FastQCFastQC Report
Mon 6 Jun 2016
ERR1211210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845144
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363340.410779067022538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303870.34354443522909295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192060.2171360918488156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208700.046.1838841
TACCTGG42450.045.8334732
ACCTGGG42750.043.7605973
GTATCAA365650.039.450171
CCTGGGG42600.037.515054
GTACTAG97600.037.4790731
GGGCACC99250.036.9471994
CTGGGGG42250.034.366515
TAGGCAT121050.034.043835
CAACGCA412100.033.363795
ATCAACG419100.032.7716873
GTGGTAT58450.031.8544241
TACCCCG4500.031.3265365
CTAGGCA134350.030.9279294
TATCAAC467750.030.7244262
AACGCAG473500.030.0000386
CTGTGCG136250.029.969849
GTACCTG65850.029.7027471
TAGTACT140550.029.061984
AAGCTAA59550.029.0380349
Produced by FastQC (version 0.11.5)