FastQCFastQC Report
Mon 6 Jun 2016
ERR1211210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845144
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400970.45332218446641454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304300.34403057768194617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192960.2181535993082758No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC117350.13267166707517708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196200.046.5130121
TACCTGG38150.040.4638822
GTATCAA347950.040.28131
GTACTAG101250.038.9068531
GCATCGT99800.037.066538
ACCTGGG42100.036.1765943
GGGCACC103150.035.5377194
TAGGCAT126200.033.9699825
CAACGCA403250.033.199035
ATCAACG410150.032.6867373
TATCAAC447900.031.1342832
GTGGTAT58600.030.6804281
CTAGGCA142100.030.5655924
TATTCTC42650.030.3091345
CTGTGCG136650.030.0292729
AACGCAG461150.029.887126
ACTGTGC141150.029.4713558
TAGTACT142850.028.990164
TACCCCG5850.028.9272045
GTACATG486800.028.6468411
Produced by FastQC (version 0.11.5)