55 0.9167830086677284 2315 56-57 0.9476384788747367 2315 58-59 0.9342561878834488 2315 60-61 1.044438404310192 2315 62-63 1.1067830724356682 2315 64-65 1.166565533189818 2315 66-67 1.191076926107847 2315 68-69 1.2394097961346802 2315 70-71 1.1338808077291986 2315 72-73 1.2003282228921037 2315 74-75 0.6065355311159593 2315 76-77 0.6481377401726434 2315 78-79 0.8897966052712079 2315 80-81 0.926741336643282 2315 82-83 0.9181588354956247 2315 84-85 0.941912757449586 2315 86-87 0.9501154819585587 2315 88-89 0.9332454998294573 2315 90-91 1.0378095256480968 2315 92-93 1.0788357713583387 2315 94-95 1.058985890238148 2315 96-97 1.190050672315639 2315 98-99 1.2484666878535862 2315 100 1.172615274476449 2316 1 0.3110744514478654 2316 2 0.10569528933498162 2316 3 0.2570456716835423 2316 4 0.046117235198998685 2316 5 0.3067565629193538 2316 6 0.39694779549512305 2316 7 0.4544770076987632 2316 8 0.45491558052152214 2316 9 0.6488710630000583 2316 10-11 0.6270046857869218 2316 12-13 0.4103579321704416 2316 14-15 0.5916988858631385 2316 16-17 0.8112879386412928 2316 18-19 0.7541402297104653 2316 20-21 0.7273368360070833 2316 22-23 0.8047487060416572 2316 24-25 0.5344326508220902 2316 26-27 0.76132633971514 2316 28-29 0.9353812079898702 2316 30-31 0.5361494781814287 2316 32-33 0.6827803101952483 2316 34-35 0.8483020919606972 2316 36-37 0.9015771051908175 2316 38-39 0.8367317388055326 2316 40-41 0.8561811586517081 2316 42-43 0.7959581257650399 2316 44-45 0.7119394829845547 2316 46-47 0.829766650949125 2316 48-49 0.941624518061694 2316 50-51 0.958569621572785 2316 52-53 0.9518883522951711 2316 54-55 0.8220911875365076 2316 56-57 0.9899097162916703 2316 58-59 0.897787682646829 2316 60-61 0.9821999103770693 2316 62-63 0.9943732920704136 2316 64-65 1.0554794965184513 2316 66-67 1.0501242307722976 2316 68-69 0.9685833732769282 2316 70-71 0.9992582080566308 2316 72-73 0.9647774688250124 2316 74-75 0.9283516274391559 2316 76-77 0.853164431780371 2316 78-79 0.6552179525555388 2316 80-81 0.6354640406815406 2316 82-83 0.5739121857008556 2316 84-85 0.6804353661249394 2316 86-87 0.722929320915302 2316 88-89 0.6672395046376174 2316 90-91 0.5813512235744547 2316 92-93 0.4594605650443846 2316 94-95 0.46304921653596764 2316 96-97 0.7239650524401426 2316 98-99 0.8764382256969832 2316 100 0.517314531246047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 40.0 13 201.0 14 759.0 15 2179.0 16 5054.0 17 10285.0 18 17271.0 19 25456.0 20 36774.0 21 51260.0 22 63163.0 23 66004.0 24 67644.0 25 73101.0 26 86028.0 27 105854.0 28 131684.0 29 161947.0 30 201130.0 31 251547.0 32 319613.0 33 421457.0 34 603410.0 35 845334.0 36 1322497.0 37 1962849.0 38 1598848.0 39 234801.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.265258936805004 20.255965296848355 13.76616378066345 23.712611985683196 2 15.478164342795559 19.871962558048697 40.88958097623934 23.760292122916407 3 19.030649481924925 25.42160562598551 29.31432003475566 26.233424857333908 4 12.675973262198747 16.37314030650819 38.20888820657964 32.74199822471342 5 13.329106189957207 37.694772169857316 34.508836902846284 14.467284737339195 6 31.16122782552706 37.01891771667331 17.596747830511937 14.22310662728769 7 27.79548140545982 31.87889980159577 22.13312584394493 18.192492948999476 8 26.253237428335208 35.8963399823337 19.472272497278222 18.378150092052874 9 26.20603556426568 15.831622577066545 20.8963920459037 37.065949812764075 10-11 25.819211820016903 26.136196909353547 27.227150415341566 20.817440855287977 12-13 25.76338987244026 23.620643339755976 27.908132663236795 22.70783412456697 14-15 22.98953497979046 25.086000744962845 25.645478689400804 26.278985585845895 16-17 22.175010010165945 27.842742889320448 26.672418906116757 23.309828194396847 18-19 21.811286703595513 27.35245432983142 28.85930593690556 21.976953029667506 20-21 22.916093800140846 26.594113253389917 28.59521223399425 21.894580712474987 22-23 22.952350016702795 26.38049598354068 28.3426674736318 22.324486526124726 24-25 22.51691465441302 26.29412078299093 28.92464754261233 22.26431701998372 26-27 22.484884483383823 26.596374237661298 28.286733106955168 22.632008171999708 28-29 22.045408244892528 26.95431210548243 28.60520840647428 22.395071243150763 30-31 22.753264290037002 26.598141486426595 28.563226577763025 22.085367645773378 32-33 22.866182941165913 26.759389793339235 28.04248588890317 22.331941376591686 34-35 22.824757304777485 26.723214421229812 28.47197851755502 21.980049756437683 36-37 22.641100435333414 26.50623624307223 28.283473033896634 22.569190287697726 38-39 22.645736597927616 26.669148494519995 28.538154150248264 22.146960757304125 40-41 22.549856483877203 26.07115231021753 28.865546720297868 22.513444485607394 42-43 22.46061368478578 26.293795394710116 28.374947843393556 22.870643077110543 44-45 22.4508963511925 26.83904269494147 28.336323440245526 22.373737513620508 46-47 22.495126881132947 26.613902701276587 27.869542659402878 23.02142775818759 48-49 22.930465539199123 26.575499797165698 27.899851147143234 22.59418351649195 50-51 22.716448151304004 26.61160116841319 27.806632152204614 22.865318528078195 52-53 22.42509231198795 26.957483544598 27.552643640038266 23.064780503375786 54-55 22.376652432549893 26.883543167595096 27.60889341676619 23.130910983088818 56-57 22.935955973214956 26.638170063184706 27.29645325811987 23.129420705480467 58-59 22.312049436247698 26.915657771797203 27.774752037210703 22.997540754744396 60-61 22.555107825220887 27.256499438338487 27.38280620505431 22.805586531386318 62-63 22.692806142000883 26.845111775180097 27.220788218369485 23.24129386444954 64-65 22.221625188123458 26.860987991265116 27.4052272175828 23.51215960302863 66-67 22.450614284145686 26.663795192665283 27.387759946620832 23.497830576568195 68-69 22.19637035648538 26.74012424636436 27.543653960291525 23.51985143685873 70-71 22.211483489790567 26.43196696480359 27.61835541652351 23.738194128882338 72-73 22.492108626489458 26.640304944167877 27.607870513016913 23.259715916325753 74-75 22.635272326914656 26.90406981106419 27.260412316345757 23.200245545675397 76-77 22.501692442386258 26.75974474563731 27.112205611694563 23.626357200281873 78-79 22.3394433367848 26.751488416044655 27.228896390917118 23.680171856253423 80-81 22.12759181236108 27.080914308462994 27.3772928749771 23.414201004198826 82-83 22.702112739057537 26.50804433837301 27.944158583850143 22.845684338719312 84-85 22.827495499645426 26.76957232128246 27.17235730260999 23.23057487646213 86-87 22.031835764350852 27.208598142127688 27.44869901774658 23.310867075774883 88-89 22.476671382667842 26.49446767162429 27.43170578849933 23.597155157208537 90-91 22.46960222049758 26.43315692149264 27.503207247499066 23.59403361051071 92-93 22.571097501483926 26.63518885647194 27.365920712673073 23.42779292937105 94-95 21.929082321024055 26.584372070500372 27.721030909203588 23.765514699271986 96-97 22.05928723012097 26.56223738651852 27.492441203284624 23.88603418007589 98-99 21.698153276461 26.86672511327493 27.373654798912046 24.06146681135203 100 22.050980400757343 26.549270746389976 27.055443404917234 24.344305447935447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1581.0 1 1106.5 2 1266.0 3 1814.0 4 1325.5 5 1019.0 6 1481.0 7 2839.0 8 4574.0 9 5314.0 10 4669.0 11 4059.5 12 4374.0 13 5019.0 14 5763.5 15 6721.0 16 7932.5 17 8966.5 18 9977.5 19 10996.0 20 12034.5 21 13304.5 22 15003.5 23 17570.0 24 20892.5 25 24774.5 26 30287.5 27 37263.5 28 46499.5 29 60917.5 30 79576.0 31 97949.5 32 120893.5 33 145264.0 34 165421.5 35 187047.0 36 207736.5 37 235569.0 38 252447.0 39 251301.5 40 247469.5 41 242786.0 42 246395.0 43 258982.5 44 278504.0 45 311430.0 46 340819.0 47 360896.5 48 379106.0 49 383187.0 50 374918.5 51 374387.0 52 371098.0 53 353134.0 54 334333.0 55 300902.5 56 265775.5 57 227410.5 58 189506.5 59 170593.5 60 141548.5 61 106158.0 62 81162.0 63 64022.5 64 46348.5 65 28534.5 66 19324.0 67 14122.5 68 10686.0 69 8019.0 70 6132.0 71 4690.5 72 3472.0 73 2663.5 74 2088.5 75 1493.5 76 943.0 77 547.5 78 323.0 79 195.5 80 120.0 81 77.5 82 50.0 83 33.5 84 21.5 85 11.0 86 8.5 87 6.5 88 7.0 89 5.5 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04554468127088931 2 0.0 3 0.0 4 0.032597852695784725 5 0.0 6 0.0 7 7.961953401802287E-4 8 0.0 9 0.010892875378697622 10-11 3.9809767009011434E-4 12-13 1.1539062901162734E-5 14-15 1.2692969191279007E-4 16-17 2.8847657252906834E-4 18-19 0.0 20-21 2.3078125802325468E-5 22-23 0.0 24-25 7.038828369709268E-4 26-27 4.3271485879360253E-4 28-29 1.557773491656969E-4 30-31 0.0023481993003866162 32-33 0.015168098183578413 34-35 0.02550709854302022 36-37 0.030295809647002757 38-39 0.02194729763801152 40-41 0.0013327617650842956 42-43 8.077344030813913E-5 44-45 0.01122750820283134 46-47 0.018341340481398166 48-49 0.00316747276636917 50-51 0.01106019179076448 52-53 0.010379387079595879 54-55 0.010200531604627857 56-57 4.1540626444185844E-4 58-59 0.008671605770223793 60-61 0.004713707195124977 62-63 0.0013154531707325516 64-65 0.03485950902441262 66-67 0.05951848644419738 68-69 0.059039615333799124 70-71 0.05103727521184277 72-73 0.045094657817743966 74-75 0.035321071540459124 76-77 0.031155469833139382 78-79 0.014989242708610392 80-81 0.0011827539473691802 82-83 0.037963516944825394 84-85 0.05074302910786312 86-87 0.05602791991659565 88-89 0.06903244380620606 90-91 0.04837752121312476 92-93 0.03915204042364516 94-95 0.012992984826709237 96-97 0.0036751915340203305 98-99 0.021889602323505706 100 0.031940126110418444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8666215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.29548296593489 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31637572355177 46.91870000942471 2 14.72088012906913 18.340886746479907 3 4.845252260244954 9.055119889312412 4 2.0287706434564003 5.055329882449077 5 1.0160700285279933 3.1648286577181293 6 0.5726047212723127 2.1402412596139944 7 0.3482093570613941 1.5184309049978049 8 0.24471407168846002 1.2195665027514424 9 0.16138098045257254 0.9047975506928194 >10 0.7008113811919972 7.3428502122124515 >50 0.030510346215503147 1.300638168413738 >100 0.01327009627774269 1.4734446826936045 >500 7.189394578380715E-4 0.2899595505851282 >1k 3.3547230397418563E-4 0.338255678112514 >5k 2.396230699273953E-5 0.07581644170933706 >10k+ 7.18869209782186E-5 0.8611338628328875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24501 0.2827185801413881 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20321 0.23448529721452788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12866 0.14846158328635972 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.1539062901162734E-5 5 0.0 0.0 0.0 0.0 1.1539062901162734E-5 6 0.0 0.0 0.0 0.0 2.3078125802325468E-5 7 0.0 0.0 0.0 0.0 3.46171887034882E-5 8 0.0 0.0 0.0 0.0 3.46171887034882E-5 9 0.0 0.0 0.0 1.1539062901162734E-5 3.46171887034882E-5 10-11 0.0 0.0 0.0 2.3078125802325468E-5 3.46171887034882E-5 12-13 0.0 0.0 0.0 3.46171887034882E-5 3.46171887034882E-5 14-15 0.0 0.0 0.0 3.46171887034882E-5 8.077344030813915E-5 16-17 0.0 0.0 0.0 5.19257830552323E-5 1.384687548139528E-4 18-19 0.0 0.0 0.0 9.231250320930187E-5 1.384687548139528E-4 20-21 0.0 0.0 0.0 1.7885547496802239E-4 1.5000781771511552E-4 22-23 0.0 0.0 0.0 3.1732422978197514E-4 2.0193360077034784E-4 24-25 0.0 0.0 0.0 6.808047111686014E-4 2.5385938382558015E-4 26-27 0.0 0.0 0.0 0.0016443164634156896 2.6539844672674286E-4 28-29 0.0 0.0 0.0 0.004038672015406957 2.6539844672674286E-4 30-31 0.0 0.0 0.0 0.01464884035302609 2.6539844672674286E-4 32-33 0.0 0.0 0.0 0.0403463334339155 2.8270704107848695E-4 34-35 5.769531450581367E-6 0.0 0.0 0.07801560427476124 2.8847657252906834E-4 36-37 1.1539062901162734E-5 0.0 0.0 0.14002652830560977 2.8847657252906834E-4 38-39 1.1539062901162734E-5 0.0 0.0 0.23146206273442327 2.942461039796497E-4 40-41 1.1539062901162734E-5 0.0 0.0 0.3293594723878879 3.0001563543023105E-4 42-43 1.1539062901162734E-5 1.1539062901162734E-5 0.0 0.4250356124328787 3.1732422978197514E-4 44-45 1.1539062901162734E-5 1.1539062901162734E-5 0.0 0.5401896906550322 3.346328241337193E-4 46-47 1.1539062901162734E-5 1.1539062901162734E-5 0.0 0.677279527452296 3.346328241337193E-4 48-49 1.1539062901162734E-5 1.1539062901162734E-5 0.0 0.82469105601465 3.46171887034882E-4 50-51 1.1539062901162734E-5 1.1539062901162734E-5 0.0 0.9753969870352859 3.46171887034882E-4 52-53 1.1539062901162734E-5 1.1539062901162734E-5 0.0 1.1207314842754306 3.5771094993604477E-4 54-55 1.1539062901162734E-5 1.1539062901162734E-5 0.0 1.283859216509168 3.692500128372075E-4 56-57 1.1539062901162734E-5 1.1539062901162734E-5 0.0 1.4723555785311118 3.807890757383702E-4 58-59 1.1539062901162734E-5 1.1539062901162734E-5 0.0 1.6870975391217504 4.038672015406957E-4 60-61 1.73085943517441E-5 1.1539062901162734E-5 0.0 1.9039511482233018 4.038672015406957E-4 62-63 2.3078125802325468E-5 1.1539062901162734E-5 0.0 2.1199739447959693 4.1540626444185844E-4 64-65 2.3078125802325468E-5 1.1539062901162734E-5 0.0 2.3388238117794216 4.1540626444185844E-4 66-67 2.3078125802325468E-5 1.1539062901162734E-5 0.0 2.5860136172481294 4.211757958924398E-4 68-69 2.3078125802325468E-5 1.1539062901162734E-5 0.0 2.8616010565165992 4.2694532734302115E-4 70-71 2.3078125802325468E-5 1.73085943517441E-5 0.0 3.156395265984054 4.6156251604650934E-4 72-73 2.3078125802325468E-5 2.3078125802325468E-5 0.0 3.449418229296181 5.884922079592994E-4 74-75 2.3078125802325468E-5 2.3078125802325468E-5 0.0 3.736983215856057 5.884922079592994E-4 76-77 2.3078125802325468E-5 2.3078125802325468E-5 0.0 4.060503922415957 5.884922079592994E-4 78-79 2.3078125802325468E-5 2.3078125802325468E-5 0.0 4.41244534090142 5.884922079592994E-4 80-81 2.3078125802325468E-5 2.3078125802325468E-5 0.0 4.781943443591002 6.28878928113369E-4 82-83 2.3078125802325468E-5 2.3078125802325468E-5 0.0 5.155878315966081 6.692656482674386E-4 84-85 2.3078125802325468E-5 2.8847657252906834E-5 0.0 5.52493216473397 6.692656482674386E-4 86-87 2.3078125802325468E-5 3.46171887034882E-5 0.0 5.915142885331139 6.692656482674386E-4 88 2.3078125802325468E-5 3.46171887034882E-5 0.0 6.227851489952649 6.692656482674386E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14615 0.0 46.426373 1 GTATCAA 24520 0.0 38.487896 1 GTACTAG 5735 0.0 31.976316 1 CAACGCA 29050 0.0 31.249086 5 ATCAACG 29190 0.0 31.047543 3 TACCTGG 6415 0.0 30.919146 2 TATCAAC 31315 0.0 29.808392 2 AACGCAG 32860 0.0 28.140635 6 TCAACGC 32715 0.0 28.061419 4 GTGGTAT 5535 0.0 26.930157 1 TAGTACT 8290 0.0 26.76343 4 CTGTGCG 8065 0.0 26.333307 9 TAGGCAT 7665 0.0 25.991423 5 GGGCACC 7225 0.0 25.897013 94 GTACCTG 7820 0.0 25.615337 1 ACGCAGA 35685 0.0 25.504704 7 GTACATG 31500 0.0 25.108032 1 CGCAGAG 36300 0.0 25.00759 8 ACCTGGG 7830 0.0 24.673704 3 CTAGGCA 8325 0.0 24.618214 4 >>END_MODULE