FastQCFastQC Report
Mon 6 Jun 2016
ERR1211209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251321
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398460.3541450821641299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312250.27752296819191274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193780.17222866541626536No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC166510.14799151139675065No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA141390.12566524410778077No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC131250.039.1382494
GTACTAG139900.038.6816861
GGTATCA294500.035.8241231
GCATCGT143350.035.3155568
GTATCAA468800.034.570111
ACCTGGG42900.034.5161673
TACCTGG44950.034.233022
TAGGCAT171800.033.9288945
TATTCTC54350.031.1368125
CTAGGCA192200.031.0122434
CTGTGCG186900.030.959299
CAACGCA503450.030.2804135
ACTGTGC200400.029.8592388
TAGTACT194900.029.7867034
ATCAACG514200.029.7112263
TATCAAC566850.028.44942
TACTGTG223000.028.2046387
GTACTGT209900.028.016646
CATCGTC184600.027.7292699
AAGCTAA73550.027.672449
Produced by FastQC (version 0.11.5)